TDMS Study 05134-02 Pathology Tables
NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 Facility: Battelle Northwest Chemical CAS #: 106-99-0 Lock Date: None Cage Range: All Reasons For Removal: All Removal Date Range: All Treatment Groups: Include All Note: Animals arranged according to CID number Page 1 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 7| 7| 7| 7| 7| 7| 7| 2| 7| 7| 4| 4| 7| 2| 7| 7| 7| 7| 6| 2| 7| 7| 4| 7| DAY ON TEST | 5| 3| 3| 3| 3| 3| 3| 0| 8| 3| 3| 5| 5| 3| 8| 3| 3| 3| 3| 2| 8| 3| 3| 5| 3| | 7| 7| 7| 7| 7| 7| 7| 8| 2| 7| 7| 7| 7| 7| 2| 7| 7| 7| 7| 5| 2| 7| 7| 7| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| CONTROL | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + M + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Mixed | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X | Hepatocellular Carcinoma | | Hepatocellular Adenoma | X X X X X X| Hepatocellular Adenoma, Multiple | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | | __________________________________________________________________________| Mesentery | + + + + | Fat, Lymphoma Malignant Lymphocytic | | Fat, Lymphoma Malignant Mixed | | Fat, Sarcoma, Metastatic, Skin | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Sarcoma, Metastatic, Skin | | Acinus, Histiocytic Sarcoma | | Acinus, Lymphoma Malignant | | Lymphocytic | | Acinus, Lymphoma Malignant Mixed | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Leiomyoma | | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Tooth | + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Blood Vessel | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| Page 2 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 7| 7| 7| 7| 7| 7| 7| 2| 7| 7| 4| 4| 7| 2| 7| 7| 7| 7| 6| 2| 7| 7| 4| 7| DAY ON TEST | 5| 3| 3| 3| 3| 3| 3| 0| 8| 3| 3| 5| 5| 3| 8| 3| 3| 3| 3| 2| 8| 3| 3| 5| 3| | 7| 7| 7| 7| 7| 7| 7| 8| 2| 7| 7| 7| 7| 7| 2| 7| 7| 7| 7| 5| 2| 7| 7| 7| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| CONTROL | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Capsule, Adenoma | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + M + + + + + + + + M M + M + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Pars Distalis, Adenoma | X X X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + M + + + +| Follicular Cell, Adenoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | M | __________________________________________________________________________| Ovary | + + + + + I + + + + + + + + + + + + + + + + + + +| Adenoma | X | Granulosa Cell Tumor Benign | X| Hemangioma | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | | Thecoma Benign | X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangioma | X | Polyp Stromal | X X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Spleen | X | Histiocytic Sarcoma | | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | | Iliac, Lymphoma Malignant Lymphocytic| | Iliac, Lymphoma Malignant Mixed | X | Pancreatic, Lymphoma Malignant Mixed | | Renal, Lymphoma Malignant Mixed | X | _____________________________________________________________________________________________________________________| Page 3 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 7| 7| 7| 7| 7| 7| 7| 2| 7| 7| 4| 4| 7| 2| 7| 7| 7| 7| 6| 2| 7| 7| 4| 7| DAY ON TEST | 5| 3| 3| 3| 3| 3| 3| 0| 8| 3| 3| 5| 5| 3| 8| 3| 3| 3| 3| 2| 8| 3| 3| 5| 3| | 7| 7| 7| 7| 7| 7| 7| 8| 2| 7| 7| 7| 7| 7| 2| 7| 7| 7| 7| 5| 2| 7| 7| 7| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| CONTROL | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | __________________________________________________________________________| Lymph Node, Bronchial | M + + + + + + + + + M M + + + + + M + + + + + M +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node, Mandibular | + + + M + + M + M + + + + + + + + M M M + + + M +| Histiocytic Sarcoma | | Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node, Mediastinal | + M + + M M I + M M M M + + M M + + + + + M + M +| Histiocytic Sarcoma | | Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X X | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + + + + M + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Mixed | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Hemangiosarcoma | X | Subcutaneous Tissue, Sarcoma | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | + | Hemangiosarcoma, Metastatic, Spleen | X | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| Page 4 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 7| 7| 7| 7| 7| 7| 7| 2| 7| 7| 4| 4| 7| 2| 7| 7| 7| 7| 6| 2| 7| 7| 4| 7| DAY ON TEST | 5| 3| 3| 3| 3| 3| 3| 0| 8| 3| 3| 5| 5| 3| 8| 3| 3| 3| 3| 2| 8| 3| 3| 5| 3| | 7| 7| 7| 7| 7| 7| 7| 8| 2| 7| 7| 7| 7| 7| 2| 7| 7| 7| 7| 5| 2| 7| 7| 7| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| CONTROL | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Mediastinum, Sarcoma, Metastatic, | | Skin | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + M + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + + | __________________________________________________________________________| Harderian Gland | + + + + + + + + I + + + + + + + + + + + M + + + +| Adenoma | X X X X X X| _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X X | _____________________________________________________________________________________________________________________| Page 5 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 7| 7| 7| 5| 7| 7| 5| 7| 4| 7| 7| 2| 7| 7| 4| 4| 7| 7| 4| 4| 7| 7| 2| 7| DAY ON TEST | 7| 3| 3| 1| 5| 3| 3| 6| 3| 5| 3| 3| 8| 3| 3| 5| 5| 3| 3| 5| 5| 3| 3| 8| 0| | 7| 7| 7| 4| 1| 7| 7| 0| 7| 7| 7| 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 2| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| CONTROL | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + M + + M M + M + + + + + + + + M + + + M + + +| __________________________________________________________________________| Gallbladder | + + + A + + + A + + + I + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large | + + + + + + + A + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + A + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + A + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + A + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + A + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + A + + + A + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + A + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + A + + + A + + + + + + + + + + + + + + + + +| Lymphoma Malignant Mixed | X | __________________________________________________________________________| Liver | + + + + + + + A + + + + + + + + + + + + + + + + +| Hemangiosarcoma | | Hepatocellular Carcinoma | X X | Hepatocellular Adenoma | X X X X | Hepatocellular Adenoma, Multiple | X | Histiocytic Sarcoma | X X| Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Mesentery | + + + + + + | Fat, Lymphoma Malignant Lymphocytic | | Fat, Lymphoma Malignant Mixed | | Fat, Sarcoma, Metastatic, Skin | X | __________________________________________________________________________| Pancreas | + + + + + + + A + + + + + + + + + + + + + + + + +| Sarcoma, Metastatic, Skin | X | Acinus, Histiocytic Sarcoma | X| Acinus, Lymphoma Malignant | | Lymphocytic | | Acinus, Lymphoma Malignant Mixed | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Leiomyoma | X | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + A + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Tooth | + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Blood Vessel | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| Page 6 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 7| 7| 7| 5| 7| 7| 5| 7| 4| 7| 7| 2| 7| 7| 4| 4| 7| 7| 4| 4| 7| 7| 2| 7| DAY ON TEST | 7| 3| 3| 1| 5| 3| 3| 6| 3| 5| 3| 3| 8| 3| 3| 5| 5| 3| 3| 5| 5| 3| 3| 8| 0| | 7| 7| 7| 4| 1| 7| 7| 0| 7| 7| 7| 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 2| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| CONTROL | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X| Capsule, Adenoma | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + A + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | M + + M M + + + + + + + + I M + + + + + + + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X| Pars Distalis, Adenoma | X X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicular Cell, Adenoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | Granulosa Cell Tumor Benign | | Hemangioma | X | Histiocytic Sarcoma | X| Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | | Thecoma Benign | | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangioma | | Polyp Stromal | X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Spleen | | Histiocytic Sarcoma | | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | | Iliac, Lymphoma Malignant Lymphocytic| | Iliac, Lymphoma Malignant Mixed | | Pancreatic, Lymphoma Malignant Mixed | | Renal, Lymphoma Malignant Mixed | | _____________________________________________________________________________________________________________________| Page 7 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 7| 7| 7| 5| 7| 7| 5| 7| 4| 7| 7| 2| 7| 7| 4| 4| 7| 7| 4| 4| 7| 7| 2| 7| DAY ON TEST | 7| 3| 3| 1| 5| 3| 3| 6| 3| 5| 3| 3| 8| 3| 3| 5| 5| 3| 3| 5| 5| 3| 3| 8| 0| | 7| 7| 7| 4| 1| 7| 7| 0| 7| 7| 7| 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 2| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| CONTROL | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | __________________________________________________________________________| Lymph Node, Bronchial | M M + + + + + + + M + + + + M + + + + + + + + + +| Histiocytic Sarcoma | X| Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node, Mandibular | + M + + + + M + + + + + + + + M + + + + + + + I +| Histiocytic Sarcoma | X| Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node, Mediastinal | + + M M + + + + M M + M M + + M M + M + + M + M +| Histiocytic Sarcoma | X| Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + A + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X| Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Spleen | + + + + + + + A + + + + + + + + + + + + + + + + +| Hemangiosarcoma | | Histiocytic Sarcoma | X| Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | | __________________________________________________________________________| Thymus | M + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X| Lymphoma Malignant Mixed | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Hemangiosarcoma | | Subcutaneous Tissue, Sarcoma | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | | Hemangiosarcoma, Metastatic, Spleen | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X X | Histiocytic Sarcoma | X| Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| Page 8 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 7| 7| 7| 5| 7| 7| 5| 7| 4| 7| 7| 2| 7| 7| 4| 4| 7| 7| 4| 4| 7| 7| 2| 7| DAY ON TEST | 7| 3| 3| 1| 5| 3| 3| 6| 3| 5| 3| 3| 8| 3| 3| 5| 5| 3| 3| 5| 5| 3| 3| 8| 0| | 7| 7| 7| 4| 1| 7| 7| 0| 7| 7| 7| 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 2| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| CONTROL | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Mediastinum, Sarcoma, Metastatic, | | Skin | X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + M + + M +| Adenoma | X X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + A + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X| Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Urinary Bladder | + + I + + + + A + + + + + + + + + + + + + + M + +| Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X| Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | X | _____________________________________________________________________________________________________________________| Page 9 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 4| 7| 7| 2| 2| 7| 2| 6| 7| 4| 2| 2| 7| 7| 4| 6| | | DAY ON TEST | 3| 2| 3| 3| 5| 3| 3| 8| 8| 3| 8| 4| 3| 0| 8| 8| 3| 0| 8| 5| | | | 7| 3| 7| 7| 7| 7| 7| 2| 2| 7| 2| 4| 7| 1| 2| 2| 7| 8| 5| 8| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | CONTROL | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + M + + + + + + + + + + + + + + M + | 61 | __________________________________________________________________________|____________| Gallbladder | + A + + + + + + + + + + + + + + + + + + | 66 | __________________________________________________________________________|____________| Intestine Large | + A + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Intestine Large, Cecum | + A + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Intestine Large, Colon | + A + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Intestine Large, Rectum | + A + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Intestine Small | + A + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + A + + + + + + + + + + + + + + + M + + | 66 | __________________________________________________________________________|____________| Intestine Small, Ileum | + A + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + A + + + + + + + + + + + + + + + + + + | 67 | Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + | 69 | Hemangiosarcoma | | 1 | Hepatocellular Carcinoma | X X | 4 | Hepatocellular Adenoma | X | 11 | Hepatocellular Adenoma, Multiple | | 1 | Histiocytic Sarcoma | X | 3 | Lymphoma Malignant Lymphocytic | X | 1 | Lymphoma Malignant Mixed | X | 2 | __________________________________________________________________________|____________| Mesentery | + + | 12 | Fat, Lymphoma Malignant Lymphocytic | X | 1 | Fat, Lymphoma Malignant Mixed | X | 1 | Fat, Sarcoma, Metastatic, Skin | | 1 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + | 69 | Sarcoma, Metastatic, Skin | | 1 | Acinus, Histiocytic Sarcoma | X | 2 | Acinus, Lymphoma Malignant | | | Lymphocytic | X | 1 | Acinus, Lymphoma Malignant Mixed | X X | 2 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + | 70 | Leiomyoma | | 1 | Lymphoma Malignant Lymphocytic | X | 1 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + | 69 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X | 1 | __________________________________________________________________________|____________| Tooth | + + | 4 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 10 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 4| 7| 7| 2| 2| 7| 2| 6| 7| 4| 2| 2| 7| 7| 4| 6| | | DAY ON TEST | 3| 2| 3| 3| 5| 3| 3| 8| 8| 3| 8| 4| 3| 0| 8| 8| 3| 0| 8| 5| | | | 7| 3| 7| 7| 7| 7| 7| 2| 2| 7| 2| 4| 7| 1| 2| 2| 7| 8| 5| 8| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | CONTROL | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ CARDIOVASCULAR SYSTEM - cont | | | | | | | | | __________________________________________________________________________|____________| Blood Vessel | + | 1 | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + | 70 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant Lymphocytic | X | 1 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + | 70 | Histiocytic Sarcoma | X | 2 | Capsule, Adenoma | X | 1 | __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + | 70 | Histiocytic Sarcoma | | 1 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + M M + + M + M + + + + + + | 57 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + | 70 | Histiocytic Sarcoma | | 1 | Pars Distalis, Adenoma | X X | 7 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + | 69 | Follicular Cell, Adenoma | | 1 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Clitoral Gland | | | __________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + | 69 | Adenoma | X | 2 | Granulosa Cell Tumor Benign | | 1 | Hemangioma | | 1 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X | 1 | Lymphoma Malignant Mixed | X | 1 | Thecoma Benign | | 1 | __________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + | 70 | Hemangioma | | 1 | Polyp Stromal | X | 4 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 11 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 4| 7| 7| 2| 2| 7| 2| 6| 7| 4| 2| 2| 7| 7| 4| 6| | | DAY ON TEST | 3| 2| 3| 3| 5| 3| 3| 8| 8| 3| 8| 4| 3| 0| 8| 8| 3| 0| 8| 5| | | | 7| 3| 7| 7| 7| 7| 7| 2| 2| 7| 2| 4| 7| 1| 2| 2| 7| 8| 5| 8| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | CONTROL | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + | 70 | Hemangiosarcoma, Metastatic, Spleen | | 1 | Histiocytic Sarcoma | X | 1 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + + + + + | 70 | Lymphoma Malignant Lymphocytic | X | 1 | Lymphoma Malignant Mixed | X | 1 | Iliac, Lymphoma Malignant Lymphocytic| X | 1 | Iliac, Lymphoma Malignant Mixed | | 1 | Pancreatic, Lymphoma Malignant Mixed | X | 1 | Renal, Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________|____________| Lymph Node, Bronchial | + + M + + + + + + + + + + M M + + + + + | 58 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X | 1 | Lymphoma Malignant Mixed | X | 1 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + M + + + I M M + M M + + + + + | 53 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Mixed | X X | 2 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | M + M M + M + M M M + + + + + + M + + + | 39 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Mixed | X | 1 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + | 69 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X | 1 | Lymphoma Malignant Mixed | X | 4 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + | 69 | Hemangiosarcoma | | 2 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X | 2 | Lymphoma Malignant Mixed | X X | 4 | __________________________________________________________________________|____________| Thymus | + A + + + + + + + + + M + + + + + + + + | 66 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Mixed | X | 1 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + + + + M + | 69 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + | 70 | Subcutaneous Tissue, Hemangiosarcoma | | 1 | Subcutaneous Tissue, Sarcoma | | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 12 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 4| 7| 7| 2| 2| 7| 2| 6| 7| 4| 2| 2| 7| 7| 4| 6| | | DAY ON TEST | 3| 2| 3| 3| 5| 3| 3| 8| 8| 3| 8| 4| 3| 0| 8| 8| 3| 0| 8| 5| | | | 7| 3| 7| 7| 7| 7| 7| 2| 2| 7| 2| 4| 7| 1| 2| 2| 7| 8| 5| 8| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | CONTROL | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ MUSCULOSKELETAL SYSTEM - cont | | | | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Skeletal Muscle | | 1 | Hemangiosarcoma, Metastatic, Spleen | | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + | 70 | Lymphoma Malignant Lymphocytic | X | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + | 70 | Alveolar/Bronchiolar Adenoma | X | 4 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X | 1 | Mediastinum, Sarcoma, Metastatic, | | | Skin | | 1 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + | 69 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | + | 3 | __________________________________________________________________________|____________| Harderian Gland | + + + + + + + + I + + + + + + M + + + + | 64 | Adenoma | X X | 10 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + | 69 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X | 1 | Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + | 67 | Lymphoma Malignant Lymphocytic | X | 1 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + | 70 | Histiocytic Sarcoma | X | 3 | Lymphoma Malignant Lymphocytic | X | 2 | Lymphoma Malignant Mixed | X X | 5 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 13 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 7| 7| 5| 2| 7| 7| 7| 4| 7| 7| 4| 7| 7| 7| 2| 7| 7| 7| 5| 7| 4| 2| 0| 7| DAY ON TEST | 5| 3| 3| 7| 8| 3| 2| 3| 5| 3| 3| 5| 3| 3| 3| 8| 3| 3| 3| 2| 3| 5| 8| 0| 3| | 7| 5| 5| 2| 2| 5| 2| 5| 7| 5| 5| 7| 5| 2| 5| 2| 5| 5| 5| 4| 5| 7| 2| 7| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 6.25 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + M + + + + M + + + + + + M + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + A + A A| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + A +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + A + A +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + A + A +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + M +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + A + A +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + A + A A| Adenoma | | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + A + M +| Carcinoma | X | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + A + A +| Carcinoma | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + A +| Hepatocellular Carcinoma | | Hepatocellular Carcinoma, Multiple | | Hepatocellular Adenoma | X X | Hepatocellular Adenoma, Multiple | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X X | __________________________________________________________________________| Mesentery | + + + + | Fat, Lymphoma Malignant Lymphocytic | | Fat, Sarcoma, Metastatic, Uterus | X | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + A +| Acinus, Lymphoma Malignant | | Lymphocytic | | Acinus, Lymphoma Malignant Mixed | X X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + M + +| Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | | __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + A +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + A +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + A +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Tooth | + + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Blood Vessel | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Lung | | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| Page 14 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 7| 7| 5| 2| 7| 7| 7| 4| 7| 7| 4| 7| 7| 7| 2| 7| 7| 7| 5| 7| 4| 2| 0| 7| DAY ON TEST | 5| 3| 3| 7| 8| 3| 2| 3| 5| 3| 3| 5| 3| 3| 3| 8| 3| 3| 3| 2| 3| 5| 8| 0| 3| | 7| 5| 5| 2| 2| 5| 2| 5| 7| 5| 5| 7| 5| 2| 5| 2| 5| 5| 5| 4| 5| 7| 2| 7| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 6.25 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + A +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + A +| Hepatocellular Carcinoma, Metastatic,| | Liver | | Histiocytic Sarcoma | | Lymphoma Malignant Mixed | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + A I| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + A +| __________________________________________________________________________| Parathyroid Gland | + M + + M + + + + M M + + + + + M M| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + A +| Pars Distalis, Adenoma | X X X X X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + M +| Lymphoma Malignant Lymphocytic | | Follicular Cell, Adenoma | | Follicular Cell, Carcinoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + A +| Adenoma | X | Granular Cell Tumor Benign | | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + A +| Adenocarcinoma | | Hemangioma | X | Leiomyosarcoma | | Lymphoma Malignant Lymphocytic | | Polyp Stromal | X X| Sarcoma | X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + A +| Hemangioma | X | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + +| Iliac, Lymphoma Malignant Lymphocytic| | Iliac, Lymphoma Malignant Mixed | X | _____________________________________________________________________________________________________________________| Page 15 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 7| 7| 5| 2| 7| 7| 7| 4| 7| 7| 4| 7| 7| 7| 2| 7| 7| 7| 5| 7| 4| 2| 0| 7| DAY ON TEST | 5| 3| 3| 7| 8| 3| 2| 3| 5| 3| 3| 5| 3| 3| 3| 8| 3| 3| 3| 2| 3| 5| 8| 0| 3| | 7| 5| 5| 2| 2| 5| 2| 5| 7| 5| 5| 7| 5| 2| 5| 2| 5| 5| 5| 4| 5| 7| 2| 7| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 6.25 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Pancreatic, Lymphoma Malignant Mixed | X | Renal, Histiocytic Sarcoma | | Renal, Lymphoma Malignant Lymphocytic| | Renal, Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node, Bronchial | + + + + M + + M + + + + + + + + M +| Adenocarcinoma, Metastatic, Lung | | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + M M + + + + M + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Lymph Node, Mediastinal | + M + M M + + M + + M + M + + + M +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + I + + + + + + + + M +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X X X X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + A +| Hemangioma | X | Hemangiosarcoma | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X X X | __________________________________________________________________________| Thymus | + + + + + + + + + + M + + + + + + +| Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M + + + + + M + + + + + + + + + + +| Adenoacanthoma | X | Adenocarcinoma | X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Hemangiosarcoma | X | Subcutaneous Tissue, Sarcoma | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, | | Harderian Gland | X | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | _____________________________________________________________________________________________________________________| Page 16 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 7| 7| 5| 2| 7| 7| 7| 4| 7| 7| 4| 7| 7| 7| 2| 7| 7| 7| 5| 7| 4| 2| 0| 7| DAY ON TEST | 5| 3| 3| 7| 8| 3| 2| 3| 5| 3| 3| 5| 3| 3| 3| 8| 3| 3| 3| 2| 3| 5| 8| 0| 3| | 7| 5| 5| 2| 2| 5| 2| 5| 7| 5| 5| 7| 5| 2| 5| 2| 5| 5| 5| 4| 5| 7| 2| 7| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 6.25 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM - cont | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | | Adenocarcinoma, Multiple | | Adenocarcinoma, Metastatic, | | Harderian Gland | X | Adenocarcinoma, Metastatic, Mammary | | Gland | | Adenocarcinoma, Metastatic, Uterus | | Alveolar/Bronchiolar Adenoma | X X X X | Alveolar/Bronchiolar Carcinoma | X | Hepatocellular Carcinoma, Metastatic,| | Liver | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | | Mediastinum, Adenocarcinoma, | | Metastatic, Lung | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + A +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + M +| Adenocarcinoma | X | Adenoma | X X X X X X | Bilateral, Adenoma | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + A +| Adenocarcinoma, Metastatic, Lung | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + M + + + A +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| Page 17 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 7| 7| 5| 2| 7| 7| 7| 4| 7| 7| 4| 7| 7| 7| 2| 7| 7| 7| 5| 7| 4| 2| 0| 7| DAY ON TEST | 5| 3| 3| 7| 8| 3| 2| 3| 5| 3| 3| 5| 3| 3| 3| 8| 3| 3| 3| 2| 3| 5| 8| 0| 3| | 7| 5| 5| 2| 2| 5| 2| 5| 7| 5| 5| 7| 5| 2| 5| 2| 5| 5| 5| 4| 5| 7| 2| 7| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 6.25 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS - cont | | | | Lymphoma Malignant Mixed | X X X X X | _____________________________________________________________________________________________________________________| Page 18 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 5| 7| 7| 4| 7| 4| 2| 2| 6| 7| 2| 7| 7| 6| 7| 7| 5| 7| 4| 7| 4| 4| 7| 7| 6| DAY ON TEST | 1| 3| 3| 5| 3| 6| 8| 8| 5| 3| 8| 3| 3| 4| 3| 3| 3| 3| 5| 3| 5| 5| 3| 3| 9| | 9| 5| 5| 7| 5| 7| 2| 2| 3| 5| 2| 5| 5| 2| 6| 6| 8| 6| 7| 6| 7| 7| 6| 6| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6.25 PPM | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + M + + + + + + + + + M M +| __________________________________________________________________________| Gallbladder | + + + + A + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + A + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + M +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + I + + + + + + + + + + + +| Adenoma | X | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + +| Carcinoma | | Lymphoma Malignant Mixed | | __________________________________________________________________________| Intestine Small, Jejunum | + + + + A + + + + + + + + + + + + +| Carcinoma | X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Carcinoma | X X | Hepatocellular Carcinoma, Multiple | X| Hepatocellular Adenoma | X X X X X | Hepatocellular Adenoma, Multiple | X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | X X | __________________________________________________________________________| Mesentery | + + + +| Fat, Lymphoma Malignant Lymphocytic | X | Fat, Sarcoma, Metastatic, Uterus | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + +| Acinus, Lymphoma Malignant | | Lymphocytic | | Acinus, Lymphoma Malignant Mixed | X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Tooth | + + + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Blood Vessel | + | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Lung | X| Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| Page 19 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 5| 7| 7| 4| 7| 4| 2| 2| 6| 7| 2| 7| 7| 6| 7| 7| 5| 7| 4| 7| 4| 4| 7| 7| 6| DAY ON TEST | 1| 3| 3| 5| 3| 6| 8| 8| 5| 3| 8| 3| 3| 4| 3| 3| 3| 3| 5| 3| 5| 5| 3| 3| 9| | 9| 5| 5| 7| 5| 7| 2| 2| 3| 5| 2| 5| 5| 2| 6| 6| 8| 6| 7| 6| 7| 7| 6| 6| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6.25 PPM | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + +| Hepatocellular Carcinoma, Metastatic,| | Liver | X| Histiocytic Sarcoma | X | Lymphoma Malignant Mixed | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + +| Pars Distalis, Adenoma | X X X X X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | Follicular Cell, Adenoma | | Follicular Cell, Carcinoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | M | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Granular Cell Tumor Benign | X | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X | Hemangioma | | Leiomyosarcoma | | Lymphoma Malignant Lymphocytic | X | Polyp Stromal | | Sarcoma | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + +| Hemangioma | | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + +| Iliac, Lymphoma Malignant Lymphocytic| | Iliac, Lymphoma Malignant Mixed | X | _____________________________________________________________________________________________________________________| Page 20 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 5| 7| 7| 4| 7| 4| 2| 2| 6| 7| 2| 7| 7| 6| 7| 7| 5| 7| 4| 7| 4| 4| 7| 7| 6| DAY ON TEST | 1| 3| 3| 5| 3| 6| 8| 8| 5| 3| 8| 3| 3| 4| 3| 3| 3| 3| 5| 3| 5| 5| 3| 3| 9| | 9| 5| 5| 7| 5| 7| 2| 2| 3| 5| 2| 5| 5| 2| 6| 6| 8| 6| 7| 6| 7| 7| 6| 6| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6.25 PPM | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Pancreatic, Lymphoma Malignant Mixed | | Renal, Histiocytic Sarcoma | | Renal, Lymphoma Malignant Lymphocytic| | Renal, Lymphoma Malignant Mixed | X | __________________________________________________________________________| Lymph Node, Bronchial | + + + + M + + + + + I + + + + + + +| Adenocarcinoma, Metastatic, Lung | X| Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + M M + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Lymph Node, Mediastinal | + + M M + + M M M + M + + M M + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + I + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + +| Hemangioma | | Hemangiosarcoma | X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | X X X | __________________________________________________________________________| Thymus | + + + + M + + + + + + + + + + + + M| Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + M + + + +| Adenoacanthoma | | Adenocarcinoma | | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Hemangiosarcoma | X | Subcutaneous Tissue, Sarcoma | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, | | Harderian Gland | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | _____________________________________________________________________________________________________________________| Page 21 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 5| 7| 7| 4| 7| 4| 2| 2| 6| 7| 2| 7| 7| 6| 7| 7| 5| 7| 4| 7| 4| 4| 7| 7| 6| DAY ON TEST | 1| 3| 3| 5| 3| 6| 8| 8| 5| 3| 8| 3| 3| 4| 3| 3| 3| 3| 5| 3| 5| 5| 3| 3| 9| | 9| 5| 5| 7| 5| 7| 2| 2| 3| 5| 2| 5| 5| 2| 6| 6| 8| 6| 7| 6| 7| 7| 6| 6| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6.25 PPM | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM - cont | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X | Adenocarcinoma, Multiple | X| Adenocarcinoma, Metastatic, | | Harderian Gland | | Adenocarcinoma, Metastatic, Mammary | | Gland | | Adenocarcinoma, Metastatic, Uterus | X | Alveolar/Bronchiolar Adenoma | X X X X | Alveolar/Bronchiolar Carcinoma | X | Hepatocellular Carcinoma, Metastatic,| | Liver | X| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | X | Mediastinum, Adenocarcinoma, | | Metastatic, Lung | X| __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + +| Adenocarcinoma | | Adenoma | X X | Bilateral, Adenoma | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Lung | X| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | X X | __________________________________________________________________________| Urinary Bladder | + + M + M + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| Page 22 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 5| 7| 7| 4| 7| 4| 2| 2| 6| 7| 2| 7| 7| 6| 7| 7| 5| 7| 4| 7| 4| 4| 7| 7| 6| DAY ON TEST | 1| 3| 3| 5| 3| 6| 8| 8| 5| 3| 8| 3| 3| 4| 3| 3| 3| 3| 5| 3| 5| 5| 3| 3| 9| | 9| 5| 5| 7| 5| 7| 2| 2| 3| 5| 2| 5| 5| 2| 6| 6| 8| 6| 7| 6| 7| 7| 6| 6| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6.25 PPM | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS - cont | | | | Lymphoma Malignant Mixed | X X X | _____________________________________________________________________________________________________________________| Page 23 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 4| 7| 7| 2| 6| 6| 7| 7| 2| 7| 7| 6| 6| 7| 4| 7| 2| 2| 7| 7| | | DAY ON TEST | 5| 3| 3| 8| 9| 7| 3| 3| 8| 3| 3| 4| 4| 3| 5| 3| 8| 8| 3| 3| | | | 7| 6| 6| 2| 5| 3| 6| 6| 2| 0| 6| 4| 1| 6| 7| 6| 2| 2| 6| 6| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | A | 6.25 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + M + + + + + M + + M | 41 | __________________________________________________________________________|____________| Gallbladder | + + + + + + A + + M + + + + | 44 | __________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + A + + + + | 46 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + | 48 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + A + + + + | 47 | __________________________________________________________________________|____________| Intestine Small | + + + + + + + + + A + + + + | 47 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + A + + + + | 45 | Adenoma | | 1 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + A + + A + + + + | 46 | Carcinoma | | 1 | Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + A + + A + + + + | 45 | Carcinoma | | 1 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + | 52 | Hepatocellular Carcinoma | X X X | 5 | Hepatocellular Carcinoma, Multiple | | 1 | Hepatocellular Adenoma | X X X | 10 | Hepatocellular Adenoma, Multiple | | 1 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | | 1 | Lymphoma Malignant Mixed | X | 5 | __________________________________________________________________________|____________| Mesentery | + + + + + | 13 | Fat, Lymphoma Malignant Lymphocytic | X X | 3 | Fat, Sarcoma, Metastatic, Uterus | | 1 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + | 49 | Acinus, Lymphoma Malignant | | | Lymphocytic | X | 1 | Acinus, Lymphoma Malignant Mixed | | 3 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + | 49 | Lymphoma Malignant Lymphocytic | X | 1 | Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + | 50 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + A + + + + | 49 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Tooth | | 5 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 24 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 4| 7| 7| 2| 6| 6| 7| 7| 2| 7| 7| 6| 6| 7| 4| 7| 2| 2| 7| 7| | | DAY ON TEST | 5| 3| 3| 8| 9| 7| 3| 3| 8| 3| 3| 4| 4| 3| 5| 3| 8| 8| 3| 3| | | | 7| 6| 6| 2| 5| 3| 6| 6| 2| 0| 6| 4| 1| 6| 7| 6| 2| 2| 6| 6| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | A | 6.25 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ CARDIOVASCULAR SYSTEM - cont | | | | | | | | | __________________________________________________________________________|____________| Blood Vessel | | 1 | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + | 50 | Adenocarcinoma, Metastatic, Lung | | 1 | Lymphoma Malignant Lymphocytic | | 1 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + | 49 | Hepatocellular Carcinoma, Metastatic,| | | Liver | | 1 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Mixed | X | 1 | __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + | 48 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Parathyroid Gland | + + M + + + M + M + + + + M | 40 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + | 49 | Pars Distalis, Adenoma | X X | 12 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + | 49 | Lymphoma Malignant Lymphocytic | X | 1 | Follicular Cell, Adenoma | X | 1 | Follicular Cell, Carcinoma | | 1 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Clitoral Gland | | | __________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + | 59 | Adenoma | X X | 4 | Granular Cell Tumor Benign | | 1 | Lymphoma Malignant Lymphocytic | X | 1 | Lymphoma Malignant Mixed | X | 2 | __________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + | 50 | Adenocarcinoma | | 1 | Hemangioma | | 1 | Leiomyosarcoma | X | 1 | Lymphoma Malignant Lymphocytic | | 1 | Polyp Stromal | | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 25 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 4| 7| 7| 2| 6| 6| 7| 7| 2| 7| 7| 6| 6| 7| 4| 7| 2| 2| 7| 7| | | DAY ON TEST | 5| 3| 3| 8| 9| 7| 3| 3| 8| 3| 3| 4| 4| 3| 5| 3| 8| 8| 3| 3| | | | 7| 6| 6| 2| 5| 3| 6| 6| 2| 0| 6| 4| 1| 6| 7| 6| 2| 2| 6| 6| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | A | 6.25 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ GENITAL SYSTEM - cont | | | | | | Sarcoma | | 1 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Blood | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + | 49 | Hemangioma | | 1 | Lymphoma Malignant Lymphocytic | X | 1 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + | 50 | Iliac, Lymphoma Malignant Lymphocytic| X | 1 | Iliac, Lymphoma Malignant Mixed | | 2 | Pancreatic, Lymphoma Malignant Mixed | | 1 | Renal, Histiocytic Sarcoma | X | 1 | Renal, Lymphoma Malignant Lymphocytic| X | 1 | Renal, Lymphoma Malignant Mixed | X | 2 | __________________________________________________________________________|____________| Lymph Node, Bronchial | M + + M + + + + + + M + + + | 42 | Adenocarcinoma, Metastatic, Lung | | 1 | Lymphoma Malignant Lymphocytic | X | 2 | Lymphoma Malignant Mixed | X | 2 | __________________________________________________________________________|____________| Lymph Node, Mandibular | M M + + + + + + M + + + + + | 42 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant Lymphocytic | X X | 3 | Lymphoma Malignant Mixed | X | 3 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | M + + + M + + M M + M + + M | 29 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant Lymphocytic | X | 1 | Lymphoma Malignant Mixed | X | 4 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + M + + + + | 46 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X | 1 | Lymphoma Malignant Mixed | X | 6 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + | 49 | Hemangioma | | 1 | Hemangiosarcoma | | 1 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X X | 3 | Lymphoma Malignant Mixed | X | 7 | __________________________________________________________________________|____________| Thymus | + + + + + + + M + + + + + + | 46 | Lymphoma Malignant Lymphocytic | X | 2 | Lymphoma Malignant Mixed | X | 4 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 26 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 4| 7| 7| 2| 6| 6| 7| 7| 2| 7| 7| 6| 6| 7| 4| 7| 2| 2| 7| 7| | | DAY ON TEST | 5| 3| 3| 8| 9| 7| 3| 3| 8| 3| 3| 4| 4| 3| 5| 3| 8| 8| 3| 3| | | | 7| 6| 6| 2| 5| 3| 6| 6| 2| 0| 6| 4| 1| 6| 7| 6| 2| 2| 6| 6| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | A | 6.25 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | + + + + + + M + + + + + + + | 46 | Adenoacanthoma | | 1 | Adenocarcinoma | X | 2 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + | 50 | Subcutaneous Tissue, Hemangiosarcoma | | 2 | Subcutaneous Tissue, Sarcoma | X | 2 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + | 50 | Adenocarcinoma, Metastatic, | | | Harderian Gland | | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + | 50 | Histiocytic Sarcoma | | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + | 60 | Adenocarcinoma | | 1 | Adenocarcinoma, Multiple | | 1 | Adenocarcinoma, Metastatic, | | | Harderian Gland | | 1 | Adenocarcinoma, Metastatic, Mammary | | | Gland | X | 1 | Adenocarcinoma, Metastatic, Uterus | | 1 | Alveolar/Bronchiolar Adenoma | X X X | 11 | Alveolar/Bronchiolar Carcinoma | X | 3 | Hepatocellular Carcinoma, Metastatic,| | | Liver | | 1 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X X | 3 | Lymphoma Malignant Mixed | X | 2 | Mediastinum, Adenocarcinoma, | | | Metastatic, Lung | | 1 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 27 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 4| 7| 7| 2| 6| 6| 7| 7| 2| 7| 7| 6| 6| 7| 4| 7| 2| 2| 7| 7| | | DAY ON TEST | 5| 3| 3| 8| 9| 7| 3| 3| 8| 3| 3| 4| 4| 3| 5| 3| 8| 8| 3| 3| | | | 7| 6| 6| 2| 5| 3| 6| 6| 2| 0| 6| 4| 1| 6| 7| 6| 2| 2| 6| 6| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | A | 6.25 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - cont | | | | | | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + | 50 | Adenocarcinoma | | 1 | Adenoma | X X | 10 | Bilateral, Adenoma | | 1 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + | 49 | Adenocarcinoma, Metastatic, Lung | | 1 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X | 2 | Lymphoma Malignant Mixed | X | 4 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + | 46 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant Lymphocytic | X | 2 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + | 60 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X X | 3 | Lymphoma Malignant Mixed | X | 9 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 28 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 6| 1| 4| 4| 6| 7| 2| 6| 7| 4| 2| 2| 6| 4| 5| 7| 7| 6| 5| 4| 7| 6| 7| 6| DAY ON TEST | 3| 4| 9| 5| 5| 7| 3| 8| 8| 3| 5| 8| 8| 9| 5| 0| 3| 3| 5| 3| 5| 3| 9| 3| 5| | 4| 5| 4| 7| 7| 7| 4| 2| 1| 4| 7| 2| 2| 7| 7| 7| 4| 4| 0| 3| 7| 4| 1| 4| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 20 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + M + + + + + + +| __________________________________________________________________________| Gallbladder | + + + A + + + + A + + A + + A + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Intestine Large | + + + A + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + A + + + + A + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + A + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + A + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + A + + + + A + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + A + + + + A + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + A + + + + A + + + + + A + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + A + + + + A + + + + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Parathyroid | | Gland | | Hepatocellular Carcinoma | X X X| Hepatocellular Carcinoma, Multiple | | Hepatocellular Adenoma | X | Hepatocellular Adenoma, Multiple | | Histiocytic Sarcoma | X X X | Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant Mixed | X | Lymphoma Malignant Undifferentiated | | Cell Type | | __________________________________________________________________________| Mesentery | + + + + | Fat, Histiocytic Sarcoma | | Fat, Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Pancreas | + + + A + + + + + + + + + + + + +| Acinus, Lymphoma Malignant | | Lymphocytic | X X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + A + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + A + + + + + + + + + + + + + + + +| Squamous Cell Carcinoma | | Squamous Cell Papilloma | | __________________________________________________________________________| Stomach, Glandular | + + + + + A + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Tooth | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | Pericardium, Leiomyosarcoma, | | _____________________________________________________________________________________________________________________| Page 29 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 6| 1| 4| 4| 6| 7| 2| 6| 7| 4| 2| 2| 6| 4| 5| 7| 7| 6| 5| 4| 7| 6| 7| 6| DAY ON TEST | 3| 4| 9| 5| 5| 7| 3| 8| 8| 3| 5| 8| 8| 9| 5| 0| 3| 3| 5| 3| 5| 3| 9| 3| 5| | 4| 5| 4| 7| 7| 7| 4| 2| 1| 4| 7| 2| 2| 7| 7| 7| 4| 4| 0| 3| 7| 4| 1| 4| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 20 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | | | Metastatic, Uterus | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + A + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + A + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Adrenal Gland, Medulla | + + + A + + + + + + + + + + + + +| Pheochromocytoma Malignant | | Pheochromocytoma Benign | X | __________________________________________________________________________| Islets, Pancreatic | + + M A + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | M + M + + M + M M + + + + + M M M| Carcinoma | | __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + +| Pars Distalis, Adenoma | X X X | __________________________________________________________________________| Thyroid Gland | + + + A + + + + + + + + + + + + +| Carcinoma, Metastatic, Parathyroid | | Gland | | Follicular Cell, Adenoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | Granulosa Cell Tumor Benign | X | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Leiomyosarcoma | | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + A + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + +| Axillary, Lymphoma Malignant | | Lymphocytic | | Iliac, Histiocytic Sarcoma | X | Iliac, Lymphoma Malignant Lymphocytic| | _____________________________________________________________________________________________________________________| Page 30 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 6| 1| 4| 4| 6| 7| 2| 6| 7| 4| 2| 2| 6| 4| 5| 7| 7| 6| 5| 4| 7| 6| 7| 6| DAY ON TEST | 3| 4| 9| 5| 5| 7| 3| 8| 8| 3| 5| 8| 8| 9| 5| 0| 3| 3| 5| 3| 5| 3| 9| 3| 5| | 4| 5| 4| 7| 7| 7| 4| 2| 1| 4| 7| 2| 2| 7| 7| 7| 4| 4| 0| 3| 7| 4| 1| 4| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 20 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Renal, Histiocytic Sarcoma | X X | Renal, Lymphoma Malignant Lymphocytic| | Renal, Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node, Bronchial | + M + M + M + + + + + + M M + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + M M +| Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X | Sarcoma, Metastatic, Skin | X | __________________________________________________________________________| Lymph Node, Mediastinal | M M + A + + + M + M + M M M + + +| Hepatocellular Carcinoma, Metastatic,| | Liver | X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + A + + + + + + + + + + + + +| Histiocytic Sarcoma | X X X | Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Spleen | + + + A + + + + + + + + + + + + +| Histiocytic Sarcoma | X X X | Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant Mixed | X | Lymphoma Malignant Undifferentiated | | Cell Type | | __________________________________________________________________________| Thymus | + + + M + M + + + + + + + + + M +| Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant Mixed | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + +| Adenoacanthoma | | Adenocarcinoma | X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Hemangioma | | Subcutaneous Tissue, Hemangiosarcoma | X | Subcutaneous Tissue, | | Neurofibrosarcoma | | Subcutaneous Tissue, Sarcoma | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | + | _____________________________________________________________________________________________________________________| Page 31 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 6| 1| 4| 4| 6| 7| 2| 6| 7| 4| 2| 2| 6| 4| 5| 7| 7| 6| 5| 4| 7| 6| 7| 6| DAY ON TEST | 3| 4| 9| 5| 5| 7| 3| 8| 8| 3| 5| 8| 8| 9| 5| 0| 3| 3| 5| 3| 5| 3| 9| 3| 5| | 4| 5| 4| 7| 7| 7| 4| 2| 1| 4| 7| 2| 2| 7| 7| 7| 4| 4| 0| 3| 7| 4| 1| 4| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 20 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM - cont | | | | Adenocarcinoma, Metastatic, Lung | X | Hepatocellular Carcinoma, Metastatic,| | Liver | X | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + A + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X X | Adenocarcinoma, Metastatic, | | Harderian Gland | | Alveolar/Bronchiolar Adenoma | X X X X| Alveolar/Bronchiolar Adenoma, | | Multiple | | Alveolar/Bronchiolar Carcinoma | X X X X | Carcinoma, Metastatic, Parathyroid | | Gland | | Hepatocellular Carcinoma, Metastatic,| | Liver | X X X| Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X | Pheochromocytoma Malignant, | | Metastatic, Adrenal Gland | | Fat, Mediastinum, Hemangioma | | Mediastinum, Adenocarcinoma, | | Metastatic, Lung | X X | Mediastinum, Hemangiosarcoma, | | Metastatic, Skin | | Mediastinum, Hepatocellular | | Carcinoma, Metastatic, Liver | X | Mediastinum, Leiomyosarcoma, | | Metastatic, Uterus | | __________________________________________________________________________| Nose | + + + A + + + + A + + + + + + + +| __________________________________________________________________________| Trachea | + + + A + + + + A + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | A | __________________________________________________________________________| Eye | M | __________________________________________________________________________| Harderian Gland | + + M + + + + + + + + + + + + + +| Adenocarcinoma | | Adenoma | | _____________________________________________________________________________________________________________________| Page 32 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 6| 1| 4| 4| 6| 7| 2| 6| 7| 4| 2| 2| 6| 4| 5| 7| 7| 6| 5| 4| 7| 6| 7| 6| DAY ON TEST | 3| 4| 9| 5| 5| 7| 3| 8| 8| 3| 5| 8| 8| 9| 5| 0| 3| 3| 5| 3| 5| 3| 9| 3| 5| | 4| 5| 4| 7| 7| 7| 4| 2| 1| 4| 7| 2| 2| 7| 7| 7| 4| 4| 0| 3| 7| 4| 1| 4| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 20 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + A + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Lung | X | Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | X | Carcinoma, Metastatic, Parathyroid | | Gland | | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Urinary Bladder | + + + A + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X X X | Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant Mixed | X | Lymphoma Malignant Undifferentiated | | Cell Type | | _____________________________________________________________________________________________________________________| Page 33 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 6| 4| 5| 7| 4| 7| 7| 2| 4| 5| 5| 5| 4| 3| 2| 2| 2| 6| 2| 7| 6| 7| 6| 7| DAY ON TEST | 3| 5| 5| 6| 3| 5| 3| 3| 8| 5| 4| 7| 4| 9| 6| 8| 8| 8| 0| 8| 3| 8| 3| 7| 3| | 4| 7| 7| 9| 4| 7| 4| 4| 2| 7| 1| 3| 1| 2| 5| 2| 2| 2| 0| 2| 4| 1| 4| 7| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 20 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + A A + + + + + A +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + A + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + A + + + + A +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + A + + + + A +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + A A + + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Parathyroid | | Gland | X | Hepatocellular Carcinoma | X | Hepatocellular Carcinoma, Multiple | X | Hepatocellular Adenoma | X X X | Hepatocellular Adenoma, Multiple | | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | | Lymphoma Malignant Undifferentiated | | Cell Type | | __________________________________________________________________________| Mesentery | + + + + +| Fat, Histiocytic Sarcoma | X | Fat, Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + +| Acinus, Lymphoma Malignant | | Lymphocytic | X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + +| Squamous Cell Carcinoma | | Squamous Cell Papilloma | X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Tooth | + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Pericardium, Leiomyosarcoma, | | _____________________________________________________________________________________________________________________| Page 34 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 6| 4| 5| 7| 4| 7| 7| 2| 4| 5| 5| 5| 4| 3| 2| 2| 2| 6| 2| 7| 6| 7| 6| 7| DAY ON TEST | 3| 5| 5| 6| 3| 5| 3| 3| 8| 5| 4| 7| 4| 9| 6| 8| 8| 8| 0| 8| 3| 8| 3| 7| 3| | 4| 7| 7| 9| 4| 7| 4| 4| 2| 7| 1| 3| 1| 2| 5| 2| 2| 2| 0| 2| 4| 1| 4| 7| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 20 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | | | Metastatic, Uterus | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + +| Pheochromocytoma Malignant | | Pheochromocytoma Benign | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | + + M + + + M + + + + M + + + M +| Carcinoma | X | __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + +| Pars Distalis, Adenoma | X X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Parathyroid | | Gland | X | Follicular Cell, Adenoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + +| Adenoma | | Granulosa Cell Tumor Benign | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Leiomyosarcoma | | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + +| Axillary, Lymphoma Malignant | | Lymphocytic | X | Iliac, Histiocytic Sarcoma | | Iliac, Lymphoma Malignant Lymphocytic| X | _____________________________________________________________________________________________________________________| Page 35 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 6| 4| 5| 7| 4| 7| 7| 2| 4| 5| 5| 5| 4| 3| 2| 2| 2| 6| 2| 7| 6| 7| 6| 7| DAY ON TEST | 3| 5| 5| 6| 3| 5| 3| 3| 8| 5| 4| 7| 4| 9| 6| 8| 8| 8| 0| 8| 3| 8| 3| 7| 3| | 4| 7| 7| 9| 4| 7| 4| 4| 2| 7| 1| 3| 1| 2| 5| 2| 2| 2| 0| 2| 4| 1| 4| 7| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 20 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Renal, Histiocytic Sarcoma | | Renal, Lymphoma Malignant Lymphocytic| | Renal, Lymphoma Malignant Mixed | X | __________________________________________________________________________| Lymph Node, Bronchial | + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + M + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | Sarcoma, Metastatic, Skin | | __________________________________________________________________________| Lymph Node, Mediastinal | + + + M + + + + + + M + + + + + +| Hepatocellular Carcinoma, Metastatic,| | Liver | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | X | Lymphoma Malignant Undifferentiated | | Cell Type | | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant Mixed | X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + +| Adenoacanthoma | X | Adenocarcinoma | X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Hemangioma | | Subcutaneous Tissue, Hemangiosarcoma | X | Subcutaneous Tissue, | | Neurofibrosarcoma | X | Subcutaneous Tissue, Sarcoma | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | | _____________________________________________________________________________________________________________________| Page 36 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 6| 4| 5| 7| 4| 7| 7| 2| 4| 5| 5| 5| 4| 3| 2| 2| 2| 6| 2| 7| 6| 7| 6| 7| DAY ON TEST | 3| 5| 5| 6| 3| 5| 3| 3| 8| 5| 4| 7| 4| 9| 6| 8| 8| 8| 0| 8| 3| 8| 3| 7| 3| | 4| 7| 7| 9| 4| 7| 4| 4| 2| 7| 1| 3| 1| 2| 5| 2| 2| 2| 0| 2| 4| 1| 4| 7| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 20 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM - cont | | | | Adenocarcinoma, Metastatic, Lung | | Hepatocellular Carcinoma, Metastatic,| | Liver | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | | Adenocarcinoma, Metastatic, | | Harderian Gland | X | Alveolar/Bronchiolar Adenoma | X X X | Alveolar/Bronchiolar Adenoma, | | Multiple | X X | Alveolar/Bronchiolar Carcinoma | X | Carcinoma, Metastatic, Parathyroid | | Gland | X | Hepatocellular Carcinoma, Metastatic,| | Liver | X | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X | Pheochromocytoma Malignant, | | Metastatic, Adrenal Gland | | Fat, Mediastinum, Hemangioma | | Mediastinum, Adenocarcinoma, | | Metastatic, Lung | | Mediastinum, Hemangiosarcoma, | | Metastatic, Skin | X | Mediastinum, Hepatocellular | | Carcinoma, Metastatic, Liver | | Mediastinum, Leiomyosarcoma, | | Metastatic, Uterus | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | | __________________________________________________________________________| Eye | + + | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X | Adenoma | X X X X X X | _____________________________________________________________________________________________________________________| Page 37 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 6| 4| 5| 7| 4| 7| 7| 2| 4| 5| 5| 5| 4| 3| 2| 2| 2| 6| 2| 7| 6| 7| 6| 7| DAY ON TEST | 3| 5| 5| 6| 3| 5| 3| 3| 8| 5| 4| 7| 4| 9| 6| 8| 8| 8| 0| 8| 3| 8| 3| 7| 3| | 4| 7| 7| 9| 4| 7| 4| 4| 2| 7| 1| 3| 1| 2| 5| 2| 2| 2| 0| 2| 4| 1| 4| 7| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 20 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Lung | | Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | | Carcinoma, Metastatic, Parathyroid | | Gland | X | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant Mixed | X | Lymphoma Malignant Undifferentiated | | Cell Type | | _____________________________________________________________________________________________________________________| Page 38 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 6| 7| 7| 6| 2| 7| 7| 7| 6| 7| 6| 4| 7| 6| 7| 2| 7| 2| 7| 4| | | DAY ON TEST | 5| 3| 3| 8| 8| 3| 3| 3| 2| 3| 4| 5| 3| 0| 3| 7| 3| 8| 3| 5| | | | 3| 4| 4| 6| 2| 4| 4| 4| 5| 4| 9| 7| 4| 4| 4| 2| 4| 2| 4| 7| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | A | 20 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + M + M + + + | 47 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + + + A + + + A + + | 41 | Lymphoma Malignant Lymphocytic | X | 1 | __________________________________________________________________________|____________| Intestine Large | + + + + + + + + + A + + + A + + | 47 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + A + + + A + + | 46 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + A + + + A + + | 47 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + A + + + A + + | 47 | __________________________________________________________________________|____________| Intestine Small | + + + + + + + + + A + + + A + + | 45 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + A + + + A + + | 44 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + A + + + A + + | 43 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + A + + + A + + | 44 | Lymphoma Malignant Lymphocytic | X | 1 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + | 51 | Carcinoma, Metastatic, Parathyroid | | | Gland | | 1 | Hepatocellular Carcinoma | X X X | 7 | Hepatocellular Carcinoma, Multiple | | 1 | Hepatocellular Adenoma | X X X X | 8 | Hepatocellular Adenoma, Multiple | X | 1 | Histiocytic Sarcoma | X | 6 | Lymphoma Malignant Lymphocytic | X | 4 | Lymphoma Malignant Mixed | | 1 | Lymphoma Malignant Undifferentiated | | | Cell Type | X | 1 | __________________________________________________________________________|____________| Mesentery | + + + + + + + | 16 | Fat, Histiocytic Sarcoma | X | 2 | Fat, Lymphoma Malignant Lymphocytic | X | 2 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + A + + + A + + | 47 | Acinus, Lymphoma Malignant | | | Lymphocytic | | 3 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + A + + + + + + + + | 58 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + A + + + + M + + + | 57 | Squamous Cell Carcinoma | X | 1 | Squamous Cell Papilloma | X | 2 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + A + + + + + + + + | 58 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Tooth | | 1 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 39 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 6| 7| 7| 6| 2| 7| 7| 7| 6| 7| 6| 4| 7| 6| 7| 2| 7| 2| 7| 4| | | DAY ON TEST | 5| 3| 3| 8| 8| 3| 3| 3| 2| 3| 4| 5| 3| 0| 3| 7| 3| 8| 3| 5| | | | 3| 4| 4| 6| 2| 4| 4| 4| 5| 4| 9| 7| 4| 4| 4| 2| 4| 2| 4| 7| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | A | 20 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ CARDIOVASCULAR SYSTEM - cont | | | | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + | 50 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X | 2 | Pericardium, Leiomyosarcoma, | | | Metastatic, Uterus | X | 1 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + A + + + + + + | 48 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + A + + + + + + | 48 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + A + + + A + + | 47 | Pheochromocytoma Malignant | X | 1 | Pheochromocytoma Benign | | 1 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + A + + + A + + | 46 | __________________________________________________________________________|____________| Parathyroid Gland | + + M M + + M + M + + + M + + + | 33 | Carcinoma | | 1 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + I + + + M + + + + | 48 | Pars Distalis, Adenoma | | 5 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + | 49 | Carcinoma, Metastatic, Parathyroid | | | Gland | | 1 | Follicular Cell, Adenoma | | 1 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Ovary | + + + + + + + + + A + + + + A + + + | 58 | Adenoma | X | 1 | Granulosa Cell Tumor Benign | | 1 | Histiocytic Sarcoma | | 2 | Lymphoma Malignant Lymphocytic | | 3 | __________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + | 60 | Histiocytic Sarcoma | X | 2 | Leiomyosarcoma | X | 1 | Lymphoma Malignant Lymphocytic | X | 2 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 40 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 6| 7| 7| 6| 2| 7| 7| 7| 6| 7| 6| 4| 7| 6| 7| 2| 7| 2| 7| 4| | | DAY ON TEST | 5| 3| 3| 8| 8| 3| 3| 3| 2| 3| 4| 5| 3| 0| 3| 7| 3| 8| 3| 5| | | | 3| 4| 4| 6| 2| 4| 4| 4| 5| 4| 9| 7| 4| 4| 4| 2| 4| 2| 4| 7| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | A | 20 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | | | | __________________________________________________________________________|____________| Blood | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + A + + + + + + | 48 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | | 3 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + | 50 | Axillary, Lymphoma Malignant | | | Lymphocytic | | 1 | Iliac, Histiocytic Sarcoma | | 1 | Iliac, Lymphoma Malignant Lymphocytic| | 1 | Renal, Histiocytic Sarcoma | | 2 | Renal, Lymphoma Malignant Lymphocytic| X | 1 | Renal, Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________|____________| Lymph Node, Bronchial | + + + M + + + + + + + M + M + + | 42 | Histiocytic Sarcoma | | 3 | Lymphoma Malignant Lymphocytic | X | 3 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + M + + + | 46 | Histiocytic Sarcoma | | 3 | Lymphoma Malignant Lymphocytic | | 2 | Sarcoma, Metastatic, Skin | | 1 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | M M + M + + M + M + + + + + M M | 33 | Hepatocellular Carcinoma, Metastatic,| | | Liver | | 1 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | | 3 | Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + A + + + + + + | 48 | Histiocytic Sarcoma | X | 5 | Lymphoma Malignant Lymphocytic | X | 4 | Lymphoma Malignant Mixed | X | 3 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + A + + + + + + | 48 | Histiocytic Sarcoma | | 4 | Lymphoma Malignant Lymphocytic | X X | 5 | Lymphoma Malignant Mixed | X | 3 | Lymphoma Malignant Undifferentiated | | | Cell Type | X | 1 | __________________________________________________________________________|____________| Thymus | + + + + + + + + + A + I + + + + | 45 | Lymphoma Malignant Lymphocytic | X X | 6 | Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 41 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 6| 7| 7| 6| 2| 7| 7| 7| 6| 7| 6| 4| 7| 6| 7| 2| 7| 2| 7| 4| | | DAY ON TEST | 5| 3| 3| 8| 8| 3| 3| 3| 2| 3| 4| 5| 3| 0| 3| 7| 3| 8| 3| 5| | | | 3| 4| 4| 6| 2| 4| 4| 4| 5| 4| 9| 7| 4| 4| 4| 2| 4| 2| 4| 7| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | A | 20 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + A + + + + + + | 49 | Adenoacanthoma | X | 2 | Adenocarcinoma | | 2 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + | 50 | Subcutaneous Tissue, Hemangioma | X | 1 | Subcutaneous Tissue, Hemangiosarcoma | | 2 | Subcutaneous Tissue, | | | Neurofibrosarcoma | | 1 | Subcutaneous Tissue, Sarcoma | X | 2 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Skeletal Muscle | | 1 | Adenocarcinoma, Metastatic, Lung | | 1 | Hepatocellular Carcinoma, Metastatic,| | | Liver | | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + | 50 | Histiocytic Sarcoma | | 2 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + | 60 | Adenocarcinoma | | 2 | Adenocarcinoma, Metastatic, | | | Harderian Gland | | 1 | Alveolar/Bronchiolar Adenoma | X X | 9 | Alveolar/Bronchiolar Adenoma, | | | Multiple | X | 3 | Alveolar/Bronchiolar Carcinoma | X X X X X | 10 | Carcinoma, Metastatic, Parathyroid | | | Gland | | 1 | Hepatocellular Carcinoma, Metastatic,| | | Liver | X | 5 | Histiocytic Sarcoma | X | 5 | Lymphoma Malignant Lymphocytic | | 3 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 42 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 6| 7| 7| 6| 2| 7| 7| 7| 6| 7| 6| 4| 7| 6| 7| 2| 7| 2| 7| 4| | | DAY ON TEST | 5| 3| 3| 8| 8| 3| 3| 3| 2| 3| 4| 5| 3| 0| 3| 7| 3| 8| 3| 5| | | | 3| 4| 4| 6| 2| 4| 4| 4| 5| 4| 9| 7| 4| 4| 4| 2| 4| 2| 4| 7| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | A | 20 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - cont | | | | | | Pheochromocytoma Malignant, | | | Metastatic, Adrenal Gland | X | 1 | Fat, Mediastinum, Hemangioma | X | 1 | Mediastinum, Adenocarcinoma, | | | Metastatic, Lung | | 2 | Mediastinum, Hemangiosarcoma, | | | Metastatic, Skin | | 1 | Mediastinum, Hepatocellular | | | Carcinoma, Metastatic, Liver | | 1 | Mediastinum, Leiomyosarcoma, | | | Metastatic, Uterus | X | 1 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + | 48 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Ear | | | __________________________________________________________________________|____________| Eye | | 2 | __________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + M + + | 49 | Adenocarcinoma | | 1 | Adenoma | X | 7 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + A + + + + + + | 48 | Adenocarcinoma, Metastatic, Lung | | 1 | Alveolar/Bronchiolar Carcinoma, | | | Metastatic, Lung | | 1 | Carcinoma, Metastatic, Parathyroid | | | Gland | | 1 | Histiocytic Sarcoma | X | 5 | Lymphoma Malignant Lymphocytic | | 3 | Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + A + + + + + + | 48 | Lymphoma Malignant Lymphocytic | | 3 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + | 60 | Histiocytic Sarcoma | X | 7 | Lymphoma Malignant Lymphocytic | X X | 6 | Lymphoma Malignant Mixed | X X | 4 | Lymphoma Malignant Undifferentiated | | | Cell Type | X | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 43 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 2| 4| 4| 5| 5| 2| 2| 4| 7| 6| 4| 4| 2| 4| 5| 4| 6| 6| 2| 5| 6| 6| 5| 7| DAY ON TEST | 3| 8| 5| 5| 7| 4| 8| 8| 5| 3| 5| 5| 5| 8| 8| 3| 5| 4| 6| 8| 8| 5| 6| 3| 3| | 3| 2| 7| 7| 0| 4| 2| 2| 7| 3| 3| 7| 7| 2| 5| 2| 7| 9| 4| 2| 6| 0| 5| 9| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 62.5 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | M + + + + + M + + + + M + +| __________________________________________________________________________| Gallbladder | + + + + + + + A + + + + M +| __________________________________________________________________________| Intestine Large | + + + + + + + A + + + + A +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + A + + + + A +| Lymphoma Malignant Mixed | X | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + A + + + + A +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + A + + + + A +| __________________________________________________________________________| Intestine Small | + + + + + + + + A + + + + A +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + A + + + + A +| Carcinoma, Multiple | X | Leiomyosarcoma | | Lymphoma Malignant Mixed | | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + A + + + + A +| __________________________________________________________________________| Intestine Small, Jejunum | + A + + + + + + A + + + + A +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | | Hemangiosarcoma, Metastatic, Skin | X | Hepatocellular Carcinoma | | Hepatocellular Adenoma | X | Hepatocellular Adenoma, Multiple | X X | Histiocytic Sarcoma | X | Leiomyosarcoma, Metastatic, | | Intestine Small | | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant | X | Lymphoma Malignant Undifferentiated | | Cell Type | X | __________________________________________________________________________| Mesentery | + +| Histiocytic Sarcoma | | Fat, Leiomyosarcoma, Metastatic, | | Intestine Small | | Fat, Sarcoma, Metastatic, Skin | | __________________________________________________________________________| Pancreas | + + + + + + + A + + + + A +| Acinus, Carcinoma, Metastatic, Ovary | | Acinus, Hemangioma | | Acinus, Histiocytic Sarcoma | | Acinus, Leiomyosarcoma, Metastatic, | | Intestine Small | | Acinus, Sarcoma, Metastatic, Skin | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + A +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + A + + + + + A +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + A + + + + + A +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| Page 44 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 2| 4| 4| 5| 5| 2| 2| 4| 7| 6| 4| 4| 2| 4| 5| 4| 6| 6| 2| 5| 6| 6| 5| 7| DAY ON TEST | 3| 8| 5| 5| 7| 4| 8| 8| 5| 3| 5| 5| 5| 8| 8| 3| 5| 4| 6| 8| 8| 5| 6| 3| 3| | 3| 2| 7| 7| 0| 4| 2| 2| 7| 3| 3| 7| 7| 2| 5| 2| 7| 9| 4| 2| 6| 0| 5| 9| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 62.5 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM - cont | | | | Squamous Cell Carcinoma | | Squamous Cell Papilloma | X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + A + + + + + A +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Tongue | | Squamous Cell Carcinoma | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Blood Vessel | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + A +| Hemangiosarcoma | X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + A +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + A +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + A + + + + A +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + A + + + + A +| Adenoma | X | __________________________________________________________________________| Parathyroid Gland | + + + + + + + M + M + M A M| __________________________________________________________________________| Pituitary Gland | + + + + + + + I + + + + M +| Lymphoma Malignant Lymphocytic | | Pars Distalis, Adenoma | X X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + A +| Follicular Cell, Adenoma | X| _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | __________________________________________________________________________| Tissue NOS | + | Lymphoma Malignant | X | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X X| Carcinoma | | Granulosa Cell Tumor Malignant | | Granulosa Cell Tumor Benign | X X | Hemangioma | | Histiocytic Sarcoma | | Leiomyosarcoma, Metastatic, | | Intestine Small | | Luteoma | | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| Page 45 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 2| 4| 4| 5| 5| 2| 2| 4| 7| 6| 4| 4| 2| 4| 5| 4| 6| 6| 2| 5| 6| 6| 5| 7| DAY ON TEST | 3| 8| 5| 5| 7| 4| 8| 8| 5| 3| 5| 5| 5| 8| 8| 3| 5| 4| 6| 8| 8| 5| 6| 3| 3| | 3| 2| 7| 7| 0| 4| 2| 2| 7| 3| 3| 7| 7| 2| 5| 2| 7| 9| 4| 2| 6| 0| 5| 9| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 62.5 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| GENITAL SYSTEM - cont | | | | Lymphoma Malignant | X | Mixed Tumor Benign | X X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + A +| Adenocarcinoma | | Histiocytic Sarcoma | | Leiomyoma | X | Polyp Stromal | X X | Sarcoma Stromal | | Cervix, Hemangiosarcoma | | __________________________________________________________________________| Vagina | | Hemangiosarcoma | | Leiomyosarcoma | | Squamous Cell Carcinoma | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + A +| Hemangiosarcoma, Metastatic, Skin | X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + +| Renal, Lymphoma Malignant Lymphocytic| | __________________________________________________________________________| Lymph Node, Bronchial | + M + + M M + M + M + + M + + + M M + +| Carcinoma, Metastatic, Ovary | | Hemangiosarcoma, Metastatic, Heart | X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant | X | Sarcoma, Metastatic, Skin | | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + M + + + M + A +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Lymph Node, Mediastinal | M + + + + + M + + + + + + M M M + + A M| Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | X | Carcinoma, Metastatic, Ovary | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + A + + M + + +| Carcinoma, Metastatic, Ovary | | Histiocytic Sarcoma | X | Leiomyosarcoma, Metastatic, | | Intestine Small | | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant | X | _____________________________________________________________________________________________________________________| Page 46 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 2| 4| 4| 5| 5| 2| 2| 4| 7| 6| 4| 4| 2| 4| 5| 4| 6| 6| 2| 5| 6| 6| 5| 7| DAY ON TEST | 3| 8| 5| 5| 7| 4| 8| 8| 5| 3| 5| 5| 5| 8| 8| 3| 5| 4| 6| 8| 8| 5| 6| 3| 3| | 3| 2| 7| 7| 0| 4| 2| 2| 7| 3| 3| 7| 7| 2| 5| 2| 7| 9| 4| 2| 6| 0| 5| 9| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 62.5 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Lymphoma Malignant Mixed | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + A + + + + A +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | | Lymphoma Malignant Undifferentiated | | Cell Type | X | __________________________________________________________________________| Thymus | + + + + + + M + + + + + + + + + + M A +| Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + +| Adenoacanthoma | | Adenocarcinoma | X | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Hemangioma | | Subcutaneous Tissue, Hemangiosarcoma | | Subcutaneous Tissue, Hemangiosarcoma,| | Multiple | X | Subcutaneous Tissue, Sarcoma | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Skin | X | __________________________________________________________________________| Skeletal Muscle | | Diaphragm, Leiomyosarcoma, | | Metastatic, Intestine Small | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant | X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + A +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X | Adenocarcinoma, Metastatic, Mammary | | Gland | X | Alveolar/Bronchiolar Adenoma | X X X X X X X | Alveolar/Bronchiolar Adenoma, | | Multiple | X | _____________________________________________________________________________________________________________________| Page 47 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 2| 4| 4| 5| 5| 2| 2| 4| 7| 6| 4| 4| 2| 4| 5| 4| 6| 6| 2| 5| 6| 6| 5| 7| DAY ON TEST | 3| 8| 5| 5| 7| 4| 8| 8| 5| 3| 5| 5| 5| 8| 8| 3| 5| 4| 6| 8| 8| 5| 6| 3| 3| | 3| 2| 7| 7| 0| 4| 2| 2| 7| 3| 3| 7| 7| 2| 5| 2| 7| 9| 4| 2| 6| 0| 5| 9| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 62.5 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Alveolar/Bronchiolar Carcinoma | X X | Alveolar/Bronchiolar Carcinoma, | | Multiple | | Carcinoma, Metastatic, Ovary | | Granulosa Cell Tumor Malignant, | | Metastatic, Ovary | | Hepatocellular Carcinoma, Metastatic,| | Liver | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant | X | Sarcoma, Metastatic, Skin | | Mediastinum, Adenocarcinoma, | | Metastatic, Lung | | Mediastinum, Alveolar/Bronchiolar | | Carcinoma, Metastatic, Lung | X | Mediastinum, Hemangiosarcoma, | | Metastatic, Heart | X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | Lymphoma Malignant | X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + A +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + +| Adenoma | X X X X X X X | Bilateral, Adenoma | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant | X | __________________________________________________________________________| Urinary Bladder | + + + + + + + A + + + + A +| Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant | X | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | X | Lymphoma Malignant Undifferentiated | | Cell Type | X | _____________________________________________________________________________________________________________________| Page 48 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 6| 6| 6| 6| 6| 6| 6| 7| 2| 7| 5| 4| 4| 7| 4| 4| 2| 4| 2| 3| 6| 4| 6| 6| DAY ON TEST | 2| 7| 4| 9| 1| 3| 8| 2| 3| 8| 3| 4| 1| 5| 3| 8| 5| 8| 0| 8| 9| 0| 4| 5| 2| | 2| 0| 5| 8| 0| 9| 0| 5| 3| 2| 3| 6| 8| 7| 3| 5| 7| 2| 7| 2| 2| 8| 3| 8| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 62.5 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + M A + + + + + I + + + + + + + +| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Mixed | | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + A + + + + + + + + + + + + + +| Carcinoma, Multiple | | Leiomyosarcoma | X | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | | Hemangiosarcoma, Metastatic, Skin | | Hepatocellular Carcinoma | X X X X X X| Hepatocellular Adenoma | X X X X | Hepatocellular Adenoma, Multiple | X X | Histiocytic Sarcoma | | Leiomyosarcoma, Metastatic, | | Intestine Small | X | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant | | Lymphoma Malignant Undifferentiated | | Cell Type | | __________________________________________________________________________| Mesentery | + + + + + | Histiocytic Sarcoma | X | Fat, Leiomyosarcoma, Metastatic, | | Intestine Small | X | Fat, Sarcoma, Metastatic, Skin | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + +| Acinus, Carcinoma, Metastatic, Ovary | | Acinus, Hemangioma | | Acinus, Histiocytic Sarcoma | X | Acinus, Leiomyosarcoma, Metastatic, | | Intestine Small | X | Acinus, Sarcoma, Metastatic, Skin | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| Page 49 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 6| 6| 6| 6| 6| 6| 6| 7| 2| 7| 5| 4| 4| 7| 4| 4| 2| 4| 2| 3| 6| 4| 6| 6| DAY ON TEST | 2| 7| 4| 9| 1| 3| 8| 2| 3| 8| 3| 4| 1| 5| 3| 8| 5| 8| 0| 8| 9| 0| 4| 5| 2| | 2| 0| 5| 8| 0| 9| 0| 5| 3| 2| 3| 6| 8| 7| 3| 5| 7| 2| 7| 2| 2| 8| 3| 8| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 62.5 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM - cont | | | | Squamous Cell Carcinoma | | Squamous Cell Papilloma | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Tongue | | Squamous Cell Carcinoma | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Blood Vessel | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Adrenal Gland, Medulla | I + + I + + + + + + + + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + I + + + + + + + + + + + + +| Adenoma | | __________________________________________________________________________| Parathyroid Gland | M + + + + M + + + + + + + + + M + + + M| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | Pars Distalis, Adenoma | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + +| Follicular Cell, Adenoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | __________________________________________________________________________| Tissue NOS | | Lymphoma Malignant | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | Carcinoma | | Granulosa Cell Tumor Malignant | X X X | Granulosa Cell Tumor Benign | X X X | Hemangioma | X | Histiocytic Sarcoma | | Leiomyosarcoma, Metastatic, | | Intestine Small | X | Luteoma | | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| Page 50 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 6| 6| 6| 6| 6| 6| 6| 7| 2| 7| 5| 4| 4| 7| 4| 4| 2| 4| 2| 3| 6| 4| 6| 6| DAY ON TEST | 2| 7| 4| 9| 1| 3| 8| 2| 3| 8| 3| 4| 1| 5| 3| 8| 5| 8| 0| 8| 9| 0| 4| 5| 2| | 2| 0| 5| 8| 0| 9| 0| 5| 3| 2| 3| 6| 8| 7| 3| 5| 7| 2| 7| 2| 2| 8| 3| 8| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 62.5 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| GENITAL SYSTEM - cont | | | | Lymphoma Malignant | | Mixed Tumor Benign | | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | | Histiocytic Sarcoma | X | Leiomyoma | | Polyp Stromal | | Sarcoma Stromal | X | Cervix, Hemangiosarcoma | | __________________________________________________________________________| Vagina | + | Hemangiosarcoma | | Leiomyosarcoma | X | Squamous Cell Carcinoma | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Skin | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + +| Renal, Lymphoma Malignant Lymphocytic| X | __________________________________________________________________________| Lymph Node, Bronchial | + + M M + + + M + + + M + M + M + + + + + M| Carcinoma, Metastatic, Ovary | | Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant | | Sarcoma, Metastatic, Skin | | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + M + + + M + + + + + I| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Lymph Node, Mediastinal | + + M + + + M M + + + + M + + M + + M + + +| Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | | Carcinoma, Metastatic, Ovary | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Ovary | | Histiocytic Sarcoma | | Leiomyosarcoma, Metastatic, | | Intestine Small | X | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant | | _____________________________________________________________________________________________________________________| Page 51 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 6| 6| 6| 6| 6| 6| 6| 7| 2| 7| 5| 4| 4| 7| 4| 4| 2| 4| 2| 3| 6| 4| 6| 6| DAY ON TEST | 2| 7| 4| 9| 1| 3| 8| 2| 3| 8| 3| 4| 1| 5| 3| 8| 5| 8| 0| 8| 9| 0| 4| 5| 2| | 2| 0| 5| 8| 0| 9| 0| 5| 3| 2| 3| 6| 8| 7| 3| 5| 7| 2| 7| 2| 2| 8| 3| 8| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 62.5 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | X | Lymphoma Malignant Undifferentiated | | Cell Type | | __________________________________________________________________________| Thymus | M + + + + + + + + + + + + + + + + + + + M M| Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + +| Adenoacanthoma | X X X | Adenocarcinoma | X X X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Hemangioma | | Subcutaneous Tissue, Hemangiosarcoma | | Subcutaneous Tissue, Hemangiosarcoma,| | Multiple | | Subcutaneous Tissue, Sarcoma | X X| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Skin | | __________________________________________________________________________| Skeletal Muscle | + | Diaphragm, Leiomyosarcoma, | | Metastatic, Intestine Small | X | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + I + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X| Adenocarcinoma, Metastatic, Mammary | | Gland | | Alveolar/Bronchiolar Adenoma | X X X X X | Alveolar/Bronchiolar Adenoma, | | Multiple | X | _____________________________________________________________________________________________________________________| Page 52 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 6| 6| 6| 6| 6| 6| 6| 7| 2| 7| 5| 4| 4| 7| 4| 4| 2| 4| 2| 3| 6| 4| 6| 6| DAY ON TEST | 2| 7| 4| 9| 1| 3| 8| 2| 3| 8| 3| 4| 1| 5| 3| 8| 5| 8| 0| 8| 9| 0| 4| 5| 2| | 2| 0| 5| 8| 0| 9| 0| 5| 3| 2| 3| 6| 8| 7| 3| 5| 7| 2| 7| 2| 2| 8| 3| 8| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 62.5 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Alveolar/Bronchiolar Carcinoma | X X | Alveolar/Bronchiolar Carcinoma, | | Multiple | X X | Carcinoma, Metastatic, Ovary | | Granulosa Cell Tumor Malignant, | | Metastatic, Ovary | X | Hepatocellular Carcinoma, Metastatic,| | Liver | X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant | | Sarcoma, Metastatic, Skin | X | Mediastinum, Adenocarcinoma, | | Metastatic, Lung | X| Mediastinum, Alveolar/Bronchiolar | | Carcinoma, Metastatic, Lung | | Mediastinum, Hemangiosarcoma, | | Metastatic, Heart | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | Lymphoma Malignant | | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X X X X | Bilateral, Adenoma | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant | | __________________________________________________________________________| Urinary Bladder | + + + + + I + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant | | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | X | Lymphoma Malignant Undifferentiated | | Cell Type | | _____________________________________________________________________________________________________________________| Page 53 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 5| 7| 5| 5| 2| 5| 4| 7| 2| 5| 6| 3| 7| 6| 4| 4| 7| 7| 7| | | DAY ON TEST | 2| 6| 3| 9| 7| 8| 9| 4| 3| 8| 2| 5| 8| 0| 9| 5| 5| 3| 3| 3| | | | 3| 9| 3| 4| 9| 2| 7| 3| 3| 2| 4| 3| 7| 7| 8| 7| 7| 3| 3| 3| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 62.5 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | M + + + + + + + + + M + + + + + | 45 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + + A + + M + + + + | 43 | __________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + + A + + + + | 47 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + A + + + + | 47 | Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + A + + + + | 47 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + A + + + + | 47 | __________________________________________________________________________|____________| Intestine Small | + + + + + + + + + + + A + + + + | 48 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + A + + + + | 46 | Carcinoma, Multiple | | 1 | Leiomyosarcoma | | 1 | Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + A + + + + | 47 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + A + + + + | 47 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + | 61 | Hemangiosarcoma | X | 1 | Hemangiosarcoma, Metastatic, Skin | | 1 | Hepatocellular Carcinoma | X X X | 9 | Hepatocellular Adenoma | X X X X X | 10 | Hepatocellular Adenoma, Multiple | X | 5 | Histiocytic Sarcoma | X X | 3 | Leiomyosarcoma, Metastatic, | | | Intestine Small | | 1 | Lymphoma Malignant Lymphocytic | X | 3 | Lymphoma Malignant | | 1 | Lymphoma Malignant Undifferentiated | | | Cell Type | | 1 | __________________________________________________________________________|____________| Mesentery | + + | 9 | Histiocytic Sarcoma | | 1 | Fat, Leiomyosarcoma, Metastatic, | | | Intestine Small | | 1 | Fat, Sarcoma, Metastatic, Skin | X | 1 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + | 48 | Acinus, Carcinoma, Metastatic, Ovary | X | 1 | Acinus, Hemangioma | X | 1 | Acinus, Histiocytic Sarcoma | X | 2 | Acinus, Leiomyosarcoma, Metastatic, | | | Intestine Small | | 1 | Acinus, Sarcoma, Metastatic, Skin | X | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 54 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 5| 7| 5| 5| 2| 5| 4| 7| 2| 5| 6| 3| 7| 6| 4| 4| 7| 7| 7| | | DAY ON TEST | 2| 6| 3| 9| 7| 8| 9| 4| 3| 8| 2| 5| 8| 0| 9| 5| 5| 3| 3| 3| | | | 3| 9| 3| 4| 9| 2| 7| 3| 3| 2| 4| 3| 7| 7| 8| 7| 7| 3| 3| 3| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 62.5 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + | 68 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X | 2 | Squamous Cell Carcinoma | X | 1 | Squamous Cell Papilloma | X | 2 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + | 68 | Lymphoma Malignant Lymphocytic | X | 1 | __________________________________________________________________________|____________| Tongue | + | 1 | Squamous Cell Carcinoma | X | 1 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Blood Vessel | + | 1 | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + | 59 | Hemangiosarcoma | | 1 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant Lymphocytic | X | 2 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + | 49 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + I + + + + + + + + | 45 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + | 47 | Adenoma | | 1 | __________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + M M + + + | 39 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + M + + + + | 47 | Lymphoma Malignant Lymphocytic | | 1 | Pars Distalis, Adenoma | X X | 4 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + | 49 | Follicular Cell, Adenoma | X | 2 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | __________________________________________________________________________|____________| Tissue NOS | | 1 | Lymphoma Malignant | | 1 | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 55 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 5| 7| 5| 5| 2| 5| 4| 7| 2| 5| 6| 3| 7| 6| 4| 4| 7| 7| 7| | | DAY ON TEST | 2| 6| 3| 9| 7| 8| 9| 4| 3| 8| 2| 5| 8| 0| 9| 5| 5| 3| 3| 3| | | | 3| 9| 3| 4| 9| 2| 7| 3| 3| 2| 4| 3| 7| 7| 8| 7| 7| 3| 3| 3| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 62.5 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ GENITAL SYSTEM - cont | | | | | | Adenoma | | 3 | Carcinoma | X | 1 | Granulosa Cell Tumor Malignant | | 3 | Granulosa Cell Tumor Benign | X X | 7 | Hemangioma | X | 2 | Histiocytic Sarcoma | X X | 2 | Leiomyosarcoma, Metastatic, | | | Intestine Small | | 1 | Luteoma | X | 1 | Lymphoma Malignant Lymphocytic | | 1 | Lymphoma Malignant | | 1 | Mixed Tumor Benign | | 2 | __________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + | 69 | Adenocarcinoma | X X | 2 | Histiocytic Sarcoma | | 1 | Leiomyoma | | 1 | Polyp Stromal | X | 3 | Sarcoma Stromal | | 1 | Cervix, Hemangiosarcoma | X | 1 | __________________________________________________________________________|____________| Vagina | + + | 3 | Hemangiosarcoma | X | 1 | Leiomyosarcoma | | 1 | Squamous Cell Carcinoma | X | 1 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Blood | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + | 59 | Hemangiosarcoma, Metastatic, Skin | | 1 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + + + | 60 | Renal, Lymphoma Malignant Lymphocytic| | 1 | __________________________________________________________________________|____________| Lymph Node, Bronchial | + + + M + + + + + + + M + M M + + + | 41 | Carcinoma, Metastatic, Ovary | X | 1 | Hemangiosarcoma, Metastatic, Heart | | 1 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant Lymphocytic | | 1 | Lymphoma Malignant | | 1 | Sarcoma, Metastatic, Skin | X | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 56 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 5| 7| 5| 5| 2| 5| 4| 7| 2| 5| 6| 3| 7| 6| 4| 4| 7| 7| 7| | | DAY ON TEST | 2| 6| 3| 9| 7| 8| 9| 4| 3| 8| 2| 5| 8| 0| 9| 5| 5| 3| 3| 3| | | | 3| 9| 3| 4| 9| 2| 7| 3| 3| 2| 4| 3| 7| 7| 8| 7| 7| 3| 3| 3| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 62.5 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + M + + M + M + M M + + + | 49 | Lymphoma Malignant Lymphocytic | X | 3 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | + + + + + + + + + M + + + + + M + M | 44 | Alveolar/Bronchiolar Carcinoma, | | | Metastatic, Lung | | 1 | Carcinoma, Metastatic, Ovary | X | 1 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant Lymphocytic | X | 2 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + | 58 | Carcinoma, Metastatic, Ovary | X | 1 | Histiocytic Sarcoma | X X | 3 | Leiomyosarcoma, Metastatic, | | | Intestine Small | | 1 | Lymphoma Malignant Lymphocytic | | 2 | Lymphoma Malignant | | 1 | Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + | 58 | Histiocytic Sarcoma | X X | 3 | Lymphoma Malignant Lymphocytic | | 2 | Lymphoma Malignant Mixed | | 1 | Lymphoma Malignant Undifferentiated | | | Cell Type | | 1 | __________________________________________________________________________|____________| Thymus | M + + + + + + + + + I I + + + + + + | 51 | Lymphoma Malignant Lymphocytic | X | 2 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + + + + | 61 | Adenoacanthoma | X X X | 6 | Adenocarcinoma | X X | 6 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + | 53 | Subcutaneous Tissue, Hemangioma | X | 1 | Subcutaneous Tissue, Hemangiosarcoma | X | 1 | Subcutaneous Tissue, Hemangiosarcoma,| | | Multiple | | 1 | Subcutaneous Tissue, Sarcoma | X X | 5 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 57 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 5| 7| 5| 5| 2| 5| 4| 7| 2| 5| 6| 3| 7| 6| 4| 4| 7| 7| 7| | | DAY ON TEST | 2| 6| 3| 9| 7| 8| 9| 4| 3| 8| 2| 5| 8| 0| 9| 5| 5| 3| 3| 3| | | | 3| 9| 3| 4| 9| 2| 7| 3| 3| 2| 4| 3| 7| 7| 8| 7| 7| 3| 3| 3| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 62.5 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ MUSCULOSKELETAL SYSTEM - cont | | | | | | Hemangiosarcoma, Metastatic, Skin | | 1 | __________________________________________________________________________|____________| Skeletal Muscle | | 1 | Diaphragm, Leiomyosarcoma, | | | Metastatic, Intestine Small | | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + | 50 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X | 2 | Lymphoma Malignant | | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + + + + + + I + + | 47 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + | 70 | Adenocarcinoma | | 2 | Adenocarcinoma, Metastatic, Mammary | | | Gland | | 1 | Alveolar/Bronchiolar Adenoma | X X X X | 16 | Alveolar/Bronchiolar Adenoma, | | | Multiple | X X | 4 | Alveolar/Bronchiolar Carcinoma | X | 5 | Alveolar/Bronchiolar Carcinoma, | | | Multiple | | 2 | Carcinoma, Metastatic, Ovary | X | 1 | Granulosa Cell Tumor Malignant, | | | Metastatic, Ovary | | 1 | Hepatocellular Carcinoma, Metastatic,| | | Liver | X | 2 | Histiocytic Sarcoma | X X | 3 | Lymphoma Malignant Lymphocytic | X | 3 | Lymphoma Malignant | | 1 | Sarcoma, Metastatic, Skin | | 1 | Mediastinum, Adenocarcinoma, | | | Metastatic, Lung | | 1 | Mediastinum, Alveolar/Bronchiolar | | | Carcinoma, Metastatic, Lung | | 1 | Mediastinum, Hemangiosarcoma, | | | Metastatic, Heart | | 1 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 58 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 5| 7| 5| 5| 2| 5| 4| 7| 2| 5| 6| 3| 7| 6| 4| 4| 7| 7| 7| | | DAY ON TEST | 2| 6| 3| 9| 7| 8| 9| 4| 3| 8| 2| 5| 8| 0| 9| 5| 5| 3| 3| 3| | | | 3| 9| 3| 4| 9| 2| 7| 3| 3| 2| 4| 3| 7| 7| 8| 7| 7| 3| 3| 3| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 62.5 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - cont | | | | | | Lymphoma Malignant Lymphocytic | X | 1 | Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + | 49 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | | 2 | __________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + | 59 | Adenoma | X X | 14 | Bilateral, Adenoma | X | 2 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + | 50 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | | 1 | Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + A + + + + | 46 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + | 70 | Histiocytic Sarcoma | X X | 4 | Lymphoma Malignant | | 1 | Lymphoma Malignant Lymphocytic | X | 3 | Lymphoma Malignant Mixed | | 2 | Lymphoma Malignant Undifferentiated | | | Cell Type | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 59 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 5| 2| 5| 4| 4| 4| 3| 4| 4| 4| 4| 2| 4| 6| 4| 5| 3| 5| 2| 4| 5| 5| 4| 2| 4| DAY ON TEST | 6| 8| 0| 5| 5| 6| 9| 7| 5| 8| 5| 8| 5| 2| 6| 1| 7| 9| 8| 5| 9| 6| 1| 8| 5| | 9| 2| 2| 7| 7| 0| 3| 1| 7| 5| 7| 2| 4| 9| 3| 3| 1| 7| 2| 7| 7| 9| 1| 2| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 200 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + A + + + + + + + A + + + + + + + +| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Leukemia Granulocytic | | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + +| Leiomyosarcoma, Metastatic, Stomach | X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Intestine Small, Jejunum | + + + + A + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Leiomyosarcoma, Metastatic, Stomach | X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma, Multiple | | Hemangiosarcoma, Metastatic, Heart | X X X | Hepatocellular Carcinoma | X X | Hepatocellular Carcinoma, Multiple | X | Hepatocellular Adenoma | X X| Hepatocellular Adenoma, Multiple | X X | Histiocytic Sarcoma | X | Leiomyosarcoma, Metastatic, Stomach | X | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Mesentery | + + + + | Fat, Hemangiosarcoma, Metastatic, | | Heart | X | Fat, Histiocytic Sarcoma | | Fat, Leiomyosarcoma, Metastatic, | | Stomach | X | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + +| Acinus, Leiomyosarcoma, Metastatic, | | Stomach | X | Acinus, Leukemia Granulocytic | | Acinus, Lymphoma Malignant | | Lymphocytic | X X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + +| Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 60 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 5| 2| 5| 4| 4| 4| 3| 4| 4| 4| 4| 2| 4| 6| 4| 5| 3| 5| 2| 4| 5| 5| 4| 2| 4| DAY ON TEST | 6| 8| 0| 5| 5| 6| 9| 7| 5| 8| 5| 8| 5| 2| 6| 1| 7| 9| 8| 5| 9| 6| 1| 8| 5| | 9| 2| 2| 7| 7| 0| 3| 1| 7| 5| 7| 2| 4| 9| 3| 3| 1| 7| 2| 7| 7| 9| 1| 2| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 200 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM - cont | | | | Leiomyosarcoma | X | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X | Squamous Cell Carcinoma | | Squamous Cell Papilloma | X X | Squamous Cell Papilloma, Multiple | | __________________________________________________________________________| Stomach, Glandular | + + + + + + M + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X X X X X X X | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Histiocytic Sarcoma | X | Leiomyosarcoma, Metastatic, Stomach | X | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X | Sarcoma, Metastatic, Skin | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Leiomyosarcoma, Metastatic, Stomach | X | Leukemia Granulocytic | | Pheochromocytoma Benign | X | Sarcoma, Metastatic, Skin | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + +| Adenoma | | __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + + M + + + + + M + + M| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + I + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + +| Follicular Cell, Adenoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | _____________________________________________________________________________________________________________________| Page 61 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 5| 2| 5| 4| 4| 4| 3| 4| 4| 4| 4| 2| 4| 6| 4| 5| 3| 5| 2| 4| 5| 5| 4| 2| 4| DAY ON TEST | 6| 8| 0| 5| 5| 6| 9| 7| 5| 8| 5| 8| 5| 2| 6| 1| 7| 9| 8| 5| 9| 6| 1| 8| 5| | 9| 2| 2| 7| 7| 0| 3| 1| 7| 5| 7| 2| 4| 9| 3| 3| 1| 7| 2| 7| 7| 9| 1| 2| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 200 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| GENITAL SYSTEM - cont | | | | Granulosa Cell Tumor Malignant | X | Granulosa Cell Tumor Benign | X X X | Histiocytic Sarcoma | X | Leiomyosarcoma, Metastatic, Stomach | X | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X X | Mixed Tumor Benign | X X X X | Bilateral, Granulosa Cell Tumor | | Benign | X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | | Hemangioma | X | Histiocytic Sarcoma | | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X | Polyp Stromal | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| Axillary, Lymphoma Malignant | | Lymphocytic | X X | Axillary, Sarcoma, Metastatic, Skin | | Inguinal, Lymphoma Malignant | | Lymphocytic | X X | Pancreatic, Histiocytic Sarcoma | | Pancreatic, Leiomyosarcoma, | | Metastatic, Stomach | X | __________________________________________________________________________| Lymph Node, Bronchial | + M + + M M M M + + + + + + M + + + + M + + + + M| Adenocarcinoma, Metastatic, Lung | | Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Lymph Node, Mandibular | M + + M + + + M M + + + + + + + I + + M + + + + +| Histiocytic Sarcoma | | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Lymph Node, Mediastinal | + + I + M + + + + + + M + + + + + + M + + M + + +| Alveolar/Bronchiolar Carcinoma, | | _____________________________________________________________________________________________________________________| Page 62 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 5| 2| 5| 4| 4| 4| 3| 4| 4| 4| 4| 2| 4| 6| 4| 5| 3| 5| 2| 4| 5| 5| 4| 2| 4| DAY ON TEST | 6| 8| 0| 5| 5| 6| 9| 7| 5| 8| 5| 8| 5| 2| 6| 1| 7| 9| 8| 5| 9| 6| 1| 8| 5| | 9| 2| 2| 7| 7| 0| 3| 1| 7| 5| 7| 2| 4| 9| 3| 3| 1| 7| 2| 7| 7| 9| 1| 2| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 200 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Metastatic, Lung | | Histiocytic Sarcoma | X | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + M + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Leiomyosarcoma, Metastatic, Stomach | X | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant Mixed | X| __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Thymus | I + + + + + + M + + + + + M + + + + + + + + + + M| Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + +| Adenoacanthoma | X X | Adenocarcinoma | X X X X X X | Adenocarcinoma, Multiple | X| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + +| Leiomyosarcoma, Metastatic, Stomach | X | Sebaceous Gland, Adenoma | | Subcutaneous Tissue, Hemangiosarcoma | X | Subcutaneous Tissue, Sarcoma | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | + | Diaphragm, Sarcoma, Metastatic, Skin | X | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Spinal Cord | + | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | _____________________________________________________________________________________________________________________| Page 63 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 5| 2| 5| 4| 4| 4| 3| 4| 4| 4| 4| 2| 4| 6| 4| 5| 3| 5| 2| 4| 5| 5| 4| 2| 4| DAY ON TEST | 6| 8| 0| 5| 5| 6| 9| 7| 5| 8| 5| 8| 5| 2| 6| 1| 7| 9| 8| 5| 9| 6| 1| 8| 5| | 9| 2| 2| 7| 7| 0| 3| 1| 7| 5| 7| 2| 4| 9| 3| 3| 1| 7| 2| 7| 7| 9| 1| 2| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 200 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Multiple | | Adenocarcinoma, Metastatic, | | Harderian Gland | | Adenocarcinoma, Metastatic, Mammary | | Gland | X | Alveolar/Bronchiolar Adenoma | X| Alveolar/Bronchiolar Adenoma, | | Multiple | X X | Alveolar/Bronchiolar Carcinoma | X X X X| Alveolar/Bronchiolar Carcinoma, | | Multiple | X | Granulosa Cell Tumor Malignant, | | Metastatic, Ovary | X | Hemangiosarcoma, Metastatic, Heart | X X X X | Hepatocellular Carcinoma, Metastatic,| | Liver | X X | Histiocytic Sarcoma | X | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X X | Sarcoma, Metastatic, Skin | | Mediastinum, Alveolar/Bronchiolar | | Carcinoma, Metastatic, Lung | | Mediastinum, Hemangiosarcoma, | | Metastatic, Heart | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | | __________________________________________________________________________| Eye | + | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | | Adenoma | X X X X X X X X X| Bilateral, Adenoma | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | X | Histiocytic Sarcoma | X | Leiomyosarcoma, Metastatic, Stomach | X | Leukemia Granulocytic | | _____________________________________________________________________________________________________________________| Page 64 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 5| 2| 5| 4| 4| 4| 3| 4| 4| 4| 4| 2| 4| 6| 4| 5| 3| 5| 2| 4| 5| 5| 4| 2| 4| DAY ON TEST | 6| 8| 0| 5| 5| 6| 9| 7| 5| 8| 5| 8| 5| 2| 6| 1| 7| 9| 8| 5| 9| 6| 1| 8| 5| | 9| 2| 2| 7| 7| 0| 3| 1| 7| 5| 7| 2| 4| 9| 3| 3| 1| 7| 2| 7| 7| 9| 1| 2| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 200 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| URINARY SYSTEM - cont | | | | Lymphoma Malignant Lymphocytic | X X | Renal Tubule, Adenoma | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant Mixed | X| _____________________________________________________________________________________________________________________| Page 65 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 3| 5| 5| 4| 4| 6| 5| 4| 4| 4| 3| 4| 4| 4| 2| 4| 5| 5| 6| 0| 2| 7| 5| 2| DAY ON TEST | 8| 5| 6| 6| 5| 5| 6| 0| 7| 8| 6| 7| 0| 1| 5| 0| 5| 2| 3| 2| 0| 8| 0| 0| 8| | 2| 3| 9| 9| 7| 7| 3| 2| 2| 5| 4| 3| 8| 8| 7| 5| 7| 4| 2| 4| 9| 2| 4| 9| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 200 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Gallbladder | A + + + + + + + + A + A + + + + + + + A A + | __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + A + + | __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + A A + | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + A + + + + + + + A + + | __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + I + + + A + + | __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + + + A + + | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + M + I + + + + + + + A A + | Histiocytic Sarcoma | | Leukemia Granulocytic | | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + A + I + + + + + + + A + + | Leiomyosarcoma, Metastatic, Stomach | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + A + + | Histiocytic Sarcoma | X | Leiomyosarcoma, Metastatic, Stomach | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + | Hemangiosarcoma, Multiple | | Hemangiosarcoma, Metastatic, Heart | X X X X X | Hepatocellular Carcinoma | X X X X X | Hepatocellular Carcinoma, Multiple | | Hepatocellular Adenoma | X X X X X X | Hepatocellular Adenoma, Multiple | X X | Histiocytic Sarcoma | X X X X | Leiomyosarcoma, Metastatic, Stomach | | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Mesentery | + + + + + + + + + | Fat, Hemangiosarcoma, Metastatic, | | Heart | | Fat, Histiocytic Sarcoma | X X | Fat, Leiomyosarcoma, Metastatic, | | Stomach | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + A + + | Acinus, Leiomyosarcoma, Metastatic, | | Stomach | | Acinus, Leukemia Granulocytic | | Acinus, Lymphoma Malignant | | Lymphocytic | X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 66 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 3| 5| 5| 4| 4| 6| 5| 4| 4| 4| 3| 4| 4| 4| 2| 4| 5| 5| 6| 0| 2| 7| 5| 2| DAY ON TEST | 8| 5| 6| 6| 5| 5| 6| 0| 7| 8| 6| 7| 0| 1| 5| 0| 5| 2| 3| 2| 0| 8| 0| 0| 8| | 2| 3| 9| 9| 7| 7| 3| 2| 2| 5| 4| 3| 8| 8| 7| 5| 7| 4| 2| 4| 9| 2| 4| 9| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 200 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM - cont | | | | Leiomyosarcoma | | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | | Squamous Cell Carcinoma | X | Squamous Cell Papilloma | X | Squamous Cell Papilloma, Multiple | X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + A + + + +| Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X X X X X X X X | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + | Adenoma | X | Histiocytic Sarcoma | X X | Leiomyosarcoma, Metastatic, Stomach | | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | | Sarcoma, Metastatic, Skin | X | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + | Histiocytic Sarcoma | X | Leiomyosarcoma, Metastatic, Stomach | | Leukemia Granulocytic | | Pheochromocytoma Benign | | Sarcoma, Metastatic, Skin | X | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + A + + | Adenoma | X X | __________________________________________________________________________| Parathyroid Gland | + M + + + + M M + + M + + + M M + + + M + + | __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + M + + | Follicular Cell, Adenoma | X X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | _____________________________________________________________________________________________________________________| Page 67 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 3| 5| 5| 4| 4| 6| 5| 4| 4| 4| 3| 4| 4| 4| 2| 4| 5| 5| 6| 0| 2| 7| 5| 2| DAY ON TEST | 8| 5| 6| 6| 5| 5| 6| 0| 7| 8| 6| 7| 0| 1| 5| 0| 5| 2| 3| 2| 0| 8| 0| 0| 8| | 2| 3| 9| 9| 7| 7| 3| 2| 2| 5| 4| 3| 8| 8| 7| 5| 7| 4| 2| 4| 9| 2| 4| 9| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 200 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| GENITAL SYSTEM - cont | | | | Granulosa Cell Tumor Malignant | X | Granulosa Cell Tumor Benign | X X X | Histiocytic Sarcoma | X X | Leiomyosarcoma, Metastatic, Stomach | | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X | Mixed Tumor Benign | X X | Bilateral, Granulosa Cell Tumor | | Benign | | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | | Hemangioma | | Histiocytic Sarcoma | X | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | | Polyp Stromal | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + M + + + +| Axillary, Lymphoma Malignant | | Lymphocytic | | Axillary, Sarcoma, Metastatic, Skin | X | Inguinal, Lymphoma Malignant | | Lymphocytic | | Pancreatic, Histiocytic Sarcoma | | Pancreatic, Leiomyosarcoma, | | Metastatic, Stomach | | __________________________________________________________________________| Lymph Node, Bronchial | + + + + + M M + I + M + + + + + M M + + M + + M M| Adenocarcinoma, Metastatic, Lung | X | Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | | Histiocytic Sarcoma | X X X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + M + M + + + + + I + + + + + M + + + +| Histiocytic Sarcoma | X | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Lymph Node, Mediastinal | + + + + M + M M + + + + + + + + + + + M M M + M M| Alveolar/Bronchiolar Carcinoma, | | _____________________________________________________________________________________________________________________| Page 68 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 3| 5| 5| 4| 4| 6| 5| 4| 4| 4| 3| 4| 4| 4| 2| 4| 5| 5| 6| 0| 2| 7| 5| 2| DAY ON TEST | 8| 5| 6| 6| 5| 5| 6| 0| 7| 8| 6| 7| 0| 1| 5| 0| 5| 2| 3| 2| 0| 8| 0| 0| 8| | 2| 3| 9| 9| 7| 7| 3| 2| 2| 5| 4| 3| 8| 8| 7| 5| 7| 4| 2| 4| 9| 2| 4| 9| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 200 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Metastatic, Lung | | Histiocytic Sarcoma | X X | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + M + + + M + + + + + M + + + +| Histiocytic Sarcoma | X X X X | Leiomyosarcoma, Metastatic, Stomach | | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant Mixed | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X X | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Thymus | + + + + M + + + + M + + + + + + + M + + + + + + +| Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + M + + + + + + + + + + + A + + | Adenoacanthoma | | Adenocarcinoma | X X X X | Adenocarcinoma, Multiple | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + | Leiomyosarcoma, Metastatic, Stomach | | Sebaceous Gland, Adenoma | X | Subcutaneous Tissue, Hemangiosarcoma | X | Subcutaneous Tissue, Sarcoma | X X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Skeletal Muscle | + | Diaphragm, Sarcoma, Metastatic, Skin | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + | Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Spinal Cord | | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | _____________________________________________________________________________________________________________________| Page 69 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 3| 5| 5| 4| 4| 6| 5| 4| 4| 4| 3| 4| 4| 4| 2| 4| 5| 5| 6| 0| 2| 7| 5| 2| DAY ON TEST | 8| 5| 6| 6| 5| 5| 6| 0| 7| 8| 6| 7| 0| 1| 5| 0| 5| 2| 3| 2| 0| 8| 0| 0| 8| | 2| 3| 9| 9| 7| 7| 3| 2| 2| 5| 4| 3| 8| 8| 7| 5| 7| 4| 2| 4| 9| 2| 4| 9| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 200 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + + + + A + | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Multiple | X | Adenocarcinoma, Metastatic, | | Harderian Gland | | Adenocarcinoma, Metastatic, Mammary | | Gland | | Alveolar/Bronchiolar Adenoma | X X X X X X X | Alveolar/Bronchiolar Adenoma, | | Multiple | X X X | Alveolar/Bronchiolar Carcinoma | X X X X | Alveolar/Bronchiolar Carcinoma, | | Multiple | X X X X X X | Granulosa Cell Tumor Malignant, | | Metastatic, Ovary | | Hemangiosarcoma, Metastatic, Heart | X | Hepatocellular Carcinoma, Metastatic,| | Liver | X | Histiocytic Sarcoma | X X X X | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X | Sarcoma, Metastatic, Skin | X | Mediastinum, Alveolar/Bronchiolar | | Carcinoma, Metastatic, Lung | | Mediastinum, Hemangiosarcoma, | | Metastatic, Heart | X X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | + | __________________________________________________________________________| Eye | + | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + M + + + + + + + | Adenocarcinoma | | Adenoma | X X X X X | Bilateral, Adenoma | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + | Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | X X X | Leiomyosarcoma, Metastatic, Stomach | | Leukemia Granulocytic | | _____________________________________________________________________________________________________________________| Page 70 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 3| 5| 5| 4| 4| 6| 5| 4| 4| 4| 3| 4| 4| 4| 2| 4| 5| 5| 6| 0| 2| 7| 5| 2| DAY ON TEST | 8| 5| 6| 6| 5| 5| 6| 0| 7| 8| 6| 7| 0| 1| 5| 0| 5| 2| 3| 2| 0| 8| 0| 0| 8| | 2| 3| 9| 9| 7| 7| 3| 2| 2| 5| 4| 3| 8| 8| 7| 5| 7| 4| 2| 4| 9| 2| 4| 9| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 200 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| URINARY SYSTEM - cont | | | | Lymphoma Malignant Lymphocytic | X | Renal Tubule, Adenoma | X X | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + A + + | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X X X | Leukemia Granulocytic | | Lymphoma Malignant Lymphocytic | X X X X | Lymphoma Malignant Mixed | | _____________________________________________________________________________________________________________________| Page 71 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 2| 6| 3| 4| 2| 3| 5| 5| 2| 5| 2| 4| 3| 3| 3| | | DAY ON TEST | 8| 3| 4| 5| 7| 8| 9| 7| 7| 8| 8| 4| 0| 6| 6| 8| 8| 4| 1| 4| | | | 4| 6| 6| 7| 0| 2| 5| 0| 8| 2| 6| 3| 9| 8| 9| 2| 9| 3| 7| 4| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | A | 200 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + M M + + + | 58 | __________________________________________________________________________|____________| Gallbladder | + A + + + + + + + A + + + + A + A | 49 | __________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + + + + + + + + | 59 | __________________________________________________________________________|____________| Intestine Large, Cecum | + A + + + + + + + + + + + + + + + | 57 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + | 58 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + | 58 | __________________________________________________________________________|____________| Intestine Small | + + + + + + + + + A + + + + + + + | 58 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + M A + + + + A + + | 53 | Histiocytic Sarcoma | | 1 | Leukemia Granulocytic | X | 1 | __________________________________________________________________________|____________| Intestine Small, Ileum | + A + + + + + + + A + + + + + + + | 55 | Leiomyosarcoma, Metastatic, Stomach | | 1 | Lymphoma Malignant Lymphocytic | | 2 | __________________________________________________________________________|____________| Intestine Small, Jejunum | A A + + + + + + + A + + + + A + + | 54 | Histiocytic Sarcoma | | 1 | Leiomyosarcoma, Metastatic, Stomach | | 1 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + | 61 | Hemangiosarcoma, Multiple | X | 1 | Hemangiosarcoma, Metastatic, Heart | X X | 10 | Hepatocellular Carcinoma | X | 8 | Hepatocellular Carcinoma, Multiple | | 1 | Hepatocellular Adenoma | X X | 10 | Hepatocellular Adenoma, Multiple | X | 5 | Histiocytic Sarcoma | X X | 7 | Leiomyosarcoma, Metastatic, Stomach | | 1 | Leukemia Granulocytic | X | 1 | Lymphoma Malignant Lymphocytic | X X | 6 | __________________________________________________________________________|____________| Mesentery | + + | 15 | Fat, Hemangiosarcoma, Metastatic, | | | Heart | X | 2 | Fat, Histiocytic Sarcoma | | 2 | Fat, Leiomyosarcoma, Metastatic, | | | Stomach | | 1 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + | 59 | Acinus, Leiomyosarcoma, Metastatic, | | | Stomach | | 1 | Acinus, Leukemia Granulocytic | X | 1 | Acinus, Lymphoma Malignant | | | Lymphocytic | X | 4 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 72 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 2| 6| 3| 4| 2| 3| 5| 5| 2| 5| 2| 4| 3| 3| 3| | | DAY ON TEST | 8| 3| 4| 5| 7| 8| 9| 7| 7| 8| 8| 4| 0| 6| 6| 8| 8| 4| 1| 4| | | | 4| 6| 6| 7| 0| 2| 5| 0| 8| 2| 6| 3| 9| 8| 9| 2| 9| 3| 7| 4| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | A | 200 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + | 60 | Leukemia Granulocytic | X | 1 | Lymphoma Malignant Lymphocytic | X | 3 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + | 70 | Leiomyosarcoma | | 1 | Leukemia Granulocytic | X | 1 | Lymphoma Malignant Lymphocytic | X | 2 | Squamous Cell Carcinoma | | 1 | Squamous Cell Papilloma | X | 4 | Squamous Cell Papilloma, Multiple | | 1 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + | 68 | Lymphoma Malignant Lymphocytic | X X | 4 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + | 70 | Hemangiosarcoma | X X X X X X X | 22 | Leukemia Granulocytic | X | 1 | Lymphoma Malignant Lymphocytic | X | 2 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + + + + | 60 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + | 60 | Adenoma | X | 3 | Histiocytic Sarcoma | | 3 | Leiomyosarcoma, Metastatic, Stomach | | 1 | Leukemia Granulocytic | X | 1 | Lymphoma Malignant Lymphocytic | X | 2 | Sarcoma, Metastatic, Skin | | 1 | __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + | 60 | Histiocytic Sarcoma | | 1 | Leiomyosarcoma, Metastatic, Stomach | | 1 | Leukemia Granulocytic | X | 1 | Pheochromocytoma Benign | X | 2 | Sarcoma, Metastatic, Skin | | 1 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + | 59 | Adenoma | | 2 | __________________________________________________________________________|____________| Parathyroid Gland | + I + + M + + + + + M + + + + + + | 47 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + M | 58 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 73 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 2| 6| 3| 4| 2| 3| 5| 5| 2| 5| 2| 4| 3| 3| 3| | | DAY ON TEST | 8| 3| 4| 5| 7| 8| 9| 7| 7| 8| 8| 4| 0| 6| 6| 8| 8| 4| 1| 4| | | | 4| 6| 6| 7| 0| 2| 5| 0| 8| 2| 6| 3| 9| 8| 9| 2| 9| 3| 7| 4| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | A | 200 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | Lymphoma Malignant Lymphocytic | X | 2 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + | 59 | Follicular Cell, Adenoma | | 2 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + | 70 | Adenoma | X | 1 | Granulosa Cell Tumor Malignant | X | 3 | Granulosa Cell Tumor Benign | X X X | 9 | Histiocytic Sarcoma | | 3 | Leiomyosarcoma, Metastatic, Stomach | | 1 | Leukemia Granulocytic | X | 1 | Lymphoma Malignant Lymphocytic | X | 4 | Mixed Tumor Benign | | 6 | Bilateral, Granulosa Cell Tumor | | | Benign | | 1 | __________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + | 70 | Adenocarcinoma | X | 1 | Hemangioma | | 1 | Histiocytic Sarcoma | | 1 | Leukemia Granulocytic | X | 1 | Lymphoma Malignant Lymphocytic | | 1 | Polyp Stromal | X | 1 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Blood | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + | 70 | Histiocytic Sarcoma | X X | 4 | Leukemia Granulocytic | X | 1 | Lymphoma Malignant Lymphocytic | X X | 5 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + + + + + | 69 | Axillary, Lymphoma Malignant | | | Lymphocytic | | 2 | Axillary, Sarcoma, Metastatic, Skin | | 1 | Inguinal, Lymphoma Malignant | | | Lymphocytic | | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 74 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 2| 6| 3| 4| 2| 3| 5| 5| 2| 5| 2| 4| 3| 3| 3| | | DAY ON TEST | 8| 3| 4| 5| 7| 8| 9| 7| 7| 8| 8| 4| 0| 6| 6| 8| 8| 4| 1| 4| | | | 4| 6| 6| 7| 0| 2| 5| 0| 8| 2| 6| 3| 9| 8| 9| 2| 9| 3| 7| 4| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | A | 200 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Pancreatic, Histiocytic Sarcoma | X | 1 | Pancreatic, Leiomyosarcoma, | | | Metastatic, Stomach | | 1 | __________________________________________________________________________|____________| Lymph Node, Bronchial | + M + M M + + M + + M M + + + M + + + + | 46 | Adenocarcinoma, Metastatic, Lung | | 1 | Alveolar/Bronchiolar Carcinoma, | | | Metastatic, Lung | X | 1 | Histiocytic Sarcoma | | 3 | Lymphoma Malignant Lymphocytic | X | 4 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + M + + + + M + + M I M + + + M + + | 54 | Histiocytic Sarcoma | X | 2 | Leukemia Granulocytic | X | 1 | Lymphoma Malignant Lymphocytic | X X | 5 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | + + + + + M M + + M + + + + + M + M + M | 51 | Alveolar/Bronchiolar Carcinoma, | | | Metastatic, Lung | X X | 2 | Histiocytic Sarcoma | | 3 | Leukemia Granulocytic | X | 1 | Lymphoma Malignant Lymphocytic | X X | 7 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + | 66 | Histiocytic Sarcoma | X | 6 | Leiomyosarcoma, Metastatic, Stomach | | 1 | Leukemia Granulocytic | X | 1 | Lymphoma Malignant Lymphocytic | X X | 6 | Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + | 70 | Histiocytic Sarcoma | X | 4 | Leukemia Granulocytic | X | 1 | Lymphoma Malignant Lymphocytic | X X | 6 | __________________________________________________________________________|____________| Thymus | + + + + M + + + M + M + + + + + I + + + | 59 | Leukemia Granulocytic | X | 1 | Lymphoma Malignant Lymphocytic | X X | 6 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | + + + + + + + + M + + + + + + + + | 58 | Adenoacanthoma | X X | 4 | Adenocarcinoma | X X | 12 | Adenocarcinoma, Multiple | | 1 | Lymphoma Malignant Lymphocytic | X | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 75 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 2| 6| 3| 4| 2| 3| 5| 5| 2| 5| 2| 4| 3| 3| 3| | | DAY ON TEST | 8| 3| 4| 5| 7| 8| 9| 7| 7| 8| 8| 4| 0| 6| 6| 8| 8| 4| 1| 4| | | | 4| 6| 6| 7| 0| 2| 5| 0| 8| 2| 6| 3| 9| 8| 9| 2| 9| 3| 7| 4| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | A | 200 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ INTEGUMENTARY SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + | 60 | Leiomyosarcoma, Metastatic, Stomach | | 1 | Sebaceous Gland, Adenoma | | 1 | Subcutaneous Tissue, Hemangiosarcoma | | 2 | Subcutaneous Tissue, Sarcoma | | 3 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + | 60 | __________________________________________________________________________|____________| Skeletal Muscle | | 2 | Diaphragm, Sarcoma, Metastatic, Skin | | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + | 60 | Hemangiosarcoma, Metastatic, Heart | X | 2 | Histiocytic Sarcoma | X X | 4 | Lymphoma Malignant Lymphocytic | X | 3 | __________________________________________________________________________|____________| Spinal Cord | | 1 | Lymphoma Malignant Lymphocytic | | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + + + + + + + + + + | 59 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + | 70 | Adenocarcinoma, Multiple | | 1 | Adenocarcinoma, Metastatic, | | | Harderian Gland | X | 1 | Adenocarcinoma, Metastatic, Mammary | | | Gland | X X | 3 | Alveolar/Bronchiolar Adenoma | X X X X X | 13 | Alveolar/Bronchiolar Adenoma, | | | Multiple | | 5 | Alveolar/Bronchiolar Carcinoma | X | 9 | Alveolar/Bronchiolar Carcinoma, | | | Multiple | X X X | 10 | Granulosa Cell Tumor Malignant, | | | Metastatic, Ovary | | 1 | Hemangiosarcoma, Metastatic, Heart | X | 6 | Hepatocellular Carcinoma, Metastatic,| | | Liver | | 3 | Histiocytic Sarcoma | X X | 7 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 76 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 2| 6| 3| 4| 2| 3| 5| 5| 2| 5| 2| 4| 3| 3| 3| | | DAY ON TEST | 8| 3| 4| 5| 7| 8| 9| 7| 7| 8| 8| 4| 0| 6| 6| 8| 8| 4| 1| 4| | | | 4| 6| 6| 7| 0| 2| 5| 0| 8| 2| 6| 3| 9| 8| 9| 2| 9| 3| 7| 4| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | A | 200 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - cont | | | | | | Leukemia Granulocytic | X | 1 | Lymphoma Malignant Lymphocytic | X X | 5 | Sarcoma, Metastatic, Skin | | 1 | Mediastinum, Alveolar/Bronchiolar | | | Carcinoma, Metastatic, Lung | X X | 2 | Mediastinum, Hemangiosarcoma, | | | Metastatic, Heart | | 2 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + | 60 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + | 60 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Ear | + | 2 | __________________________________________________________________________|____________| Eye | + | 3 | __________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + | 60 | Adenocarcinoma | X | 1 | Adenoma | X X X X X X X | 21 | Bilateral, Adenoma | | 2 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + | 60 | Hemangiosarcoma, Metastatic, Heart | | 1 | Histiocytic Sarcoma | X | 5 | Leiomyosarcoma, Metastatic, Stomach | | 1 | Leukemia Granulocytic | X | 1 | Lymphoma Malignant Lymphocytic | X X | 5 | Renal Tubule, Adenoma | | 2 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + | 59 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X | 4 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + | 70 | Histiocytic Sarcoma | X X | 7 | Leukemia Granulocytic | X | 1 | Lymphoma Malignant Lymphocytic | X X X | 9 | Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 77 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 3| 2| 3| 2| 2| 3| 2| 3| 3| 3| 3| 2| 3| 2| 3| 3| 2| 3| 3| 4| 3| 2| 3| 2| 2| DAY ON TEST | 5| 4| 0| 9| 7| 1| 8| 9| 2| 1| 1| 8| 3| 8| 2| 0| 8| 8| 1| 4| 7| 0| 8| 0| 8| | 3| 0| 7| 4| 5| 7| 2| 8| 8| 7| 9| 2| 1| 0| 9| 7| 2| 7| 9| 3| 9| 5| 5| 3| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 625 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | A + + A A + + + + + A M + A + + + + + + + M + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Intestine Large | + + + + A + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + A A + + + + + + + + A + + + + + + + + + + +| Histiocytic Sarcoma | X | __________________________________________________________________________| Intestine Large, Colon | + + + + A + + + + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + A + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + A A + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | A + + A A + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Intestine Small, Ileum | + + + A A + + + + + + + + A + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Intestine Small, Jejunum | A + + A A + + + + + A + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Mammary | | Gland | | Hemangiosarcoma, Multiple | | Hemangiosarcoma, Metastatic, Heart | X X X X X X | Hemangiosarcoma, Metastatic, Skin | | Hepatocellular Carcinoma | | Hepatocellular Adenoma | | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X X X X X X X| Lymphoma Malignant | | Sarcoma, Metastatic, Mammary Gland | X | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Mesentery | + + + | Sarcoma, Metastatic, Mammary Gland | X | Fat, Hemangiosarcoma, Metastatic, | | Heart | X | Fat, Hemangiosarcoma, Metastatic, | | Spleen | | Fat, Sarcoma, Metastatic, Stomach | X | Fat, Squamous Cell Carcinoma, | | Metastatic, Stomach | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Sarcoma, Metastatic, Mammary Gland | X | Acinus, Lymphoma Malignant | | Lymphocytic | X X X X| Acinus, Squamous Cell Carcinoma, | | Metastatic, Stomach | | _____________________________________________________________________________________________________________________| Page 78 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 3| 2| 3| 2| 2| 3| 2| 3| 3| 3| 3| 2| 3| 2| 3| 3| 2| 3| 3| 4| 3| 2| 3| 2| 2| DAY ON TEST | 5| 4| 0| 9| 7| 1| 8| 9| 2| 1| 1| 8| 3| 8| 2| 0| 8| 8| 1| 4| 7| 0| 8| 0| 8| | 3| 0| 7| 4| 5| 7| 2| 8| 8| 7| 9| 2| 1| 0| 9| 7| 2| 7| 9| 3| 9| 5| 5| 3| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 625 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM - cont | | | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X X | Sarcoma | X | Sarcoma, Metastatic, Mammary Gland | X | Squamous Cell Carcinoma | X X | Squamous Cell Papilloma | X X | Squamous Cell Papilloma, Multiple | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X | Sarcoma, Metastatic, Mammary Gland | X | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Blood Vessel | + | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X X X X X X X X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X | Sarcoma, Metastatic, Mammary Gland | X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Mammary | | Gland | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X | Sarcoma, Metastatic, Stomach | X | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Mammary | | Gland | | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | + + + + + + M M + + M + + + + + + + M M + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Pituitary Gland | + + + + M + M M + + I + + + + + + + + + + + + M +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| Page 79 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 3| 2| 3| 2| 2| 3| 2| 3| 3| 3| 3| 2| 3| 2| 3| 3| 2| 3| 3| 4| 3| 2| 3| 2| 2| DAY ON TEST | 5| 4| 0| 9| 7| 1| 8| 9| 2| 1| 1| 8| 3| 8| 2| 0| 8| 8| 1| 4| 7| 0| 8| 0| 8| | 3| 0| 7| 4| 5| 7| 2| 8| 8| 7| 9| 2| 1| 0| 9| 7| 2| 7| 9| 3| 9| 5| 5| 3| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 625 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Follicular Cell, Adenoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | Granulosa Cell Tumor Benign | X X X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X| Mixed Tumor Benign | X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X | Polyp Stromal | X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X X X X X X X| __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + I + + +| Histiocytic Sarcoma | | Axillary, Lymphoma Malignant | | Lymphocytic | X X | Deep Cervical, Lymphoma Malignant | | Lymphocytic | X | Inguinal, Lymphoma Malignant | | Lymphocytic | X | Renal, Lymphoma Malignant Lymphocytic| X | __________________________________________________________________________| Lymph Node, Bronchial | + + + + + M + M + M + + + M + + + M M M M M M + +| Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X| Lymphoma Malignant | | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + M + + + + + + M + + I + + I + + I + + +| Adenocarcinoma, Metastatic, Mammary | | Gland | | Lymphoma Malignant Lymphocytic | X X X X X X X| Lymphoma Malignant | | __________________________________________________________________________| Lymph Node, Mediastinal | + + + + + + + + M + + + + M + + + + M + + M + + +| Alveolar/Bronchiolar Carcinoma, | | _____________________________________________________________________________________________________________________| Page 80 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 3| 2| 3| 2| 2| 3| 2| 3| 3| 3| 3| 2| 3| 2| 3| 3| 2| 3| 3| 4| 3| 2| 3| 2| 2| DAY ON TEST | 5| 4| 0| 9| 7| 1| 8| 9| 2| 1| 1| 8| 3| 8| 2| 0| 8| 8| 1| 4| 7| 0| 8| 0| 8| | 3| 0| 7| 4| 5| 7| 2| 8| 8| 7| 9| 2| 1| 0| 9| 7| 2| 7| 9| 3| 9| 5| 5| 3| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 625 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Metastatic, Lung | X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X| Lymphoma Malignant | | Sarcoma, Metastatic, Mammary Gland | X | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + I + + + + + + + M M + + +| Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X X X X X X| Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X X X X X X X| __________________________________________________________________________| Thymus | + + + + + + + I + + + + M + + + + + + + M + + + +| Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X X X| Lymphoma Malignant | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + M + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X X X X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | Mixed Tumor Malignant | X | Sarcoma | X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Keratoacanthoma | X | Lymphoma Malignant Lymphocytic | X | Subcutaneous Tissue, Hemangiosarcoma | X | Subcutaneous Tissue, Hemangiosarcoma,| | Metastatic, Heart | X | Subcutaneous Tissue, | | Neurofibrosarcoma | | Subcutaneous Tissue, Sarcoma | X | Subcutaneous Tissue, Sarcoma, | | Multiple | | Subcutaneous Tissue, Squamous Cell | | Carcinoma, Metastatic, Stomach | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | _____________________________________________________________________________________________________________________| Page 81 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 3| 2| 3| 2| 2| 3| 2| 3| 3| 3| 3| 2| 3| 2| 3| 3| 2| 3| 3| 4| 3| 2| 3| 2| 2| DAY ON TEST | 5| 4| 0| 9| 7| 1| 8| 9| 2| 1| 1| 8| 3| 8| 2| 0| 8| 8| 1| 4| 7| 0| 8| 0| 8| | 3| 0| 7| 4| 5| 7| 2| 8| 8| 7| 9| 2| 1| 0| 9| 7| 2| 7| 9| 3| 9| 5| 5| 3| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 625 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM - cont | | | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Vertebra, Lymphoma Malignant | | Lymphocytic | | __________________________________________________________________________| Skeletal Muscle | + | Diaphragm, Sarcoma, Metastatic, | | Stomach | X | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X| __________________________________________________________________________| Spinal Cord | + + +| Lymphoma Malignant Lymphocytic | X X X| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Mammary | | Gland | X | Alveolar/Bronchiolar Adenoma | X X X X X X X | Alveolar/Bronchiolar Adenoma, | | Multiple | X | Alveolar/Bronchiolar Carcinoma | X X X | Alveolar/Bronchiolar Carcinoma, | | Multiple | X | Hemangiosarcoma, Metastatic, Heart | X X X X X | Hemangiosarcoma, Metastatic, Skin | | Hepatocellular Carcinoma, Metastatic,| | Liver | | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X X X | Mixed Tumor Malignant, Metastatic, | | Mammary Gland | X | Sarcoma, Metastatic, Skin | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | Mediastinum, Alveolar/Bronchiolar | | Carcinoma, Metastatic, Lung | X | Mediastinum, Hemangiosarcoma, | | Metastatic, Heart | | Mediastinum, Mixed Tumor Malignant, | | Metastatic, Mammary Gland | X | _____________________________________________________________________________________________________________________| Page 82 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 3| 2| 3| 2| 2| 3| 2| 3| 3| 3| 3| 2| 3| 2| 3| 3| 2| 3| 3| 4| 3| 2| 3| 2| 2| DAY ON TEST | 5| 4| 0| 9| 7| 1| 8| 9| 2| 1| 1| 8| 3| 8| 2| 0| 8| 8| 1| 4| 7| 0| 8| 0| 8| | 3| 0| 7| 4| 5| 7| 2| 8| 8| 7| 9| 2| 1| 0| 9| 7| 2| 7| 9| 3| 9| 5| 5| 3| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 625 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Mediastinum, Sarcoma, Metastatic, | | Skin | | Mediastinum, Squamous Cell Carcinoma,| | Metastatic, Stomach | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Trachea | + + + A + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | | __________________________________________________________________________| Harderian Gland | + M + + + + + + + + + I + + + + + + + + + M + M +| Adenoma | X X | __________________________________________________________________________| Zymbal's Gland | | Adenoma | | Carcinoma | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | | Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X X X X| Lymphoma Malignant | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Urinary Bladder | + + + M + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X | Squamous Cell Carcinoma, Metastatic, | | Stomach | | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X | Lymphoma Malignant | | Lymphoma Malignant Lymphocytic | X X X X X X X X| _____________________________________________________________________________________________________________________| Page 83 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 1| 3| 3| 2| 2| 3| 2| 4| 3| 3| 3| 3| 2| 3| 3| 2| 3| 4| 2| 3| 3| 2| 2| 2| DAY ON TEST | 5| 8| 3| 2| 8| 8| 6| 9| 4| 1| 3| 8| 7| 3| 9| 5| 2| 0| 5| 5| 7| 6| 8| 8| 7| | 9| 9| 5| 5| 2| 9| 9| 8| 1| 1| 5| 4| 1| 0| 7| 0| 2| 0| 0| 7| 6| 6| 5| 3| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 625 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + A + + + I A + + I + + + A + A + + A + A A A + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Intestine Large | + A + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + A + + + A + + + A + + + A + + + + + + + A + + +| Histiocytic Sarcoma | | __________________________________________________________________________| Intestine Large, Colon | + A + + + + A + + + + + + + + + + + + + + A + + +| __________________________________________________________________________| Intestine Large, Rectum | + M + + + + A + + + + + + + + + + M + + + + + + +| __________________________________________________________________________| Intestine Small | + A + + + A + + + A + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + A + + + A + + + A + + + + + + + + M + + A A + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Intestine Small, Ileum | + A + + + A + + + A + + + A + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Intestine Small, Jejunum | + A + + + A M + + A + A + A + A + + A + + A + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Mammary | | Gland | | Hemangiosarcoma, Multiple | X | Hemangiosarcoma, Metastatic, Heart | X X X X X X | Hemangiosarcoma, Metastatic, Skin | | Hepatocellular Carcinoma | | Hepatocellular Adenoma | X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X X X X| Lymphoma Malignant | X | Sarcoma, Metastatic, Mammary Gland | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Mesentery | | Sarcoma, Metastatic, Mammary Gland | | Fat, Hemangiosarcoma, Metastatic, | | Heart | | Fat, Hemangiosarcoma, Metastatic, | | Spleen | | Fat, Sarcoma, Metastatic, Stomach | | Fat, Squamous Cell Carcinoma, | | Metastatic, Stomach | | __________________________________________________________________________| Pancreas | + A + + + + + + + + + + + + + + + + + + + + + + +| Sarcoma, Metastatic, Mammary Gland | | Acinus, Lymphoma Malignant | | Lymphocytic | X | Acinus, Squamous Cell Carcinoma, | | Metastatic, Stomach | | _____________________________________________________________________________________________________________________| Page 84 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 1| 3| 3| 2| 2| 3| 2| 4| 3| 3| 3| 3| 2| 3| 3| 2| 3| 4| 2| 3| 3| 2| 2| 2| DAY ON TEST | 5| 8| 3| 2| 8| 8| 6| 9| 4| 1| 3| 8| 7| 3| 9| 5| 2| 0| 5| 5| 7| 6| 8| 8| 7| | 9| 9| 5| 5| 2| 9| 9| 8| 1| 1| 5| 4| 1| 0| 7| 0| 2| 0| 0| 7| 6| 6| 5| 3| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 625 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM - cont | | | | __________________________________________________________________________| Salivary Glands | + A + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Stomach | + A + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + A + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X | Sarcoma | | Sarcoma, Metastatic, Mammary Gland | | Squamous Cell Carcinoma | X| Squamous Cell Papilloma | X X X X X | Squamous Cell Papilloma, Multiple | | __________________________________________________________________________| Stomach, Glandular | + A + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X | Sarcoma, Metastatic, Mammary Gland | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Blood Vessel | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X X X X X X X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X| Sarcoma, Metastatic, Mammary Gland | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + A + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + A + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Mammary | | Gland | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X | Sarcoma, Metastatic, Stomach | | __________________________________________________________________________| Adrenal Gland, Medulla | + A + + + + + + + + + + + A + + + + + + + + + + +| Adenocarcinoma, Metastatic, Mammary | | Gland | | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Islets, Pancreatic | + A + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | M + + + + + + + + + + + + + + + + + + + M + + + +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Pituitary Gland | I + + + + + + + + + + + + I + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X X X| __________________________________________________________________________| Thyroid Gland | + A + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| Page 85 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 1| 3| 3| 2| 2| 3| 2| 4| 3| 3| 3| 3| 2| 3| 3| 2| 3| 4| 2| 3| 3| 2| 2| 2| DAY ON TEST | 5| 8| 3| 2| 8| 8| 6| 9| 4| 1| 3| 8| 7| 3| 9| 5| 2| 0| 5| 5| 7| 6| 8| 8| 7| | 9| 9| 5| 5| 2| 9| 9| 8| 1| 1| 5| 4| 1| 0| 7| 0| 2| 0| 0| 7| 6| 6| 5| 3| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 625 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Follicular Cell, Adenoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Ovary | + A + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Granulosa Cell Tumor Benign | X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X| Mixed Tumor Benign | | __________________________________________________________________________| Uterus | + A + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | Polyp Stromal | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + A + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X| __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Axillary, Lymphoma Malignant | | Lymphocytic | X X X | Deep Cervical, Lymphoma Malignant | | Lymphocytic | X | Inguinal, Lymphoma Malignant | | Lymphocytic | X X | Renal, Lymphoma Malignant Lymphocytic| X | __________________________________________________________________________| Lymph Node, Bronchial | + + + + + + M + + M + + + + + + + + + + M M + + +| Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X| Lymphoma Malignant | X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + M + M + M M I M + + + + + + + + + + + M +| Adenocarcinoma, Metastatic, Mammary | | Gland | | Lymphoma Malignant Lymphocytic | X X X X X X X | Lymphoma Malignant | X | __________________________________________________________________________| Lymph Node, Mediastinal | + + + + + M + + + + + M + + + M M + + + + + + + +| Alveolar/Bronchiolar Carcinoma, | | _____________________________________________________________________________________________________________________| Page 86 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 1| 3| 3| 2| 2| 3| 2| 4| 3| 3| 3| 3| 2| 3| 3| 2| 3| 4| 2| 3| 3| 2| 2| 2| DAY ON TEST | 5| 8| 3| 2| 8| 8| 6| 9| 4| 1| 3| 8| 7| 3| 9| 5| 2| 0| 5| 5| 7| 6| 8| 8| 7| | 9| 9| 5| 5| 2| 9| 9| 8| 1| 1| 5| 4| 1| 0| 7| 0| 2| 0| 0| 7| 6| 6| 5| 3| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 625 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Metastatic, Lung | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X X X X| Lymphoma Malignant | X | Sarcoma, Metastatic, Mammary Gland | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Lymph Node, Mesenteric | + A + + + + + + + + + + + + M + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X X | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Spleen | + A + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X X X| __________________________________________________________________________| Thymus | + + + + + + M + + + + + M + + + + + + + + + + + +| Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X X X X X| Lymphoma Malignant | X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + M + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X X X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | Mixed Tumor Malignant | X | Sarcoma | | __________________________________________________________________________| Skin | + A + + + + + + + + + + + + + + + + + + + + + + +| Keratoacanthoma | | Lymphoma Malignant Lymphocytic | | Subcutaneous Tissue, Hemangiosarcoma | | Subcutaneous Tissue, Hemangiosarcoma,| | Metastatic, Heart | | Subcutaneous Tissue, | | Neurofibrosarcoma | | Subcutaneous Tissue, Sarcoma | | Subcutaneous Tissue, Sarcoma, | | Multiple | | Subcutaneous Tissue, Squamous Cell | | Carcinoma, Metastatic, Stomach | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | _____________________________________________________________________________________________________________________| Page 87 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 1| 3| 3| 2| 2| 3| 2| 4| 3| 3| 3| 3| 2| 3| 3| 2| 3| 4| 2| 3| 3| 2| 2| 2| DAY ON TEST | 5| 8| 3| 2| 8| 8| 6| 9| 4| 1| 3| 8| 7| 3| 9| 5| 2| 0| 5| 5| 7| 6| 8| 8| 7| | 9| 9| 5| 5| 2| 9| 9| 8| 1| 1| 5| 4| 1| 0| 7| 0| 2| 0| 0| 7| 6| 6| 5| 3| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 625 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM - cont | | | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Vertebra, Lymphoma Malignant | | Lymphocytic | | __________________________________________________________________________| Skeletal Muscle | | Diaphragm, Sarcoma, Metastatic, | | Stomach | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X | __________________________________________________________________________| Spinal Cord | + + | Lymphoma Malignant Lymphocytic | X X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + A + + + A + + + + + I + + + + I + + + + + + + +| __________________________________________________________________________| Lung | + A + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Mammary | | Gland | X X | Alveolar/Bronchiolar Adenoma | X X X X X X | Alveolar/Bronchiolar Adenoma, | | Multiple | X | Alveolar/Bronchiolar Carcinoma | X | Alveolar/Bronchiolar Carcinoma, | | Multiple | | Hemangiosarcoma, Metastatic, Heart | X X X X X | Hemangiosarcoma, Metastatic, Skin | | Hepatocellular Carcinoma, Metastatic,| | Liver | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X X X| Mixed Tumor Malignant, Metastatic, | | Mammary Gland | X | Sarcoma, Metastatic, Skin | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | Mediastinum, Alveolar/Bronchiolar | | Carcinoma, Metastatic, Lung | | Mediastinum, Hemangiosarcoma, | | Metastatic, Heart | | Mediastinum, Mixed Tumor Malignant, | | Metastatic, Mammary Gland | | _____________________________________________________________________________________________________________________| Page 88 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 1| 3| 3| 2| 2| 3| 2| 4| 3| 3| 3| 3| 2| 3| 3| 2| 3| 4| 2| 3| 3| 2| 2| 2| DAY ON TEST | 5| 8| 3| 2| 8| 8| 6| 9| 4| 1| 3| 8| 7| 3| 9| 5| 2| 0| 5| 5| 7| 6| 8| 8| 7| | 9| 9| 5| 5| 2| 9| 9| 8| 1| 1| 5| 4| 1| 0| 7| 0| 2| 0| 0| 7| 6| 6| 5| 3| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 625 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Mediastinum, Sarcoma, Metastatic, | | Skin | | Mediastinum, Squamous Cell Carcinoma,| | Metastatic, Stomach | | __________________________________________________________________________| Nose | + A + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Trachea | + A + + + A + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | +| __________________________________________________________________________| Harderian Gland | I I + + + + + + + + + + + M + + M + + M + + + + +| Adenoma | X | __________________________________________________________________________| Zymbal's Gland | + | Adenoma | X | Carcinoma | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | | Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X X X| Lymphoma Malignant | X | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Urinary Bladder | + A + + + + + + + + + + + A + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X X | Squamous Cell Carcinoma, Metastatic, | | Stomach | | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant | X | Lymphoma Malignant Lymphocytic | X X X X X X X X X X| _____________________________________________________________________________________________________________________| Page 89 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 3| 3| 3| 4| 2| 2| 2| 0| 2| 2| 3| 2| 3| 2| 2| 2| 4| 3| 2| 4| 4| 2| 3| 3| DAY ON TEST | 0| 8| 4| 3| 5| 8| 7| 2| 1| 8| 8| 9| 8| 0| 4| 8| 8| 2| 5| 5| 2| 1| 9| 2| 7| | 1| 7| 1| 0| 7| 5| 2| 1| 1| 2| 2| 2| 0| 0| 6| 2| 2| 5| 6| 3| 5| 8| 6| 9| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 625 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + M + + + + + + + + A + + + + + A A + + A A + A| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + A + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + A + + + A A + +| Histiocytic Sarcoma | | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + A + + + + A + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + A A + +| __________________________________________________________________________| Intestine Small | + + A + + + + + + + + + + + + + + + + + + A A + A| __________________________________________________________________________| Intestine Small, Duodenum | + + A + + + + + + + + M + + + + + A A + + A A + A| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Intestine Small, Ileum | + + A + + + + + + M + + + + + + + A + + + A A + A| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Intestine Small, Jejunum | + + A + + + + + + + + A + + + + + + + A + A A + A| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Mammary | | Gland | X | Hemangiosarcoma, Multiple | | Hemangiosarcoma, Metastatic, Heart | X X X| Hemangiosarcoma, Metastatic, Skin | X | Hepatocellular Carcinoma | X | Hepatocellular Adenoma | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant | | Sarcoma, Metastatic, Mammary Gland | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Mesentery | + + | Sarcoma, Metastatic, Mammary Gland | | Fat, Hemangiosarcoma, Metastatic, | | Heart | | Fat, Hemangiosarcoma, Metastatic, | | Spleen | X | Fat, Sarcoma, Metastatic, Stomach | | Fat, Squamous Cell Carcinoma, | | Metastatic, Stomach | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + A + +| Sarcoma, Metastatic, Mammary Gland | | Acinus, Lymphoma Malignant | | Lymphocytic | | Acinus, Squamous Cell Carcinoma, | | Metastatic, Stomach | | _____________________________________________________________________________________________________________________| Page 90 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 3| 3| 3| 4| 2| 2| 2| 0| 2| 2| 3| 2| 3| 2| 2| 2| 4| 3| 2| 4| 4| 2| 3| 3| DAY ON TEST | 0| 8| 4| 3| 5| 8| 7| 2| 1| 8| 8| 9| 8| 0| 4| 8| 8| 2| 5| 5| 2| 1| 9| 2| 7| | 1| 7| 1| 0| 7| 5| 2| 1| 1| 2| 2| 2| 0| 0| 6| 2| 2| 5| 6| 3| 5| 8| 6| 9| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 625 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM - cont | | | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | Sarcoma | | Sarcoma, Metastatic, Mammary Gland | | Squamous Cell Carcinoma | X | Squamous Cell Papilloma | X X X X X| Squamous Cell Papilloma, Multiple | X | __________________________________________________________________________| Stomach, Glandular | + + + M + + + + + + + + + + + + + + + + + + A + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | | Sarcoma, Metastatic, Mammary Gland | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Blood Vessel | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X X X X X| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X | Sarcoma, Metastatic, Mammary Gland | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + A + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + A + +| Adenocarcinoma, Metastatic, Mammary | | Gland | X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X | Sarcoma, Metastatic, Stomach | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + M + + + + + + + A + +| Adenocarcinoma, Metastatic, Mammary | | Gland | X | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + A + +| __________________________________________________________________________| Parathyroid Gland | + M + + + + + + + + + + + + + M + + M + + M + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Pituitary Gland | + + + + + + I + + + + + + + + + + + + + + + A + +| Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + A + +| Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| Page 91 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 3| 3| 3| 4| 2| 2| 2| 0| 2| 2| 3| 2| 3| 2| 2| 2| 4| 3| 2| 4| 4| 2| 3| 3| DAY ON TEST | 0| 8| 4| 3| 5| 8| 7| 2| 1| 8| 8| 9| 8| 0| 4| 8| 8| 2| 5| 5| 2| 1| 9| 2| 7| | 1| 7| 1| 0| 7| 5| 2| 1| 1| 2| 2| 2| 0| 0| 6| 2| 2| 5| 6| 3| 5| 8| 6| 9| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 625 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Follicular Cell, Adenoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | Granulosa Cell Tumor Benign | X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X | Mixed Tumor Benign | | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + A + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | Polyp Stromal | X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | M | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Axillary, Lymphoma Malignant | | Lymphocytic | | Deep Cervical, Lymphoma Malignant | | Lymphocytic | | Inguinal, Lymphoma Malignant | | Lymphocytic | X | Renal, Lymphoma Malignant Lymphocytic| | __________________________________________________________________________| Lymph Node, Bronchial | M + + + + M + + + + + + M + + M M M + + + + + + M| Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X X | Lymphoma Malignant | | __________________________________________________________________________| Lymph Node, Mandibular | + M + + + + + M + M + M + + + + M M + + M + A + M| Adenocarcinoma, Metastatic, Mammary | | Gland | | Lymphoma Malignant Lymphocytic | X X X X | Lymphoma Malignant | | __________________________________________________________________________| Lymph Node, Mediastinal | + + + + M + M + + + M + + M + M + + + + + + + + +| Alveolar/Bronchiolar Carcinoma, | | _____________________________________________________________________________________________________________________| Page 92 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 3| 3| 3| 4| 2| 2| 2| 0| 2| 2| 3| 2| 3| 2| 2| 2| 4| 3| 2| 4| 4| 2| 3| 3| DAY ON TEST | 0| 8| 4| 3| 5| 8| 7| 2| 1| 8| 8| 9| 8| 0| 4| 8| 8| 2| 5| 5| 2| 1| 9| 2| 7| | 1| 7| 1| 0| 7| 5| 2| 1| 1| 2| 2| 2| 0| 0| 6| 2| 2| 5| 6| 3| 5| 8| 6| 9| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 625 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Metastatic, Lung | X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X | Lymphoma Malignant | | Sarcoma, Metastatic, Mammary Gland | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + I + + A + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + A + +| Hemangiosarcoma | X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X | __________________________________________________________________________| Thymus | + + + + + M + + + + + + + + + + + + + + + + A + +| Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X | Lymphoma Malignant | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X X X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X | Mixed Tumor Malignant | X | Sarcoma | | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Keratoacanthoma | | Lymphoma Malignant Lymphocytic | | Subcutaneous Tissue, Hemangiosarcoma | X | Subcutaneous Tissue, Hemangiosarcoma,| | Metastatic, Heart | X| Subcutaneous Tissue, | | Neurofibrosarcoma | | Subcutaneous Tissue, Sarcoma | X | Subcutaneous Tissue, Sarcoma, | | Multiple | | Subcutaneous Tissue, Squamous Cell | | Carcinoma, Metastatic, Stomach | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | _____________________________________________________________________________________________________________________| Page 93 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 3| 3| 3| 4| 2| 2| 2| 0| 2| 2| 3| 2| 3| 2| 2| 2| 4| 3| 2| 4| 4| 2| 3| 3| DAY ON TEST | 0| 8| 4| 3| 5| 8| 7| 2| 1| 8| 8| 9| 8| 0| 4| 8| 8| 2| 5| 5| 2| 1| 9| 2| 7| | 1| 7| 1| 0| 7| 5| 2| 1| 1| 2| 2| 2| 0| 0| 6| 2| 2| 5| 6| 3| 5| 8| 6| 9| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 625 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM - cont | | | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Vertebra, Lymphoma Malignant | | Lymphocytic | X | __________________________________________________________________________| Skeletal Muscle | + | Diaphragm, Sarcoma, Metastatic, | | Stomach | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Spinal Cord | + + + | Lymphoma Malignant Lymphocytic | X X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + I + + + + + + + + + + + + + + + + + + + A + I| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + A + +| Adenocarcinoma, Metastatic, Mammary | | Gland | X | Alveolar/Bronchiolar Adenoma | X X | Alveolar/Bronchiolar Adenoma, | | Multiple | X | Alveolar/Bronchiolar Carcinoma | X | Alveolar/Bronchiolar Carcinoma, | | Multiple | X | Hemangiosarcoma, Metastatic, Heart | X X X X| Hemangiosarcoma, Metastatic, Skin | X | Hepatocellular Carcinoma, Metastatic,| | Liver | X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X | Mixed Tumor Malignant, Metastatic, | | Mammary Gland | X | Sarcoma, Metastatic, Skin | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | Mediastinum, Alveolar/Bronchiolar | | Carcinoma, Metastatic, Lung | X X | Mediastinum, Hemangiosarcoma, | | Metastatic, Heart | X | Mediastinum, Mixed Tumor Malignant, | | Metastatic, Mammary Gland | | _____________________________________________________________________________________________________________________| Page 94 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 3| 3| 3| 4| 2| 2| 2| 0| 2| 2| 3| 2| 3| 2| 2| 2| 4| 3| 2| 4| 4| 2| 3| 3| DAY ON TEST | 0| 8| 4| 3| 5| 8| 7| 2| 1| 8| 8| 9| 8| 0| 4| 8| 8| 2| 5| 5| 2| 1| 9| 2| 7| | 1| 7| 1| 0| 7| 5| 2| 1| 1| 2| 2| 2| 0| 0| 6| 2| 2| 5| 6| 3| 5| 8| 6| 9| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 625 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Mediastinum, Sarcoma, Metastatic, | | Skin | | Mediastinum, Squamous Cell Carcinoma,| | Metastatic, Stomach | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Trachea | + + A + + + + + + + + + + + + + + + + + + + A + A| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + | __________________________________________________________________________| Harderian Gland | + + + + + + + M + + M + + + M + M + + + + + + + +| Adenoma | X X X X | __________________________________________________________________________| Zymbal's Gland | + | Adenoma | | Carcinoma | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | X | Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X | Lymphoma Malignant | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + A + +| Lymphoma Malignant Lymphocytic | X X | Squamous Cell Carcinoma, Metastatic, | | Stomach | | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant | | Lymphoma Malignant Lymphocytic | X X X X X X X | _____________________________________________________________________________________________________________________| Page 95 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 3| 3| 3| 2| 3| 4| 2| 3| 3| 3| 4| 4| 2| 2| 3| | | DAY ON TEST | 6| 1| 5| 5| 1| 1| 3| 7| 1| 8| 5| 1| 3| 8| 6| | | | 7| 6| 7| 7| 6| 8| 0| 6| 5| 5| 7| 8| 2| 0| 4| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | A | 625 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + M + + + | 89 | __________________________________________________________________________|____________| Gallbladder | A + + + + + + + + + + + A + + | 64 | Lymphoma Malignant Lymphocytic | X | 1 | __________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + + + + + + | 87 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + A + + | 78 | Histiocytic Sarcoma | | 1 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + | 83 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + A + + | 83 | __________________________________________________________________________|____________| Intestine Small | A + + + + + + + + + + + + + + | 80 | __________________________________________________________________________|____________| Intestine Small, Duodenum | A + + + + + + + + + + + A + + | 72 | Lymphoma Malignant Lymphocytic | X | 2 | __________________________________________________________________________|____________| Intestine Small, Ileum | A + + + + + + + + + + + A + + | 75 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Intestine Small, Jejunum | A + + + + + + + + + + + + + + | 70 | Lymphoma Malignant Lymphocytic | X | 2 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 90 | Adenocarcinoma, Metastatic, Mammary | | | Gland | | 1 | Hemangiosarcoma, Multiple | | 1 | Hemangiosarcoma, Metastatic, Heart | X X X X X | 20 | Hemangiosarcoma, Metastatic, Skin | | 1 | Hepatocellular Carcinoma | | 1 | Hepatocellular Adenoma | X | 2 | Histiocytic Sarcoma | | 4 | Lymphoma Malignant Lymphocytic | X X X X | 23 | Lymphoma Malignant | | 1 | Sarcoma, Metastatic, Mammary Gland | | 1 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | X X | 2 | __________________________________________________________________________|____________| Mesentery | + | 6 | Sarcoma, Metastatic, Mammary Gland | | 1 | Fat, Hemangiosarcoma, Metastatic, | | | Heart | | 1 | Fat, Hemangiosarcoma, Metastatic, | | | Spleen | | 1 | Fat, Sarcoma, Metastatic, Stomach | | 1 | Fat, Squamous Cell Carcinoma, | | | Metastatic, Stomach | X | 1 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + | 88 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 96 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 3| 3| 3| 2| 3| 4| 2| 3| 3| 3| 4| 4| 2| 2| 3| | | DAY ON TEST | 6| 1| 5| 5| 1| 1| 3| 7| 1| 8| 5| 1| 3| 8| 6| | | | 7| 6| 7| 7| 6| 8| 0| 6| 5| 5| 7| 8| 2| 0| 4| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | A | 625 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM - cont | | | | | | Sarcoma, Metastatic, Mammary Gland | | 1 | Acinus, Lymphoma Malignant | | | Lymphocytic | X X | 7 | Acinus, Squamous Cell Carcinoma, | | | Metastatic, Stomach | X X | 2 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + | 89 | Lymphoma Malignant Lymphocytic | X | 5 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + | 89 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + | 89 | Lymphoma Malignant Lymphocytic | X X | 7 | Sarcoma | | 1 | Sarcoma, Metastatic, Mammary Gland | | 1 | Squamous Cell Carcinoma | X X X | 7 | Squamous Cell Papilloma | X X X | 15 | Squamous Cell Papilloma, Multiple | | 1 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + | 86 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X X | 7 | Sarcoma, Metastatic, Mammary Gland | | 1 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | X X | 3 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Blood Vessel | | 1 | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 90 | Hemangiosarcoma | X X X X X | 25 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X X X | 19 | Sarcoma, Metastatic, Mammary Gland | | 1 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + + | 88 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + | 88 | Adenocarcinoma, Metastatic, Mammary | | | Gland | | 1 | Histiocytic Sarcoma | | 2 | Lymphoma Malignant Lymphocytic | X | 9 | Sarcoma, Metastatic, Stomach | | 1 | __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + | 86 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 97 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 3| 3| 3| 2| 3| 4| 2| 3| 3| 3| 4| 4| 2| 2| 3| | | DAY ON TEST | 6| 1| 5| 5| 1| 1| 3| 7| 1| 8| 5| 1| 3| 8| 6| | | | 7| 6| 7| 7| 6| 8| 0| 6| 5| 5| 7| 8| 2| 0| 4| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | A | 625 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | Adenocarcinoma, Metastatic, Mammary | | | Gland | | 1 | Lymphoma Malignant Lymphocytic | | 2 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + | 88 | __________________________________________________________________________|____________| Parathyroid Gland | + + M M + + + + + + + + + + + | 77 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + I + + + + + + | 80 | Lymphoma Malignant Lymphocytic | X | 8 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + | 88 | Lymphoma Malignant Lymphocytic | X | 2 | Follicular Cell, Adenoma | | 1 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + | 89 | Adenoma | | 1 | Granulosa Cell Tumor Benign | X X | 7 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X X X | 19 | Mixed Tumor Benign | | 1 | __________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + | 88 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X | 9 | Polyp Stromal | X X | 4 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Blood | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + | 89 | Histiocytic Sarcoma | | 2 | Lymphoma Malignant Lymphocytic | X X X X X X X | 31 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + | 89 | Histiocytic Sarcoma | | 1 | Axillary, Lymphoma Malignant | | | Lymphocytic | X | 6 | Deep Cervical, Lymphoma Malignant | | | Lymphocytic | | 2 | Inguinal, Lymphoma Malignant | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 98 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 3| 3| 3| 2| 3| 4| 2| 3| 3| 3| 4| 4| 2| 2| 3| | | DAY ON TEST | 6| 1| 5| 5| 1| 1| 3| 7| 1| 8| 5| 1| 3| 8| 6| | | | 7| 6| 7| 7| 6| 8| 0| 6| 5| 5| 7| 8| 2| 0| 4| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | A | 625 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Lymphocytic | | 4 | Renal, Lymphoma Malignant Lymphocytic| | 2 | __________________________________________________________________________|____________| Lymph Node, Bronchial | M + M M + M M M + + + M + + + | 62 | Alveolar/Bronchiolar Carcinoma, | | | Metastatic, Lung | | 1 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X X X X X | 29 | Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + M I + + + + I + M M + + + + | 64 | Adenocarcinoma, Metastatic, Mammary | | | Gland | X | 1 | Lymphoma Malignant Lymphocytic | X X X X X | 23 | Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | M + + + + M M + + + + + + + + | 74 | Alveolar/Bronchiolar Carcinoma, | | | Metastatic, Lung | | 2 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X X X X X X X | 29 | Lymphoma Malignant | | 1 | Sarcoma, Metastatic, Mammary Gland | | 1 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | X X | 2 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + M M + + + + + + + | 81 | Histiocytic Sarcoma | | 4 | Lymphoma Malignant Lymphocytic | X X X X X X | 24 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | X | 1 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + | 88 | Hemangiosarcoma | | 1 | Histiocytic Sarcoma | | 4 | Lymphoma Malignant Lymphocytic | X X X X X X X | 27 | __________________________________________________________________________|____________| Thymus | + M M + + + + + + + + + + + + | 81 | Alveolar/Bronchiolar Carcinoma, | | | Metastatic, Lung | | 1 | Histiocytic Sarcoma | | 3 | Lymphoma Malignant Lymphocytic | X X X X X X X | 31 | Lymphoma Malignant | | 1 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 99 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 3| 3| 3| 2| 3| 4| 2| 3| 3| 3| 4| 4| 2| 2| 3| | | DAY ON TEST | 6| 1| 5| 5| 1| 1| 3| 7| 1| 8| 5| 1| 3| 8| 6| | | | 7| 6| 7| 7| 6| 8| 0| 6| 5| 5| 7| 8| 2| 0| 4| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | A | 625 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ INTEGUMENTARY SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + M + + + | 87 | Adenocarcinoma | X X X | 13 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X | 5 | Mixed Tumor Malignant | X | 4 | Sarcoma | | 1 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + | 89 | Keratoacanthoma | | 1 | Lymphoma Malignant Lymphocytic | | 1 | Subcutaneous Tissue, Hemangiosarcoma | | 2 | Subcutaneous Tissue, Hemangiosarcoma,| | | Metastatic, Heart | | 2 | Subcutaneous Tissue, | | | Neurofibrosarcoma | X | 1 | Subcutaneous Tissue, Sarcoma | | 2 | Subcutaneous Tissue, Sarcoma, | | | Multiple | X | 1 | Subcutaneous Tissue, Squamous Cell | | | Carcinoma, Metastatic, Stomach | X | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + | 90 | Vertebra, Lymphoma Malignant | | | Lymphocytic | | 1 | __________________________________________________________________________|____________| Skeletal Muscle | | 2 | Diaphragm, Sarcoma, Metastatic, | | | Stomach | | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + | 90 | Hemangiosarcoma, Metastatic, Heart | X | 1 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X | 10 | __________________________________________________________________________|____________| Spinal Cord | + | 9 | Lymphoma Malignant Lymphocytic | X | 8 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | A + + + + + + + + + + + + + + | 82 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + | 88 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 100 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 3| 3| 3| 2| 3| 4| 2| 3| 3| 3| 4| 4| 2| 2| 3| | | DAY ON TEST | 6| 1| 5| 5| 1| 1| 3| 7| 1| 8| 5| 1| 3| 8| 6| | | | 7| 6| 7| 7| 6| 8| 0| 6| 5| 5| 7| 8| 2| 0| 4| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | A | 625 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - cont | | | | | | Adenocarcinoma, Metastatic, Mammary | | | Gland | X | 5 | Alveolar/Bronchiolar Adenoma | | 15 | Alveolar/Bronchiolar Adenoma, | | | Multiple | X | 4 | Alveolar/Bronchiolar Carcinoma | | 5 | Alveolar/Bronchiolar Carcinoma, | | | Multiple | X | 3 | Hemangiosarcoma, Metastatic, Heart | X | 15 | Hemangiosarcoma, Metastatic, Skin | | 1 | Hepatocellular Carcinoma, Metastatic,| | | Liver | | 1 | Histiocytic Sarcoma | | 4 | Lymphoma Malignant Lymphocytic | X X X X X | 22 | Mixed Tumor Malignant, Metastatic, | | | Mammary Gland | | 3 | Sarcoma, Metastatic, Skin | X | 1 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | X | 1 | Mediastinum, Alveolar/Bronchiolar | | | Carcinoma, Metastatic, Lung | | 3 | Mediastinum, Hemangiosarcoma, | | | Metastatic, Heart | | 1 | Mediastinum, Mixed Tumor Malignant, | | | Metastatic, Mammary Gland | | 1 | Mediastinum, Sarcoma, Metastatic, | | | Skin | X | 1 | Mediastinum, Squamous Cell Carcinoma,| | | Metastatic, Stomach | X | 1 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + | 89 | Lymphoma Malignant Lymphocytic | X | 5 | __________________________________________________________________________|____________| Trachea | A + + + + + + + + + + + + + + | 83 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | + | 3 | __________________________________________________________________________|____________| Harderian Gland | + + M M + + M + + + + + M + + | 73 | Adenoma | X X X | 10 | __________________________________________________________________________|____________| Zymbal's Gland | | 2 | Adenoma | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 101 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 3| 3| 3| 2| 3| 4| 2| 3| 3| 3| 4| 4| 2| 2| 3| | | DAY ON TEST | 6| 1| 5| 5| 1| 1| 3| 7| 1| 8| 5| 1| 3| 8| 6| | | | 7| 6| 7| 7| 6| 8| 0| 6| 5| 5| 7| 8| 2| 0| 4| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | A | 625 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ SPECIAL SENSES SYSTEM - cont | | | | | | Carcinoma | | 1 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + | 90 | Alveolar/Bronchiolar Carcinoma, | | | Metastatic, Lung | | 1 | Hemangiosarcoma, Metastatic, Heart | X | 1 | Histiocytic Sarcoma | | 4 | Lymphoma Malignant Lymphocytic | X X X X X | 21 | Lymphoma Malignant | | 1 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | X | 1 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + A + + | 85 | Lymphoma Malignant Lymphocytic | X X | 9 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | X | 1 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 90 | Histiocytic Sarcoma | | 4 | Lymphoma Malignant | | 1 | Lymphoma Malignant Lymphocytic | X X X X X X X | 32 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 102 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 2| 4| 7| 2| 7| 7| 7| 6| 2| 6| 5| 6| 7| 7| 7| 2| 7| 7| 4| 7| 6| 7| 7| 7| DAY ON TEST | 3| 8| 5| 3| 8| 3| 3| 3| 2| 8| 6| 1| 5| 3| 3| 3| 8| 3| 3| 5| 3| 7| 3| 3| 3| | 6| 1| 6| 6| 1| 6| 6| 6| 7| 1| 6| 1| 2| 6| 6| 6| 1| 6| 6| 6| 6| 7| 6| 6| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| CONTROL | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + M + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + M + + + + + + + + + A + + +| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + A + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + A + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Carcinoma | X X X | Hepatocellular Carcinoma, Multiple | X X | Hepatocellular Adenoma | X X X X X | Hepatocellular Adenoma, Multiple | | Hepatocholangiocarcinoma | | Lymphoma Malignant Lymphocytic | X X | Sarcoma, Metastatic, Skin | | __________________________________________________________________________| Mesentery | + + | Fat, Sarcoma, Metastatic, Skin | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Acinus, Lymphoma Malignant | | Lymphocytic | X | Acinus, Lymphoma Malignant Mixed | X | Acinus, Sarcoma, Metastatic, Skin | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | | Sarcoma, Metastatic, Skin | | Squamous Cell Papilloma | X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Tooth | + + + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | _____________________________________________________________________________________________________________________| Page 103 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 2| 4| 7| 2| 7| 7| 7| 6| 2| 6| 5| 6| 7| 7| 7| 2| 7| 7| 4| 7| 6| 7| 7| 7| DAY ON TEST | 3| 8| 5| 3| 8| 3| 3| 3| 2| 8| 6| 1| 5| 3| 3| 3| 8| 3| 3| 5| 3| 7| 3| 3| 3| | 6| 1| 6| 6| 1| 6| 6| 6| 7| 1| 6| 1| 2| 6| 6| 6| 1| 6| 6| 6| 6| 7| 6| 6| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| CONTROL | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Lymphoma Malignant Lymphocytic | X | Bilateral, Adenoma | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | + + M + + + + + + + M M + + I + + + + + + M + + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Pars Distalis, Adenoma | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicular Cell, Adenoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Penis | | __________________________________________________________________________| Preputial Gland | + + | __________________________________________________________________________| Prostate | + + + + + + + + M + + + + + + + + + + + + M + + +| __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Interstitial Cell, Adenoma | X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Mast Cell Tumor NOS | | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangioma | | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | | Iliac, Lymphoma Malignant Mixed | X | Renal, Lymphoma Malignant Lymphocytic| X | Renal, Lymphoma Malignant Mixed | X | __________________________________________________________________________| Lymph Node, Bronchial | + + + + + + + + M + + + + + + + + + + + + + + M M| Hepatocholangiocarcinoma, Metastatic,| | Liver | | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | X X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + M + + M + + + M M M M + + + + M + + +| Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | | __________________________________________________________________________| Lymph Node, Mediastinal | + M + M M + + + + + + M M + M + M M + M M + M M M| Hepatocholangiocarcinoma, Metastatic,| | Liver | | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| Page 104 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 2| 4| 7| 2| 7| 7| 7| 6| 2| 6| 5| 6| 7| 7| 7| 2| 7| 7| 4| 7| 6| 7| 7| 7| DAY ON TEST | 3| 8| 5| 3| 8| 3| 3| 3| 2| 8| 6| 1| 5| 3| 3| 3| 8| 3| 3| 5| 3| 7| 3| 3| 3| | 6| 1| 6| 6| 1| 6| 6| 6| 7| 1| 6| 1| 2| 6| 6| 6| 1| 6| 6| 6| 6| 7| 6| 6| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| CONTROL | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | X X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant Mixed | X X | __________________________________________________________________________| Thymus | + + + + + + + M + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M + M + M M M M + M M M M M M M M M + M M I M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Sarcoma | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | | Hemangiosarcoma | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X X X X X | Alveolar/Bronchiolar Adenoma, | | Multiple | X X | Alveolar/Bronchiolar Carcinoma | X X| Alveolar/Bronchiolar Carcinoma, | | Multiple | X | Carcinoma, Metastatic, Liver | | Hepatocellular Carcinoma, Metastatic,| | Liver | X | Hepatocholangiocarcinoma, Metastatic,| | Liver | | Lymphoma Malignant Lymphocytic | X X | Sarcoma, Metastatic, Skin | | Mediastinum, | | Hepatocholangiocarcinoma, | | Metastatic, Liver | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | _____________________________________________________________________________________________________________________| Page 105 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 2| 4| 7| 2| 7| 7| 7| 6| 2| 6| 5| 6| 7| 7| 7| 2| 7| 7| 4| 7| 6| 7| 7| 7| DAY ON TEST | 3| 8| 5| 3| 8| 3| 3| 3| 2| 8| 6| 1| 5| 3| 3| 3| 8| 3| 3| 5| 3| 7| 3| 3| 3| | 6| 1| 6| 6| 1| 6| 6| 6| 7| 1| 6| 1| 2| 6| 6| 6| 1| 6| 6| 6| 6| 7| 6| 6| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| CONTROL | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM - cont | | | | | | __________________________________________________________________________| Ear | | __________________________________________________________________________| Eye | | __________________________________________________________________________| Harderian Gland | + M + + M + + + + M + + + + + + M + + + + + + + +| Adenoma | X X | __________________________________________________________________________| Zymbal's Gland | + + | Adenoma | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Urethra | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant Mixed | X X | _____________________________________________________________________________________________________________________| Page 106 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 4| 7| 4| 7| 2| 7| 2| 7| 6| 7| 4| 7| 6| 2| 7| 4| 4| 2| 7| 5| 4| 4| 5| 3| DAY ON TEST | 8| 5| 3| 5| 3| 8| 3| 8| 3| 9| 3| 5| 1| 0| 8| 3| 9| 5| 8| 3| 7| 5| 5| 4| 7| | 1| 6| 6| 6| 6| 1| 6| 1| 6| 3| 6| 6| 4| 9| 1| 6| 5| 6| 1| 6| 2| 6| 6| 3| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| CONTROL | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + A + + + + + + + A| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + A + + + + + + + A| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + A + + + + + + + A| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Carcinoma | X | Hepatocellular Carcinoma, Multiple | | Hepatocellular Adenoma | X X X| Hepatocellular Adenoma, Multiple | X | Hepatocholangiocarcinoma | X | Lymphoma Malignant Lymphocytic | | Sarcoma, Metastatic, Skin | X | __________________________________________________________________________| Mesentery | + + | Fat, Sarcoma, Metastatic, Skin | X | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Acinus, Lymphoma Malignant | | Lymphocytic | | Acinus, Lymphoma Malignant Mixed | | Acinus, Sarcoma, Metastatic, Skin | X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | Sarcoma, Metastatic, Skin | X | Squamous Cell Papilloma | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Tooth | + + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | _____________________________________________________________________________________________________________________| Page 107 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 4| 7| 4| 7| 2| 7| 2| 7| 6| 7| 4| 7| 6| 2| 7| 4| 4| 2| 7| 5| 4| 4| 5| 3| DAY ON TEST | 8| 5| 3| 5| 3| 8| 3| 8| 3| 9| 3| 5| 1| 0| 8| 3| 9| 5| 8| 3| 7| 5| 5| 4| 7| | 1| 6| 6| 6| 6| 1| 6| 1| 6| 3| 6| 6| 4| 9| 1| 6| 5| 6| 1| 6| 2| 6| 6| 3| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| CONTROL | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Lymphoma Malignant Lymphocytic | | Bilateral, Adenoma | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | + + M + + + + + + M + + + M + + M + + + + + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + I + + + + + + + + + + + + + + + + + + +| Pars Distalis, Adenoma | X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicular Cell, Adenoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Penis | + + | __________________________________________________________________________| Preputial Gland | + + + + M | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Interstitial Cell, Adenoma | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Mast Cell Tumor NOS | X | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangioma | | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | Iliac, Lymphoma Malignant Mixed | | Renal, Lymphoma Malignant Lymphocytic| | Renal, Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node, Bronchial | M + + + M + + + + + + + + + + + + + + + + + + + +| Hepatocholangiocarcinoma, Metastatic,| | Liver | X | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + M + + + + + M + + + + M + + + + + M +| Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | __________________________________________________________________________| Lymph Node, Mediastinal | I + + + + M + + + M M + M + + + M + M + + M M + +| Hepatocholangiocarcinoma, Metastatic,| | Liver | X | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| Page 108 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 4| 7| 4| 7| 2| 7| 2| 7| 6| 7| 4| 7| 6| 2| 7| 4| 4| 2| 7| 5| 4| 4| 5| 3| DAY ON TEST | 8| 5| 3| 5| 3| 8| 3| 8| 3| 9| 3| 5| 1| 0| 8| 3| 9| 5| 8| 3| 7| 5| 5| 4| 7| | 1| 6| 6| 6| 6| 1| 6| 1| 6| 3| 6| 6| 4| 9| 1| 6| 5| 6| 1| 6| 2| 6| 6| 3| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| CONTROL | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + I + + + + + + + + + + + + + + + + + + + M +| Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | | __________________________________________________________________________| Thymus | + M + + + + + + + + + + + + + I + + + + + + + + +| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Sarcoma | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | + | Hemangiosarcoma | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X X X X | Alveolar/Bronchiolar Adenoma, | | Multiple | | Alveolar/Bronchiolar Carcinoma | X | Alveolar/Bronchiolar Carcinoma, | | Multiple | | Carcinoma, Metastatic, Liver | | Hepatocellular Carcinoma, Metastatic,| | Liver | | Hepatocholangiocarcinoma, Metastatic,| | Liver | X | Lymphoma Malignant Lymphocytic | | Sarcoma, Metastatic, Skin | X | Mediastinum, | | Hepatocholangiocarcinoma, | | Metastatic, Liver | X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | _____________________________________________________________________________________________________________________| Page 109 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 4| 7| 4| 7| 2| 7| 2| 7| 6| 7| 4| 7| 6| 2| 7| 4| 4| 2| 7| 5| 4| 4| 5| 3| DAY ON TEST | 8| 5| 3| 5| 3| 8| 3| 8| 3| 9| 3| 5| 1| 0| 8| 3| 9| 5| 8| 3| 7| 5| 5| 4| 7| | 1| 6| 6| 6| 6| 1| 6| 1| 6| 3| 6| 6| 4| 9| 1| 6| 5| 6| 1| 6| 2| 6| 6| 3| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| CONTROL | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM - cont | | | | | | __________________________________________________________________________| Ear | | __________________________________________________________________________| Eye | + | __________________________________________________________________________| Harderian Gland | + + + + + M + + + + + + + + M + + + + + + + + + +| Adenoma | X X X X | __________________________________________________________________________| Zymbal's Gland | | Adenoma | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Urethra | + | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | | _____________________________________________________________________________________________________________________| Page 110 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 4| 7| 5| 7| 7| 2| 5| 7| 4| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 5| 3| 9| 3| 3| 8| 4| 3| 5| 3| 7| 3| 3| 3| 3| 3| 3| 3| 3| | | | 6| 6| 6| 6| 6| 6| 1| 0| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | CONTROL | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + M M + + + + + + + + | 64 | __________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Intestine Small | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + | 67 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + | 70 | Hepatocellular Carcinoma | X X X X | 8 | Hepatocellular Carcinoma, Multiple | X | 3 | Hepatocellular Adenoma | X X X X X X X X X | 17 | Hepatocellular Adenoma, Multiple | X | 2 | Hepatocholangiocarcinoma | | 1 | Lymphoma Malignant Lymphocytic | | 2 | Sarcoma, Metastatic, Skin | | 1 | __________________________________________________________________________|____________| Mesentery | + + | 6 | Fat, Sarcoma, Metastatic, Skin | | 1 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + | 70 | Acinus, Lymphoma Malignant | | | Lymphocytic | | 1 | Acinus, Lymphoma Malignant Mixed | | 1 | Acinus, Sarcoma, Metastatic, Skin | | 1 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + | 70 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | Sarcoma, Metastatic, Skin | | 1 | Squamous Cell Papilloma | | 1 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Tooth | + + + + | 9 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + | 70 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 111 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 4| 7| 5| 7| 7| 2| 5| 7| 4| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 5| 3| 9| 3| 3| 8| 4| 3| 5| 3| 7| 3| 3| 3| 3| 3| 3| 3| 3| | | | 6| 6| 6| 6| 6| 6| 1| 0| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | CONTROL | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ CARDIOVASCULAR SYSTEM - cont | | | | | | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + | 70 | Adenoma | | 1 | Lymphoma Malignant Lymphocytic | | 1 | Bilateral, Adenoma | X | 1 | __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + + + + + + + M M M | 58 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + | 69 | Pars Distalis, Adenoma | | 1 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + | 70 | Follicular Cell, Adenoma | X | 2 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Penis | + + | 4 | __________________________________________________________________________|____________| Preputial Gland | + + | 8 | __________________________________________________________________________|____________| Prostate | + + + + + + + + + + M + + + + + + + + + | 67 | __________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + | 70 | Interstitial Cell, Adenoma | | 1 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + | 70 | Mast Cell Tumor NOS | | 1 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + + + + + | 70 | Hemangioma | X | 1 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | Iliac, Lymphoma Malignant Mixed | | 1 | Renal, Lymphoma Malignant Lymphocytic| | 1 | Renal, Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________|____________| Lymph Node, Bronchial | + + + M + + + + + + + + + + + + + + + + | 64 | Hepatocholangiocarcinoma, Metastatic,| | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 112 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 4| 7| 5| 7| 7| 2| 5| 7| 4| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 5| 3| 9| 3| 3| 8| 4| 3| 5| 3| 7| 3| 3| 3| 3| 3| 3| 3| 3| | | | 6| 6| 6| 6| 6| 6| 1| 0| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | CONTROL | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Liver | | 1 | Lymphoma Malignant Lymphocytic | | 1 | Lymphoma Malignant Mixed | | 2 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + M + + M + M + + + + + + + M + + + | 55 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | + M M M M + M M + + M + M M + + M M + + | 37 | Hepatocholangiocarcinoma, Metastatic,| | | Liver | | 1 | Lymphoma Malignant Lymphocytic | | 1 | Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + | 68 | Lymphoma Malignant Lymphocytic | | 1 | Lymphoma Malignant Mixed | | 2 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + | 70 | Lymphoma Malignant Lymphocytic | | 2 | Lymphoma Malignant Mixed | | 2 | __________________________________________________________________________|____________| Thymus | + + + M + + + + + + + + + + + + + + + + | 66 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M + M M M M M M M M | 5 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + | 70 | Subcutaneous Tissue, Sarcoma | | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Skeletal Muscle | + | 2 | Hemangiosarcoma | X | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + | 70 | Alveolar/Bronchiolar Adenoma | X X X X X | 14 | Alveolar/Bronchiolar Adenoma, | | | Multiple | X X | 4 | Alveolar/Bronchiolar Carcinoma | X | 4 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 113 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 4| 7| 5| 7| 7| 2| 5| 7| 4| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 5| 3| 9| 3| 3| 8| 4| 3| 5| 3| 7| 3| 3| 3| 3| 3| 3| 3| 3| | | | 6| 6| 6| 6| 6| 6| 1| 0| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | CONTROL | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - cont | | | | | | Alveolar/Bronchiolar Carcinoma, | | | Multiple | X | 2 | Carcinoma, Metastatic, Liver | X | 1 | Hepatocellular Carcinoma, Metastatic,| | | Liver | X X | 3 | Hepatocholangiocarcinoma, Metastatic,| | | Liver | | 1 | Lymphoma Malignant Lymphocytic | | 2 | Sarcoma, Metastatic, Skin | | 1 | Mediastinum, | | | Hepatocholangiocarcinoma, | | | Metastatic, Liver | | 1 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Ear | + | 1 | __________________________________________________________________________|____________| Eye | | 1 | __________________________________________________________________________|____________| Harderian Gland | + + + + + + M + + + + + + + + + + + + + | 63 | Adenoma | | 6 | __________________________________________________________________________|____________| Zymbal's Gland | | 2 | Adenoma | | 1 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Urethra | + | 2 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + | 70 | Lymphoma Malignant Lymphocytic | | 2 | Lymphoma Malignant Mixed | | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 114 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 7| 4| 7| 7| 4| 7| 7| 7| 7| 7| 6| 2| 7| 7| 5| 2| 4| 7| 5| 4| 7| 7| 7| 4| DAY ON TEST | 5| 2| 5| 3| 3| 5| 3| 3| 3| 3| 3| 3| 8| 3| 3| 8| 8| 5| 3| 9| 8| 3| 3| 3| 5| | 2| 3| 6| 5| 5| 6| 5| 5| 5| 5| 5| 6| 1| 5| 5| 7| 1| 6| 5| 6| 4| 5| 5| 5| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 6.25 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Gallbladder | + + + + + + + + + A + + A + + M + + + | __________________________________________________________________________| Intestine Large | + + + + + + + + + A + + + + + A + + + | __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + A + + A + + A + + + | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + A + + + + + A + + + | __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + A + + A + + + | __________________________________________________________________________| Intestine Small | + + + + + + + + + A + + A + + + + + + | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + A + + A + + A + + + | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + A + + A + + + + + + | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + A + + A + + A + + + | Carcinoma | X | Lymphoma Malignant Mixed | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X | Hepatocellular Carcinoma | X X X X X X X | Hepatocellular Carcinoma, Multiple | X X | Hepatocellular Adenoma | X X X X X X | Hepatocellular Adenoma, Multiple | | __________________________________________________________________________| Mesentery | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + A + + + + + + | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + A + + + + + + | __________________________________________________________________________| Tooth | + + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Blood Vessel | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + | Adenoma | X X | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + A + + + + + + | Pheochromocytoma Benign | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + A + + + + + + | __________________________________________________________________________| Parathyroid Gland | + M + + + + + + + M M + M + + + M M + | __________________________________________________________________________| Pituitary Gland | + + + + + + I + + + + + + + + I + + + | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + | Follicular Cell, Adenoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| Page 115 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 7| 4| 7| 7| 4| 7| 7| 7| 7| 7| 6| 2| 7| 7| 5| 2| 4| 7| 5| 4| 7| 7| 7| 4| DAY ON TEST | 5| 2| 5| 3| 3| 5| 3| 3| 3| 3| 3| 3| 8| 3| 3| 8| 8| 5| 3| 9| 8| 3| 3| 3| 5| | 2| 3| 6| 5| 5| 6| 5| 5| 5| 5| 5| 6| 1| 5| 5| 7| 1| 6| 5| 6| 4| 5| 5| 5| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 6.25 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Penis | | __________________________________________________________________________| Preputial Gland | + | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + A + + + + + A + + + | Hemangioma | | Hemangiosarcoma, Metastatic, Spleen | X | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Lymph Node, Bronchial | I + + + + + + + + M + + + + M + + + + | __________________________________________________________________________| Lymph Node, Mandibular | M M + M + + + + + + + + + + + + + + + | __________________________________________________________________________| Lymph Node, Mediastinal | M + + + + M M + + M M M M + + + + + + | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + A + + + + M + | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + | Hemangiosarcoma | X | Lymphoma Malignant Undifferentiated | | Cell Type | | __________________________________________________________________________| Thymus | + + + + + + + I + + + + A + + + + + I | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M I M + M M M M M M M M M M M | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + | Subcutaneous Tissue, Hemangiosarcoma | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + A + + A + + + + + + | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + | Adenocarcinoma, Metastatic, | | Harderian Gland | X | Alveolar/Bronchiolar Adenoma | X X X X X | Alveolar/Bronchiolar Adenoma, | | Multiple | X X | Alveolar/Bronchiolar Carcinoma | X X X | Hepatocellular Carcinoma, Metastatic,| | _____________________________________________________________________________________________________________________| Page 116 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 7| 4| 7| 7| 4| 7| 7| 7| 7| 7| 6| 2| 7| 7| 5| 2| 4| 7| 5| 4| 7| 7| 7| 4| DAY ON TEST | 5| 2| 5| 3| 3| 5| 3| 3| 3| 3| 3| 3| 8| 3| 3| 8| 8| 5| 3| 9| 8| 3| 3| 3| 5| | 2| 3| 6| 5| 5| 6| 5| 5| 5| 5| 5| 6| 1| 5| 5| 7| 1| 6| 5| 6| 4| 5| 5| 5| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 6.25 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Liver | X X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Trachea | + + + + + + + + + + + + A + + + + + + | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X | Adenoma | X X X X X X X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + | Renal Tubule, Adenoma | X | __________________________________________________________________________| Urethra | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + A + + A + + + | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Mixed | | Lymphoma Malignant Undifferentiated | | Cell Type | | _____________________________________________________________________________________________________________________| Page 117 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 4| 7| 7| 7| 6| 7| 7| 7| 7| 7| 4| 2| 2| 4| 7| 6| 7| 7| 7| 7| 7| 7| 4| 2| DAY ON TEST | 8| 5| 3| 3| 3| 5| 3| 3| 3| 3| 3| 5| 8| 8| 5| 3| 7| 3| 3| 3| 3| 3| 3| 5| 8| | 1| 6| 5| 5| 5| 3| 5| 5| 5| 5| 5| 6| 1| 1| 6| 5| 8| 5| 5| 5| 5| 5| 5| 6| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 6.25 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + M + + + + + + + + + + + + + | __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + | Carcinoma | | Lymphoma Malignant Mixed | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + | Hemangiosarcoma | | Hepatocellular Carcinoma | X X X X X X | Hepatocellular Carcinoma, Multiple | | Hepatocellular Adenoma | X | Hepatocellular Adenoma, Multiple | X | __________________________________________________________________________| Mesentery | + + + | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Tooth | + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Blood Vessel | + | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + | Adenoma | X | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + | Pheochromocytoma Benign | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Parathyroid Gland | + + M + + M M + M + + + I + + M + | __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + | Follicular Cell, Adenoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| Page 118 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 4| 7| 7| 7| 6| 7| 7| 7| 7| 7| 4| 2| 2| 4| 7| 6| 7| 7| 7| 7| 7| 7| 4| 2| DAY ON TEST | 8| 5| 3| 3| 3| 5| 3| 3| 3| 3| 3| 5| 8| 8| 5| 3| 7| 3| 3| 3| 3| 3| 3| 5| 8| | 1| 6| 5| 5| 5| 3| 5| 5| 5| 5| 5| 6| 1| 1| 6| 5| 8| 5| 5| 5| 5| 5| 5| 6| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 6.25 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Penis | | __________________________________________________________________________| Preputial Gland | | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + | Hemangioma | X | Hemangiosarcoma, Metastatic, Spleen | | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Lymph Node, Bronchial | + + + + + + + + + M M M + + + + + | __________________________________________________________________________| Lymph Node, Mandibular | + M + + + + + I M + + + M + + + + | __________________________________________________________________________| Lymph Node, Mediastinal | + + + M + M M M + M M M + M M + + | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + | Hemangiosarcoma | | Lymphoma Malignant Undifferentiated | | Cell Type | | __________________________________________________________________________| Thymus | + + + + + + + + M + + + + M + + + | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M I M M M M M M M + M M M M | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + | Subcutaneous Tissue, Hemangiosarcoma | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + | Adenocarcinoma, Metastatic, | | Harderian Gland | | Alveolar/Bronchiolar Adenoma | X X X X X | Alveolar/Bronchiolar Adenoma, | | Multiple | X X X X | Alveolar/Bronchiolar Carcinoma | X | Hepatocellular Carcinoma, Metastatic,| | _____________________________________________________________________________________________________________________| Page 119 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 4| 7| 7| 7| 6| 7| 7| 7| 7| 7| 4| 2| 2| 4| 7| 6| 7| 7| 7| 7| 7| 7| 4| 2| DAY ON TEST | 8| 5| 3| 3| 3| 5| 3| 3| 3| 3| 3| 5| 8| 8| 5| 3| 7| 3| 3| 3| 3| 3| 3| 5| 8| | 1| 6| 5| 5| 5| 3| 5| 5| 5| 5| 5| 6| 1| 1| 6| 5| 8| 5| 5| 5| 5| 5| 5| 6| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 6.25 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Liver | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + | Adenocarcinoma | | Adenoma | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + | Renal Tubule, Adenoma | | __________________________________________________________________________| Urethra | + | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + | Lymphoma Malignant Mixed | | Lymphoma Malignant Undifferentiated | | Cell Type | | _____________________________________________________________________________________________________________________| Page 120 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 2| 7| 7| 7| 7| 2| 4| 7| 7| 4| 4| 2| 2| 7| 7| 6| 7| 7| 7| | | DAY ON TEST | 3| 8| 3| 3| 3| 1| 8| 5| 3| 3| 5| 6| 8| 8| 3| 3| 0| 3| 3| 3| | | | 5| 1| 5| 5| 5| 7| 1| 6| 5| 5| 6| 7| 1| 1| 5| 5| 1| 5| 5| 5| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | A | 6.25 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + M + + + + + | 48 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + | 47 | __________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + A + + + | 47 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + A + + + | 46 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + A + + + | 47 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + A + + + | 47 | __________________________________________________________________________|____________| Intestine Small | + + + + + + + + + + + + + + | 48 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + A + + + | 46 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + | 48 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + A + + + | 46 | Carcinoma | | 1 | Lymphoma Malignant Mixed | X | 1 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + | 61 | Hemangiosarcoma | X | 2 | Hepatocellular Carcinoma | | 13 | Hepatocellular Carcinoma, Multiple | X X | 4 | Hepatocellular Adenoma | X X X X X | 12 | Hepatocellular Adenoma, Multiple | | 1 | __________________________________________________________________________|____________| Mesentery | + + | 5 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + A + + + | 48 | __________________________________________________________________________|____________| Tooth | + | 4 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Blood Vessel | | 1 | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + M + + | 50 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + | 49 | Adenoma | X | 4 | __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + | 48 | Pheochromocytoma Benign | X | 1 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Parathyroid Gland | + + + + M + + M + + + + + + | 36 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + I + + + | 47 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 121 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 2| 7| 7| 7| 7| 2| 4| 7| 7| 4| 4| 2| 2| 7| 7| 6| 7| 7| 7| | | DAY ON TEST | 3| 8| 3| 3| 3| 1| 8| 5| 3| 3| 5| 6| 8| 8| 3| 3| 0| 3| 3| 3| | | | 5| 1| 5| 5| 5| 7| 1| 6| 5| 5| 6| 7| 1| 1| 5| 5| 1| 5| 5| 5| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | A | 6.25 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + | 50 | Follicular Cell, Adenoma | X X X | 4 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Penis | + | 1 | __________________________________________________________________________|____________| Preputial Gland | + | 2 | __________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + | 48 | Hemangioma | | 1 | Hemangiosarcoma, Metastatic, Spleen | | 1 | __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + | 50 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Blood | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Lymph Node, Bronchial | + + M + M + + + + + + + + + | 42 | __________________________________________________________________________|____________| Lymph Node, Mandibular | M M + + M + M + + + + + + + | 39 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | M M M M + + + + + + M M + M | 27 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + M + + + + + + | 47 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + | 50 | Hemangiosarcoma | | 1 | Lymphoma Malignant Undifferentiated | | | Cell Type | X | 1 | __________________________________________________________________________|____________| Thymus | + + + + M + M + + + + + + + | 43 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | + M M M M M M M M M M M M M | 3 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + | 50 | Subcutaneous Tissue, Hemangiosarcoma | | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + | 50 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 122 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 2| 7| 7| 7| 7| 2| 4| 7| 7| 4| 4| 2| 2| 7| 7| 6| 7| 7| 7| | | DAY ON TEST | 3| 8| 3| 3| 3| 1| 8| 5| 3| 3| 5| 6| 8| 8| 3| 3| 0| 3| 3| 3| | | | 5| 1| 5| 5| 5| 7| 1| 6| 5| 5| 6| 7| 1| 1| 5| 5| 1| 5| 5| 5| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | A | 6.25 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ NERVOUS SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + | 50 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + + + + + + + | 48 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + | 52 | Adenocarcinoma, Metastatic, | | | Harderian Gland | | 1 | Alveolar/Bronchiolar Adenoma | X X X X X | 15 | Alveolar/Bronchiolar Adenoma, | | | Multiple | | 6 | Alveolar/Bronchiolar Carcinoma | X X | 6 | Hepatocellular Carcinoma, Metastatic,| | | Liver | X | 3 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + | 49 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Harderian Gland | + + + + + + M + + + + + + + + + | 59 | Adenocarcinoma | | 1 | Adenoma | | 7 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + | 53 | Renal Tubule, Adenoma | | 1 | __________________________________________________________________________|____________| Urethra | + | 2 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + | 48 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + | 62 | Lymphoma Malignant Mixed | X | 1 | Lymphoma Malignant Undifferentiated | | | Cell Type | X | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 123 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 3| 7| 7| 6| 5| 7| 5| 6| 7| 6| 4| 5| 7| 6| 7| 7| 6| 2| 4| 4| 7| 7| 4| 2| 4| DAY ON TEST | 9| 3| 3| 3| 4| 3| 1| 0| 3| 8| 5| 8| 3| 1| 3| 3| 1| 8| 5| 5| 3| 3| 5| 8| 5| | 7| 4| 4| 2| 0| 4| 7| 4| 4| 0| 6| 5| 4| 8| 4| 4| 0| 1| 6| 6| 4| 4| 6| 1| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 20 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + M + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Gallbladder | A + + + + + A A + + + A + + + + + + + | __________________________________________________________________________| Intestine Large | + + + + + + + A + + + + + + + + + + + | __________________________________________________________________________| Intestine Large, Cecum | A + + + + + + A + + + + + + + + + + + | __________________________________________________________________________| Intestine Large, Colon | A + + + + + + A + + + + + + + + + + + | __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + A + + + + + + + + + + + | __________________________________________________________________________| Intestine Small | A + + + + + + A + + + + + + + + + + + | __________________________________________________________________________| Intestine Small, Duodenum | A + + + I + + A + + + A + + + + + + + | __________________________________________________________________________| Intestine Small, Ileum | A + + + + + A A + + + + + + + + + + + | __________________________________________________________________________| Intestine Small, Jejunum | A + + + + + A A + + + + + + + + + + + | Carcinoma | | Lymphoma Malignant Mixed | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Carcinoma | X X | Hepatocellular Carcinoma, Multiple | X X | Hepatocellular Adenoma | X X X | Hepatocellular Adenoma, Multiple | X X X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Mesentery | + + + + + + | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + | Acinus, Hemangiosarcoma, Metastatic, | | Heart | | Acinus, Lymphoma Malignant | | Lymphocytic | X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Tooth | + + + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | | Hepatocellular Carcinoma, Metastatic,| | Liver | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + A + + + + + + + + + + + | _____________________________________________________________________________________________________________________| Page 124 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 3| 7| 7| 6| 5| 7| 5| 6| 7| 6| 4| 5| 7| 6| 7| 7| 6| 2| 4| 4| 7| 7| 4| 2| 4| DAY ON TEST | 9| 3| 3| 3| 4| 3| 1| 0| 3| 8| 5| 8| 3| 1| 3| 3| 1| 8| 5| 5| 3| 3| 5| 8| 5| | 7| 4| 4| 2| 0| 4| 7| 4| 4| 0| 6| 5| 4| 8| 4| 4| 0| 1| 6| 6| 4| 4| 6| 1| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 20 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + | Adenoma | X | __________________________________________________________________________| Parathyroid Gland | + + + + + + + M + M M + + + + + + + + | __________________________________________________________________________| Pituitary Gland | I + + + + + + + + + + + + + + + + + + | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Penis | + M + | __________________________________________________________________________| Preputial Gland | + | __________________________________________________________________________| Prostate | + M + + M + + + + + + + + + + + + + + | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + | Axillary, Lymphoma Malignant | | Lymphocytic | X | Iliac, Histiocytic Sarcoma | | __________________________________________________________________________| Lymph Node, Bronchial | + + M + M + + + + + + + + + + + + + + | Adenocarcinoma, Metastatic, Lung | | Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Lymph Node, Mandibular | M + + + + + M M + + + + + + + + + + + | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | Sarcoma, Metastatic, Skin | X | __________________________________________________________________________| Lymph Node, Mediastinal | + + + + I M + + + + + M + M M M + M M | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + M + + | Histiocytic Sarcoma | | _____________________________________________________________________________________________________________________| Page 125 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 3| 7| 7| 6| 5| 7| 5| 6| 7| 6| 4| 5| 7| 6| 7| 7| 6| 2| 4| 4| 7| 7| 4| 2| 4| DAY ON TEST | 9| 3| 3| 3| 4| 3| 1| 0| 3| 8| 5| 8| 3| 1| 3| 3| 1| 8| 5| 5| 3| 3| 5| 8| 5| | 7| 4| 4| 2| 0| 4| 7| 4| 4| 0| 6| 5| 4| 8| 4| 4| 0| 1| 6| 6| 4| 4| 6| 1| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 20 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Thymus | + + + I + + + + M + + + + M + + + + + | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + | Subcutaneous Tissue, Hemangiosarcoma | X | Subcutaneous Tissue, Mast Cell Tumor | | NOS | | Subcutaneous Tissue, Head, Sarcoma | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Skeletal Muscle | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + | Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | | Adenocarcinoma, Multiple | | Adenocarcinoma, Metastatic, | | Harderian Gland | X | Alveolar/Bronchiolar Adenoma | X X X X X X | Alveolar/Bronchiolar Carcinoma | X X X | Alveolar/Bronchiolar Carcinoma, | | Multiple | X | Hepatocellular Carcinoma, Metastatic,| | Liver | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X | Sarcoma, Metastatic, Skin | X | _____________________________________________________________________________________________________________________| Page 126 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 3| 7| 7| 6| 5| 7| 5| 6| 7| 6| 4| 5| 7| 6| 7| 7| 6| 2| 4| 4| 7| 7| 4| 2| 4| DAY ON TEST | 9| 3| 3| 3| 4| 3| 1| 0| 3| 8| 5| 8| 3| 1| 3| 3| 1| 8| 5| 5| 3| 3| 5| 8| 5| | 7| 4| 4| 2| 0| 4| 7| 4| 4| 0| 6| 5| 4| 8| 4| 4| 0| 1| 6| 6| 4| 4| 6| 1| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 20 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Mediastinum, Sarcoma, Metastatic, | | Skin | X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X | Adenoma | X X X X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Urethra | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant Mixed | X | _____________________________________________________________________________________________________________________| Page 127 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 4| 6| 6| 2| 6| 6| 7| 6| 7| 2| 4| 2| 7| 5| 7| 5| 5| 4| 7| 6| 7| 2| 7| 6| DAY ON TEST | 3| 5| 5| 8| 8| 8| 8| 3| 5| 3| 8| 5| 8| 3| 7| 3| 8| 6| 5| 3| 8| 3| 8| 3| 3| | 4| 6| 8| 6| 1| 9| 7| 4| 1| 4| 9| 6| 1| 4| 3| 4| 6| 8| 6| 4| 2| 4| 1| 4| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 20 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + M + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + A + + + + + + M + A + + + + + A| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | I + + A + + + + + + + + A + + + + + A| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + A + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + A + + + + + A| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + A + + + + + A| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + A + + + + + A| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + A + + + + + A| Carcinoma | X | Lymphoma Malignant Mixed | | __________________________________________________________________________| Liver | + M + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Carcinoma | X X X X X X | Hepatocellular Carcinoma, Multiple | | Hepatocellular Adenoma | X X X X X X X | Hepatocellular Adenoma, Multiple | X X| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Mesentery | + | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + +| Acinus, Hemangiosarcoma, Metastatic, | | Heart | X | Acinus, Lymphoma Malignant | | Lymphocytic | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Tooth | + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X | Hepatocellular Carcinoma, Metastatic,| | Liver | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + I + + + + + +| _____________________________________________________________________________________________________________________| Page 128 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 4| 6| 6| 2| 6| 6| 7| 6| 7| 2| 4| 2| 7| 5| 7| 5| 5| 4| 7| 6| 7| 2| 7| 6| DAY ON TEST | 3| 5| 5| 8| 8| 8| 8| 3| 5| 3| 8| 5| 8| 3| 7| 3| 8| 6| 5| 3| 8| 3| 8| 3| 3| | 4| 6| 8| 6| 1| 9| 7| 4| 1| 4| 9| 6| 1| 4| 3| 4| 6| 8| 6| 4| 2| 4| 1| 4| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 20 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + +| Adenoma | | __________________________________________________________________________| Parathyroid Gland | + + + + M + + + + + + + I + + + M + +| __________________________________________________________________________| Pituitary Gland | + + + M + + + + + + + + + + + + + + I| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Penis | M +| __________________________________________________________________________| Preputial Gland | + +| __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | + | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + +| Axillary, Lymphoma Malignant | | Lymphocytic | | Iliac, Histiocytic Sarcoma | | __________________________________________________________________________| Lymph Node, Bronchial | + + + + + + M + + + + + + + + + + + M| Adenocarcinoma, Metastatic, Lung | X | Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Lymph Node, Mandibular | + + + M + M M M + + + + + + + + + M +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Sarcoma, Metastatic, Skin | | __________________________________________________________________________| Lymph Node, Mediastinal | M + + + + M + + + M + M + + M M + M +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Lymph Node, Mesenteric | I + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | _____________________________________________________________________________________________________________________| Page 129 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 4| 6| 6| 2| 6| 6| 7| 6| 7| 2| 4| 2| 7| 5| 7| 5| 5| 4| 7| 6| 7| 2| 7| 6| DAY ON TEST | 3| 5| 5| 8| 8| 8| 8| 3| 5| 3| 8| 5| 8| 3| 7| 3| 8| 6| 5| 3| 8| 3| 8| 3| 3| | 4| 6| 8| 6| 1| 9| 7| 4| 1| 4| 9| 6| 1| 4| 3| 4| 6| 8| 6| 4| 2| 4| 1| 4| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 20 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Thymus | + + + + M + M + + + M + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | I M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Hemangiosarcoma | | Subcutaneous Tissue, Mast Cell Tumor | | NOS | X | Subcutaneous Tissue, Head, Sarcoma | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | M | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X | Adenocarcinoma, Multiple | X | Adenocarcinoma, Metastatic, | | Harderian Gland | | Alveolar/Bronchiolar Adenoma | X X | Alveolar/Bronchiolar Carcinoma | X X X | Alveolar/Bronchiolar Carcinoma, | | Multiple | X | Hepatocellular Carcinoma, Metastatic,| | Liver | X X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | | Sarcoma, Metastatic, Skin | | _____________________________________________________________________________________________________________________| Page 130 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 7| 4| 6| 6| 2| 6| 6| 7| 6| 7| 2| 4| 2| 7| 5| 7| 5| 5| 4| 7| 6| 7| 2| 7| 6| DAY ON TEST | 3| 5| 5| 8| 8| 8| 8| 3| 5| 3| 8| 5| 8| 3| 7| 3| 8| 6| 5| 3| 8| 3| 8| 3| 3| | 4| 6| 8| 6| 1| 9| 7| 4| 1| 4| 9| 6| 1| 4| 3| 4| 6| 8| 6| 4| 2| 4| 1| 4| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 20 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Mediastinum, Sarcoma, Metastatic, | | Skin | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | | Adenoma | X X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Urethra | + | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | | _____________________________________________________________________________________________________________________| Page 131 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 2| 4| 2| 2| 7| 7| 6| 2| 7| 7| 7| 5| 2| 5| 4| 5| 4| 7| 6| 7| | | DAY ON TEST | 8| 5| 8| 8| 3| 3| 5| 8| 3| 3| 3| 3| 8| 3| 5| 3| 5| 3| 9| 3| | | | 1| 6| 1| 1| 4| 4| 9| 1| 4| 4| 4| 1| 1| 2| 6| 6| 6| 4| 3| 4| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | A | 20 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + M + + + + + + + | 47 | __________________________________________________________________________|____________| Gallbladder | + + A + + I + + A + + + | 39 | __________________________________________________________________________|____________| Intestine Large | + + A + + + + + A + + + | 47 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + A + + + + + A + + + | 42 | __________________________________________________________________________|____________| Intestine Large, Colon | + + A + + + + + A + + + | 45 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + A + + + + + A + + + | 46 | __________________________________________________________________________|____________| Intestine Small | + + A + + + + + A + + + | 44 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + A + + + + + A + + + | 42 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + A + + + + + A + + + | 43 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + A + + + + + A + + + | 43 | Carcinoma | | 1 | Lymphoma Malignant Mixed | X | 1 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + | 61 | Hepatocellular Carcinoma | X X | 10 | Hepatocellular Carcinoma, Multiple | X X X X | 6 | Hepatocellular Adenoma | X X X X X X | 16 | Hepatocellular Adenoma, Multiple | X X X | 8 | Histiocytic Sarcoma | X X | 4 | Lymphoma Malignant Lymphocytic | | 2 | __________________________________________________________________________|____________| Mesentery | + + | 9 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + A + + + | 49 | Acinus, Hemangiosarcoma, Metastatic, | | | Heart | | 1 | Acinus, Lymphoma Malignant | | | Lymphocytic | | 1 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + | 50 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + | 60 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + | 60 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + | 60 | __________________________________________________________________________|____________| Tooth | + + | 6 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 60 | Hemangiosarcoma | | 1 | Hepatocellular Carcinoma, Metastatic,| | | Liver | X | 1 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 132 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 2| 4| 2| 2| 7| 7| 6| 2| 7| 7| 7| 5| 2| 5| 4| 5| 4| 7| 6| 7| | | DAY ON TEST | 8| 5| 8| 8| 3| 3| 5| 8| 3| 3| 3| 3| 8| 3| 5| 3| 5| 3| 9| 3| | | | 1| 6| 1| 1| 4| 4| 9| 1| 4| 4| 4| 1| 1| 2| 6| 6| 6| 4| 3| 4| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | A | 20 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ CARDIOVASCULAR SYSTEM - cont | | | | | | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + M + | 47 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + A + + + | 49 | Adenoma | | 1 | __________________________________________________________________________|____________| Parathyroid Gland | + + M + + + + M + + + + | 42 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + | 47 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + | 50 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + | 50 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Penis | M M + | 4 | __________________________________________________________________________|____________| Preputial Gland | + | 4 | __________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + | 48 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Seminal Vesicle | + + A + + + + + A + + + | 47 | __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + | 51 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Blood | | 1 | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + | 50 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | | 2 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + | 50 | Axillary, Lymphoma Malignant | | | Lymphocytic | | 1 | Iliac, Histiocytic Sarcoma | X | 1 | __________________________________________________________________________|____________| Lymph Node, Bronchial | + + + + + + M M M + + M | 42 | Adenocarcinoma, Metastatic, Lung | | 1 | Alveolar/Bronchiolar Carcinoma, | | | Metastatic, Lung | | 1 | Histiocytic Sarcoma | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 133 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 2| 4| 2| 2| 7| 7| 6| 2| 7| 7| 7| 5| 2| 5| 4| 5| 4| 7| 6| 7| | | DAY ON TEST | 8| 5| 8| 8| 3| 3| 5| 8| 3| 3| 3| 3| 8| 3| 5| 3| 5| 3| 9| 3| | | | 1| 6| 1| 1| 4| 4| 9| 1| 4| 4| 4| 1| 1| 2| 6| 6| 6| 4| 3| 4| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | A | 20 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Lymphoma Malignant Lymphocytic | | 2 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + M M + + + M + M | 38 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant Lymphocytic | | 1 | Sarcoma, Metastatic, Skin | | 1 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | M M + + + + + + + + + + | 33 | Histiocytic Sarcoma | | 2 | Lymphoma Malignant Lymphocytic | | 2 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + M + + + + + + + + | 47 | Histiocytic Sarcoma | X X | 3 | Lymphoma Malignant Lymphocytic | | 1 | Lymphoma Malignant Mixed | X | 2 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + | 51 | Hemangiosarcoma | | 1 | Histiocytic Sarcoma | X X | 4 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Thymus | + + + + + M M + + + + + | 42 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | | 2 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M M M M I M M M M M M M | | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + | 51 | Subcutaneous Tissue, Hemangiosarcoma | | 1 | Subcutaneous Tissue, Mast Cell Tumor | | | NOS | | 1 | Subcutaneous Tissue, Head, Sarcoma | | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Skeletal Muscle | | | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + | 50 | Hemangiosarcoma, Metastatic, Heart | | 1 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 134 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 2| 4| 2| 2| 7| 7| 6| 2| 7| 7| 7| 5| 2| 5| 4| 5| 4| 7| 6| 7| | | DAY ON TEST | 8| 5| 8| 8| 3| 3| 5| 8| 3| 3| 3| 3| 8| 3| 5| 3| 5| 3| 9| 3| | | | 1| 6| 1| 1| 4| 4| 9| 1| 4| 4| 4| 1| 1| 2| 6| 6| 6| 4| 3| 4| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | A | 20 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + | 62 | Adenocarcinoma | | 1 | Adenocarcinoma, Multiple | | 1 | Adenocarcinoma, Metastatic, | | | Harderian Gland | | 1 | Alveolar/Bronchiolar Adenoma | X X X | 11 | Alveolar/Bronchiolar Carcinoma | X | 7 | Alveolar/Bronchiolar Carcinoma, | | | Multiple | | 2 | Hepatocellular Carcinoma, Metastatic,| | | Liver | X X | 4 | Histiocytic Sarcoma | X X | 4 | Lymphoma Malignant Lymphocytic | | 2 | Sarcoma, Metastatic, Skin | | 1 | Mediastinum, Sarcoma, Metastatic, | | | Skin | | 1 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + | 50 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + | 50 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | | 1 | __________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + | 60 | Adenocarcinoma | | 1 | Adenoma | X X X X | 10 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + | 54 | Hemangiosarcoma, Metastatic, Heart | | 1 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Urethra | | 1 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + | 50 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + | 64 | Histiocytic Sarcoma | X X | 4 | Lymphoma Malignant Lymphocytic | | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 135 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 2| 4| 2| 2| 7| 7| 6| 2| 7| 7| 7| 5| 2| 5| 4| 5| 4| 7| 6| 7| | | DAY ON TEST | 8| 5| 8| 8| 3| 3| 5| 8| 3| 3| 3| 3| 8| 3| 5| 3| 5| 3| 9| 3| | | | 1| 6| 1| 1| 4| 4| 9| 1| 4| 4| 4| 1| 1| 2| 6| 6| 6| 4| 3| 4| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | A | 20 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS - cont | | | | | | Lymphoma Malignant Mixed | X | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 136 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 7| 7| 3| 7| 2| 6| 4| 7| 4| 5| 7| 6| 7| 4| 2| 5| 2| 4| 6| 5| 7| 6| 7| 7| DAY ON TEST | 5| 3| 3| 9| 3| 8| 3| 8| 3| 5| 7| 3| 9| 3| 5| 8| 4| 8| 5| 0| 2| 3| 9| 3| 1| | 6| 3| 3| 8| 3| 1| 5| 1| 3| 6| 4| 3| 6| 3| 6| 1| 6| 1| 6| 7| 5| 3| 5| 3| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 62.5 PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + M + M + + + + + + +| __________________________________________________________________________| Gallbladder | + + A + A M + + + + + M A + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + I + A + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + A + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + A + + + + + + + + + + + +| Histiocytic Sarcoma | X | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Histiocytic | | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + A + + + + + + + + + + + +| Carcinoma | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + +| Hemangioma | | Hemangiosarcoma, Metastatic, Heart | X | Hepatocellular Carcinoma | X X X | Hepatocellular Carcinoma, Multiple | X | Hepatocellular Adenoma | X X X X X | Hepatocellular Adenoma, Multiple | X X X| Histiocytic Sarcoma | X X X X | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Mesentery | + + + + + | Fat, Hemangioma | | Fat, Hemangiosarcoma, Metastatic, | | Heart | | Fat, Histiocytic Sarcoma | X | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + +| Acinus, Lymphoma Malignant | | Lymphocytic | | __________________________________________________________________________| Pharynx | + | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + +| Squamous Cell Papilloma | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Tooth | + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Lung | | Hemangiosarcoma | X | _____________________________________________________________________________________________________________________| Page 137 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 7| 7| 3| 7| 2| 6| 4| 7| 4| 5| 7| 6| 7| 4| 2| 5| 2| 4| 6| 5| 7| 6| 7| 7| DAY ON TEST | 5| 3| 3| 9| 3| 8| 3| 8| 3| 5| 7| 3| 9| 3| 5| 8| 4| 8| 5| 0| 2| 3| 9| 3| 1| | 6| 3| 3| 8| 3| 1| 5| 1| 3| 6| 4| 3| 6| 3| 6| 1| 6| 1| 6| 7| 5| 3| 5| 3| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 62.5 PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + +| Adenoma | | Histiocytic Sarcoma | X X | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + M + + + + + + + + + I + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | + M + + + + + + + + + + M M + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + +| Pars Distalis, Carcinoma | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + +| Follicular Cell, Adenoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Penis | + + | __________________________________________________________________________| Preputial Gland | + + + | Hemangiosarcoma | X | __________________________________________________________________________| Prostate | + M + + + + + + + + + + + + + + + +| __________________________________________________________________________| Seminal Vesicle | + + + + + A + + + + + + + + + + + +| __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + +| Hemangioma | X | Interstitial Cell, Adenoma | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + +| Iliac, Histiocytic Sarcoma | X | Iliac, Lymphoma Malignant Lymphocytic| | Pancreatic, Histiocytic Sarcoma | X | Renal, Lymphoma Malignant Lymphocytic| | __________________________________________________________________________| Lymph Node, Bronchial | + + M + + M + + + M + M M I + + + + + + +| Adenocarcinoma, Metastatic, Lung | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Lymph Node, Mandibular | M + M + M + + M + + + + + + + + + M + M M| _____________________________________________________________________________________________________________________| Page 138 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 7| 7| 3| 7| 2| 6| 4| 7| 4| 5| 7| 6| 7| 4| 2| 5| 2| 4| 6| 5| 7| 6| 7| 7| DAY ON TEST | 5| 3| 3| 9| 3| 8| 3| 8| 3| 5| 7| 3| 9| 3| 5| 8| 4| 8| 5| 0| 2| 3| 9| 3| 1| | 6| 3| 3| 8| 3| 1| 5| 1| 3| 6| 4| 3| 6| 3| 6| 1| 6| 1| 6| 7| 5| 3| 5| 3| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 62.5 PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Lymph Node, Mediastinal | M + + + M M M + + M + + + + + + + M + + M| Adenocarcinoma, Metastatic, Lung | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X X | Lymphoma Malignant Histiocytic | | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X X | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Thymus | + M + + + M M + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + +| Sebaceous Gland, Adenoma | | Subcutaneous Tissue, Leiomyosarcoma | X| Subcutaneous Tissue, Mixed Tumor | | Malignant | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | | Hemangiosarcoma | | Diaphragm, Adenocarcinoma, | | Metastatic, Lung | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Pituitary | | Gland | | Histiocytic Sarcoma | X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | | Adenocarcinoma, Metastatic, | | Harderian Gland | X| Alveolar/Bronchiolar Adenoma | X X X X X X X X | _____________________________________________________________________________________________________________________| Page 139 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 7| 7| 3| 7| 2| 6| 4| 7| 4| 5| 7| 6| 7| 4| 2| 5| 2| 4| 6| 5| 7| 6| 7| 7| DAY ON TEST | 5| 3| 3| 9| 3| 8| 3| 8| 3| 5| 7| 3| 9| 3| 5| 8| 4| 8| 5| 0| 2| 3| 9| 3| 1| | 6| 3| 3| 8| 3| 1| 5| 1| 3| 6| 4| 3| 6| 3| 6| 1| 6| 1| 6| 7| 5| 3| 5| 3| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 62.5 PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Alveolar/Bronchiolar Adenoma, | | Multiple | X X X X| Alveolar/Bronchiolar Carcinoma | X X X | Alveolar/Bronchiolar Carcinoma, | | Multiple | | Hemangiosarcoma, Metastatic, Heart | X | Hemangiosarcoma, Metastatic, | | Skeletal Muscle | | Hepatocellular Carcinoma, Metastatic,| | Liver | | Histiocytic Sarcoma | X X X X | Leiomyosarcoma, Metastatic, Skin | X| Lymphoma Malignant Lymphocytic | | Lymphoma Malignant | | Mediastinum, Adenocarcinoma, | | Metastatic, Lung | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + +| Leiomyosarcoma, Metastatic, Skin | X| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | + | __________________________________________________________________________| Eye | M + + M | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X| Adenoma | X X X X X X X X | Leiomyosarcoma, Metastatic, Skin | X| Bilateral, Adenoma | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Lung | | Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | X X X | Renal Tubule, Adenocarcinoma | | Renal Tubule, Adenoma | X | __________________________________________________________________________| Urethra | + + | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X X X | Lymphoma Malignant | | Lymphoma Malignant Histiocytic | | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| Page 140 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 3| 7| 2| 4| 4| 4| 5| 7| 2| 5| 7| 2| 4| 7| 2| 2| 5| 6| 6| 5| 7| 7| 6| 2| 5| DAY ON TEST | 5| 3| 8| 5| 6| 5| 4| 3| 0| 9| 3| 8| 5| 1| 8| 8| 9| 5| 4| 3| 3| 3| 6| 8| 4| | 2| 3| 1| 6| 4| 6| 6| 3| 0| 5| 3| 1| 6| 6| 1| 1| 6| 8| 9| 7| 3| 3| 2| 1| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 62.5 PPM | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + A + + + + + A A M I + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Intestine Large | + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + A + + + + + + A + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + A + + + + + + A + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + A + + + + + + A + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + A + + + + + M A + + + +| Histiocytic Sarcoma | | __________________________________________________________________________| Intestine Small, Ileum | + + + + + A + + + + + + A + + + +| Lymphoma Malignant Histiocytic | | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + A + + + + + + A + + + +| Carcinoma | X | __________________________________________________________________________| Liver | + + + + + + + A + + + + + + + + + + + +| Hemangioma | | Hemangiosarcoma, Metastatic, Heart | X | Hepatocellular Carcinoma | X X | Hepatocellular Carcinoma, Multiple | X X X X | Hepatocellular Adenoma | X X X X| Hepatocellular Adenoma, Multiple | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Mesentery | + + + | Fat, Hemangioma | | Fat, Hemangiosarcoma, Metastatic, | | Heart | | Fat, Histiocytic Sarcoma | | __________________________________________________________________________| Pancreas | + + + + + + + A + + + + + + + + + + + +| Acinus, Lymphoma Malignant | | Lymphocytic | X | __________________________________________________________________________| Pharynx | | __________________________________________________________________________| Salivary Glands | + + + + + A + + + + + + + + + + +| Histiocytic Sarcoma | | __________________________________________________________________________| Stomach | + + + + + + + A + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + A + + + + + + + + + + + + +| Squamous Cell Papilloma | X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + A + + + + + + + + + + + + +| __________________________________________________________________________| Tooth | + + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + A + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Lung | X| Hemangiosarcoma | X X | _____________________________________________________________________________________________________________________| Page 141 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 3| 7| 2| 4| 4| 4| 5| 7| 2| 5| 7| 2| 4| 7| 2| 2| 5| 6| 6| 5| 7| 7| 6| 2| 5| DAY ON TEST | 5| 3| 8| 5| 6| 5| 4| 3| 0| 9| 3| 8| 5| 1| 8| 8| 9| 5| 4| 3| 3| 3| 6| 8| 4| | 2| 3| 1| 6| 4| 6| 6| 3| 0| 5| 3| 1| 6| 6| 1| 1| 6| 8| 9| 7| 3| 3| 2| 1| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 62.5 PPM | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + M + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + M + + + + + + + + + + +| Adenoma | | Histiocytic Sarcoma | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + M + + + + + + A + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + A + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | + + + + + M M M + M + + + + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + A + + + + + + + + + + +| Pars Distalis, Carcinoma | X | __________________________________________________________________________| Thyroid Gland | + + + + + A + + + + + + + + + + +| Follicular Cell, Adenoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Penis | + + + | __________________________________________________________________________| Preputial Gland | + + + + | Hemangiosarcoma | | __________________________________________________________________________| Prostate | + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Seminal Vesicle | + + + + + A + + + + + + A + + + +| __________________________________________________________________________| Testes | + + + + + A + + + + + + + + + + +| Hemangioma | | Interstitial Cell, Adenoma | X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + A + + + + + + + + + + + +| Histiocytic Sarcoma | | __________________________________________________________________________| Lymph Node | + + + + + + + A + + + + + + + + + + + +| Iliac, Histiocytic Sarcoma | | Iliac, Lymphoma Malignant Lymphocytic| | Pancreatic, Histiocytic Sarcoma | | Renal, Lymphoma Malignant Lymphocytic| | __________________________________________________________________________| Lymph Node, Bronchial | + + M M M M + A + M M + + + + + + + + +| Adenocarcinoma, Metastatic, Lung | X| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Lymph Node, Mandibular | M + I M M + M A M M M M + + + + + + + M| _____________________________________________________________________________________________________________________| Page 142 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 3| 7| 2| 4| 4| 4| 5| 7| 2| 5| 7| 2| 4| 7| 2| 2| 5| 6| 6| 5| 7| 7| 6| 2| 5| DAY ON TEST | 5| 3| 8| 5| 6| 5| 4| 3| 0| 9| 3| 8| 5| 1| 8| 8| 9| 5| 4| 3| 3| 3| 6| 8| 4| | 2| 3| 1| 6| 4| 6| 6| 3| 0| 5| 3| 1| 6| 6| 1| 1| 6| 8| 9| 7| 3| 3| 2| 1| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 62.5 PPM | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Lymph Node, Mediastinal | M M + + I + M A M M M + + M + M M + + +| Adenocarcinoma, Metastatic, Lung | X| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + M + + + A + + + + + + A + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Histiocytic | | Lymphoma Malignant Lymphocytic | X X X | __________________________________________________________________________| Spleen | + + + + + + + A + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Thymus | + + + + + + + A M + + + + + + + A + + I +| Lymphoma Malignant Lymphocytic | X X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M + M M M M M M M M| __________________________________________________________________________| Skin | + + + + + A + + + + + + + + + + + +| Sebaceous Gland, Adenoma | X | Subcutaneous Tissue, Leiomyosarcoma | | Subcutaneous Tissue, Mixed Tumor | | Malignant | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + A + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | +| Hemangiosarcoma | | Diaphragm, Adenocarcinoma, | | Metastatic, Lung | X| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + A + + + + + + + + + + +| Carcinoma, Metastatic, Pituitary | | Gland | X | Histiocytic Sarcoma | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + A + + + + + A A + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X| Adenocarcinoma, Metastatic, | | Harderian Gland | X | Alveolar/Bronchiolar Adenoma | X X X X| _____________________________________________________________________________________________________________________| Page 143 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 3| 7| 2| 4| 4| 4| 5| 7| 2| 5| 7| 2| 4| 7| 2| 2| 5| 6| 6| 5| 7| 7| 6| 2| 5| DAY ON TEST | 5| 3| 8| 5| 6| 5| 4| 3| 0| 9| 3| 8| 5| 1| 8| 8| 9| 5| 4| 3| 3| 3| 6| 8| 4| | 2| 3| 1| 6| 4| 6| 6| 3| 0| 5| 3| 1| 6| 6| 1| 1| 6| 8| 9| 7| 3| 3| 2| 1| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 62.5 PPM | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Alveolar/Bronchiolar Adenoma, | | Multiple | | Alveolar/Bronchiolar Carcinoma | X X X | Alveolar/Bronchiolar Carcinoma, | | Multiple | X | Hemangiosarcoma, Metastatic, Heart | X | Hemangiosarcoma, Metastatic, | | Skeletal Muscle | | Hepatocellular Carcinoma, Metastatic,| | Liver | X X | Histiocytic Sarcoma | | Leiomyosarcoma, Metastatic, Skin | | Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant | X | Mediastinum, Adenocarcinoma, | | Metastatic, Lung | X| __________________________________________________________________________| Nose | + + + + + A + + + + + A + + + + +| Leiomyosarcoma, Metastatic, Skin | | __________________________________________________________________________| Trachea | + + + + + A + + + + + A A + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | | __________________________________________________________________________| Eye | +| __________________________________________________________________________| Harderian Gland | + + + + + + + M + + + + + + + + + + + +| Adenocarcinoma | X | Adenoma | X X X X X X X | Leiomyosarcoma, Metastatic, Skin | | Bilateral, Adenoma | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + A + + + + + + + + + + +| Adenocarcinoma, Metastatic, Lung | X| Hemangiosarcoma, Metastatic, Heart | X | Histiocytic Sarcoma | | Renal Tubule, Adenocarcinoma | X | Renal Tubule, Adenoma | | __________________________________________________________________________| Urethra | + | __________________________________________________________________________| Urinary Bladder | + + + + + A + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant | X | Lymphoma Malignant Histiocytic | | Lymphoma Malignant Lymphocytic | X X X | _____________________________________________________________________________________________________________________| Page 144 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 6| 4| 7| 7| 4| 7| 6| 7| 0| 2| 2| 4| 4| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 2| 5| 3| 3| 5| 3| 8| 3| 1| 8| 8| 5| 3| 3| 0| 3| 3| 3| 0| | | | 3| 4| 6| 3| 3| 6| 3| 0| 3| 2| 1| 1| 6| 4| 3| 7| 3| 3| 3| 7| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| | A | 62.5 PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + M + + + + + I + + + + | 44 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + A M A + + + + | 36 | Lymphoma Malignant Lymphocytic | X | 1 | __________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + + + + + | 48 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + A + + + + | 44 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + | 46 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + | 48 | __________________________________________________________________________|____________| Intestine Small | + + + + + + + + + + + + + + | 47 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + A + + + + | 44 | Histiocytic Sarcoma | | 1 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + I + + + + + + | 46 | Lymphoma Malignant Histiocytic | X | 1 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + A + A + + + + | 44 | Carcinoma | | 1 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + | 60 | Hemangioma | X | 1 | Hemangiosarcoma, Metastatic, Heart | X | 3 | Hepatocellular Carcinoma | X X X X X X | 11 | Hepatocellular Carcinoma, Multiple | X | 6 | Hepatocellular Adenoma | X X X X X | 14 | Hepatocellular Adenoma, Multiple | X X | 5 | Histiocytic Sarcoma | X | 5 | Lymphoma Malignant Lymphocytic | X | 2 | __________________________________________________________________________|____________| Mesentery | + + + + + + | 14 | Fat, Hemangioma | X X | 2 | Fat, Hemangiosarcoma, Metastatic, | | | Heart | X | 1 | Fat, Histiocytic Sarcoma | | 1 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + | 58 | Acinus, Lymphoma Malignant | | | Lymphocytic | X | 2 | __________________________________________________________________________|____________| Pharynx | | 1 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + | 48 | Histiocytic Sarcoma | | 1 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + + + + | 60 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + | 60 | Squamous Cell Papilloma | | 1 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + | 60 | __________________________________________________________________________|____________| Tooth | | 3 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 145 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 6| 4| 7| 7| 4| 7| 6| 7| 0| 2| 2| 4| 4| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 2| 5| 3| 3| 5| 3| 8| 3| 1| 8| 8| 5| 3| 3| 0| 3| 3| 3| 0| | | | 3| 4| 6| 3| 3| 6| 3| 0| 3| 2| 1| 1| 6| 4| 3| 7| 3| 3| 3| 7| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| | A | 62.5 PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM - cont | | | | | | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + | 58 | Adenocarcinoma, Metastatic, Lung | X | 2 | Hemangiosarcoma | X X | 5 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | | 1 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + M + + + + + + + + + + + + | 47 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + M + + + + + + + + + + + + | 47 | Adenoma | X | 1 | Histiocytic Sarcoma | | 2 | __________________________________________________________________________|____________| Adrenal Gland, Medulla | + M + + + + + + + + + + + + | 44 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + | 58 | __________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + M M + + + + | 40 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + | 48 | Pars Distalis, Carcinoma | | 1 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + | 48 | Follicular Cell, Adenoma | X X | 4 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + | 48 | __________________________________________________________________________|____________| Penis | + + | 7 | __________________________________________________________________________|____________| Preputial Gland | + + | 9 | Hemangiosarcoma | | 1 | __________________________________________________________________________|____________| Prostate | + + + + + + + + + + + I M + | 45 | __________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + | 46 | __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + | 48 | Hemangioma | | 1 | Interstitial Cell, Adenoma | | 1 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Blood | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + | 58 | Histiocytic Sarcoma | | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 146 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 6| 4| 7| 7| 4| 7| 6| 7| 0| 2| 2| 4| 4| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 2| 5| 3| 3| 5| 3| 8| 3| 1| 8| 8| 5| 3| 3| 0| 3| 3| 3| 0| | | | 3| 4| 6| 3| 3| 6| 3| 0| 3| 2| 1| 1| 6| 4| 3| 7| 3| 3| 3| 7| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| | A | 62.5 PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + + | 57 | Iliac, Histiocytic Sarcoma | | 1 | Iliac, Lymphoma Malignant Lymphocytic| X | 1 | Pancreatic, Histiocytic Sarcoma | | 1 | Renal, Lymphoma Malignant Lymphocytic| X | 1 | __________________________________________________________________________|____________| Lymph Node, Bronchial | + + M M + M M + + + + + + + + + M | 40 | Adenocarcinoma, Metastatic, Lung | X | 2 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X | 2 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + I M + + + + M + + + + + | 37 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant Lymphocytic | X | 2 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | + + + + + + + + + + M + + + + + + | 39 | Adenocarcinoma, Metastatic, Lung | X | 2 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant Lymphocytic | X | 3 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + | 55 | Histiocytic Sarcoma | X | 4 | Lymphoma Malignant Histiocytic | X | 1 | Lymphoma Malignant Lymphocytic | X | 4 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + | 58 | Histiocytic Sarcoma | X | 4 | Lymphoma Malignant Lymphocytic | X | 2 | __________________________________________________________________________|____________| Thymus | + + + + + + M M + + + + + + + + + | 51 | Lymphoma Malignant Lymphocytic | X | 3 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M | 1 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + | 49 | Sebaceous Gland, Adenoma | | 1 | Subcutaneous Tissue, Leiomyosarcoma | | 1 | Subcutaneous Tissue, Mixed Tumor | | | Malignant | | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Skeletal Muscle | + | 2 | Hemangiosarcoma | X | 1 | Diaphragm, Adenocarcinoma, | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 147 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 6| 4| 7| 7| 4| 7| 6| 7| 0| 2| 2| 4| 4| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 2| 5| 3| 3| 5| 3| 8| 3| 1| 8| 8| 5| 3| 3| 0| 3| 3| 3| 0| | | | 3| 4| 6| 3| 3| 6| 3| 0| 3| 2| 1| 1| 6| 4| 3| 7| 3| 3| 3| 7| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| | A | 62.5 PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ MUSCULOSKELETAL SYSTEM - cont | | | | | | Metastatic, Lung | | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + | 48 | Carcinoma, Metastatic, Pituitary | | | Gland | | 1 | Histiocytic Sarcoma | | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + + + + + + + | 46 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + | 69 | Adenocarcinoma | X | 2 | Adenocarcinoma, Metastatic, | | | Harderian Gland | | 2 | Alveolar/Bronchiolar Adenoma | X X X X X X X X | 20 | Alveolar/Bronchiolar Adenoma, | | | Multiple | X X | 6 | Alveolar/Bronchiolar Carcinoma | X X X | 9 | Alveolar/Bronchiolar Carcinoma, | | | Multiple | X | 2 | Hemangiosarcoma, Metastatic, Heart | X | 3 | Hemangiosarcoma, Metastatic, | | | Skeletal Muscle | X | 1 | Hepatocellular Carcinoma, Metastatic,| | | Liver | X | 3 | Histiocytic Sarcoma | | 4 | Leiomyosarcoma, Metastatic, Skin | | 1 | Lymphoma Malignant Lymphocytic | X | 2 | Lymphoma Malignant | | 1 | Mediastinum, Adenocarcinoma, | | | Metastatic, Lung | X | 2 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + | 47 | Leiomyosarcoma, Metastatic, Skin | | 1 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + | 46 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Ear | | 1 | __________________________________________________________________________|____________| Eye | | 3 | __________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + M + + + + + + | 57 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 148 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 7| 6| 4| 7| 7| 4| 7| 6| 7| 0| 2| 2| 4| 4| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 2| 5| 3| 3| 5| 3| 8| 3| 1| 8| 8| 5| 3| 3| 0| 3| 3| 3| 0| | | | 3| 4| 6| 3| 3| 6| 3| 0| 3| 2| 1| 1| 6| 4| 3| 7| 3| 3| 3| 7| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| | A | 62.5 PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ SPECIAL SENSES SYSTEM - cont | | | | | | Adenocarcinoma | X | 3 | Adenoma | X X X X X X | 21 | Leiomyosarcoma, Metastatic, Skin | | 1 | Bilateral, Adenoma | X | 2 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + | 50 | Adenocarcinoma, Metastatic, Lung | X | 2 | Hemangiosarcoma, Metastatic, Heart | | 1 | Histiocytic Sarcoma | | 3 | Renal Tubule, Adenocarcinoma | | 1 | Renal Tubule, Adenoma | X | 2 | __________________________________________________________________________|____________| Urethra | + + + | 6 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + | 49 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + | 69 | Histiocytic Sarcoma | X | 5 | Lymphoma Malignant | | 1 | Lymphoma Malignant Histiocytic | X | 1 | Lymphoma Malignant Lymphocytic | X | 4 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 149 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 6| 3| 5| 5| 5| 4| 6| 6| 2| 1| 2| 2| 5| 6| 4| 5| 2| 6| 6| 4| 7| 6| 5| 6| DAY ON TEST | 8| 7| 8| 6| 9| 9| 9| 7| 4| 8| 7| 1| 8| 9| 6| 5| 3| 8| 9| 2| 5| 3| 2| 9| 2| | 1| 4| 2| 8| 6| 6| 5| 3| 9| 1| 1| 7| 1| 6| 0| 6| 7| 1| 7| 9| 6| 3| 4| 8| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 200 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + A M + + A A + A + + + + A| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + A + + + + A + A + + + A A| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Intestine Small | + + + + + + + + + + A + + + + A + A + + + + A| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + A + + + A M + A + + + + A| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + A + + + + A + A + + + A A| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + A A + + + A + A + + + A A| Carcinoma | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + A| Adenocarcinoma, Metastatic, Lung | X | Hemangiosarcoma, Metastatic, Heart | X X X | Hepatocellular Carcinoma | X X X X X X | Hepatocellular Carcinoma, Multiple | X X X X X | Hepatocellular Adenoma | X X X X X | Hepatocellular Adenoma, Multiple | | Hepatocholangiocarcinoma | X | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | | __________________________________________________________________________| Mesentery | + + + + + + | Fat, Adenocarcinoma, Metastatic, Lung| X | Fat, Hemangiosarcoma, Metastatic, | | Spleen | | Fat, Histiocytic Sarcoma | X | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + A| Acinus, Histiocytic Sarcoma | | Duct, Adenoma, Multiple | X | __________________________________________________________________________| Pharynx | + | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | | Squamous Cell Carcinoma | X| Squamous Cell Papilloma | X X X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Tooth | + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | _____________________________________________________________________________________________________________________| Page 150 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 6| 3| 5| 5| 5| 4| 6| 6| 2| 1| 2| 2| 5| 6| 4| 5| 2| 6| 6| 4| 7| 6| 5| 6| DAY ON TEST | 8| 7| 8| 6| 9| 9| 9| 7| 4| 8| 7| 1| 8| 9| 6| 5| 3| 8| 9| 2| 5| 3| 2| 9| 2| | 1| 4| 2| 8| 6| 6| 5| 3| 9| 1| 1| 7| 1| 6| 0| 6| 7| 1| 7| 9| 6| 3| 4| 8| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 200 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + A| Adenocarcinoma, Metastatic, Lung | X | Hemangiosarcoma | X X X X X X X X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + A + + + + A| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Parathyroid Gland | + + + + M + + + + + M + + + M + + + + + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + A| Lymphoma Malignant Lymphocytic | X | Pars Intermedia, Adenoma | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + +| Follicular Cell, Adenoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Penis | | __________________________________________________________________________| Preputial Gland | + + + + + | Carcinoma | X X X X | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + A| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + M + + + + + + + + + + + A| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + A| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X | Mast Cell Tumor NOS | | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + M + + + + + + + + + + + + +| Axillary, Lymphoma Malignant | | Lymphocytic | X | Inguinal, Lymphoma Malignant | | _____________________________________________________________________________________________________________________| Page 151 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 6| 3| 5| 5| 5| 4| 6| 6| 2| 1| 2| 2| 5| 6| 4| 5| 2| 6| 6| 4| 7| 6| 5| 6| DAY ON TEST | 8| 7| 8| 6| 9| 9| 9| 7| 4| 8| 7| 1| 8| 9| 6| 5| 3| 8| 9| 2| 5| 3| 2| 9| 2| | 1| 4| 2| 8| 6| 6| 5| 3| 9| 1| 1| 7| 1| 6| 0| 6| 7| 1| 7| 9| 6| 3| 4| 8| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 200 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Lymphocytic | X | Renal, Lymphoma Malignant Lymphocytic| X | __________________________________________________________________________| Lymph Node, Bronchial | M + + + + + + M + M + M M + M + + + M + + + + + M| Adenocarcinoma, Metastatic, Lung | X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Lymph Node, Mandibular | I M + + + + + + M + + M M + + + + + + + + + + M +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Lymph Node, Mediastinal | M + + M + + + M + M + M M + + M + + + + I M + + M| Adenocarcinoma, Metastatic, Lung | X | Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + I + + + + + + + + + + + + A| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + A| Hemangiosarcoma | | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | | __________________________________________________________________________| Thymus | + + + + + I + + M + + M + + + + M + + + + + M + M| Adenocarcinoma, Metastatic, Lung | X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M + M M M M M M M M + + M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + +| Sebaceous Gland, Adenoma | | Subcutaneous Tissue, Adenocarcinoma, | | Metastatic, Lung | X | Subcutaneous Tissue, Hemangiosarcoma | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | + | Adenocarcinoma, Metastatic, Lung | X | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | _____________________________________________________________________________________________________________________| Page 152 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 6| 3| 5| 5| 5| 4| 6| 6| 2| 1| 2| 2| 5| 6| 4| 5| 2| 6| 6| 4| 7| 6| 5| 6| DAY ON TEST | 8| 7| 8| 6| 9| 9| 9| 7| 4| 8| 7| 1| 8| 9| 6| 5| 3| 8| 9| 2| 5| 3| 2| 9| 2| | 1| 4| 2| 8| 6| 6| 5| 3| 9| 1| 1| 7| 1| 6| 0| 6| 7| 1| 7| 9| 6| 3| 4| 8| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 200 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM - cont | | | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + +| Glioma Malignant | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X X X | Adenocarcinoma, Metastatic, | | Harderian Gland | | Alveolar/Bronchiolar Adenoma | X X X X X X | Alveolar/Bronchiolar Adenoma, | | Multiple | X X X X | Alveolar/Bronchiolar Carcinoma | X X X X X| Alveolar/Bronchiolar Carcinoma, | | Multiple | X X X X X X | Hemangiosarcoma, Metastatic, Heart | X X | Hepatocellular Carcinoma, Metastatic | X | Hepatocellular Carcinoma, Metastatic,| | Liver | X X X | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X | Mediastinum, Adenocarcinoma, | | Metastatic, Lung | X X | Mediastinum, Alveolar/Bronchiolar | | Carcinoma, Metastatic, Lung | X | Mediastinum, Hemangiosarcoma, | | Metastatic, Heart | X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + A| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + | __________________________________________________________________________| Harderian Gland | I + + + + + + + + + + M + + + + + M + + + + + + +| Adenocarcinoma | | Adenoma | X X X X X X X X X | Bilateral, Adenoma | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 153 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 6| 3| 5| 5| 5| 4| 6| 6| 2| 1| 2| 2| 5| 6| 4| 5| 2| 6| 6| 4| 7| 6| 5| 6| DAY ON TEST | 8| 7| 8| 6| 9| 9| 9| 7| 4| 8| 7| 1| 8| 9| 6| 5| 3| 8| 9| 2| 5| 3| 2| 9| 2| | 1| 4| 2| 8| 6| 6| 5| 3| 9| 1| 1| 7| 1| 6| 0| 6| 7| 1| 7| 9| 6| 3| 4| 8| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 200 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| URINARY SYSTEM - cont | | | | Adenocarcinoma, Metastatic, | | Harderian Gland | | Adenocarcinoma, Metastatic, Lung | | Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | | Renal Tubule, Adenoma | | __________________________________________________________________________| Urethra | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + A| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| Page 154 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 4| 5| 2| 5| 4| 4| 2| 4| 5| 2| 2| 7| 4| 5| 4| 7| 5| 2| 5| 2| 6| 3| 5| 6| DAY ON TEST | 1| 5| 6| 8| 6| 7| 5| 8| 5| 6| 8| 5| 3| 7| 9| 7| 3| 7| 8| 8| 8| 3| 7| 9| 2| | 0| 6| 8| 1| 9| 4| 6| 1| 6| 9| 8| 1| 3| 0| 5| 4| 3| 3| 1| 2| 1| 9| 1| 6| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 200 PPM | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + A + + + M + + + + + + +| __________________________________________________________________________| Gallbladder | A + + A + + + A + + A + + + + + A + A + + A| __________________________________________________________________________| Intestine Large | + + + + + + + A + + A + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | A + + + + + + A + + A + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | A + + + + + + A + + A + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + A + + A + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | A + + + + + + A + + A + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | A + + + + + + A + + A + + + + + + + A + + +| __________________________________________________________________________| Intestine Small, Ileum | A + + + + + + A + + A + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | A + + + + + + A + + A + + + + + A + A + + +| Carcinoma | X X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + A + + + + + + + + + + +| Adenocarcinoma, Metastatic, Lung | | Hemangiosarcoma, Metastatic, Heart | X X X X X | Hepatocellular Carcinoma | X X X X X X X X| Hepatocellular Carcinoma, Multiple | | Hepatocellular Adenoma | X X X X X X X| Hepatocellular Adenoma, Multiple | X X X X X | Hepatocholangiocarcinoma | | Histiocytic Sarcoma | X X X X | Lymphoma Malignant Lymphocytic | X | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | | __________________________________________________________________________| Mesentery | + + | Fat, Adenocarcinoma, Metastatic, Lung| | Fat, Hemangiosarcoma, Metastatic, | | Spleen | | Fat, Histiocytic Sarcoma | X | __________________________________________________________________________| Pancreas | + + + + + + + + + + A + + A + + + + + + + + + + +| Acinus, Histiocytic Sarcoma | | Duct, Adenoma, Multiple | | __________________________________________________________________________| Pharynx | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + A + + A + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + A + + A + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | | Squamous Cell Carcinoma | | Squamous Cell Papilloma | X X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + A + + A + + + + + + + + + + +| __________________________________________________________________________| Tooth | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | _____________________________________________________________________________________________________________________| Page 155 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 4| 5| 2| 5| 4| 4| 2| 4| 5| 2| 2| 7| 4| 5| 4| 7| 5| 2| 5| 2| 6| 3| 5| 6| DAY ON TEST | 1| 5| 6| 8| 6| 7| 5| 8| 5| 6| 8| 5| 3| 7| 9| 7| 3| 7| 8| 8| 8| 3| 7| 9| 2| | 0| 6| 8| 1| 9| 4| 6| 1| 6| 9| 8| 1| 3| 0| 5| 4| 3| 3| 1| 2| 1| 9| 1| 6| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 200 PPM | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + A + + + + + + + + + + +| Adenocarcinoma, Metastatic, Lung | | Hemangiosarcoma | X X X X X | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + A + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + A + + + + + + + + M + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + A + + A + + + + + + M + + + +| __________________________________________________________________________| Parathyroid Gland | + + + + + + M + + + A + + + + + + + + + + +| __________________________________________________________________________| Pituitary Gland | M + + + M + + + + + A + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | Pars Intermedia, Adenoma | X | __________________________________________________________________________| Thyroid Gland | + + + + + + + A + + A + + + + + + + + + + +| Follicular Cell, Adenoma | X| _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Penis | | __________________________________________________________________________| Preputial Gland | | Carcinoma | | __________________________________________________________________________| Prostate | + + + + + + + A + + A + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Seminal Vesicle | + + + + + + + A + + A + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + A + + + + + + + + + + +| Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X | Mast Cell Tumor NOS | | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + A + + + + + + + + + + +| Axillary, Lymphoma Malignant | | Lymphocytic | | Inguinal, Lymphoma Malignant | | _____________________________________________________________________________________________________________________| Page 156 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 4| 5| 2| 5| 4| 4| 2| 4| 5| 2| 2| 7| 4| 5| 4| 7| 5| 2| 5| 2| 6| 3| 5| 6| DAY ON TEST | 1| 5| 6| 8| 6| 7| 5| 8| 5| 6| 8| 5| 3| 7| 9| 7| 3| 7| 8| 8| 8| 3| 7| 9| 2| | 0| 6| 8| 1| 9| 4| 6| 1| 6| 9| 8| 1| 3| 0| 5| 4| 3| 3| 1| 2| 1| 9| 1| 6| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 200 PPM | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Lymphocytic | | Renal, Lymphoma Malignant Lymphocytic| | __________________________________________________________________________| Lymph Node, Bronchial | M M + + + + M M + + + + + M + M + + M + M + M + +| Adenocarcinoma, Metastatic, Lung | | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + M + + M + M M + + + + + + + M| Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Lymph Node, Mediastinal | + + + + + M M M M + + + + A + + M + + + M M + + +| Adenocarcinoma, Metastatic, Lung | | Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | | Histiocytic Sarcoma | X X X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + A + + + + + + + + + + +| Histiocytic Sarcoma | X X X X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Spleen | + + + + + + + + + + A + + A + + + + + + + + + + +| Hemangiosarcoma | | Histiocytic Sarcoma | X X X | Lymphoma Malignant Lymphocytic | X | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + A + + M + + + + M M + +| Adenocarcinoma, Metastatic, Lung | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M + M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + +| Sebaceous Gland, Adenoma | X | Subcutaneous Tissue, Adenocarcinoma, | | Metastatic, Lung | | Subcutaneous Tissue, Hemangiosarcoma | X| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | | Adenocarcinoma, Metastatic, Lung | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | _____________________________________________________________________________________________________________________| Page 157 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 4| 5| 2| 5| 4| 4| 2| 4| 5| 2| 2| 7| 4| 5| 4| 7| 5| 2| 5| 2| 6| 3| 5| 6| DAY ON TEST | 1| 5| 6| 8| 6| 7| 5| 8| 5| 6| 8| 5| 3| 7| 9| 7| 3| 7| 8| 8| 8| 3| 7| 9| 2| | 0| 6| 8| 1| 9| 4| 6| 1| 6| 9| 8| 1| 3| 0| 5| 4| 3| 3| 1| 2| 1| 9| 1| 6| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 200 PPM | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM - cont | | | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + +| Glioma Malignant | X | Histiocytic Sarcoma | X X X | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | A + + + + + + A + M A + + + + + + + A + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | | Adenocarcinoma, Metastatic, | | Harderian Gland | X | Alveolar/Bronchiolar Adenoma | X X X X X X X X | Alveolar/Bronchiolar Adenoma, | | Multiple | X| Alveolar/Bronchiolar Carcinoma | X X X X | Alveolar/Bronchiolar Carcinoma, | | Multiple | X X X X X | Hemangiosarcoma, Metastatic, Heart | X X X X | Hepatocellular Carcinoma, Metastatic | | Hepatocellular Carcinoma, Metastatic,| | Liver | | Histiocytic Sarcoma | X X X X | Lymphoma Malignant Lymphocytic | X | Mediastinum, Adenocarcinoma, | | Metastatic, Lung | | Mediastinum, Alveolar/Bronchiolar | | Carcinoma, Metastatic, Lung | | Mediastinum, Hemangiosarcoma, | | Metastatic, Heart | X | __________________________________________________________________________| Nose | + + + + + + + + + + A + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Trachea | A + + + + + + A + + A + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + + + + + + + | __________________________________________________________________________| Harderian Gland | + + + + + + + I + + M M + + + + + + I + M + + + +| Adenocarcinoma | X | Adenoma | X X X X X X X X X X | Bilateral, Adenoma | X X X X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 158 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 4| 5| 2| 5| 4| 4| 2| 4| 5| 2| 2| 7| 4| 5| 4| 7| 5| 2| 5| 2| 6| 3| 5| 6| DAY ON TEST | 1| 5| 6| 8| 6| 7| 5| 8| 5| 6| 8| 5| 3| 7| 9| 7| 3| 7| 8| 8| 8| 3| 7| 9| 2| | 0| 6| 8| 1| 9| 4| 6| 1| 6| 9| 8| 1| 3| 0| 5| 4| 3| 3| 1| 2| 1| 9| 1| 6| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 200 PPM | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| URINARY SYSTEM - cont | | | | Adenocarcinoma, Metastatic, | | Harderian Gland | | Adenocarcinoma, Metastatic, Lung | | Hemangiosarcoma, Metastatic, Heart | X | Histiocytic Sarcoma | X X X X | Lymphoma Malignant Lymphocytic | X | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | | Renal Tubule, Adenoma | X | __________________________________________________________________________| Urethra | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + A + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X X X | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| Page 159 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 5| 6| 5| 5| 4| 5| 7| 6| 4| 6| 4| 4| 3| 2| 5| 5| 4| 4| 4| 2| | | DAY ON TEST | 6| 5| 3| 8| 9| 1| 3| 2| 8| 9| 5| 5| 5| 8| 6| 2| 5| 5| 5| 8| | | | 8| 2| 0| 3| 5| 9| 3| 4| 1| 2| 6| 6| 1| 1| 8| 9| 6| 6| 6| 1| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 200 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + | 58 | __________________________________________________________________________|____________| Gallbladder | + + A + A + A + + + M + + M + | 42 | __________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + A + + + + | 56 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + A + + A + | 50 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + A + + + + | 55 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + A + + A + | 55 | __________________________________________________________________________|____________| Intestine Small | + + A + A + + + + + A + + A + | 49 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + A + A + + + + + A + + A + | 47 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + A + A + A + + + A + + A + | 47 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + A + A + A + A + A + + A + | 43 | Carcinoma | | 2 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + | 68 | Adenocarcinoma, Metastatic, Lung | | 1 | Hemangiosarcoma, Metastatic, Heart | X | 9 | Hepatocellular Carcinoma | X X X X | 18 | Hepatocellular Carcinoma, Multiple | X X X X | 9 | Hepatocellular Adenoma | X X X X X X X X X X | 22 | Hepatocellular Adenoma, Multiple | X X | 7 | Hepatocholangiocarcinoma | | 1 | Histiocytic Sarcoma | X | 7 | Lymphoma Malignant Lymphocytic | | 2 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | X | 1 | __________________________________________________________________________|____________| Mesentery | + + | 10 | Fat, Adenocarcinoma, Metastatic, Lung| | 1 | Fat, Hemangiosarcoma, Metastatic, | | | Spleen | X | 1 | Fat, Histiocytic Sarcoma | X | 3 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + A + + + + + + + | 66 | Acinus, Histiocytic Sarcoma | X | 1 | Duct, Adenoma, Multiple | | 1 | __________________________________________________________________________|____________| Pharynx | | 1 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + A + + + + | 58 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + | 68 | Lymphoma Malignant Lymphocytic | | 1 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | X | 1 | Squamous Cell Carcinoma | X | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 160 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 5| 6| 5| 5| 4| 5| 7| 6| 4| 6| 4| 4| 3| 2| 5| 5| 4| 4| 4| 2| | | DAY ON TEST | 6| 5| 3| 8| 9| 1| 3| 2| 8| 9| 5| 5| 5| 8| 6| 2| 5| 5| 5| 8| | | | 8| 2| 0| 3| 5| 9| 3| 4| 1| 2| 6| 6| 1| 1| 8| 9| 6| 6| 6| 1| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 200 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM - cont | | | | | | Squamous Cell Papilloma | X X | 7 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + A + + + + + + + A + + + + + + + | 65 | __________________________________________________________________________|____________| Tooth | | 1 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + | 68 | Adenocarcinoma, Metastatic, Lung | X | 2 | Hemangiosarcoma | X X X X X X X X | 21 | Histiocytic Sarcoma | | 3 | Lymphoma Malignant Lymphocytic | | 2 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + A + + + + | 58 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + A + + + + | 58 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + A + + A + | 54 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + A + + + + + + + | 65 | __________________________________________________________________________|____________| Parathyroid Gland | M M M + + M + + + M M + + M + | 48 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + | 56 | Lymphoma Malignant Lymphocytic | | 2 | Pars Intermedia, Adenoma | | 1 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + A + + + + | 57 | Follicular Cell, Adenoma | | 2 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + | 59 | __________________________________________________________________________|____________| Penis | + | 1 | __________________________________________________________________________|____________| Preputial Gland | + | 6 | Carcinoma | X | 5 | __________________________________________________________________________|____________| Prostate | + + + + + + + + + + A + + + + | 56 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + A + + A + | 54 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + | 69 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 161 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 5| 6| 5| 5| 4| 5| 7| 6| 4| 6| 4| 4| 3| 2| 5| 5| 4| 4| 4| 2| | | DAY ON TEST | 6| 5| 3| 8| 9| 1| 3| 2| 8| 9| 5| 5| 5| 8| 6| 2| 5| 5| 5| 8| | | | 8| 2| 0| 3| 5| 9| 3| 4| 1| 2| 6| 6| 1| 1| 8| 9| 6| 6| 6| 1| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 200 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | | | | __________________________________________________________________________|____________| Blood | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + | 69 | Histiocytic Sarcoma | | 4 | Lymphoma Malignant Lymphocytic | | 2 | Mast Cell Tumor NOS | X | 1 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + + + + + | 68 | Axillary, Lymphoma Malignant | | | Lymphocytic | | 1 | Inguinal, Lymphoma Malignant | | | Lymphocytic | | 1 | Renal, Lymphoma Malignant Lymphocytic| | 1 | __________________________________________________________________________|____________| Lymph Node, Bronchial | M + + M + + + M + + M + M M + + + + M + | 46 | Adenocarcinoma, Metastatic, Lung | X | 2 | Histiocytic Sarcoma | | 3 | Lymphoma Malignant Lymphocytic | | 2 | __________________________________________________________________________|____________| Lymph Node, Mandibular | I + + M + + + + + + + M + M + + M M + + | 53 | Histiocytic Sarcoma | | 2 | Lymphoma Malignant Lymphocytic | | 2 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | + + + + + + M + + + + M + + + + M + + M | 48 | Adenocarcinoma, Metastatic, Lung | | 1 | Alveolar/Bronchiolar Carcinoma, | | | Metastatic, Lung | | 1 | Histiocytic Sarcoma | | 4 | Lymphoma Malignant Lymphocytic | | 2 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + A + + + + + M + | 65 | Histiocytic Sarcoma | X | 6 | Lymphoma Malignant Lymphocytic | | 2 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + A + + + + + + + | 66 | Hemangiosarcoma | X | 1 | Histiocytic Sarcoma | X | 6 | Lymphoma Malignant Lymphocytic | | 2 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | X | 1 | __________________________________________________________________________|____________| Thymus | + M + + M + M M + M + + + + + + + + + + | 55 | Adenocarcinoma, Metastatic, Lung | | 1 | Histiocytic Sarcoma | | 2 | Lymphoma Malignant Lymphocytic | | 2 | Mast Cell Tumor NOS, Metastatic, | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 162 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 5| 6| 5| 5| 4| 5| 7| 6| 4| 6| 4| 4| 3| 2| 5| 5| 4| 4| 4| 2| | | DAY ON TEST | 6| 5| 3| 8| 9| 1| 3| 2| 8| 9| 5| 5| 5| 8| 6| 2| 5| 5| 5| 8| | | | 8| 2| 0| 3| 5| 9| 3| 4| 1| 2| 6| 6| 1| 1| 8| 9| 6| 6| 6| 1| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 200 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Bone Marrow | X | 1 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M + M M M M M M M M M M M M M | 5 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + | 60 | Sebaceous Gland, Adenoma | | 1 | Subcutaneous Tissue, Adenocarcinoma, | | | Metastatic, Lung | | 1 | Subcutaneous Tissue, Hemangiosarcoma | X X | 3 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + | 60 | __________________________________________________________________________|____________| Skeletal Muscle | + | 2 | Adenocarcinoma, Metastatic, Lung | X | 2 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + | 60 | Glioma Malignant | | 1 | Histiocytic Sarcoma | X | 5 | Lymphoma Malignant Lymphocytic | | 2 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + + + A + + + + | 53 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + | 70 | Adenocarcinoma | X | 4 | Adenocarcinoma, Metastatic, | | | Harderian Gland | X | 2 | Alveolar/Bronchiolar Adenoma | X X X X | 18 | Alveolar/Bronchiolar Adenoma, | | | Multiple | X X X | 8 | Alveolar/Bronchiolar Carcinoma | X X X | 12 | Alveolar/Bronchiolar Carcinoma, | | | Multiple | X | 12 | Hemangiosarcoma, Metastatic, Heart | X X | 8 | Hepatocellular Carcinoma, Metastatic | | 1 | Hepatocellular Carcinoma, Metastatic,| | | Liver | X | 4 | Histiocytic Sarcoma | X | 7 | Lymphoma Malignant Lymphocytic | | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 163 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 5| 6| 5| 5| 4| 5| 7| 6| 4| 6| 4| 4| 3| 2| 5| 5| 4| 4| 4| 2| | | DAY ON TEST | 6| 5| 3| 8| 9| 1| 3| 2| 8| 9| 5| 5| 5| 8| 6| 2| 5| 5| 5| 8| | | | 8| 2| 0| 3| 5| 9| 3| 4| 1| 2| 6| 6| 1| 1| 8| 9| 6| 6| 6| 1| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 200 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - cont | | | | | | Mediastinum, Adenocarcinoma, | | | Metastatic, Lung | X | 3 | Mediastinum, Alveolar/Bronchiolar | | | Carcinoma, Metastatic, Lung | | 1 | Mediastinum, Hemangiosarcoma, | | | Metastatic, Heart | X | 3 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + | 59 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | | 2 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + A + + + + | 55 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | M M | 8 | __________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + M | 61 | Adenocarcinoma | X | 2 | Adenoma | X X X X X X X | 26 | Bilateral, Adenoma | X X X | 7 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + A + + + + + + + | 69 | Adenocarcinoma, Metastatic, | | | Harderian Gland | X | 1 | Adenocarcinoma, Metastatic, Lung | X | 1 | Hemangiosarcoma, Metastatic, Heart | X | 2 | Histiocytic Sarcoma | | 6 | Lymphoma Malignant Lymphocytic | | 2 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | X | 1 | Renal Tubule, Adenoma | | 1 | __________________________________________________________________________|____________| Urethra | + + | 2 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + A + + + + + | 58 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + | 70 | Histiocytic Sarcoma | X | 7 | Lymphoma Malignant Lymphocytic | | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 164 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 1| 3| 2| 4| 4| 3| 2| 1| 2| 2| 2| 4| 2| 1| 2| 2| 2| 2| 2| 2| 3| 3| 2| 1| 3| DAY ON TEST | 8| 9| 8| 5| 5| 5| 1| 7| 9| 3| 3| 5| 8| 8| 9| 4| 4| 5| 6| 8| 2| 5| 3| 6| 1| | 4| 5| 1| 6| 6| 2| 2| 4| 7| 6| 9| 6| 6| 8| 1| 3| 3| 1| 8| 0| 7| 2| 2| 1| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 625 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + A A I A + M A + A + + + + A A + + + +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Intestine Large | + + + + + + + + + A + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + A + A + + A + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + A + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + A + + + + + + + + + + + + I + +| __________________________________________________________________________| Intestine Small | + + + + + + + A A A + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + M + + + + + A A A + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + A A A A + + A + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + A A A A + + I + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | | Hepatocellular Carcinoma | X | Hepatocellular Carcinoma, Multiple | | Hepatocellular Adenoma | X X| Hepatocellular Adenoma, Multiple | X X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X | Lymphoma Malignant Mixed | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Mesentery | + + + + | Squamous Cell Carcinoma, Metastatic, | | Stomach | | Fat, Hemangiosarcoma, Metastatic, | | Heart | | Fat, Squamous Cell Carcinoma, | | Metastatic, Stomach | X | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | Acinus, Histiocytic Sarcoma | | Acinus, Lymphoma Malignant | | Lymphocytic | X X X X X | Acinus, Squamous Cell Carcinoma, | | Metastatic, Stomach | | __________________________________________________________________________| Pharynx | + | Squamous Cell Papilloma | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + A + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X | _____________________________________________________________________________________________________________________| Page 165 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 1| 3| 2| 4| 4| 3| 2| 1| 2| 2| 2| 4| 2| 1| 2| 2| 2| 2| 2| 2| 3| 3| 2| 1| 3| DAY ON TEST | 8| 9| 8| 5| 5| 5| 1| 7| 9| 3| 3| 5| 8| 8| 9| 4| 4| 5| 6| 8| 2| 5| 3| 6| 1| | 4| 5| 1| 6| 6| 2| 2| 4| 7| 6| 9| 6| 6| 8| 1| 3| 3| 1| 8| 0| 7| 2| 2| 1| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 625 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM - cont | | | | __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X | Squamous Cell Carcinoma | X X | Squamous Cell Papilloma | X X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + I + + + + + + + + + + + M| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Blood Vessel | + | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X X| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Pheochromocytoma Benign | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + I + +| __________________________________________________________________________| Parathyroid Gland | + M + M + + + + + + + + + M M + + M + + M + + M +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Pituitary Gland | + + + + + + I + + + + + + + + I + + I + + + + + +| Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Coagulating Gland | + | __________________________________________________________________________| Epididymis | + + + + + + + + + A + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Penis | | _____________________________________________________________________________________________________________________| Page 166 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 1| 3| 2| 4| 4| 3| 2| 1| 2| 2| 2| 4| 2| 1| 2| 2| 2| 2| 2| 2| 3| 3| 2| 1| 3| DAY ON TEST | 8| 9| 8| 5| 5| 5| 1| 7| 9| 3| 3| 5| 8| 8| 9| 4| 4| 5| 6| 8| 2| 5| 3| 6| 1| | 4| 5| 1| 6| 6| 2| 2| 4| 7| 6| 9| 6| 6| 8| 1| 3| 3| 1| 8| 0| 7| 2| 2| 1| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 625 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| GENITAL SYSTEM - cont | | | | __________________________________________________________________________| Preputial Gland | | __________________________________________________________________________| Prostate | + + + + + + + + + A + + + + + + + + M + + + + + +| Lymphoma Malignant Lymphocytic | X X X | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + M + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | M | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X X| Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Axillary, Lymphoma Malignant | | Lymphocytic | X X X | Axillary, Lymphoma Malignant | | Iliac, Lymphoma Malignant Lymphocytic| X | Inguinal, Lymphoma Malignant | | Lymphocytic | | Lumbar, Lymphoma Malignant | | Lymphocytic | | Renal, Lymphoma Malignant Lymphocytic| X X | __________________________________________________________________________| Lymph Node, Bronchial | M M M M M + + M M M + M + + + + + M + + M + + + +| Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X| Lymphoma Malignant | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Lymph Node, Mandibular | + + M M M + + + M + + + + + + + + + M + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X | Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node, Mediastinal | M M M + + + + + + M + M + + + + + + + + + + M + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X| Lymphoma Malignant | | Lymphoma Malignant Mixed | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 167 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 1| 3| 2| 4| 4| 3| 2| 1| 2| 2| 2| 4| 2| 1| 2| 2| 2| 2| 2| 2| 3| 3| 2| 1| 3| DAY ON TEST | 8| 9| 8| 5| 5| 5| 1| 7| 9| 3| 3| 5| 8| 8| 9| 4| 4| 5| 6| 8| 2| 5| 3| 6| 1| | 4| 5| 1| 6| 6| 2| 2| 4| 7| 6| 9| 6| 6| 8| 1| 3| 3| 1| 8| 0| 7| 2| 2| 1| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 625 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X | Lymphoma Malignant | | Lymphoma Malignant Mixed | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X| Lymphoma Malignant Mixed | | __________________________________________________________________________| Thymus | + + + + M + + + + + + + + + + + + + + + M + + + +| Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X X| Lymphoma Malignant | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M I M M M M M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Histiocytic | | Sarcoma | | Subcutaneous Tissue, Lymphoma | | Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Skeletal Muscle | + | Diaphragm, Squamous Cell Carcinoma, | | Metastatic, Stomach | X | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X | __________________________________________________________________________| Spinal Cord | + + + | Lymphoma Malignant Lymphocytic | X X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | | Alveolar/Bronchiolar Adenoma | X X X | Alveolar/Bronchiolar Adenoma, | | Multiple | X | Alveolar/Bronchiolar Carcinoma | X X | _____________________________________________________________________________________________________________________| Page 168 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 1| 3| 2| 4| 4| 3| 2| 1| 2| 2| 2| 4| 2| 1| 2| 2| 2| 2| 2| 2| 3| 3| 2| 1| 3| DAY ON TEST | 8| 9| 8| 5| 5| 5| 1| 7| 9| 3| 3| 5| 8| 8| 9| 4| 4| 5| 6| 8| 2| 5| 3| 6| 1| | 4| 5| 1| 6| 6| 2| 2| 4| 7| 6| 9| 6| 6| 8| 1| 3| 3| 1| 8| 0| 7| 2| 2| 1| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 625 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X| Neoplasm NOS, Metastatic, Uncertain | | Primary Site | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Trachea | + + + + + + + + + A + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + +| __________________________________________________________________________| Harderian Gland | + + M + + + + + + I M + + M M M + + + M + + + M +| Adenoma | X X | Bilateral, Adenoma | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X| Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X | Squamous Cell Carcinoma, Metastatic, | | Stomach | | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X X X| Lymphoma Malignant Mixed | | _____________________________________________________________________________________________________________________| Page 169 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 2| 2| 3| 1| 2| 2| 2| 2| 4| 4| 4| 4| 1| 2| 2| 2| 2| 2| 1| 2| 3| 2| 1| 2| DAY ON TEST | 2| 2| 8| 7| 6| 1| 8| 5| 2| 5| 3| 1| 5| 8| 7| 7| 2| 5| 8| 6| 8| 0| 1| 8| 9| | 8| 7| 1| 0| 3| 6| 1| 8| 2| 6| 8| 4| 6| 2| 3| 7| 3| 7| 1| 7| 1| 6| 8| 0| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 625 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | A A + A + A + A A + + + M A + + A + + + + + A + M| Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Intestine Large | A A + A + + + + + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | A A + A + + + + + + + M + A + A + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | A A + A + + + + + + + + + A M + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | A A + A + + + + I + + + + A + + A + + + + + + + +| __________________________________________________________________________| Intestine Small | A A + A + + + + + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | A A + A + + + + + + A + + A + + I + + + + + M + +| Lymphoma Malignant Lymphocytic | X X X | __________________________________________________________________________| Intestine Small, Ileum | A A + A + + + + A + + + + A + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Intestine Small, Jejunum | A A + A + + + + A + A + + A + A A + + + + + + + +| Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Liver | + A + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | | Hepatocellular Carcinoma | | Hepatocellular Carcinoma, Multiple | X | Hepatocellular Adenoma | X X | Hepatocellular Adenoma, Multiple | X | Histiocytic Sarcoma | X X X| Lymphoma Malignant Lymphocytic | X X X X X X X X X X | Lymphoma Malignant Mixed | X | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | __________________________________________________________________________| Mesentery | + | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | Fat, Hemangiosarcoma, Metastatic, | | Heart | | Fat, Squamous Cell Carcinoma, | | Metastatic, Stomach | | __________________________________________________________________________| Pancreas | A + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | Acinus, Histiocytic Sarcoma | X| Acinus, Lymphoma Malignant | | Lymphocytic | X X X X X X X X | Acinus, Squamous Cell Carcinoma, | | Metastatic, Stomach | X | __________________________________________________________________________| Pharynx | | Squamous Cell Papilloma | | __________________________________________________________________________| Salivary Glands | A + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X| Lymphoma Malignant Lymphocytic | X X X X X | _____________________________________________________________________________________________________________________| Page 170 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 2| 2| 3| 1| 2| 2| 2| 2| 4| 4| 4| 4| 1| 2| 2| 2| 2| 2| 1| 2| 3| 2| 1| 2| DAY ON TEST | 2| 2| 8| 7| 6| 1| 8| 5| 2| 5| 3| 1| 5| 8| 7| 7| 2| 5| 8| 6| 8| 0| 1| 8| 9| | 8| 7| 1| 0| 3| 6| 1| 8| 2| 6| 8| 4| 6| 2| 3| 7| 3| 7| 1| 7| 1| 6| 8| 0| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 625 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM - cont | | | | __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + M +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + M +| Histiocytic Sarcoma | X| Lymphoma Malignant Lymphocytic | X X | Squamous Cell Carcinoma | X | Squamous Cell Papilloma | X | __________________________________________________________________________| Stomach, Glandular | A A + + + + + + + + + + + + + + M + + + + + + M +| Histiocytic Sarcoma | X| Lymphoma Malignant Lymphocytic | X X X X X | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Blood Vessel | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X X | __________________________________________________________________________| Adrenal Gland, Medulla | A + + A + + + + + + + + + + + + + + + + + + M + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | Pheochromocytoma Benign | | __________________________________________________________________________| Islets, Pancreatic | A + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | + + + + M + M + + + + + + + + I M + + M + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Pituitary Gland | + + + + + M + + + + M + + + + + + + + M + + + + +| Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Thyroid Gland | A + + + + + M + + + + + + A + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Coagulating Gland | + | __________________________________________________________________________| Epididymis | + + M + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X| Lymphoma Malignant Lymphocytic | X X X | __________________________________________________________________________| Penis | + M | _____________________________________________________________________________________________________________________| Page 171 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 2| 2| 3| 1| 2| 2| 2| 2| 4| 4| 4| 4| 1| 2| 2| 2| 2| 2| 1| 2| 3| 2| 1| 2| DAY ON TEST | 2| 2| 8| 7| 6| 1| 8| 5| 2| 5| 3| 1| 5| 8| 7| 7| 2| 5| 8| 6| 8| 0| 1| 8| 9| | 8| 7| 1| 0| 3| 6| 1| 8| 2| 6| 8| 4| 6| 2| 3| 7| 3| 7| 1| 7| 1| 6| 8| 0| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 625 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| GENITAL SYSTEM - cont | | | | __________________________________________________________________________| Preputial Gland | + | __________________________________________________________________________| Prostate | A A + + + + + + + + + + + + + M + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Seminal Vesicle | A A + A + + + + + + + + + + + M + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + M + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X X| Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X| Lymphoma Malignant Lymphocytic | X | Axillary, Lymphoma Malignant | | Lymphocytic | X | Axillary, Lymphoma Malignant | X | Iliac, Lymphoma Malignant Lymphocytic| X | Inguinal, Lymphoma Malignant | | Lymphocytic | X | Lumbar, Lymphoma Malignant | | Lymphocytic | X | Renal, Lymphoma Malignant Lymphocytic| X X | __________________________________________________________________________| Lymph Node, Bronchial | + + M + M + M I + M M + M + M + + + + + M + + + M| Lymphoma Malignant Lymphocytic | X X X X X X X X X X X | Lymphoma Malignant | X | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | __________________________________________________________________________| Lymph Node, Mandibular | A + I M + + + M + M + + + + + + + + M + M + M + +| Histiocytic Sarcoma | X X| Lymphoma Malignant Lymphocytic | X X X X X X X X X | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Lymph Node, Mediastinal | + + + + M + + M + + + + + + + + + I M + + + + M M| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X | Lymphoma Malignant | X | Lymphoma Malignant Mixed | X | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | __________________________________________________________________________| Lymph Node, Mesenteric | + M + + + + + + + + M + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 172 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 2| 2| 3| 1| 2| 2| 2| 2| 4| 4| 4| 4| 1| 2| 2| 2| 2| 2| 1| 2| 3| 2| 1| 2| DAY ON TEST | 2| 2| 8| 7| 6| 1| 8| 5| 2| 5| 3| 1| 5| 8| 7| 7| 2| 5| 8| 6| 8| 0| 1| 8| 9| | 8| 7| 1| 0| 3| 6| 1| 8| 2| 6| 8| 4| 6| 2| 3| 7| 3| 7| 1| 7| 1| 6| 8| 0| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 625 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Histiocytic Sarcoma | X X X| Lymphoma Malignant Lymphocytic | X X X X X X X X X | Lymphoma Malignant | X | Lymphoma Malignant Mixed | X | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | __________________________________________________________________________| Spleen | A A + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X X| Lymphoma Malignant Lymphocytic | X X X X X X X X X X X | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Thymus | + + + + + + M + M + M + + + M + + I + + + + + + M| Lymphoma Malignant Lymphocytic | X X X X X X X X X X X | Lymphoma Malignant | X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M + M M + M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Histiocytic | | Sarcoma | X | Subcutaneous Tissue, Lymphoma | | Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Skeletal Muscle | | Diaphragm, Squamous Cell Carcinoma, | | Metastatic, Stomach | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Spinal Cord | + + + | Lymphoma Malignant Lymphocytic | X X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | A + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | | Alveolar/Bronchiolar Adenoma | | Alveolar/Bronchiolar Adenoma, | | Multiple | X | Alveolar/Bronchiolar Carcinoma | X | _____________________________________________________________________________________________________________________| Page 173 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 2| 2| 3| 1| 2| 2| 2| 2| 4| 4| 4| 4| 1| 2| 2| 2| 2| 2| 1| 2| 3| 2| 1| 2| DAY ON TEST | 2| 2| 8| 7| 6| 1| 8| 5| 2| 5| 3| 1| 5| 8| 7| 7| 2| 5| 8| 6| 8| 0| 1| 8| 9| | 8| 7| 1| 0| 3| 6| 1| 8| 2| 6| 8| 4| 6| 2| 3| 7| 3| 7| 1| 7| 1| 6| 8| 0| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 625 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | X X| Lymphoma Malignant Lymphocytic | X X X X X X X X | Neoplasm NOS, Metastatic, Uncertain | | Primary Site | | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | __________________________________________________________________________| Nose | + A + + + + + + + + + + + A + + + + + + + + + + +| Histiocytic Sarcoma | X| Lymphoma Malignant Lymphocytic | X X X X | __________________________________________________________________________| Trachea | A + + + + + + + + + + + + A + I + + + + + + + + +| Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | | __________________________________________________________________________| Harderian Gland | + M + + M + + M M + + + + M + M M M M M + + M + +| Adenoma | X | Bilateral, Adenoma | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | Histiocytic Sarcoma | X X X| Lymphoma Malignant Lymphocytic | X X X X X X X | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | __________________________________________________________________________| Urinary Bladder | A A + + + + + + + + + + + + + M + + + + + + + + +| Histiocytic Sarcoma | X X| Lymphoma Malignant Lymphocytic | X X X X X | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X X| Lymphoma Malignant | X | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X | Lymphoma Malignant Mixed | X | _____________________________________________________________________________________________________________________| Page 174 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 3| 2| 2| 2| 2| 2| 4| 2| 2| 2| 4| 3| 2| 2| 2| 1| 4| 3| 2| 2| 2| 1| 2| 2| DAY ON TEST | 1| 1| 4| 5| 8| 8| 4| 2| 4| 5| 8| 5| 0| 8| 9| 8| 9| 5| 7| 1| 4| 6| 9| 2| 8| | 2| 9| 3| 0| 1| 1| 9| 2| 3| 9| 1| 6| 2| 9| 5| 5| 9| 6| 6| 5| 7| 4| 2| 8| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 625 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + M + + A + + + + + A A + + A + + M + + A A +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + A + + + + + + + + A + +| __________________________________________________________________________| Intestine Large, Cecum | + + + A + + + + + A + + + A + + A + + A + + A + +| __________________________________________________________________________| Intestine Large, Colon | + + + A + + + + + + + + + A + + + + + A + + A + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + M A + + + + + + + + A + +| __________________________________________________________________________| Intestine Small | + + + A + + + + + A + + + A + + A + + A + A A + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + A + + + + + A + + A A + + M + + A + A A A +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Intestine Small, Ileum | + + + A + + + + + A + + I A + + A + + A + A A I +| Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Intestine Small, Jejunum | + + + A + + A + + A + + + A + + A + A A + M A + +| Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | X X | Hepatocellular Carcinoma | X X | Hepatocellular Carcinoma, Multiple | | Hepatocellular Adenoma | X | Hepatocellular Adenoma, Multiple | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X| Lymphoma Malignant Mixed | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Mesentery | + | Squamous Cell Carcinoma, Metastatic, | | Stomach | | Fat, Hemangiosarcoma, Metastatic, | | Heart | X | Fat, Squamous Cell Carcinoma, | | Metastatic, Stomach | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | Acinus, Histiocytic Sarcoma | | Acinus, Lymphoma Malignant | | Lymphocytic | X X X X X X| Acinus, Squamous Cell Carcinoma, | | Metastatic, Stomach | | __________________________________________________________________________| Pharynx | + | Squamous Cell Papilloma | X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + A + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X| _____________________________________________________________________________________________________________________| Page 175 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 3| 2| 2| 2| 2| 2| 4| 2| 2| 2| 4| 3| 2| 2| 2| 1| 4| 3| 2| 2| 2| 1| 2| 2| DAY ON TEST | 1| 1| 4| 5| 8| 8| 4| 2| 4| 5| 8| 5| 0| 8| 9| 8| 9| 5| 7| 1| 4| 6| 9| 2| 8| | 2| 9| 3| 0| 1| 1| 9| 2| 3| 9| 1| 6| 2| 9| 5| 5| 9| 6| 6| 5| 7| 4| 2| 8| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 625 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM - cont | | | | __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X| Squamous Cell Carcinoma | X | Squamous Cell Papilloma | X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + A + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X | Squamous Cell Carcinoma, Metastatic, | | Stomach | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Blood Vessel | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X X X X | Lymphoma Malignant Lymphocytic | X X X X X| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X X | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X | Pheochromocytoma Benign | X | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | I + + M + + + + + + + M + M + + M + + M + + M + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Pituitary Gland | + + + + + + + + + I + M + I + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + A + +| Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Coagulating Gland | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + M + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Penis | | _____________________________________________________________________________________________________________________| Page 176 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 3| 2| 2| 2| 2| 2| 4| 2| 2| 2| 4| 3| 2| 2| 2| 1| 4| 3| 2| 2| 2| 1| 2| 2| DAY ON TEST | 1| 1| 4| 5| 8| 8| 4| 2| 4| 5| 8| 5| 0| 8| 9| 8| 9| 5| 7| 1| 4| 6| 9| 2| 8| | 2| 9| 3| 0| 1| 1| 9| 2| 3| 9| 1| 6| 2| 9| 5| 5| 9| 6| 6| 5| 7| 4| 2| 8| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 625 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| GENITAL SYSTEM - cont | | | | __________________________________________________________________________| Preputial Gland | + | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + A M + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X| __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + A + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X| Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | | Axillary, Lymphoma Malignant | | Lymphocytic | X X X| Axillary, Lymphoma Malignant | | Iliac, Lymphoma Malignant Lymphocytic| X | Inguinal, Lymphoma Malignant | | Lymphocytic | X | Lumbar, Lymphoma Malignant | | Lymphocytic | | Renal, Lymphoma Malignant Lymphocytic| X X | __________________________________________________________________________| Lymph Node, Bronchial | + + + M + M M + + M + M + + M M + M M M + M + + +| Lymphoma Malignant Lymphocytic | X X X X X X X X X X| Lymphoma Malignant | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + M + + + + + + I M + + + + + + + M A + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X| Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node, Mediastinal | + + + M M + + + M + M + + + + + + + + M + M M + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X| Lymphoma Malignant | | Lymphoma Malignant Mixed | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + I + + + + + + M + + + + + + A + +| _____________________________________________________________________________________________________________________| Page 177 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 3| 2| 2| 2| 2| 2| 4| 2| 2| 2| 4| 3| 2| 2| 2| 1| 4| 3| 2| 2| 2| 1| 2| 2| DAY ON TEST | 1| 1| 4| 5| 8| 8| 4| 2| 4| 5| 8| 5| 0| 8| 9| 8| 9| 5| 7| 1| 4| 6| 9| 2| 8| | 2| 9| 3| 0| 1| 1| 9| 2| 3| 9| 1| 6| 2| 9| 5| 5| 9| 6| 6| 5| 7| 4| 2| 8| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 625 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X| Lymphoma Malignant | | Lymphoma Malignant Mixed | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + A + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X X| Lymphoma Malignant Mixed | | __________________________________________________________________________| Thymus | + + + + M + I + + + + + + + + + + + + M + + A + +| Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X| Lymphoma Malignant | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M + + M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Histiocytic | | Sarcoma | | Subcutaneous Tissue, Lymphoma | | Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Skeletal Muscle | | Diaphragm, Squamous Cell Carcinoma, | | Metastatic, Stomach | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X| __________________________________________________________________________| Spinal Cord | + +| Lymphoma Malignant Lymphocytic | X X| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + M + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X | Alveolar/Bronchiolar Adenoma | X X X | Alveolar/Bronchiolar Adenoma, | | Multiple | X | Alveolar/Bronchiolar Carcinoma | X X | _____________________________________________________________________________________________________________________| Page 178 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 4| 3| 2| 2| 2| 2| 2| 4| 2| 2| 2| 4| 3| 2| 2| 2| 1| 4| 3| 2| 2| 2| 1| 2| 2| DAY ON TEST | 1| 1| 4| 5| 8| 8| 4| 2| 4| 5| 8| 5| 0| 8| 9| 8| 9| 5| 7| 1| 4| 6| 9| 2| 8| | 2| 9| 3| 0| 1| 1| 9| 2| 3| 9| 1| 6| 2| 9| 5| 5| 9| 6| 6| 5| 7| 4| 2| 8| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 625 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Hemangiosarcoma, Metastatic, Heart | X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X X| Neoplasm NOS, Metastatic, Uncertain | | Primary Site | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X| __________________________________________________________________________| Trachea | + + + + + + + + + I + + + + + + + + + + + + I + +| Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + + + | __________________________________________________________________________| Harderian Gland | + + M M + M M + M M + + + + + + M + + M M M M + +| Adenoma | X X X X| Bilateral, Adenoma | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X X| Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + A + + A + + + + + A + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X | Squamous Cell Carcinoma, Metastatic, | | Stomach | | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X X X| Lymphoma Malignant Mixed | | _____________________________________________________________________________________________________________________| Page 179 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 3| 1| 1| 2| 2| 2| 2| 2| 2| 3| 2| 2| | | DAY ON TEST | 6| 3| 7| 1| 8| 2| 8| 8| 4| 2| 5| 3| 1| 6| 6| | | | 0| 9| 1| 0| 7| 0| 1| 1| 0| 2| 4| 6| 5| 4| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | A | 625 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + | 90 | __________________________________________________________________________|____________| Gallbladder | + + A + + A + + + + A + + A M | 57 | Lymphoma Malignant Lymphocytic | | 3 | __________________________________________________________________________|____________| Intestine Large | + + A + + + + + + + + + + + + | 82 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + A + + A + + + + A + + + + | 72 | __________________________________________________________________________|____________| Intestine Large, Colon | + + A + + A + + + + + + + + + | 78 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + A + + + + + + + + + + + + | 78 | __________________________________________________________________________|____________| Intestine Small | + + + + + A + + + + + + + + + | 75 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + A + + A M + + + + + + + + | 67 | Lymphoma Malignant Lymphocytic | X | 4 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + A + + A + + + + A + + + + | 68 | Lymphoma Malignant Lymphocytic | | 3 | __________________________________________________________________________|____________| Intestine Small, Jejunum | A + + + + A + + + + + + + A + | 65 | Lymphoma Malignant Lymphocytic | | 4 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 89 | Hemangiosarcoma, Metastatic, Heart | X | 3 | Hepatocellular Carcinoma | | 3 | Hepatocellular Carcinoma, Multiple | | 1 | Hepatocellular Adenoma | X | 6 | Hepatocellular Adenoma, Multiple | | 3 | Histiocytic Sarcoma | X | 4 | Lymphoma Malignant Lymphocytic | X X X X X X X X X | 42 | Lymphoma Malignant Mixed | | 1 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | | 1 | __________________________________________________________________________|____________| Mesentery | | 6 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | | 1 | Fat, Hemangiosarcoma, Metastatic, | | | Heart | | 1 | Fat, Squamous Cell Carcinoma, | | | Metastatic, Stomach | | 1 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + | 89 | Lymphoma Malignant Lymphocytic | | 1 | Acinus, Histiocytic Sarcoma | X | 2 | Acinus, Lymphoma Malignant | | | Lymphocytic | X X X X | 23 | Acinus, Squamous Cell Carcinoma, | | | Metastatic, Stomach | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 180 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 3| 1| 1| 2| 2| 2| 2| 2| 2| 3| 2| 2| | | DAY ON TEST | 6| 3| 7| 1| 8| 2| 8| 8| 4| 2| 5| 3| 1| 6| 6| | | | 0| 9| 1| 0| 7| 0| 1| 1| 0| 2| 4| 6| 5| 4| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | A | 625 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Pharynx | | 2 | Squamous Cell Papilloma | | 1 | __________________________________________________________________________|____________| Salivary Glands | + + + + + M + + + + + + + + + | 86 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X X X | 20 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + | 89 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + | 89 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X X | 9 | Squamous Cell Carcinoma | | 4 | Squamous Cell Papilloma | | 4 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + | 83 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X X | 10 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | | 2 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Blood Vessel | | 1 | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 90 | Hemangiosarcoma | X | 7 | Lymphoma Malignant Lymphocytic | X X | 20 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + + | 90 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + | 90 | Lymphoma Malignant Lymphocytic | | 6 | __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + M + + + + + + + + + | 86 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | | 3 | Pheochromocytoma Benign | | 1 | __________________________________________________________________________|____________| Islets, Pancreatic | + + I + + + + + + + + + + + + | 87 | __________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + M + + M + + + | 69 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Pituitary Gland | + + + I + + + + + M + + M + + | 78 | Lymphoma Malignant Lymphocytic | X | 3 | Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + | 86 | Lymphoma Malignant Lymphocytic | X | 5 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 181 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 3| 1| 1| 2| 2| 2| 2| 2| 2| 3| 2| 2| | | DAY ON TEST | 6| 3| 7| 1| 8| 2| 8| 8| 4| 2| 5| 3| 1| 6| 6| | | | 0| 9| 1| 0| 7| 0| 1| 1| 0| 2| 4| 6| 5| 4| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | A | 625 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Coagulating Gland | | 2 | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + | 87 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X | 6 | __________________________________________________________________________|____________| Penis | | 1 | __________________________________________________________________________|____________| Preputial Gland | | 2 | __________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + | 83 | Lymphoma Malignant Lymphocytic | X X | 9 | __________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + | 84 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | | 7 | __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + | 89 | Lymphoma Malignant Lymphocytic | | 5 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Blood | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + | 90 | Histiocytic Sarcoma | X | 4 | Lymphoma Malignant Lymphocytic | X X X X X X X X X | 46 | Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + | 90 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X | 2 | Axillary, Lymphoma Malignant | | | Lymphocytic | X | 8 | Axillary, Lymphoma Malignant | | 1 | Iliac, Lymphoma Malignant Lymphocytic| | 3 | Inguinal, Lymphoma Malignant | | | Lymphocytic | | 2 | Lumbar, Lymphoma Malignant | | | Lymphocytic | | 1 | Renal, Lymphoma Malignant Lymphocytic| X | 7 | __________________________________________________________________________|____________| Lymph Node, Bronchial | + M M M + M M + + + + + + + + | 53 | Lymphoma Malignant Lymphocytic | X X X X X X X | 40 | Lymphoma Malignant | | 1 | Squamous Cell Carcinoma, Metastatic, | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 182 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 3| 1| 1| 2| 2| 2| 2| 2| 2| 3| 2| 2| | | DAY ON TEST | 6| 3| 7| 1| 8| 2| 8| 8| 4| 2| 5| 3| 1| 6| 6| | | | 0| 9| 1| 0| 7| 0| 1| 1| 0| 2| 4| 6| 5| 4| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | A | 625 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Stomach | | 1 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + M + + + + + + M + + | 70 | Histiocytic Sarcoma | | 2 | Lymphoma Malignant Lymphocytic | X X X X X X X | 40 | Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | + + + M + + M M + + + + + + + | 68 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant Lymphocytic | X X X X X X X X X | 45 | Lymphoma Malignant | | 1 | Lymphoma Malignant Mixed | | 1 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | | 1 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + M + + + + + + + | 84 | Histiocytic Sarcoma | | 3 | Lymphoma Malignant Lymphocytic | X X X X X X X X | 42 | Lymphoma Malignant | | 1 | Lymphoma Malignant Mixed | | 1 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | | 1 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + | 87 | Histiocytic Sarcoma | X | 4 | Lymphoma Malignant Lymphocytic | X X X X X X X X X | 45 | Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________|____________| Thymus | + + + + + + + + + + + + + + + | 78 | Lymphoma Malignant Lymphocytic | X X X X X X X X | 45 | Lymphoma Malignant | | 1 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M I M + M + | 6 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + | 90 | Subcutaneous Tissue, Histiocytic | | | Sarcoma | | 1 | Subcutaneous Tissue, Lymphoma | | | Malignant Lymphocytic | | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + | 90 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Skeletal Muscle | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 183 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 3| 1| 1| 2| 2| 2| 2| 2| 2| 3| 2| 2| | | DAY ON TEST | 6| 3| 7| 1| 8| 2| 8| 8| 4| 2| 5| 3| 1| 6| 6| | | | 0| 9| 1| 0| 7| 0| 1| 1| 0| 2| 4| 6| 5| 4| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | A | 625 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ MUSCULOSKELETAL SYSTEM - cont | | | | | | Diaphragm, Squamous Cell Carcinoma, | | | Metastatic, Stomach | | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + | 90 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X | 10 | __________________________________________________________________________|____________| Spinal Cord | + + + + | 12 | Lymphoma Malignant Lymphocytic | X X X | 9 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + + + + + + + + | 88 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + | 90 | Adenocarcinoma | | 1 | Alveolar/Bronchiolar Adenoma | | 6 | Alveolar/Bronchiolar Adenoma, | | | Multiple | X | 4 | Alveolar/Bronchiolar Carcinoma | | 5 | Hemangiosarcoma, Metastatic, Heart | X | 2 | Histiocytic Sarcoma | X | 3 | Lymphoma Malignant Lymphocytic | X X X X | 32 | Neoplasm NOS, Metastatic, Uncertain | | | Primary Site | X | 1 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | | 1 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + | 88 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X | 8 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + | 84 | Lymphoma Malignant Lymphocytic | X | 3 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | + | 6 | __________________________________________________________________________|____________| Harderian Gland | + + M + M M M + M M M + + + + | 53 | Adenoma | | 7 | Bilateral, Adenoma | | 2 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + | 90 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 184 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 3| 1| 1| 2| 2| 2| 2| 2| 2| 3| 2| 2| | | DAY ON TEST | 6| 3| 7| 1| 8| 2| 8| 8| 4| 2| 5| 3| 1| 6| 6| | | | 0| 9| 1| 0| 7| 0| 1| 1| 0| 2| 4| 6| 5| 4| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | A | 625 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ URINARY SYSTEM - cont | | | | | | Adenoma | | 1 | Histiocytic Sarcoma | X | 4 | Lymphoma Malignant Lymphocytic | X X X | 29 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | | 1 | __________________________________________________________________________|____________| Urinary Bladder | + + A + + + + + + + + + + + + | 83 | Histiocytic Sarcoma | | 2 | Lymphoma Malignant Lymphocytic | X X X | 16 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | | 1 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 90 | Histiocytic Sarcoma | X | 4 | Lymphoma Malignant | | 1 | Lymphoma Malignant Lymphocytic | X X X X X X X X X | 52 | Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 185 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 5| 5| 7| 6| 5| 5| 3| 5| 7| 5| 7| 6| 3| 4| 2| 5| 6| 7| 2| 6| 7| 7| 4| 5| DAY ON TEST | 9| 7| 1| 2| 9| 5| 7| 3| 6| 2| 8| 2| 2| 9| 6| 0| 1| 9| 2| 6| 5| 2| 2| 8| 1| | 6| 6| 6| 9| 9| 5| 6| 0| 7| 9| 4| 9| 3| 9| 8| 8| 0| 8| 9| 2| 1| 9| 9| 9| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 200 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE40 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + M| __________________________________________________________________________| Gallbladder | I + A + + + + + + + A + + + A A + + + A + + + A +| __________________________________________________________________________| Intestine Large | + + A + + + + + + + + + + + A A + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + A + + + + + + + A + + + A A + + + A + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + A + + + + + + + + + + + A A + + + A + + + + +| Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | __________________________________________________________________________| Intestine Large, Rectum | + + A + + + + + + + + + + + A A + + + + + + + + M| __________________________________________________________________________| Intestine Small | + + A + + + + + + + A + + + + A + + + A + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + A + + + + + + + A + + + A A + + + M + + + + +| Lymphoma Malignant Mixed | | __________________________________________________________________________| Intestine Small, Ileum | + + A + + + + + + + A + + + + A + + + A + + + + +| Histiocytic Sarcoma | X | __________________________________________________________________________| Intestine Small, Jejunum | + + A + + + + + + + A + + + A A + + + A + + + + +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + A + + + + + + + + +| Hemangioma | X | Hemangiosarcoma, Metastatic, Heart | X X X X | Hepatocellular Carcinoma | X X X X | Hepatocellular Carcinoma, Multiple | X X X X| Hepatocellular Adenoma | X X X X X X X | Hepatocellular Adenoma, Multiple | X X X X X | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X X | __________________________________________________________________________| Mesentery | + + + + + + | Fat, Hemangioma | X | Fat, Hemangioma, Multiple | X | Fat, Hemangiosarcoma | | Fat, Hemangiosarcoma, Metastatic, | | Heart | X X | Fat, Mast Cell Tumor NOS, Metastatic,| | Bone Marrow | X X | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + A A + + + + + + + + +| Acinus, Histiocytic Sarcoma | X X | Acinus, Lymphoma Malignant | | Lymphocytic | X | __________________________________________________________________________| Pharynx | + | Palate, Mast Cell Tumor NOS, | | Metastatic, Bone Marrow | X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + A + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + A + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + A + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 186 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 5| 5| 7| 6| 5| 5| 3| 5| 7| 5| 7| 6| 3| 4| 2| 5| 6| 7| 2| 6| 7| 7| 4| 5| DAY ON TEST | 9| 7| 1| 2| 9| 5| 7| 3| 6| 2| 8| 2| 2| 9| 6| 0| 1| 9| 2| 6| 5| 2| 2| 8| 1| | 6| 6| 6| 9| 9| 5| 6| 0| 7| 9| 4| 9| 3| 9| 8| 8| 0| 8| 9| 2| 1| 9| 9| 9| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 200 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE40 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM - cont | | | | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X X | Squamous Cell Papilloma | X X | Squamous Cell Papilloma, Multiple | X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + A A + + + A + + + A +| Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | __________________________________________________________________________| Tooth | + + + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X X X X X X X X X | Lymphoma Malignant Lymphocytic | X X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + M + + A + + + + + + + A +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + M + + A + + + + + + + A +| Adenoma | X | Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + M + + A + + + + + + + A +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + A A + + + + + + + + +| Adenoma | X | __________________________________________________________________________| Parathyroid Gland | + + + + I + + + + + + + + + M A + M + + + M + + +| __________________________________________________________________________| Pituitary Gland | + + I + + I + + + + + + + + + A + + + + + + M + +| Pars Intermedia, Adenoma | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + A + + + + + + + + +| Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | Follicular Cell, Adenoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + A + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | X | __________________________________________________________________________| Penis | + +| __________________________________________________________________________| Preputial Gland | + + + + + +| Bilateral, Carcinoma | X | __________________________________________________________________________| Prostate | + + M + + + + + + + + + + + A A + + + + + + + A +| __________________________________________________________________________| Seminal Vesicle | + + A + + + + + + + + + + + A A + + + A + + + + +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 187 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 5| 5| 7| 6| 5| 5| 3| 5| 7| 5| 7| 6| 3| 4| 2| 5| 6| 7| 2| 6| 7| 7| 4| 5| DAY ON TEST | 9| 7| 1| 2| 9| 5| 7| 3| 6| 2| 8| 2| 2| 9| 6| 0| 1| 9| 2| 6| 5| 2| 2| 8| 1| | 6| 6| 6| 9| 9| 5| 6| 0| 7| 9| 4| 9| 3| 9| 8| 8| 0| 8| 9| 2| 1| 9| 9| 9| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 200 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE40 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| GENITAL SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X | Mast Cell Tumor NOS | X X | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | Renal, Lymphoma Malignant Lymphocytic| X X X | __________________________________________________________________________| Lymph Node, Bronchial | M + + + M + M + + + + + M + + + + M + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + A + + + + + M + + M| Histiocytic Sarcoma | X | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X X | __________________________________________________________________________| Lymph Node, Mediastinal | + + + + + + + + + M + + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X | Lymphoma Malignant | | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + A M + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant | | Lymphoma Malignant Mixed | | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | __________________________________________________________________________| Spleen | + + A + + + + + + + + + + + + A + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | X | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X X | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X X | __________________________________________________________________________| Thymus | M + + + M + + M M + + + + + + + + + + + + + M + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X | Lymphoma Malignant | | Mast Cell Tumor NOS, Metastatic, | | _____________________________________________________________________________________________________________________| Page 188 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 5| 5| 7| 6| 5| 5| 3| 5| 7| 5| 7| 6| 3| 4| 2| 5| 6| 7| 2| 6| 7| 7| 4| 5| DAY ON TEST | 9| 7| 1| 2| 9| 5| 7| 3| 6| 2| 8| 2| 2| 9| 6| 0| 1| 9| 2| 6| 5| 2| 2| 8| 1| | 6| 6| 6| 9| 9| 5| 6| 0| 7| 9| 4| 9| 3| 9| 8| 8| 0| 8| 9| 2| 1| 9| 9| 9| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 200 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE40 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Bone Marrow | X X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M + + M M M M M M M + M M M M M M +| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | Subcutaneous Tissue, Hemangioma | | Subcutaneous Tissue, Hemangiosarcoma | | Subcutaneous Tissue, Hemangiosarcoma,| | Metastatic, Heart | X X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + A + + + + + + + + + + + + A + + + I + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, | | Harderian Gland | X X | Alveolar/Bronchiolar Adenoma | X X X X X X X X X| Alveolar/Bronchiolar Adenoma, | | Multiple | X X X X | Alveolar/Bronchiolar Carcinoma | X X X X X X | Alveolar/Bronchiolar Carcinoma, | | Multiple | X X | Hemangiosarcoma, Metastatic, Heart | X X X | Hepatocellular Carcinoma, Metastatic,| | Liver | X | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X X X | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | __________________________________________________________________________| Nose | + + A + + + + + + + + + + + + + + + + + + + + + +| Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | __________________________________________________________________________| Trachea | + + A + + + + + + + + + + + + A + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | _____________________________________________________________________________________________________________________| Page 189 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 5| 5| 7| 6| 5| 5| 3| 5| 7| 5| 7| 6| 3| 4| 2| 5| 6| 7| 2| 6| 7| 7| 4| 5| DAY ON TEST | 9| 7| 1| 2| 9| 5| 7| 3| 6| 2| 8| 2| 2| 9| 6| 0| 1| 9| 2| 6| 5| 2| 2| 8| 1| | 6| 6| 6| 9| 9| 5| 6| 0| 7| 9| 4| 9| 3| 9| 8| 8| 0| 8| 9| 2| 1| 9| 9| 9| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 200 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE40 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM - cont | | | | | | __________________________________________________________________________| Ear | | __________________________________________________________________________| Eye | | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + M + + + M + + + + +| Adenocarcinoma | X X | Adenoma | X X X X X X X X X X X| Bilateral, Adenoma | X X X | __________________________________________________________________________| Zymbal's Gland | | Carcinoma | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + A + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | X | Hemangiosarcoma, Metastatic, | | Mesentery | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | Renal Tubule, Adenoma | X X | __________________________________________________________________________| Urethra | | __________________________________________________________________________| Urinary Bladder | + + A + + + + + + + + + + + A A + + + A + + + A +| _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X X | Lymphoma Malignant | | Lymphoma Malignant Lymphocytic | X X X X | Lymphoma Malignant Mixed | | _____________________________________________________________________________________________________________________| Page 190 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 5| 6| 7| 5| 7| 2| 4| 6| 6| 6| 6| 4| 6| 4| 6| 5| 6| 7| 6| 6| 6| 4| 5| 6| 4| | DAY ON TEST | 1| 0| 2| 1| 2| 3| 4| 5| 5| 2| 3| 8| 6| 8| 5| 1| 1| 2| 5| 9| 5| 0| 9| 6| 8| | | 3| 8| 9| 2| 9| 1| 0| 8| 9| 9| 8| 1| 7| 8| 1| 4| 1| 9| 1| 8| 0| 7| 5| 5| 1| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | 200 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE40 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + M + + + + + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Gallbladder | A + + + + A + + + + + + + A + + + + + + + + + + +| 40 | __________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + + + + A + A + + + + + + + + +| 45 | __________________________________________________________________________|____________| Intestine Large, Cecum | A + + + + + + + + + + + + A + A + + + + + + + + +| 42 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + A + A + + + + + + + + +| 44 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + A + A + + + + + + + + +| 44 | __________________________________________________________________________|____________| Intestine Small | + + + + + + + + + + + + + A + A + + + + + + + + +| 44 | __________________________________________________________________________|____________| Intestine Small, Duodenum | A + + + + + + + + + + + + A + A + + + + + + + + +| 42 | Lymphoma Malignant Mixed | X | 1 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + A + A + + + + + + + + +| 44 | Histiocytic Sarcoma | | 1 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + A + A + + + + + + + + +| 43 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Hemangioma | | 1 | Hemangiosarcoma, Metastatic, Heart | | 4 | Hepatocellular Carcinoma | X X X X X | 9 | Hepatocellular Carcinoma, Multiple | X | 5 | Hepatocellular Adenoma | X X X X X X X X| 15 | Hepatocellular Adenoma, Multiple | X X X X X X X | 12 | Histiocytic Sarcoma | X X | 4 | Lymphoma Malignant Lymphocytic | | 2 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 2 | __________________________________________________________________________|____________| Mesentery | + + + + + + +| 13 | Fat, Hemangioma | X | 2 | Fat, Hemangioma, Multiple | | 1 | Fat, Hemangiosarcoma | X | 1 | Fat, Hemangiosarcoma, Metastatic, | | | Heart | | 2 | Fat, Mast Cell Tumor NOS, Metastatic,| | | Bone Marrow | | 2 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + A + A + + + + + + + + +| 46 | Acinus, Histiocytic Sarcoma | | 2 | Acinus, Lymphoma Malignant | | | Lymphocytic | | 1 | __________________________________________________________________________|____________| Pharynx | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 191 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 5| 6| 7| 5| 7| 2| 4| 6| 6| 6| 6| 4| 6| 4| 6| 5| 6| 7| 6| 6| 6| 4| 5| 6| 4| | DAY ON TEST | 1| 0| 2| 1| 2| 3| 4| 5| 5| 2| 3| 8| 6| 8| 5| 1| 1| 2| 5| 9| 5| 0| 9| 6| 8| | | 3| 8| 9| 2| 9| 1| 0| 8| 9| 9| 8| 1| 7| 8| 1| 4| 1| 9| 1| 8| 0| 7| 5| 5| 1| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | 200 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE40 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM - cont | | | | | | Palate, Mast Cell Tumor NOS, | | | Metastatic, Bone Marrow | | 1 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + I + + + + +| 48 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + A + + + + + + + + +| 48 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + A + + + + + + + + +| 48 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | X | 3 | Squamous Cell Papilloma | | 2 | Squamous Cell Papilloma, Multiple | | 1 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + A + A + + + + + + + + +| 44 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | __________________________________________________________________________|____________| Tooth | + + + | 6 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemangiosarcoma | X X X X X X | 15 | Lymphoma Malignant Lymphocytic | X X | 4 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + A + A + + + + + + + + +| 45 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + A + A + + + + + + + + +| 45 | Adenoma | | 1 | Alveolar/Bronchiolar Carcinoma, | | | Metastatic, Lung | X | 1 | __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + A + A + + + + + + + + +| 45 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + A + A + + + + + + + + +| 46 | Adenoma | | 1 | __________________________________________________________________________|____________| Parathyroid Gland | + + + M + + + + + + M + + M + + + + + M + M + + +| 40 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + I + + + + + + + I + + + + + + + + + + +| 44 | Pars Intermedia, Adenoma | X | 1 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + A + + + + + + + + + + +| 48 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | Follicular Cell, Adenoma | X X | 2 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 192 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 5| 6| 7| 5| 7| 2| 4| 6| 6| 6| 6| 4| 6| 4| 6| 5| 6| 7| 6| 6| 6| 4| 5| 6| 4| | DAY ON TEST | 1| 0| 2| 1| 2| 3| 4| 5| 5| 2| 3| 8| 6| 8| 5| 1| 1| 2| 5| 9| 5| 0| 9| 6| 8| | | 3| 8| 9| 2| 9| 1| 0| 8| 9| 9| 8| 1| 7| 8| 1| 4| 1| 9| 1| 8| 0| 7| 5| 5| 1| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | 200 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE40 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ GENITAL SYSTEM - cont | | | | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Hemangiosarcoma, Metastatic, Heart | | 1 | __________________________________________________________________________|____________| Penis | + + | 4 | __________________________________________________________________________|____________| Preputial Gland | + + | 8 | Bilateral, Carcinoma | | 1 | __________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + A + A + + + + + + + + +| 44 | __________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + A + A + + + + + + + + +| 44 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Blood | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Histiocytic Sarcoma | X | 3 | Lymphoma Malignant Lymphocytic | X X | 4 | Mast Cell Tumor NOS | X | 3 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | Renal, Lymphoma Malignant Lymphocytic| | 3 | __________________________________________________________________________|____________| Lymph Node, Bronchial | + M + + + + + + + + M + + + + + + + + + + + + + +| 43 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X X | 5 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + M M M + + + + + + M + + M + + + + + +| 42 | Histiocytic Sarcoma | X | 2 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 2 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | M + M + M + + + + M M M + + M + + + M + + + M M +| 39 | Hemangiosarcoma, Metastatic, Heart | | 1 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X X | 6 | Lymphoma Malignant | X | 1 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 2 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + M + + M + + + M + + + + A + + + + + + + + + + +| 44 | Hemangiosarcoma, Metastatic, Heart | X | 1 | Histiocytic Sarcoma | X X | 4 | Lymphoma Malignant Lymphocytic | | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 193 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 5| 6| 7| 5| 7| 2| 4| 6| 6| 6| 6| 4| 6| 4| 6| 5| 6| 7| 6| 6| 6| 4| 5| 6| 4| | DAY ON TEST | 1| 0| 2| 1| 2| 3| 4| 5| 5| 2| 3| 8| 6| 8| 5| 1| 1| 2| 5| 9| 5| 0| 9| 6| 8| | | 3| 8| 9| 2| 9| 1| 0| 8| 9| 9| 8| 1| 7| 8| 1| 4| 1| 9| 1| 8| 0| 7| 5| 5| 1| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | 200 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE40 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Lymphoma Malignant | X | 1 | Lymphoma Malignant Mixed | X | 1 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + A + + + + + + + + +| 47 | Hemangiosarcoma, Metastatic, Heart | | 1 | Histiocytic Sarcoma | X X | 4 | Lymphoma Malignant Lymphocytic | | 3 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 2 | __________________________________________________________________________|____________| Thymus | + M + + I + + + + + + + + M + + I + + + + + + + +| 41 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X X | 5 | Lymphoma Malignant | X | 1 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 2 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M + M M M M M M M M M M M| 5 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | Subcutaneous Tissue, Hemangioma | X | 1 | Subcutaneous Tissue, Hemangiosarcoma | X | 1 | Subcutaneous Tissue, Hemangiosarcoma,| | | Metastatic, Heart | | 2 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Histiocytic Sarcoma | X | 2 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + + + + + + A + + + + + + + + + + +| 46 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 194 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 5| 6| 7| 5| 7| 2| 4| 6| 6| 6| 6| 4| 6| 4| 6| 5| 6| 7| 6| 6| 6| 4| 5| 6| 4| | DAY ON TEST | 1| 0| 2| 1| 2| 3| 4| 5| 5| 2| 3| 8| 6| 8| 5| 1| 1| 2| 5| 9| 5| 0| 9| 6| 8| | | 3| 8| 9| 2| 9| 1| 0| 8| 9| 9| 8| 1| 7| 8| 1| 4| 1| 9| 1| 8| 0| 7| 5| 5| 1| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | 200 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE40 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - cont | | | | | | Adenocarcinoma, Metastatic, | | | Harderian Gland | | 2 | Alveolar/Bronchiolar Adenoma | X X X X X X X | 16 | Alveolar/Bronchiolar Adenoma, | | | Multiple | X X X X | 8 | Alveolar/Bronchiolar Carcinoma | X X X X X X X| 13 | Alveolar/Bronchiolar Carcinoma, | | | Multiple | X X X X X X X | 9 | Hemangiosarcoma, Metastatic, Heart | | 3 | Hepatocellular Carcinoma, Metastatic,| | | Liver | X | 2 | Histiocytic Sarcoma | X X | 4 | Lymphoma Malignant Lymphocytic | X X | 6 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + A + + + + + + + + + + +| 47 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Ear | + | 1 | __________________________________________________________________________|____________| Eye | + | 1 | __________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | Adenocarcinoma | | 2 | Adenoma | X X X X X X X X X X X | 22 | Bilateral, Adenoma | X | 4 | __________________________________________________________________________|____________| Zymbal's Gland | + | 1 | Carcinoma | X | 1 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + A + + + + + + + + + + +| 48 | Hemangiosarcoma, Metastatic, Heart | | 1 | Hemangiosarcoma, Metastatic, | | | Mesentery | X | 1 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | | 2 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 195 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 5| 6| 7| 5| 7| 2| 4| 6| 6| 6| 6| 4| 6| 4| 6| 5| 6| 7| 6| 6| 6| 4| 5| 6| 4| | DAY ON TEST | 1| 0| 2| 1| 2| 3| 4| 5| 5| 2| 3| 8| 6| 8| 5| 1| 1| 2| 5| 9| 5| 0| 9| 6| 8| | | 3| 8| 9| 2| 9| 1| 0| 8| 9| 9| 8| 1| 7| 8| 1| 4| 1| 9| 1| 8| 0| 7| 5| 5| 1| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | 200 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE40 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ URINARY SYSTEM - cont | | | | | | Renal Tubule, Adenoma | X X | 4 | __________________________________________________________________________|____________| Urethra | + | 1 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + A + A + + + + + + + + +| 43 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Histiocytic Sarcoma | X X | 5 | Lymphoma Malignant | X | 1 | Lymphoma Malignant Lymphocytic | X X | 6 | Lymphoma Malignant Mixed | X | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 196 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 6| 6| 2| 4| 7| 3| 3| 5| 4| 3| 5| 2| 5| 4| 4| 3| 6| 4| 6| 4| 6| 6| 3| 3| DAY ON TEST | 4| 6| 3| 8| 2| 2| 6| 6| 5| 4| 2| 0| 1| 3| 7| 9| 8| 3| 3| 3| 4| 1| 2| 2| 1| | 9| 4| 9| 9| 5| 0| 4| 4| 4| 3| 8| 6| 7| 9| 9| 4| 2| 7| 8| 5| 1| 6| 5| 6| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 312 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE52 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + M + + + M + + + + + + + + + + + + + + + + M + +| __________________________________________________________________________| Gallbladder | A M + + A + + + + + A + A + A + A + A A + + + A A| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | A + + + + + + + + + A + A + + + + + + A + + + + A| __________________________________________________________________________| Intestine Large, Colon | A + + + + + + + + + + + A + + + + + + + + + + + A| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | A + + + + + + + + + + + A + + + + + + A + + + + A| __________________________________________________________________________| Intestine Small, Duodenum | A + + + I + + + + + + + A + + + + + + A + + + A A| __________________________________________________________________________| Intestine Small, Ileum | A + + + + + + + + + A + A + + + + + + A + + + + A| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Intestine Small, Jejunum | A + + + A + + + + + A + A + A + + + + A + + + A A| Hemangiosarcoma, Metastatic, Heart | X | Histiocytic Sarcoma | X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | X | Hemangiosarcoma | X | Hemangiosarcoma, Metastatic, Heart | X X X X X X | Hepatoblastoma | X | Hepatocellular Carcinoma | X X X X | Hepatocellular Carcinoma, Multiple | | Hepatocellular Adenoma | X X X X X | Hepatocellular Adenoma, Multiple | X X X X X X | Histiocytic Sarcoma | X X X X| Lymphoma Malignant Lymphocytic | X X | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | __________________________________________________________________________| Mesentery | + + + + + + +| Adenocarcinoma, Metastatic, Pancreas | | Fat, Hemangiosarcoma, Metastatic, | | Heart | X X X | Fat, Squamous Cell Carcinoma, | | Metastatic, Stomach | X | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Acinus, Adenocarcinoma | | Acinus, Hemangiosarcoma, Metastatic, | | Heart | | Acinus, Histiocytic Sarcoma | X | Acinus, Lymphoma Malignant | | Lymphocytic | | _____________________________________________________________________________________________________________________| Page 197 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 6| 6| 2| 4| 7| 3| 3| 5| 4| 3| 5| 2| 5| 4| 4| 3| 6| 4| 6| 4| 6| 6| 3| 3| DAY ON TEST | 4| 6| 3| 8| 2| 2| 6| 6| 5| 4| 2| 0| 1| 3| 7| 9| 8| 3| 3| 3| 4| 1| 2| 2| 1| | 9| 4| 9| 9| 5| 0| 4| 4| 4| 3| 8| 6| 7| 9| 9| 4| 2| 7| 8| 5| 1| 6| 5| 6| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 312 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE52 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM - cont | | | | Acinus, Squamous Cell Carcinoma, | | Metastatic, Stomach | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Squamous Cell Carcinoma | X | Squamous Cell Papilloma | X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + A + + + + + + + + + + + +| Squamous Cell Carcinoma, Metastatic, | | Stomach | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X X X X X X X X X X X X X X X | Histiocytic Sarcoma | X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Pancreas | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + M + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Pancreas | | Pheochromocytoma Benign | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | __________________________________________________________________________| Parathyroid Gland | M M + + + + + + + + + + M + + + + + + + + + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + + I + + + + + I + + + M + + + + + + + +| __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicular Cell, Adenoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Pancreas | | __________________________________________________________________________| Preputial Gland | + + + | Carcinoma | X X | Bilateral, Carcinoma | X | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | _____________________________________________________________________________________________________________________| Page 198 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 6| 6| 2| 4| 7| 3| 3| 5| 4| 3| 5| 2| 5| 4| 4| 3| 6| 4| 6| 4| 6| 6| 3| 3| DAY ON TEST | 4| 6| 3| 8| 2| 2| 6| 6| 5| 4| 2| 0| 1| 3| 7| 9| 8| 3| 3| 3| 4| 1| 2| 2| 1| | 9| 4| 9| 9| 5| 0| 4| 4| 4| 3| 8| 6| 7| 9| 9| 4| 2| 7| 8| 5| 1| 6| 5| 6| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 312 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE52 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X | Mast Cell Tumor NOS | X | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| Axillary, Hemangiosarcoma, | | Metastatic, Heart | | Axillary, Lymphoma Malignant | | Lymphocytic | X | Axillary, Lymphoma Malignant | X | Iliac, Lymphoma Malignant Lymphocytic| | Inguinal, Lymphoma Malignant | X | Renal, Histiocytic Sarcoma | X | Renal, Squamous Cell Carcinoma, | | Metastatic, Stomach | | __________________________________________________________________________| Lymph Node, Bronchial | + + + + M + + M + M + + M + M + M + M + + + + + +| Adenocarcinoma, Metastatic, Lung | | Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | X X X| Lymphoma Malignant Lymphocytic | X X | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + M + + M I + + M + + + + M + M M + + + + +| Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant | X | __________________________________________________________________________| Lymph Node, Mediastinal | + + + + + + + + + + M + + + M + + + + + + + + + +| Adenocarcinoma, Metastatic, Lung | | Histiocytic Sarcoma | X X X | Lymphoma Malignant Lymphocytic | X X | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + M + + + + + + M + + + + + + +| Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | X X X X X X| Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant | X | Lymphoma Malignant Mixed | X X | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + A + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | X | Histiocytic Sarcoma | X X X| Lymphoma Malignant Lymphocytic | X X | _____________________________________________________________________________________________________________________| Page 199 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 6| 6| 2| 4| 7| 3| 3| 5| 4| 3| 5| 2| 5| 4| 4| 3| 6| 4| 6| 4| 6| 6| 3| 3| DAY ON TEST | 4| 6| 3| 8| 2| 2| 6| 6| 5| 4| 2| 0| 1| 3| 7| 9| 8| 3| 3| 3| 4| 1| 2| 2| 1| | 9| 4| 9| 9| 5| 0| 4| 4| 4| 3| 8| 6| 7| 9| 9| 4| 2| 7| 8| 5| 1| 6| 5| 6| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 312 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE52 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Lymphoma Malignant Mixed | X X | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | __________________________________________________________________________| Thymus | M + M + + M + + + + + + + + + + + M I M M + M + I| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M + M M M M M M M M M M M + M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + M + + + + + + + + + + +| Sebaceous Gland, Adenoma | X | Subcutaneous Tissue, Hemangiosarcoma,| | Metastatic, Heart | X | Subcutaneous Tissue, Squamous Cell | | Carcinoma, Metastatic, Stomach | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | M | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | X | Histiocytic Sarcoma | X X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | | Adenocarcinoma, Metastatic, | | Harderian Gland | X X | Alveolar/Bronchiolar Adenoma | X X X X X | Alveolar/Bronchiolar Adenoma, | | Multiple | X X X X X | Alveolar/Bronchiolar Carcinoma | X X X | Alveolar/Bronchiolar Carcinoma, | | Multiple | X X X X | Hemangiosarcoma, Metastatic, Heart | X X X X X X X | Hemangiosarcoma, Metastatic, Liver | X | Hepatocellular Carcinoma, Metastatic,| | Liver | X | Histiocytic Sarcoma | X X X X| Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant | X | _____________________________________________________________________________________________________________________| Page 200 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 6| 6| 6| 2| 4| 7| 3| 3| 5| 4| 3| 5| 2| 5| 4| 4| 3| 6| 4| 6| 4| 6| 6| 3| 3| DAY ON TEST | 4| 6| 3| 8| 2| 2| 6| 6| 5| 4| 2| 0| 1| 3| 7| 9| 8| 3| 3| 3| 4| 1| 2| 2| 1| | 9| 4| 9| 9| 5| 0| 4| 4| 4| 3| 8| 6| 7| 9| 9| 4| 2| 7| 8| 5| 1| 6| 5| 6| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 312 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE52 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | Mediastinum, Hemangiosarcoma, | | Metastatic, Heart | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + A| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | + | __________________________________________________________________________| Eye | + + + | Adenocarcinoma, Metastatic, | | Harderian Gland | X | __________________________________________________________________________| Harderian Gland | + + + + + + + + + M + + + + + + + + + + + + + + +| Adenocarcinoma | X | Adenoma | X X X X X X X | Bilateral, Adenocarcinoma | X | Bilateral, Adenoma | X X X X X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, Pancreas | | Adenoma | | Hemangiosarcoma, Metastatic, Heart | X X X | Histiocytic Sarcoma | X X X | Lymphoma Malignant Lymphocytic | X | Renal Tubule, Adenoma | X | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + A + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X X X X X| Lymphoma Malignant | X | Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant Mixed | X X X | _____________________________________________________________________________________________________________________| Page 201 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 6| 3| 5| 4| 4| 4| 4| 5| 5| 5| 5| 7| 4| 3| 4| 5| 3| 5| 4| 5| 5| 6| 4| 6| 4| | DAY ON TEST | 0| 9| 1| 2| 2| 9| 4| 4| 5| 3| 1| 2| 4| 4| 7| 9| 6| 6| 0| 7| 4| 0| 9| 6| 4| | | 7| 2| 0| 2| 8| 5| 9| 6| 9| 9| 6| 9| 6| 4| 6| 3| 9| 3| 1| 3| 6| 4| 0| 6| 3| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | 312 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE52 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + M + + + + +| 46 | __________________________________________________________________________|____________| Gallbladder | + + M + A + A + + + A + A A + A A A A + + + A + A| 27 | __________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + A + + A + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + I + + + + + A + A A + + + + + + + + + + +| 41 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + A + A A + + + + + + + + + + +| 44 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + A + + A + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Intestine Small | + + + + + + + + + + + + A A + + + + + + + + + + +| 44 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + A + A A + M A + A + + + + + +| 38 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + A + + + A + A A + + + + + + + + A + +| 40 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X | 1 | Lymphoma Malignant Mixed | | 1 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + I + A + + + + + A A + + + + + + + + A + +| 37 | Hemangiosarcoma, Metastatic, Heart | | 1 | Histiocytic Sarcoma | | 1 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Alveolar/Bronchiolar Carcinoma, | | | Metastatic, Lung | | 1 | Hemangiosarcoma | | 1 | Hemangiosarcoma, Metastatic, Heart | X X X X X X X X X X X X| 18 | Hepatoblastoma | | 1 | Hepatocellular Carcinoma | X X X X | 8 | Hepatocellular Carcinoma, Multiple | X X | 2 | Hepatocellular Adenoma | X X X X X | 10 | Hepatocellular Adenoma, Multiple | X X X | 9 | Histiocytic Sarcoma | X | 5 | Lymphoma Malignant Lymphocytic | | 2 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | X X | 3 | __________________________________________________________________________|____________| Mesentery | + + + + + + | 13 | Adenocarcinoma, Metastatic, Pancreas | X | 1 | Fat, Hemangiosarcoma, Metastatic, | | | Heart | X X X X | 7 | Fat, Squamous Cell Carcinoma, | | | Metastatic, Stomach | X | 2 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + A + + + + + + + + + + +| 49 | Acinus, Adenocarcinoma | X | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 202 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 6| 3| 5| 4| 4| 4| 4| 5| 5| 5| 5| 7| 4| 3| 4| 5| 3| 5| 4| 5| 5| 6| 4| 6| 4| | DAY ON TEST | 0| 9| 1| 2| 2| 9| 4| 4| 5| 3| 1| 2| 4| 4| 7| 9| 6| 6| 0| 7| 4| 0| 9| 6| 4| | | 7| 2| 0| 2| 8| 5| 9| 6| 9| 9| 6| 9| 6| 4| 6| 3| 9| 3| 1| 3| 6| 4| 0| 6| 3| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | 312 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE52 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM - cont | | | | | | Acinus, Hemangiosarcoma, Metastatic, | | | Heart | X | 1 | Acinus, Histiocytic Sarcoma | | 1 | Acinus, Lymphoma Malignant | | | Lymphocytic | X | 1 | Acinus, Squamous Cell Carcinoma, | | | Metastatic, Stomach | X | 1 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + M A + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + A + + + + + + + + + M +| 48 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + A + + + + + + + + + M +| 48 | Squamous Cell Carcinoma | X X X X | 5 | Squamous Cell Papilloma | X X X | 4 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + A + + + + + + + + + M +| 47 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | X | 1 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemangiosarcoma | X X X X X X X X X X X X X X X X X X| 33 | Histiocytic Sarcoma | | 1 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + A + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + A + + + + + + + + + + +| 49 | Adenocarcinoma, Metastatic, Pancreas | X | 1 | __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + A + + + + + + + + + + +| 48 | Adenocarcinoma, Metastatic, Pancreas | X | 1 | Pheochromocytoma Benign | X | 1 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + A + + + + + + + + + + +| 49 | Adenoma | | 1 | __________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + M + + + + + M + + M + + + + + + +| 44 | __________________________________________________________________________|____________| Pituitary Gland | + + + I I + I + M + + + + + + + + + + + + + + + +| 43 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + A + + + + + + + + + + +| 49 | Follicular Cell, Adenoma | | 1 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 203 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 6| 3| 5| 4| 4| 4| 4| 5| 5| 5| 5| 7| 4| 3| 4| 5| 3| 5| 4| 5| 5| 6| 4| 6| 4| | DAY ON TEST | 0| 9| 1| 2| 2| 9| 4| 4| 5| 3| 1| 2| 4| 4| 7| 9| 6| 6| 0| 7| 4| 0| 9| 6| 4| | | 7| 2| 0| 2| 8| 5| 9| 6| 9| 9| 6| 9| 6| 4| 6| 3| 9| 3| 1| 3| 6| 4| 0| 6| 3| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | 312 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE52 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ GENITAL SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Adenocarcinoma, Metastatic, Pancreas | X | 1 | __________________________________________________________________________|____________| Preputial Gland | + | 4 | Carcinoma | X | 3 | Bilateral, Carcinoma | | 1 | __________________________________________________________________________|____________| Prostate | + + + + M + + + + + + + + A + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Seminal Vesicle | + + + + + + A + + + + + + A + + + + + + + + A + +| 47 | __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Blood | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + A + + + + + + + + + + +| 49 | Histiocytic Sarcoma | | 2 | Lymphoma Malignant Lymphocytic | | 2 | Mast Cell Tumor NOS | | 1 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Axillary, Hemangiosarcoma, | | | Metastatic, Heart | X | 1 | Axillary, Lymphoma Malignant | | | Lymphocytic | | 1 | Axillary, Lymphoma Malignant | | 1 | Iliac, Lymphoma Malignant Lymphocytic| X | 1 | Inguinal, Lymphoma Malignant | | 1 | Renal, Histiocytic Sarcoma | | 1 | Renal, Squamous Cell Carcinoma, | | | Metastatic, Stomach | X | 1 | __________________________________________________________________________|____________| Lymph Node, Bronchial | + + + M + + M M + + + + M + + + M + M + + + + + +| 37 | Adenocarcinoma, Metastatic, Lung | X | 1 | Hemangiosarcoma, Metastatic, Heart | X | 1 | Histiocytic Sarcoma | | 3 | Lymphoma Malignant Lymphocytic | X | 3 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | | 1 | __________________________________________________________________________|____________| Lymph Node, Mandibular | M + + M + + M M M + M + M + + + M M M + + + + M M| 31 | Lymphoma Malignant Lymphocytic | | 2 | Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | + + M + + + + + + + + + M M + M M M + + + M + + +| 41 | Adenocarcinoma, Metastatic, Lung | X | 1 | Histiocytic Sarcoma | X | 4 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 204 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 6| 3| 5| 4| 4| 4| 4| 5| 5| 5| 5| 7| 4| 3| 4| 5| 3| 5| 4| 5| 5| 6| 4| 6| 4| | DAY ON TEST | 0| 9| 1| 2| 2| 9| 4| 4| 5| 3| 1| 2| 4| 4| 7| 9| 6| 6| 0| 7| 4| 0| 9| 6| 4| | | 7| 2| 0| 2| 8| 5| 9| 6| 9| 9| 6| 9| 6| 4| 6| 3| 9| 3| 1| 3| 6| 4| 0| 6| 3| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | 312 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE52 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Lymphoma Malignant Lymphocytic | | 2 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | X | 2 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + M + + + + + + + + M + + + + I + + + M + +| 44 | Hemangiosarcoma, Metastatic, Heart | X| 1 | Histiocytic Sarcoma | | 6 | Lymphoma Malignant Lymphocytic | X X | 4 | Lymphoma Malignant | | 1 | Lymphoma Malignant Mixed | | 2 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | X X | 3 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + A + + + + + + + + + + +| 48 | Hemangiosarcoma, Metastatic, Heart | X X | 3 | Histiocytic Sarcoma | X | 4 | Lymphoma Malignant Lymphocytic | X | 3 | Lymphoma Malignant Mixed | | 2 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | __________________________________________________________________________|____________| Thymus | + M + + + + + + + + + M M + + + + + + + + + + M +| 37 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | | 2 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M + M M M M M M M M M M M M I M M + M M M M M M I| 4 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Sebaceous Gland, Adenoma | X | 2 | Subcutaneous Tissue, Hemangiosarcoma,| | | Metastatic, Heart | X X X | 4 | Subcutaneous Tissue, Squamous Cell | | | Carcinoma, Metastatic, Stomach | X | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Skeletal Muscle | | | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemangiosarcoma, Metastatic, Heart | X X X X | 5 | Histiocytic Sarcoma | | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 205 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 6| 3| 5| 4| 4| 4| 4| 5| 5| 5| 5| 7| 4| 3| 4| 5| 3| 5| 4| 5| 5| 6| 4| 6| 4| | DAY ON TEST | 0| 9| 1| 2| 2| 9| 4| 4| 5| 3| 1| 2| 4| 4| 7| 9| 6| 6| 0| 7| 4| 0| 9| 6| 4| | | 7| 2| 0| 2| 8| 5| 9| 6| 9| 9| 6| 9| 6| 4| 6| 3| 9| 3| 1| 3| 6| 4| 0| 6| 3| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | 312 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE52 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ NERVOUS SYSTEM - cont | | | | | | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + + + + + + A + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Adenocarcinoma | X | 1 | Adenocarcinoma, Metastatic, | | | Harderian Gland | | 2 | Alveolar/Bronchiolar Adenoma | X X X X X X X X X| 14 | Alveolar/Bronchiolar Adenoma, | | | Multiple | X X X X X X X | 12 | Alveolar/Bronchiolar Carcinoma | X X X X X X | 9 | Alveolar/Bronchiolar Carcinoma, | | | Multiple | X X | 6 | Hemangiosarcoma, Metastatic, Heart | X X X X X X X X X X X| 18 | Hemangiosarcoma, Metastatic, Liver | | 1 | Hepatocellular Carcinoma, Metastatic,| | | Liver | | 1 | Histiocytic Sarcoma | X | 5 | Lymphoma Malignant Lymphocytic | | 1 | Lymphoma Malignant | | 1 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | | 1 | Mediastinum, Hemangiosarcoma, | | | Metastatic, Heart | X | 1 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + A + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + A A + + + + + + + + + + +| 47 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Ear | | 1 | __________________________________________________________________________|____________| Eye | + | 4 | Adenocarcinoma, Metastatic, | | | Harderian Gland | | 1 | __________________________________________________________________________|____________| Harderian Gland | + + + + I + + + + + + + + + + + + + + + + + + + +| 48 | Adenocarcinoma | | 1 | Adenoma | X X X X X X X X X X X X X| 20 | Bilateral, Adenocarcinoma | | 1 | Bilateral, Adenoma | X X X | 8 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 206 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 6| 3| 5| 4| 4| 4| 4| 5| 5| 5| 5| 7| 4| 3| 4| 5| 3| 5| 4| 5| 5| 6| 4| 6| 4| | DAY ON TEST | 0| 9| 1| 2| 2| 9| 4| 4| 5| 3| 1| 2| 4| 4| 7| 9| 6| 6| 0| 7| 4| 0| 9| 6| 4| | | 7| 2| 0| 2| 8| 5| 9| 6| 9| 9| 6| 9| 6| 4| 6| 3| 9| 3| 1| 3| 6| 4| 0| 6| 3| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | 312 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE52 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ URINARY SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + A + + + + + + + + + + +| 49 | Adenocarcinoma, Metastatic, Pancreas | X | 1 | Adenoma | X | 1 | Hemangiosarcoma, Metastatic, Heart | X X X | 6 | Histiocytic Sarcoma | X | 4 | Lymphoma Malignant Lymphocytic | | 1 | Renal Tubule, Adenoma | X | 2 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + A + + + + + + A + + + + + + + + + + +| 47 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Histiocytic Sarcoma | X | 7 | Lymphoma Malignant | | 1 | Lymphoma Malignant Lymphocytic | X X | 4 | Lymphoma Malignant Mixed | | 3 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 207 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 7| 5| 6| 5| 4| 1| 5| 7| 1| 7| 3| 5| 6| 5| 5| 5| 2| 6| 6| 6| 3| 7| 4| 3| DAY ON TEST | 3| 2| 8| 7| 6| 1| 8| 6| 0| 9| 2| 4| 0| 9| 3| 9| 6| 8| 5| 2| 3| 1| 2| 7| 0| | 1| 9| 0| 7| 7| 0| 5| 6| 7| 4| 9| 2| 6| 2| 9| 5| 7| 5| 0| 3| 8| 2| 9| 1| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 625 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE13 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + M + M + + + + + + + + M +| __________________________________________________________________________| Gallbladder | + + + + + + A A + A + + + A + + + A A + + A + A A| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + A + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + A A + + + + + + + + + A + + + A + A A| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + A + + + + + + + + + + + + + A + + A| __________________________________________________________________________| Intestine Large, Rectum | M + + + + + A + + + + + + + + + + + + + + A + + +| __________________________________________________________________________| Intestine Small | + + + + + + A A + A + + + + + + + + + + + A + + A| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + A A + A + + + + + + + A + + + M + + A| Lymphoma Malignant Lymphocytic | X | Lymphoma Malignant Mixed | X | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + A A + A + + + + + + + A + + + A + A A| Lymphoma Malignant Mixed | X | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + A A + A + + + + + + + + + + + A + + A| Carcinoma | X | Histiocytic Sarcoma | X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + A + + +| Hemangiosarcoma | X | Hemangiosarcoma, Metastatic, Heart | X | Hepatocellular Carcinoma | X X X | Hepatocellular Carcinoma, Multiple | X X X X X X | Hepatocellular Adenoma | X X X X X X X X X | Hepatocellular Adenoma, Multiple | X X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X| Lymphoma Malignant Mixed | | __________________________________________________________________________| Mesentery | + + + + + + +| Fat, Hemangioma | X | Fat, Hemangiosarcoma | X | Fat, Lymphoma Malignant Lymphocytic | X| Fat, Squamous Cell Carcinoma, | | Metastatic, Stomach | | __________________________________________________________________________| Pancreas | + + + + + + A + + + + + + + + + + + + + + A + + +| Acinus, Histiocytic Sarcoma | | Acinus, Lymphoma Malignant | | Lymphocytic | X X | Acinus, Lymphoma Malignant Mixed | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | Squamous Cell Carcinoma | X X | Squamous Cell Papilloma | X X | _____________________________________________________________________________________________________________________| Page 208 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 7| 5| 6| 5| 4| 1| 5| 7| 1| 7| 3| 5| 6| 5| 5| 5| 2| 6| 6| 6| 3| 7| 4| 3| DAY ON TEST | 3| 2| 8| 7| 6| 1| 8| 6| 0| 9| 2| 4| 0| 9| 3| 9| 6| 8| 5| 2| 3| 1| 2| 7| 0| | 1| 9| 0| 7| 7| 0| 5| 6| 7| 4| 9| 2| 6| 2| 9| 5| 7| 5| 0| 3| 8| 2| 9| 1| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 625 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE13 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM - cont | | | | Squamous Cell Papilloma, Multiple | X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + A + + + + + + + + + + + + + A + A +| Lymphoma Malignant Lymphocytic | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Tooth | + + + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | X | Hemangiosarcoma | X X X X X X | Lymphoma Malignant Lymphocytic | X X X X| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | Lymphoma Malignant Mixed | | Bilateral, Adenoma | X | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Pheochromocytoma Benign | X X | __________________________________________________________________________| Islets, Pancreatic | + + + + + + A + + + + + + + + + + + + + + A + + +| __________________________________________________________________________| Parathyroid Gland | M + + + + M M + M M + M M M + + M + M + + + + + +| __________________________________________________________________________| Pituitary Gland | + + + I + + + + M + + + + + + + + + I + + + + M +| Lymphoma Malignant Lymphocytic | X| __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicular Cell, Adenoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | __________________________________________________________________________| Tissue NOS | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Preputial Gland | + + + + | Adenoma | X | Carcinoma | | Bilateral, Carcinoma | | __________________________________________________________________________| Prostate | + + + + + + + I + + + + + + + + + A + + + + + + +| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Seminal Vesicle | + + + + + + A A + + + + + + + + + A + + + A + + +| __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangioma | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | _____________________________________________________________________________________________________________________| Page 209 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 7| 5| 6| 5| 4| 1| 5| 7| 1| 7| 3| 5| 6| 5| 5| 5| 2| 6| 6| 6| 3| 7| 4| 3| DAY ON TEST | 3| 2| 8| 7| 6| 1| 8| 6| 0| 9| 2| 4| 0| 9| 3| 9| 6| 8| 5| 2| 3| 1| 2| 7| 0| | 1| 9| 0| 7| 7| 0| 5| 6| 7| 4| 9| 2| 6| 2| 9| 5| 7| 5| 0| 3| 8| 2| 9| 1| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 625 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE13 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + A + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X X X| Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + A + + +| Axillary, Lymphoma Malignant | | Lymphocytic | X X X| Axillary, Lymphoma Malignant Mixed | | Deep Cervical, Lymphoma Malignant | | Lymphocytic | | Iliac, Lymphoma Malignant Lymphocytic| X| Inguinal, Lymphoma Malignant | | Lymphocytic | X X| Renal, Histiocytic Sarcoma | | Renal, Lymphoma Malignant Lymphocytic| X X| Renal, Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node, Bronchial | + + + M M + + + M + + + + M + + + + + + + A + + +| Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X| Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + M M + + + M M + + + M M + M A + + +| Lymphoma Malignant Lymphocytic | X X X X X| Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node, Mediastinal | + M + + + M + M + + + M + + + + + + + + + A + + +| Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | X | Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X| Lymphoma Malignant Mixed | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + A + + M + + + + + + M I + M + A + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X| Lymphoma Malignant Mixed | X | Squamous Cell Carcinoma, Metastatic, | | Stomach | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + A + + +| Hemangiosarcoma | | Histiocytic Sarcoma | X | _____________________________________________________________________________________________________________________| Page 210 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 7| 5| 6| 5| 4| 1| 5| 7| 1| 7| 3| 5| 6| 5| 5| 5| 2| 6| 6| 6| 3| 7| 4| 3| DAY ON TEST | 3| 2| 8| 7| 6| 1| 8| 6| 0| 9| 2| 4| 0| 9| 3| 9| 6| 8| 5| 2| 3| 1| 2| 7| 0| | 1| 9| 0| 7| 7| 0| 5| 6| 7| 4| 9| 2| 6| 2| 9| 5| 7| 5| 0| 3| 8| 2| 9| 1| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 625 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE13 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Lymphoma Malignant Lymphocytic | X X X X X X| Lymphoma Malignant Mixed | | __________________________________________________________________________| Thymus | + + + + + + + + + + M + + M + + + + I + M A I + +| Histiocytic Sarcoma | | Lymphoma Malignant Lymphocytic | X X X X X X X| Lymphoma Malignant Mixed | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M + M M M M M M M M M M M + + M M M +| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Sebaceous Gland, Adenoma | | Subcutaneous Tissue, Hemangiosarcoma | X | Subcutaneous Tissue, Lymphoma | | Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Neuroblastoma Malignant, Metastatic, | | Brain | X | __________________________________________________________________________| Skeletal Muscle | | Lymphoma Malignant Lymphocytic | | Abdominal, Squamous Cell Carcinoma, | | Metastatic, Stomach | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Metastatic, | | Harderian Gland | X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X| Olfactory Lobe, Glioma Malignant | | Olfactory Lobe, Neuroblastoma | | Malignant | X X | __________________________________________________________________________| Spinal Cord | + | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma, Multiple | | Adenocarcinoma, Metastatic, | | Harderian Gland | X X X | Alveolar/Bronchiolar Adenoma | X X X X | Alveolar/Bronchiolar Adenoma, | | _____________________________________________________________________________________________________________________| Page 211 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 7| 5| 6| 5| 4| 1| 5| 7| 1| 7| 3| 5| 6| 5| 5| 5| 2| 6| 6| 6| 3| 7| 4| 3| DAY ON TEST | 3| 2| 8| 7| 6| 1| 8| 6| 0| 9| 2| 4| 0| 9| 3| 9| 6| 8| 5| 2| 3| 1| 2| 7| 0| | 1| 9| 0| 7| 7| 0| 5| 6| 7| 4| 9| 2| 6| 2| 9| 5| 7| 5| 0| 3| 8| 2| 9| 1| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 625 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE13 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Multiple | X X X X | Alveolar/Bronchiolar Carcinoma | X X X X X X X | Alveolar/Bronchiolar Carcinoma, | | Multiple | X X X | Carcinoma, Metastatic, Preputial | | Gland | | Carcinoma, Metastatic, Zymbal's Gland| X | Hemangiosarcoma, Metastatic, Heart | | Hemangiosarcoma, Metastatic, Liver | X | Hepatocellular Carcinoma, Metastatic,| | Liver | X X X X X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X| Lymphoma Malignant Mixed | | Squamous Cell Carcinoma, Metastatic, | | Stomach | | Mediastinum, Adenocarcinoma, | | Metastatic, Lung | | Mediastinum, Alveolar/Bronchiolar | | Carcinoma, Metastatic, Lung | X | Mediastinum, Histiocytic Sarcoma | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | Neuroblastoma Malignant, Metastatic, | | Brain | X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + A + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | + | __________________________________________________________________________| Eye | + M + + + | __________________________________________________________________________| Harderian Gland | I + + + + + M + + M + + + + + + + + + + + + + + +| Adenocarcinoma | X X X X | Adenoma | X X X X X X X X | Bilateral, Adenoma | X X X | __________________________________________________________________________| Zymbal's Gland | + + | Carcinoma | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X| Lymphoma Malignant Mixed | | Squamous Cell Carcinoma, Metastatic, | | _____________________________________________________________________________________________________________________| Page 212 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 7| 5| 6| 5| 4| 1| 5| 7| 1| 7| 3| 5| 6| 5| 5| 5| 2| 6| 6| 6| 3| 7| 4| 3| DAY ON TEST | 3| 2| 8| 7| 6| 1| 8| 6| 0| 9| 2| 4| 0| 9| 3| 9| 6| 8| 5| 2| 3| 1| 2| 7| 0| | 1| 9| 0| 7| 7| 0| 5| 6| 7| 4| 9| 2| 6| 2| 9| 5| 7| 5| 0| 3| 8| 2| 9| 1| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 625 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE13 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| URINARY SYSTEM - cont | | | | Stomach | | Renal Tubule, Adenoma | X | __________________________________________________________________________| Urinary Bladder | + + + + + + + A + + + + + + + + + + + + + A + + +| Hemangioma | X | Lymphoma Malignant Lymphocytic | X| _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X X X| Lymphoma Malignant Mixed | X | _____________________________________________________________________________________________________________________| Page 213 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 5| 2| 3| 6| 6| 2| 3| 6| 6| 7| 2| 2| 7| 5| 2| 6| 5| 5| 2| 1| 7| 2| 6| 3| 2| | DAY ON TEST | 2| 4| 2| 6| 3| 7| 5| 1| 3| 2| 2| 9| 2| 5| 7| 9| 5| 9| 8| 6| 2| 5| 1| 7| 0| | | 0| 7| 7| 6| 1| 0| 2| 7| 8| 9| 4| 6| 9| 8| 1| 3| 3| 5| 4| 9| 3| 1| 1| 0| 8| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| T | ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| A | 625 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE13 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 47 | __________________________________________________________________________|____________| Gallbladder | + + + + + A + A A + A A + + + + + + A A + + + A A| 32 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Intestine Large | + + + + + + + + A + + + + + + + + + + + + + + A A| 46 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + A + A A + + + + + + + + + + + A A| 39 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + A + + + + + + + + + + + + + + A A| 44 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + A + + + + + + + + + + + + + + A A| 44 | __________________________________________________________________________|____________| Intestine Small | + + + + + A + + A + + A + + + + + + + + + + + A +| 41 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + A + + A + A A + + + + M + A M + + + A A| 35 | Lymphoma Malignant Lymphocytic | X | 2 | Lymphoma Malignant Mixed | X | 2 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + A + + A + A A + + + + + + A + + + + A +| 37 | Lymphoma Malignant Mixed | X | 2 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + A + + A + + A + + + + A + + + + + + A +| 40 | Carcinoma | | 1 | Histiocytic Sarcoma | | 1 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Hemangiosarcoma | X | 2 | Hemangiosarcoma, Metastatic, Heart | X | 2 | Hepatocellular Carcinoma | X X X X | 7 | Hepatocellular Carcinoma, Multiple | X | 7 | Hepatocellular Adenoma | X X X X X | 14 | Hepatocellular Adenoma, Multiple | X X X | 5 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X X X X X | 11 | Lymphoma Malignant Mixed | X X | 2 | __________________________________________________________________________|____________| Mesentery | + + + + | 11 | Fat, Hemangioma | X | 2 | Fat, Hemangiosarcoma | | 1 | Fat, Lymphoma Malignant Lymphocytic | | 1 | Fat, Squamous Cell Carcinoma, | | | Metastatic, Stomach | X X | 2 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + A + + + + + + + + + + + + + + A +| 46 | Acinus, Histiocytic Sarcoma | X | 1 | Acinus, Lymphoma Malignant | | | Lymphocytic | X X X| 5 | Acinus, Lymphoma Malignant Mixed | X | 1 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant Lymphocytic | X | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 214 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 5| 2| 3| 6| 6| 2| 3| 6| 6| 7| 2| 2| 7| 5| 2| 6| 5| 5| 2| 1| 7| 2| 6| 3| 2| | DAY ON TEST | 2| 4| 2| 6| 3| 7| 5| 1| 3| 2| 2| 9| 2| 5| 7| 9| 5| 9| 8| 6| 2| 5| 1| 7| 0| | | 0| 7| 7| 6| 1| 0| 2| 7| 8| 9| 4| 6| 9| 8| 1| 3| 3| 5| 4| 9| 3| 1| 1| 0| 8| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| T | ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| A | 625 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE13 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant Lymphocytic | X | 1 | Squamous Cell Carcinoma | X X | 4 | Squamous Cell Papilloma | X | 3 | Squamous Cell Papilloma, Multiple | | 1 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + A + + A + A + + + + + + + + + + + + + +| 44 | Lymphoma Malignant Lymphocytic | X | 1 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | X | 1 | __________________________________________________________________________|____________| Tooth | + | 4 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Alveolar/Bronchiolar Carcinoma, | | | Metastatic, Lung | | 1 | Hemangiosarcoma | X | 7 | Lymphoma Malignant Lymphocytic | X X X X | 8 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant Lymphocytic | X X | 2 | Lymphoma Malignant Mixed | X | 1 | Bilateral, Adenoma | | 1 | __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + + M| 49 | Pheochromocytoma Benign | | 2 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + A + + + + + + + + + + + + + + M M| 45 | __________________________________________________________________________|____________| Parathyroid Gland | + M + + + + + M + + + + + + + M M + + M + + M + M| 33 | __________________________________________________________________________|____________| Pituitary Gland | + I + + + + + + + + + + + + I + + + + + M + + + +| 43 | Lymphoma Malignant Lymphocytic | X X | 3 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Follicular Cell, Adenoma | X X | 2 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | __________________________________________________________________________|____________| Tissue NOS | + | 1 | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + I + + + + + + + + + + + + + +| 49 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 215 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 5| 2| 3| 6| 6| 2| 3| 6| 6| 7| 2| 2| 7| 5| 2| 6| 5| 5| 2| 1| 7| 2| 6| 3| 2| | DAY ON TEST | 2| 4| 2| 6| 3| 7| 5| 1| 3| 2| 2| 9| 2| 5| 7| 9| 5| 9| 8| 6| 2| 5| 1| 7| 0| | | 0| 7| 7| 6| 1| 0| 2| 7| 8| 9| 4| 6| 9| 8| 1| 3| 3| 5| 4| 9| 3| 1| 1| 0| 8| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| T | ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| A | 625 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE13 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ GENITAL SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Preputial Gland | + + + + + + | 10 | Adenoma | | 1 | Carcinoma | X X X | 3 | Bilateral, Carcinoma | X | 1 | __________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Seminal Vesicle | + + + + + A + + A + + + + + + + + + + + + M + A +| 42 | __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemangioma | X | 1 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Blood | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + A + + + + + + + + + + + + + + + +| 48 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X X X X X X X X X| 17 | Lymphoma Malignant Mixed | X | 1 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Axillary, Lymphoma Malignant | | | Lymphocytic | X X X| 6 | Axillary, Lymphoma Malignant Mixed | X | 1 | Deep Cervical, Lymphoma Malignant | | | Lymphocytic | X | 1 | Iliac, Lymphoma Malignant Lymphocytic| X X| 3 | Inguinal, Lymphoma Malignant | | | Lymphocytic | X| 3 | Renal, Histiocytic Sarcoma | X | 1 | Renal, Lymphoma Malignant Lymphocytic| X| 3 | Renal, Lymphoma Malignant Mixed | X | 1 | __________________________________________________________________________|____________| Lymph Node, Bronchial | + + M + + + + + + + + M + + + + + M + + M + M + +| 40 | Alveolar/Bronchiolar Carcinoma, | | | Metastatic, Lung | | 1 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant Lymphocytic | X X X X X X| 13 | Lymphoma Malignant Mixed | X | 1 | __________________________________________________________________________|____________| Lymph Node, Mandibular | M + + + + I + + + + M + + + + + + + + + + + + + +| 39 | Lymphoma Malignant Lymphocytic | X X X X X X| 11 | Lymphoma Malignant Mixed | X X | 2 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | M + M M + + + + M + + + M + + + + + + + + + I + +| 39 | Alveolar/Bronchiolar Carcinoma, | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 216 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 5| 2| 3| 6| 6| 2| 3| 6| 6| 7| 2| 2| 7| 5| 2| 6| 5| 5| 2| 1| 7| 2| 6| 3| 2| | DAY ON TEST | 2| 4| 2| 6| 3| 7| 5| 1| 3| 2| 2| 9| 2| 5| 7| 9| 5| 9| 8| 6| 2| 5| 1| 7| 0| | | 0| 7| 7| 6| 1| 0| 2| 7| 8| 9| 4| 6| 9| 8| 1| 3| 3| 5| 4| 9| 3| 1| 1| 0| 8| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| T | ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| A | 625 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE13 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Metastatic, Lung | | 1 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant Lymphocytic | X X X X X X X| 13 | Lymphoma Malignant Mixed | X X X | 3 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | X X | 2 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + M + + + + + + + + + + + + + + + +| 43 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X X X X X X X| 12 | Lymphoma Malignant Mixed | X X X | 4 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | X | 1 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Hemangiosarcoma | X | 1 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X X X X X X X| 13 | Lymphoma Malignant Mixed | X X | 2 | __________________________________________________________________________|____________| Thymus | + + + M I + M + A + + + M + + + + + + + M + + M +| 37 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant Lymphocytic | X X X X X X X X| 15 | Lymphoma Malignant Mixed | X X | 2 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M + M M M M M M M M M M M M M M M| 5 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Sebaceous Gland, Adenoma | X X | 2 | Subcutaneous Tissue, Hemangiosarcoma | | 1 | Subcutaneous Tissue, Lymphoma | | | Malignant Lymphocytic | X | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Neuroblastoma Malignant, Metastatic, | | | Brain | | 1 | __________________________________________________________________________|____________| Skeletal Muscle | + + | 2 | Lymphoma Malignant Lymphocytic | X | 1 | Abdominal, Squamous Cell Carcinoma, | | | Metastatic, Stomach | X | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 217 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 5| 2| 3| 6| 6| 2| 3| 6| 6| 7| 2| 2| 7| 5| 2| 6| 5| 5| 2| 1| 7| 2| 6| 3| 2| | DAY ON TEST | 2| 4| 2| 6| 3| 7| 5| 1| 3| 2| 2| 9| 2| 5| 7| 9| 5| 9| 8| 6| 2| 5| 1| 7| 0| | | 0| 7| 7| 6| 1| 0| 2| 7| 8| 9| 4| 6| 9| 8| 1| 3| 3| 5| 4| 9| 3| 1| 1| 0| 8| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| T | ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| A | 625 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE13 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ NERVOUS SYSTEM - cont | | | | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Adenocarcinoma, Metastatic, | | | Harderian Gland | | 1 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X X X | 4 | Olfactory Lobe, Glioma Malignant | X X | 2 | Olfactory Lobe, Neuroblastoma | | | Malignant | | 2 | __________________________________________________________________________|____________| Spinal Cord | | 1 | Lymphoma Malignant Lymphocytic | | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + A + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Adenocarcinoma, Multiple | X | 1 | Adenocarcinoma, Metastatic, | | | Harderian Gland | | 3 | Alveolar/Bronchiolar Adenoma | X X X X X X X | 11 | Alveolar/Bronchiolar Adenoma, | | | Multiple | X X | 6 | Alveolar/Bronchiolar Carcinoma | X X X X X | 12 | Alveolar/Bronchiolar Carcinoma, | | | Multiple | X X | 5 | Carcinoma, Metastatic, Preputial | | | Gland | X | 1 | Carcinoma, Metastatic, Zymbal's Gland| | 1 | Hemangiosarcoma, Metastatic, Heart | X | 1 | Hemangiosarcoma, Metastatic, Liver | | 1 | Hepatocellular Carcinoma, Metastatic,| | | Liver | | 5 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X X X X X | 9 | Lymphoma Malignant Mixed | X | 1 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | X | 1 | Mediastinum, Adenocarcinoma, | | | Metastatic, Lung | X | 1 | Mediastinum, Alveolar/Bronchiolar | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 218 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 5| 2| 3| 6| 6| 2| 3| 6| 6| 7| 2| 2| 7| 5| 2| 6| 5| 5| 2| 1| 7| 2| 6| 3| 2| | DAY ON TEST | 2| 4| 2| 6| 3| 7| 5| 1| 3| 2| 2| 9| 2| 5| 7| 9| 5| 9| 8| 6| 2| 5| 1| 7| 0| | | 0| 7| 7| 6| 1| 0| 2| 7| 8| 9| 4| 6| 9| 8| 1| 3| 3| 5| 4| 9| 3| 1| 1| 0| 8| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| T | ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| A | 625 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE13 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - cont | | | | | | Carcinoma, Metastatic, Lung | | 1 | Mediastinum, Histiocytic Sarcoma | X | 1 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant Lymphocytic | X X X | 3 | Neuroblastoma Malignant, Metastatic, | | | Brain | | 1 | __________________________________________________________________________|____________| Trachea | + + + + + + + + A + + + + + + + + + + + + + + + +| 48 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Ear | | 1 | __________________________________________________________________________|____________| Eye | | 4 | __________________________________________________________________________|____________| Harderian Gland | + M + + + + + + + + I + + + + + + + + M + M + + M| 42 | Adenocarcinoma | | 4 | Adenoma | X X X X X | 13 | Bilateral, Adenoma | X X X X | 7 | __________________________________________________________________________|____________| Zymbal's Gland | + | 3 | Carcinoma | X | 2 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemangiosarcoma, Metastatic, Heart | X | 1 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X X X X | 7 | Lymphoma Malignant Mixed | X | 1 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | X | 1 | Renal Tubule, Adenoma | | 1 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + A + + + + + + + + + + + A +| 46 | Hemangioma | | 1 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant Lymphocytic | X X X X X X X X X| 17 | Lymphoma Malignant Mixed | X X X X | 5 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 219 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 2| 3| 4| 3| 5| 4| 2| 2| 2| 3| 3| 3| 4| 4| 3| 2| 5| 5| 5| 2| 2| 2| 3| 2| DAY ON TEST | 8| 0| 0| 9| 1| 5| 8| 6| 8| 3| 5| 1| 5| 1| 2| 6| 4| 1| 4| 5| 1| 7| 2| 0| 6| | 8| 3| 1| 2| 3| 4| 3| 8| 8| 1| 4| 3| 9| 2| 9| 4| 5| 3| 3| 3| 5| 0| 2| 6| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 625 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE 26 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + M + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + A A A A + + + + + + M + A M A| Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + + A + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + A A + + + + + + A + A + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + A + A + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + M + + + + + + + + + A + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + A + + + + + + A + + + +| __________________________________________________________________________| Intestine Small, Duodenum | A + + + A + + + + + A A A A + + + + A + A + + A A| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + A + A + + + + A + A + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Intestine Small, Jejunum | A + + + + + + + + + A + + A + + + + + + A + + M +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X | Hemangiosarcoma, Metastatic, Heart | X X | Hepatocellular Carcinoma | X | Hepatocellular Carcinoma, Multiple | | Hepatocellular Adenoma | X X | Hepatocellular Adenoma, Multiple | X | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X | Lymphoma Malignant Mixed | | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | __________________________________________________________________________| Mesentery | + + + + | Fat, Histiocytic Sarcoma | X | Fat, Squamous Cell Carcinoma, | | Metastatic, Stomach | X | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + A + +| Acinus, Hemangiosarcoma, Metastatic, | | Liver | X | Acinus, Histiocytic Sarcoma | X | Acinus, Lymphoma Malignant | | Lymphocytic | X X X X X | Acinus, Squamous Cell Carcinoma, | | Metastatic, Stomach | X | __________________________________________________________________________| Pharynx | + + | _____________________________________________________________________________________________________________________| Page 220 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 2| 3| 4| 3| 5| 4| 2| 2| 2| 3| 3| 3| 4| 4| 3| 2| 5| 5| 5| 2| 2| 2| 3| 2| DAY ON TEST | 8| 0| 0| 9| 1| 5| 8| 6| 8| 3| 5| 1| 5| 1| 2| 6| 4| 1| 4| 5| 1| 7| 2| 0| 6| | 8| 3| 1| 2| 3| 4| 3| 8| 8| 1| 4| 3| 9| 2| 9| 4| 5| 3| 3| 3| 5| 0| 2| 6| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 625 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE 26 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM - cont | | | | Carcinoma, Metastatic, Zymbal's Gland| X | Squamous Cell Carcinoma | X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X X X | __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | Squamous Cell Carcinoma | X X X X | Squamous Cell Papilloma | X X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Zymbal's Gland| X | Hemangiosarcoma | X X X X X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X| Lymphoma Malignant Mixed | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant Mixed | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + A + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + A + +| __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + + M + I I + M + + M + + + + +| __________________________________________________________________________| Pituitary Gland | + + I + + + + + + + + + I + + M M + + + + + + + +| Carcinoma, Metastatic, Zymbal's Gland| X | Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | _____________________________________________________________________________________________________________________| Page 221 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 2| 3| 4| 3| 5| 4| 2| 2| 2| 3| 3| 3| 4| 4| 3| 2| 5| 5| 5| 2| 2| 2| 3| 2| DAY ON TEST | 8| 0| 0| 9| 1| 5| 8| 6| 8| 3| 5| 1| 5| 1| 2| 6| 4| 1| 4| 5| 1| 7| 2| 0| 6| | 8| 3| 1| 2| 3| 4| 3| 8| 8| 1| 4| 3| 9| 2| 9| 4| 5| 3| 3| 3| 5| 0| 2| 6| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 625 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE 26 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| GENITAL SYSTEM - cont | | | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X | Lymphoma Malignant Mixed | | __________________________________________________________________________| Preputial Gland | + | Carcinoma | | Bilateral, Carcinoma | | __________________________________________________________________________| Prostate | + + + + + + + + + + M + + + + + + + + M + + M + +| Lymphoma Malignant Lymphocytic | X X | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + A + A + +| Hemangiosarcoma, Metastatic, Liver | X | Lymphoma Malignant Lymphocytic | | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X X| Lymphoma Malignant Mixed | | Mast Cell Tumor NOS | X | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | Axillary, Lymphoma Malignant | | Lymphocytic | X X X | Axillary, Lymphoma Malignant Mixed | | Iliac, Lymphoma Malignant Lymphocytic| X X | Inguinal, Lymphoma Malignant | | Lymphocytic | X X | Inguinal, Squamous Cell Carcinoma, | | Metastatic, Stomach | X | Pancreatic, Lymphoma Malignant | | Lymphocytic | X | Renal, Lymphoma Malignant Lymphocytic| X X X | __________________________________________________________________________| Lymph Node, Bronchial | + + + M + + + + + + + + + + M + + M + + + I + + +| Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | X | Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X| Lymphoma Malignant Mixed | | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + M M M + + + + + M + + + + + +| Histiocytic Sarcoma | X X | _____________________________________________________________________________________________________________________| Page 222 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 2| 3| 4| 3| 5| 4| 2| 2| 2| 3| 3| 3| 4| 4| 3| 2| 5| 5| 5| 2| 2| 2| 3| 2| DAY ON TEST | 8| 0| 0| 9| 1| 5| 8| 6| 8| 3| 5| 1| 5| 1| 2| 6| 4| 1| 4| 5| 1| 7| 2| 0| 6| | 8| 3| 1| 2| 3| 4| 3| 8| 8| 1| 4| 3| 9| 2| 9| 4| 5| 3| 3| 3| 5| 0| 2| 6| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 625 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE 26 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X | Lymphoma Malignant Mixed | | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | Squamous Cell Carcinoma, Metastatic, | | Pharynx | X | __________________________________________________________________________| Lymph Node, Mediastinal | + + + M + + + + + + + + + + + + + + M + + + + + +| Alveolar/Bronchiolar Carcinoma, | | Metastatic, Lung | X | Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X X| Lymphoma Malignant Mixed | | Squamous Cell Carcinoma, Metastatic, | | Stomach | X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + M + + + + + + + + + + + M + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X | Lymphoma Malignant Mixed | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X X | Lymphoma Malignant Mixed | | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | Osteosarcoma, Metastatic, Uncertain | | Primary Site | | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | __________________________________________________________________________| Thymus | + + + M + M + + + + + M M + I + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X X| Lymphoma Malignant Mixed | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M + M M M M M + M M M M + M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X | Subcutaneous Tissue, Leiomyosarcoma | | Subcutaneous Tissue, Lymphoma | | Malignant Mixed | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | + + | Diaphragm, Hemangiosarcoma, | | _____________________________________________________________________________________________________________________| Page 223 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 2| 3| 4| 3| 5| 4| 2| 2| 2| 3| 3| 3| 4| 4| 3| 2| 5| 5| 5| 2| 2| 2| 3| 2| DAY ON TEST | 8| 0| 0| 9| 1| 5| 8| 6| 8| 3| 5| 1| 5| 1| 2| 6| 4| 1| 4| 5| 1| 7| 2| 0| 6| | 8| 3| 1| 2| 3| 4| 3| 8| 8| 1| 4| 3| 9| 2| 9| 4| 5| 3| 3| 3| 5| 0| 2| 6| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 625 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE 26 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM - cont | | | | Metastatic, Liver | X | Diaphragm, Squamous Cell Carcinoma, | | Metastatic, Stomach | X | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant Lymphocytic | X X X X X X X X | Olfactory Lobe, Glioma Malignant | | __________________________________________________________________________| Spinal Cord | + | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X X X | Alveolar/Bronchiolar Adenoma, | | Multiple | X X X | Alveolar/Bronchiolar Carcinoma | X X X | Alveolar/Bronchiolar Carcinoma, | | Multiple | X X X | Carcinoma, Metastatic, Zymbal's Gland| X | Hemangiosarcoma, Metastatic, Heart | X | Hemangiosarcoma, Metastatic, Liver | X | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X| Lymphoma Malignant Mixed | | Osteosarcoma, Metastatic, Uncertain | | Primary Site | | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | Mediastinum, Alveolar/Bronchiolar | | Carcinoma, Metastatic, Lung | X | Mediastinum, Carcinoma, Metastatic, | | Zymbal's Gland | X | Mediastinum, Hemangiosarcoma, | | Metastatic, Heart | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant Lymphocytic | X X X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + A + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + | __________________________________________________________________________| Harderian Gland | + M + + I + + M + + + + + + + + + + + + + M M + M| _____________________________________________________________________________________________________________________| Page 224 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 _____________________________________________________________________________________________________________________ | 2| 2| 3| 4| 3| 5| 4| 2| 2| 2| 3| 3| 3| 4| 4| 3| 2| 5| 5| 5| 2| 2| 2| 3| 2| DAY ON TEST | 8| 0| 0| 9| 1| 5| 8| 6| 8| 3| 5| 1| 5| 1| 2| 6| 4| 1| 4| 5| 1| 7| 2| 0| 6| | 8| 3| 1| 2| 3| 4| 3| 8| 8| 1| 4| 3| 9| 2| 9| 4| 5| 3| 3| 3| 5| 0| 2| 6| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 625 PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| SE 26 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM - cont | | | | Adenoma | X X X X X X | Bilateral, Adenoma | | __________________________________________________________________________| Zymbal's Gland | + | Carcinoma | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma, Metastatic, Heart | | Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X | Lymphoma Malignant Mixed | | Mast Cell Tumor NOS, Metastatic, | | Bone Marrow | X | Osteosarcoma, Metastatic, Uncertain | | Primary Site | | Squamous Cell Carcinoma, Metastatic, | | Stomach | X | Renal Tubule, Adenoma | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + I + +| Lymphoma Malignant Lymphocytic | X X X X X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X X X| Lymphoma Malignant Mixed | | _____________________________________________________________________________________________________________________| Page 225 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 6| 1| 5| 5| 2| 2| 2| 5| 3| 2| 1| 2| 2| 5| 4| 4| 4| 1| 2| 5| 3| 4| 5| 2| 2| | DAY ON TEST | 3| 5| 1| 4| 3| 4| 5| 5| 5| 0| 9| 4| 6| 3| 4| 1| 8| 7| 5| 0| 5| 4| 6| 2| 5| | | 5| 9| 0| 4| 5| 1| 1| 8| 8| 9| 4| 0| 8| 9| 0| 4| 3| 8| 0| 2| 6| 1| 2| 3| 5| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| T | ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| A | 625 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE 26 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + M + + + + +| 48 | __________________________________________________________________________|____________| Gallbladder | + + A + A A + + A + + + A + + + A A + A A + + + A| 32 | Lymphoma Malignant Lymphocytic | X X | 3 | __________________________________________________________________________|____________| Intestine Large | + + + + A + + + A + + + + + + + + + + + + + + + +| 47 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + A A + + A + + + + + + + + A + + + + + + +| 42 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X | 2 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + A A + + A + + + + + + + + + + + + + + + +| 45 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + A + + + A + + + + + + + + + + + + + + + +| 46 | __________________________________________________________________________|____________| Intestine Small | + + + + A + + + A + + + + + + + + + + + + + + + +| 46 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + A A + + A + + + A M + + A + + A A + + + +| 32 | __________________________________________________________________________|____________| Intestine Small, Ileum | + A + + A A + + A + + + A + + + + A + + + + + + +| 40 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X | 2 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + A + + + A + + + + + + + A A + + + + + + +| 41 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemangiosarcoma | | 1 | Hemangiosarcoma, Metastatic, Heart | X X X | 5 | Hepatocellular Carcinoma | X X | 3 | Hepatocellular Carcinoma, Multiple | X | 1 | Hepatocellular Adenoma | X X X X X | 7 | Hepatocellular Adenoma, Multiple | X X X | 4 | Histiocytic Sarcoma | | 2 | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X X| 25 | Lymphoma Malignant Mixed | X X | 2 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | | 1 | __________________________________________________________________________|____________| Mesentery | | 4 | Fat, Histiocytic Sarcoma | | 1 | Fat, Squamous Cell Carcinoma, | | | Metastatic, Stomach | | 1 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Acinus, Hemangiosarcoma, Metastatic, | | | Liver | | 1 | Acinus, Histiocytic Sarcoma | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 226 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 6| 1| 5| 5| 2| 2| 2| 5| 3| 2| 1| 2| 2| 5| 4| 4| 4| 1| 2| 5| 3| 4| 5| 2| 2| | DAY ON TEST | 3| 5| 1| 4| 3| 4| 5| 5| 5| 0| 9| 4| 6| 3| 4| 1| 8| 7| 5| 0| 5| 4| 6| 2| 5| | | 5| 9| 0| 4| 5| 1| 1| 8| 8| 9| 4| 0| 8| 9| 0| 4| 3| 8| 0| 2| 6| 1| 2| 3| 5| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| T | ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| A | 625 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE 26 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM - cont | | | | | | Acinus, Lymphoma Malignant | | | Lymphocytic | X X X X X X X X | 13 | Acinus, Squamous Cell Carcinoma, | | | Metastatic, Stomach | | 1 | __________________________________________________________________________|____________| Pharynx | | 2 | Carcinoma, Metastatic, Zymbal's Gland| | 1 | Squamous Cell Carcinoma | | 1 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant Lymphocytic | X X X X X X | 10 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant Lymphocytic | X | 2 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | Squamous Cell Carcinoma | X X | 6 | Squamous Cell Papilloma | X X | 4 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + A + + + + + + A + + + + A + + + + + + +| 47 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X X X X X | 7 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | | 1 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Carcinoma, Metastatic, Zymbal's Gland| | 1 | Hemangiosarcoma | X X X X X X X X | 13 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X X X X X X X| 12 | Lymphoma Malignant Mixed | X | 1 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant Lymphocytic | X X | 4 | Lymphoma Malignant Mixed | X | 1 | __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + A + + + + + + + + + + + + + + + + + + + +| 48 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + I + + + +| 48 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 227 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 6| 1| 5| 5| 2| 2| 2| 5| 3| 2| 1| 2| 2| 5| 4| 4| 4| 1| 2| 5| 3| 4| 5| 2| 2| | DAY ON TEST | 3| 5| 1| 4| 3| 4| 5| 5| 5| 0| 9| 4| 6| 3| 4| 1| 8| 7| 5| 0| 5| 4| 6| 2| 5| | | 5| 9| 0| 4| 5| 1| 1| 8| 8| 9| 4| 0| 8| 9| 0| 4| 3| 8| 0| 2| 6| 1| 2| 3| 5| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| T | ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| A | 625 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE 26 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Parathyroid Gland | + M M + + + M M + M + + M + + + + + M M + + + + +| 37 | __________________________________________________________________________|____________| Pituitary Gland | M + + + + + + + I + + + + + + + + + M + + + + + +| 43 | Carcinoma, Metastatic, Zymbal's Gland| | 1 | Lymphoma Malignant Lymphocytic | X X| 4 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + A + + + + + + + + + + + + + + + +| 49 | Lymphoma Malignant Lymphocytic | X X X | 3 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant Lymphocytic | X | 3 | Lymphoma Malignant Mixed | X | 1 | __________________________________________________________________________|____________| Preputial Gland | + + + + + | 6 | Carcinoma | X X | 2 | Bilateral, Carcinoma | X | 1 | __________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| 47 | Lymphoma Malignant Lymphocytic | X X X| 5 | __________________________________________________________________________|____________| Seminal Vesicle | + + + + A + + + + + + + + + + + + + + + + + + + +| 47 | Hemangiosarcoma, Metastatic, Liver | | 1 | Lymphoma Malignant Lymphocytic | X X| 2 | __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant Lymphocytic | | 1 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Blood | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + A + + + + + + + + + + + + + + + +| 49 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X| 27 | Lymphoma Malignant Mixed | X X | 2 | Mast Cell Tumor NOS | | 1 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant Lymphocytic | | 1 | Axillary, Lymphoma Malignant | | | Lymphocytic | X X X X | 7 | Axillary, Lymphoma Malignant Mixed | X | 1 | Iliac, Lymphoma Malignant Lymphocytic| X X | 4 | Inguinal, Lymphoma Malignant | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 228 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 6| 1| 5| 5| 2| 2| 2| 5| 3| 2| 1| 2| 2| 5| 4| 4| 4| 1| 2| 5| 3| 4| 5| 2| 2| | DAY ON TEST | 3| 5| 1| 4| 3| 4| 5| 5| 5| 0| 9| 4| 6| 3| 4| 1| 8| 7| 5| 0| 5| 4| 6| 2| 5| | | 5| 9| 0| 4| 5| 1| 1| 8| 8| 9| 4| 0| 8| 9| 0| 4| 3| 8| 0| 2| 6| 1| 2| 3| 5| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| T | ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| A | 625 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE 26 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Lymphocytic | X X | 4 | Inguinal, Squamous Cell Carcinoma, | | | Metastatic, Stomach | | 1 | Pancreatic, Lymphoma Malignant | | | Lymphocytic | | 1 | Renal, Lymphoma Malignant Lymphocytic| X X X X | 7 | __________________________________________________________________________|____________| Lymph Node, Bronchial | M + + M + + + + + + + + + M + M + + + + M + + + +| 41 | Alveolar/Bronchiolar Carcinoma, | | | Metastatic, Lung | | 1 | Hemangiosarcoma, Metastatic, Heart | X | 1 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X| 26 | Lymphoma Malignant Mixed | X X | 2 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + I + + + + + + + + + + + + M + + M + + + +| 43 | Histiocytic Sarcoma | | 2 | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X| 24 | Lymphoma Malignant Mixed | X X | 2 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | Squamous Cell Carcinoma, Metastatic, | | | Pharynx | | 1 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | M + + + M + + + + + + + + + + + + + + + M M + + +| 44 | Alveolar/Bronchiolar Carcinoma, | | | Metastatic, Lung | | 1 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X| 27 | Lymphoma Malignant Mixed | X X | 2 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | | 2 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X X| 26 | Lymphoma Malignant Mixed | X X X | 3 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Histiocytic Sarcoma | | 2 | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X| 27 | Lymphoma Malignant Mixed | X X | 2 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 229 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 6| 1| 5| 5| 2| 2| 2| 5| 3| 2| 1| 2| 2| 5| 4| 4| 4| 1| 2| 5| 3| 4| 5| 2| 2| | DAY ON TEST | 3| 5| 1| 4| 3| 4| 5| 5| 5| 0| 9| 4| 6| 3| 4| 1| 8| 7| 5| 0| 5| 4| 6| 2| 5| | | 5| 9| 0| 4| 5| 1| 1| 8| 8| 9| 4| 0| 8| 9| 0| 4| 3| 8| 0| 2| 6| 1| 2| 3| 5| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| T | ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| A | 625 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE 26 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Osteosarcoma, Metastatic, Uncertain | | | Primary Site | X | 1 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | | 1 | __________________________________________________________________________|____________| Thymus | M + + + + + + + M + + + + M M + + + + + + M + + +| 40 | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X| 26 | Lymphoma Malignant Mixed | X | 1 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M M M M M + M M M + M M + M M I M + + M M M M + M| 9 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant Lymphocytic | | 1 | Subcutaneous Tissue, Leiomyosarcoma | X | 1 | Subcutaneous Tissue, Lymphoma | | | Malignant Mixed | X | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Skeletal Muscle | | 2 | Diaphragm, Hemangiosarcoma, | | | Metastatic, Liver | | 1 | Diaphragm, Squamous Cell Carcinoma, | | | Metastatic, Stomach | | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant Lymphocytic | X X X X X| 13 | Olfactory Lobe, Glioma Malignant | X | 1 | __________________________________________________________________________|____________| Spinal Cord | + | 2 | Lymphoma Malignant Lymphocytic | X | 2 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | I + + + + + + + A + + + + + + + + + + + + + + + +| 47 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Alveolar/Bronchiolar Adenoma | X X X X | 7 | Alveolar/Bronchiolar Adenoma, | | | Multiple | X X | 5 | Alveolar/Bronchiolar Carcinoma | X X X | 6 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 230 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 6| 1| 5| 5| 2| 2| 2| 5| 3| 2| 1| 2| 2| 5| 4| 4| 4| 1| 2| 5| 3| 4| 5| 2| 2| | DAY ON TEST | 3| 5| 1| 4| 3| 4| 5| 5| 5| 0| 9| 4| 6| 3| 4| 1| 8| 7| 5| 0| 5| 4| 6| 2| 5| | | 5| 9| 0| 4| 5| 1| 1| 8| 8| 9| 4| 0| 8| 9| 0| 4| 3| 8| 0| 2| 6| 1| 2| 3| 5| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| T | ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| A | 625 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE 26 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - cont | | | | | | Alveolar/Bronchiolar Carcinoma, | | | Multiple | X X | 5 | Carcinoma, Metastatic, Zymbal's Gland| | 1 | Hemangiosarcoma, Metastatic, Heart | X X | 3 | Hemangiosarcoma, Metastatic, Liver | | 1 | Histiocytic Sarcoma | | 2 | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X| 23 | Lymphoma Malignant Mixed | X X | 2 | Osteosarcoma, Metastatic, Uncertain | | | Primary Site | X | 1 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | | 1 | Mediastinum, Alveolar/Bronchiolar | | | Carcinoma, Metastatic, Lung | | 1 | Mediastinum, Carcinoma, Metastatic, | | | Zymbal's Gland | | 1 | Mediastinum, Hemangiosarcoma, | | | Metastatic, Heart | X | 1 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant Lymphocytic | X X X| 6 | __________________________________________________________________________|____________| Trachea | + + + + + + + + A + + + + + + + + + + + + + + + +| 48 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | + + | 3 | __________________________________________________________________________|____________| Harderian Gland | + M + + M M + + + M M M + + + + + + M + + + + + M| 36 | Adenoma | X X X X | 10 | Bilateral, Adenoma | X X X | 3 | __________________________________________________________________________|____________| Zymbal's Gland | + | 2 | Carcinoma | X | 2 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemangiosarcoma, Metastatic, Heart | X | 1 | Histiocytic Sarcoma | | 2 | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X| 23 | Lymphoma Malignant Mixed | X X | 2 | Mast Cell Tumor NOS, Metastatic, | | | Bone Marrow | | 1 | Osteosarcoma, Metastatic, Uncertain | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 231 NTP Experiment-Test: 05134-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC 1,3-BUTADIENE Date: 04/02/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 08:02:08 __________________________________________________________________________________________________________________________________ | 6| 1| 5| 5| 2| 2| 2| 5| 3| 2| 1| 2| 2| 5| 4| 4| 4| 1| 2| 5| 3| 4| 5| 2| 2| | DAY ON TEST | 3| 5| 1| 4| 3| 4| 5| 5| 5| 0| 9| 4| 6| 3| 4| 1| 8| 7| 5| 0| 5| 4| 6| 2| 5| | | 5| 9| 0| 4| 5| 1| 1| 8| 8| 9| 4| 0| 8| 9| 0| 4| 3| 8| 0| 2| 6| 1| 2| 3| 5| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| T | ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| A | 625 PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| L | SE 26 | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | __________________________________________________________________________________________________________________________________ URINARY SYSTEM - cont | | | | | | Primary Site | X | 1 | Squamous Cell Carcinoma, Metastatic, | | | Stomach | | 1 | Renal Tubule, Adenoma | X | 1 | __________________________________________________________________________|____________| Urinary Bladder | + + + + A + + + + + + + + + + + + + + + + + + + +| 48 | Lymphoma Malignant Lymphocytic | X X X X X X X X| 13 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Histiocytic Sarcoma | | 2 | Lymphoma Malignant Lymphocytic | X X X X X X X X X X X X X X X| 30 | Lymphoma Malignant Mixed | X X X | 3 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 232 ------------------------------------------------------------ ---------- END OF REPORT 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