TDMS Study 05150-02 Pathology Tables
NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 Facility: TSI Mason Research Chemical CAS #: 298-59-9 Lock Date: 02/19/92 Cage Range: All Reasons For Removal: All Removal Date Range: All Treatment Groups: Include All Page 1 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 4| 4| 7| 2| 0| 6| 7| 7| 7| 7| 4| 4| 7| 7| 6| 7| 4| 7| 4| 2| 7| 7| 2| 4| 7| DAY ON TEST | 5| 5| 4| 8| 6| 2| 4| 4| 4| 4| 5| 5| 4| 4| 7| 4| 5| 4| 5| 8| 4| 4| 8| 5| 4| | 6| 5| 7| 1| 7| 8| 6| 7| 7| 7| 5| 5| 7| 6| 3| 7| 5| 7| 6| 0| 7| 7| 0| 6| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 0 PPM | 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + M + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + A + + + + + + + + A + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + A + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + M + + + A + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | 2 3 3 4 2 3 3 2 2 2 4| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + A + + + + M + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + A + + + + + + + + A + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + A + + + + + + + + A + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Autolysis | 3 | Basophilic Focus | X X | Congestion | 2 | Eosinophilic Focus | | Fatty Change | | Hematopoietic Cell Proliferation | | Hyperplasia, Lymphoid | | Necrosis, Focal | 2 1 1 | __________________________________________________________________________| Mesentery | +| Fat, Necrosis | 3| __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2 | Duct, Ectasia | 4 | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2 2 2 | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Abscess | | Diverticulum | | Ulcer | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Muscularis, Hypertrophy | | __________________________________________________________________________| Tongue | + | Angiectasis | 2 | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + M + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | 1 | Hematopoietic Cell Proliferation | | Hyperplasia, Focal | | Capsule, Hyperplasia | 2 | _____________________________________________________________________________________________________________________| Page 2 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 4| 4| 7| 2| 0| 6| 7| 7| 7| 7| 4| 4| 7| 7| 6| 7| 4| 7| 4| 2| 7| 7| 2| 4| 7| DAY ON TEST | 5| 5| 4| 8| 6| 2| 4| 4| 4| 4| 5| 5| 4| 4| 7| 4| 5| 4| 5| 8| 4| 4| 8| 5| 4| | 6| 5| 7| 1| 7| 8| 6| 7| 7| 7| 5| 5| 7| 6| 3| 7| 5| 7| 6| 0| 7| 7| 0| 6| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 0 PPM | 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Adrenal Medulla | M + + M + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 3 3 1 | __________________________________________________________________________| Parathyroid Gland | M M + M M + + M + + M M M M + + M + + M + + M + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + I + + + + + + + +| Pars Distalis, Angiectasis | | Pars Distalis, Hyperplasia, Focal | 2 4 2 2 2| __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicular Cell, Hyperplasia, Focal | 1 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | __________________________________________________________________________| Tissue NOS | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | M + + + + + + + + + + + M +| Cyst | | Dilatation | 3 3 | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + M + + + + M + + + + + +| Cyst | X X X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 3 | Hydrometra | 4 2 | Inflammation, Suppurative | | Endometrium, Hyperplasia, Cystic | 2 2 4 3 1 4 1 2 4 2 3 4 3 4 2 1 3 3 4 4 3 2 | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Neutrophil | | Myelofibrosis | 2 3 1 2 1 1 4 2 | __________________________________________________________________________| Lymph Node | + | Lumbar, Hematopoietic Cell | | Proliferation | | Mediastinal, Hyperplasia, Lymphoid | | Pancreatic, Hyperplasia, Lymphoid | | Renal, Hematopoietic Cell | | Proliferation | | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + M + + + + + + + +| Hematopoietic Cell Proliferation | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + M + + + + + + + + + + + + + + + + + + +| Congestion | 4 | Depletion Lymphoid | 4 | Inflammation, Chronic | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 3 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 4| 4| 7| 2| 0| 6| 7| 7| 7| 7| 4| 4| 7| 7| 6| 7| 4| 7| 4| 2| 7| 7| 2| 4| 7| DAY ON TEST | 5| 5| 4| 8| 6| 2| 4| 4| 4| 4| 5| 5| 4| 4| 7| 4| 5| 4| 5| 8| 4| 4| 8| 5| 4| | 6| 5| 7| 1| 7| 8| 6| 7| 7| 7| 5| 5| 7| 6| 3| 7| 5| 7| 6| 0| 7| 7| 0| 6| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 0 PPM | 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Congestion | 2 | Depletion Lymphoid | 2 | Hematopoietic Cell Proliferation | 3 2 3 | Hyperplasia, Lymphoid | | __________________________________________________________________________| Thymus | + + + + + + + + + + + M + M M + + + + + + + + + +| Depletion Lymphoid | 4 | Hyperplasia, Lymphoid | 3 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + M + M + + + + + + M + + + + + + + + +| Lactation | | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Ulcer | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Compression | | Mineralization, Focal | 1 | Mineralization | 2 1 2 2 1 1 2 1 2 1 2 2 2 2 2| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + M + + + + + + + + + + +| Congestion | 2 | Hemorrhage, Focal | | Inflammation, Chronic | 3 | Peribronchial, Inflammation, Chronic | 2 | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Degeneration, Hyaline | 2 1 2 | Inflammation, Chronic, Focal | 2 2 2 | Inflammation, Chronic | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | + | __________________________________________________________________________| Harderian Gland | + | Hyperplasia | 2 | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Fatty Change | 2 2 | _____________________________________________________________________________________________________________________| Page 4 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 4| 4| 7| 2| 0| 6| 7| 7| 7| 7| 4| 4| 7| 7| 6| 7| 4| 7| 4| 2| 7| 7| 2| 4| 7| DAY ON TEST | 5| 5| 4| 8| 6| 2| 4| 4| 4| 4| 5| 5| 4| 4| 7| 4| 5| 4| 5| 8| 4| 4| 8| 5| 4| | 6| 5| 7| 1| 7| 8| 6| 7| 7| 7| 5| 5| 7| 6| 3| 7| 5| 7| 6| 0| 7| 7| 0| 6| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 0 PPM | 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| URINARY SYSTEM - cont | | | | Glomerulosclerosis | 3 | Hemorrhage, Focal | 3 | Hyperplasia, Lymphoid | | Infarct | | Mineralization | 4 | Renal Tubule, Degeneration, Granular | | Renal Tubule, Regeneration | 2 1 | __________________________________________________________________________| Urinary Bladder | + + + + + A + + + + + + + + A + + + + + + + + + +| Inflammation, Chronic, Focal | 2 2 2 2 1 2 | _____________________________________________________________________________________________________________________| Page 5 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 2| 7| 7| 5| 7| 3| 2| 7| 7| 7| 5| 7| 2| 7| 7| 7| 7| 7| 7| 6| 7| 6| 7| 7| 7| DAY ON TEST | 8| 4| 4| 7| 4| 4| 8| 4| 4| 4| 8| 4| 8| 0| 4| 0| 4| 4| 0| 2| 4| 8| 4| 4| 4| | 1| 7| 7| 1| 7| 8| 0| 7| 7| 7| 1| 7| 0| 8| 7| 8| 7| 7| 8| 1| 7| 5| 7| 7| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 0 PPM | 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 6| 7| 8| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + A + + + + M + + + + + + + + + + + + A + + +| __________________________________________________________________________| Intestine Large, Colon | + + + A + + + + + + + + + + + + + + + + + A + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + A + + + + + + + + + + + + + + + + + A + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + A + + + + + + + + + + + + + + + + + A + + +| Hyperplasia, Lymphoid | 4 4 3 2 3 3 3 3 4 2 2 | __________________________________________________________________________| Intestine Small, Duodenum | + + + A + + + + + + + + + + + + + + + + + A + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + A + + + + + + + + + + + + + + + + + A + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + A + + + + + + + + + + + + + + + + + A + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 1 | Autolysis | | Basophilic Focus | | Congestion | | Eosinophilic Focus | | Fatty Change | | Hematopoietic Cell Proliferation | 2 | Hyperplasia, Lymphoid | 2 | Necrosis, Focal | 2 1 | __________________________________________________________________________| Mesentery | + | Fat, Necrosis | 3 | __________________________________________________________________________| Pancreas | + + + A + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 1 | Duct, Ectasia | 4 | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | | __________________________________________________________________________| Stomach, Forestomach | + + + A + + + + + + + + + + + + + + + + + + + + +| Abscess | | Diverticulum | | Ulcer | 4 | __________________________________________________________________________| Stomach, Glandular | + + + A + + + + + + + + + + + + + + + + + + + + +| Muscularis, Hypertrophy | 3 | __________________________________________________________________________| Tongue | + + | Angiectasis | 3 3 | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | | Hematopoietic Cell Proliferation | 3 | Hyperplasia, Focal | 1 | Capsule, Hyperplasia | | _____________________________________________________________________________________________________________________| Page 6 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 2| 7| 7| 5| 7| 3| 2| 7| 7| 7| 5| 7| 2| 7| 7| 7| 7| 7| 7| 6| 7| 6| 7| 7| 7| DAY ON TEST | 8| 4| 4| 7| 4| 4| 8| 4| 4| 4| 8| 4| 8| 0| 4| 0| 4| 4| 0| 2| 4| 8| 4| 4| 4| | 1| 7| 7| 1| 7| 8| 0| 7| 7| 7| 1| 7| 0| 8| 7| 8| 7| 7| 8| 1| 7| 5| 7| 7| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 0 PPM | 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 6| 7| 8| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | | __________________________________________________________________________| Islets, Pancreatic | + + + A + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 1 3 | __________________________________________________________________________| Parathyroid Gland | + + + M + + M + M + M M I + + + + + + + M + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + M + + +| Pars Distalis, Angiectasis | 1 | Pars Distalis, Hyperplasia, Focal | 4 1 2 2 2 3 2| __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicular Cell, Hyperplasia, Focal | 1 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | __________________________________________________________________________| Tissue NOS | + | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | + + + + + + + + + M + + + M M + + + + + +| Cyst | | Dilatation | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + M M + + + + + +| Cyst | X X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Hydrometra | 4 4 | Inflammation, Suppurative | 4 | Endometrium, Hyperplasia, Cystic | 3 3 3 3 4 1 1 2 3 4 1 3 3 4 3 3 4 1 3 3 2 3| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Neutrophil | 4 | Myelofibrosis | 2 2 1 1 1 1 3 1 2 4 1 1 1| __________________________________________________________________________| Lymph Node | + + + | Lumbar, Hematopoietic Cell | | Proliferation | 4 | Mediastinal, Hyperplasia, Lymphoid | | Pancreatic, Hyperplasia, Lymphoid | 4 | Renal, Hematopoietic Cell | | Proliferation | 4 | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + I + + + + +| Hematopoietic Cell Proliferation | 4 | __________________________________________________________________________| Lymph Node, Mesenteric | + + + M + + + + + + + + + + + + M + + + + M + + +| Congestion | | Depletion Lymphoid | | Inflammation, Chronic | 4 | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + M + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 7 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 2| 7| 7| 5| 7| 3| 2| 7| 7| 7| 5| 7| 2| 7| 7| 7| 7| 7| 7| 6| 7| 6| 7| 7| 7| DAY ON TEST | 8| 4| 4| 7| 4| 4| 8| 4| 4| 4| 8| 4| 8| 0| 4| 0| 4| 4| 0| 2| 4| 8| 4| 4| 4| | 1| 7| 7| 1| 7| 8| 0| 7| 7| 7| 1| 7| 0| 8| 7| 8| 7| 7| 8| 1| 7| 5| 7| 7| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 0 PPM | 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 6| 7| 8| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Congestion | | Depletion Lymphoid | | Hematopoietic Cell Proliferation | 4 1 4 | Hyperplasia, Lymphoid | 3 | __________________________________________________________________________| Thymus | M + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Lymphoid | | Hyperplasia, Lymphoid | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + M + + + + + + + + + + + + + + + + M + + + +| Lactation | X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Ulcer | 3 | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | + | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Compression | 4 | Mineralization, Focal | | Mineralization | 2 2 3 1 2 2 2 1 2 1 2 2 2 2 3| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | | Hemorrhage, Focal | 4 | Inflammation, Chronic | | Peribronchial, Inflammation, Chronic | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Degeneration, Hyaline | 2 2 | Inflammation, Chronic, Focal | 1 2 2 | Inflammation, Chronic | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | | __________________________________________________________________________| Harderian Gland | | Hyperplasia | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Fatty Change | | _____________________________________________________________________________________________________________________| Page 8 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 2| 7| 7| 5| 7| 3| 2| 7| 7| 7| 5| 7| 2| 7| 7| 7| 7| 7| 7| 6| 7| 6| 7| 7| 7| DAY ON TEST | 8| 4| 4| 7| 4| 4| 8| 4| 4| 4| 8| 4| 8| 0| 4| 0| 4| 4| 0| 2| 4| 8| 4| 4| 4| | 1| 7| 7| 1| 7| 8| 0| 7| 7| 7| 1| 7| 0| 8| 7| 8| 7| 7| 8| 1| 7| 5| 7| 7| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 0 PPM | 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 6| 7| 8| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| _____________________________________________________________________________________________________________________| URINARY SYSTEM - cont | | | | Glomerulosclerosis | 3 | Hemorrhage, Focal | | Hyperplasia, Lymphoid | | Infarct | | Mineralization | | Renal Tubule, Degeneration, Granular | 3 | Renal Tubule, Regeneration | 2 1 | __________________________________________________________________________| Urinary Bladder | + + + A + + + + + + + + + + + + + + + + M A + + +| Inflammation, Chronic, Focal | 1 | _____________________________________________________________________________________________________________________| Page 9 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 4| 7| 4| 7| 2| 4| 2| 2| 7| 7| 2| 6| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 5| 4| 5| 4| 8| 5| 8| 8| 4| 4| 8| 8| 4| 4| 4| 4| 4| 4| 4| | | | 6| 7| 6| 7| 0| 5| 1| 1| 7| 7| 0| 4| 7| 7| 7| 7| 7| 7| 7| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 0 PPM | 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| | L | | 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + + + + | 64 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + | 66 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + | 65 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + | 67 | Hyperplasia, Lymphoid | 2 2 2 2 3 3 2 4 | 30 2.8| __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + | 65 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + | 65 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + | 65 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + | 69 | Angiectasis | | 1 1.0| Autolysis | | 1 3.0| Basophilic Focus | | 2 | Congestion | | 1 2.0| Eosinophilic Focus | X X X | 3 | Fatty Change | 2 | 1 2.0| Hematopoietic Cell Proliferation | | 1 2.0| Hyperplasia, Lymphoid | 2 | 2 2.0| Necrosis, Focal | 1 1 | 7 1.3| __________________________________________________________________________|____________| Mesentery | + + + + | 6 | Fat, Necrosis | 3 3 2 | 5 2.8| __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + | 68 | Inflammation, Chronic, Focal | | 2 1.5| Duct, Ectasia | | 2 4.0| __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + | 69 | Inflammation, Chronic, Focal | 2 3 2 | 6 2.2| __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + M + + + + + + + | 67 | Abscess | 3 | 1 3.0| Diverticulum | 3 | 1 3.0| Ulcer | | 1 4.0| __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + | 68 | Muscularis, Hypertrophy | | 1 3.0| __________________________________________________________________________|____________| Tongue | | 3 | Angiectasis | | 3 2.7| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + | 68 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 10 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 4| 7| 4| 7| 2| 4| 2| 2| 7| 7| 2| 6| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 5| 4| 5| 4| 8| 5| 8| 8| 4| 4| 8| 8| 4| 4| 4| 4| 4| 4| 4| | | | 6| 7| 6| 7| 0| 5| 1| 1| 7| 7| 0| 4| 7| 7| 7| 7| 7| 7| 7| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 0 PPM | 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| | L | | 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + | 69 | Congestion | | 1 1.0| Hematopoietic Cell Proliferation | | 1 3.0| Hyperplasia, Focal | | 1 1.0| Capsule, Hyperplasia | | 1 2.0| __________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + | 67 | Hyperplasia, Focal | 2 | 1 2.0| __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + | 68 | Hyperplasia | 2 4 | 7 2.4| __________________________________________________________________________|____________| Parathyroid Gland | M + + + M M M M + + M M + + + + + + + | 43 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + | 67 | Pars Distalis, Angiectasis | | 1 1.0| Pars Distalis, Hyperplasia, Focal | 3 4 | 14 2.5| __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + | 69 | Follicular Cell, Hyperplasia, Focal | 2 1 | 4 1.3| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | __________________________________________________________________________|____________| Tissue NOS | + | 2 | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Clitoral Gland | + + + + + + + + + + + + + | 43 | Cyst | X | 1 | Dilatation | | 2 3.0| __________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + | 65 | Cyst | X X | 7 | __________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + | 69 | Angiectasis | | 1 3.0| Hydrometra | 2 4 | 6 3.3| Inflammation, Suppurative | | 1 4.0| Endometrium, Hyperplasia, Cystic | 1 1 1 1 2 1 1 1 3 1 3 1 2 3 3 3 2 2 4 | 63 2.5| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + | 69 | Hyperplasia, Neutrophil | 2 | 2 3.0| Myelofibrosis | 1 2 2 2 | 25 1.8| __________________________________________________________________________|____________| Lymph Node | + + + | 7 | Lumbar, Hematopoietic Cell | | | Proliferation | | 1 4.0| Mediastinal, Hyperplasia, Lymphoid | 4 | 1 4.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 11 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 4| 7| 4| 7| 2| 4| 2| 2| 7| 7| 2| 6| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 5| 4| 5| 4| 8| 5| 8| 8| 4| 4| 8| 8| 4| 4| 4| 4| 4| 4| 4| | | | 6| 7| 6| 7| 0| 5| 1| 1| 7| 7| 0| 4| 7| 7| 7| 7| 7| 7| 7| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 0 PPM | 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| | L | | 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Pancreatic, Hyperplasia, Lymphoid | | 1 4.0| Renal, Hematopoietic Cell | | | Proliferation | | 1 4.0| __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + | 67 | Hematopoietic Cell Proliferation | | 1 4.0| __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + | 65 | Congestion | | 1 4.0| Depletion Lymphoid | | 1 4.0| Inflammation, Chronic | | 1 4.0| __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + | 68 | Congestion | | 1 2.0| Depletion Lymphoid | | 1 2.0| Hematopoietic Cell Proliferation | 3 4 | 8 3.0| Hyperplasia, Lymphoid | 2 | 2 2.5| __________________________________________________________________________|____________| Thymus | + + + + + + + + + + + + + + + + + + + | 65 | Depletion Lymphoid | | 1 4.0| Hyperplasia, Lymphoid | 4 | 2 3.5| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | + + + M + + M + M + + + + M + M + + + | 59 | Lactation | | 1 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + | 69 | Ulcer | | 1 3.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Skeletal Muscle | | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + | 69 | Compression | | 1 4.0| Mineralization, Focal | 2 | 2 1.5| Mineralization | 2 2 3 2 1 2 3 3 2 2 3 1 | 42 1.9| _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + | 68 | Congestion | | 1 2.0| Hemorrhage, Focal | 2 | 2 3.0| Inflammation, Chronic | | 1 3.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 12 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 4| 7| 4| 7| 2| 4| 2| 2| 7| 7| 2| 6| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 5| 4| 5| 4| 8| 5| 8| 8| 4| 4| 8| 8| 4| 4| 4| 4| 4| 4| 4| | | | 6| 7| 6| 7| 0| 5| 1| 1| 7| 7| 0| 4| 7| 7| 7| 7| 7| 7| 7| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 0 PPM | 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| | L | | 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - cont | | | | | | Peribronchial, Inflammation, Chronic | 1 1 | 3 1.3| __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + | 69 | Degeneration, Hyaline | 2 2 2 | 8 1.9| Inflammation, Chronic, Focal | 2 2 2 2 | 10 1.9| Inflammation, Chronic | 2 2 2 2 2 2 3 2 2 1 2 2 2 | 54 2.0| __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + | 69 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Ear | | 1 | __________________________________________________________________________|____________| Harderian Gland | + | 2 | Hyperplasia | | 1 2.0| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + | 69 | Fatty Change | | 2 2.0| Glomerulosclerosis | | 2 3.0| Hemorrhage, Focal | | 1 3.0| Hyperplasia, Lymphoid | 1 | 1 1.0| Infarct | 2 3 | 2 2.5| Mineralization | | 1 4.0| Renal Tubule, Degeneration, Granular | | 1 3.0| Renal Tubule, Regeneration | 1 1 1 | 7 1.3| __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + M + + + + + + + + + + | 63 | Inflammation, Chronic, Focal | 2 | 8 1.8| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 13 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 6| 4| 7| 7| 2| 7| 7| 4| 4| 2| 7| 7| 7| 7| 2| 7| 0| 6| 2| 7| 7| 7| 4| 4| 4| DAY ON TEST | 9| 5| 4| 4| 8| 4| 4| 5| 5| 8| 4| 4| 4| 4| 8| 4| 1| 8| 8| 4| 4| 4| 6| 5| 5| | 9| 6| 3| 3| 1| 3| 3| 5| 5| 1| 3| 3| 3| 1| 0| 3| 2| 1| 1| 3| 3| 6| 2| 5| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 50 PPM | 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| | 6| 7| 8| 9| 0| 1| 2| 3| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + M + + + + + + + + + + + M + + M + + + + + + +| Dilatation | | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | 3 4 3 2 3 3 2 2 | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Basophilic Focus | X X X | Clear Cell Focus | | Eosinophilic Focus | X | Fatty Change | | Hematopoietic Cell Proliferation | 2 | Hemorrhage | 2 | Hyperplasia, Lymphoid | | Necrosis, Focal | 2 2 | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2| Acinus, Atrophy | 4 | Duct, Ectasia | 3 | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2 2 2 2 2| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + +| Edema | | Erosion | 3 | Inflammation, Chronic | 2 | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + +| Artery, Inflammation, Chronic | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + +| Cytoplasmic Alteration, Focal | | Hypertrophy | | __________________________________________________________________________| Adrenal Medulla | + + + + I + + + + + + + + + + + + I + + + + + +| Hyperplasia, Focal | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 4 3 3 | _____________________________________________________________________________________________________________________| Page 14 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 6| 4| 7| 7| 2| 7| 7| 4| 4| 2| 7| 7| 7| 7| 2| 7| 0| 6| 2| 7| 7| 7| 4| 4| 4| DAY ON TEST | 9| 5| 4| 4| 8| 4| 4| 5| 5| 8| 4| 4| 4| 4| 8| 4| 1| 8| 8| 4| 4| 4| 6| 5| 5| | 9| 6| 3| 3| 1| 3| 3| 5| 5| 1| 3| 3| 3| 1| 0| 3| 2| 1| 1| 3| 3| 6| 2| 5| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 50 PPM | 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| | 6| 7| 8| 9| 0| 1| 2| 3| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Parathyroid Gland | M + + + + + + + + + + + M M M + + + + + + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + +| Pars Distalis, Angiectasis | | Pars Distalis, Hyperplasia, Focal | 4 4 3 3 3 | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic | | Follicular Cell, Hyperplasia, Focal | 2 2 3 2 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | + + M + + + + M + + + + + + + | Dilatation | 4 | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + +| Cyst | X X | Mineralization, Focal | | Thrombosis | X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Hydrometra | 3 | Endometrium, Hyperplasia, Cystic | 3 1 3 4 1 3 3 1 1 3 3 3 2 3 2 3 1 2 1 4 2 2| Endometrium, Metaplasia, Squamous | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Neutrophil | 4 2 | Myelofibrosis | 2 2 3 2 2 2 1 1 | __________________________________________________________________________| Lymph Node | + + | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + M + + + M + + + + M + + + + + + +| Congestion | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + +| Lymphatic, Angiectasis | 3 | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Lymphoid | | Hematopoietic Cell Proliferation | 4 2 | Hyperplasia, Lymphoid | | Necrosis, Focal | 3 | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Lymphoid | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + M + + + + + + + + + M + + + + + + + + + + +| Lactation | | _____________________________________________________________________________________________________________________| Page 15 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 6| 4| 7| 7| 2| 7| 7| 4| 4| 2| 7| 7| 7| 7| 2| 7| 0| 6| 2| 7| 7| 7| 4| 4| 4| DAY ON TEST | 9| 5| 4| 4| 8| 4| 4| 5| 5| 8| 4| 4| 4| 4| 8| 4| 1| 8| 8| 4| 4| 4| 6| 5| 5| | 9| 6| 3| 3| 1| 3| 3| 5| 5| 1| 3| 3| 3| 1| 0| 3| 2| 1| 1| 3| 3| 6| 2| 5| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 50 PPM | 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| | 6| 7| 8| 9| 0| 1| 2| 3| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM - cont | | | | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + +| Hyperostosis | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + +| Hemorrhage, Focal | | Mineralization | 2 2 3 2 1 2 3 2 3 2 2 1 1 2 1 1| Artery, Inflammation, Chronic | | __________________________________________________________________________| Spinal Cord | | Artery, Inflammation, Chronic | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | | Hemorrhage, Focal | 2 | Thrombosis | | Peribronchial, Inflammation, Chronic | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + +| Degeneration, Hyaline | 2 2 2 | Inflammation, Chronic, Focal | 2 2 2 | Inflammation, Chronic | 2 2 2 3 2 2 2 2 2 2 2 2 2 2 2 2 2| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | | __________________________________________________________________________| Harderian Gland | + | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | | Glomerulosclerosis | | Hyperplasia, Lymphoid | | Renal Tubule, Necrosis | | Renal Tubule, Regeneration | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + A + + + + + + + + + +| Inflammation, Chronic, Focal | 1 2 2 2 1| _____________________________________________________________________________________________________________________| Page 16 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 2| 7| 7| 7| 6| 4| 5| 7| 7| 7| 7| 7| 7| 1| 7| 7| 6| 2| 7| 3| 4| 6| 1| 7| DAY ON TEST | 4| 8| 4| 4| 4| 2| 5| 8| 4| 4| 4| 4| 4| 4| 0| 4| 4| 0| 8| 4| 9| 5| 9| 5| 4| | 6| 1| 6| 6| 3| 5| 5| 8| 3| 6| 6| 6| 6| 6| 3| 6| 6| 3| 1| 6| 1| 5| 0| 8| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 50 PPM | 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| | 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + M + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + A + A + + + + + + A + + A + + + + + A +| Dilatation | | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + A + + + + + + + + + A + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + A + + + + + + + + + A + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + A + + + + + + + + + A + + + + + A +| Hyperplasia, Lymphoid | 4 4 4 2 3 3 | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + A + + + + + + A + + A + + + + + A +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + A + + + + + + + + + A + + + + + A +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + A + A + + + + + + A + + A + + + + + A +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Basophilic Focus | X | Clear Cell Focus | X X | Eosinophilic Focus | X X | Fatty Change | 3 | Hematopoietic Cell Proliferation | | Hemorrhage | | Hyperplasia, Lymphoid | 1 | Necrosis, Focal | 2 2 | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2 | Acinus, Atrophy | | Duct, Ectasia | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 1 | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + A + + + + + + + + + +| Edema | 2 | Erosion | 2 | Inflammation, Chronic | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Artery, Inflammation, Chronic | 3 | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Cytoplasmic Alteration, Focal | 3 4 | Hypertrophy | | __________________________________________________________________________| Adrenal Medulla | + + + + + + + M + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 1 2 2 | _____________________________________________________________________________________________________________________| Page 17 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 2| 7| 7| 7| 6| 4| 5| 7| 7| 7| 7| 7| 7| 1| 7| 7| 6| 2| 7| 3| 4| 6| 1| 7| DAY ON TEST | 4| 8| 4| 4| 4| 2| 5| 8| 4| 4| 4| 4| 4| 4| 0| 4| 4| 0| 8| 4| 9| 5| 9| 5| 4| | 6| 1| 6| 6| 3| 5| 5| 8| 3| 6| 6| 6| 6| 6| 3| 6| 6| 3| 1| 6| 1| 5| 0| 8| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 50 PPM | 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| | 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Parathyroid Gland | + + + M + M + + + + + + + + + M + M + + M + + M +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Pars Distalis, Angiectasis | 2 | Pars Distalis, Hyperplasia, Focal | 4 2 3 | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + M + + + + + + +| Inflammation, Chronic | 4 | Follicular Cell, Hyperplasia, Focal | 2 2 4 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | + M + + + + + + + + + + + + M + + +| Dilatation | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Cyst | X X X X X | Mineralization, Focal | | Thrombosis | | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 2 4 | Hydrometra | 4 4 3 4 | Endometrium, Hyperplasia, Cystic | 2 3 2 1 4 1 2 1 2 3 4 3 3 1 3 3 2 3 2 2 2 3| Endometrium, Metaplasia, Squamous | 3 | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Neutrophil | 2 3 | Myelofibrosis | 1 2 4 2 1 1 1 | __________________________________________________________________________| Lymph Node | + + | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + M + + + + + + + + + M + + +| Congestion | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + A +| Lymphatic, Angiectasis | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Lymphoid | 2 3 | Hematopoietic Cell Proliferation | 3 4 3 4 4 | Hyperplasia, Lymphoid | 2 | Necrosis, Focal | | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Lymphoid | 4 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + M + + + + + + + + + + + M + + + M + + + + + + +| Lactation | X | _____________________________________________________________________________________________________________________| Page 18 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 2| 7| 7| 7| 6| 4| 5| 7| 7| 7| 7| 7| 7| 1| 7| 7| 6| 2| 7| 3| 4| 6| 1| 7| DAY ON TEST | 4| 8| 4| 4| 4| 2| 5| 8| 4| 4| 4| 4| 4| 4| 0| 4| 4| 0| 8| 4| 9| 5| 9| 5| 4| | 6| 1| 6| 6| 3| 5| 5| 8| 3| 6| 6| 6| 6| 6| 3| 6| 6| 3| 1| 6| 1| 5| 0| 8| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 50 PPM | 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| | 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM - cont | | | | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperostosis | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemorrhage, Focal | 3 | Mineralization | 2 2 2 3 2 1 2 3 3 2 2 3| Artery, Inflammation, Chronic | 4 | __________________________________________________________________________| Spinal Cord | + | Artery, Inflammation, Chronic | 3 | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | | Hemorrhage, Focal | 4 | Thrombosis | 1 | Peribronchial, Inflammation, Chronic | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Degeneration, Hyaline | 2 | Inflammation, Chronic, Focal | 2 2 | Inflammation, Chronic | 2 2 2 2 2 2 1 2 2 2 2 2 2 2 2 2 2 2 2| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | | __________________________________________________________________________| Harderian Gland | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | 3 | Glomerulosclerosis | | Hyperplasia, Lymphoid | | Renal Tubule, Necrosis | 4 3 | Renal Tubule, Regeneration | 1 1| __________________________________________________________________________| Urinary Bladder | + + + + + A + A + + + + + + A + + A + + + + + A +| Inflammation, Chronic, Focal | 3 | _____________________________________________________________________________________________________________________| Page 19 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 7| 4| 4| 4| 6| 2| 7| 7| 7| 7| 7| 1| 7| 2| 2| 7| 7| 6| 7| | | DAY ON TEST | 4| 5| 5| 5| 2| 8| 4| 4| 4| 4| 4| 4| 4| 8| 8| 4| 4| 9| 3| | | | 6| 6| 6| 6| 8| 0| 6| 6| 6| 6| 6| 5| 6| 1| 0| 6| 6| 0| 0| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | A | 50 PPM | 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| | L | | 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + | 67 | __________________________________________________________________________|____________| Gallbladder | + M M + A + + + + + + + + + + + + A + | 56 | Dilatation | 4 | 1 4.0| __________________________________________________________________________|____________| Intestine Large, Colon | + + + + A + + + + + + + + + + + + + + | 65 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + A + + + + + + + + + + + + + + | 65 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + A + + + + + + + + + + + + A + | 63 | Hyperplasia, Lymphoid | 3 4 2 2 | 18 2.9| __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + A + + + + + + + + + + + + A + | 62 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + A + + + + + + + + + + + + A + | 63 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + A + + + + + + + + + + + + A + | 61 | __________________________________________________________________________|____________| Liver | + + + + A + + + + + + + + + + + + + + | 67 | Angiectasis | 2 | 1 2.0| Basophilic Focus | | 4 | Clear Cell Focus | | 2 | Eosinophilic Focus | | 3 | Fatty Change | | 1 3.0| Hematopoietic Cell Proliferation | | 1 2.0| Hemorrhage | | 1 2.0| Hyperplasia, Lymphoid | | 1 1.0| Necrosis, Focal | | 4 2.0| __________________________________________________________________________|____________| Pancreas | + + + + A + + + + + + + + + + + + + + | 67 | Inflammation, Chronic, Focal | | 2 2.0| Acinus, Atrophy | | 1 4.0| Duct, Ectasia | | 1 3.0| __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + M + + + + | 67 | Inflammation, Chronic, Focal | 2 | 7 1.9| __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + A + + + + + + + + + + + + A + | 65 | Edema | | 1 2.0| Erosion | | 2 2.5| Inflammation, Chronic | | 1 2.0| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + | 68 | Artery, Inflammation, Chronic | | 1 3.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | + + + + A + + + + + + + + + + + + + + | 67 | Cytoplasmic Alteration, Focal | | 2 3.5| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 20 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 7| 4| 4| 4| 6| 2| 7| 7| 7| 7| 7| 1| 7| 2| 2| 7| 7| 6| 7| | | DAY ON TEST | 4| 5| 5| 5| 2| 8| 4| 4| 4| 4| 4| 4| 4| 8| 8| 4| 4| 9| 3| | | | 6| 6| 6| 6| 8| 0| 6| 6| 6| 6| 6| 5| 6| 1| 0| 6| 6| 0| 0| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | A | 50 PPM | 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| | L | | 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | Hypertrophy | 2 | 1 2.0| __________________________________________________________________________|____________| Adrenal Medulla | + + + + A + + + + + + + + + + + + + + | 64 | Hyperplasia, Focal | 3 | 1 3.0| __________________________________________________________________________|____________| Islets, Pancreatic | + + + + A + + + + + + + + + + + + + + | 67 | Hyperplasia | 4 2 | 9 2.6| __________________________________________________________________________|____________| Parathyroid Gland | + M + M M M + + M M M M + + + + M M + | 48 | __________________________________________________________________________|____________| Pituitary Gland | + + M + + + + + M + + + + + + + + + + | 66 | Pars Distalis, Angiectasis | | 1 2.0| Pars Distalis, Hyperplasia, Focal | 4 3 | 10 3.3| __________________________________________________________________________|____________| Thyroid Gland | + + M + + + + + + + + + + + + + + + + | 66 | Inflammation, Chronic | | 1 4.0| Follicular Cell, Hyperplasia, Focal | 2 2 1 1 | 11 2.1| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Clitoral Gland | + + + + + + + + + + + + | 41 | Dilatation | | 1 4.0| __________________________________________________________________________|____________| Ovary | + + + + A + + + + + + + + + + + + + + | 67 | Cyst | X X X X X X | 13 | Mineralization, Focal | 3 | 1 3.0| Thrombosis | | 1 | __________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + | 68 | Angiectasis | 2 | 3 2.7| Hydrometra | 2 4 4 | 8 3.5| Endometrium, Hyperplasia, Cystic | 3 2 2 3 3 2 4 3 4 3 4 3 2 2 3 4 1 3 | 62 2.5| Endometrium, Metaplasia, Squamous | | 1 3.0| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + | 68 | Hyperplasia, Neutrophil | 3 4 | 6 3.0| Myelofibrosis | 2 1 2 3 1 1 | 21 1.8| __________________________________________________________________________|____________| Lymph Node | + + + | 7 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + A + + + + + + + + + M + + + + | 61 | Congestion | 2 | 1 2.0| __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + A + + + + + + + + + + + + + + | 66 | Lymphatic, Angiectasis | | 1 3.0| __________________________________________________________________________|____________| Spleen | + + + + A + + + + + + + + + + + + + + | 67 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 21 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 7| 4| 4| 4| 6| 2| 7| 7| 7| 7| 7| 1| 7| 2| 2| 7| 7| 6| 7| | | DAY ON TEST | 4| 5| 5| 5| 2| 8| 4| 4| 4| 4| 4| 4| 4| 8| 8| 4| 4| 9| 3| | | | 6| 6| 6| 6| 8| 0| 6| 6| 6| 6| 6| 5| 6| 1| 0| 6| 6| 0| 0| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | A | 50 PPM | 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| | L | | 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Depletion Lymphoid | 4 | 3 3.0| Hematopoietic Cell Proliferation | 3 1 4 | 10 3.2| Hyperplasia, Lymphoid | | 1 2.0| Necrosis, Focal | | 1 3.0| __________________________________________________________________________|____________| Thymus | + + + + A + + + + + + + + + + + + + M | 66 | Depletion Lymphoid | 4 | 2 4.0| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + M + + + M + + + M | 60 | Lactation | X | 2 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + | 68 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + | 68 | Hyperostosis | 2 | 1 2.0| _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + | 68 | Hemorrhage, Focal | | 1 3.0| Mineralization | 2 2 3 1 3 3 3 2 1 2 | 38 2.1| Artery, Inflammation, Chronic | | 1 4.0| __________________________________________________________________________|____________| Spinal Cord | | 1 | Artery, Inflammation, Chronic | | 1 3.0| _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + | 68 | Congestion | 3 | 1 3.0| Hemorrhage, Focal | | 2 3.0| Thrombosis | | 1 1.0| Peribronchial, Inflammation, Chronic | 1 | 1 1.0| __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + | 68 | Degeneration, Hyaline | | 4 2.0| Inflammation, Chronic, Focal | 2 2 2 | 8 2.0| Inflammation, Chronic | 2 2 2 2 2 2 2 2 2 2 2 1 2 | 49 2.0| __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + | 68 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | + | 1 | __________________________________________________________________________|____________| Harderian Gland | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 22 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 7| 4| 4| 4| 6| 2| 7| 7| 7| 7| 7| 1| 7| 2| 2| 7| 7| 6| 7| | | DAY ON TEST | 4| 5| 5| 5| 2| 8| 4| 4| 4| 4| 4| 4| 4| 8| 8| 4| 4| 9| 3| | | | 6| 6| 6| 6| 8| 0| 6| 6| 6| 6| 6| 5| 6| 1| 0| 6| 6| 0| 0| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | A | 50 PPM | 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| | L | | 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ SPECIAL SENSES SYSTEM - cont | | | | | | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + A + + + + + + + + + + + + + + | 67 | Congestion | | 1 3.0| Glomerulosclerosis | 4 3 | 2 3.5| Hyperplasia, Lymphoid | 2 | 1 2.0| Renal Tubule, Necrosis | | 2 3.5| Renal Tubule, Regeneration | 2 | 3 1.3| __________________________________________________________________________|____________| Urinary Bladder | + + + + A + + + M + + + + + + + + A + | 59 | Inflammation, Chronic, Focal | 1 | 7 1.7| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 23 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 6| 4| 2| 7| 7| 7| 4| 7| 6| 7| 7| 0| 7| 7| 7| 7| 7| 7| 6| 1| 2| DAY ON TEST | 4| 1| 4| 4| 9| 5| 8| 4| 4| 4| 5| 0| 8| 4| 4| 5| 4| 4| 4| 4| 4| 4| 8| 8| 8| | 3| 9| 3| 3| 7| 6| 0| 2| 3| 2| 5| 8| 9| 2| 3| 7| 2| 2| 2| 2| 2| 3| 6| 3| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 250 PPM | 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + A + + + + + + + + + + + + + A + + + M + + + + +| __________________________________________________________________________| Intestine Large, Colon | + A + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + A + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + A + + + + + + + + + + + + + A + + + + + + + + +| Edema | 3 | Hyperplasia, Lymphoid | 3 3 3 2 3 2 3 2 | __________________________________________________________________________| Intestine Small, Duodenum | + A + + + + + + + + + + + + + A + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + A + + + + + + + + + + + + + A + + + + + + + + +| Hyperplasia, Lymphoid | 4 | __________________________________________________________________________| Intestine Small, Ileum | + A + + + + + + + + + + + + + A + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Basophilic Focus | | Clear Cell Focus | X | Eosinophilic Focus | X X X X X | Fatty Change, Focal | 1 1 | Fatty Change | 2 1 1 | Hematopoietic Cell Proliferation | | Necrosis, Focal | 1 2 | __________________________________________________________________________| Mesentery | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | | Acinus, Atrophy | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Diverticulum | | Ulcer | 4 | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2 | Inflammation, Chronic | | Muscularis, Hypertrophy | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + M + + + + + + + + + + + + + +| Hypertrophy | 2 | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + M + + + + + + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 2 1 | Hypoplasia | 2| _____________________________________________________________________________________________________________________| Page 24 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 6| 4| 2| 7| 7| 7| 4| 7| 6| 7| 7| 0| 7| 7| 7| 7| 7| 7| 6| 1| 2| DAY ON TEST | 4| 1| 4| 4| 9| 5| 8| 4| 4| 4| 5| 0| 8| 4| 4| 5| 4| 4| 4| 4| 4| 4| 8| 8| 8| | 3| 9| 3| 3| 7| 6| 0| 2| 3| 2| 5| 8| 9| 2| 3| 7| 2| 2| 2| 2| 2| 3| 6| 3| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 250 PPM | 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Parathyroid Gland | + + + + + M + M + + + + + + + + + + + + + + M + M| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Pars Distalis, Hyperplasia, Focal | 2 3 3 3 4 2 3 3 | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicle, Cyst | X | Follicular Cell, Hyperplasia, Focal | 1 1 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | + + + + + + + + + + + M + + + + + + + | Abscess | | Pigmentation, Hemosiderin | 2 | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Abscess | 4 | Angiectasis | | Cyst | X X X X | Mineralization, Focal | | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Hydrometra | 4 4 | Endometrium, Hyperplasia, Cystic | 4 4 3 2 1 2 3 3 4 2 3 3 2 3 3 4 3 3 3 2 3 2 2| Endometrium, Metaplasia, Squamous | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Neutrophil | 4 | Myelofibrosis | 1 2 1 3 2 3 1 2 2 2 | __________________________________________________________________________| Lymph Node | + + + + | Lumbar, Lymphatic, Angiectasis | | Mediastinal, Infiltration Cellular, | | Mixed Cell | | Pancreatic, Hyperplasia, Lymphoid | 2 1 | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| Necrosis | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + M + + +| Lymphatic, Angiectasis | 3 | __________________________________________________________________________| Spleen | + + + + + + M + + + + + + + + + + + + + + + + + +| Congestion | 3 | Depletion Lymphoid | 2 4 | Hematopoietic Cell Proliferation | 4 2 | Hyperplasia, Lymphoid | 2 1 | Necrosis, Focal | | _____________________________________________________________________________________________________________________| Page 25 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 6| 4| 2| 7| 7| 7| 4| 7| 6| 7| 7| 0| 7| 7| 7| 7| 7| 7| 6| 1| 2| DAY ON TEST | 4| 1| 4| 4| 9| 5| 8| 4| 4| 4| 5| 0| 8| 4| 4| 5| 4| 4| 4| 4| 4| 4| 8| 8| 8| | 3| 9| 3| 3| 7| 6| 0| 2| 3| 2| 5| 8| 9| 2| 3| 7| 2| 2| 2| 2| 2| 3| 6| 3| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 250 PPM | 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Lymphoid | 3 3 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + M + + + + + + + + + + + + + + + + M M| Lactation | X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Compression | 3 | Mineralization, Focal | 1| Mineralization | 2 2 2 1 2 2 2 1 2 1 2 1 | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemorrhage, Focal | | Inflammation, Chronic | | Peribronchial, Inflammation, Chronic | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Degeneration, Hyaline | 2 2| Inflammation, Chronic, Focal | 2 2| Inflammation, Chronic | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | | __________________________________________________________________________| Harderian Gland | + + + | Hyperplasia | 2 | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | 2 | Hyperplasia, Lymphoid | | Infarct | | Inflammation, Chronic, Focal | | Nephropathy | 2 | Renal Tubule, Degeneration, Granular | | Renal Tubule, Regeneration | 1 1| __________________________________________________________________________| Urinary Bladder | + A + + + + + + + + + + + M + A + + + + + + + + +| Inflammation, Chronic, Focal | 1 1 2| _____________________________________________________________________________________________________________________| Page 26 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 6| 7| 2| 5| 4| 7| 7| 6| 7| 2| 2| 7| 2| 7| 7| 4| 7| 4| 2| 4| 4| 7| 7| 7| DAY ON TEST | 4| 5| 4| 8| 7| 5| 4| 2| 0| 4| 8| 8| 4| 8| 4| 0| 5| 4| 5| 8| 5| 6| 4| 4| 4| | 2| 6| 2| 1| 8| 5| 3| 8| 7| 3| 0| 0| 3| 1| 3| 5| 5| 2| 5| 0| 6| 9| 2| 2| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 250 PPM | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + A + + M + + + A + + + + + + A + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + A + + + + + + A + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + A + + + + + + A + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + A + + + + + + A + + + + + + A + + + + + + + + +| Edema | | Hyperplasia, Lymphoid | 3 2 3 | __________________________________________________________________________| Intestine Small, Duodenum | + A + + + + + + A + + + + + + A + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + A + + + + + + A + + + + + + A + + + + + + + + +| Hyperplasia, Lymphoid | | __________________________________________________________________________| Intestine Small, Ileum | + A + + + + + + A + + + + + + A + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + A + + + + + + + + + + + + + + + +| Angiectasis | 1 | Basophilic Focus | X X | Clear Cell Focus | X | Eosinophilic Focus | X X X| Fatty Change, Focal | | Fatty Change | 1 2 | Hematopoietic Cell Proliferation | 3 2 | Necrosis, Focal | 4 3 1 1 | __________________________________________________________________________| Mesentery | + | __________________________________________________________________________| Pancreas | + + + + + + + + A + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2 | Acinus, Atrophy | 2 | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2 2 2 | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + A + + + + + + + + + + + + + + + +| Diverticulum | 2 | Ulcer | | __________________________________________________________________________| Stomach, Glandular | + A + + + + + + A + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | | Inflammation, Chronic | | Muscularis, Hypertrophy | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + A + + + + + + + + + + + + + + + +| Hypertrophy | | __________________________________________________________________________| Adrenal Medulla | + + + M + + + + A + + + + M + + + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + A + + + + + + + + + + + + + + + +| Hyperplasia | 3 | Hypoplasia | | _____________________________________________________________________________________________________________________| Page 27 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 6| 7| 2| 5| 4| 7| 7| 6| 7| 2| 2| 7| 2| 7| 7| 4| 7| 4| 2| 4| 4| 7| 7| 7| DAY ON TEST | 4| 5| 4| 8| 7| 5| 4| 2| 0| 4| 8| 8| 4| 8| 4| 0| 5| 4| 5| 8| 5| 6| 4| 4| 4| | 2| 6| 2| 1| 8| 5| 3| 8| 7| 3| 0| 0| 3| 1| 3| 5| 5| 2| 5| 0| 6| 9| 2| 2| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 250 PPM | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Parathyroid Gland | + M M M M M + + M + + M + M + + + + + + + + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + M + + + + + + + + + + + + + +| Pars Distalis, Hyperplasia, Focal | 4 4 3 3| __________________________________________________________________________| Thyroid Gland | + + + + + + + + M + + + + + + + + + + + + + + + +| Follicle, Cyst | | Follicular Cell, Hyperplasia, Focal | 1 2 1 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | + + + + + + A + + + + + + + + +| Abscess | | Pigmentation, Hemosiderin | | __________________________________________________________________________| Ovary | + + + + + + + + M + + + + + + + + + + + + + + + +| Abscess | | Angiectasis | | Cyst | X X X X X | Mineralization, Focal | 4 | __________________________________________________________________________| Uterus | + + + + + + + + A + + + + + + + + + + + + + + + +| Angiectasis | | Hydrometra | 4 | Endometrium, Hyperplasia, Cystic | 3 3 2 4 1 3 2 2 4 2 4 3 2 2 2 1 2 3 4 3| Endometrium, Metaplasia, Squamous | 3 | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + A + + + + + + A + + M + + + + + + + + + + + + +| Hyperplasia, Neutrophil | 4 | Myelofibrosis | 1 1 1 3 2 3 1| __________________________________________________________________________| Lymph Node | + + | Lumbar, Lymphatic, Angiectasis | | Mediastinal, Infiltration Cellular, | | Mixed Cell | | Pancreatic, Hyperplasia, Lymphoid | | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + A + + + + + + + + + M + + + + + +| Necrosis | 4 | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + M A + + + + + + + + + + + + + + + +| Lymphatic, Angiectasis | 4 | __________________________________________________________________________| Spleen | + + + + + + + + M + + + + + + + + + + + + + + + +| Congestion | | Depletion Lymphoid | | Hematopoietic Cell Proliferation | 4 3 3 2 3 3 | Hyperplasia, Lymphoid | 3 | Necrosis, Focal | 4 | _____________________________________________________________________________________________________________________| Page 28 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 6| 7| 2| 5| 4| 7| 7| 6| 7| 2| 2| 7| 2| 7| 7| 4| 7| 4| 2| 4| 4| 7| 7| 7| DAY ON TEST | 4| 5| 4| 8| 7| 5| 4| 2| 0| 4| 8| 8| 4| 8| 4| 0| 5| 4| 5| 8| 5| 6| 4| 4| 4| | 2| 6| 2| 1| 8| 5| 3| 8| 7| 3| 0| 0| 3| 1| 3| 5| 5| 2| 5| 0| 6| 9| 2| 2| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 250 PPM | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | __________________________________________________________________________| Thymus | + + + + + + + + M + + + + M + + + + + + + + + + +| Depletion Lymphoid | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + M + + + M + + + + + + + + + + + +| Lactation | | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Compression | | Mineralization, Focal | | Mineralization | 3 2 1 2 3 2 1 2 1 2 1 1 2| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemorrhage, Focal | 3 | Inflammation, Chronic | | Peribronchial, Inflammation, Chronic | 1 2 2 | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Degeneration, Hyaline | 2 2 2 2 | Inflammation, Chronic, Focal | 2 2 2 2 2 | Inflammation, Chronic | 2 2 1 2 2 2 2 2 2 2 2 2 2 2 | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | | __________________________________________________________________________| Harderian Gland | | Hyperplasia | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | | Hyperplasia, Lymphoid | 2 | Infarct | 3 | Inflammation, Chronic, Focal | | Nephropathy | | Renal Tubule, Degeneration, Granular | 4 4 | Renal Tubule, Regeneration | | __________________________________________________________________________| Urinary Bladder | + A + + + + + + A + + + + + + A + + + + + + + + +| Inflammation, Chronic, Focal | 1 2 1 2 2 | _____________________________________________________________________________________________________________________| Page 29 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 4| 7| 2| 7| 7| 7| 4| 4| 7| 4| 7| 7| 7| 7| 2| 7| 7| 2| 7| 7| | | DAY ON TEST | 5| 4| 8| 4| 4| 4| 5| 5| 4| 5| 4| 4| 4| 4| 8| 4| 4| 8| 4| 4| | | | 5| 2| 1| 2| 2| 2| 5| 6| 3| 6| 3| 2| 2| 2| 1| 3| 3| 0| 2| 2| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| | A | 250 PPM | 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| | L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + M + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + + + + + | 63 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + | 67 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + | 67 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + | 65 | Edema | | 1 3.0| Hyperplasia, Lymphoid | 2 1 4 3 2 3 4 | 18 2.7| __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + | 65 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + | 65 | Hyperplasia, Lymphoid | | 1 4.0| __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + | 65 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + | 69 | Angiectasis | | 1 1.0| Basophilic Focus | X | 3 | Clear Cell Focus | | 2 | Eosinophilic Focus | X X | 10 | Fatty Change, Focal | | 2 1.0| Fatty Change | 1 1 1 | 8 1.3| Hematopoietic Cell Proliferation | | 2 2.5| Necrosis, Focal | 2 | 7 2.0| __________________________________________________________________________|____________| Mesentery | | 1 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + | 69 | Inflammation, Chronic, Focal | | 1 2.0| Acinus, Atrophy | | 1 2.0| __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + | 70 | Inflammation, Chronic, Focal | 1 2 2 2 | 7 1.9| __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + | 69 | Diverticulum | | 1 2.0| Ulcer | | 1 4.0| __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + | 68 | Inflammation, Chronic, Focal | | 1 2.0| Inflammation, Chronic | 2 | 1 2.0| Muscularis, Hypertrophy | 3 | 1 3.0| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + M + + + + + + + + + + | 69 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 30 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 4| 7| 2| 7| 7| 7| 4| 4| 7| 4| 7| 7| 7| 7| 2| 7| 7| 2| 7| 7| | | DAY ON TEST | 5| 4| 8| 4| 4| 4| 5| 5| 4| 5| 4| 4| 4| 4| 8| 4| 4| 8| 4| 4| | | | 5| 2| 1| 2| 2| 2| 5| 6| 3| 6| 3| 2| 2| 2| 1| 3| 3| 0| 2| 2| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| | A | 250 PPM | 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| | L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | Hypertrophy | 2 | 2 2.0| __________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + | 66 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + | 69 | Hyperplasia | 1 1 2 2 2 4 | 10 2.0| Hypoplasia | | 1 2.0| __________________________________________________________________________|____________| Parathyroid Gland | + + + + M + + + + M + + + M + + M + + M | 53 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + M + + + + | 68 | Pars Distalis, Hyperplasia, Focal | 3 | 13 3.1| __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + M + + + + + + + + + + | 68 | Follicle, Cyst | X | 2 | Follicular Cell, Hyperplasia, Focal | 1 1 3 | 8 1.4| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Clitoral Gland | + + M + + + M + + + + + + | 44 | Abscess | 2 | 1 2.0| Pigmentation, Hemosiderin | | 1 2.0| __________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + | 69 | Abscess | | 1 4.0| Angiectasis | 3 | 1 3.0| Cyst | X X X X | 13 | Mineralization, Focal | | 1 4.0| __________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + | 69 | Angiectasis | 1 3 | 2 2.0| Hydrometra | 4 4 4 | 6 4.0| Endometrium, Hyperplasia, Cystic | 1 2 2 2 1 2 3 1 3 2 3 3 4 3 3 | 58 2.6| Endometrium, Metaplasia, Squamous | 3 | 2 3.0| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + | 67 | Hyperplasia, Neutrophil | 2 3 3 | 5 3.2| Myelofibrosis | 1 1 1 1 2 | 22 1.7| __________________________________________________________________________|____________| Lymph Node | + + | 8 | Lumbar, Lymphatic, Angiectasis | 3 | 1 3.0| Mediastinal, Infiltration Cellular, | | | Mixed Cell | 3 | 1 3.0| Pancreatic, Hyperplasia, Lymphoid | | 2 1.5| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 31 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 4| 7| 2| 7| 7| 7| 4| 4| 7| 4| 7| 7| 7| 7| 2| 7| 7| 2| 7| 7| | | DAY ON TEST | 5| 4| 8| 4| 4| 4| 5| 5| 4| 5| 4| 4| 4| 4| 8| 4| 4| 8| 4| 4| | | | 5| 2| 1| 2| 2| 2| 5| 6| 3| 6| 3| 2| 2| 2| 1| 3| 3| 0| 2| 2| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| | A | 250 PPM | 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| | L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + | 68 | Necrosis | | 1 4.0| __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + | 67 | Lymphatic, Angiectasis | 3 | 3 3.3| __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + | 68 | Congestion | | 1 3.0| Depletion Lymphoid | 1 | 3 2.3| Hematopoietic Cell Proliferation | 3 4 2 3 4 | 13 3.1| Hyperplasia, Lymphoid | 2 | 4 2.0| Necrosis, Focal | | 1 4.0| __________________________________________________________________________|____________| Thymus | + + + + + + + + + M + + + + + + + + + + | 67 | Depletion Lymphoid | | 2 3.0| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | + + + + M + + + + + + + + + + + + M + + | 63 | Lactation | X X | 3 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + | 70 | Compression | | 1 3.0| Mineralization, Focal | | 1 1.0| Mineralization | 2 2 3 2 2 1 2 2 2 2 1 | 36 1.8| _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | + + + + + + + + + M + + + + + + + + + + | 69 | Hemorrhage, Focal | | 1 3.0| Inflammation, Chronic | 2 | 1 2.0| Peribronchial, Inflammation, Chronic | 2 1 | 5 1.6| __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + | 70 | Degeneration, Hyaline | 2 | 7 2.0| Inflammation, Chronic, Focal | 2 2 2 | 10 2.0| Inflammation, Chronic | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 | 51 2.0| __________________________________________________________________________|____________| Trachea | + + + + + + + + + M + + + + + + + + + + | 69 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 32 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 4| 7| 2| 7| 7| 7| 4| 4| 7| 4| 7| 7| 7| 7| 2| 7| 7| 2| 7| 7| | | DAY ON TEST | 5| 4| 8| 4| 4| 4| 5| 5| 4| 5| 4| 4| 4| 4| 8| 4| 4| 8| 4| 4| | | | 5| 2| 1| 2| 2| 2| 5| 6| 3| 6| 3| 2| 2| 2| 1| 3| 3| 0| 2| 2| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| | A | 250 PPM | 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| | L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ SPECIAL SENSES SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Ear | + + | 2 | __________________________________________________________________________|____________| Harderian Gland | + | 4 | Hyperplasia | | 1 2.0| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + | 70 | Congestion | | 1 2.0| Hyperplasia, Lymphoid | | 1 2.0| Infarct | 2 | 2 2.5| Inflammation, Chronic, Focal | 2 | 1 2.0| Nephropathy | | 1 2.0| Renal Tubule, Degeneration, Granular | | 2 4.0| Renal Tubule, Regeneration | 1 | 3 1.0| __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + | 64 | Inflammation, Chronic, Focal | 2 | 9 1.6| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 33 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 7| 7| 2| 4| 6| 4| 7| 7| 7| 7| 7| 7| 4| 7| 6| 7| 4| 2| 7| 7| 7| 2| 6| 7| DAY ON TEST | 4| 4| 4| 8| 5| 7| 5| 4| 4| 4| 4| 4| 4| 5| 4| 9| 4| 5| 8| 4| 4| 4| 8| 4| 4| | 1| 2| 2| 0| 6| 7| 5| 2| 2| 2| 2| 2| 1| 6| 1| 5| 1| 6| 1| 1| 1| 1| 1| 8| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 500 PPM | 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Dilatation | 4 | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | 2 3 2 2 2 3 3 | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + M + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | X | Eosinophilic Focus | X X X X X X X X | Fatty Change | 1 1 | Necrosis, Focal | 1 1 1 2 1 1 2 4 2 | __________________________________________________________________________| Mesentery | + + | Fat, Necrosis | 3 3 | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | | Acinus, Atrophy | 4 | Duct, Ectasia | 4 | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2 1 | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Subacute | | __________________________________________________________________________| Tongue | | Angiectasis | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Fibrosis | | Hematopoietic Cell Proliferation | 4 | Hyperplasia, Focal | | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + M + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 1 2 1 | __________________________________________________________________________| Parathyroid Gland | + + + + M + M + + + + + M + + M M + + + + + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + M + + + + + + + + + + + + + + + + + + M| Pars Distalis, Angiectasis | | _____________________________________________________________________________________________________________________| Page 34 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 7| 7| 2| 4| 6| 4| 7| 7| 7| 7| 7| 7| 4| 7| 6| 7| 4| 2| 7| 7| 7| 2| 6| 7| DAY ON TEST | 4| 4| 4| 8| 5| 7| 5| 4| 4| 4| 4| 4| 4| 5| 4| 9| 4| 5| 8| 4| 4| 4| 8| 4| 4| | 1| 2| 2| 0| 6| 7| 5| 2| 2| 2| 2| 2| 1| 6| 1| 5| 1| 6| 1| 1| 1| 1| 1| 8| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 500 PPM | 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Pars Distalis, Hyperplasia, Focal | 1 2 | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Acute, Focal | | Follicle, Cyst | | Follicular Cell, Hyperplasia, Focal | 1 4 1 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | + + + M + + + + + + + + + + + + + +| Angiectasis | 3 | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + M + + + + + + + + +| Cyst | X X X X| __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 3 | Fibrosis, Focal | 2 | Hydrometra | 4 4 2 4 3 2 | Inflammation, Suppurative | 4 4 | Thrombosis | | Endometrium, Hyperplasia, Cystic | 1 2 1 3 2 2 3 1 1 3 4 1 3 1 2 1 2 2 3 2 1 3| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 4 | Hyperplasia, Neutrophil | 4 | Myelofibrosis | 1 1 3 1 2 2 | __________________________________________________________________________| Lymph Node | + +| __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphatic, Angiectasis | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + M + + + + + + + + +| Lymphatic, Angiectasis | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | 2 2 | Depletion Lymphoid | | Hematopoietic Cell Proliferation | 3 4 4 3 4 | Hyperplasia, Lymphoid | 2 3 | __________________________________________________________________________| Thymus | + + + + + M + + + + + + + + + + + + + + + + + + +| Angiectasis | | Hyperplasia, Lymphoid | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + M + + M + + + + + + + + + +| Lactation | X | _____________________________________________________________________________________________________________________| Page 35 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 7| 7| 2| 4| 6| 4| 7| 7| 7| 7| 7| 7| 4| 7| 6| 7| 4| 2| 7| 7| 7| 2| 6| 7| DAY ON TEST | 4| 4| 4| 8| 5| 7| 5| 4| 4| 4| 4| 4| 4| 5| 4| 9| 4| 5| 8| 4| 4| 4| 8| 4| 4| | 1| 2| 2| 0| 6| 7| 5| 2| 2| 2| 2| 2| 1| 6| 1| 5| 1| 6| 1| 1| 1| 1| 1| 8| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 500 PPM | 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM - cont | | | | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Mineralization, Focal | | Mineralization | 2 2 1 2 2 2 1 2 2 2 1 1 | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar Epithelium, Hyperplasia | 1 1 | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Degeneration, Hyaline | 2 2 2 | Inflammation, Chronic, Focal | 2 2 2 | Inflammation, Chronic | 2 2 2 2 2 2 2 2 2 2 2 2 1 2 2 2 2 2 2 2| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Harderian Gland | + + | Hyperplasia | 4 | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Glomerulosclerosis | | Infarct | 4 2 | Mineralization | 1 | Nephropathy | | Renal Tubule, Regeneration | 2 2 | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 1 3 | _____________________________________________________________________________________________________________________| Page 36 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 4| 7| 2| 7| 7| 4| 7| 7| 6| 7| 2| 4| 7| 4| 7| 2| 4| 2| 7| 7| 7| 4| 7| 2| 7| DAY ON TEST | 5| 4| 8| 4| 4| 5| 4| 4| 9| 4| 8| 5| 4| 5| 4| 8| 5| 8| 4| 4| 4| 5| 4| 8| 4| | 5| 1| 1| 1| 2| 5| 1| 1| 0| 1| 0| 5| 1| 6| 1| 1| 6| 0| 1| 1| 1| 6| 1| 0| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 500 PPM | 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + M + + + + + + + + + + + + + + + +| Dilatation | | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | 3 2 2 | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | | Eosinophilic Focus | X X X X X X X X X X | Fatty Change | 1 2 1 | Necrosis, Focal | 3 1 2 | __________________________________________________________________________| Mesentery | | Fat, Necrosis | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2 | Acinus, Atrophy | | Duct, Ectasia | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 1 1 2 2 1 2 4 | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Subacute | | __________________________________________________________________________| Tongue | + | Angiectasis | 3 | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Fibrosis | | Hematopoietic Cell Proliferation | | Hyperplasia, Focal | | __________________________________________________________________________| Adrenal Medulla | + + M + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 1 2 | __________________________________________________________________________| Parathyroid Gland | + + M + M + M M + M M M + M + + M + + + + + + + M| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + M + + + +| Pars Distalis, Angiectasis | 2| _____________________________________________________________________________________________________________________| Page 37 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 4| 7| 2| 7| 7| 4| 7| 7| 6| 7| 2| 4| 7| 4| 7| 2| 4| 2| 7| 7| 7| 4| 7| 2| 7| DAY ON TEST | 5| 4| 8| 4| 4| 5| 4| 4| 9| 4| 8| 5| 4| 5| 4| 8| 5| 8| 4| 4| 4| 5| 4| 8| 4| | 5| 1| 1| 1| 2| 5| 1| 1| 0| 1| 0| 5| 1| 6| 1| 1| 6| 0| 1| 1| 1| 6| 1| 0| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 500 PPM | 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Pars Distalis, Hyperplasia, Focal | 2 2 2 | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + M + + + + + + + + + + + + + + +| Inflammation, Acute, Focal | 1 | Follicle, Cyst | X | Follicular Cell, Hyperplasia, Focal | 1 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | + + + + + + + + + M + + + +| Angiectasis | | __________________________________________________________________________| Ovary | + + I + + + + + + + + + + + + + + + + + + + + + +| Cyst | X X X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Fibrosis, Focal | | Hydrometra | 4 3 2 | Inflammation, Suppurative | | Thrombosis | X | Endometrium, Hyperplasia, Cystic | 1 2 1 2 1 2 2 4 2 2 3 2 3 1 4 2 2 3 1 4 1 3 3| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Hyperplasia, Neutrophil | | Myelofibrosis | 1 2 1 1 1 1 2| __________________________________________________________________________| Lymph Node | + + | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + M + + +| Lymphatic, Angiectasis | 4 | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphatic, Angiectasis | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | | Depletion Lymphoid | 1 | Hematopoietic Cell Proliferation | 4 3 | Hyperplasia, Lymphoid | | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Hyperplasia, Lymphoid | 2 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + M + + + + + + + + + + + + + + +| Lactation | | _____________________________________________________________________________________________________________________| Page 38 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 4| 7| 2| 7| 7| 4| 7| 7| 6| 7| 2| 4| 7| 4| 7| 2| 4| 2| 7| 7| 7| 4| 7| 2| 7| DAY ON TEST | 5| 4| 8| 4| 4| 5| 4| 4| 9| 4| 8| 5| 4| 5| 4| 8| 5| 8| 4| 4| 4| 5| 4| 8| 4| | 5| 1| 1| 1| 2| 5| 1| 1| 0| 1| 0| 5| 1| 6| 1| 1| 6| 0| 1| 1| 1| 6| 1| 0| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 500 PPM | 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM - cont | | | | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Mineralization, Focal | 1 | Mineralization | 1 2 1 3 1 2 1 2 1 3 2 2 2 2| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar Epithelium, Hyperplasia | 1 1 | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Degeneration, Hyaline | 2 2 2 | Inflammation, Chronic, Focal | 2 2 2 2 2 | Inflammation, Chronic | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Harderian Gland | + | Hyperplasia | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Glomerulosclerosis | | Infarct | 2 | Mineralization | | Nephropathy | | Renal Tubule, Regeneration | 1 | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 1 2 1 2 2 2 | _____________________________________________________________________________________________________________________| Page 39 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 2| 2| | | DAY ON TEST | 4| 6| 4| 4| 6| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 3| 8| 8| | | | 1| 0| 1| 1| 0| 1| 2| 1| 1| 1| 1| 1| 1| 2| 1| 1| 1| 9| 0| 0| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | A | 500 PPM | 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + M + + + + + + | 68 | Dilatation | | 1 4.0| __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + | 70 | Hyperplasia, Lymphoid | 3 2 2 4 1 2 3 | 17 2.4| __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + | 70 | Hyperplasia, Lymphoid | 3 | 1 3.0| __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + | 70 | Basophilic Focus | | 1 | Eosinophilic Focus | X X X X X X X X X | 27 | Fatty Change | | 5 1.2| Necrosis, Focal | 2 | 13 1.8| __________________________________________________________________________|____________| Mesentery | | 2 | Fat, Necrosis | | 2 3.0| __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + | 70 | Inflammation, Chronic, Focal | | 1 2.0| Acinus, Atrophy | 3 | 2 3.5| Duct, Ectasia | 3 | 2 3.5| __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + M + + + + + + + + + + + | 69 | Inflammation, Chronic, Focal | | 9 1.8| __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + | 70 | Inflammation, Subacute | 3 | 1 3.0| __________________________________________________________________________|____________| Tongue | | 1 | Angiectasis | | 1 3.0| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + | 70 | Fibrosis | 1 | 1 1.0| Hematopoietic Cell Proliferation | | 1 4.0| Hyperplasia, Focal | 2 | 1 2.0| __________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 40 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 2| 2| | | DAY ON TEST | 4| 6| 4| 4| 6| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 3| 8| 8| | | | 1| 0| 1| 1| 0| 1| 2| 1| 1| 1| 1| 1| 1| 2| 1| 1| 1| 9| 0| 0| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | A | 500 PPM | 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + | 70 | Hyperplasia | 2 2 4 3 1 | 11 1.9| __________________________________________________________________________|____________| Parathyroid Gland | M M + M + + M + + + + + + + + + + + + + | 51 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + | 67 | Pars Distalis, Angiectasis | | 1 2.0| Pars Distalis, Hyperplasia, Focal | 2 2 3 2 3 2 | 11 2.1| __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + | 69 | Inflammation, Acute, Focal | | 1 1.0| Follicle, Cyst | | 1 | Follicular Cell, Hyperplasia, Focal | 3 | 5 2.0| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Clitoral Gland | + + + + + + + M M + + + + + + + + + | 46 | Angiectasis | | 1 3.0| __________________________________________________________________________|____________| Ovary | + + M + + + + + + + + + + + + + + + + + | 67 | Cyst | X X | 9 | __________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + | 70 | Angiectasis | 2 | 2 2.5| Fibrosis, Focal | | 1 2.0| Hydrometra | 3 2 4 4 4 | 14 3.2| Inflammation, Suppurative | | 2 4.0| Thrombosis | | 1 | Endometrium, Hyperplasia, Cystic | 2 2 3 3 3 3 4 1 2 1 3 4 3 3 2 2 | 61 2.2| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + | 70 | Angiectasis | | 1 4.0| Hyperplasia, Neutrophil | 3 | 2 3.5| Myelofibrosis | 2 2 1 3 3 2 1 4 | 21 1.8| __________________________________________________________________________|____________| Lymph Node | + | 5 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + M + + + + + + + + + + + | 68 | Lymphatic, Angiectasis | | 1 4.0| __________________________________________________________________________|____________| Lymph Node, Mesenteric | M + + + + + + + + + + + M + + + + + + + | 67 | Lymphatic, Angiectasis | 4 | 1 4.0| __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + | 70 | Congestion | | 2 2.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 41 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 2| 2| | | DAY ON TEST | 4| 6| 4| 4| 6| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 3| 8| 8| | | | 1| 0| 1| 1| 0| 1| 2| 1| 1| 1| 1| 1| 1| 2| 1| 1| 1| 9| 0| 0| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | A | 500 PPM | 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Depletion Lymphoid | | 1 1.0| Hematopoietic Cell Proliferation | 4 4 | 9 3.7| Hyperplasia, Lymphoid | | 2 2.5| __________________________________________________________________________|____________| Thymus | + + + + + + + + + + + + + + + + + + + + | 69 | Angiectasis | 3 | 1 3.0| Hyperplasia, Lymphoid | | 1 2.0| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | + + + + M + + M M + + + + + + + + + + + | 64 | Lactation | X | 2 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + | 70 | Inflammation, Chronic | 3 | 1 3.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + | 70 | Mineralization, Focal | | 1 1.0| Mineralization | 2 1 2 1 2 2 2 1 1 2 2 1 | 38 1.7| _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + | 70 | Alveolar Epithelium, Hyperplasia | | 4 1.0| __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + | 70 | Degeneration, Hyaline | 2 2 | 8 2.0| Inflammation, Chronic, Focal | 2 2 | 10 2.0| Inflammation, Chronic | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 | 56 2.0| __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Harderian Gland | + + | 5 | Hyperplasia | | 1 4.0| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + | 70 | Glomerulosclerosis | 1 | 1 1.0| Infarct | | 3 2.7| Mineralization | | 1 1.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 42 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 2| 2| | | DAY ON TEST | 4| 6| 4| 4| 6| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 3| 8| 8| | | | 1| 0| 1| 1| 0| 1| 2| 1| 1| 1| 1| 1| 1| 2| 1| 1| 1| 9| 0| 0| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | A | 500 PPM | 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ URINARY SYSTEM - cont | | | | | | Nephropathy | 2 | 1 2.0| Renal Tubule, Regeneration | | 3 1.7| __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + | 70 | Inflammation, Chronic, Focal | 2 | 9 1.8| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 43 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 7| 7| 4| 7| 4| 6| 2| 7| 6| 4| 4| 4| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 4| 4| 4| 5| 4| 5| 3| 7| 4| 7| 5| 5| 5| 4| 4| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| | 1| 1| 1| 4| 1| 5| 9| 4| 1| 9| 4| 5| 5| 1| 1| 7| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + M + M + + + + + M + + + + M + + + +| Ulcer | | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + A + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + A + + + + + + + + +| Hyperplasia, Lymphoid | 2 2 3 3 2 2 3 3 2 2 2 3| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + A + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + A + + + + + + + + +| Hyperplasia, Lymphoid | | Inflammation, Acute | | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + M + + + + + + A + + + + + + + + +| Hyperplasia, Lymphoid | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 1 1 | Basophilic Focus | | Clear Cell Focus | X| Eosinophilic Focus | X X X| Fatty Change, Focal | | Fatty Change | 1 2 1 2 | Necrosis, Focal | 1 1 | Thrombosis | | __________________________________________________________________________| Mesentery | + | Hemorrhage, Focal | 2 | Fat, Necrosis | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + A + + + + + + + + +| Foreign Body | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + A + + + + + + + + +| Inflammation, Acute | | Inflammation, Chronic | 2 | __________________________________________________________________________| Tongue | + | Hemorrhage, Focal | | Pigmentation, Focal | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Ventricle, Hypertrophy | 3 | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | | _____________________________________________________________________________________________________________________| Page 44 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 7| 7| 4| 7| 4| 6| 2| 7| 6| 4| 4| 4| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 4| 4| 4| 5| 4| 5| 3| 7| 4| 7| 5| 5| 5| 4| 4| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| | 1| 1| 1| 4| 1| 5| 9| 4| 1| 9| 4| 5| 5| 1| 1| 7| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Hyperplasia, Focal | 2 2 3 1 1 | Capsule, Hyperplasia | | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 1 2 1 2 1 | __________________________________________________________________________| Parathyroid Gland | + + + + + + + M + + + M + + M + + + + + + + + M +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + M + + + + + + + + +| Pars Distalis, Cyst | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicle, Cyst | | Follicular Cell, Hyperplasia | 1 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | | Inflammation, Chronic | 2| Spermatocele | | __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + + +| Abscess | | Cyst | X X X X X X X X X X X X X X X X| Dilatation | 3 3 2 3 4 3 4 | Hemorrhage, Focal | | Inflammation, Acute | 3 3 | Inflammation, Chronic | 3 3 4 4 3 | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Cellular | | Dilatation | 3 | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Hypospermia | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | + + + + + + + + + + + + + + + +| __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Myelofibrosis | 2 | __________________________________________________________________________| Lymph Node | + + | Pancreatic, Hyperplasia | 4 2 | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + M + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | | Hyperplasia | 2 | _____________________________________________________________________________________________________________________| Page 45 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 7| 7| 4| 7| 4| 6| 2| 7| 6| 4| 4| 4| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 4| 4| 4| 5| 4| 5| 3| 7| 4| 7| 5| 5| 5| 4| 4| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| | 1| 1| 1| 4| 1| 5| 9| 4| 1| 9| 4| 5| 5| 1| 1| 7| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Thrombosis | | __________________________________________________________________________| Spleen | + + + M + + + + + + + + + + + + + + + + + + + + +| Congestion | | Depletion Lymphoid | 1 | Fibrosis, Focal | 2 | Hematopoietic Cell Proliferation | 3 | __________________________________________________________________________| Thymus | + + + + + + M + + + + + + I + + + + + + + M + + +| Cyst | X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Mineralization, Focal | 2 | Mineralization | 3 2 2 1 2 1 2 2 2 2 3 2 2 3| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 2 | Hemorrhage, Focal | 2 2 2 | Alveolar Epithelium, Hyperplasia | | Peribronchial, Inflammation, Chronic | 1 | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2 | Inflammation, Chronic | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Harderian Gland | + | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Cyst | | Glomerulosclerosis | 4 | Hyperplasia, Lymphoid | | Infarct | | Mineralization | 1 1 1 1 1 3 | Nephropathy | | Renal Tubule, Degeneration, Granular | | _____________________________________________________________________________________________________________________| Page 46 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 7| 7| 4| 7| 4| 6| 2| 7| 6| 4| 4| 4| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 4| 4| 4| 5| 4| 5| 3| 7| 4| 7| 5| 5| 5| 4| 4| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| | 1| 1| 1| 4| 1| 5| 9| 4| 1| 9| 4| 5| 5| 1| 1| 7| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| URINARY SYSTEM - cont | | | | Renal Tubule, Regeneration | 1 2 1 1 2 2 1 1 2 2 1| __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + A + + + + + + + + +| Inflammation, Chronic, Focal | | Perivascular, Inflammation, Chronic | | _____________________________________________________________________________________________________________________| Page 47 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 2| 7| 2| 7| 4| 2| 7| 7| 2| 4| 7| 4| 7| 7| 7| 2| 7| 7| 7| 7| 2| 7| 7| 7| 4| DAY ON TEST | 7| 4| 7| 4| 5| 7| 4| 4| 7| 5| 4| 5| 4| 4| 4| 7| 4| 4| 4| 4| 7| 4| 4| 4| 5| | 5| 1| 5| 1| 5| 5| 1| 1| 5| 4| 1| 4| 1| 1| 1| 5| 1| 1| 1| 1| 4| 1| 1| 1| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + + + M + + + +| Ulcer | 3 | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + M + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | 3 4 3 3 2 2 2 2 3 4 3 2 | __________________________________________________________________________| Intestine Small, Duodenum | + + + + M + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | 4 | Inflammation, Acute | | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 1 3 | Basophilic Focus | | Clear Cell Focus | X X | Eosinophilic Focus | X X X | Fatty Change, Focal | 1 | Fatty Change | 2 2 2 3 | Necrosis, Focal | 3 | Thrombosis | | __________________________________________________________________________| Mesentery | | Hemorrhage, Focal | | Fat, Necrosis | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2 | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Foreign Body | 3 | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Acute | | Inflammation, Chronic | 2 | __________________________________________________________________________| Tongue | | Hemorrhage, Focal | | Pigmentation, Focal | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Ventricle, Hypertrophy | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | | _____________________________________________________________________________________________________________________| Page 48 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 2| 7| 2| 7| 4| 2| 7| 7| 2| 4| 7| 4| 7| 7| 7| 2| 7| 7| 7| 7| 2| 7| 7| 7| 4| DAY ON TEST | 7| 4| 7| 4| 5| 7| 4| 4| 7| 5| 4| 5| 4| 4| 4| 7| 4| 4| 4| 4| 7| 4| 4| 4| 5| | 5| 1| 5| 1| 5| 5| 1| 1| 5| 4| 1| 4| 1| 1| 1| 5| 1| 1| 1| 1| 4| 1| 1| 1| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Hyperplasia, Focal | 2 2 1 2 | Capsule, Hyperplasia | | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 2 1 4 2 2 2 1 2 4 3 | __________________________________________________________________________| Parathyroid Gland | + + M + I M M + M + + + + + + + + + + + + M + M +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + M + + + + + + + +| Pars Distalis, Cyst | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicle, Cyst | | Follicular Cell, Hyperplasia | 1 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | | Inflammation, Chronic | | Spermatocele | | __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + +| Abscess | | Cyst | X X X X X X X X X X X | Dilatation | 3 3 3 3 3 3 4 4 3 4 4 3| Hemorrhage, Focal | 2 | Inflammation, Acute | 4 4 4 | Inflammation, Chronic | 2 | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + M| __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Cellular | 2 | Dilatation | 2 4 4 | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Hypospermia | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | + | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Myelofibrosis | | __________________________________________________________________________| Lymph Node | | Pancreatic, Hyperplasia | | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + M + + + + + + +| Congestion | 2 2 | Hyperplasia | | _____________________________________________________________________________________________________________________| Page 49 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 2| 7| 2| 7| 4| 2| 7| 7| 2| 4| 7| 4| 7| 7| 7| 2| 7| 7| 7| 7| 2| 7| 7| 7| 4| DAY ON TEST | 7| 4| 7| 4| 5| 7| 4| 4| 7| 5| 4| 5| 4| 4| 4| 7| 4| 4| 4| 4| 7| 4| 4| 4| 5| | 5| 1| 5| 1| 5| 5| 1| 1| 5| 4| 1| 4| 1| 1| 1| 5| 1| 1| 1| 1| 4| 1| 1| 1| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Thrombosis | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | | Depletion Lymphoid | 1 1 1 | Fibrosis, Focal | 3 | Hematopoietic Cell Proliferation | 4 3 3 | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + + M + M + + + M +| Cyst | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Mineralization, Focal | 1 2 2 | Mineralization | 3 3 2 2 2 3 3 1 2 2 3 2 2| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Hemorrhage, Focal | | Alveolar Epithelium, Hyperplasia | 1 | Peribronchial, Inflammation, Chronic | 1 1 1 | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2 2 1 2 2 2 | Inflammation, Chronic | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Harderian Gland | + | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Cyst | | Glomerulosclerosis | | Hyperplasia, Lymphoid | 3 2 | Infarct | 2 | Mineralization | 1 1 1 1 2 1 1 | Nephropathy | | Renal Tubule, Degeneration, Granular | | _____________________________________________________________________________________________________________________| Page 50 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 2| 7| 2| 7| 4| 2| 7| 7| 2| 4| 7| 4| 7| 7| 7| 2| 7| 7| 7| 7| 2| 7| 7| 7| 4| DAY ON TEST | 7| 4| 7| 4| 5| 7| 4| 4| 7| 5| 4| 5| 4| 4| 4| 7| 4| 4| 4| 4| 7| 4| 4| 4| 5| | 5| 1| 5| 1| 5| 5| 1| 1| 5| 4| 1| 4| 1| 1| 1| 5| 1| 1| 1| 1| 4| 1| 1| 1| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| URINARY SYSTEM - cont | | | | Renal Tubule, Regeneration | 1 2 1 1 1 2 2 1 2 1 1 2 1 1| __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 1 | Perivascular, Inflammation, Chronic | 2 | _____________________________________________________________________________________________________________________| Page 51 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 7| 4| 7| 7| 7| 2| 7| 7| 7| 6| 7| 7| 7| 7| 2| 7| 6| 7| 7| 2| | | DAY ON TEST | 4| 5| 4| 4| 4| 7| 4| 4| 4| 1| 4| 4| 4| 4| 7| 4| 4| 4| 4| 7| | | | 1| 4| 1| 1| 1| 4| 1| 1| 1| 7| 1| 1| 1| 1| 5| 1| 0| 1| 1| 4| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 0 PPM | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | M + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + A + + + | 64 | Ulcer | | 1 3.0| __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + A + + + | 67 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + A + + + | 69 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + A + + + | 68 | Hyperplasia, Lymphoid | 3 3 3 2 2 2 1 2 3 | 33 2.5| __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + A + + + | 67 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + A + + + | 68 | Hyperplasia, Lymphoid | 4 4 | 3 4.0| Inflammation, Acute | 4 | 1 4.0| __________________________________________________________________________|____________| Intestine Small, Ileum | + M + + + + + + + + + + + + + + A + + + | 66 | Hyperplasia, Lymphoid | 4 | 1 4.0| __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + | 70 | Angiectasis | | 4 1.5| Basophilic Focus | X | 1 | Clear Cell Focus | X X | 5 | Eosinophilic Focus | | 6 | Fatty Change, Focal | 1 | 2 1.0| Fatty Change | 1 2 1 2 | 12 1.8| Necrosis, Focal | | 3 1.7| Thrombosis | X | 1 | __________________________________________________________________________|____________| Mesentery | + | 2 | Hemorrhage, Focal | | 1 2.0| Fat, Necrosis | 2 | 1 2.0| __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + | 70 | Inflammation, Chronic, Focal | 1 | 2 1.5| __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + | 69 | Foreign Body | | 1 3.0| __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + A + + + | 68 | Inflammation, Acute | 3 | 1 3.0| Inflammation, Chronic | | 2 2.0| __________________________________________________________________________|____________| Tongue | + + | 3 | Hemorrhage, Focal | 4 | 1 4.0| Pigmentation, Focal | 2 | 1 2.0| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 52 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 7| 4| 7| 7| 7| 2| 7| 7| 7| 6| 7| 7| 7| 7| 2| 7| 6| 7| 7| 2| | | DAY ON TEST | 4| 5| 4| 4| 4| 7| 4| 4| 4| 1| 4| 4| 4| 4| 7| 4| 4| 4| 4| 7| | | | 1| 4| 1| 1| 1| 4| 1| 1| 1| 7| 1| 1| 1| 1| 5| 1| 0| 1| 1| 4| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 0 PPM | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | __________________________________________________________________________________________________________________________________ CARDIOVASCULAR SYSTEM - cont | | | | | | Ventricle, Hypertrophy | | 1 3.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + M + + + + + + + + + + | 69 | Atrophy | 2 2 | 2 2.0| Hyperplasia, Focal | 3 2 2 2 2 | 14 1.9| Capsule, Hyperplasia | 2 | 1 2.0| __________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + M + + + + + + M + + + | 68 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + | 70 | Hyperplasia | 1 3 1 3 1 1 1 2 | 25 2.0| __________________________________________________________________________|____________| Parathyroid Gland | M M M + + + + + M + M + + + + + M M + + | 52 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + | 68 | Pars Distalis, Cyst | X | 1 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + | 70 | Follicle, Cyst | X | 1 | Follicular Cell, Hyperplasia | | 2 1.0| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + | 70 | Atrophy | 2 | 1 2.0| Inflammation, Chronic | | 1 2.0| Spermatocele | 3 | 1 3.0| __________________________________________________________________________|____________| Preputial Gland | + + + + + + + + + + + + + + + + + + | 54 | Abscess | 4 | 1 4.0| Cyst | X X X X X X X X X | 36 | Dilatation | 4 3 4 4 4 3 3 3 2 | 28 3.3| Hemorrhage, Focal | | 1 2.0| Inflammation, Acute | 4 | 6 3.7| Inflammation, Chronic | 4 3 4 | 9 3.3| __________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + | 70 | Depletion Cellular | 1 2 2 | 4 1.8| Dilatation | 3 | 5 3.2| __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + | 70 | Hypospermia | 2 | 1 2.0| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 53 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 7| 4| 7| 7| 7| 2| 7| 7| 7| 6| 7| 7| 7| 7| 2| 7| 6| 7| 7| 2| | | DAY ON TEST | 4| 5| 4| 4| 4| 7| 4| 4| 4| 1| 4| 4| 4| 4| 7| 4| 4| 4| 4| 7| | | | 1| 4| 1| 1| 1| 4| 1| 1| 1| 7| 1| 1| 1| 1| 5| 1| 0| 1| 1| 4| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 0 PPM | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | | | | __________________________________________________________________________|____________| Blood | | 17 | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + | 70 | Myelofibrosis | | 1 2.0| __________________________________________________________________________|____________| Lymph Node | + | 3 | Pancreatic, Hyperplasia | | 2 3.0| __________________________________________________________________________|____________| Lymph Node, Mandibular | + M + + + + + + + M + + + + + + + + + M | 66 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + M + + + | 68 | Congestion | | 2 2.0| Hyperplasia | | 1 2.0| Thrombosis | 4 | 1 4.0| __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + | 69 | Congestion | 2 | 1 2.0| Depletion Lymphoid | 3 | 5 1.4| Fibrosis, Focal | | 2 2.5| Hematopoietic Cell Proliferation | 4 4 | 6 3.5| __________________________________________________________________________|____________| Thymus | M + + + M + + M + + + M + + + M M + + + | 58 | Cyst | | 1 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M | | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + | 70 | Mineralization, Focal | 2 | 5 1.8| Mineralization | 3 2 2 2 2 2 2 3 2 | 36 2.2| _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + | 70 | Angiectasis | | 1 2.0| Hemorrhage, Focal | 2 2 | 5 2.0| Alveolar Epithelium, Hyperplasia | 2 1 | 3 1.3| Peribronchial, Inflammation, Chronic | | 4 1.0| __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + | 70 | Inflammation, Chronic, Focal | 2 2 2 2 | 11 1.9| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 54 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 7| 4| 7| 7| 7| 2| 7| 7| 7| 6| 7| 7| 7| 7| 2| 7| 6| 7| 7| 2| | | DAY ON TEST | 4| 5| 4| 4| 4| 7| 4| 4| 4| 1| 4| 4| 4| 4| 7| 4| 4| 4| 4| 7| | | | 1| 4| 1| 1| 1| 4| 1| 1| 1| 7| 1| 1| 1| 1| 5| 1| 0| 1| 1| 4| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 0 PPM | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | __________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - cont | | | | | | Inflammation, Chronic | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 | 55 2.0| __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Harderian Gland | + + + | 5 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + | 70 | Cyst | X | 1 | Glomerulosclerosis | | 1 4.0| Hyperplasia, Lymphoid | | 2 2.5| Infarct | | 1 2.0| Mineralization | 1 2 2 | 16 1.3| Nephropathy | 2 | 1 2.0| Renal Tubule, Degeneration, Granular | 4 | 1 4.0| Renal Tubule, Regeneration | 3 2 1 1 2 1 2 1 1 1 1 2 1 | 38 1.4| __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + A + + + | 68 | Inflammation, Chronic, Focal | | 1 1.0| Perivascular, Inflammation, Chronic | | 1 2.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 55 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 4| 7| 7| 7| 2| 6| 4| 7| 7| 4| 6| 7| 7| 7| 7| 4| 7| 7| 7| 7| 4| 4| 2| 7| 7| DAY ON TEST | 5| 4| 4| 4| 7| 2| 5| 4| 0| 5| 7| 4| 4| 4| 4| 5| 4| 4| 4| 4| 5| 5| 7| 4| 4| | 5| 0| 0| 0| 5| 8| 5| 0| 7| 5| 4| 0| 0| 0| 0| 4| 0| 0| 0| 0| 5| 5| 5| 0| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 50 PPM | 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + A + + M + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + A + + + + + + + + + M + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + A + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + A + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | 3 4 3 2 3 3 4 2 3 | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + A + + A + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + A + + A + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | | Ulcer | | __________________________________________________________________________| Intestine Small, Ileum | + + + + + A + + A + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 1 | Basophilic Focus | X | Clear Cell Focus | | Eosinophilic Focus | X X X | Fatty Change | 3 2 2 | Inflammation, Chronic, Focal | | Necrosis, Focal | | __________________________________________________________________________| Mesentery | + | Fat, Necrosis | 4 | __________________________________________________________________________| Pancreas | + + + + + A + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2 1 | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Glandular | + + + + + A + + + + + + + + + + + + + + + + + + +| Erosion | 2 | Inflammation, Chronic | | Mucosa, Hyperplasia, Focal | 3 | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Mineralization | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + A + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | 3 2 2 2 2 2 | Capsule, Hyperplasia, Focal | 3 | __________________________________________________________________________| Adrenal Medulla | + M + + + A + + + + + + + + + + + + + + + + M + +| Hyperplasia | 3 | __________________________________________________________________________| Islets, Pancreatic | + + + + + A + + + + + + + + + + + + + + + + + + +| Hyperplasia | 1 2 2 3 3 2 2 3 2 4| __________________________________________________________________________| Parathyroid Gland | M + + M M M + + + M M + M + + + + + + + I + + + M| _____________________________________________________________________________________________________________________| Page 56 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 4| 7| 7| 7| 2| 6| 4| 7| 7| 4| 6| 7| 7| 7| 7| 4| 7| 7| 7| 7| 4| 4| 2| 7| 7| DAY ON TEST | 5| 4| 4| 4| 7| 2| 5| 4| 0| 5| 7| 4| 4| 4| 4| 5| 4| 4| 4| 4| 5| 5| 7| 4| 4| | 5| 0| 0| 0| 5| 8| 5| 0| 7| 5| 4| 0| 0| 0| 0| 4| 0| 0| 0| 0| 5| 5| 5| 0| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 50 PPM | 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Pituitary Gland | + + + + + M + + + + + + + + + + + + + M + + + + +| __________________________________________________________________________| Thyroid Gland | + + + + + + + + + M + + + + + + + + + + + + + + +| Follicular Cell, Hyperplasia | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Spermatocele | | __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + +| Cyst | X X X X X X X X X X X X X X X| Dilatation | 3 2 3 3 2 4 3 | Inflammation, Acute | 3 4 4 4 | Inflammation, Chronic | 4 1 3 1 2| __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic | | __________________________________________________________________________| Seminal Vesicle | + + + + + A + + + + + + + + + + + + + + + + + + +| Depletion Cellular | 3 2 | Dilatation | 3 4| __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Hypospermia | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Neutrophil | | __________________________________________________________________________| Lymph Node | + + | Mediastinal, Infiltration Cellular, | | Plasma Cell | | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + M + + + + + + + I + + + M + M + + + +| __________________________________________________________________________| Lymph Node, Mesenteric | + + M + + A + + + + + + + + + + + + + + + + + + +| Congestion | 2 | Hyperplasia, Lymphoid | 2 | __________________________________________________________________________| Spleen | + + + + + A + + + + + + + + + + + + + + + + + + +| Congestion | | Cyst | | Depletion Lymphoid | | Hematopoietic Cell Proliferation | 3 4 | Hyperplasia, Lymphoid | 3 | __________________________________________________________________________| Thymus | + + + + + A + + + + M + + + + M + + + + + + + + +| Hyperplasia, Lymphoid | 2 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| _____________________________________________________________________________________________________________________| Page 57 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 4| 7| 7| 7| 2| 6| 4| 7| 7| 4| 6| 7| 7| 7| 7| 4| 7| 7| 7| 7| 4| 4| 2| 7| 7| DAY ON TEST | 5| 4| 4| 4| 7| 2| 5| 4| 0| 5| 7| 4| 4| 4| 4| 5| 4| 4| 4| 4| 5| 5| 7| 4| 4| | 5| 0| 0| 0| 5| 8| 5| 0| 7| 5| 4| 0| 0| 0| 0| 4| 0| 0| 0| 0| 5| 5| 5| 0| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 50 PPM | 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM - cont | | | | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperostosis | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Mineralization, Focal | 2 | Mineralization | 2 3 3 2 2 2 2 3 2 3 3 3 2 2 2 2| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + M + + + + + + + + +| Congestion | | Hemorrhage, Focal | | Infiltration Cellular, Histiocyte | | Inflammation, Chronic | | Alveolar Epithelium, Hyperplasia | | Peribronchial, Inflammation, Chronic | 1 | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2 | Inflammation, Chronic | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2| Metaplasia, Squamous | 2 | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | | __________________________________________________________________________| Harderian Gland | + | Hyperplasia | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Cyst | | Glomerulosclerosis | 2 | Hemorrhage | | Infarct | | Inflammation, Chronic, Focal | 1 | Mineralization | 2 3 2 1 1 1 1 1| Renal Tubule, Regeneration | 2 2 3 2 1 2 1 2 1 2 2 1 2| __________________________________________________________________________| Urinary Bladder | + + + + + A + + A + + + + + + + + + + + + + + + +| Calculus Micro Observation Only | | Inflammation, Chronic, Focal | 1 | _____________________________________________________________________________________________________________________| Page 58 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 2| 2| DAY ON TEST | 7| 7| 7| 7| 7| 4| 4| 4| 5| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 0| 4| 5| 4| 7| 7| | 4| 4| 4| 4| 4| 0| 0| 0| 5| 0| 0| 1| 1| 1| 1| 1| 1| 1| 0| 8| 0| 4| 0| 4| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 50 PPM | 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + M + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | 2 4 2 3 4 | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | | Ulcer | 3 | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Basophilic Focus | X | Clear Cell Focus | X | Eosinophilic Focus | X X | Fatty Change | 2 2 2 2 1 2 2 | Inflammation, Chronic, Focal | | Necrosis, Focal | | __________________________________________________________________________| Mesentery | | Fat, Necrosis | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | 3 2 | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2 1 2 | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Erosion | | Inflammation, Chronic | 2 | Mucosa, Hyperplasia, Focal | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Mineralization | 4 | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | 2 2 2 2 | Capsule, Hyperplasia, Focal | | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 1 2 3 3 1 2 3 2 3 3 | __________________________________________________________________________| Parathyroid Gland | + M + + M + + + M + + + + + + + + + + + M + + M M| _____________________________________________________________________________________________________________________| Page 59 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 2| 2| DAY ON TEST | 7| 7| 7| 7| 7| 4| 4| 4| 5| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 0| 4| 5| 4| 7| 7| | 4| 4| 4| 4| 4| 0| 0| 0| 5| 0| 0| 1| 1| 1| 1| 1| 1| 1| 0| 8| 0| 4| 0| 4| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 50 PPM | 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicular Cell, Hyperplasia | 2 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Spermatocele | | __________________________________________________________________________| Preputial Gland | + + + + M + + + + + + + + + + + + + + | Cyst | X X X X X X X X X X X X X X | Dilatation | 4 4 2 3 4 3 4 3 2 | Inflammation, Acute | 4 4 | Inflammation, Chronic | 2 4 | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + M +| Inflammation, Chronic | 1 | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Cellular | | Dilatation | 4 | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Hypospermia | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Neutrophil | 4 2 | __________________________________________________________________________| Lymph Node | + | Mediastinal, Infiltration Cellular, | | Plasma Cell | 3 | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mesenteric | M + + + + + + + + + + + + + + + + + + + + M + + +| Congestion | 4 3 | Hyperplasia, Lymphoid | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | 3 | Cyst | X | Depletion Lymphoid | | Hematopoietic Cell Proliferation | 4 2 2 3 | Hyperplasia, Lymphoid | | __________________________________________________________________________| Thymus | + + + + + + + + + + + + M M + M + + + + + + + + +| Hyperplasia, Lymphoid | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| _____________________________________________________________________________________________________________________| Page 60 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 2| 2| DAY ON TEST | 7| 7| 7| 7| 7| 4| 4| 4| 5| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 0| 4| 5| 4| 7| 7| | 4| 4| 4| 4| 4| 0| 0| 0| 5| 0| 0| 1| 1| 1| 1| 1| 1| 1| 0| 8| 0| 4| 0| 4| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 50 PPM | 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM - cont | | | | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperostosis | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Mineralization, Focal | 2 2 | Mineralization | 2 3 2 2 2 2 2 1 2 2 1 1 | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | 2| Hemorrhage, Focal | | Infiltration Cellular, Histiocyte | 4 | Inflammation, Chronic | 3 | Alveolar Epithelium, Hyperplasia | | Peribronchial, Inflammation, Chronic | 1 1 1| __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2 2 1 2 2 2| Inflammation, Chronic | 2 1 1 2 2 2 2 2 2 2 2 2 2 2 2 | Metaplasia, Squamous | | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | + | __________________________________________________________________________| Harderian Gland | + | Hyperplasia | 4 | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Cyst | | Glomerulosclerosis | | Hemorrhage | | Infarct | | Inflammation, Chronic, Focal | 2 | Mineralization | 1 1 | Renal Tubule, Regeneration | 1 1 1 2 3 2 2 2 2 2 1 1 2 | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Calculus Micro Observation Only | X | Inflammation, Chronic, Focal | 2 1 1 1| _____________________________________________________________________________________________________________________| Page 61 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 4| 4| 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 4| 4| 4| 4| 4| 5| 5| 2| 7| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | | 1| 1| 1| 1| 1| 4| 5| 0| 4| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 50 PPM | 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + + + + + | 67 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + M + + + + + + + + + + + + + + | 67 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + | 69 | Hyperplasia, Lymphoid | 2 2 2 2 | 18 2.8| __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + | 68 | Hyperplasia, Lymphoid | 4 | 1 4.0| Ulcer | | 1 3.0| __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + | 70 | Angiectasis | 2 | 2 1.5| Basophilic Focus | | 2 | Clear Cell Focus | X X | 3 | Eosinophilic Focus | X X X X | 9 | Fatty Change | 2 1 2 | 13 1.9| Inflammation, Chronic, Focal | 3 | 1 3.0| Necrosis, Focal | 2 | 1 2.0| __________________________________________________________________________|____________| Mesentery | + + | 3 | Fat, Necrosis | 2 | 2 3.0| __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + | 69 | Hyperplasia, Focal | | 2 2.5| __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + | 70 | Inflammation, Chronic, Focal | 2 3 | 7 1.9| __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + | 69 | Erosion | | 1 2.0| Inflammation, Chronic | | 1 2.0| Mucosa, Hyperplasia, Focal | | 1 3.0| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + | 70 | Mineralization | | 1 4.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + | 69 | Hyperplasia, Focal | 2 2 2 3 2 2 2 2 | 18 2.1| Capsule, Hyperplasia, Focal | | 1 3.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 62 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 4| 4| 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 4| 4| 4| 4| 4| 5| 5| 2| 7| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | | 1| 1| 1| 1| 1| 4| 5| 0| 4| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 50 PPM | 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + | 67 | Hyperplasia | | 1 3.0| __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + M + + + + + + + + + + + + | 68 | Hyperplasia | 3 3 2 3 2 3 2 | 27 2.4| __________________________________________________________________________|____________| Parathyroid Gland | + + + M M + M M M + M + + + + + + + M M | 47 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + | 69 | Follicular Cell, Hyperplasia | | 1 2.0| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + | 70 | Spermatocele | X | 1 | __________________________________________________________________________|____________| Preputial Gland | + + + + + + + + + + + + + + + + + | 53 | Cyst | X X X X X X X X X X X X X | 42 | Dilatation | 2 4 4 2 4 4 4 4 4 | 25 3.2| Inflammation, Acute | 3 3 | 8 3.6| Inflammation, Chronic | 3 | 8 2.5| __________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + + + + + + | 69 | Inflammation, Chronic | | 1 1.0| __________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + | 69 | Depletion Cellular | | 2 2.5| Dilatation | 4 4 | 5 3.8| __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + | 70 | Hypospermia | 3 | 1 3.0| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + | 70 | Hyperplasia, Neutrophil | | 2 3.0| __________________________________________________________________________|____________| Lymph Node | + + | 5 | Mediastinal, Infiltration Cellular, | | | Plasma Cell | | 1 3.0| __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + M + + + + + + + + + + + + | 65 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + M + + + + + + + + + + + + + | 65 | Congestion | | 3 3.0| Hyperplasia, Lymphoid | | 1 2.0| __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 63 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 4| 4| 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 4| 4| 4| 4| 4| 5| 5| 2| 7| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | | 1| 1| 1| 1| 1| 4| 5| 0| 4| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 50 PPM | 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Congestion | | 1 3.0| Cyst | | 1 | Depletion Lymphoid | 1 1 1 | 3 1.0| Hematopoietic Cell Proliferation | 2 | 7 2.9| Hyperplasia, Lymphoid | | 1 3.0| __________________________________________________________________________|____________| Thymus | + + + + + + + M + + + M + + + M + + M + | 60 | Hyperplasia, Lymphoid | | 1 2.0| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M M M M M M M + M M M M M M M M M M M M | 1 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + | 70 | Hyperostosis | X | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + | 70 | Mineralization, Focal | | 3 2.0| Mineralization | 2 3 1 1 3 2 2 1 2 3 2 2 3 3 | 42 2.1| _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + | 69 | Congestion | | 1 2.0| Hemorrhage, Focal | 2 | 1 2.0| Infiltration Cellular, Histiocyte | | 1 4.0| Inflammation, Chronic | | 1 3.0| Alveolar Epithelium, Hyperplasia | 1 | 1 1.0| Peribronchial, Inflammation, Chronic | | 4 1.0| __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + | 70 | Inflammation, Chronic, Focal | 2 | 8 1.9| Inflammation, Chronic | 2 2 2 2 2 2 2 2 2 2 2 2 2 1 3 2 2 | 53 2.0| Metaplasia, Squamous | | 1 2.0| __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Ear | | 1 | __________________________________________________________________________|____________| Harderian Gland | | 2 | Hyperplasia | | 1 4.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 64 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 4| 4| 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 4| 4| 4| 4| 4| 5| 5| 2| 7| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | | 1| 1| 1| 1| 1| 4| 5| 0| 4| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 50 PPM | 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | __________________________________________________________________________________________________________________________________ SPECIAL SENSES SYSTEM - cont | | | | | | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + | 70 | Cyst | X | 1 | Glomerulosclerosis | | 1 2.0| Hemorrhage | 3 | 1 3.0| Infarct | 4 3 | 2 3.5| Inflammation, Chronic, Focal | | 2 1.5| Mineralization | 1 1 2 1 | 14 1.4| Renal Tubule, Regeneration | 2 2 3 2 1 2 2 2 3 1 2 | 37 1.8| __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + | 68 | Calculus Micro Observation Only | | 1 | Inflammation, Chronic, Focal | 1 | 6 1.2| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 65 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 2| 2| 2| 7| 7| 7| 2| 7| 4| 7| 7| 7| 7| 5| 2| 4| 2| 7| 7| 4| 7| 4| 4| 7| DAY ON TEST | 3| 7| 7| 7| 3| 3| 3| 7| 3| 5| 3| 3| 3| 3| 8| 7| 5| 7| 3| 3| 5| 3| 5| 5| 3| | 7| 4| 5| 4| 7| 7| 7| 4| 7| 5| 7| 7| 7| 7| 5| 5| 5| 4| 6| 7| 4| 6| 4| 5| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 250 PPM | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + M M + M + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | 2 2 3 3 2 3 3| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + M + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Basophilic Focus | X | Clear Cell Focus | X X | Eosinophilic Focus | X X | Fatty Change | 1 1 2 2 1 1 2 | Inflammation, Chronic, Focal | 2 | Necrosis, Focal | 1 1 | Thrombosis | | __________________________________________________________________________| Mesentery | | Cyst | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 1 2 | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperkeratosis, Focal | 4 | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Erosion | | Inflammation, Chronic | | Perivascular, Inflammation, Chronic | 1 | __________________________________________________________________________| Tongue | + | Hyperkeratosis, Focal | 3 | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | 2 2 2 2 2 | Capsule, Hyperplasia | | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 1 1 1 1 1 1 | _____________________________________________________________________________________________________________________| Page 66 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 2| 2| 2| 7| 7| 7| 2| 7| 4| 7| 7| 7| 7| 5| 2| 4| 2| 7| 7| 4| 7| 4| 4| 7| DAY ON TEST | 3| 7| 7| 7| 3| 3| 3| 7| 3| 5| 3| 3| 3| 3| 8| 7| 5| 7| 3| 3| 5| 3| 5| 5| 3| | 7| 4| 5| 4| 7| 7| 7| 4| 7| 5| 7| 7| 7| 7| 5| 5| 5| 4| 6| 7| 4| 6| 4| 5| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 250 PPM | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Parathyroid Gland | + + + M + + + M + + + + + M + + + M + M + M + + M| __________________________________________________________________________| Pituitary Gland | + + + + M + M + + + M + + M + + + + M + + + + + +| __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | 2 | __________________________________________________________________________| Penis | | __________________________________________________________________________| Preputial Gland | + + + + + M + + + + + + + + + + +| Abscess | | Atrophy | 2 | Cyst | X X X X X X X X X X X X X| Dilatation | 3 3 4 4 3 4 3 4| Inflammation, Acute | | Inflammation, Chronic | 2 3 4 | __________________________________________________________________________| Prostate | + + + M + + + + + + + + + + + + + + + + + + + + +| Atrophy | 2 3 | Inflammation, Chronic, Focal | 1 | Inflammation, Chronic | 1 1 | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Cellular | 3 2 3 | Dilatation | 4 3 | Fibrosis | | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Hypospermia | 4 | Seminiferous Tubule, Atrophy | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Neutrophil | | Myelofibrosis | 2 | __________________________________________________________________________| Lymph Node | | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + M + + + + + + + + + + + + + + I + +| __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + M + + + + + + + + + + + + +| Congestion | 4 | Hyperplasia | | __________________________________________________________________________| Spleen | + + + + + + + + + + + M + + + + + + + + + + + + +| Congestion | 2 | Depletion Lymphoid | 2 1 2 | Hematopoietic Cell Proliferation | 4 3 | __________________________________________________________________________| Thymus | + + + + + + + + M + + + M + + + + + + + + + + + M| _____________________________________________________________________________________________________________________| Page 67 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 2| 2| 2| 7| 7| 7| 2| 7| 4| 7| 7| 7| 7| 5| 2| 4| 2| 7| 7| 4| 7| 4| 4| 7| DAY ON TEST | 3| 7| 7| 7| 3| 3| 3| 7| 3| 5| 3| 3| 3| 3| 8| 7| 5| 7| 3| 3| 5| 3| 5| 5| 3| | 7| 4| 5| 4| 7| 7| 7| 4| 7| 5| 7| 7| 7| 7| 5| 5| 5| 4| 6| 7| 4| 6| 4| 5| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 250 PPM | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + I + + + + + + + +| Ulcer | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Mineralization, Focal | 1 2 1 1 | Mineralization | 2 3 1 2 2 2 2 3 2 2 2 2 | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | 2 | Hemorrhage, Focal | | Alveolar Epithelium, Hyperplasia | 2 | Peribronchial, Inflammation, Chronic | 1 2 1 2 1 | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Degeneration, Hyaline | 2 2 | Inflammation, Chronic, Focal | 2 2 2 2 2 2 | Inflammation, Chronic | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2| Metaplasia, Squamous | | Olfactory Epithelium, Hyperplasia | | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Cyst | | Glomerulosclerosis | | Hyperplasia, Lymphoid | | Infarct | | Metaplasia, Osseous | 3| Mineralization | 1 1 1 1 1| Nephropathy | 2 | Renal Tubule, Regeneration | 1 2 1 1 1 3 1 1 2 2 3| __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Concretion | 2 | Ectasia | | Inflammation, Chronic, Focal | 1 | _____________________________________________________________________________________________________________________| Page 68 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 5| 7| 7| 7| 7| 4| 7| 4| 7| 7| 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 2| 7| 7| 4| DAY ON TEST | 3| 4| 3| 3| 4| 4| 5| 3| 5| 4| 4| 4| 4| 4| 4| 5| 4| 4| 4| 4| 4| 7| 4| 4| 5| | 6| 1| 6| 6| 0| 0| 4| 1| 4| 0| 0| 0| 0| 0| 0| 5| 0| 0| 0| 0| 0| 5| 0| 0| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 250 PPM | 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | 4 3 3 3 2 3 3 3 | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | 4 1 | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 2 | Basophilic Focus | X X X| Clear Cell Focus | X | Eosinophilic Focus | X X X | Fatty Change | 2 3 | Inflammation, Chronic, Focal | | Necrosis, Focal | 3 | Thrombosis | X | __________________________________________________________________________| Mesentery | + | Cyst | X | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + M + + +| __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 1 1 | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperkeratosis, Focal | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Erosion | 2 | Inflammation, Chronic | | Perivascular, Inflammation, Chronic | | __________________________________________________________________________| Tongue | | Hyperkeratosis, Focal | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | 3 2 2 2 | Capsule, Hyperplasia | 2 | __________________________________________________________________________| Adrenal Medulla | + + M + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | 2 | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + M + + +| Hyperplasia | 1 2 1 2 1 1 2 | _____________________________________________________________________________________________________________________| Page 69 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 5| 7| 7| 7| 7| 4| 7| 4| 7| 7| 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 2| 7| 7| 4| DAY ON TEST | 3| 4| 3| 3| 4| 4| 5| 3| 5| 4| 4| 4| 4| 4| 4| 5| 4| 4| 4| 4| 4| 7| 4| 4| 5| | 6| 1| 6| 6| 0| 0| 4| 1| 4| 0| 0| 0| 0| 0| 0| 5| 0| 0| 0| 0| 0| 5| 0| 0| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 250 PPM | 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Parathyroid Gland | M + + + + + M M M + + M + M + + + + + + M I M + +| __________________________________________________________________________| Pituitary Gland | + + M + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | | __________________________________________________________________________| Penis | | __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + | Abscess | 3 | Atrophy | | Cyst | X X X X X X X X X X X X X X X X X X | Dilatation | 3 1 3 4 2 2 2 2 2 | Inflammation, Acute | 4 4 4 2 4 4 | Inflammation, Chronic | 4 2 2 2 3 | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | | Inflammation, Chronic, Focal | | Inflammation, Chronic | | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Cellular | | Dilatation | 4 4 | Fibrosis | 4 | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Hypospermia | | Seminiferous Tubule, Atrophy | 3 | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Neutrophil | | Myelofibrosis | | __________________________________________________________________________| Lymph Node | + | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + M + +| Congestion | 3 2 | Hyperplasia | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | | Depletion Lymphoid | | Hematopoietic Cell Proliferation | 3 4 3 | __________________________________________________________________________| Thymus | + M M + + + + M + + M + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 70 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 7| 5| 7| 7| 7| 7| 4| 7| 4| 7| 7| 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 2| 7| 7| 4| DAY ON TEST | 3| 4| 3| 3| 4| 4| 5| 3| 5| 4| 4| 4| 4| 4| 4| 5| 4| 4| 4| 4| 4| 7| 4| 4| 5| | 6| 1| 6| 6| 0| 0| 4| 1| 4| 0| 0| 0| 0| 0| 0| 5| 0| 0| 0| 0| 0| 5| 0| 0| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 250 PPM | 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Ulcer | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Mineralization, Focal | | Mineralization | 3 2 2 3 1 2 2 3 2 1 | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | | Hemorrhage, Focal | | Alveolar Epithelium, Hyperplasia | 1 | Peribronchial, Inflammation, Chronic | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Degeneration, Hyaline | | Inflammation, Chronic, Focal | 2 | Inflammation, Chronic | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2| Metaplasia, Squamous | | Olfactory Epithelium, Hyperplasia | 2 | __________________________________________________________________________| Trachea | + + + + + + + M + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Cyst | X | Glomerulosclerosis | | Hyperplasia, Lymphoid | 3 | Infarct | 2 | Metaplasia, Osseous | | Mineralization | 2 1 1 1 1 1 | Nephropathy | | Renal Tubule, Regeneration | 2 1 1 1 1 1 1 2 1 1 2 1 3 2 1 2 | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Concretion | | Ectasia | | Inflammation, Chronic, Focal | 1| _____________________________________________________________________________________________________________________| Page 71 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 4| 7| 2| 7| 7| 7| 7| 7| 7| 7| 6| 2| 7| 6| 7| 2| 7| 7| 6| 7| | | DAY ON TEST | 5| 4| 7| 4| 4| 4| 4| 4| 4| 4| 0| 7| 4| 2| 4| 7| 1| 4| 1| 4| | | | 4| 0| 5| 0| 0| 0| 0| 0| 0| 0| 1| 4| 0| 6| 0| 4| 0| 0| 0| 0| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 250 PPM | 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + M + + + + + + + + + | 69 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + A + + M + + + + M + | 64 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + A + + + + + A + + + | 68 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + A + + + + + A + + + | 68 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + A + + + + + A + + + | 68 | Hyperplasia, Lymphoid | 3 3 3 3 4 2 2 3 | 23 2.8| __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + A + + + + + A + + + | 68 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + A + + + + + A + + M | 67 | Hyperplasia, Lymphoid | | 2 2.5| __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + A + + + + + A + + + | 67 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + | 70 | Angiectasis | | 1 2.0| Basophilic Focus | X | 5 | Clear Cell Focus | | 3 | Eosinophilic Focus | X X X X | 9 | Fatty Change | 2 3 2 2 | 13 1.8| Inflammation, Chronic, Focal | | 1 2.0| Necrosis, Focal | 1 | 4 1.5| Thrombosis | | 1 | __________________________________________________________________________|____________| Mesentery | | 1 | Cyst | | 1 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + | 70 | Inflammation, Chronic, Focal | | 4 1.3| __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + | 70 | Hyperkeratosis, Focal | | 1 4.0| __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + | 70 | Erosion | 2 | 2 2.0| Inflammation, Chronic | 2 | 1 2.0| Perivascular, Inflammation, Chronic | | 1 1.0| __________________________________________________________________________|____________| Tongue | | 1 | Hyperkeratosis, Focal | | 1 3.0| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + | 70 | Hyperplasia, Focal | 2 2 1 1 2 2 | 15 1.9| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 72 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 4| 7| 2| 7| 7| 7| 7| 7| 7| 7| 6| 2| 7| 6| 7| 2| 7| 7| 6| 7| | | DAY ON TEST | 5| 4| 7| 4| 4| 4| 4| 4| 4| 4| 0| 7| 4| 2| 4| 7| 1| 4| 1| 4| | | | 4| 0| 5| 0| 0| 0| 0| 0| 0| 0| 1| 4| 0| 6| 0| 4| 0| 0| 0| 0| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 250 PPM | 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | Capsule, Hyperplasia | | 1 2.0| __________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + | 69 | Hyperplasia, Focal | 2 | 2 2.0| __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + | 69 | Hyperplasia | 1 2 2 1 3 1 4 2 2 | 22 1.5| __________________________________________________________________________|____________| Parathyroid Gland | M + + + + + + + + + M + M + + + M + M M | 48 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + | 64 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + M + + + + + + + + + | 69 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + | 70 | Atrophy | | 1 2.0| __________________________________________________________________________|____________| Penis | + | 1 | __________________________________________________________________________|____________| Preputial Gland | + + + + + + + + + + + + + + + + | 55 | Abscess | | 1 3.0| Atrophy | | 1 2.0| Cyst | X X X X X X X X X X X | 42 | Dilatation | 3 4 2 4 4 4 4 3 | 25 3.1| Inflammation, Acute | 4 | 7 3.7| Inflammation, Chronic | 4 | 9 2.9| __________________________________________________________________________|____________| Prostate | + + + + + + + + + + A + + + + M + + + + | 67 | Atrophy | | 2 2.5| Inflammation, Chronic, Focal | 1 | 2 1.0| Inflammation, Chronic | | 2 1.0| __________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + | 70 | Depletion Cellular | | 3 2.7| Dilatation | 4 4 3 | 7 3.7| Fibrosis | | 1 4.0| __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + | 70 | Hypospermia | | 1 4.0| Seminiferous Tubule, Atrophy | | 1 3.0| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + | 70 | Hyperplasia, Neutrophil | 2 | 1 2.0| Myelofibrosis | | 1 2.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 73 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 4| 7| 2| 7| 7| 7| 7| 7| 7| 7| 6| 2| 7| 6| 7| 2| 7| 7| 6| 7| | | DAY ON TEST | 5| 4| 7| 4| 4| 4| 4| 4| 4| 4| 0| 7| 4| 2| 4| 7| 1| 4| 1| 4| | | | 4| 0| 5| 0| 0| 0| 0| 0| 0| 0| 1| 4| 0| 6| 0| 4| 0| 0| 0| 0| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 250 PPM | 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Lymph Node | | 1 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + M + | 67 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + M + + + + + M + M + | 65 | Congestion | | 3 3.0| Hyperplasia | 4 | 1 4.0| __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + | 69 | Congestion | | 1 2.0| Depletion Lymphoid | 3 | 4 2.0| Hematopoietic Cell Proliferation | 3 4 4 3 | 9 3.4| __________________________________________________________________________|____________| Thymus | + M + + M + + + M + + + + + + + + M M + | 58 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M | | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + | 69 | Ulcer | 4 | 1 4.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + | 70 | Mineralization, Focal | | 4 1.3| Mineralization | 2 2 2 2 2 2 2 2 3 1 2 2 | 34 2.1| _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + | 70 | Congestion | | 1 2.0| Hemorrhage, Focal | 3 | 1 3.0| Alveolar Epithelium, Hyperplasia | 2 2 | 4 1.8| Peribronchial, Inflammation, Chronic | 2 1 | 7 1.4| __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + | 70 | Degeneration, Hyaline | 1 | 3 1.7| Inflammation, Chronic, Focal | 2 2 2 | 10 2.0| Inflammation, Chronic | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 | 52 2.0| Metaplasia, Squamous | 2 | 1 2.0| Olfactory Epithelium, Hyperplasia | | 1 2.0| __________________________________________________________________________|____________| Trachea | + + + + + + + + + + M + + + + + + + + + | 68 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | None | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 74 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 4| 7| 2| 7| 7| 7| 7| 7| 7| 7| 6| 2| 7| 6| 7| 2| 7| 7| 6| 7| | | DAY ON TEST | 5| 4| 7| 4| 4| 4| 4| 4| 4| 4| 0| 7| 4| 2| 4| 7| 1| 4| 1| 4| | | | 4| 0| 5| 0| 0| 0| 0| 0| 0| 0| 1| 4| 0| 6| 0| 4| 0| 0| 0| 0| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 250 PPM | 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | __________________________________________________________________________________________________________________________________ URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + | 70 | Cyst | | 1 | Glomerulosclerosis | 2 | 1 2.0| Hyperplasia, Lymphoid | | 1 3.0| Infarct | | 1 2.0| Metaplasia, Osseous | | 1 3.0| Mineralization | 1 1 | 13 1.1| Nephropathy | 4 | 2 3.0| Renal Tubule, Regeneration | 1 1 2 1 2 1 2 1 1 2 2 1 1 | 40 1.5| __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + A + + + + + A + + + | 68 | Concretion | | 1 2.0| Ectasia | 4 | 1 4.0| Inflammation, Chronic, Focal | | 2 1.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 75 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 5| 7| 2| 7| 7| 7| 6| 7| 4| 6| 2| 4| 7| 7| 7| 7| 7| 7| 7| 2| 7| 4| 7| 4| 7| DAY ON TEST | 7| 3| 7| 3| 3| 3| 8| 3| 5| 5| 7| 5| 3| 3| 3| 3| 3| 3| 3| 7| 3| 5| 3| 5| 3| | 4| 0| 5| 0| 3| 0| 7| 6| 5| 4| 4| 4| 6| 6| 3| 3| 3| 3| 3| 5| 3| 4| 3| 5| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 500 PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | A + + + + + A + + A M + + + + + + + + + M + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + A + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + M + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + A + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | 3 3 2 2 3 2 3 2 2| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | A + + + + + + + + A + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | | Inflammation, Acute | 3 | __________________________________________________________________________| Intestine Small, Ileum | A + + + + + + + + A + M + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | X | Clear Cell Focus | X X X | Eosinophilic Focus | X X X | Fatty Change, Focal | 2 | Fatty Change | 1 2 2 3 3 3 2 1 1 2 | Inflammation, Chronic, Focal | | Necrosis, Focal | 3 | Thrombosis | X | Artery, Inflammation, Acute | 4 | __________________________________________________________________________| Mesentery | | Mineralization | | Fat, Necrosis | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2 2 1 | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Ulcer | 3 | __________________________________________________________________________| Stomach, Glandular | A + + + + + + + + A + + + + + + + + + + + + + + +| Erosion | | Hyperplasia | | Inflammation, Chronic | | __________________________________________________________________________| Tongue | + | Hemorrhage, Focal | | Pigmentation, Focal | 2 | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 76 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 5| 7| 2| 7| 7| 7| 6| 7| 4| 6| 2| 4| 7| 7| 7| 7| 7| 7| 7| 2| 7| 4| 7| 4| 7| DAY ON TEST | 7| 3| 7| 3| 3| 3| 8| 3| 5| 5| 7| 5| 3| 3| 3| 3| 3| 3| 3| 7| 3| 5| 3| 5| 3| | 4| 0| 5| 0| 3| 0| 7| 6| 5| 4| 4| 4| 6| 6| 3| 3| 3| 3| 3| 5| 3| 4| 3| 5| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 500 PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Fibrosis | 2 | Hyperplasia, Focal | 2 1 | Capsule, Hyperplasia | | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | | Hyperplasia | 2 2 1 3 4 1 | __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + M M + M + + + + + + + M + M +| __________________________________________________________________________| Pituitary Gland | I + + + + + + M + + + + + + + M + + + + + + + + +| __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + +| Cyst | X X X X X X X X X X X X X X| Depletion Cellular | | Dilatation | 4 4 4 3 4 4 3 4 4| Infiltration Cellular, Plasma Cell | | Inflammation, Acute | 4 4 | Inflammation, Chronic | 2 4 | __________________________________________________________________________| Prostate | + + M + + + + + + + + + + + + + + + + + + + + + +| Atrophy | | Inflammation, Acute | 3 | Inflammation, Chronic | 1 1 | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Cellular | | Dilatation | 3 3 | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Hypospermia | | Interstitial Cell, Hyperplasia | 2 | Seminiferous Tubule, Atrophy | 2 | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | + + + + + + + | __________________________________________________________________________| Bone Marrow | + + + + + + + + + A + + + + + + + + + + + + + + +| Hyperplasia, Neutrophil | 3 | Myelofibrosis | | __________________________________________________________________________| Lymph Node | + + | Renal, Lymphatic, Angiectasis | 2 | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + M + + +| Infiltration Cellular, Plasma Cell | | _____________________________________________________________________________________________________________________| Page 77 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 5| 7| 2| 7| 7| 7| 6| 7| 4| 6| 2| 4| 7| 7| 7| 7| 7| 7| 7| 2| 7| 4| 7| 4| 7| DAY ON TEST | 7| 3| 7| 3| 3| 3| 8| 3| 5| 5| 7| 5| 3| 3| 3| 3| 3| 3| 3| 7| 3| 5| 3| 5| 3| | 4| 0| 5| 0| 3| 0| 7| 6| 5| 4| 4| 4| 6| 6| 3| 3| 3| 3| 3| 5| 3| 4| 3| 5| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 500 PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + M + + + + + + + + + + + + + +| Congestion | 2 | Fibrosis | 2 | Infiltration Cellular, Plasma Cell | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Lymphoid | | Hematopoietic Cell Proliferation | 4 3 2 | Hyperplasia, Lymphoid | | Infiltration Cellular, Plasma Cell | | __________________________________________________________________________| Thymus | + I + + + + M + + + + M + M I + M + M + M M + + +| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperostosis | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Compression | | Hemorrhage, Focal | | Mineralization, Focal | 2 | Mineralization | 2 2 1 1 1 2 1 2 3 1 2 1 2 1 2 | __________________________________________________________________________| Peripheral Nerve | | __________________________________________________________________________| Spinal Cord | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic | | Alveolar Epithelium, Hyperplasia | 1 2 | Peribronchial, Inflammation, Chronic | 1 | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Degeneration, Hyaline | | Inflammation, Chronic, Focal | 2 2 2 | Inflammation, Chronic | 2 2 2 2 2 2 2 2 2 2 3 2 2 2 3 2 2 2 2| Metaplasia, Squamous | 2 | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Harderian Gland | + | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | _____________________________________________________________________________________________________________________| Page 78 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 5| 7| 2| 7| 7| 7| 6| 7| 4| 6| 2| 4| 7| 7| 7| 7| 7| 7| 7| 2| 7| 4| 7| 4| 7| DAY ON TEST | 7| 3| 7| 3| 3| 3| 8| 3| 5| 5| 7| 5| 3| 3| 3| 3| 3| 3| 3| 7| 3| 5| 3| 5| 3| | 4| 0| 5| 0| 3| 0| 7| 6| 5| 4| 4| 4| 6| 6| 3| 3| 3| 3| 3| 5| 3| 4| 3| 5| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 500 PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| URINARY SYSTEM - cont | | | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Casts | | Cyst | X | Glomerulosclerosis | 2 | Infarct | | Inflammation, Chronic, Focal | | Mineralization | 1 1 1 1 1 | Nephropathy | | Renal Tubule, Degeneration, Granular | 3 | Renal Tubule, Regeneration | 2 2 1 1 1 1 3 2 1 1 2 1 2| __________________________________________________________________________| Urinary Bladder | A + + + + + + + + + + + + + + + + + + + + + + + +| Calculus Micro Observation Only | | Concretion | | Perivascular, Inflammation, Chronic | | _____________________________________________________________________________________________________________________| Page 79 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 2| 2| 7| 7| 7| 6| 7| 7| 4| 7| 2| 7| 2| 7| 2| 4| 7| 4| 4| 7| 7| 2| 7| 7| 7| DAY ON TEST | 7| 7| 3| 3| 3| 1| 3| 3| 5| 3| 7| 3| 7| 3| 7| 5| 3| 5| 5| 3| 3| 7| 3| 3| 3| | 5| 5| 3| 6| 6| 8| 5| 5| 4| 6| 5| 6| 4| 5| 5| 4| 5| 5| 5| 3| 3| 5| 3| 3| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 500 PPM | 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + M + + + A + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + A + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + A + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | 3 3 3 2 4 | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + A + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + A + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | | Inflammation, Acute | | __________________________________________________________________________| Intestine Small, Ileum | + + + + + A + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | | Clear Cell Focus | X X | Eosinophilic Focus | X X X X X| Fatty Change, Focal | 2 | Fatty Change | 1 2 2 2 1 | Inflammation, Chronic, Focal | | Necrosis, Focal | | Thrombosis | | Artery, Inflammation, Acute | | __________________________________________________________________________| Mesentery | + | Mineralization | | Fat, Necrosis | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2 | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 1 2 2 1 2 | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Ulcer | | __________________________________________________________________________| Stomach, Glandular | + + + + + A + + + + + + + + + + + + + + + + + + +| Erosion | | Hyperplasia | | Inflammation, Chronic | 2 2 1 | __________________________________________________________________________| Tongue | + | Hemorrhage, Focal | 3 | Pigmentation, Focal | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 80 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 2| 2| 7| 7| 7| 6| 7| 7| 4| 7| 2| 7| 2| 7| 2| 4| 7| 4| 4| 7| 7| 2| 7| 7| 7| DAY ON TEST | 7| 7| 3| 3| 3| 1| 3| 3| 5| 3| 7| 3| 7| 3| 7| 5| 3| 5| 5| 3| 3| 7| 3| 3| 3| | 5| 5| 3| 6| 6| 8| 5| 5| 4| 6| 5| 6| 4| 5| 5| 4| 5| 5| 5| 3| 3| 5| 3| 3| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 500 PPM | 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Fibrosis | | Hyperplasia, Focal | 2 2 2 2 1 | Capsule, Hyperplasia | 2 | __________________________________________________________________________| Adrenal Medulla | I + + + + + + + + + + + + + + + + + + + + I + + M| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | | Hyperplasia | 1 4 3 1 2 1 1 | __________________________________________________________________________| Parathyroid Gland | + + + + M M + M + M + + + M + M M M M + M + + + M| __________________________________________________________________________| Pituitary Gland | + + + + + M + M + + + + + + + + M + + + + + + + +| __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + +| Cyst | X X X X X X X | Depletion Cellular | 2| Dilatation | 4 4 2 4 3 4 3 4 3 3 2 4 | Infiltration Cellular, Plasma Cell | | Inflammation, Acute | 4 4 | Inflammation, Chronic | 3 | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + M M + + M + + + + +| Atrophy | | Inflammation, Acute | | Inflammation, Chronic | | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Cellular | | Dilatation | | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Hypospermia | | Interstitial Cell, Hyperplasia | | Seminiferous Tubule, Atrophy | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Neutrophil | | Myelofibrosis | 2 | __________________________________________________________________________| Lymph Node | | Renal, Lymphatic, Angiectasis | | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + M + + + M + + +| Infiltration Cellular, Plasma Cell | | _____________________________________________________________________________________________________________________| Page 81 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 2| 2| 7| 7| 7| 6| 7| 7| 4| 7| 2| 7| 2| 7| 2| 4| 7| 4| 4| 7| 7| 2| 7| 7| 7| DAY ON TEST | 7| 7| 3| 3| 3| 1| 3| 3| 5| 3| 7| 3| 7| 3| 7| 5| 3| 5| 5| 3| 3| 7| 3| 3| 3| | 5| 5| 3| 6| 6| 8| 5| 5| 4| 6| 5| 6| 4| 5| 5| 4| 5| 5| 5| 3| 3| 5| 3| 3| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 500 PPM | 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + M + + + + + + + M + + + + + + + +| Congestion | | Fibrosis | | Infiltration Cellular, Plasma Cell | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Lymphoid | | Hematopoietic Cell Proliferation | 2 3 3 | Hyperplasia, Lymphoid | | Infiltration Cellular, Plasma Cell | | __________________________________________________________________________| Thymus | + + + M + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic | 4 | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperostosis | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Compression | | Hemorrhage, Focal | | Mineralization, Focal | 1 1 1 1 | Mineralization | 2 2 2 2 2 2 2 | __________________________________________________________________________| Peripheral Nerve | | __________________________________________________________________________| Spinal Cord | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic | 2 | Alveolar Epithelium, Hyperplasia | 2 | Peribronchial, Inflammation, Chronic | 2 1 | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Degeneration, Hyaline | 2 2 | Inflammation, Chronic, Focal | 2 2 2 2 2 2 | Inflammation, Chronic | 2 2 2 2 2 3 2 2 2 2 2 2 2 2 2 2 2| Metaplasia, Squamous | | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Harderian Gland | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | _____________________________________________________________________________________________________________________| Page 82 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 _____________________________________________________________________________________________________________________ | 2| 2| 7| 7| 7| 6| 7| 7| 4| 7| 2| 7| 2| 7| 2| 4| 7| 4| 4| 7| 7| 2| 7| 7| 7| DAY ON TEST | 7| 7| 3| 3| 3| 1| 3| 3| 5| 3| 7| 3| 7| 3| 7| 5| 3| 5| 5| 3| 3| 7| 3| 3| 3| | 5| 5| 3| 6| 6| 8| 5| 5| 4| 6| 5| 6| 4| 5| 5| 4| 5| 5| 5| 3| 3| 5| 3| 3| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 500 PPM | 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| _____________________________________________________________________________________________________________________| URINARY SYSTEM - cont | | | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Casts | | Cyst | | Glomerulosclerosis | | Infarct | | Inflammation, Chronic, Focal | | Mineralization | 1 1 1 1 1 1 | Nephropathy | 2| Renal Tubule, Degeneration, Granular | | Renal Tubule, Regeneration | 1 2 1 1 2 2 1 1 1 2 2| __________________________________________________________________________| Urinary Bladder | + + + + + A + + + + + + + + + + + + + + + + + + +| Calculus Micro Observation Only | X | Concretion | | Perivascular, Inflammation, Chronic | | _____________________________________________________________________________________________________________________| Page 83 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 6| 7| 2| 7| 4| 7| 4| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| | | DAY ON TEST | 8| 3| 7| 3| 5| 3| 5| 3| 3| 4| 3| 3| 0| 3| 2| 3| 3| 3| 0| 3| | | | 4| 6| 5| 3| 5| 3| 4| 3| 4| 7| 4| 4| 0| 3| 6| 4| 4| 3| 7| 3| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 500 PPM | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + + + A + | 62 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + A + | 67 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + M + + + + + A + | 67 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + A + | 67 | Hyperplasia, Lymphoid | 2 2 3 3 3 3 3 3 3 3 | 24 2.7| __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + A + | 68 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + A + | 66 | Hyperplasia, Lymphoid | 4 | 1 4.0| Inflammation, Acute | | 1 3.0| __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + A + | 65 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + | 70 | Basophilic Focus | | 1 | Clear Cell Focus | X | 6 | Eosinophilic Focus | X X X X X X | 14 | Fatty Change, Focal | | 2 2.0| Fatty Change | 1 2 2 2 1 2 2 2 2 | 24 1.8| Inflammation, Chronic, Focal | 2 | 1 2.0| Necrosis, Focal | 1 2 1 1 4 | 6 2.0| Thrombosis | | 1 | Artery, Inflammation, Acute | | 1 4.0| __________________________________________________________________________|____________| Mesentery | + | 2 | Mineralization | 4 | 1 4.0| Fat, Necrosis | 4 | 1 4.0| __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + | 70 | Inflammation, Chronic, Focal | | 1 2.0| __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + | 70 | Inflammation, Chronic, Focal | | 8 1.6| __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + | 70 | Ulcer | 3 | 2 3.0| __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + | 67 | Erosion | 2 2 | 2 2.0| Hyperplasia | 3 | 1 3.0| Inflammation, Chronic | | 3 1.7| __________________________________________________________________________|____________| Tongue | | 2 | Hemorrhage, Focal | | 1 3.0| Pigmentation, Focal | | 1 2.0| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 84 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 6| 7| 2| 7| 4| 7| 4| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| | | DAY ON TEST | 8| 3| 7| 3| 5| 3| 5| 3| 3| 4| 3| 3| 0| 3| 2| 3| 3| 3| 0| 3| | | | 4| 6| 5| 3| 5| 3| 4| 3| 4| 7| 4| 4| 0| 3| 6| 4| 4| 3| 7| 3| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 500 PPM | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | __________________________________________________________________________________________________________________________________ CARDIOVASCULAR SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + | 70 | Inflammation, Chronic, Focal | 2 | 1 2.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + | 70 | Fibrosis | | 1 2.0| Hyperplasia, Focal | 2 2 2 2 2 1 | 13 1.8| Capsule, Hyperplasia | | 1 2.0| __________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + M + | 66 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + | 70 | Atrophy | 3 | 1 3.0| Hyperplasia | 3 1 2 2 4 2 2 | 20 2.1| __________________________________________________________________________|____________| Parathyroid Gland | + + + + M + + + M + + M M + + + M + + + | 49 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + | 64 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Preputial Gland | + + + + + + + + + + + + + + + + + M + | 54 | Cyst | X X X X X X X X X X X X X X | 35 | Depletion Cellular | | 1 2.0| Dilatation | 4 3 4 4 4 3 4 | 28 3.6| Infiltration Cellular, Plasma Cell | 3 | 1 3.0| Inflammation, Acute | | 4 4.0| Inflammation, Chronic | 3 4 4 | 6 3.3| __________________________________________________________________________|____________| Prostate | + + + + + + M + + + + + + + + + + + + + | 65 | Atrophy | 3 | 1 3.0| Inflammation, Acute | | 1 3.0| Inflammation, Chronic | | 2 1.0| __________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + | 70 | Depletion Cellular | 2 4 | 2 3.0| Dilatation | 3 | 3 3.0| __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + | 70 | Hypospermia | 2 2 | 2 2.0| Interstitial Cell, Hyperplasia | | 1 2.0| Seminiferous Tubule, Atrophy | 2 | 2 2.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 85 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 6| 7| 2| 7| 4| 7| 4| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| | | DAY ON TEST | 8| 3| 7| 3| 5| 3| 5| 3| 3| 4| 3| 3| 0| 3| 2| 3| 3| 3| 0| 3| | | | 4| 6| 5| 3| 5| 3| 4| 3| 4| 7| 4| 4| 0| 3| 6| 4| 4| 3| 7| 3| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 500 PPM | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | __________________________________________________________________________________________________________________________________ GENITAL SYSTEM - cont | | | | | | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Blood | | 7 | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + | 69 | Hyperplasia, Neutrophil | 4 3 | 3 3.3| Myelofibrosis | | 1 2.0| __________________________________________________________________________|____________| Lymph Node | | 2 | Renal, Lymphatic, Angiectasis | | 1 2.0| __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + A + | 66 | Infiltration Cellular, Plasma Cell | 4 | 1 4.0| __________________________________________________________________________|____________| Lymph Node, Mesenteric | M + + + + + + + + + + + + + + + + + A + | 65 | Congestion | 2 | 2 2.0| Fibrosis | | 1 2.0| Infiltration Cellular, Plasma Cell | 4 | 1 4.0| __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + | 70 | Depletion Lymphoid | 2 | 1 2.0| Hematopoietic Cell Proliferation | 4 4 | 8 3.1| Hyperplasia, Lymphoid | 3 | 1 3.0| Infiltration Cellular, Plasma Cell | 3 | 1 3.0| __________________________________________________________________________|____________| Thymus | + + + + + + + + + M + + M + M + + + M + | 56 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M + M M M + M M M M M M | 2 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + | 70 | Inflammation, Chronic | | 1 4.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + | 70 | Hyperostosis | 3 | 1 3.0| _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + | 70 | Compression | 4 | 1 4.0| Hemorrhage, Focal | 3 | 1 3.0| Mineralization, Focal | | 5 1.2| Mineralization | 1 3 2 2 2 3 2 1 2 1 2 3 | 34 1.8| __________________________________________________________________________|____________| Peripheral Nerve | + | 1 | __________________________________________________________________________|____________| Spinal Cord | + | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 86 NTP Experiment-Test: 05150-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLPHENIDATE HYDROCHLORIDE Date: 04/23/97 Route: DOSED FEED Time: 11:53:50 __________________________________________________________________________________________________________________________________ | 6| 7| 2| 7| 4| 7| 4| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| | | DAY ON TEST | 8| 3| 7| 3| 5| 3| 5| 3| 3| 4| 3| 3| 0| 3| 2| 3| 3| 3| 0| 3| | | | 4| 6| 5| 3| 5| 3| 4| 3| 4| 7| 4| 4| 0| 3| 6| 4| 4| 3| 7| 3| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 500 PPM | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | __________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - cont | | | | | | | | | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + | 70 | Inflammation, Chronic | | 1 2.0| Alveolar Epithelium, Hyperplasia | | 3 1.7| Peribronchial, Inflammation, Chronic | | 3 1.3| __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + | 70 | Degeneration, Hyaline | | 2 2.0| Inflammation, Chronic, Focal | 2 | 10 2.0| Inflammation, Chronic | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 | 52 2.1| Metaplasia, Squamous | | 1 2.0| __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Harderian Gland | + | 2 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + | 70 | Casts | X | 1 | Cyst | X X | 3 | Glomerulosclerosis | | 1 2.0| Infarct | 2 | 1 2.0| Inflammation, Chronic, Focal | 2 | 1 2.0| Mineralization | 2 2 1 2 2 1 2 | 18 1.3| Nephropathy | | 1 2.0| Renal Tubule, Degeneration, Granular | 4 | 2 3.5| Renal Tubule, Regeneration | 1 1 1 2 2 1 1 2 2 2 1 2 | 36 1.5| __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + A + | 67 | Calculus Micro Observation Only | | 1 | Concretion | 3 | 1 3.0| Perivascular, Inflammation, Chronic | 2 | 1 2.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 87 ------------------------------------------------------------ ---------- END OF REPORT ---------- ------------------------------------------------------------