TDMS Study 05200-06 Pathology Tables
NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 Facility: Battelle Northwest Chemical CAS #: 75-52-5 Lock Date: 06/22/92 Cage Range: All Reasons For Removal: All Removal Date Range: All Treatment Groups: Include All Note: Animals arranged according to CID number Page 1 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 5| 7| 7| 6| 6| 7| 3| 7| 7| 6| 3| 7| 5| 6| 7| 4| 7| 7| 7| 7| 7| 6| 7| 7| 7| DAY ON TEST | 7| 3| 3| 6| 6| 3| 7| 3| 3| 3| 0| 0| 9| 9| 3| 0| 3| 3| 3| 3| 3| 0| 3| 3| 3| | 4| 7| 4| 3| 7| 7| 6| 4| 7| 2| 1| 7| 7| 3| 5| 1| 5| 5| 4| 5| 5| 9| 6| 4| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| CONTROL | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + M + + + + + + + + + +| __________________________________________________________________________| Gallbladder | A + + + + + + M + + A A + M I + + + + + + + + + +| Lymphoma Malignant | | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + M + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | X | __________________________________________________________________________| Intestine Small, Duodenum | A + + + + + + + + + + A + + + + + + + + + + + + +| Lymphoma Malignant | X | __________________________________________________________________________| Intestine Small, Jejunum | A + + + + + + + + + + A + + + + + + + + + + + + +| Lymphoma Malignant | X | Peyer's Patch, Lymphoma Malignant | | __________________________________________________________________________| Intestine Small, Ileum | A + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | | Hepatocellular Carcinoma | X X | Hepatocellular Carcinoma, Multiple | X | Hepatocellular Adenoma | X X X X X X X X | Hepatocellular Adenoma, Multiple | X | Hepatocholangiocarcinoma | | Histiocytic Sarcoma | | Lymphoma Malignant | X X X | __________________________________________________________________________| Mesentery | + + + + | Sarcoma, Metastatic, Skin | | __________________________________________________________________________| Oral Mucosa | | Hepatocholangiocarcinoma, Metastatic,| | Liver | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | Squamous Cell Papilloma | X | __________________________________________________________________________| Stomach, Glandular | A + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Hepatocholangiocarcinoma, Metastatic,| | Liver | | Histiocytic Sarcoma | | Lymphoma Malignant | | _____________________________________________________________________________________________________________________| Page 2 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 5| 7| 7| 6| 6| 7| 3| 7| 7| 6| 3| 7| 5| 6| 7| 4| 7| 7| 7| 7| 7| 6| 7| 7| 7| DAY ON TEST | 7| 3| 3| 6| 6| 3| 7| 3| 3| 3| 0| 0| 9| 9| 3| 0| 3| 3| 3| 3| 3| 0| 3| 3| 3| | 4| 7| 4| 3| 7| 7| 6| 4| 7| 2| 1| 7| 7| 3| 5| 1| 5| 5| 4| 5| 5| 9| 6| 4| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| CONTROL | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | X | Capsule, Adenoma | X | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | + + M + + M + M M + + + + + + + M I M + M + M + +| Carcinoma | | __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | Pars Distalis, Adenoma | X X X X X X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | Follicular Cell, Adenoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | M + + + + + + + + + + + + + + M + + M + + + M + +| __________________________________________________________________________| Ovary | + + + I + + + + + + + + + + + + + + + + + + + I +| Cystadenoma | | Histiocytic Sarcoma | | Lymphoma Malignant | X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X| Hemangioma | X | Histiocytic Sarcoma | | Lymphoma Malignant | | Polyp Stromal | X | __________________________________________________________________________| Vagina | + | Histiocytic Sarcoma | X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X | Histiocytic Sarcoma | | Lymphoma Malignant | X X | Mast Cell Tumor Malignant | X | __________________________________________________________________________| Lymph Node | + + + + | Lymphoma Malignant | X | Iliac, Lymphoma Malignant | X | Renal, Lymphoma Malignant | X X X | _____________________________________________________________________________________________________________________| Page 3 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 5| 7| 7| 6| 6| 7| 3| 7| 7| 6| 3| 7| 5| 6| 7| 4| 7| 7| 7| 7| 7| 6| 7| 7| 7| DAY ON TEST | 7| 3| 3| 6| 6| 3| 7| 3| 3| 3| 0| 0| 9| 9| 3| 0| 3| 3| 3| 3| 3| 0| 3| 3| 3| | 4| 7| 4| 3| 7| 7| 6| 4| 7| 2| 1| 7| 7| 3| 5| 1| 5| 5| 4| 5| 5| 9| 6| 4| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| CONTROL | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | __________________________________________________________________________| Lymph Node, Bronchial | M M + + + + M + + + + + M + + + + + + + M + + + +| Hepatocholangiocarcinoma, Metastatic,| | Liver | | Lymphoma Malignant | X X | __________________________________________________________________________| Lymph Node, Mandibular | + M + + + + + + + M + + + M M M + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant | X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + M + +| Histiocytic Sarcoma | | Lymphoma Malignant | X X X | __________________________________________________________________________| Lymph Node, Mediastinal | M M + + + + M + + + M + + M + + + + + + + + + M +| Hepatocholangiocarcinoma, Metastatic,| | Liver | | Lymphoma Malignant | X X X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | X X X X | Mast Cell Tumor Malignant | X | __________________________________________________________________________| Thymus | + + + + + + + + + + + M + + + + + + + + M + M + +| Hepatocholangiocarcinoma, Metastatic,| | Liver | | Lymphoma Malignant | X X X | Mast Cell Tumor Malignant | X | Thymoma Benign | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma | X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Sarcoma | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X X| Hepatocellular Carcinoma, Metastatic,| | Liver | | Hepatocholangiocarcinoma, Metastatic,| | _____________________________________________________________________________________________________________________| Page 4 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 5| 7| 7| 6| 6| 7| 3| 7| 7| 6| 3| 7| 5| 6| 7| 4| 7| 7| 7| 7| 7| 6| 7| 7| 7| DAY ON TEST | 7| 3| 3| 6| 6| 3| 7| 3| 3| 3| 0| 0| 9| 9| 3| 0| 3| 3| 3| 3| 3| 0| 3| 3| 3| | 4| 7| 4| 3| 7| 7| 6| 4| 7| 2| 1| 7| 7| 3| 5| 1| 5| 5| 4| 5| 5| 9| 6| 4| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| CONTROL | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Liver | | Histiocytic Sarcoma | | Lymphoma Malignant | X X | Mediastinum, Hemangioma | | Mediastinum, Hemangiosarcoma | X | Mediastinum, | | Hepatocholangiocarcinoma, | | Metastatic, Liver | | Mediastinum, Mast Cell Tumor | | Malignant | X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X | Adenoma, Multiple | | Carcinoma | X | Lymphoma Malignant | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Hepatocholangiocarcinoma, Metastatic,| | Liver | | Lymphoma Malignant | X X | __________________________________________________________________________| Urinary Bladder | + + + + + + + + I + + + + + + + + + + + + + + + +| Lymphoma Malignant | | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant | X X X X X | _____________________________________________________________________________________________________________________| Page 5 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 7| 6| 7| 5| 3| 6| 6| 7| 7| 6| 4| 4| 7| 7| 6| 7| 7| 7| 7| 6| 7| 6| 7| 6| | DAY ON TEST | 3| 3| 5| 3| 7| 8| 1| 6| 3| 1| 1| 3| 3| 3| 3| 9| 0| 3| 3| 3| 8| 3| 9| 3| 3| | | 6| 6| 7| 5| 6| 5| 7| 7| 7| 6| 8| 6| 6| 4| 5| 3| 7| 6| 4| 4| 1| 5| 3| 6| 2| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | CONTROL | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + M + + + + + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Gallbladder | + + + + + + A + + + + I + + + + M + + M + + + + +| 40 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + A + + + + + + + + + + + + + + + + + +| 49 | Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + +| 47 | Lymphoma Malignant | | 1 | Peyer's Patch, Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + A + + + + + + + + + + + + M + + + + +| 47 | Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemangiosarcoma | X X | 2 | Hepatocellular Carcinoma | X X X X X | 7 | Hepatocellular Carcinoma, Multiple | X X | 3 | Hepatocellular Adenoma | X X X | 11 | Hepatocellular Adenoma, Multiple | X X | 3 | Hepatocholangiocarcinoma | X | 1 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant | X X X X | 7 | __________________________________________________________________________|____________| Mesentery | + + +| 7 | Sarcoma, Metastatic, Skin | X | 1 | __________________________________________________________________________|____________| Oral Mucosa | + | 1 | Hepatocholangiocarcinoma, Metastatic,| | | Liver | X | 1 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant | X X | 2 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + M + +| 49 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant | X | 1 | Squamous Cell Papilloma | | 1 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Lymphoma Malignant | X | 1 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 6 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 7| 6| 7| 5| 3| 6| 6| 7| 7| 6| 4| 4| 7| 7| 6| 7| 7| 7| 7| 6| 7| 6| 7| 6| | DAY ON TEST | 3| 3| 5| 3| 7| 8| 1| 6| 3| 1| 1| 3| 3| 3| 3| 9| 0| 3| 3| 3| 8| 3| 9| 3| 3| | | 6| 6| 7| 5| 6| 5| 7| 7| 7| 6| 8| 6| 6| 4| 5| 3| 7| 6| 4| 4| 1| 5| 3| 6| 2| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | CONTROL | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ CARDIOVASCULAR SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hepatocholangiocarcinoma, Metastatic,| | | Liver | X | 1 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant | X X | 2 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant | | 1 | Capsule, Adenoma | | 1 | __________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Parathyroid Gland | + + + M + M M + + I + + M M + + + + + + + M + M +| 33 | Carcinoma | X | 1 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant | X | 1 | Pars Distalis, Adenoma | X X X | 9 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant | X | 1 | Follicular Cell, Adenoma | X X X | 4 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Clitoral Gland | + M + + M + + + + + + M M M + + + + + + + M + M +| 39 | __________________________________________________________________________|____________| Ovary | + + I + + + + + + + + + + + + + + + + + + + + + +| 47 | Cystadenoma | X X| 2 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant | X X X | 4 | __________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Adenoma | | 1 | Hemangioma | X | 2 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant | X | 1 | Polyp Stromal | X X X | 4 | __________________________________________________________________________|____________| Vagina | | 1 | Histiocytic Sarcoma | | 1 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 7 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 7| 6| 7| 5| 3| 6| 6| 7| 7| 6| 4| 4| 7| 7| 6| 7| 7| 7| 7| 6| 7| 6| 7| 6| | DAY ON TEST | 3| 3| 5| 3| 7| 8| 1| 6| 3| 1| 1| 3| 3| 3| 3| 9| 0| 3| 3| 3| 8| 3| 9| 3| 3| | | 6| 6| 7| 5| 6| 5| 7| 7| 7| 6| 8| 6| 6| 4| 5| 3| 7| 6| 4| 4| 1| 5| 3| 6| 2| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | CONTROL | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemangiosarcoma | | 1 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant | X X | 4 | Mast Cell Tumor Malignant | | 1 | __________________________________________________________________________|____________| Lymph Node | + + + + | 8 | Lymphoma Malignant | X X | 3 | Iliac, Lymphoma Malignant | X X | 3 | Renal, Lymphoma Malignant | X X | 5 | __________________________________________________________________________|____________| Lymph Node, Bronchial | + M + + + + + + M M + + M + + + + + + + M + M + +| 39 | Hepatocholangiocarcinoma, Metastatic,| | | Liver | X | 1 | Lymphoma Malignant | X X X X | 6 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + M + + + + + + + + + + + + + + + + + + + + M + +| 43 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant | X X X | 4 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + M + + + + + + + + + + + + + + + + + + + + +| 48 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant | X X X X | 7 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | + + M + + + + M M + + + M + + M + M + + + + + + +| 38 | Hepatocholangiocarcinoma, Metastatic,| | | Liver | X | 1 | Lymphoma Malignant | X X X X | 7 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant | X X X X | 8 | Mast Cell Tumor Malignant | | 1 | __________________________________________________________________________|____________| Thymus | + M + + + + + + + I + + + + + M + + + + M + + + +| 43 | Hepatocholangiocarcinoma, Metastatic,| | | Liver | X | 1 | Lymphoma Malignant | X X | 5 | Mast Cell Tumor Malignant | | 1 | Thymoma Benign | X| 1 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Carcinoma | X | 2 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + A + + + + + + + + + + + +| 49 | Subcutaneous Tissue, Sarcoma | X | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 8 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 7| 6| 7| 5| 3| 6| 6| 7| 7| 6| 4| 4| 7| 7| 6| 7| 7| 7| 7| 6| 7| 6| 7| 6| | DAY ON TEST | 3| 3| 5| 3| 7| 8| 1| 6| 3| 1| 1| 3| 3| 3| 3| 9| 0| 3| 3| 3| 8| 3| 9| 3| 3| | | 6| 6| 7| 5| 6| 5| 7| 7| 7| 6| 8| 6| 6| 4| 5| 3| 7| 6| 4| 4| 1| 5| 3| 6| 2| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | CONTROL | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ INTEGUMENTARY SYSTEM - cont | | | | | | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + + + + I + + + + + + + + + + + + +| 49 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Alveolar/Bronchiolar Adenoma | X | 3 | Hepatocellular Carcinoma, Metastatic,| | | Liver | X X | 2 | Hepatocholangiocarcinoma, Metastatic,| | | Liver | X | 1 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant | X X X | 5 | Mediastinum, Hemangioma | X | 1 | Mediastinum, Hemangiosarcoma | | 1 | Mediastinum, | | | Hepatocholangiocarcinoma, | | | Metastatic, Liver | X | 1 | Mediastinum, Mast Cell Tumor | | | Malignant | | 1 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + | 49 | Adenoma | X X | 4 | Adenoma, Multiple | X | 1 | Carcinoma | | 1 | Lymphoma Malignant | X | 1 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hepatocholangiocarcinoma, Metastatic,| | | Liver | X | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 9 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 7| 6| 7| 5| 3| 6| 6| 7| 7| 6| 4| 4| 7| 7| 6| 7| 7| 7| 7| 6| 7| 6| 7| 6| | DAY ON TEST | 3| 3| 5| 3| 7| 8| 1| 6| 3| 1| 1| 3| 3| 3| 3| 9| 0| 3| 3| 3| 8| 3| 9| 3| 3| | | 6| 6| 7| 5| 6| 5| 7| 7| 7| 6| 8| 6| 6| 4| 5| 3| 7| 6| 4| 4| 1| 5| 3| 6| 2| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | CONTROL | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ URINARY SYSTEM - cont | | | | | | Lymphoma Malignant | X X | 4 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Lymphoma Malignant | X | 1 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant | X X X X | 9 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 10 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 7| 7| 7| 6| 7| 7| 4| 5| 7| 6| 5| 5| 6| 3| 6| 5| 7| 7| 7| 7| 7| 7| 7| 5| 7| DAY ON TEST | 3| 3| 3| 3| 3| 3| 2| 5| 3| 6| 6| 6| 3| 4| 9| 3| 3| 3| 3| 3| 3| 3| 3| 8| 3| | 5| 6| 5| 7| 6| 6| 6| 5| 7| 3| 4| 9| 9| 5| 3| 4| 1| 6| 6| 4| 5| 4| 7| 1| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 188 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + A + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + A + + + A + + A + A + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + A + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + A + + + + + + A + A + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + A + + + A + + A + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + A + M + A + + M + A + + + + + + + + + + + +| Lymphoma Malignant | X | __________________________________________________________________________| Intestine Small, Jejunum | + + + A + + + A + + A + A + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + A + + + A + + + + A + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + A + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Carcinoma | X X X X X X X X | Hepatocellular Adenoma | X X X X| Hepatocellular Adenoma, Multiple | X X X X X X X X | Lymphoma Malignant | X X | __________________________________________________________________________| Mesentery | + + + + + + + + +| Hemangioma | | Hepatocellular Carcinoma, Metastatic,| | Liver | | Sarcoma | | __________________________________________________________________________| Pancreas | + + + A + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | __________________________________________________________________________| Salivary Glands | + + + A + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X | Lymphoma Malignant | | __________________________________________________________________________| Stomach, Forestomach | + + + A + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | Squamous Cell Papilloma | | __________________________________________________________________________| Stomach, Glandular | + + + A + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Lymphoma Malignant | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Blood Vessel | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Carcinoma, Metastatic,| | Liver | | Lymphoma Malignant | X | Pericardium, Hepatocellular | | Carcinoma, Metastatic, Liver | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + A + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | X | __________________________________________________________________________| Adrenal Medulla | + + + A + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 11 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 7| 7| 7| 6| 7| 7| 4| 5| 7| 6| 5| 5| 6| 3| 6| 5| 7| 7| 7| 7| 7| 7| 7| 5| 7| DAY ON TEST | 3| 3| 3| 3| 3| 3| 2| 5| 3| 6| 6| 6| 3| 4| 9| 3| 3| 3| 3| 3| 3| 3| 3| 8| 3| | 5| 6| 5| 7| 6| 6| 6| 5| 7| 3| 4| 9| 9| 5| 3| 4| 1| 6| 6| 4| 5| 4| 7| 1| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 188 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Islets, Pancreatic | + + + A + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | + + + M + + + M + + M M + M + M + M + + + + + + M| __________________________________________________________________________| Pituitary Gland | + + + A + + + + + + + + + + + + + + + + + + + + +| Pars Distalis, Adenoma | X X X X X | Pars Intermedia, Adenoma | X | __________________________________________________________________________| Thyroid Gland | + + + A + + + + + + + + + + + + + + + + + + + + +| Follicular Cell, Adenoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | + + + M + + + M + + + M + + M + + M M + M + + + M| __________________________________________________________________________| Ovary | + + + A + + + + + I + + + + + + + + + + + + + + +| Cystadenoma | X | Granulosa Cell Tumor Benign | X X | Lymphoma Malignant | | __________________________________________________________________________| Uterus | + + + A + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Deciduoma Benign | X | Hemangioma | X X | Lymphoma Malignant | | Polyp Stromal | X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + A + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | X | __________________________________________________________________________| Lymph Node | + + + + + | Lymphoma Malignant | X X X | Iliac, Lymphoma Malignant | X X | Renal, Lymphoma Malignant | X | __________________________________________________________________________| Lymph Node, Bronchial | M + + A M + + + + + + + + + + M + + M M + M + + M| Hepatocellular Carcinoma, Metastatic,| | Liver | X | Lymphoma Malignant | X X | __________________________________________________________________________| Lymph Node, Mandibular | + M M A M M + + + M + + + M + + + + M + + M + + +| Lymphoma Malignant | X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + M + + M + + + + + + M + + + + + + + + + + +| Lymphoma Malignant | X X X | __________________________________________________________________________| Lymph Node, Mediastinal | M M + A + + + + + + + M + M + + + + + + + + + M I| Hepatocellular Carcinoma, Metastatic,| | Liver | | Lymphoma Malignant | X X | __________________________________________________________________________| Spleen | + + + A + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 12 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 7| 7| 7| 6| 7| 7| 4| 5| 7| 6| 5| 5| 6| 3| 6| 5| 7| 7| 7| 7| 7| 7| 7| 5| 7| DAY ON TEST | 3| 3| 3| 3| 3| 3| 2| 5| 3| 6| 6| 6| 3| 4| 9| 3| 3| 3| 3| 3| 3| 3| 3| 8| 3| | 5| 6| 5| 7| 6| 6| 6| 5| 7| 3| 4| 9| 9| 5| 3| 4| 1| 6| 6| 4| 5| 4| 7| 1| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 188 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Hemangiosarcoma | X | Lymphoma Malignant | X X X X X | __________________________________________________________________________| Thymus | + + + A M + M + + + M + + + + + + + M M + I + + +| Hepatocellular Carcinoma, Metastatic,| | Liver | | Lymphoma Malignant | X X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma | X | __________________________________________________________________________| Skin | + + + + + + + M + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Sarcoma | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + A + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X X | Alveolar/Bronchiolar Adenoma, | | Multiple | X | Alveolar/Bronchiolar Carcinoma | X X X| Hepatocellular Carcinoma, Metastatic,| | Liver | X X X | Lymphoma Malignant | X | Mediastinum, Hepatocellular | | Carcinoma, Metastatic, Liver | X | __________________________________________________________________________| Nose | + + + A + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | __________________________________________________________________________| Trachea | + + + A + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X X | Adenoma, Multiple | X | Carcinoma | X| _____________________________________________________________________________________________________________________| URINARY SYSTEM | | _____________________________________________________________________________________________________________________| Page 13 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 7| 7| 7| 6| 7| 7| 4| 5| 7| 6| 5| 5| 6| 3| 6| 5| 7| 7| 7| 7| 7| 7| 7| 5| 7| DAY ON TEST | 3| 3| 3| 3| 3| 3| 2| 5| 3| 6| 6| 6| 3| 4| 9| 3| 3| 3| 3| 3| 3| 3| 3| 8| 3| | 5| 6| 5| 7| 6| 6| 6| 5| 7| 3| 4| 9| 9| 5| 3| 4| 1| 6| 6| 4| 5| 4| 7| 1| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 188 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| URINARY SYSTEM - cont | | | | | | __________________________________________________________________________| Kidney | + + + A + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | X X | __________________________________________________________________________| Urinary Bladder | + + + A + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | X X X X X | _____________________________________________________________________________________________________________________| Page 14 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 5| 3| 5| 7| 6| 7| 6| 7| 7| 4| 7| 7| 4| 7| 7| 7| 6| 7| 7| 7| 6| 7| | DAY ON TEST | 3| 6| 3| 7| 5| 6| 3| 9| 3| 5| 3| 3| 1| 3| 3| 4| 3| 3| 3| 4| 3| 3| 3| 5| 3| | | 6| 5| 7| 9| 7| 9| 5| 3| 5| 6| 7| 4| 6| 7| 5| 1| 6| 5| 6| 6| 5| 4| 7| 3| 5| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A | 188 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + A + + + + + + + + + + + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + M + + + + + + + + + + +| 45 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + A + + + + + + + + + + + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + A + + + + + + + + + + + + + + + + + + + + +| 46 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + A + + + + + + + + + + + + + + + + + + + + +| 46 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + A + M + + + + + + + + + + + + + + + + + + +| 43 | Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + A + + + + + + + + + + + + + + + + + + + + +| 45 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + A + + + + + + + + + + + + + + + + + + + + +| 46 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Hepatocellular Carcinoma | X X X X X X | 14 | Hepatocellular Adenoma | X X X X X X X X| 12 | Hepatocellular Adenoma, Multiple | X X X X X | 13 | Lymphoma Malignant | X | 3 | __________________________________________________________________________|____________| Mesentery | + + + + + | 14 | Hemangioma | X | 1 | Hepatocellular Carcinoma, Metastatic,| | | Liver | X | 1 | Sarcoma | X | 1 | __________________________________________________________________________|____________| Pancreas | + + + A + + + + + + + + + + + + + + + + + + + + +| 48 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Salivary Glands | + + + A + + + + + + + + + + + + + + + + + + + + +| 48 | Hemangiosarcoma | | 1 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + A + + + + + + + + + + + + + + + + + + + + +| 48 | Lymphoma Malignant | X | 1 | Squamous Cell Papilloma | X | 1 | __________________________________________________________________________|____________| Stomach, Glandular | + + + A + + + + + + + + + + + + + + + + + + + + +| 48 | Adenoma | | 1 | Lymphoma Malignant | X | 1 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Blood Vessel | + | 1 | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hepatocellular Carcinoma, Metastatic,| | | Liver | X | 1 | Lymphoma Malignant | X | 2 | Pericardium, Hepatocellular | | | Carcinoma, Metastatic, Liver | X | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 15 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 5| 3| 5| 7| 6| 7| 6| 7| 7| 4| 7| 7| 4| 7| 7| 7| 6| 7| 7| 7| 6| 7| | DAY ON TEST | 3| 6| 3| 7| 5| 6| 3| 9| 3| 5| 3| 3| 1| 3| 3| 4| 3| 3| 3| 4| 3| 3| 3| 5| 3| | | 6| 5| 7| 9| 7| 9| 5| 3| 5| 6| 7| 4| 6| 7| 5| 1| 6| 5| 6| 6| 5| 4| 7| 3| 5| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A | 188 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ CARDIOVASCULAR SYSTEM - cont | | | | | | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | + + + A + + + + + + + + + + + + + + + + + + + + +| 48 | Lymphoma Malignant | X | 2 | __________________________________________________________________________|____________| Adrenal Medulla | + + + A + + + + + + + + + + + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + A + + + + + + + + + + + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Parathyroid Gland | M + M M + + + M + + + + + M + + M + M + + + + + I| 34 | __________________________________________________________________________|____________| Pituitary Gland | + + + A + + + + + + + + M M + + + + + + + + + + +| 46 | Pars Distalis, Adenoma | X X X X X | 10 | Pars Intermedia, Adenoma | | 1 | __________________________________________________________________________|____________| Thyroid Gland | + + + A + + + + + + + + + + + + + + + + + + + + +| 48 | Follicular Cell, Adenoma | | 1 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Clitoral Gland | + M + A + + M + + + + + M + M + + + + + + + + + +| 37 | __________________________________________________________________________|____________| Ovary | + + + A + + + + + + + + + + + + + + + + + + + + +| 47 | Cystadenoma | | 1 | Granulosa Cell Tumor Benign | | 2 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Adenoma | | 1 | Deciduoma Benign | | 1 | Hemangioma | X X X | 5 | Lymphoma Malignant | X | 1 | Polyp Stromal | X | 2 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + A + + + + + + + + + + + + + + + + + + + + +| 48 | Lymphoma Malignant | X | 2 | __________________________________________________________________________|____________| Lymph Node | + | 6 | Lymphoma Malignant | | 3 | Iliac, Lymphoma Malignant | | 2 | Renal, Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Lymph Node, Bronchial | M + + M + + + + + + + + + + M + + M + + M + + M +| 36 | Hepatocellular Carcinoma, Metastatic,| | | Liver | X | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 16 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 5| 3| 5| 7| 6| 7| 6| 7| 7| 4| 7| 7| 4| 7| 7| 7| 6| 7| 7| 7| 6| 7| | DAY ON TEST | 3| 6| 3| 7| 5| 6| 3| 9| 3| 5| 3| 3| 1| 3| 3| 4| 3| 3| 3| 4| 3| 3| 3| 5| 3| | | 6| 5| 7| 9| 7| 9| 5| 3| 5| 6| 7| 4| 6| 7| 5| 1| 6| 5| 6| 6| 5| 4| 7| 3| 5| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A | 188 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Lymphoma Malignant | X | 3 | __________________________________________________________________________|____________| Lymph Node, Mandibular | M + + A M + M M + M + + + + + + + M + + + + + + +| 34 | Lymphoma Malignant | X | 2 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + A + + + + + + + + + + + + + + + + + + + + +| 46 | Lymphoma Malignant | X | 4 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | + + + A + + + M + M + + M M + M + + + + + + + + +| 37 | Hepatocellular Carcinoma, Metastatic,| | | Liver | X | 1 | Lymphoma Malignant | | 2 | __________________________________________________________________________|____________| Spleen | + + + A + + + + + + + + + + + + + + + + + + + + +| 48 | Hemangiosarcoma | | 1 | Lymphoma Malignant | X X | 7 | __________________________________________________________________________|____________| Thymus | + + + M + + + + + + M + + + M + + M + + + + + + +| 39 | Hepatocellular Carcinoma, Metastatic,| | | Liver | X | 1 | Lymphoma Malignant | X | 3 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | + + + A + + + + + + + + M + + + + + + + + + + + +| 48 | Carcinoma | X | 2 | __________________________________________________________________________|____________| Skin | + + + M + + + + + + + + M + + + + + + + + + + + +| 47 | Subcutaneous Tissue, Sarcoma | | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemangiosarcoma | | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant | X | 2 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + A + + + + + + + + + + + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Alveolar/Bronchiolar Adenoma | | 2 | Alveolar/Bronchiolar Adenoma, | | | Multiple | | 1 | Alveolar/Bronchiolar Carcinoma | | 3 | Hepatocellular Carcinoma, Metastatic,| | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 17 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 5| 3| 5| 7| 6| 7| 6| 7| 7| 4| 7| 7| 4| 7| 7| 7| 6| 7| 7| 7| 6| 7| | DAY ON TEST | 3| 6| 3| 7| 5| 6| 3| 9| 3| 5| 3| 3| 1| 3| 3| 4| 3| 3| 3| 4| 3| 3| 3| 5| 3| | | 6| 5| 7| 9| 7| 9| 5| 3| 5| 6| 7| 4| 6| 7| 5| 1| 6| 5| 6| 6| 5| 4| 7| 3| 5| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A | 188 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - cont | | | | | | Liver | X X | 5 | Lymphoma Malignant | X | 2 | Mediastinum, Hepatocellular | | | Carcinoma, Metastatic, Liver | X | 2 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Trachea | + + + A + + + + + + + + + + + + + + + + + + + + +| 48 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | + | 1 | __________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Adenoma | X X X | 6 | Adenoma, Multiple | | 1 | Carcinoma | X | 2 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + A + + + + + + + + + + + + + + + + + + + + +| 48 | Lymphoma Malignant | X | 3 | __________________________________________________________________________|____________| Urinary Bladder | + + + A + + + + I + + + + + + + + + + + + + + + +| 47 | Lymphoma Malignant | X | 1 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant | X X | 7 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 18 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 6| 7| 6| 7| 7| 6| 0| 7| 7| 5| 6| 6| 7| 7| 7| 4| 5| 6| 7| 7| 7| 7| 7| 6| 7| DAY ON TEST | 9| 3| 6| 3| 2| 5| 6| 3| 3| 4| 3| 0| 3| 3| 3| 9| 6| 6| 3| 3| 3| 1| 3| 0| 3| | 3| 5| 5| 4| 7| 3| 9| 4| 4| 8| 7| 2| 5| 4| 7| 8| 8| 5| 4| 5| 5| 6| 7| 9| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 375 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + I + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + M + + + + + + + + + + + + + + + + + + + + + +| Leiomyoma | | __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | M + + + + + + + + + + + + + + + + + + + + + I + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| Peyer's Patch, Lymphoma Malignant | X X | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + M + + + + + + + + + + + + + + + + + +| Peyer's Patch, Lymphoma Malignant | X X| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X | Hepatocellular Carcinoma | X X X X | Hepatocellular Adenoma | X X X X X X X X X| Hepatocellular Adenoma, Multiple | X X | Histiocytic Sarcoma | | Lymphoma Malignant | X | __________________________________________________________________________| Mesentery | + + + + | Hemangiosarcoma | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + M + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant | X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Blood Vessel | + | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Capsule, Adenoma | | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Pheochromocytoma Benign | | Bilateral, Pheochromocytoma Benign | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + M + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | M + + M M + + + + + + + M M + + + + + M + M + M M| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + M + + + + + + + + + + +| Pars Distalis, Adenoma | X X X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + I + + + + + + + + + + + + + + + +| Follicular Cell, Adenoma | | _____________________________________________________________________________________________________________________| Page 19 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 6| 7| 6| 7| 7| 6| 0| 7| 7| 5| 6| 6| 7| 7| 7| 4| 5| 6| 7| 7| 7| 7| 7| 6| 7| DAY ON TEST | 9| 3| 6| 3| 2| 5| 6| 3| 3| 4| 3| 0| 3| 3| 3| 9| 6| 6| 3| 3| 3| 1| 3| 0| 3| | 3| 5| 5| 4| 7| 3| 9| 4| 4| 8| 7| 2| 5| 4| 7| 8| 8| 5| 4| 5| 5| 6| 7| 9| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 375 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | M + + + + + + + M M + M M + + + + + + M + + + + +| __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant | | Polyp Stromal | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X | __________________________________________________________________________| Lymph Node | + + | Iliac, Lymphoma Malignant | | Pancreatic, Lymphoma Malignant | | Renal, Lymphoma Malignant | X | __________________________________________________________________________| Lymph Node, Bronchial | + + + I M + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + M + M + + + + M M + + + + + M + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + M + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant | X X X| __________________________________________________________________________| Lymph Node, Mediastinal | + + + + + + M M + M M + + + + M + M + + + + M + +| Carcinoma, Metastatic, Harderian | | Gland | X | Histiocytic Sarcoma | | Lymphoma Malignant | X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | | Histiocytic Sarcoma | | Lymphoma Malignant | X X | __________________________________________________________________________| Thymus | + M + + M + + + + + + + + + + + M + + + + + + I +| Lymphoma Malignant | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Carcinoma | X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Squamous Cell Carcinoma | X | Subcutaneous Tissue, Hemangiosarcoma | X | _____________________________________________________________________________________________________________________| Page 20 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 6| 7| 6| 7| 7| 6| 0| 7| 7| 5| 6| 6| 7| 7| 7| 4| 5| 6| 7| 7| 7| 7| 7| 6| 7| DAY ON TEST | 9| 3| 6| 3| 2| 5| 6| 3| 3| 4| 3| 0| 3| 3| 3| 9| 6| 6| 3| 3| 3| 1| 3| 0| 3| | 3| 5| 5| 4| 7| 3| 9| 4| 4| 8| 7| 2| 5| 4| 7| 8| 8| 5| 4| 5| 5| 6| 7| 9| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 375 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM - cont | | | | Subcutaneous Tissue, Sarcoma | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X | Osteosarcoma | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | __________________________________________________________________________| Spinal Cord | + | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X X | Alveolar/Bronchiolar Carcinoma | X X X X X | Carcinoma, Metastatic, Harderian | | Gland | X X | Histiocytic Sarcoma | | Osteosarcoma, Metastatic, Bone | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X X X X X X X X | Adenoma, Multiple | X | Carcinoma | X X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant | | Osteosarcoma, Metastatic, Bone | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + I + + +| Histiocytic Sarcoma | | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Lymphoma Malignant | X X X| _____________________________________________________________________________________________________________________| Page 21 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 6| 7| 7| 6| 7| 5| 6| 7| 7| 7| 7| 7| 6| 7| 2| 7| 7| 7| 7| 6| 7| 6| 7| 6| 7| | DAY ON TEST | 3| 3| 3| 0| 3| 7| 8| 2| 3| 3| 3| 3| 9| 3| 9| 3| 3| 3| 3| 7| 3| 4| 3| 3| 3| | | 1| 4| 7| 8| 7| 2| 0| 1| 5| 7| 6| 3| 3| 4| 6| 6| 4| 6| 7| 9| 7| 6| 7| 7| 7| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| A | 375 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Gallbladder | + + M A + A + + + + + + + + + + + + + + + + M + +| 45 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + A + A + + + + + + + + + + + + + + + + + + +| 47 | Leiomyoma | X | 1 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + A + A + + + + + + + + A + + + + + + + + + +| 47 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + A + A + + + + + + + + A + + + + + + + + + +| 47 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + A + A + + + + + + + + A + + + + + + + + + +| 45 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + A + A + + + + + + + + A + + + + + + + + + +| 47 | Peyer's Patch, Lymphoma Malignant | | 2 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + A + A + + + + + + + + A + + + + + + + + + +| 46 | Peyer's Patch, Lymphoma Malignant | | 2 | __________________________________________________________________________|____________| Liver | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | Hemangiosarcoma | X | 2 | Hepatocellular Carcinoma | X X X X| 8 | Hepatocellular Adenoma | X X X X | 13 | Hepatocellular Adenoma, Multiple | X X| 4 | Histiocytic Sarcoma | X X | 2 | Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Mesentery | + + + + | 8 | Hemangiosarcoma | X | 1 | __________________________________________________________________________|____________| Pancreas | + + + + + M + + + + + + + + + + + + + + + + + + +| 48 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant | X | 2 | __________________________________________________________________________|____________| Salivary Glands | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Stomach, Glandular | + + + A + A + + + + + + + + + + + + + + + + + + +| 48 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Blood Vessel | + | 2 | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Histiocytic Sarcoma | X | 1 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | + + + + + M + + + + + + + + + + + + + + + + + + +| 49 | Capsule, Adenoma | X | 1 | __________________________________________________________________________|____________| Adrenal Medulla | + + + + + M + + + + + + + + + + + + + + + + + + +| 49 | Pheochromocytoma Benign | X| 1 | Bilateral, Pheochromocytoma Benign | X | 1 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + M + M + + + + + + + + + + + + + + + + + + +| 47 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 22 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 6| 7| 7| 6| 7| 5| 6| 7| 7| 7| 7| 7| 6| 7| 2| 7| 7| 7| 7| 6| 7| 6| 7| 6| 7| | DAY ON TEST | 3| 3| 3| 0| 3| 7| 8| 2| 3| 3| 3| 3| 9| 3| 9| 3| 3| 3| 3| 7| 3| 4| 3| 3| 3| | | 1| 4| 7| 8| 7| 2| 0| 1| 5| 7| 6| 3| 3| 4| 6| 6| 4| 6| 7| 9| 7| 6| 7| 7| 7| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| A | 375 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Parathyroid Gland | + M + + + A M + M M M + + + + + + + M + + + M + +| 33 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + A + + + + + + + + + + + + + + + + + + +| 48 | Pars Distalis, Adenoma | X X X X X| 8 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + A + + + + + + + + + + + + + + + + + + +| 48 | Follicular Cell, Adenoma | X | 1 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Clitoral Gland | + + + + + M + + M + + M + M + + + + + + + + + + M| 39 | __________________________________________________________________________|____________| Ovary | + + + + + A + + + + + I + + + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Uterus | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant | X| 1 | Polyp Stromal | X | 1 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | Hemangiosarcoma | X | 2 | __________________________________________________________________________|____________| Lymph Node | + + +| 5 | Iliac, Lymphoma Malignant | X| 1 | Pancreatic, Lymphoma Malignant | X| 1 | Renal, Lymphoma Malignant | X| 2 | __________________________________________________________________________|____________| Lymph Node, Bronchial | M M + + + A + M + + + M + + M M + + + I + + + + +| 40 | Lymphoma Malignant | X| 2 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + M + + A M + M M + + + + + + M + + + M + + + +| 38 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant | X| 1 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + A + A + + + + + + + + + + M + + + + + + + +| 46 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant | X| 4 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | + + + M + A + + + M M + + M M + + + + + + M I + +| 35 | Carcinoma, Metastatic, Harderian | | | Gland | | 1 | Histiocytic Sarcoma | X | 1 | Lymphoma Malignant | X| 2 | __________________________________________________________________________|____________| Spleen | + + + + + M + + + + + + + + + + + + + + + + + + +| 49 | Hemangiosarcoma | X | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 23 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 6| 7| 7| 6| 7| 5| 6| 7| 7| 7| 7| 7| 6| 7| 2| 7| 7| 7| 7| 6| 7| 6| 7| 6| 7| | DAY ON TEST | 3| 3| 3| 0| 3| 7| 8| 2| 3| 3| 3| 3| 9| 3| 9| 3| 3| 3| 3| 7| 3| 4| 3| 3| 3| | | 1| 4| 7| 8| 7| 2| 0| 1| 5| 7| 6| 3| 3| 4| 6| 6| 4| 6| 7| 9| 7| 6| 7| 7| 7| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| A | 375 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Histiocytic Sarcoma | X X | 2 | Lymphoma Malignant | X X| 4 | __________________________________________________________________________|____________| Thymus | + + + + + A + M + + + M + + + M + + + + M + + + +| 41 | Lymphoma Malignant | X X X| 3 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | Adenoma | | 1 | Carcinoma | X | 2 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Squamous Cell Carcinoma | | 1 | Subcutaneous Tissue, Hemangiosarcoma | X | 2 | Subcutaneous Tissue, Sarcoma | | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemangiosarcoma | X | 2 | Osteosarcoma | X | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Spinal Cord | | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Lung | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | Alveolar/Bronchiolar Adenoma | | 2 | Alveolar/Bronchiolar Carcinoma | | 5 | Carcinoma, Metastatic, Harderian | | | Gland | | 2 | Histiocytic Sarcoma | X X | 2 | Osteosarcoma, Metastatic, Bone | X | 1 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Trachea | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | | 1 | __________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 24 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 6| 7| 7| 6| 7| 5| 6| 7| 7| 7| 7| 7| 6| 7| 2| 7| 7| 7| 7| 6| 7| 6| 7| 6| 7| | DAY ON TEST | 3| 3| 3| 0| 3| 7| 8| 2| 3| 3| 3| 3| 9| 3| 9| 3| 3| 3| 3| 7| 3| 4| 3| 3| 3| | | 1| 4| 7| 8| 7| 2| 0| 1| 5| 7| 6| 3| 3| 4| 6| 6| 4| 6| 7| 9| 7| 6| 7| 7| 7| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| A | 375 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ SPECIAL SENSES SYSTEM - cont | | | | | | Adenoma | X X X X X X | 15 | Adenoma, Multiple | | 1 | Carcinoma | X X | 4 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | Histiocytic Sarcoma | X X | 2 | Lymphoma Malignant | X | 1 | Osteosarcoma, Metastatic, Bone | X | 1 | __________________________________________________________________________|____________| Urinary Bladder | + + + A + A + + + + + + + + + + + + + + + + + + +| 47 | Histiocytic Sarcoma | X | 1 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Histiocytic Sarcoma | X X X | 3 | Lymphoma Malignant | X X X X| 7 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 25 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 7| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 7| 7| 7| 5| 7| DAY ON TEST | 1| 3| 0| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 2| 3| 3| 3| 3| 5| 3| | 6| 7| 6| 7| 5| 6| 6| 4| 8| 6| 6| 5| 7| 4| 1| 4| 5| 4| 6| 6| 4| 7| 4| 3| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 750 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + I + + + + + M + + M + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| Peyer's Patch, Lymphoma Malignant | | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| Peyer's Patch, Lymphoma Malignant | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Carcinoma | X X X X X X | Hepatocellular Carcinoma, Multiple | | Hepatocellular Adenoma | X X X X X X X X | Hepatocellular Adenoma, Multiple | X X X X X X| Lymphoma Malignant | X | __________________________________________________________________________| Mesentery | + + + + + + | Hemangioma | X | __________________________________________________________________________| Oral Mucosa | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Squamous Cell Papilloma | X X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Capsule, Adenoma | | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + I + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | __________________________________________________________________________| Parathyroid Gland | M + + + M + M + M + M + + + + M + + + + + + + M +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Pars Distalis, Adenoma | X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicular Cell, Adenoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | _____________________________________________________________________________________________________________________| Page 26 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 7| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 7| 7| 7| 5| 7| DAY ON TEST | 1| 3| 0| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 2| 3| 3| 3| 3| 5| 3| | 6| 7| 6| 7| 5| 6| 6| 4| 8| 6| 6| 5| 7| 4| 1| 4| 5| 4| 6| 6| 4| 7| 4| 3| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 750 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| GENITAL SYSTEM - cont | | | | | | __________________________________________________________________________| Clitoral Gland | + M + + I + + M + + + + + I + + I + M + + + + + +| __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + M + + +| Cystadenocarcinoma | | Cystadenoma | X X X | Luteoma | X | Teratoma Benign | | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | Polyp Stromal | X X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Sarcoma, Metastatic, Skin | X | __________________________________________________________________________| Lymph Node | + + + | Iliac, Lymphoma Malignant | | Renal, Lymphoma Malignant | | __________________________________________________________________________| Lymph Node, Bronchial | M + + M + + + + + + + + + + + M + M + + + + M M +| Lymphoma Malignant | X X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + M + M + + + + + + + + + + + M + +| __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | __________________________________________________________________________| Lymph Node, Mediastinal | + + + + + + M + M + + + + + + + M + + + M + + M +| Lymphoma Malignant | X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | X X | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + + + + + + + M + +| Lymphoma Malignant | X X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Sarcoma | X X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X X X | _____________________________________________________________________________________________________________________| Page 27 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 7| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 7| 7| 7| 5| 7| DAY ON TEST | 1| 3| 0| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 2| 3| 3| 3| 3| 5| 3| | 6| 7| 6| 7| 5| 6| 6| 4| 8| 6| 6| 5| 7| 4| 1| 4| 5| 4| 6| 6| 4| 7| 4| 3| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 750 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Alveolar/Bronchiolar Adenoma, | | Multiple | | Alveolar/Bronchiolar Carcinoma, | | Multiple | X X | Hepatocellular Carcinoma, Metastatic,| | Liver | | Lymphoma Malignant | X X | Sarcoma, Metastatic, Skin | X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X X X X X X X X X| Adenoma, Multiple | X | Carcinoma | X | Carcinoma, Multiple | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | X | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | X X X | _____________________________________________________________________________________________________________________| Page 28 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 7| 6| 6| 7| 6| 7| 0| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 3| 9| 5| 3| 0| 3| 5| 3| 2| 3| 3| 3| 3| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| | | 6| 6| 9| 7| 6| 3| 6| 1| 4| 9| 7| 7| 2| 6| 5| 7| 4| 5| 3| 4| 5| 6| 7| 7| 6| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| A | 750 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + M + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + A + + + + + + + + + + + + + + + + +| 46 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + A + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + A + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + A + A + + + + + + + + + + + + + + + + +| 48 | Peyer's Patch, Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + A + + + + + + + + + + + + + + + + +| 49 | Peyer's Patch, Lymphoma Malignant | X X | 2 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hepatocellular Carcinoma | X X X X X | 11 | Hepatocellular Carcinoma, Multiple | X | 1 | Hepatocellular Adenoma | X X X X X X X X X X X X X X| 22 | Hepatocellular Adenoma, Multiple | X X X X X X X | 13 | Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Mesentery | + + + + + | 11 | Hemangioma | | 1 | __________________________________________________________________________|____________| Oral Mucosa | + | 1 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Squamous Cell Papilloma | | 2 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant | | 1 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Capsule, Adenoma | X| 1 | __________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Adenoma | X | 1 | __________________________________________________________________________|____________| Parathyroid Gland | + + + + + + M + M + M + + M I M M M + + + + M + +| 34 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Pars Distalis, Adenoma | X X X | 4 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 29 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 7| 6| 6| 7| 6| 7| 0| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 3| 9| 5| 3| 0| 3| 5| 3| 2| 3| 3| 3| 3| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| | | 6| 6| 9| 7| 6| 3| 6| 1| 4| 9| 7| 7| 2| 6| 5| 7| 4| 5| 3| 4| 5| 6| 7| 7| 6| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| A | 750 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | Follicular Cell, Adenoma | X | 1 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Clitoral Gland | + + M + + + + + M + + M + M + + M + + M M + + + M| 36 | __________________________________________________________________________|____________| Ovary | + + + + M + + + + + + + + + + + + + + + + + + + +| 48 | Cystadenocarcinoma | X | 1 | Cystadenoma | | 3 | Luteoma | | 1 | Teratoma Benign | X | 1 | __________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Adenoma | X | 1 | Polyp Stromal | X | 3 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Sarcoma, Metastatic, Skin | | 1 | __________________________________________________________________________|____________| Lymph Node | + + + | 6 | Iliac, Lymphoma Malignant | X | 1 | Renal, Lymphoma Malignant | X X | 2 | __________________________________________________________________________|____________| Lymph Node, Bronchial | + + + + + + + M M + + + + M + + M + + + + + + + +| 40 | Lymphoma Malignant | X X | 4 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + M + M + + + + + + + + M + M +| 43 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + M + + + + + I + + + + + + + + + + +| 48 | Lymphoma Malignant | X X | 2 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | + + + + + + + + M + + M + + + + + + M + + + M + +| 41 | Lymphoma Malignant | X X | 3 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant | X X | 4 | __________________________________________________________________________|____________| Thymus | + + + + + + + M + + + + + + + + M + + + + + + + +| 47 | Lymphoma Malignant | X | 3 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Subcutaneous Tissue, Sarcoma | | 2 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 30 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 7| 6| 6| 7| 6| 7| 0| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 3| 9| 5| 3| 0| 3| 5| 3| 2| 3| 3| 3| 3| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| | | 6| 6| 9| 7| 6| 3| 6| 1| 4| 9| 7| 7| 2| 6| 5| 7| 4| 5| 3| 4| 5| 6| 7| 7| 6| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| A | 750 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ MUSCULOSKELETAL SYSTEM - cont | | | | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Alveolar/Bronchiolar Adenoma | X X X X X | 8 | Alveolar/Bronchiolar Adenoma, | | | Multiple | X | 1 | Alveolar/Bronchiolar Carcinoma, | | | Multiple | X | 3 | Hepatocellular Carcinoma, Metastatic,| | | Liver | X X X | 3 | Lymphoma Malignant | | 2 | Sarcoma, Metastatic, Skin | | 1 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Adenoma | X X X X X X | 16 | Adenoma, Multiple | X X | 3 | Carcinoma | X | 2 | Carcinoma, Multiple | | 1 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + A + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant | X X X X | 7 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 31 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 6| 4| 7| 5| 7| 7| 5| 7| 7| 7| 7| 7| 5| 7| DAY ON TEST | 3| 5| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 4| 3| 4| 3| 3| 0| 1| 3| 3| 3| 3| 0| 3| | 4| 3| 5| 6| 6| 5| 5| 4| 4| 4| 6| 3| 1| 6| 5| 5| 5| 3| 4| 4| 6| 7| 1| 3| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| CONTROL | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + A + + + A + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + M + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + A + + + + + + A + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + M + + + + A + + A + + + M + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + A + + A + + + A + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + A + + + A + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X | Hepatocellular Carcinoma | X X X X X | Hepatocellular Carcinoma, Multiple | X X | Hepatocellular Adenoma | X X X X X | Hepatocellular Adenoma, Multiple | X X X X X | Hepatocholangiocarcinoma, Multiple | X | Lymphoma Malignant | | __________________________________________________________________________| Mesentery | + + | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | __________________________________________________________________________| Tooth | + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Carcinoma, Metastatic,| | Liver | X | Lymphoma Malignant | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Capsule, Adenoma | X X| __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | __________________________________________________________________________| Parathyroid Gland | + + + + + + M + + M + + + + + + + + + M + M M + M| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + M + + + + + M + +| Pars Distalis, Adenoma | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicular Cell, Adenoma | X | _____________________________________________________________________________________________________________________| Page 32 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 6| 4| 7| 5| 7| 7| 5| 7| 7| 7| 7| 7| 5| 7| DAY ON TEST | 3| 5| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 4| 3| 4| 3| 3| 0| 1| 3| 3| 3| 3| 0| 3| | 4| 3| 5| 6| 6| 5| 5| 4| 4| 4| 6| 3| 1| 6| 5| 5| 5| 3| 4| 4| 6| 7| 1| 3| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| CONTROL | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant | | __________________________________________________________________________| Penis | | __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + + M + + + + + +| __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + M + + + + + + + + + +| Lymphoma Malignant | | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | Interstitial Cell, Adenoma | X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X | __________________________________________________________________________| Lymph Node | | Lymphoma Malignant | | Iliac, Lymphoma Malignant | | Renal, Lymphoma Malignant | | __________________________________________________________________________| Lymph Node, Bronchial | + M + M + + + M + + + + M M + M M + M + M + + + +| Lymphoma Malignant | | __________________________________________________________________________| Lymph Node, Mandibular | M M + + M + + M + + + M + + + + + + + + + + + + +| Lymphoma Malignant | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + M + + + + + +| Lymphoma Malignant | | __________________________________________________________________________| Lymph Node, Mediastinal | + M + + M + M + + + M M + + M M M + + M + + M + M| Lymphoma Malignant | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X | Lymphoma Malignant | | __________________________________________________________________________| Thymus | + M M + + + I M + + M + + M M + + + M + + M + M +| Lymphoma Malignant | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | M + + + + + + + + + + + + + + + + + + + + + + + +| Prepuce, Histiocytic Sarcoma | | Subcutaneous Tissue, Histiocytic | | Sarcoma | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | _____________________________________________________________________________________________________________________| Page 33 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 6| 4| 7| 5| 7| 7| 5| 7| 7| 7| 7| 7| 5| 7| DAY ON TEST | 3| 5| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 4| 3| 4| 3| 3| 0| 1| 3| 3| 3| 3| 0| 3| | 4| 3| 5| 6| 6| 5| 5| 4| 4| 4| 6| 3| 1| 6| 5| 5| 5| 3| 4| 4| 6| 7| 1| 3| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| CONTROL | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM - cont | | | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X X | Alveolar/Bronchiolar Adenoma, | | Multiple | X X | Alveolar/Bronchiolar Carcinoma | X | Hepatocellular Carcinoma, Metastatic,| | Liver | X X X | Lymphoma Malignant | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X X X X| Carcinoma | X | Lymphoma Malignant | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant | | _____________________________________________________________________________________________________________________| Page 34 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 4| 7| 5| 7| 7| 6| 7| 7| 6| 7| 7| 4| 7| 7| 6| 6| 5| 6| 7| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 9| 3| 7| 3| 3| 0| 3| 3| 5| 3| 0| 4| 3| 3| 6| 2| 8| 5| 3| 3| | | 5| 4| 7| 4| 4| 0| 5| 6| 6| 4| 9| 5| 4| 3| 7| 1| 9| 6| 4| 5| 3| 1| 3| 5| 4| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | CONTROL | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + M + + + + + + M + + + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Gallbladder | I + + + + A + M + + A + M + + + + + + + + + + + +| 43 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + A + + + + A + + + + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + A + + + + A + + + + + + + + + + + + + +| 47 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + A + + + + A + + + + + + + + + + + + + +| 46 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + A + + + + M + + + + + + + + + + + + + +| 44 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + A + + + + A + + + + + + + + + + + + + +| 45 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + A + + + + A + + + + + + + + + + + + + +| 46 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemangiosarcoma | | 1 | Hepatocellular Carcinoma | X X X X X X | 11 | Hepatocellular Carcinoma, Multiple | X X X | 5 | Hepatocellular Adenoma | X X X X X | 10 | Hepatocellular Adenoma, Multiple | X X | 7 | Hepatocholangiocarcinoma, Multiple | | 1 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Mesentery | + | 3 | __________________________________________________________________________|____________| Pancreas | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + A + + + + A + + + + + + + + + + + + + +| 48 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Tooth | + + | 3 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hepatocellular Carcinoma, Metastatic,| | | Liver | | 1 | Lymphoma Malignant | X | 1 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | Capsule, Adenoma | X X X | 5 | __________________________________________________________________________|____________| Adrenal Medulla | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | Adenoma | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 35 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 4| 7| 5| 7| 7| 6| 7| 7| 6| 7| 7| 4| 7| 7| 6| 6| 5| 6| 7| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 9| 3| 7| 3| 3| 0| 3| 3| 5| 3| 0| 4| 3| 3| 6| 2| 8| 5| 3| 3| | | 5| 4| 7| 4| 4| 0| 5| 6| 6| 4| 9| 5| 4| 3| 7| 1| 9| 6| 4| 5| 3| 1| 3| 5| 4| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | CONTROL | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Parathyroid Gland | M + + + + + + + + + + + + + M M + + + + + + + + +| 41 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + I + + + + + + + + + + + + + + +| 47 | Pars Distalis, Adenoma | X | 1 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Follicular Cell, Adenoma | | 1 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Penis | + | 1 | __________________________________________________________________________|____________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Seminal Vesicle | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant | X | 1 | Interstitial Cell, Adenoma | X | 2 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | Hemangiosarcoma | | 1 | __________________________________________________________________________|____________| Lymph Node | + + + | 3 | Lymphoma Malignant | X | 1 | Iliac, Lymphoma Malignant | X | 1 | Renal, Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Lymph Node, Bronchial | + M M M M A M M M + + M M + + + + M M + M + + M M| 26 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + M + + + + + + + + + + M + + M + M M + +| 40 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + M + + + + + + + + + M + + + +| 47 | Lymphoma Malignant | X X | 2 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | + M + + + A + + + + + + + + + + M + + + + M + + +| 35 | Lymphoma Malignant | X X | 2 | __________________________________________________________________________|____________| Spleen | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 36 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 4| 7| 5| 7| 7| 6| 7| 7| 6| 7| 7| 4| 7| 7| 6| 6| 5| 6| 7| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 9| 3| 7| 3| 3| 0| 3| 3| 5| 3| 0| 4| 3| 3| 6| 2| 8| 5| 3| 3| | | 5| 4| 7| 4| 4| 0| 5| 6| 6| 4| 9| 5| 4| 3| 7| 1| 9| 6| 4| 5| 3| 1| 3| 5| 4| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | CONTROL | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Hemangiosarcoma | | 1 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Thymus | + + + + I M + + M + M M + + + + + M + + M + + + +| 33 | Lymphoma Malignant | X | 1 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Prepuce, Histiocytic Sarcoma | X | 1 | Subcutaneous Tissue, Histiocytic | | | Sarcoma | X X | 2 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Alveolar/Bronchiolar Adenoma | X X X X X X X | 9 | Alveolar/Bronchiolar Adenoma, | | | Multiple | | 2 | Alveolar/Bronchiolar Carcinoma | X | 2 | Hepatocellular Carcinoma, Metastatic,| | | Liver | X X X | 6 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Trachea | + + + + + A + + + + + + + + + + + + + + + + + + +| 49 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Adenoma | X X X X | 9 | Carcinoma | | 1 | Lymphoma Malignant | X | 1 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 37 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 4| 7| 5| 7| 7| 6| 7| 7| 6| 7| 7| 4| 7| 7| 6| 6| 5| 6| 7| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 9| 3| 7| 3| 3| 0| 3| 3| 5| 3| 0| 4| 3| 3| 6| 2| 8| 5| 3| 3| | | 5| 4| 7| 4| 4| 0| 5| 6| 6| 4| 9| 5| 4| 3| 7| 1| 9| 6| 4| 5| 3| 1| 3| 5| 4| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | CONTROL | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ URINARY SYSTEM - cont | | | | | | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + A + + + + A + + + + + + + + + + + + + +| 48 | Lymphoma Malignant | X | 1 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Histiocytic Sarcoma | X X | 3 | Lymphoma Malignant | X X X | 3 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 38 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 3| 3| 2| 3| 3| 3| 3| 8| 3| 3| 3| 4| 3| 3| 3| 2| 3| 2| 3| 3| 3| 3| 3| | 4| 6| 5| 6| 1| 4| 5| 4| 6| 4| 5| 5| 6| 8| 7| 5| 5| 8| 6| 1| 4| 7| 7| 1| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 188 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + A + + + + + + + + + + + + + A +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + A + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + A + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + A + + + + + + + + + + + + + A +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + A + + + + + + + + + + + + + A +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + A + + + + + + + + + + + + + + +| Peyer's Patch, Lymphoma Malignant | X | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + A + + + + + + + + + + + + + A +| Lymphoma Malignant | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X X | Hepatocellular Carcinoma | X X X X X X X X | Hepatocellular Carcinoma, Multiple | | Hepatocellular Adenoma | X X X X | Hepatocellular Adenoma, Multiple | X X | Lymphoma Malignant | | Squamous Cell Carcinoma, Metastatic, | | Stomach, Forestomach | X | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Lymphoma Malignant | X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Squamous Cell Carcinoma | X | Squamous Cell Papilloma | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + A + + + + + + + + + + + + + A +| __________________________________________________________________________| Tooth | + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Capsule, Adenoma | X | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X | __________________________________________________________________________| Parathyroid Gland | + + M + + + M + + M M + + + + I + + M + + M + + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicular Cell, Carcinoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| Page 39 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 3| 3| 2| 3| 3| 3| 3| 8| 3| 3| 3| 4| 3| 3| 3| 2| 3| 2| 3| 3| 3| 3| 3| | 4| 6| 5| 6| 1| 4| 5| 4| 6| 4| 5| 5| 6| 8| 7| 5| 5| 8| 6| 1| 4| 7| 7| 1| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 188 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Penis | + | __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + A + + + + + + + + + + + + + + +| __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Interstitial Cell, Adenoma | X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X | __________________________________________________________________________| Lymph Node | + | Renal, Lymphoma Malignant | X | __________________________________________________________________________| Lymph Node, Bronchial | M + + I M + + + M + M M + + + M M M + M + M + + M| Lymphoma Malignant | | __________________________________________________________________________| Lymph Node, Mandibular | + M + + + + + M + + + + + M + + M + + + + + + + +| __________________________________________________________________________| Lymph Node, Mesenteric | + + + + M + + M + + + + + + + + + + + + + + + + +| Lymphoma Malignant | X X | __________________________________________________________________________| Lymph Node, Mediastinal | + + M + + M + + + M + + M + + + + + + + + + + + M| Lymphoma Malignant | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | | Lymphoma Malignant | X X | __________________________________________________________________________| Thymus | + M + + + + + + M M M + M + + + M M + + + + M + M| Lymphoma Malignant | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + M + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + + + + + + + A +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X X X X X X | Alveolar/Bronchiolar Carcinoma | X X | Hepatocellular Carcinoma, Metastatic,| | Liver | X | Lymphoma Malignant | | _____________________________________________________________________________________________________________________| Page 40 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 3| 3| 2| 3| 3| 3| 3| 8| 3| 3| 3| 4| 3| 3| 3| 2| 3| 2| 3| 3| 3| 3| 3| | 4| 6| 5| 6| 1| 4| 5| 4| 6| 4| 5| 5| 6| 8| 7| 5| 5| 8| 6| 1| 4| 7| 7| 1| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 188 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + A +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + A +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X X | Adenoma, Multiple | X X | Carcinoma | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | | __________________________________________________________________________| Urethra | + | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Lymphoma Malignant | X X | _____________________________________________________________________________________________________________________| Page 41 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 6| 4| 7| 3| 7| 5| 7| 7| 7| 6| 7| 7| | DAY ON TEST | 6| 3| 3| 3| 3| 3| 0| 3| 3| 7| 3| 3| 3| 5| 7| 3| 2| 3| 7| 3| 3| 3| 4| 3| 3| | | 5| 5| 5| 7| 5| 6| 7| 5| 5| 9| 7| 4| 6| 9| 9| 5| 0| 7| 8| 4| 7| 6| 6| 5| 6| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A | 188 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + + + + + M + + + +| 47 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Peyer's Patch, Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemangiosarcoma | | 2 | Hepatocellular Carcinoma | X X X X | 12 | Hepatocellular Carcinoma, Multiple | X X | 2 | Hepatocellular Adenoma | X X X X X X | 10 | Hepatocellular Adenoma, Multiple | X X | 4 | Lymphoma Malignant | X | 1 | Squamous Cell Carcinoma, Metastatic, | | | Stomach, Forestomach | | 1 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Adenoma | | 1 | Lymphoma Malignant | X | 2 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + M + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Squamous Cell Carcinoma | | 1 | Squamous Cell Papilloma | X| 1 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Tooth | + + | 3 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Capsule, Adenoma | | 1 | __________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Adenoma | X | 3 | __________________________________________________________________________|____________| Parathyroid Gland | M + + + + + + M + M M + + + + M + M M + + + M + M| 34 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 42 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 6| 4| 7| 3| 7| 5| 7| 7| 7| 6| 7| 7| | DAY ON TEST | 6| 3| 3| 3| 3| 3| 0| 3| 3| 7| 3| 3| 3| 5| 7| 3| 2| 3| 7| 3| 3| 3| 4| 3| 3| | | 5| 5| 5| 7| 5| 6| 7| 5| 5| 9| 7| 4| 6| 9| 9| 5| 0| 7| 8| 4| 7| 6| 6| 5| 6| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A | 188 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Follicular Cell, Carcinoma | | 1 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Penis | + | 2 | __________________________________________________________________________|____________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Interstitial Cell, Adenoma | | 1 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemangiosarcoma | X | 2 | __________________________________________________________________________|____________| Lymph Node | | 1 | Renal, Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Lymph Node, Bronchial | + M + M M + M M + + M + + + M M M M + + + M + + M| 26 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + M + M M M + + + + + M M M| 39 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | M + + + + + + + + + + M + + + + + + + + + + + + +| 46 | Lymphoma Malignant | X X | 4 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | + + M + + M + + + + + + + + + M M M M + + M + M +| 37 | Lymphoma Malignant | X X | 2 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemangiosarcoma | X | 1 | Lymphoma Malignant | X X | 4 | __________________________________________________________________________|____________| Thymus | + + + + + + M + + + + + + M M + + + M M + + M + M| 34 | Lymphoma Malignant | X | 1 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 43 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 6| 4| 7| 3| 7| 5| 7| 7| 7| 6| 7| 7| | DAY ON TEST | 6| 3| 3| 3| 3| 3| 0| 3| 3| 7| 3| 3| 3| 5| 7| 3| 2| 3| 7| 3| 3| 3| 4| 3| 3| | | 5| 5| 5| 7| 5| 6| 7| 5| 5| 9| 7| 4| 6| 9| 9| 5| 0| 7| 8| 4| 7| 6| 6| 5| 6| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A | 188 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ MUSCULOSKELETAL SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Alveolar/Bronchiolar Adenoma | X X X X | 10 | Alveolar/Bronchiolar Carcinoma | X | 3 | Hepatocellular Carcinoma, Metastatic,| | | Liver | | 1 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | + | 1 | __________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Adenoma | X X X | 6 | Adenoma, Multiple | X X | 4 | Carcinoma | | 1 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Urethra | | 1 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Lymphoma Malignant | X X | 4 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 44 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 7| 5| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 4| 7| 7| DAY ON TEST | 3| 3| 3| 3| 9| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 3| 0| 3| 9| 3| 3| | 5| 4| 6| 5| 3| 6| 4| 3| 7| 4| 7| 6| 7| 6| 6| 7| 4| 6| 1| 5| 5| 4| 8| 6| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 375 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + M + + + + + + + + + A + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + A + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + M + + + + + + + + + + A + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + A + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + A + A + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + A + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + A + + + + + +| Peyer's Patch, Lymphoma Malignant | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X X | Hepatocellular Carcinoma | X X X | Hepatocellular Carcinoma, Multiple | | Hepatocellular Adenoma | X X X X X X X | Hepatocellular Adenoma, Multiple | | Histiocytic Sarcoma | X X | Lymphoma Malignant | | __________________________________________________________________________| Mesentery | + + + | Fat, Histiocytic Sarcoma | X | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + A + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + A + + + + + +| Histiocytic Sarcoma | | __________________________________________________________________________| Tooth | + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + M + + + + + + + + + + + + + + + + +| Bilateral, Capsule, Adenoma | | Capsule, Adenoma | X | __________________________________________________________________________| Adrenal Medulla | + + + + + + + M + + + + + + + + + + + + + + + + +| Pheochromocytoma Complex | X | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + A + + + + + +| __________________________________________________________________________| Parathyroid Gland | + + + M + + M + + + + + + + + + M + M + M + M + +| __________________________________________________________________________| Pituitary Gland | + + + + I + + + + + + + + + + + + + + + + + M + +| Lymphoma Malignant | | Pars Intermedia, Adenoma | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + A + + + + + +| _____________________________________________________________________________________________________________________| Page 45 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 7| 5| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 4| 7| 7| DAY ON TEST | 3| 3| 3| 3| 9| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 3| 0| 3| 9| 3| 3| | 5| 4| 6| 5| 3| 6| 4| 3| 7| 4| 7| 6| 7| 6| 6| 7| 4| 6| 1| 5| 5| 4| 8| 6| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 375 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Penis | + | __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + M + + + +| __________________________________________________________________________| Prostate | + + + + + + + + + + M + + + + + + + A + + + + + +| Histiocytic Sarcoma | | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + M + + + + + +| Histiocytic Sarcoma | | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Interstitial Cell, Adenoma | X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node | | __________________________________________________________________________| Lymph Node, Bronchial | M M M M M M M M M + + M + M M M + M + M + M M M M| Histiocytic Sarcoma | | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + M M + + + + + + M + + + + + + M + +| __________________________________________________________________________| Lymph Node, Mesenteric | + M + + + + + M + + + + + + + + + + M + + + + + +| Histiocytic Sarcoma | X | Lymphoma Malignant | | __________________________________________________________________________| Lymph Node, Mediastinal | + + + + + + + + + + + + + + + M + + M M + + + + +| Histiocytic Sarcoma | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | X | Histiocytic Sarcoma | X | __________________________________________________________________________| Thymus | + M + M M I M I + + + + + + + + + + + M M + M M +| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Hemangiosarcoma | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Harderian | | Gland | | Lymphoma Malignant | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | _____________________________________________________________________________________________________________________| Page 46 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 7| 5| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 4| 7| 7| DAY ON TEST | 3| 3| 3| 3| 9| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 3| 0| 3| 9| 3| 3| | 5| 4| 6| 5| 3| 6| 4| 3| 7| 4| 7| 6| 7| 6| 6| 7| 4| 6| 1| 5| 5| 4| 8| 6| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 375 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | __________________________________________________________________________| Larynx | + + + + + + + + + + + + + + + + + + A + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X X X X| Alveolar/Bronchiolar Adenoma, | | Multiple | X | Alveolar/Bronchiolar Carcinoma | X X | Carcinoma, Metastatic, Harderian | | Gland | | Hepatocellular Carcinoma, Metastatic,| | Liver | X | Histiocytic Sarcoma | X | Mediastinum, Histiocytic Sarcoma | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Harderian | | Gland | | Olfactory Epithelium, Lymphoma | | Malignant | | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + A + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X X X X X X X X X | Adenoma, Multiple | | Carcinoma | X | Lymphoma Malignant | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + A + + + + + +| Renal Tubule, Adenoma | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + A + + + + + +| _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X | Lymphoma Malignant | | _____________________________________________________________________________________________________________________| Page 47 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 5| 7| 5| 7| 4| 5| 7| 7| 3| 5| 5| 7| 7| 3| 7| 7| 7| 5| 6| 6| 7| 6| | DAY ON TEST | 3| 3| 0| 8| 3| 9| 3| 3| 6| 3| 3| 3| 7| 8| 3| 3| 7| 3| 3| 2| 2| 4| 5| 3| 7| | | 6| 6| 7| 1| 7| 2| 5| 6| 3| 4| 4| 1| 6| 1| 7| 7| 7| 6| 7| 6| 0| 5| 5| 7| 9| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| A | 375 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + I + + + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Gallbladder | + + M + + + + M + + M + + + + + M + + I + + + + +| 43 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + A + + + + + + + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + A + +| 47 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + A + + + + + + + + + + + + + + A + +| 47 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + A + + + + + + + + + + + + + + A + +| 46 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + A + + + + + + + + + + + + + + A + +| 47 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + A + + + + A + + + + + + + + + + + + + + A + +| 46 | Peyer's Patch, Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemangiosarcoma | | 2 | Hepatocellular Carcinoma | X X X X X | 8 | Hepatocellular Carcinoma, Multiple | X X | 2 | Hepatocellular Adenoma | X X X X X | 12 | Hepatocellular Adenoma, Multiple | X | 1 | Histiocytic Sarcoma | X | 3 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Mesentery | + + + | 6 | Fat, Histiocytic Sarcoma | X | 2 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Histiocytic Sarcoma | X | 1 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + A + + + + + + + + + + + + + + + + +| 48 | Histiocytic Sarcoma | X | 1 | __________________________________________________________________________|____________| Tooth | | 1 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Bilateral, Capsule, Adenoma | X | 1 | Capsule, Adenoma | | 1 | __________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Pheochromocytoma Complex | | 1 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 48 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 5| 7| 5| 7| 4| 5| 7| 7| 3| 5| 5| 7| 7| 3| 7| 7| 7| 5| 6| 6| 7| 6| | DAY ON TEST | 3| 3| 0| 8| 3| 9| 3| 3| 6| 3| 3| 3| 7| 8| 3| 3| 7| 3| 3| 2| 2| 4| 5| 3| 7| | | 6| 6| 7| 1| 7| 2| 5| 6| 3| 4| 4| 1| 6| 1| 7| 7| 7| 6| 7| 6| 0| 5| 5| 7| 9| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| A | 375 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Parathyroid Gland | + M + + M + + + + + + + M M + + + + + + M + + M +| 38 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + I + M + + + + +| 46 | Lymphoma Malignant | X | 1 | Pars Intermedia, Adenoma | X | 1 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Penis | + + | 3 | __________________________________________________________________________|____________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + + + + + + + M + + +| 47 | Histiocytic Sarcoma | X | 1 | __________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Histiocytic Sarcoma | X | 1 | __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Interstitial Cell, Adenoma | | 1 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Lymph Node | + | 1 | __________________________________________________________________________|____________| Lymph Node, Bronchial | M M + M + + + + + M + + + + + + + + M + + M + M +| 24 | Histiocytic Sarcoma | X | 1 | __________________________________________________________________________|____________| Lymph Node, Mandibular | M M M + M + M + + + M M + + + + + + + + + M M + +| 37 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| 47 | Histiocytic Sarcoma | X | 2 | Lymphoma Malignant | X | 1 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | + + M M + + + M + + + M M M + + + M + + M M M + M| 36 | Histiocytic Sarcoma | X | 1 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemangiosarcoma | | 1 | Histiocytic Sarcoma | | 1 | __________________________________________________________________________|____________| Thymus | M + M + + M + + + M M + + + + M + + + + + M + + +| 33 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| | __________________________________________________________________________|____________| Skin | + + + + + + I + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 49 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 5| 7| 5| 7| 4| 5| 7| 7| 3| 5| 5| 7| 7| 3| 7| 7| 7| 5| 6| 6| 7| 6| | DAY ON TEST | 3| 3| 0| 8| 3| 9| 3| 3| 6| 3| 3| 3| 7| 8| 3| 3| 7| 3| 3| 2| 2| 4| 5| 3| 7| | | 6| 6| 7| 1| 7| 2| 5| 6| 3| 4| 4| 1| 6| 1| 7| 7| 7| 6| 7| 6| 0| 5| 5| 7| 9| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| A | 375 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ INTEGUMENTARY SYSTEM - cont | | | | | | Subcutaneous Tissue, Hemangiosarcoma | | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Carcinoma, Metastatic, Harderian | | | Gland | X| 1 | Lymphoma Malignant | X | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Alveolar/Bronchiolar Adenoma | X X X X | 8 | Alveolar/Bronchiolar Adenoma, | | | Multiple | | 1 | Alveolar/Bronchiolar Carcinoma | X | 3 | Carcinoma, Metastatic, Harderian | | | Gland | X| 1 | Hepatocellular Carcinoma, Metastatic,| | | Liver | X X X | 4 | Histiocytic Sarcoma | | 1 | Mediastinum, Histiocytic Sarcoma | X | 1 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Carcinoma, Metastatic, Harderian | | | Gland | X| 1 | Olfactory Epithelium, Lymphoma | | | Malignant | X | 1 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | + + | 3 | __________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Adenoma | X X X X X X X X | 18 | Adenoma, Multiple | X | 1 | Carcinoma | X X X X X| 6 | Lymphoma Malignant | X | 1 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 50 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 5| 7| 5| 7| 4| 5| 7| 7| 3| 5| 5| 7| 7| 3| 7| 7| 7| 5| 6| 6| 7| 6| | DAY ON TEST | 3| 3| 0| 8| 3| 9| 3| 3| 6| 3| 3| 3| 7| 8| 3| 3| 7| 3| 3| 2| 2| 4| 5| 3| 7| | | 6| 6| 7| 1| 7| 2| 5| 6| 3| 4| 4| 1| 6| 1| 7| 7| 7| 6| 7| 6| 0| 5| 5| 7| 9| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| A | 375 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ URINARY SYSTEM - cont | | | | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Renal Tubule, Adenoma | X | 1 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Histiocytic Sarcoma | X | 3 | Lymphoma Malignant | X X | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 51 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 6| 6| 7| 7| 6| 6| 6| 7| 6| 7| 7| 7| 7| 4| 6| 6| 7| 7| 7| 6| 6| 7| 7| 7| 7| DAY ON TEST | 6| 7| 3| 3| 4| 7| 3| 3| 5| 3| 3| 3| 3| 0| 7| 0| 3| 3| 3| 9| 9| 3| 3| 3| 3| | 5| 9| 4| 5| 9| 9| 2| 4| 9| 5| 7| 6| 7| 6| 1| 9| 7| 5| 4| 9| 9| 5| 7| 6| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 750 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + M + A + + + + + I + + A A + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + A + + + + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + I + A + + + + + + + + A I + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + A + + + + + + + + A A + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + A + + + + + + + + A A + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + A + + + + + + + + A A + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + A + + + + + + + + A A + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Carcinoma | X X X X X | Hepatocellular Carcinoma, Multiple | X | Hepatocellular Adenoma | X X X X | Hepatocellular Adenoma, Multiple | X X X X X | Lymphoma Malignant | X | __________________________________________________________________________| Mesentery | + + | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + A + + + + + + + + A + + + + + + + + + + +| Squamous Cell Papilloma | | __________________________________________________________________________| Stomach, Glandular | + + + + A + + + + + + + + A + + + + + + + + + + +| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + M + + + + + + + + + + +| Adenoma | X | Lymphoma Malignant | X | Capsule, Adenoma | X | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + M + + + + + + + + + + +| Pheochromocytoma Benign | X | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + A + + + + + + + + + + +| Adenoma | X | __________________________________________________________________________| Parathyroid Gland | + M + + + + + + M + M + + + + M + + + M M + + + +| __________________________________________________________________________| Pituitary Gland | I + + + + + + + + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Thyroid Gland | + + + + + + + + M + + + + A + + + + + + + + + + +| Follicular Cell, Adenoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Penis | | _____________________________________________________________________________________________________________________| Page 52 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 6| 6| 7| 7| 6| 6| 6| 7| 6| 7| 7| 7| 7| 4| 6| 6| 7| 7| 7| 6| 6| 7| 7| 7| 7| DAY ON TEST | 6| 7| 3| 3| 4| 7| 3| 3| 5| 3| 3| 3| 3| 0| 7| 0| 3| 3| 3| 9| 9| 3| 3| 3| 3| | 5| 9| 4| 5| 9| 9| 2| 4| 9| 5| 7| 6| 7| 6| 1| 9| 7| 5| 4| 9| 9| 5| 7| 6| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 750 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| GENITAL SYSTEM - cont | | | | __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Prostate | + + + + + + + + + + + + + M + + + + + + + + + + +| __________________________________________________________________________| Seminal Vesicle | + + + + A + + + + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Interstitial Cell, Adenoma | X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node | | __________________________________________________________________________| Lymph Node, Bronchial | M + + + + + + M + + + + M M M + + M M + + + M + M| Lymphoma Malignant | X | __________________________________________________________________________| Lymph Node, Mandibular | + + M + + + I + + + + + + A + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + A + + + + + + + + + + +| Histiocytic Sarcoma | X| Lymphoma Malignant | X | __________________________________________________________________________| Lymph Node, Mediastinal | + M M + + + + M + + M + + + M M + M + + + + + M +| __________________________________________________________________________| Spleen | + + + + + + + + + + + + + A + + + + + + + + + + +| Histiocytic Sarcoma | X| Lymphoma Malignant | X | __________________________________________________________________________| Thymus | + + + + + + M + + M + + + + M + + M + + + + + M +| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + A + + + + + + + + + + +| Lymphoma Malignant | X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Larynx | + + + + + + + + M + + + + A + + + + + + + + + + +| __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X X X X | Alveolar/Bronchiolar Adenoma, | | Multiple | | Alveolar/Bronchiolar Carcinoma | X X X | Alveolar/Bronchiolar Carcinoma, | | Multiple | X | Carcinoma, Metastatic, Harderian | | Gland | X | Hepatocellular Carcinoma, Metastatic,| | _____________________________________________________________________________________________________________________| Page 53 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 _____________________________________________________________________________________________________________________ | 6| 6| 7| 7| 6| 6| 6| 7| 6| 7| 7| 7| 7| 4| 6| 6| 7| 7| 7| 6| 6| 7| 7| 7| 7| DAY ON TEST | 6| 7| 3| 3| 4| 7| 3| 3| 5| 3| 3| 3| 3| 0| 7| 0| 3| 3| 3| 9| 9| 3| 3| 3| 3| | 5| 9| 4| 5| 9| 9| 2| 4| 9| 5| 7| 6| 7| 6| 1| 9| 7| 5| 4| 9| 9| 5| 7| 6| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 750 PPM | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Liver | X | Lymphoma Malignant | X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Harderian | | Gland | X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + A + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + + + + | __________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + A + + + + + + + + + + +| Adenoma | X X X X X X X X X X X X| Adenoma, Multiple | X X X | Carcinoma | X X | Carcinoma, Multiple | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + A + + + + + + + + + + +| Lymphoma Malignant | X | __________________________________________________________________________| Ureter | | __________________________________________________________________________| Urinary Bladder | + + + + A + + + + + + + + A + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X| Lymphoma Malignant | X | _____________________________________________________________________________________________________________________| Page 54 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 3| 7| 7| 5| 7| 7| 7| 7| 5| 7| 7| 7| 4| 5| 5| 7| 7| 6| 7| 6| 7| 7| | DAY ON TEST | 3| 3| 3| 9| 3| 0| 9| 2| 3| 3| 3| 9| 3| 3| 3| 9| 5| 8| 3| 3| 9| 3| 8| 3| 3| | | 4| 5| 5| 3| 4| 4| 5| 6| 7| 7| 4| 6| 6| 7| 6| 7| 1| 6| 4| 7| 9| 7| 8| 4| 6| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| A | 750 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Gallbladder | + + + + + A + + + I + + + + + + + + + + + + + I +| 42 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| 46 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + A + + + + + + + + + + + + + + + + + + +| 46 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + A + + + + + M + + + + + + + + + + + + +| 45 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + A + + + + + + + + + + + + +| 46 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + A + + + + + A + + + + + + + + + + + + +| 45 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hepatocellular Carcinoma | X | 6 | Hepatocellular Carcinoma, Multiple | X X | 3 | Hepatocellular Adenoma | X X X X X X X | 11 | Hepatocellular Adenoma, Multiple | X| 6 | Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Mesentery | | 2 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | Squamous Cell Papilloma | X | 1 | __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + I + + + + +| 48 | Adenoma | X | 2 | Lymphoma Malignant | | 1 | Capsule, Adenoma | X X | 3 | __________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + M + + + M + + + + +| 47 | Pheochromocytoma Benign | | 1 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Adenoma | | 1 | __________________________________________________________________________|____________| Parathyroid Gland | M M M + + M + + + + + + + + M M + + + + + M + + +| 37 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + I + + + + + + + + + + + + + + + + + + +| 47 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | Follicular Cell, Adenoma | X | 1 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 55 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 3| 7| 7| 5| 7| 7| 7| 7| 5| 7| 7| 7| 4| 5| 5| 7| 7| 6| 7| 6| 7| 7| | DAY ON TEST | 3| 3| 3| 9| 3| 0| 9| 2| 3| 3| 3| 9| 3| 3| 3| 9| 5| 8| 3| 3| 9| 3| 8| 3| 3| | | 4| 5| 5| 3| 4| 4| 5| 6| 7| 7| 4| 6| 6| 7| 6| 7| 1| 6| 4| 7| 9| 7| 8| 4| 6| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| A | 750 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________|____________| Penis | + | 1 | __________________________________________________________________________|____________| Preputial Gland | + + + M + + + + + + + + + + + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Prostate | + + + + + A + + + + + I + + + + + + + + + M + + +| 46 | __________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + A + + + + + + + + + + + + +| 47 | __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Interstitial Cell, Adenoma | | 1 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________|____________| Lymph Node | + + | 2 | __________________________________________________________________________|____________| Lymph Node, Bronchial | M + M + + + + M M M M + + M + M + + + M + M M M M| 28 | Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + I + + + + + + + + M + + + + + + + M + + M +| 43 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Lymph Node, Mediastinal | + + + + + + + + + + + + + + + M + + + + + + + + M| 40 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Thymus | + + + + + + + + M + M + + M + M + + + + + + M + +| 40 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| | __________________________________________________________________________|____________| Skin | + + + + + M + + + I + + + + + + + + + + + + + + +| 48 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Lymphoma Malignant | | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Larynx | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Alveolar/Bronchiolar Adenoma | X X X X X X X | 11 | Alveolar/Bronchiolar Adenoma, | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 56 NTP Experiment-Test: 05200-06 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC NITROMETHANE Date: 03/25/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 16:32:19 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 3| 7| 7| 5| 7| 7| 7| 7| 5| 7| 7| 7| 4| 5| 5| 7| 7| 6| 7| 6| 7| 7| | DAY ON TEST | 3| 3| 3| 9| 3| 0| 9| 2| 3| 3| 3| 9| 3| 3| 3| 9| 5| 8| 3| 3| 9| 3| 8| 3| 3| | | 4| 5| 5| 3| 4| 4| 5| 6| 7| 7| 4| 6| 6| 7| 6| 7| 1| 6| 4| 7| 9| 7| 8| 4| 6| | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| A | 750 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | __________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - cont | | | | | | Multiple | X | 1 | Alveolar/Bronchiolar Carcinoma | X X X X X X X | 10 | Alveolar/Bronchiolar Carcinoma, | | | Multiple | | 1 | Carcinoma, Metastatic, Harderian | | | Gland | X | 2 | Hepatocellular Carcinoma, Metastatic,| | | Liver | X | 2 | Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Carcinoma, Metastatic, Harderian | | | Gland | | 1 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | + | 5 | __________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Adenoma | X X X X X X X X X X X X X | 25 | Adenoma, Multiple | X X X X | 7 | Carcinoma | X X | 4 | Carcinoma, Multiple | | 1 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Lymphoma Malignant | | 1 | __________________________________________________________________________|____________| Ureter | + | 1 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Histiocytic Sarcoma | | 1 | Lymphoma Malignant | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 57 ------------------------------------------------------------ ---------- END OF REPORT ---------- ------------------------------------------------------------