TDMS Study 05203-09 Pathology Tables
NTP Experiment-Test: 05203-09 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC O-NITROTOLUENE Date: 11/16/00
Route: DOSED FEED Time: 09:50:01
MICE, FINAL#1
Facility: Southern Research Institute
Chemical CAS #: 88-72-2
Lock Date: 03/08/99
Cage Range: All
Reasons For Removal: All
Removal Date Range: All
Treatment Groups: Include All
a Number of animals examined microscopically at site and number of animals with lesion
Page 1
NTP Experiment-Test: 05203-09 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC O-NITROTOLUENE Date: 11/16/00
Route: DOSED FEED Time: 09:50:01
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 PPM 1250 PPM 2500 PPM 5000 PPM
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 60 60 60 60
Early Deaths
Moribund Sacrifice 4 6 7 22
Natural Death 4 8 6 33
Survivors
Terminal Sacrifice 52 46 47 5
Animals Examined Microscopically 60 60 60 60
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Gallbladder (54) (53) (54) (31)
Cyst 1 (2%) 1 (2%)
Intestine Large, Cecum (56) (53) (54) (32)
Edema 1 (2%)
Inflammation, Chronic 1 (3%)
Intestine Small, Jejunum (56) (53) (56) (34)
Peyer's Patch, Hyperplasia, Lymphoid 1 (2%) 1 (2%)
Peyer's Patch, Inflammation, Chronic, Focal,
Suppurative 1 (2%)
Liver (60) (59) (59) (60)
Angiectasis 1 (2%) 3 (5%)
Basophilic Focus 1 (2%) 6 (10%) 2 (3%) 6 (10%)
Clear Cell Focus 1 (2%) 2 (3%) 4 (7%)
Congestion, Focal 1 (2%) 2 (3%) 1 (2%)
Cyst 1 (2%)
Eosinophilic Focus 2 (3%) 3 (5%) 6 (10%) 28 (47%)
Hyperplasia, Focal, Lymphoid 2 (3%) 5 (8%) 2 (3%) 1 (2%)
Infiltration Cellular, Mixed Cell 45 (75%) 38 (64%) 37 (63%) 43 (72%)
Inflammation, Focal 1 (2%)
Mixed Cell Focus 1 (2%) 3 (5%)
Necrosis 3 (5%) 2 (3%) 13 (22%)
Tension Lipidosis 1 (2%) 1 (2%)
Bile Duct, Cyst 1 (2%)
Hepatocyte, Fatty Change 1 (2%)
Hepatocyte, Fatty Change, Diffuse 2 (3%)
Hepatocyte, Necrosis, Focal 6 (10%)
Hepatocyte, Tension Lipidosis 1 (2%)
Hepatocyte, Vacuolization Cytoplasmic,
Diffuse 2 (3%)
Hepatocyte, Vacuolization Cytoplasmic, Focal 1 (2%) 2 (3%) 2 (3%) 9 (15%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 2
NTP Experiment-Test: 05203-09 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC O-NITROTOLUENE Date: 11/16/00
Route: DOSED FEED Time: 09:50:01
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 PPM 1250 PPM 2500 PPM 5000 PPM
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Hepatocyte, Periportal, Depletion Glycogen 1 (2%)
Hepatocyte, Centrilobular, Fatty Change 2 (3%) 2 (3%)
Hepatocyte, Centrilobular, Necrosis 1 (2%)
Oval Cell, Hyperplasia 4 (7%)
Mesentery (11) (9) (9) (34)
Hemorrhage 1 (11%)
Inflammation, Chronic 1 (9%) 2 (6%)
Artery, Inflammation, Chronic 1 (11%)
Fat, Necrosis 6 (55%) 5 (56%) 4 (44%)
Pancreas (60) (57) (58) (57)
Acinus, Atrophy, Diffuse 2 (4%) 1 (2%) 1 (2%)
Acinus, Atrophy, Focal 1 (2%) 1 (2%)
Duct, Cyst 2 (3%) 1 (2%)
Duct, Inflammation, Chronic, Focal 2 (3%)
Stomach, Forestomach (60) (58) (58) (59)
Diverticulum 1 (2%)
Edema 1 (2%)
Inflammation, Focal 1 (2%) 2 (3%) 1 (2%)
Ulcer 1 (2%) 1 (2%) 1 (2%)
Epithelium, Hyperplasia 1 (2%) 4 (7%) 6 (10%) 1 (2%)
Stomach, Glandular (58) (57) (57) (52)
Erosion 1 (2%)
Inflammation, Focal 2 (4%)
Ulcer 1 (2%) 2 (4%) 1 (2%)
Glands, Degeneration, Cystic, Focal 2 (4%)
Tooth (8) (4) (2)
Peridontal Tissue, Inflammation, Chronic 8 (100%) 4 (100%) 2 (100%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Blood Vessel (2) (1) (1)
Aorta, Mineralization 1 (50%) 1 (100%)
Heart (60) (60) (59) (58)
Angiectasis 1 (2%)
Infiltration Cellular, Mixed Cell 1 (2%) 1 (2%)
Inflammation, Chronic, Focal 2 (3%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (59) (59) (58) (60)
Accessory Adrenal Cortical Nodule 2 (3%) 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 3
NTP Experiment-Test: 05203-09 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC O-NITROTOLUENE Date: 11/16/00
Route: DOSED FEED Time: 09:50:01
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 PPM 1250 PPM 2500 PPM 5000 PPM
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM - CONT
Cyst 2 (3%)
Cytoplasmic Alteration, Focal 1 (2%)
Hyperplasia, Focal 1 (2%)
Adrenal Medulla (59) (57) (58) (59)
Hyperplasia 2 (3%)
Islets, Pancreatic (60) (57) (60) (56)
Atrophy, Diffuse 1 (2%)
Parathyroid Gland (57) (55) (57) (50)
Cyst 1 (2%) 1 (2%) 1 (2%)
Pituitary Gland (58) (58) (53) (53)
Pars Distalis, Cyst 1 (2%)
Pars Distalis, Cytoplasmic Alteration, Focal 1 (2%) 4 (7%) 3 (6%)
Pars Distalis, Hyperplasia, Focal 1 (2%) 1 (2%) 2 (4%)
Thyroid Gland (60) (59) (58) (53)
Degeneration, Cystic, Focal 14 (23%) 16 (27%) 16 (28%) 13 (25%)
Hyperplasia, Lymphoid 1 (2%)
Inflammation, Chronic, Focal 2 (3%) 1 (2%)
Follicle, Cyst 1 (2%) 2 (4%)
Follicular Cell, Hyperplasia 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
Tissue NOS (1) (3) (3)
Abdominal, Cyst 1 (33%)
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Clitoral Gland (58) (57) (57) (58)
Degeneration, Cystic 11 (19%) 4 (7%) 5 (9%) 2 (3%)
Inflammation, Chronic 3 (5%) 2 (4%)
Pigmentation 3 (5%) 2 (4%) 1 (2%)
Ovary (59) (56) (56) (56)
Angiectasis 3 (5%) 2 (4%) 1 (2%)
Cyst 11 (19%) 16 (29%) 12 (21%) 11 (20%)
Cyst, Multiple 1 (2%) 2 (4%) 1 (2%)
Hemorrhage 1 (2%) 2 (4%) 1 (2%)
Hyperplasia, Tubular 1 (2%)
Mineralization 1 (2%)
Interstitial Cell, Hyperplasia 2 (4%)
Periovarian Tissue, Cyst 1 (2%)
Periovarian Tissue, Inflammation, Chronic 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 4
NTP Experiment-Test: 05203-09 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC O-NITROTOLUENE Date: 11/16/00
Route: DOSED FEED Time: 09:50:01
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 PPM 1250 PPM 2500 PPM 5000 PPM
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM - CONT
Uterus (60) (59) (60) (59)
Angiectasis 1 (2%) 2 (3%) 1 (2%)
Angiectasis, Focal 1 (2%)
Atrophy 1 (2%)
Congestion 1 (2%)
Cyst 1 (2%) 1 (2%) 3 (5%)
Hemorrhage 1 (2%) 1 (2%) 1 (2%)
Hydrometra 24 (40%) 24 (41%) 22 (37%) 15 (25%)
Hyperplasia, Focal, Histiocytic 1 (2%)
Inflammation, Suppurative 2 (3%) 1 (2%) 1 (2%) 2 (3%)
Pigmentation, Focal 1 (2%)
Endometrium, Hyperplasia, Cystic 54 (90%) 51 (86%) 53 (88%) 37 (63%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (60) (58) (60) (60)
Hemorrhage, Focal 1 (2%)
Hyperplasia 1 (2%) 2 (3%) 2 (3%)
Inflammation, Chronic, Focal 1 (2%)
Myelofibrosis 2 (3%)
Myelofibrosis, Focal 1 (2%)
Lymph Node (6) (3) (10) (11)
Iliac, Hematopoietic Cell Proliferation 1 (9%)
Iliac, Hemorrhage 1 (10%)
Iliac, Hyperplasia 1 (10%)
Iliac, Hyperplasia, Lymphoid 2 (20%)
Inguinal, Hyperplasia 1 (9%)
Mediastinal, Hematopoietic Cell Proliferation 1 (9%)
Mediastinal, Hemorrhage 3 (27%)
Mediastinal, Hyperplasia 1 (9%)
Mediastinal, Hyperplasia, Lymphoid 2 (18%)
Pancreatic, Hyperplasia, Lymphoid 1 (10%)
Renal, Hemorrhage 1 (10%)
Renal, Hyperplasia 2 (20%) 1 (9%)
Renal, Hyperplasia, Lymphoid 1 (10%)
Lymph Node, Mandibular (58) (55) (55) (48)
Hyperplasia 1 (2%)
Hyperplasia, Lymphoid 1 (2%) 1 (2%) 1 (2%)
Lymph Node, Mesenteric (53) (58) (57) (57)
Hemorrhage 1 (2%) 1 (2%)
Hyperplasia 2 (4%) 1 (2%) 2 (4%) 4 (7%)
Hyperplasia, Lymphoid 6 (11%) 2 (3%) 2 (4%) 2 (4%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 5
NTP Experiment-Test: 05203-09 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC O-NITROTOLUENE Date: 11/16/00
Route: DOSED FEED Time: 09:50:01
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 PPM 1250 PPM 2500 PPM 5000 PPM
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM - CONT
Hyperplasia, Mast Cell 1 (2%)
Spleen (59) (57) (58) (57)
Congestion 2 (3%)
Depletion Cellular 1 (2%)
Fibrosis 1 (2%)
Hematopoietic Cell Proliferation 11 (19%) 19 (33%) 21 (36%) 54 (95%)
Hyperplasia, Lymphoid 8 (14%) 5 (9%) 4 (7%) 2 (4%)
Pigmentation, Focal 1 (2%)
Thymus (57) (56) (55) (44)
Angiectasis 1 (2%) 1 (2%) 1 (2%)
Atrophy 1 (2%) 1 (2%)
Cyst 1 (2%)
Hyperplasia, Lymphoid 5 (9%) 2 (4%) 1 (2%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Mammary Gland (60) (59) (60) (59)
Ectasia 1 (2%) 1 (2%)
Skin (60) (60) (60) (60)
Subcutaneous Tissue, Angiectasis, Focal 1 (2%)
Subcutaneous Tissue, Cyst 1 (2%)
Subcutaneous Tissue, Edema 1 (2%) 2 (3%) 4 (7%)
Subcutaneous Tissue, Hemorrhage, Focal 1 (2%)
Subcutaneous Tissue, Inflammation, Chronic,
Focal 2 (3%) 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (60) (60) (60) (60)
Callus 1 (2%)
Fibrous Osteodystrophy 1 (2%)
Hyperostosis 2 (3%)
Femur, Callus 1 (2%)
Maxilla, Hyperostosis, Focal 1 (2%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (60) (60) (59) (58)
Atrophy, Focal 1 (2%) 1 (2%)
Compression, Focal 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 6
NTP Experiment-Test: 05203-09 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC O-NITROTOLUENE Date: 11/16/00
Route: DOSED FEED Time: 09:50:01
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 PPM 1250 PPM 2500 PPM 5000 PPM
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (60) (60) (59) (57)
Congestion 1 (2%) 2 (3%) 1 (2%) 1 (2%)
Edema, Focal 1 (2%)
Hemorrhage 2 (3%) 1 (2%) 1 (2%) 2 (4%)
Hyperplasia, Focal, Histiocytic 2 (3%)
Hyperplasia, Histiocytic 1 (2%)
Hyperplasia, Lymphoid 1 (2%) 1 (2%) 2 (3%) 1 (2%)
Infiltration Cellular, Mixed Cell 9 (16%)
Metaplasia, Osseous 1 (2%)
Alveolar Epithelium, Hyperplasia 1 (2%)
Artery, Mediastinum, Inflammation, Chronic 1 (2%)
Bronchus, Glands, Cyst 1 (2%)
Nose (60) (60) (59) (57)
Hemorrhage 1 (2%)
Mucosa, Glands, Dilatation, Focal 3 (5%) 1 (2%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
None
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (59) (56) (58) (59)
Atrophy, Focal 2 (3%)
Congestion 2 (3%) 3 (5%) 3 (5%) 1 (2%)
Cyst 2 (3%) 2 (3%) 1 (2%)
Hydronephrosis 1 (2%)
Hyperplasia, Lymphoid 4 (7%) 1 (2%)
Infiltration Cellular, Mixed Cell 1 (2%)
Inflammation, Suppurative 1 (2%)
Mineralization, Focal 1 (2%)
Nephropathy 9 (15%) 14 (25%) 10 (17%) 11 (19%)
Pigmentation 1 (2%)
Artery, Inflammation, Chronic 1 (2%)
Bilateral, Atrophy, Focal 1 (2%)
Bilateral, Fibrosis, Focal 1 (2%)
Bilateral, Inflammation, Chronic, Focal 1 (2%)
Pelvis, Dilatation 1 (2%) 1 (2%)
Pelvis, Hemorrhage 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 7
NTP Experiment-Test: 05203-09 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC O-NITROTOLUENE Date: 11/16/00
Route: DOSED FEED Time: 09:50:01
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 PPM 1250 PPM 2500 PPM 5000 PPM
____________________________________________________________________________________________________________________________________
URINARY SYSTEM - CONT
Renal Tubule, Accumulation, Hyaline Droplet 1 (2%) 3 (5%) 2 (3%) 10 (17%)
Renal Tubule, Casts Protein 6 (10%) 8 (14%) 7 (12%) 6 (10%)
Renal Tubule, Dilatation 1 (2%)
Renal Tubule, Necrosis 1 (2%)
Renal Tubule, Pigmentation 1 (2%) 3 (5%) 35 (59%)
Renal Tubule, Vacuolization Cytoplasmic 1 (2%)
Urinary Bladder (59) (54) (57) (54)
Transitional Epithelium, Hyperplasia, Focal 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 8
NTP Experiment-Test: 05203-09 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC O-NITROTOLUENE Date: 11/16/00
Route: DOSED FEED Time: 09:50:01
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 PPM 1250 PPM 2500 PPM 5000 PPM
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 60 60 60 60
Early Deaths
Natural Death 5 21 39 29
Moribund Sacrifice 3 5 21 31
Survivors
Terminal Sacrifice 52 34
Animals Examined Microscopically 60 60 60 60
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Gallbladder (54) (42) (31) (35)
Fibrosis 1 (2%)
Intestine Large, Cecum (56) (49) (36) (44)
Epithelium, Degeneration, Mucoid 1 (2%)
Intestine Small, Jejunum (56) (43) (35) (40)
Inflammation, Focal 1 (2%)
Ulcer 1 (2%)
Epithelium, Degeneration, Cystic, Focal 1 (2%)
Epithelium, Hyperplasia, Focal, Adenomatous 1 (2%)
Peyer's Patch, Hyperplasia, Lymphoid 1 (2%)
Peyer's Patch, Inflammation, Chronic 1 (2%)
Liver (60) (59) (57) (60)
Amyloid Deposition 1 (2%)
Angiectasis 1 (2%) 2 (4%) 1 (2%)
Basophilic Focus 6 (10%) 4 (7%)
Clear Cell Focus 3 (5%) 2 (3%)
Congestion, Focal 2 (3%) 1 (2%)
Eosinophilic Focus 3 (5%) 14 (24%) 1 (2%) 1 (2%)
Fibrosis, Focal 1 (2%) 1 (2%)
Infiltration Cellular, Mixed Cell 46 (77%) 40 (68%) 24 (42%) 29 (48%)
Inflammation, Chronic, Focal 1 (2%) 1 (2%)
Inflammation, Focal, Suppurative 1 (2%)
Mineralization, Focal 1 (2%)
Mixed Cell Focus 2 (3%)
Necrosis 1 (2%) 15 (25%) 27 (47%) 30 (50%)
Artery, Angiectasis 1 (2%)
Artery, Inflammation, Chronic 1 (2%)
Hepatocyte, Fatty Change, Diffuse 2 (3%)
Hepatocyte, Necrosis, Focal 2 (3%)
Hepatocyte, Syncytial Alteration, Focal 16 (27%) 26 (44%) 43 (75%) 39 (65%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 9
NTP Experiment-Test: 05203-09 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC O-NITROTOLUENE Date: 11/16/00
Route: DOSED FEED Time: 09:50:01
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 PPM 1250 PPM 2500 PPM 5000 PPM
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Hepatocyte, Tension Lipidosis 1 (2%)
Hepatocyte, Vacuolization Cytoplasmic,
Diffuse 10 (17%) 4 (7%)
Hepatocyte, Vacuolization Cytoplasmic, Focal 4 (7%) 5 (8%) 2 (4%) 1 (2%)
Hepatocyte, Periportal, Atrophy 1 (2%)
Hepatocyte, Centrilobular, Depletion Glycogen 1 (2%) 3 (5%)
Hepatocyte, Centrilobular, Fatty Change 1 (2%) 2 (3%) 5 (9%) 1 (2%)
Hepatocyte, Midzonal, Fatty Change 1 (2%)
Oval Cell, Hyperplasia 2 (3%)
Mesentery (12) (12) (42) (38)
Inflammation, Chronic 3 (25%) 2 (17%) 2 (5%)
Inflammation, Chronic, Focal 1 (2%)
Artery, Inflammation, Chronic 3 (25%) 1 (8%)
Fat, Necrosis 6 (50%) 1 (8%) 1 (2%)
Pancreas (59) (59) (59) (58)
Amyloid Deposition 1 (2%)
Acinus, Atrophy, Focal 1 (2%) 1 (2%) 1 (2%)
Duct, Cyst 1 (2%) 1 (2%)
Duct, Inflammation, Chronic, Focal 1 (2%)
Stomach, Forestomach (60) (60) (58) (60)
Inflammation, Focal 1 (2%) 3 (5%) 1 (2%)
Necrosis, Focal 1 (2%)
Ulcer 1 (2%) 3 (5%) 1 (2%)
Epithelium, Cyst 1 (2%)
Epithelium, Hyperplasia 2 (3%) 5 (8%) 6 (10%) 3 (5%)
Stomach, Glandular (57) (54) (53) (55)
Erosion 2 (4%)
Inflammation, Chronic, Focal 1 (2%)
Inflammation, Focal 1 (2%) 1 (2%)
Pigmentation, Focal 1 (2%)
Tooth (14) (9) (2)
Malformation 5 (36%)
Peridontal Tissue, Inflammation, Chronic 10 (71%) 9 (100%) 1 (50%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (60) (60) (60) (60)
Infiltration Cellular, Mixed Cell 1 (2%) 3 (5%) 1 (2%)
Inflammation, Chronic, Focal 1 (2%) 4 (7%) 1 (2%)
Mineralization 2 (3%) 1 (2%)
Thrombosis 1 (2%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 10
NTP Experiment-Test: 05203-09 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC O-NITROTOLUENE Date: 11/16/00
Route: DOSED FEED Time: 09:50:01
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 PPM 1250 PPM 2500 PPM 5000 PPM
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (60) (60) (58) (60)
Accessory Adrenal Cortical Nodule 1 (2%)
Cyst 2 (3%)
Cytoplasmic Alteration, Focal 7 (12%) 2 (3%)
Fibrosis 1 (2%)
Hypertrophy, Focal 1 (2%)
Inflammation, Chronic, Focal 1 (2%)
Parathyroid Gland (55) (54) (57) (56)
Amyloid Deposition 1 (2%)
Cyst 1 (2%)
Pituitary Gland (53) (50) (50) (46)
Pars Distalis, Cyst 2 (4%) 1 (2%)
Pars Distalis, Hyperplasia, Focal 1 (2%)
Rathke's Cleft, Cyst 1 (2%)
Thyroid Gland (59) (59) (59) (59)
Degeneration, Cystic, Focal 10 (17%) 11 (19%) 3 (5%)
Follicular Cell, Hyperplasia 1 (2%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
Tissue NOS (2) (2) (3)
Pelvic, Inflammation, Chronic, Focal 1 (33%)
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Coagulating Gland (2) (1)
Inflammation, Chronic 1 (50%) 1 (100%)
Epididymis (60) (60) (60) (60)
Inflammation, Chronic 1 (2%)
Preputial Gland (60) (59) (59) (60)
Degeneration, Cystic 28 (47%) 27 (46%) 16 (27%) 1 (2%)
Inflammation, Chronic 1 (2%) 5 (8%) 1 (2%)
Prostate (60) (59) (57) (58)
Inflammation, Chronic 1 (2%) 7 (12%) 4 (7%)
Seminal Vesicle (60) (60) (60) (60)
Congestion 1 (2%) 1 (2%)
Dilatation 2 (3%) 1 (2%) 1 (2%)
Inflammation, Chronic 1 (2%)
Inflammation, Suppurative 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 11
NTP Experiment-Test: 05203-09 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC O-NITROTOLUENE Date: 11/16/00
Route: DOSED FEED Time: 09:50:01
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 PPM 1250 PPM 2500 PPM 5000 PPM
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (60) (60) (60) (60)
Depletion Cellular 1 (2%)
Hemorrhage, Focal 2 (3%) 1 (2%)
Hyperplasia 3 (5%) 2 (3%)
Thrombosis 1 (2%)
Erythroid Cell, Depletion Cellular 1 (2%)
Lymph Node (3) (4) (7) (1)
Iliac, Hematopoietic Cell Proliferation 1 (14%)
Iliac, Hyperplasia 1 (14%)
Mediastinal, Hemorrhage 1 (33%) 1 (25%) 3 (43%)
Mediastinal, Hyperplasia 1 (25%) 1 (14%)
Mediastinal, Pigmentation 1 (25%)
Pancreatic, Hemorrhage 1 (25%)
Lymph Node, Mandibular (59) (55) (50) (54)
Hyperplasia, Lymphoid 1 (2%)
Lymph Node, Mesenteric (60) (55) (56) (53)
Hematopoietic Cell Proliferation 1 (2%)
Hemorrhage 1 (2%) 2 (4%)
Hyperplasia 1 (2%)
Hyperplasia, Lymphoid 1 (2%)
Spleen (60) (60) (58) (60)
Amyloid Deposition 1 (2%)
Depletion Cellular 1 (2%) 7 (12%) 2 (3%)
Hematopoietic Cell Proliferation 13 (22%) 24 (40%) 49 (84%) 60 (100%)
Hyperplasia, Lymphoid 2 (3%) 3 (5%)
Pigmentation, Focal 1 (2%)
Thymus (57) (52) (45) (54)
Atrophy 3 (6%) 3 (7%) 6 (11%)
Cyst 4 (7%) 3 (6%) 1 (2%)
Hyperplasia, Lymphoid 1 (2%)
Epithelial Cell, Hyperplasia 1 (2%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Skin (60) (60) (60) (60)
Ulcer, Chronic, Focal 1 (2%)
Artery, Subcutaneous Tissue, Inflammation,
Chronic 2 (3%)
Prepuce, Subcutaneous Tissue, Inflammation,
Suppurative 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 12
NTP Experiment-Test: 05203-09 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC O-NITROTOLUENE Date: 11/16/00
Route: DOSED FEED Time: 09:50:01
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 PPM 1250 PPM 2500 PPM 5000 PPM
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM - CONT
Subcutaneous Tissue, Edema 3 (5%) 14 (23%) 22 (37%)
Subcutaneous Tissue, Hemorrhage, Focal 1 (2%) 2 (3%)
Subcutaneous Tissue, Inflammation, Chronic,
Focal 1 (2%) 1 (2%) 2 (3%)
Subcutaneous Tissue, Necrosis 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (60) (60) (60) (60)
Fracture 1 (2%)
Skeletal Muscle (1) (6) (35) (47)
Hemorrhage, Focal 2 (6%) 2 (4%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
None
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (60) (60) (60) (60)
Congestion 1 (2%) 1 (2%) 1 (2%)
Cyst 1 (2%)
Hemorrhage 1 (2%) 1 (2%) 5 (8%)
Hyperplasia, Histiocytic 2 (3%) 1 (2%)
Infiltration Cellular, Polymorphonuclear 1 (2%)
Infiltration Cellular, Mixed Cell 4 (7%) 9 (15%) 3 (5%)
Inflammation, Chronic, Focal 3 (5%)
Alveolar Epithelium, Hyperplasia 1 (2%) 1 (2%) 1 (2%) 1 (2%)
Bronchus, Glands, Cyst 1 (2%)
Nose (60) (60) (60) (60)
Mucosa, Glands, Dilatation, Focal 1 (2%)
Nasolacrimal Duct, Inflammation 1 (2%)
Sinus, Foreign Body 1 (2%)
Sinus, Inflammation, Chronic, Suppurative 1 (2%)
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SPECIAL SENSES SYSTEM
Eye (1) (1)
Atrophy 1 (100%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 13
NTP Experiment-Test: 05203-09 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC O-NITROTOLUENE Date: 11/16/00
Route: DOSED FEED Time: 09:50:01
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B6C3F1 MICE MALE 0 PPM 1250 PPM 2500 PPM 5000 PPM
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SPECIAL SENSES SYSTEM - CONT
Inflammation, Chronic 1 (100%)
Cornea, Necrosis, Focal 1 (100%)
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URINARY SYSTEM
Kidney (58) (59) (58) (60)
Amyloid Deposition 1 (2%)
Atrophy 1 (2%) 1 (2%)
Atrophy, Focal 2 (3%)
Congestion 5 (9%) 3 (5%)
Cyst 2 (3%) 4 (7%) 1 (2%) 1 (2%)
Fibrosis, Focal 2 (3%)
Hyperplasia, Lymphoid 1 (2%) 3 (5%)
Infarct 1 (2%)
Infiltration Cellular, Mixed Cell 1 (2%)
Inflammation, Chronic 1 (2%) 1 (2%)
Inflammation, Chronic, Focal 1 (2%)
Inflammation, Focal, Suppurative 1 (2%)
Inflammation, Suppurative 1 (2%) 3 (5%)
Metaplasia, Focal, Osseous 1 (2%)
Nephropathy 56 (97%) 53 (90%) 34 (59%) 18 (30%)
Artery, Inflammation, Chronic 2 (3%)
Papilla, Necrosis 1 (2%)
Pelvis, Dilatation 2 (3%)
Renal Tubule, Accumulation, Hyaline Droplet 1 (2%) 2 (3%) 5 (9%) 3 (5%)
Renal Tubule, Dilatation 1 (2%) 4 (7%)
Renal Tubule, Necrosis 1 (2%) 2 (3%)
Renal Tubule, Pigmentation 1 (2%) 6 (10%) 32 (55%) 35 (58%)
Renal Tubule, Vacuolization Cytoplasmic 1 (2%) 1 (2%)
Urethra (1) (2)
Inflammation, Focal 1 (100%) 2 (100%)
Urinary Bladder (59) (59) (59) (60)
Calculus Micro Observation Only 1 (2%)
Inflammation, Chronic 1 (2%)
Transitional Epithelium, Hyperplasia 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 14
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