TDMS Study 05205-08 Pathology Tables
NTP Experiment-Test: 05205-08 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC P-NITROTOLUENE Date: 02/08/00
Route: DOSED FEED Time: 09:26:17
FINAL#1 CHRONIC STUDY FOR MICE
Facility: Southern Research Institute
Chemical CAS #: 99-99-0
Lock Date: 08/24/98
Cage Range: All
Reasons For Removal: All
Removal Date Range: All
Treatment Groups: Include All
a Number of animals examined microscopically at site and number of animals with lesion
Page 1
NTP Experiment-Test: 05205-08 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC P-NITROTOLUENE Date: 02/08/00
Route: DOSED FEED Time: 09:26:17
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 1250 2500 5000
PPM PPM PPM PPM
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially in Study 50 50 50 50
Early Deaths
Moribund Sacrifice 2 1 5 1
Natural Death 2 2 2
Survivors
Terminal Sacrifice 46 47 41 49
Natural Death 2
Animals Examined Microscopically 50 50 50 50
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Intestine Small, Duodenum (49) (49) (49) (50)
Adenocarcinoma 1 (2%)
Intestine Small, Jejunum (49) (48) (48) (50)
Carcinoma 1 (2%)
Liver (49) (50) (50) (50)
Hepatocellular Carcinoma 3 (6%) 4 (8%) 1 (2%)
Hepatocellular Adenoma 6 (12%) 3 (6%) 2 (4%) 4 (8%)
Hepatocellular Adenoma, Multiple 1 (2%)
Histiocytic Sarcoma 1 (2%)
Sarcoma, Metastatic, Skeletal Muscle 1 (2%)
Mesentery (3) (3) (4) (7)
Histiocytic Sarcoma 1 (25%)
Sarcoma, Metastatic, Skeletal Muscle 1 (25%)
Pancreas (49) (50) (49) (50)
Histiocytic Sarcoma 1 (2%)
Sarcoma, Metastatic, Skeletal Muscle 1 (2%)
Salivary Glands (50) (50) (50) (50)
Stomach, Forestomach (49) (50) (50) (50)
Squamous Cell Papilloma 3 (6%) 2 (4%) 1 (2%)
Tongue (1)
Squamous Cell Carcinoma 1 (100%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
None
____________________________________________________________________________________________________________________________________
NTP Experiment-Test: 05205-08 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC P-NITROTOLUENE Date: 02/08/00
Route: DOSED FEED Time: 09:26:17
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 1250 2500 5000
PPM PPM PPM PPM
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (50) (50) (50) (50)
Capsule, Sarcoma, Metastatic, Skeletal Muscle 1 (2%)
Adrenal Medulla (50) (50) (50) (50)
Pheochromocytoma Benign 1 (2%)
Pituitary Gland (45) (49) (49) (45)
Pars Distalis, Adenoma 1 (2%) 1 (2%)
Pars Intermedia, Carcinoma 1 (2%)
Thyroid Gland (50) (50) (49) (50)
Follicular Cell, Adenoma 1 (2%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Ovary (48) (50) (50) (49)
Cystadenoma 3 (6%) 1 (2%) 2 (4%)
Granulosa Cell Tumor Malignant 1 (2%)
Granulosa Cell Tumor Benign 1 (2%)
Hemangioma 1 (2%)
Luteoma 1 (2%)
Sarcoma, Metastatic, Skeletal Muscle 1 (2%)
Uterus (50) (50) (50) (50)
Leiomyoma 1 (2%) 1 (2%)
Leiomyosarcoma 1 (2%)
Cervix, Hemangiosarcoma 1 (2%)
Endometrium, Adenoma 1 (2%)
Endometrium, Polyp Stromal 3 (6%) 1 (2%) 4 (8%) 1 (2%)
Endometrium, Sarcoma Stromal 1 (2%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (50) (50) (50) (50)
Histiocytic Sarcoma 1 (2%)
Lymph Node (2) (5) (13) (4)
Fibrosarcoma, Metastatic, Skin 2 (15%)
Bronchial, Histiocytic Sarcoma 1 (8%)
Iliac, Histiocytic Sarcoma 1 (8%)
Iliac, Sarcoma, Metastatic, Skeletal Muscle 1 (8%)
Inguinal, Fibrosarcoma, Metastatic, Skin 1 (8%)
Inguinal, Histiocytic Sarcoma 1 (8%)
Mediastinal, Sarcoma, Metastatic, Skeletal
NTP Experiment-Test: 05205-08 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC P-NITROTOLUENE Date: 02/08/00
Route: DOSED FEED Time: 09:26:17
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 1250 2500 5000
PPM PPM PPM PPM
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM - cont
Muscle 1 (8%)
Pancreatic, Histiocytic Sarcoma 1 (8%)
Renal, Histiocytic Sarcoma 1 (8%)
Lymph Node, Mandibular (50) (48) (48) (47)
Histiocytic Sarcoma 1 (2%)
Lymph Node, Mesenteric (48) (49) (49) (50)
Histiocytic Sarcoma 1 (2%)
Spleen (49) (50) (49) (50)
Hemangiosarcoma 1 (2%)
Histiocytic Sarcoma 1 (2%)
Thymus (48) (49) (47) (46)
Histiocytic Sarcoma 1 (2%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Mammary Gland (50) (50) (50) (50)
Carcinoma 1 (2%)
Skin (50) (50) (50) (50)
Squamous Cell Carcinoma 1 (2%)
Subcutaneous Tissue, Fibrosarcoma 1 (2%) 4 (8%)
Subcutaneous Tissue, Hemangiosarcoma 1 (2%)
Subcutaneous Tissue, Lipoma 1 (2%)
Subcutaneous Tissue, Sarcoma 1 (2%)
Subcutaneous Tissue, Sarcoma NOS, Metastatic,
Skin 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Skeletal Muscle (1) (3) (1)
Sarcoma NOS, Metastatic, Skeletal Muscle 2 (67%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (50) (50) (50) (50)
Carcinoma, Metastatic, Pituitary Gland 1 (2%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (50) (50) (50) (50)
Alveolar/Bronchiolar Adenoma 5 (10%) 2 (4%) 2 (4%) 5 (10%)
Alveolar/Bronchiolar Carcinoma 1 (2%) 2 (4%) 3 (6%)
Fibrosarcoma, Metastatic, Skin 2 (4%)
Hepatocellular Carcinoma, Metastatic, Liver 1 (2%)
NTP Experiment-Test: 05205-08 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC P-NITROTOLUENE Date: 02/08/00
Route: DOSED FEED Time: 09:26:17
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 1250 2500 5000
PPM PPM PPM PPM
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM - cont
Histiocytic Sarcoma 1 (2%)
Sarcoma, Metastatic, Skeletal Muscle 1 (2%)
Serosa, Sarcoma, Metastatic, Skeletal Muscle 1 (2%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Ear (1)
External Ear, Melanoma Malignant 1 (100%)
Harderian Gland (2) (3) (2) (3)
Adenoma 2 (100%) 2 (67%) 1 (50%) 3 (100%)
Carcinoma 1 (33%) 1 (50%)
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (49) (50) (50) (50)
____________________________________________________________________________________________________________________________________
SYSTEMIC LESIONS
Multiple Organs *(50) *(50) *(50) *(50)
Histiocytic Sarcoma 1 (2%)
Lymphoma Malignant 3 (6%) 5 (10%) 9 (18%) 5 (10%)
____________________________________________________________________________________________________________________________________
* Number of animals with any tissue examined microscopically
Page 5
NTP Experiment-Test: 05205-08 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC P-NITROTOLUENE Date: 02/08/00
Route: DOSED FEED Time: 09:26:17
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 1250 2500 5000
PPM PPM PPM PPM
____________________________________________________________________________________________________________________________________
TUMOR SUMMARY
Total Animals with Primary Neoplasms (b) 21 23 29 25
Total Primary Neoplasms 32 28 41 27
Total Animals with Benign Neoplasms 17 14 14 14
Total Benign Neoplasms 22 15 18 15
Total Animals with Malignant Neoplasms 9 13 21 12
Total Malignant Neoplasms 10 13 23 12
Total Animals with Metastatic Neoplasms 7 1
Total Metastatic Neoplasm 18 1
Total Animals with Malignant Neoplasms
Uncertain Primary Site
Total Animals with Neoplasms Uncertain-
Benign or Malignant
Total Uncertain Neoplasms
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
b Primary tumors: all tumors except metastatic tumors
NTP Experiment-Test: 05205-08 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC P-NITROTOLUENE Date: 02/08/00
Route: DOSED FEED Time: 09:26:17
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 1250 2500 5000
PPM PPM PPM PPM
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially in Study 50 50 50 50
Early Deaths
Moribund Sacrifice 2 2 3 6
Natural Death 2 2 2 2
Survivors
Terminal Sacrifice 46 46 45 42
Animals Examined Microscopically 50 50 50 50
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Intestine Large, Cecum (48) (48) (48) (49)
Carcinoma 1 (2%)
Intestine Small, Duodenum (48) (49) (49) (49)
Polyp Adenomatous 2 (4%)
Intestine Small, Jejunum (48) (49) (48) (49)
Carcinoma 1 (2%)
Polyp Adenomatous 1 (2%)
Intestine Small, Ileum (48) (49) (48) (49)
Liver (50) (50) (50) (50)
Cholangiocarcinoma 1 (2%)
Hemangiosarcoma 1 (2%) 1 (2%)
Hepatoblastoma 1 (2%)
Hepatocellular Carcinoma 8 (16%) 11 (22%) 10 (20%) 12 (24%)
Hepatocellular Carcinoma, Multiple 1 (2%) 1 (2%)
Hepatocellular Adenoma 13 (26%) 9 (18%) 12 (24%) 11 (22%)
Hepatocellular Adenoma, Multiple 1 (2%) 4 (8%) 3 (6%) 4 (8%)
Hepatocholangiocarcinoma 2 (4%) 1 (2%)
Histiocytic Sarcoma 1 (2%) 1 (2%) 1 (2%)
Ito Cell Tumor Malignant 1 (2%)
Mesentery (6) (7) (8) (5)
Cholangiocarcinoma, Metastatic, Liver 1 (14%)
Hemangiosarcoma 1 (13%) 1 (20%)
Hepatocholangiocarcinoma, Metastatic, Liver 1 (17%) 1 (13%)
Histiocytic Sarcoma 1 (17%)
Pancreas (50) (50) (50) (50)
Cholangiocarcinoma, Metastatic, Liver 1 (2%)
Salivary Glands (50) (50) (50) (50)
Stomach, Forestomach (50) (50) (50) (50)
Squamous Cell Papilloma 1 (2%) 2 (4%) 2 (4%) 3 (6%)
Tongue (1)
Squamous Cell Carcinoma 1 (100%)
____________________________________________________________________________________________________________________________________
NTP Experiment-Test: 05205-08 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC P-NITROTOLUENE Date: 02/08/00
Route: DOSED FEED Time: 09:26:17
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 1250 2500 5000
PPM PPM PPM PPM
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (50) (50) (50) (50)
Histiocytic Sarcoma 1 (2%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (49) (50) (49) (50)
Adenoma 2 (4%) 1 (2%) 1 (2%)
Capsule, Adenoma 2 (4%) 3 (6%) 3 (6%)
Subcapsular, Adenoma 1 (2%)
Adrenal Medulla (49) (50) (49) (49)
Pheochromocytoma Benign 1 (2%)
Islets, Pancreatic (50) (50) (50) (50)
Adenoma 1 (2%)
Pituitary Gland (46) (49) (49) (45)
Pars Intermedia, Adenoma 1 (2%)
Thyroid Gland (50) (50) (49) (49)
Follicular Cell, Adenoma 2 (4%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Epididymis (50) (50) (49) (50)
Histiocytic Sarcoma 1 (2%)
Prostate (50) (50) (49) (50)
Testes (50) (50) (50) (50)
Interstitial Cell, Adenoma 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (50) (50) (50) (50)
Hemangioma 1 (2%)
Lymph Node (5) (5) (3) (5)
Renal, Hepatocholangiocarcinoma, Metastatic,
Liver 1 (20%)
Lymph Node, Mandibular (48) (50) (45) (48)
Lymph Node, Mesenteric (50) (49) (49) (49)
Hepatocholangiocarcinoma, Metastatic, Liver 1 (2%)
Histiocytic Sarcoma 1 (2%)
Spleen (50) (50) (50) (50)
NTP Experiment-Test: 05205-08 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC P-NITROTOLUENE Date: 02/08/00
Route: DOSED FEED Time: 09:26:17
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 1250 2500 5000
PPM PPM PPM PPM
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM - cont
Hemangioma 1 (2%)
Hemangiosarcoma 1 (2%) 1 (2%) 1 (2%)
Thymus (47) (40) (44) (45)
Histiocytic Sarcoma 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Skin (50) (50) (50) (50)
Squamous Cell Papilloma 1 (2%)
Subcutaneous Tissue, Fibrosarcoma 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Skeletal Muscle (1) (1) (1)
Cholangiocarcinoma, Metastatic, Liver 1 (100%)
Hepatocholangiocarcinoma, Metastatic, Liver 1 (100%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
None
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (50) (50) (50) (50)
Alveolar/Bronchiolar Adenoma 5 (10%) 8 (16%) 6 (12%) 11 (22%)
Alveolar/Bronchiolar Adenoma, Multiple 1 (2%) 1 (2%) 2 (4%) 2 (4%)
Alveolar/Bronchiolar Carcinoma 1 (2%) 5 (10%) 4 (8%) 4 (8%)
Alveolar/Bronchiolar Carcinoma, Multiple 1 (2%) 1 (2%) 2 (4%)
Cholangiocarcinoma, Metastatic, Liver 1 (2%)
Hepatocellular Carcinoma, Metastatic, Liver 1 (2%) 3 (6%) 1 (2%)
Hepatocholangiocarcinoma, Metastatic, Liver 1 (2%)
Histiocytic Sarcoma 1 (2%)
Mediastinum, Fibrosarcoma, Metastatic, Skin 1 (2%)
Mediastinum, Hepatocholangiocarcinoma,
Metastatic, Liver 1 (2%)
Mediastinum, Histiocytic Sarcoma 1 (2%)
____________________________________________________________________________________________________________________________________
NTP Experiment-Test: 05205-08 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC P-NITROTOLUENE Date: 02/08/00
Route: DOSED FEED Time: 09:26:17
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 1250 2500 5000
PPM PPM PPM PPM
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Harderian Gland (3) (4) (6) (2)
Adenoma 3 (100%) 3 (75%) 6 (100%) 1 (50%)
Carcinoma 1 (25%) 1 (50%)
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (50) (49) (50) (50)
Renal Tubule, Adenoma 1 (2%)
____________________________________________________________________________________________________________________________________
SYSTEMIC LESIONS
Multiple Organs *(50) *(50) *(50) *(50)
Histiocytic Sarcoma 1 (2%) 1 (2%) 2 (4%)
Lymphoma Malignant 3 (6%) 3 (6%) 1 (2%) 4 (8%)
____________________________________________________________________________________________________________________________________
* Number of animals with any tissue examined microscopically
Page 10
NTP Experiment-Test: 05205-08 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC P-NITROTOLUENE Date: 02/08/00
Route: DOSED FEED Time: 09:26:17
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 1250 2500 5000
PPM PPM PPM PPM
____________________________________________________________________________________________________________________________________
TUMOR SUMMARY
Total Animals with Primary Neoplasms (b) 31 38 41 44
Total Primary Neoplasms 47 64 58 67
Total Animals with Benign Neoplasms 25 28 30 33
Total Benign Neoplasms 29 38 35 39
Total Animals with Malignant Neoplasms 14 24 20 21
Total Malignant Neoplasms 18 26 23 28
Total Animals with Metastatic Neoplasms 2 5 2
Total Metastatic Neoplasm 3 8 6
Total Animals with Malignant Neoplasms
Uncertain Primary Site
Total Animals with Neoplasms Uncertain-
Benign or Malignant
Total Uncertain Neoplasms
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
b Primary tumors: all tumors except metastatic tumors
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