TDMS Study 50215-03 Pathology Tables
NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 MICE FINAL#1 Facility: Battelle Columbus Laboratory Chemical CAS #: 107-13-1 Lock Date: 11/03/99 Cage Range: All Reasons For Removal: All Removal Date Range: All Treatment Groups: Include All Page 1 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 5| 7| 7| 7| 7| 3| 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| DAY ON TEST | 2| 0| 6| 3| 2| 2| 2| 3| 3| 2| 3| 3| 3| 5| 3| 2| 3| 2| 3| 3| 2| 5| 3| 3| 0| | 9| 8| 2| 0| 9| 9| 9| 8| 1| 9| 8| 0| 0| 9| 0| 9| 0| 9| 0| 1| 9| 3| 1| 0| 8| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 2 | Basophilic Focus | X | Clear Cell Focus | X X X X X| Eosinophilic Focus | X X X | Hematopoietic Cell Proliferation | 2 | Inflammation, Chronic Active | 1 1 1 1 1 1 1 1 1 1 2 1 1 1 1 1 1 1 1 1 | Mixed Cell Focus | X X X| Necrosis | 2 | Pigmentation | 1 2 1 | Vacuolization Cytoplasmic | 1 1 1 2 2 2 | Centrilobular, Degeneration | 2 | Portal, Infiltration Cellular, | | Lymphocyte | | |__________________________________________________________________________| Mesentery | + + + + + | Fibrosis | | Fat, Inflammation, Chronic Active | | Fat, Necrosis | 4 4 | |__________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 2 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 5| 7| 7| 7| 7| 3| 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| DAY ON TEST | 2| 0| 6| 3| 2| 2| 2| 3| 3| 2| 3| 3| 3| 5| 3| 2| 3| 2| 3| 3| 2| 5| 3| 3| 0| | 9| 8| 2| 0| 9| 9| 9| 8| 1| 9| 8| 0| 0| 9| 0| 9| 0| 9| 0| 1| 9| 3| 1| 0| 8| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | Fibrosis | | Infiltration Cellular, Mononuclear | | Cell | 1 | Acinus, Atrophy | 2 2| |__________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperkeratosis, Diffuse | 2 | Hyperkeratosis, Focal | | Inflammation, Chronic Active, | | Multifocal | 2 | Ulcer, Multifocal | 2 | Epithelium, Hyperplasia, Focal | 2 | |__________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | 4 | Inflammation, Chronic Active | 3 | Mineralization | 1 1 | Glands, Ectasia | 1 1 1 1 1 | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| Aorta, Mineralization | | |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Mineralization | 2 | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Accessory Adrenal Cortical Nodule | | Hematopoietic Cell Proliferation | 2 | Hyperplasia | 2 | Subcapsular, Hyperplasia | 2 2 2 2 2 2 2 1 2 2 2 2 2 2 2 2 2 2 2 1 2 2 2 2 2| __________________________________________|__________________________________________________________________________| Page 3 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 5| 7| 7| 7| 7| 3| 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| DAY ON TEST | 2| 0| 6| 3| 2| 2| 2| 3| 3| 2| 3| 3| 3| 5| 3| 2| 3| 2| 3| 3| 2| 5| 3| 3| 0| | 9| 8| 2| 0| 9| 9| 9| 8| 1| 9| 8| 0| 0| 9| 0| 9| 0| 9| 0| 1| 9| 3| 1| 0| 8| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ENDOCRINE SYSTEM - cont | | |__________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 | |__________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Parathyroid Gland | M M M + + + + + + + + + + + + + + + + + + + + + +| Cyst | | |__________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Pars Distalis, Hyperplasia | 2 1 2 | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicle, Cyst | 2 2 2 1 2 | Follicular Cell, Hyperplasia | 1 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 1 | Inflammation, Chronic Active | 4 2 | |__________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 3 | Atrophy | 3 3 | Cyst | 2 3 2 3 2 1 1 4 2| |__________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 3| Inflammation, Suppurative | | Endometrium, Hyperplasia, Cystic | 2 4 1 1 2 4 2 4 4 2 2 4 2 2 4 4 4 4 | Lymphatic, Angiectasis | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | __________________________________________|__________________________________________________________________________| Page 4 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 5| 7| 7| 7| 7| 3| 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| DAY ON TEST | 2| 0| 6| 3| 2| 2| 2| 3| 3| 2| 3| 3| 3| 5| 3| 2| 3| 2| 3| 3| 2| 5| 3| 3| 0| | 9| 8| 2| 0| 9| 9| 9| 8| 1| 9| 8| 0| 0| 9| 0| 9| 0| 9| 0| 1| 9| 3| 1| 0| 8| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Myelofibrosis | | Myeloid Cell, Hyperplasia | 4 | |__________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + +| Mediastinal, Hyperplasia, Lymphoid | 2 | |__________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | 3 3 | |__________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Ectasia | | Hematopoietic Cell Proliferation | | Hyperplasia, Lymphoid | 4 | Inflammation, Chronic Active | 2 | |__________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 2 2 2 4 2 2 2 2 | Hyperplasia, Lymphoid | 1 1 1 2 1 1 3 3 1 3| |__________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | 3 2 4 3 3 | Ectopic Parathyroid Gland | 1 | Hyperplasia, Lymphoid | 4 | Thymocyte, Necrosis | 3 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Fibrosis | 3 | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 5 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 5| 7| 7| 7| 7| 3| 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| DAY ON TEST | 2| 0| 6| 3| 2| 2| 2| 3| 3| 2| 3| 3| 3| 5| 3| 2| 3| 2| 3| 3| 2| 5| 3| 3| 0| | 9| 8| 2| 0| 9| 9| 9| 8| 1| 9| 8| 0| 0| 9| 0| 9| 0| 9| 0| 1| 9| 3| 1| 0| 8| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| MUSCULOSKELETAL SYSTEM - cont | | Skeletal Muscle | + + + | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Erythrophagocytosis | | Inflammation, Chronic Active | | Mineralization | | Alveolar Epithelium, Hyperplasia | 3 | Alveolus, Infiltration Cellular, | | Histiocyte | 4 | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Nasolacrimal Duct, Inflammation, | | Suppurative | | |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + M + + + + + + + + + + +| Hyperplasia | 3 4 3 2 | Infiltration Cellular, Mononuclear | | Cell | 1 1 1 1 1 1 1 1 1 2 1 1 1 1 2 1 1 | |__________________________________________________________________________| Zymbal's Gland | + | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Infarct | 3 | Inflammation, Chronic Active | 1 | Metaplasia, Osseous | | Mineralization | | __________________________________________|__________________________________________________________________________| Page 6 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 5| 7| 7| 7| 7| 3| 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| DAY ON TEST | 2| 0| 6| 3| 2| 2| 2| 3| 3| 2| 3| 3| 3| 5| 3| 2| 3| 2| 3| 3| 2| 5| 3| 3| 0| | 9| 8| 2| 0| 9| 9| 9| 8| 1| 9| 8| 0| 0| 9| 0| 9| 0| 9| 0| 1| 9| 3| 1| 0| 8| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| URINARY SYSTEM - cont | | Nephropathy | 1 1 1 2 1 1 1 | Medulla, Cyst | 2 | Renal Tubule, Vacuolization | | Cytoplasmic | 4 | |__________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________ Page 7 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| | DAY ON TEST | 3| 3| 2| 3| 3| 3| 3| 2| 2| 2| 3| 3| 3| 3| 0| 2| 3| 2| 2| 3| 0| 3| 3| 7| 7| | | 1| 0| 9| 0| 1| 0| 1| 9| 9| 9| 0| 1| 0| 1| 4| 9| 0| 9| 9| 0| 1| 0| 1| 3| 7| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | | 1 2.0| Basophilic Focus | | 1 | Clear Cell Focus | | 5 | Eosinophilic Focus | X X X X X X | 9 | Hematopoietic Cell Proliferation | | 1 2.0| Inflammation, Chronic Active | 1 1 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 | 42 1.0| Mixed Cell Focus | | 3 | Necrosis | | 1 2.0| Pigmentation | 1 1 1 | 6 1.2| Vacuolization Cytoplasmic | 1 1 1 | 9 1.3| Centrilobular, Degeneration | | 1 2.0| Portal, Infiltration Cellular, | | | Lymphocyte | 2 2 | 2 2.0| |__________________________________________________________________________|____________| Mesentery | + + + + | 9 | Fibrosis | 3 | 1 3.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 8 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| | DAY ON TEST | 3| 3| 2| 3| 3| 3| 3| 2| 2| 2| 3| 3| 3| 3| 0| 2| 3| 2| 2| 3| 0| 3| 3| 7| 7| | | 1| 0| 9| 0| 1| 0| 1| 9| 9| 9| 0| 1| 0| 1| 4| 9| 0| 9| 9| 0| 1| 0| 1| 3| 7| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | Fat, Inflammation, Chronic Active | 3 | 1 3.0| Fat, Necrosis | 4 4 4 | 5 4.0| |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Fibrosis | 2 | 1 2.0| Infiltration Cellular, Mononuclear | | | Cell | 1 | 2 1.0| Acinus, Atrophy | | 2 2.0| |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 | 36 1.0| |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperkeratosis, Diffuse | | 1 2.0| Hyperkeratosis, Focal | 1 | 1 1.0| Inflammation, Chronic Active, | | | Multifocal | | 1 2.0| Ulcer, Multifocal | | 1 2.0| Epithelium, Hyperplasia, Focal | 1 | 2 1.5| |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia, Focal | | 1 4.0| Inflammation, Chronic Active | | 1 3.0| Mineralization | | 2 1.0| Glands, Ectasia | 1 1 1 1 1 1 | 11 1.0| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Aorta, Mineralization | 2 | 1 2.0| |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Mineralization | | 1 2.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 9 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| | DAY ON TEST | 3| 3| 2| 3| 3| 3| 3| 2| 2| 2| 3| 3| 3| 3| 0| 2| 3| 2| 2| 3| 0| 3| 3| 7| 7| | | 1| 0| 9| 0| 1| 0| 1| 9| 9| 9| 0| 1| 0| 1| 4| 9| 0| 9| 9| 0| 1| 0| 1| 3| 7| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Accessory Adrenal Cortical Nodule | 1 | 1 1.0| Hematopoietic Cell Proliferation | | 1 2.0| Hyperplasia | | 1 2.0| Subcapsular, Hyperplasia | 2 2 2 2 2 2 2 2 2 2 2 2 3 3 2 2 2 2 2 2 1 2 2 2 1| 50 2.0| |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | | 1 2.0| |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Parathyroid Gland | + + + + M + + M + + + + + M + + + + + M + + + + +| 43 | Cyst | 2 | 1 2.0| |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + I + + + M + + + + + + +| 48 | Pars Distalis, Hyperplasia | 1 2 2 2 2 1 2| 10 1.7| |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Follicle, Cyst | 2 | 6 1.8| Follicular Cell, Hyperplasia | | 1 1.0| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | 1 | 2 1.0| Inflammation, Chronic Active | 2 | 3 2.7| |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | | 1 3.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 10 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| | DAY ON TEST | 3| 3| 2| 3| 3| 3| 3| 2| 2| 2| 3| 3| 3| 3| 0| 2| 3| 2| 2| 3| 0| 3| 3| 7| 7| | | 1| 0| 9| 0| 1| 0| 1| 9| 9| 9| 0| 1| 0| 1| 4| 9| 0| 9| 9| 0| 1| 0| 1| 3| 7| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | Atrophy | 3 3 2 4| 6 3.0| Cyst | 4 2 2 | 12 2.3| |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | | 1 3.0| Inflammation, Suppurative | 2 | 1 2.0| Endometrium, Hyperplasia, Cystic | 4 4 4 4 4 2 1 4 2 4 4 4 4 2 4 3 4 4 3 4 2 4| 40 3.2| Lymphatic, Angiectasis | 4 | 1 4.0| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Myelofibrosis | 2 | 1 2.0| Myeloid Cell, Hyperplasia | | 1 4.0| |__________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + + + + | 37 | Mediastinal, Hyperplasia, Lymphoid | 4 | 2 3.0| |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + M + + + + + + + + + + + + +| 49 | Hyperplasia, Lymphoid | 2 2 | 4 2.5| |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Ectasia | 4 | 1 4.0| Hematopoietic Cell Proliferation | 2 | 1 2.0| Hyperplasia, Lymphoid | 3 2 | 3 3.0| Inflammation, Chronic Active | | 1 2.0| |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hematopoietic Cell Proliferation | 2 2 2 2 3 2 2 2 2 | 17 2.2| Hyperplasia, Lymphoid | 3 2 1 2 2 1 2 3 | 18 1.8| |__________________________________________________________________________|____________| Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Atrophy | 4| 6 3.2| Ectopic Parathyroid Gland | | 1 1.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 11 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| | DAY ON TEST | 3| 3| 2| 3| 3| 3| 3| 2| 2| 2| 3| 3| 3| 3| 0| 2| 3| 2| 2| 3| 0| 3| 3| 7| 7| | | 1| 0| 9| 0| 1| 0| 1| 9| 9| 9| 0| 1| 0| 1| 4| 9| 0| 9| 9| 0| 1| 0| 1| 3| 7| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | Hyperplasia, Lymphoid | | 1 4.0| Thymocyte, Necrosis | | 1 3.0| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Subcutaneous Tissue, Fibrosis | | 1 3.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Skeletal Muscle | | 3 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Erythrophagocytosis | 3| 1 3.0| Inflammation, Chronic Active | 1 | 1 1.0| Mineralization | 2 | 1 2.0| Alveolar Epithelium, Hyperplasia | 4 | 2 3.5| Alveolus, Infiltration Cellular, | | | Histiocyte | 3| 2 3.5| |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Nasolacrimal Duct, Inflammation, | | | Suppurative | 2 | 1 2.0| |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 12 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| | DAY ON TEST | 3| 3| 2| 3| 3| 3| 3| 2| 2| 2| 3| 3| 3| 3| 0| 2| 3| 2| 2| 3| 0| 3| 3| 7| 7| | | 1| 0| 9| 0| 1| 0| 1| 9| 9| 9| 0| 1| 0| 1| 4| 9| 0| 9| 9| 0| 1| 0| 1| 3| 7| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| RESPIRATORY SYSTEM - cont | | | Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Hyperplasia | 3 | 5 3.0| Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 1 1| 40 1.1| |__________________________________________________________________________|____________| Zymbal's Gland | | 1 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infarct | 2 | 2 2.5| Inflammation, Chronic Active | 1 | 2 1.0| Metaplasia, Osseous | 2 2 | 2 2.0| Mineralization | 1 1 1 1 | 4 1.0| Nephropathy | 1 1 1 2 1 1 1 | 14 1.1| Medulla, Cyst | | 1 2.0| Renal Tubule, Vacuolization | | | Cytoplasmic | | 1 4.0| |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 13 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 4| 6| 7| 7| 7| 7| 7| 3| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 6| 7| 7| 7| 7| 7| 7| DAY ON TEST | 2| 5| 3| 2| 3| 2| 2| 5| 2| 2| 2| 2| 3| 3| 3| 3| 6| 3| 4| 3| 3| 2| 3| 3| 2| | 4| 3| 1| 9| 1| 9| 9| 4| 9| 1| 9| 9| 0| 0| 0| 0| 8| 0| 0| 1| 0| 9| 0| 1| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Suppurative | 3 | Peyer's Patch, Hyperplasia, Lymphoid | 3 | |__________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 3 2 | Basophilic Focus | X X| Clear Cell Focus | X | Eosinophilic Focus | X X X X | Hematopoietic Cell Proliferation | 1 1| Hyperplasia | | Inflammation, Chronic Active | 3 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 | Mineralization | | Mixed Cell Focus | X X X X X X X X X | Necrosis | | Pigmentation | 1 1 1 1 1 1 1 1 | Vacuolization Cytoplasmic | 2 1 1 1 1 1 2 1 | |__________________________________________________________________________| Mesentery | + + + + + + | Fat, Inflammation, Chronic Active | | Fat, Necrosis | 4 4 4 4 4 | |__________________________________________________________________________| Oral Mucosa | | __________________________________________|__________________________________________________________________________| Page 14 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 4| 6| 7| 7| 7| 7| 7| 3| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 6| 7| 7| 7| 7| 7| 7| DAY ON TEST | 2| 5| 3| 2| 3| 2| 2| 5| 2| 2| 2| 2| 3| 3| 3| 3| 6| 3| 4| 3| 3| 2| 3| 3| 2| | 4| 3| 1| 9| 1| 9| 9| 4| 9| 1| 9| 9| 0| 0| 0| 0| 8| 0| 0| 1| 0| 9| 0| 1| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | |__________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 2 | Inflammation, Chronic Active | | |__________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperkeratosis, Focal | 2 | Inflammation, Chronic Active, Focal | 2 | Inflammation, Chronic Active, | | Multifocal | | Ulcer, Multifocal | | Epithelium, Hyperplasia, Focal | 4 | Epithelium, Hyperplasia, Multifocal | | |__________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Glands, Ectasia | 1 1 1 1| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| Aorta, Mineralization | | |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | | Mineralization | | Valve, Inflammation, Suppurative | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Cyst | 2 | Hematopoietic Cell Proliferation | 2 | Hyperplasia | | __________________________________________|__________________________________________________________________________| Page 15 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 4| 6| 7| 7| 7| 7| 7| 3| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 6| 7| 7| 7| 7| 7| 7| DAY ON TEST | 2| 5| 3| 2| 3| 2| 2| 5| 2| 2| 2| 2| 3| 3| 3| 3| 6| 3| 4| 3| 3| 2| 3| 3| 2| | 4| 3| 1| 9| 1| 9| 9| 4| 9| 1| 9| 9| 0| 0| 0| 0| 8| 0| 0| 1| 0| 9| 0| 1| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ENDOCRINE SYSTEM - cont | | Metaplasia, Osseous | 2 | Subcapsular, Hyperplasia | 1 2 2 2 2 2 2 1 2 1 2 2 1 1 2 1 1 2 2 2 2 2 2 2 2| |__________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + M M + + + + + + M + M + + + M| Cyst | | |__________________________________________________________________________| Pituitary Gland | M + + + + + + + + + + + + + + + + + + + + + + + +| Pars Distalis, Angiectasis | 3 | Pars Distalis, Hyperplasia | 2 2 2 1 | Pars Distalis, Pigmentation | 2 | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Lymphocyte | 2 | Follicle, Cyst | 2 2 2 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Clitoral Gland | + M + + + + + + + M + + + + + + + + + + + + + + +| Inflammation, Chronic Active | | Pigmentation | 2 | |__________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Atrophy | 4 4 4 4| Cyst | 2 2 2 2 3 2 2 2 2 | Pigmentation | | |__________________________________________________________________________| Oviduct | | |__________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 2 3 | __________________________________________|__________________________________________________________________________| Page 16 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 4| 6| 7| 7| 7| 7| 7| 3| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 6| 7| 7| 7| 7| 7| 7| DAY ON TEST | 2| 5| 3| 2| 3| 2| 2| 5| 2| 2| 2| 2| 3| 3| 3| 3| 6| 3| 4| 3| 3| 2| 3| 3| 2| | 4| 3| 1| 9| 1| 9| 9| 4| 9| 1| 9| 9| 0| 0| 0| 0| 8| 0| 0| 1| 0| 9| 0| 1| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| GENITAL SYSTEM - cont | | Cyst | | Inflammation, Suppurative | 2 | Endometrium, Hyperplasia, Cystic | 4 4 2 4 3 4 4 2 4 4 3 2 3 3 4 3 3 2 | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Myelofibrosis | | Myeloid Cell, Hyperplasia | 4| |__________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + | Mediastinal, Hematopoietic Cell | | Proliferation | | Mediastinal, Hyperplasia, Lymphoid | 1 | Renal, Hematopoietic Cell | | Proliferation | | Renal, Hyperplasia, Lymphoid | | |__________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + M| Hematopoietic Cell Proliferation | | Hyperplasia, Lymphoid | 2 | |__________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Hematopoietic Cell Proliferation | | Hyperplasia, Lymphoid | | Hyperplasia, Plasma Cell | 4 | Infiltration Cellular, | | Polymorphonuclear | 3 | Inflammation, Chronic Active | | |__________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 2 2 2 2 2 2 2 4 2 4 3| Hyperplasia, Lymphoid | 2 2 2 2 1 2 1 1 3| |__________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | 3 | Ectopic Parathyroid Gland | 2 | __________________________________________|__________________________________________________________________________| Page 17 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 4| 6| 7| 7| 7| 7| 7| 3| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 6| 7| 7| 7| 7| 7| 7| DAY ON TEST | 2| 5| 3| 2| 3| 2| 2| 5| 2| 2| 2| 2| 3| 3| 3| 3| 6| 3| 4| 3| 3| 2| 3| 3| 2| | 4| 3| 1| 9| 1| 9| 9| 4| 9| 1| 9| 9| 0| 0| 0| 0| 8| 0| 0| 1| 0| 9| 0| 1| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 | |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | | Ulcer | | Epidermis, Hyperplasia | | Subcutaneous Tissue, Fibrosis | 3 3 | Subcutaneous Tissue, Hemorrhage | 3 | Subcutaneous Tissue, Infiltration | | Cellular, Mast Cell | | Subcutaneous Tissue, Inflammation, | | Chronic Active | 2 2 | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skeletal Muscle | + | Inflammation, Chronic Active | | Arteriole, Mineralization | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Larynx | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | 2 | Inflammation, Suppurative | | Mineralization | | Thrombosis | 3 | Alveolar Epithelium, Hyperplasia | 2 1 | Alveolus, Infiltration Cellular, | | __________________________________________|__________________________________________________________________________| Page 18 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 4| 6| 7| 7| 7| 7| 7| 3| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 6| 7| 7| 7| 7| 7| 7| DAY ON TEST | 2| 5| 3| 2| 3| 2| 2| 5| 2| 2| 2| 2| 3| 3| 3| 3| 6| 3| 4| 3| 3| 2| 3| 3| 2| | 4| 3| 1| 9| 1| 9| 9| 4| 9| 1| 9| 9| 0| 0| 0| 0| 8| 0| 0| 1| 0| 9| 0| 1| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| RESPIRATORY SYSTEM - cont | | Histiocyte | | Mediastinum, Inflammation, Chronic | | Active | 1 | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | | Atrophy | | Cornea, Inflammation, Chronic Active | | |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 | Infiltration Cellular, Mononuclear | | Cell | 1 2 1 1 2 1 1 1 1 1 1 1 1 1 1 1 | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Infarct | 2 | Inflammation, Chronic Active | | Metaplasia, Osseous | | Mineralization | 1 | Nephropathy | 1 1 1 | Cortex, Cyst | | Renal Tubule, Hyperplasia | | |__________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + M + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________ Page 19 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 6| 6| 6| 7| 7| 7| 6| 6| 7| 7| 6| 7| 6| 7| 6| 7| 6| 7| 7| 7| 6| 7| 7| 5| | DAY ON TEST | 2| 4| 9| 9| 3| 3| 3| 3| 7| 3| 2| 8| 2| 4| 2| 6| 2| 8| 2| 2| 3| 4| 2| 3| 6| | | 9| 8| 6| 4| 0| 0| 0| 3| 3| 0| 9| 8| 9| 7| 9| 5| 9| 3| 9| 9| 0| 3| 9| 0| 3| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Suppurative | | 1 3.0| Peyer's Patch, Hyperplasia, Lymphoid | | 1 3.0| |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | 2 | 3 2.3| Basophilic Focus | X X X X | 6 | Clear Cell Focus | X X | 3 | Eosinophilic Focus | X X X | 7 | Hematopoietic Cell Proliferation | 2 1 | 4 1.3| Hyperplasia | 2| 1 2.0| Inflammation, Chronic Active | 1 1 1 4 1 1 1 1 1 1 1 1 1 1 1 | 34 1.2| Mineralization | 2 | 1 2.0| Mixed Cell Focus | X X X X X X | 15 | Necrosis | 2 3 2 | 3 2.3| Pigmentation | 1 1 1 1 1 1 2 1 1 1 | 18 1.1| Vacuolization Cytoplasmic | 1 2 1 1 | 12 1.3| |__________________________________________________________________________|____________| Mesentery | + + + + | 10 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 20 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 6| 6| 6| 7| 7| 7| 6| 6| 7| 7| 6| 7| 6| 7| 6| 7| 6| 7| 7| 7| 6| 7| 7| 5| | DAY ON TEST | 2| 4| 9| 9| 3| 3| 3| 3| 7| 3| 2| 8| 2| 4| 2| 6| 2| 8| 2| 2| 3| 4| 2| 3| 6| | | 9| 8| 6| 4| 0| 0| 0| 3| 3| 0| 9| 8| 9| 7| 9| 5| 9| 3| 9| 9| 0| 3| 9| 0| 3| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | Fat, Inflammation, Chronic Active | 4 | 1 4.0| Fat, Necrosis | 4 4 | 7 4.0| |__________________________________________________________________________|____________| Oral Mucosa | + | 1 | |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | | 1 2.0| Inflammation, Chronic Active | 4 | 1 4.0| |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 1 1 1 1 1 2 1 1 1 2 1 2 2 | 32 1.1| |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperkeratosis, Focal | | 1 2.0| Inflammation, Chronic Active, Focal | | 1 2.0| Inflammation, Chronic Active, | | | Multifocal | 2 | 1 2.0| Ulcer, Multifocal | 2 | 1 2.0| Epithelium, Hyperplasia, Focal | | 1 4.0| Epithelium, Hyperplasia, Multifocal | 2 | 1 2.0| |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Glands, Ectasia | 1 1 1 1 1 1 1 | 11 1.0| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Aorta, Mineralization | 3 | 1 3.0| |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Chronic Active | 1 | 1 1.0| Mineralization | 1 | 1 1.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 21 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 6| 6| 6| 7| 7| 7| 6| 6| 7| 7| 6| 7| 6| 7| 6| 7| 6| 7| 7| 7| 6| 7| 7| 5| | DAY ON TEST | 2| 4| 9| 9| 3| 3| 3| 3| 7| 3| 2| 8| 2| 4| 2| 6| 2| 8| 2| 2| 3| 4| 2| 3| 6| | | 9| 8| 6| 4| 0| 0| 0| 3| 3| 0| 9| 8| 9| 7| 9| 5| 9| 3| 9| 9| 0| 3| 9| 0| 3| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| CARDIOVASCULAR SYSTEM - cont | | | Valve, Inflammation, Suppurative | 4 | 1 4.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Cyst | | 1 2.0| Hematopoietic Cell Proliferation | 1 | 2 1.5| Hyperplasia | 2 | 1 2.0| Metaplasia, Osseous | | 1 2.0| Subcapsular, Hyperplasia | 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 2 1 2 2 2 2 2 1 2| 49 1.8| |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Parathyroid Gland | + + M M + + + M M + + + + + + M + + + + + + + + M| 39 | Cyst | 2 | 1 2.0| |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Pars Distalis, Angiectasis | | 1 3.0| Pars Distalis, Hyperplasia | 2 2 2 2 | 8 1.9| Pars Distalis, Pigmentation | | 1 2.0| |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Lymphocyte | | 1 2.0| Follicle, Cyst | 2 | 4 2.0| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | Inflammation, Chronic Active | 2 | 1 2.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 22 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 6| 6| 6| 7| 7| 7| 6| 6| 7| 7| 6| 7| 6| 7| 6| 7| 6| 7| 7| 7| 6| 7| 7| 5| | DAY ON TEST | 2| 4| 9| 9| 3| 3| 3| 3| 7| 3| 2| 8| 2| 4| 2| 6| 2| 8| 2| 2| 3| 4| 2| 3| 6| | | 9| 8| 6| 4| 0| 0| 0| 3| 3| 0| 9| 8| 9| 7| 9| 5| 9| 3| 9| 9| 0| 3| 9| 0| 3| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | Pigmentation | | 1 2.0| |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | 4 | 1 4.0| Atrophy | 4 4 4 3 | 8 3.9| Cyst | 2 2 2 2 2 2 2 4 2 4 2 | 20 2.3| Pigmentation | 2 | 1 2.0| |__________________________________________________________________________|____________| Oviduct | +| 1 | |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | | 2 2.5| Cyst | 3 | 1 3.0| Inflammation, Suppurative | 3 | 2 2.5| Endometrium, Hyperplasia, Cystic | 4 2 2 4 1 4 4 3 4 4 3 3 4 2 2 2 4 2 4 4 4 3 4| 41 3.2| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Myelofibrosis | 2 | 1 2.0| Myeloid Cell, Hyperplasia | 4 4 4 4 | 5 4.0| |__________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + +| 32 | Mediastinal, Hematopoietic Cell | | | Proliferation | 3 | 1 3.0| Mediastinal, Hyperplasia, Lymphoid | 3 4 1 | 4 2.3| Renal, Hematopoietic Cell | | | Proliferation | 3 | 1 3.0| Renal, Hyperplasia, Lymphoid | 4 | 1 4.0| |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Hematopoietic Cell Proliferation | 2 | 1 2.0| Hyperplasia, Lymphoid | 2 4 2 3 2 2 | 7 2.4| |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 23 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 6| 6| 6| 7| 7| 7| 6| 6| 7| 7| 6| 7| 6| 7| 6| 7| 6| 7| 7| 7| 6| 7| 7| 5| | DAY ON TEST | 2| 4| 9| 9| 3| 3| 3| 3| 7| 3| 2| 8| 2| 4| 2| 6| 2| 8| 2| 2| 3| 4| 2| 3| 6| | | 9| 8| 6| 4| 0| 0| 0| 3| 3| 0| 9| 8| 9| 7| 9| 5| 9| 3| 9| 9| 0| 3| 9| 0| 3| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | Lymph Node, Mesenteric | + + + M + + + + + + + + + + + + + + + + + + + + +| 49 | Angiectasis | 4 | 1 4.0| Hematopoietic Cell Proliferation | 3 | 1 3.0| Hyperplasia, Lymphoid | 3 | 1 3.0| Hyperplasia, Plasma Cell | | 1 4.0| Infiltration Cellular, | | | Polymorphonuclear | | 1 3.0| Inflammation, Chronic Active | 4 3 | 2 3.5| |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hematopoietic Cell Proliferation | 2 3 2 3 3 3 4 3 2 2 4 3| 23 2.7| Hyperplasia, Lymphoid | 2 3 1 1 3 2 2 | 16 1.9| |__________________________________________________________________________|____________| Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Atrophy | 4 4 4 4 4 4 3 3 4 4 | 11 3.7| Ectopic Parathyroid Gland | 1| 2 1.5| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | | 1 2.0| |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Chronic Active | 3 | 1 3.0| Ulcer | 4 | 1 4.0| Epidermis, Hyperplasia | 3 | 1 3.0| Subcutaneous Tissue, Fibrosis | | 2 3.0| Subcutaneous Tissue, Hemorrhage | | 1 3.0| Subcutaneous Tissue, Infiltration | | | Cellular, Mast Cell | 2 | 1 2.0| Subcutaneous Tissue, Inflammation, | | | Chronic Active | | 2 2.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 24 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 6| 6| 6| 7| 7| 7| 6| 6| 7| 7| 6| 7| 6| 7| 6| 7| 6| 7| 7| 7| 6| 7| 7| 5| | DAY ON TEST | 2| 4| 9| 9| 3| 3| 3| 3| 7| 3| 2| 8| 2| 4| 2| 6| 2| 8| 2| 2| 3| 4| 2| 3| 6| | | 9| 8| 6| 4| 0| 0| 0| 3| 3| 0| 9| 8| 9| 7| 9| 5| 9| 3| 9| 9| 0| 3| 9| 0| 3| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| MUSCULOSKELETAL SYSTEM - cont | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Skeletal Muscle | + + | 3 | Inflammation, Chronic Active | 2 | 1 2.0| Arteriole, Mineralization | 4 | 1 4.0| _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Larynx | + | 1 | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Chronic Active | 2 1 | 3 1.7| Inflammation, Suppurative | 4 4 | 2 4.0| Mineralization | 2 | 1 2.0| Thrombosis | 4 | 2 3.5| Alveolar Epithelium, Hyperplasia | | 2 1.5| Alveolus, Infiltration Cellular, | | | Histiocyte | 3 2 2 | 3 2.3| Mediastinum, Inflammation, Chronic | | | Active | | 1 1.0| |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + | 1 | Atrophy | 4 | 1 4.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 25 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 6| 6| 6| 7| 7| 7| 6| 6| 7| 7| 6| 7| 6| 7| 6| 7| 6| 7| 7| 7| 6| 7| 7| 5| | DAY ON TEST | 2| 4| 9| 9| 3| 3| 3| 3| 7| 3| 2| 8| 2| 4| 2| 6| 2| 8| 2| 2| 3| 4| 2| 3| 6| | | 9| 8| 6| 4| 0| 0| 0| 3| 3| 0| 9| 8| 9| 7| 9| 5| 9| 3| 9| 9| 0| 3| 9| 0| 3| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| SPECIAL SENSES SYSTEM - cont | | | Cornea, Inflammation, Chronic Active | 2 | 1 2.0| |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | 4 3 4 | 4 3.3| Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 1 1 1 1 2 1 2 1 1 1 | 30 1.1| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infarct | 2 4 | 3 2.7| Inflammation, Chronic Active | 3 2 | 2 2.5| Metaplasia, Osseous | 2 | 1 2.0| Mineralization | 1 2 | 3 1.3| Nephropathy | 1 1 1 | 6 1.0| Cortex, Cyst | 4 1 | 2 2.5| Renal Tubule, Hyperplasia | 2 | 1 2.0| |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 26 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 1| 5| 6| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 4| 3| 3| 3| 2| 3| 2| 2| 3| 3| 3| 3| 1| 3| 9| 1| 3| 3| 2| 3| 3| 3| 3| 2| | 0| 6| 0| 1| 1| 9| 0| 9| 1| 0| 0| 0| 0| 5| 0| 0| 6| 9| 1| 9| 0| 1| 0| 1| 9| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 10 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + M + + + + + + + + + + +| |__________________________________________________________________________| Gallbladder | + + + + + + M + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + M + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + M + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| Peyer's Patch, Hyperplasia, Lymphoid | | |__________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 2 2 2 2 2 | Basophilic Focus | | Clear Cell Focus | X X | Eosinophilic Focus | X X X X X X X X X X | Hematopoietic Cell Proliferation | 1 | Infarct | | Inflammation, Chronic Active | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| Mixed Cell Focus | X X X X X X X X X | Necrosis | 1 | Pigmentation | 1 2 2 1 1 2 | Vacuolization Cytoplasmic | 2 1 1 1 1 1 1 1| |__________________________________________________________________________| Mesentery | + + + + + + + | Fat, Necrosis | 4 4 4 4 4 4 4 | |__________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 2 1 1 1 1 | |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 27 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 1| 5| 6| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 4| 3| 3| 3| 2| 3| 2| 2| 3| 3| 3| 3| 1| 3| 9| 1| 3| 3| 2| 3| 3| 3| 3| 2| | 0| 6| 0| 1| 1| 9| 0| 9| 1| 0| 0| 0| 0| 5| 0| 0| 6| 9| 1| 9| 0| 1| 0| 1| 9| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 10 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | Salivary Glands | + + + + + + + + + + + + + M + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 1 1 1 1 1 1 1 1 1 1 2 1 1 1 1| |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Erosion, Focal | | Hyperkeratosis, Diffuse | 2 2 | Inflammation, Chronic Active, Focal | 1 3 | Epithelium, Hyperplasia, Focal | 2 3 | |__________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | | Mineralization | | Glands, Ectasia | 1 1 1 | |__________________________________________________________________________| Tooth | | Malformation | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | | Thrombosis | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | | Subcapsular, Hyperplasia | 2 2 3 2 2 2 2 2 2 2 2 2 2 2 1 2 2 2 2 1 1 2 2| |__________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Parathyroid Gland | + + + + + M + M M M + + + M + M + + + M + + + + M| |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 28 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 1| 5| 6| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 4| 3| 3| 3| 2| 3| 2| 2| 3| 3| 3| 3| 1| 3| 9| 1| 3| 3| 2| 3| 3| 3| 3| 2| | 0| 6| 0| 1| 1| 9| 0| 9| 1| 0| 0| 0| 0| 5| 0| 0| 6| 9| 1| 9| 0| 1| 0| 1| 9| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 10 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ENDOCRINE SYSTEM - cont | | Pituitary Gland | + + + + + + + + + + + + + M + + + + + + + + + + +| Pars Distalis, Angiectasis | | Pars Distalis, Cyst | | Pars Distalis, Hyperplasia | 2 2 2 | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + M + + + + + + + + + + +| Follicle, Cyst | 2 2 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Clitoral Gland | + + + + + + + + + + + + + M + + + + + + + + + + +| Duct, Cyst | | |__________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Atrophy | 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4| Cyst | 2 2 2 2 2 3 2 1 2 2 2 2 4 2 4| Mineralization | | Pigmentation | | |__________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 3 | Endometrium, Hyperplasia, Cystic | 1 1 4 4 1 4 1 3 2 1 2 1 4 1 2| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Myeloid Cell, Hyperplasia | 4 4 | |__________________________________________________________________________| Lymph Node | + + + + + + + + + + + +| Inguinal, Hyperplasia, Lymphoid | 2 | Mediastinal, Hyperplasia, Lymphoid | | |__________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + M + M + + M + + + + + + + + + + +| Hyperplasia, Lymphoid | 2 2 4 2 3 | __________________________________________|__________________________________________________________________________| Page 29 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 1| 5| 6| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 4| 3| 3| 3| 2| 3| 2| 2| 3| 3| 3| 3| 1| 3| 9| 1| 3| 3| 2| 3| 3| 3| 3| 2| | 0| 6| 0| 1| 1| 9| 0| 9| 1| 0| 0| 0| 0| 5| 0| 0| 6| 9| 1| 9| 0| 1| 0| 1| 9| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 10 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | |__________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Ectasia | 2 | Hematopoietic Cell Proliferation | 4 | Hyperplasia, Lymphoid | | Infiltration Cellular, | | Polymorphonuclear | | |__________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 2 2 2 2 2 2 2 4 2 4 2 3 | Hyperplasia, Lymphoid | 1 3 1 2 3 1 1 3 3 1 1| |__________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + + + + + + + + M +| Atrophy | 4 4 3| Hyperplasia, Lymphoid | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Fibrosis | | Subcutaneous Tissue, Inflammation, | | Chronic Active | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skeletal Muscle | + | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 30 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 1| 5| 6| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 4| 3| 3| 3| 2| 3| 2| 2| 3| 3| 3| 3| 1| 3| 9| 1| 3| 3| 2| 3| 3| 3| 3| 2| | 0| 6| 0| 1| 1| 9| 0| 9| 1| 0| 0| 0| 0| 5| 0| 0| 6| 9| 1| 9| 0| 1| 0| 1| 9| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 10 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| RESPIRATORY SYSTEM - cont | | Hemorrhage | | Inflammation, Chronic Active | 1 | Necrosis | | Pigmentation | 2 | Alveolar Epithelium, Hyperplasia | 1 3 | Alveolus, Infiltration Cellular, | | Histiocyte | 2 2 | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + M + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | + + | Atrophy | 4 | Synechia | 4 | Retina, Degeneration | 1 | |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + M + + + + + + + + + + +| Hyperplasia | 2 2 3 | Infiltration Cellular, Mononuclear | | Cell | 2 1 1 1 2 1 1 1 1 2 2 2 1 1 2 1 1| _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Mineralization | | Nephropathy | 1 1 1 2 1 1 | Cortex, Cyst | 2 | Cortex, Hydronephrosis | | |__________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________ Page 31 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 2| 3| 1| 2| 3| 2| 3| 2| 2| 3| 4| 2| 2| 1| 0| 3| 3| 2| 9| 3| 3| 2| 3| 3| | | 1| 9| 0| 6| 9| 0| 9| 0| 9| 9| 1| 5| 9| 9| 1| 8| 0| 0| 9| 5| 0| 1| 9| 0| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A | 10 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | |__________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Peyer's Patch, Hyperplasia, Lymphoid | 4 | 1 4.0| |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | 2 2 2 4 | 9 2.2| Basophilic Focus | X X X X | 4 | Clear Cell Focus | X X X X | 6 | Eosinophilic Focus | X X X X X X X X | 18 | Hematopoietic Cell Proliferation | 3 1 | 3 1.7| Infarct | 4 | 1 4.0| Inflammation, Chronic Active | 1 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 40 1.0| Mixed Cell Focus | X X X X X X X | 16 | Necrosis | 1 1 | 3 1.0| Pigmentation | 1 1 1 1 1 1 1 | 13 1.2| Vacuolization Cytoplasmic | 2 1 1 2 2 1 1 | 15 1.3| |__________________________________________________________________________|____________| Mesentery | + + + | 10 | Fat, Necrosis | 4 | 8 4.0| |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 32 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 2| 3| 1| 2| 3| 2| 3| 2| 2| 3| 4| 2| 2| 1| 0| 3| 3| 2| 9| 3| 3| 2| 3| 3| | | 1| 9| 0| 6| 9| 0| 9| 0| 9| 9| 1| 5| 9| 9| 1| 8| 0| 0| 9| 5| 0| 1| 9| 0| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A | 10 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | 1 2 1 1 | 9 1.2| |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Infiltration Cellular, Mononuclear | | | Cell | 1 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 32 1.1| |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Erosion, Focal | 2 | 1 2.0| Hyperkeratosis, Diffuse | | 2 2.0| Inflammation, Chronic Active, Focal | | 2 2.0| Epithelium, Hyperplasia, Focal | 2 1 1 | 5 1.8| |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia, Focal | 2 | 1 2.0| Mineralization | 1 | 1 1.0| Glands, Ectasia | 1 1 1 | 6 1.0| |__________________________________________________________________________|____________| Tooth | + | 1 | Malformation | X | 1 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Chronic Active | 1 | 1 1.0| Thrombosis | 3 | 1 3.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | 2| 1 2.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 33 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 2| 3| 1| 2| 3| 2| 3| 2| 2| 3| 4| 2| 2| 1| 0| 3| 3| 2| 9| 3| 3| 2| 3| 3| | | 1| 9| 0| 6| 9| 0| 9| 0| 9| 9| 1| 5| 9| 9| 1| 8| 0| 0| 9| 5| 0| 1| 9| 0| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A | 10 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ENDOCRINE SYSTEM - cont | | | Subcapsular, Hyperplasia | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 1 2 2 2| 47 1.9| |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Parathyroid Gland | M + M + M + + + M + + + + M + + + + + + + + + + +| 37 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + I| 48 | Pars Distalis, Angiectasis | 3 | 1 3.0| Pars Distalis, Cyst | 2 | 1 2.0| Pars Distalis, Hyperplasia | 2 2 2 | 6 2.0| |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Follicle, Cyst | 2 | 3 2.0| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Duct, Cyst | 2| 1 2.0| |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | 4 4 | 2 4.0| Atrophy | 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4| 45 4.0| Cyst | 2 2 2 2 2 2 2 2 2 2 2 2| 27 2.1| Mineralization | 1 1 | 2 1.0| Pigmentation | 1 1 2 | 3 1.3| |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | 3 2| 3 2.7| Endometrium, Hyperplasia, Cystic | 1 1 2 3 2 1 1 3 1 4 4 3 3 2| 29 2.2| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 34 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 2| 3| 1| 2| 3| 2| 3| 2| 2| 3| 4| 2| 2| 1| 0| 3| 3| 2| 9| 3| 3| 2| 3| 3| | | 1| 9| 0| 6| 9| 0| 9| 0| 9| 9| 1| 5| 9| 9| 1| 8| 0| 0| 9| 5| 0| 1| 9| 0| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A | 10 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Myeloid Cell, Hyperplasia | 4 | 3 4.0| |__________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + + + | 30 | Inguinal, Hyperplasia, Lymphoid | | 1 2.0| Mediastinal, Hyperplasia, Lymphoid | 3 3 2 4 3 | 5 3.0| |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| 47 | Hyperplasia, Lymphoid | 2 1 2 2 4 2 2 | 12 2.3| |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Ectasia | 2 | 2 2.0| Hematopoietic Cell Proliferation | | 1 4.0| Hyperplasia, Lymphoid | 4 3 4 | 3 3.7| Infiltration Cellular, | | | Polymorphonuclear | 2 | 1 2.0| |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hematopoietic Cell Proliferation | 3 2 3 3 2 2 2 2 3 3 2 4 | 24 2.5| Hyperplasia, Lymphoid | 3 2 2 1 3 2 | 17 1.9| |__________________________________________________________________________|____________| Thymus | + + + + + + + + + + + M M + + + + + + + + + + + +| 47 | Atrophy | 4 | 4 3.8| Hyperplasia, Lymphoid | 3 3 | 2 3.0| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Subcutaneous Tissue, Fibrosis | 3 | 1 3.0| Subcutaneous Tissue, Inflammation, | | | Chronic Active | 2 | 1 2.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 35 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 2| 3| 1| 2| 3| 2| 3| 2| 2| 3| 4| 2| 2| 1| 0| 3| 3| 2| 9| 3| 3| 2| 3| 3| | | 1| 9| 0| 6| 9| 0| 9| 0| 9| 9| 1| 5| 9| 9| 1| 8| 0| 0| 9| 5| 0| 1| 9| 0| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A | 10 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Skeletal Muscle | + | 2 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemorrhage | 4 | 1 4.0| Inflammation, Chronic Active | | 1 1.0| Necrosis | 4 | 1 4.0| Pigmentation | | 1 2.0| Alveolar Epithelium, Hyperplasia | 2 2 | 4 2.0| Alveolus, Infiltration Cellular, | | | Histiocyte | 2 4 | 4 2.5| |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | | 2 | Atrophy | | 1 4.0| Synechia | | 1 4.0| Retina, Degeneration | | 1 1.0| |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Hyperplasia | 2 2 2 | 6 2.2| Infiltration Cellular, Mononuclear | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 36 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 2| 3| 1| 2| 3| 2| 3| 2| 2| 3| 4| 2| 2| 1| 0| 3| 3| 2| 9| 3| 3| 2| 3| 3| | | 1| 9| 0| 6| 9| 0| 9| 0| 9| 9| 1| 5| 9| 9| 1| 8| 0| 0| 9| 5| 0| 1| 9| 0| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A | 10 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| SPECIAL SENSES SYSTEM - cont | | | Cell | 1 2 1 1 1 2 1 1 1 1 1 1 1 1 1 1| 33 1.2| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Mineralization | 1 1 | 2 1.0| Nephropathy | 1 1 1 1 | 10 1.1| Cortex, Cyst | 2 | 2 2.0| Cortex, Hydronephrosis | 4| 1 4.0| |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 37 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 3| 6| 7| 7| 6| 7| 3| 7| 6| 7| 5| 4| 6| 7| 7| 5| 7| 3| 3| 3| 7| 6| 1| 6| 7| DAY ON TEST | 8| 4| 3| 2| 6| 1| 8| 3| 4| 3| 8| 2| 4| 3| 2| 4| 2| 5| 5| 5| 3| 6| 8| 8| 3| | 2| 0| 1| 9| 0| 1| 6| 0| 2| 1| 0| 9| 9| 0| 9| 7| 9| 2| 2| 2| 0| 7| 5| 0| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 20 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Perforation | | |__________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Lymphocyte | | |__________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 2 2 2 2 2 2| Basophilic Focus | X X X | Clear Cell Focus | X X X | Eosinophilic Focus | X X X X X X | Hematopoietic Cell Proliferation | | Inflammation, Chronic Active | 1 1 1 2 1 1 1 1 1 1 1 1 1 1 1 1| Mineralization | 1 | Mixed Cell Focus | X X X X| Necrosis | 1 1 2 | Pigmentation | | Vacuolization Cytoplasmic | 1 1 2 2 1 2 2 1 1 1 2| Bile Duct, Cyst | 4 | |__________________________________________________________________________| Mesentery | + + + + + + | Fat, Inflammation, Chronic Active | 2 | Fat, Necrosis | 4 4 4 4 | |__________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 38 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 3| 6| 7| 7| 6| 7| 3| 7| 6| 7| 5| 4| 6| 7| 7| 5| 7| 3| 3| 3| 7| 6| 1| 6| 7| DAY ON TEST | 8| 4| 3| 2| 6| 1| 8| 3| 4| 3| 8| 2| 4| 3| 2| 4| 2| 5| 5| 5| 3| 6| 8| 8| 3| | 2| 0| 1| 9| 0| 1| 6| 0| 2| 1| 0| 9| 9| 0| 9| 7| 9| 2| 2| 2| 0| 7| 5| 0| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 20 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | |__________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 1 1 1 1 1 1 1 1| |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperkeratosis, Diffuse | 2 2 2 | Hyperkeratosis, Focal | 2 | Inflammation, Chronic Active, Focal | 2 | Epithelium, Hyperplasia, Focal | 2 1 1 2 2| |__________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Glands, Ectasia | 1 1 1 | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| Aorta, Mineralization | 2 | |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | 1 | Mineralization | 1 | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 | Hypertrophy | 1 | Inflammation, Chronic Active | 2 | Subcapsular, Hyperplasia | 1 1 2 2 1 2 2 2 2 2 1 2 2 2 2 2 2 1 1 1 3 1 2 2| |__________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Parathyroid Gland | M M + + + + + + + + + + M M + + + + + + + + + M +| |__________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 39 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 3| 6| 7| 7| 6| 7| 3| 7| 6| 7| 5| 4| 6| 7| 7| 5| 7| 3| 3| 3| 7| 6| 1| 6| 7| DAY ON TEST | 8| 4| 3| 2| 6| 1| 8| 3| 4| 3| 8| 2| 4| 3| 2| 4| 2| 5| 5| 5| 3| 6| 8| 8| 3| | 2| 0| 1| 9| 0| 1| 6| 0| 2| 1| 0| 9| 9| 0| 9| 7| 9| 2| 2| 2| 0| 7| 5| 0| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 20 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ENDOCRINE SYSTEM - cont | | Pars Distalis, Hyperplasia | 2 | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicle, Cyst | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Clitoral Gland | + + + M + + M + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | 1 | |__________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | 4 4 4 4 4 4 4 4 4 3 4 4 4 4 4 4 4 4| Cyst | 2 2 2 2 2 4 2 | Mineralization | 1 | Pigmentation | | Thrombosis | | |__________________________________________________________________________| Oviduct | + | |__________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 3 | Endometrium, Hyperplasia, Cystic | 3 3 4 1 1 1 4 | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Myelofibrosis | | Myeloid Cell, Hyperplasia | 3 4 | |__________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + +| Mediastinal, Hyperplasia, Lymphoid | | |__________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Hyperplasia, Lymphoid | 3 2 2 | __________________________________________|__________________________________________________________________________| Page 40 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 3| 6| 7| 7| 6| 7| 3| 7| 6| 7| 5| 4| 6| 7| 7| 5| 7| 3| 3| 3| 7| 6| 1| 6| 7| DAY ON TEST | 8| 4| 3| 2| 6| 1| 8| 3| 4| 3| 8| 2| 4| 3| 2| 4| 2| 5| 5| 5| 3| 6| 8| 8| 3| | 2| 0| 1| 9| 0| 1| 6| 0| 2| 1| 0| 9| 9| 0| 9| 7| 9| 2| 2| 2| 0| 7| 5| 0| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 20 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | |__________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + M + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | 3 | |__________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 2 4 2 2 2 4 4 2 2 | Hyperplasia, Lymphoid | 1 1| |__________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | 4 3 4 3 3 3 | Thymocyte, Necrosis | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skeletal Muscle | + | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Erythrophagocytosis | | Fibrosis | | Inflammation, Chronic Active | | Inflammation, Suppurative | | Pigmentation | 2 | Alveolus, Infiltration Cellular, | | __________________________________________|__________________________________________________________________________| Page 41 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 3| 6| 7| 7| 6| 7| 3| 7| 6| 7| 5| 4| 6| 7| 7| 5| 7| 3| 3| 3| 7| 6| 1| 6| 7| DAY ON TEST | 8| 4| 3| 2| 6| 1| 8| 3| 4| 3| 8| 2| 4| 3| 2| 4| 2| 5| 5| 5| 3| 6| 8| 8| 3| | 2| 0| 1| 9| 0| 1| 6| 0| 2| 1| 0| 9| 9| 0| 9| 7| 9| 2| 2| 2| 0| 7| 5| 0| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 20 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| RESPIRATORY SYSTEM - cont | | Histiocyte | 2 | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Nasolacrimal Duct, Inflammation, | | Suppurative | 1 | |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | + + + | Atrophy | 3 4 4 | Synechia | | Cornea, Inflammation, Chronic Active | | Lens, Cataract | 4 2 | |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + M + + + + + + + + + + + + +| Hyperplasia | 4 2 4 4 | Infiltration Cellular, Mononuclear | | Cell | 1 1 1 1 1 1 1 1 1 1 | Bilateral, Hyperplasia | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Metaplasia, Osseous | | Mineralization | 1 | Nephropathy | 1 1 1| Cortex, Cyst | | |__________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________ Page 42 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 1| 7| 5| 7| 7| 7| 1| 7| 7| 1| 7| 7| 7| 7| 7| 4| 6| 7| 7| 7| 5| 7| 7| 7| 5| | DAY ON TEST | 1| 2| 8| 3| 3| 2| 3| 3| 3| 3| 0| 2| 2| 2| 2| 9| 4| 3| 2| 2| 1| 2| 0| 2| 5| | | 3| 9| 2| 0| 0| 9| 5| 1| 0| 5| 4| 9| 9| 9| 9| 4| 9| 0| 9| 9| 3| 9| 4| 2| 5| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| A | 20 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Perforation | X | 1 | |__________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Lymphocyte | 2 | 1 2.0| |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | 2 3 | 8 2.1| Basophilic Focus | X X X | 6 | Clear Cell Focus | X X | 5 | Eosinophilic Focus | X X X X X | 11 | Hematopoietic Cell Proliferation | 1 | 1 1.0| Inflammation, Chronic Active | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 32 1.0| Mineralization | | 1 1.0| Mixed Cell Focus | X X X X X X X X X | 13 | Necrosis | 3 1 2 | 6 1.7| Pigmentation | 1 | 1 1.0| Vacuolization Cytoplasmic | 1 1 2 2 2 2 2 2 1 1 | 21 1.5| Bile Duct, Cyst | | 1 4.0| |__________________________________________________________________________|____________| Mesentery | + + + + | 10 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 43 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 1| 7| 5| 7| 7| 7| 1| 7| 7| 1| 7| 7| 7| 7| 7| 4| 6| 7| 7| 7| 5| 7| 7| 7| 5| | DAY ON TEST | 1| 2| 8| 3| 3| 2| 3| 3| 3| 3| 0| 2| 2| 2| 2| 9| 4| 3| 2| 2| 1| 2| 0| 2| 5| | | 3| 9| 2| 0| 0| 9| 5| 1| 0| 5| 4| 9| 9| 9| 9| 4| 9| 0| 9| 9| 3| 9| 4| 2| 5| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| A | 20 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | Fat, Inflammation, Chronic Active | | 1 2.0| Fat, Necrosis | 4 4 | 6 4.0| |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | 2 1 1 1 1 1 1 | 15 1.1| |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperkeratosis, Diffuse | | 3 2.0| Hyperkeratosis, Focal | | 1 2.0| Inflammation, Chronic Active, Focal | | 1 2.0| Epithelium, Hyperplasia, Focal | 1 2 | 7 1.6| |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Glands, Ectasia | 1 1 1 | 6 1.0| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Aorta, Mineralization | | 1 2.0| |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Chronic Active | 1 2 | 3 1.3| Mineralization | | 1 1.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | | 1 2.0| Hypertrophy | | 1 1.0| Inflammation, Chronic Active | | 1 2.0| Subcapsular, Hyperplasia | 1 2 2 2 3 2 1 3 2 1 2 2 3 3 1 1 2 2 2 2 1 2 2 2 2| 49 1.8| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 44 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 1| 7| 5| 7| 7| 7| 1| 7| 7| 1| 7| 7| 7| 7| 7| 4| 6| 7| 7| 7| 5| 7| 7| 7| 5| | DAY ON TEST | 1| 2| 8| 3| 3| 2| 3| 3| 3| 3| 0| 2| 2| 2| 2| 9| 4| 3| 2| 2| 1| 2| 0| 2| 5| | | 3| 9| 2| 0| 0| 9| 5| 1| 0| 5| 4| 9| 9| 9| 9| 4| 9| 0| 9| 9| 3| 9| 4| 2| 5| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| A | 20 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ENDOCRINE SYSTEM - cont | | | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Parathyroid Gland | M + M + + + + + + + + M + M M M M + M M + + M M +| 34 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + M| 49 | Pars Distalis, Hyperplasia | 2 | 2 2.0| |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Follicle, Cyst | 2 | 1 2.0| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | Inflammation, Chronic Active | | 1 1.0| |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Atrophy | 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4| 40 4.0| Cyst | 2 2 2 1 2 2 2 2 2 2 2 2 | 19 2.1| Mineralization | 2 | 2 1.5| Pigmentation | 2 2 | 2 2.0| Thrombosis | 4 4 | 2 4.0| |__________________________________________________________________________|____________| Oviduct | | 1 | |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | | 1 3.0| Endometrium, Hyperplasia, Cystic | 4 4 4 3 3 2 4 3 2 3 4 4 | 19 3.0| _____________________________________________________________________________________________________________________| | * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 45 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 1| 7| 5| 7| 7| 7| 1| 7| 7| 1| 7| 7| 7| 7| 7| 4| 6| 7| 7| 7| 5| 7| 7| 7| 5| | DAY ON TEST | 1| 2| 8| 3| 3| 2| 3| 3| 3| 3| 0| 2| 2| 2| 2| 9| 4| 3| 2| 2| 1| 2| 0| 2| 5| | | 3| 9| 2| 0| 0| 9| 5| 1| 0| 5| 4| 9| 9| 9| 9| 4| 9| 0| 9| 9| 3| 9| 4| 2| 5| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| A | 20 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Myelofibrosis | 2 | 1 2.0| Myeloid Cell, Hyperplasia | 4 4 4 | 5 3.8| |__________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + | 25 | Mediastinal, Hyperplasia, Lymphoid | 2 2 1 3 | 4 2.0| |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | 4 | 1 4.0| Hyperplasia, Lymphoid | 3 2 4 2 3 1 | 9 2.4| |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + M + + + + + M| 47 | Hyperplasia, Lymphoid | | 1 3.0| |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hematopoietic Cell Proliferation | 4 2 2 2 2 3 2 2 2 2 2 3| 21 2.5| Hyperplasia, Lymphoid | 1 1 2 1 3 1 1 3 | 10 1.5| |__________________________________________________________________________|____________| Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Atrophy | 3 4 3 3 | 10 3.3| Thymocyte, Necrosis | 4 | 1 4.0| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Chronic Active | 4 | 1 4.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 46 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 1| 7| 5| 7| 7| 7| 1| 7| 7| 1| 7| 7| 7| 7| 7| 4| 6| 7| 7| 7| 5| 7| 7| 7| 5| | DAY ON TEST | 1| 2| 8| 3| 3| 2| 3| 3| 3| 3| 0| 2| 2| 2| 2| 9| 4| 3| 2| 2| 1| 2| 0| 2| 5| | | 3| 9| 2| 0| 0| 9| 5| 1| 0| 5| 4| 9| 9| 9| 9| 4| 9| 0| 9| 9| 3| 9| 4| 2| 5| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| A | 20 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| MUSCULOSKELETAL SYSTEM - cont | | | Skeletal Muscle | | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Erythrophagocytosis | 2 | 1 2.0| Fibrosis | 2 | 1 2.0| Inflammation, Chronic Active | 2 | 1 2.0| Inflammation, Suppurative | 3 3 | 2 3.0| Pigmentation | | 1 2.0| Alveolus, Infiltration Cellular, | | | Histiocyte | 4 3 3 | 4 3.0| |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Nasolacrimal Duct, Inflammation, | | | Suppurative | | 1 1.0| |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + + + + + | 9 | Atrophy | 4 4 4 4 | 7 3.9| Synechia | 2 | 1 2.0| Cornea, Inflammation, Chronic Active | 2 2 3 2 4 | 5 2.6| Lens, Cataract | | 2 3.0| |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Hyperplasia | 3 4 4| 7 3.6| Infiltration Cellular, Mononuclear | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 47 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 1| 7| 5| 7| 7| 7| 1| 7| 7| 1| 7| 7| 7| 7| 7| 4| 6| 7| 7| 7| 5| 7| 7| 7| 5| | DAY ON TEST | 1| 2| 8| 3| 3| 2| 3| 3| 3| 3| 0| 2| 2| 2| 2| 9| 4| 3| 2| 2| 1| 2| 0| 2| 5| | | 3| 9| 2| 0| 0| 9| 5| 1| 0| 5| 4| 9| 9| 9| 9| 4| 9| 0| 9| 9| 3| 9| 4| 2| 5| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| A | 20 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| SPECIAL SENSES SYSTEM - cont | | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1 1 | 23 1.0| Bilateral, Hyperplasia | 3 | 1 3.0| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Metaplasia, Osseous | 1 | 1 1.0| Mineralization | 1 1 1 | 4 1.0| Nephropathy | 1 1 | 5 1.0| Cortex, Cyst | 2 | 1 2.0| |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 48 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 0| 3| 2| 8| 3| 3| 3| 3| 3| | 1| 2| 8| 3| 2| 1| 0| 2| 0| 1| 2| 3| 2| 2| 1| 1| 7| 1| 3| 2| 0| 1| 1| 0| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| Peyer's Patch, Hyperplasia, Lymphoid | 3 | |__________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 3 | Basophilic Focus | X | Clear Cell Focus | X X X X X X X X X X| Eosinophilic Focus | | Hematopoietic Cell Proliferation | | Infarct | 4 | Inflammation, Chronic Active | 1 1 1 1 1 2 1 1 1 1 1 | Mixed Cell Focus | X X X| Necrosis | 2 3 4 3 | Vacuolization Cytoplasmic | 2 1 2 2 2 2 2 2 1| Centrilobular, Degeneration | 3 | |__________________________________________________________________________| Mesentery | + + + + | Fat, Inflammation, Chronic Active | 2 | Fat, Necrosis | 4 | |__________________________________________________________________________| Oral Mucosa | + | Gingival, Foreign Body | X | Gingival, Inflammation, Suppurative | 2 | __________________________________________|__________________________________________________________________________| Page 49 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 0| 3| 2| 8| 3| 3| 3| 3| 3| | 1| 2| 8| 3| 2| 1| 0| 2| 0| 1| 2| 3| 2| 2| 1| 1| 7| 1| 3| 2| 0| 1| 1| 0| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | |__________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 1 | Inflammation, Chronic Active | | |__________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 2 1 2 1 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1| |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperkeratosis, Focal | | Inflammation, Chronic Active, Focal | | Epithelium, Hyperplasia, Focal | | |__________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | | Ulcer | 2 | Glands, Ectasia | 2 1 | |__________________________________________________________________________| Tooth | + + + + | Malformation | X X X X | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | | Mineralization | 2 | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Hypertrophy | 1 1 2 2 1 1 2 2 1 2 2 | Subcapsular, Cyst Multilocular | | Subcapsular, Hyperplasia | 2 2 1 2 1 2 2 2 2 2 1 2 2 2 1 2 2 1 2 1 2 1| |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 50 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 0| 3| 2| 8| 3| 3| 3| 3| 3| | 1| 2| 8| 3| 2| 1| 0| 2| 0| 1| 2| 3| 2| 2| 1| 1| 7| 1| 3| 2| 0| 1| 1| 0| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ENDOCRINE SYSTEM - cont | | Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Parathyroid Gland | + + + + + M + + + + M + + + + + + M + M + + M M +| Cyst | 1 1 | |__________________________________________________________________________| Pituitary Gland | + + + + + + + + M + + + + + + + + + + + + + + + +| Pars Distalis, Cyst | | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicle, Cyst | 2 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Coagulating Gland | + | |__________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | 3 | Granuloma Sperm | 2 | Infiltration Cellular, Mononuclear | | Cell | 1 1 1 1 1 1 1 1 1 1 2 1 1 1 1 1 1 1 1 1 1| Inflammation, Chronic Active | 2 | Mineralization | | |__________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + I + + + +| Infiltration Cellular, Mononuclear | | Cell | 1 1 2 2 1| Inflammation, Chronic Active | 2 1 1 2 | Bilateral, Inflammation, Chronic | | Active | 2 | Bilateral, Duct, Ectasia | 3 3 2 | Duct, Ectasia | 2 2 2 2 3 2 | |__________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 51 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 0| 3| 2| 8| 3| 3| 3| 3| 3| | 1| 2| 8| 3| 2| 1| 0| 2| 0| 1| 2| 3| 2| 2| 1| 1| 7| 1| 3| 2| 0| 1| 1| 0| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| GENITAL SYSTEM - cont | | Infiltration Cellular, Mononuclear | | Cell | 1 1 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1| Inflammation, Chronic Active | 1 2 | |__________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | | |__________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Mineralization | 3 1 | Necrosis | 4 | Germinal Epithelium, Degeneration | 2 | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Lymph Node | + + + | |__________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + M + + + +| Hyperplasia, Lymphoid | | |__________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 2 | Hyperplasia, Lymphoid | 4 3 | Inflammation, Chronic Active | 2 | |__________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Cellular | | Hematopoietic Cell Proliferation | 2 4 2 3 4 4 2 3 | Hyperplasia, Lymphoid | 2 2 | |__________________________________________________________________________| Thymus | + + + + + + + + + + + + M + M + + + + + + + + + +| Atrophy | 4 3 3 3 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | M M M M + M M M M M M M M M M M M M + M M M M M +| |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 52 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 0| 3| 2| 8| 3| 3| 3| 3| 3| | 1| 2| 8| 3| 2| 1| 0| 2| 0| 1| 2| 3| 2| 2| 1| 1| 7| 1| 3| 2| 0| 1| 1| 0| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| INTEGUMENTARY SYSTEM - cont | | Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 1 | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skeletal Muscle | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Peripheral Nerve | + | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Erythrophagocytosis | | Inflammation, Chronic Active | 1 | Metaplasia, Osseous | | Necrosis | | Pigmentation | | Alveolar Epithelium, Hyperplasia | 2 2 | Alveolus, Infiltration Cellular, | | Histiocyte | | Mediastinum, Inflammation, Chronic | | Active | | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Suppurative | 2 | Nasolacrimal Duct, Inflammation, | | Suppurative | 2 2 | |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | __________________________________________|__________________________________________________________________________| Page 53 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 0| 3| 2| 8| 3| 3| 3| 3| 3| | 1| 2| 8| 3| 2| 1| 0| 2| 0| 1| 2| 3| 2| 2| 1| 1| 7| 1| 3| 2| 0| 1| 1| 0| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| SPECIAL SENSES SYSTEM - cont | | |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | | Infiltration Cellular, Mononuclear | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Infarct | 2 | Inflammation, Chronic Active | | Metaplasia, Osseous | 1 1 1 | Mineralization | 1 1 1 1 1 1 1 2 1 1 1 1 1| Nephropathy | 1 1 1 2 1 1 1 1 1 1 2 2 2 1 1 1 1 1 1 1 1| Cortex, Cyst | 2 2 2 | Renal Tubule, Hyperplasia | 1 1 1 | |__________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________ Page 54 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 4| 7| 7| 7| 7| 6| 6| 7| | DAY ON TEST | 3| 3| 1| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 6| 3| 3| 3| 3| 1| 6| 3| | | 1| 2| 2| 4| 2| 0| 1| 2| 1| 2| 1| 2| 1| 1| 2| 2| 6| 4| 1| 2| 1| 1| 7| 4| 1| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Peyer's Patch, Hyperplasia, Lymphoid | 3 | 2 3.0| |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | | 1 3.0| Basophilic Focus | | 1 | Clear Cell Focus | X X X X X X X X X| 19 | Eosinophilic Focus | X X X X X | 5 | Hematopoietic Cell Proliferation | 1 1 | 2 1.0| Infarct | 4 | 2 4.0| Inflammation, Chronic Active | 1 1 1 1 2 1 1 2 1 1 1 1 1 1| 25 1.1| Mixed Cell Focus | X X X X X X | 9 | Necrosis | | 4 3.0| Vacuolization Cytoplasmic | 2 2 2 1 2 2 1 2 2 | 18 1.8| Centrilobular, Degeneration | | 1 3.0| |__________________________________________________________________________|____________| Mesentery | + + + + | 8 | Fat, Inflammation, Chronic Active | | 1 2.0| Fat, Necrosis | 4 | 2 4.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 55 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 4| 7| 7| 7| 7| 6| 6| 7| | DAY ON TEST | 3| 3| 1| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 6| 3| 3| 3| 3| 1| 6| 3| | | 1| 2| 2| 4| 2| 0| 1| 2| 1| 2| 1| 2| 1| 1| 2| 2| 6| 4| 1| 2| 1| 1| 7| 4| 1| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | |__________________________________________________________________________|____________| Oral Mucosa | + | 2 | Gingival, Foreign Body | X | 2 | Gingival, Inflammation, Suppurative | 2 | 2 2.0| |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | | 1 1.0| Inflammation, Chronic Active | 2 | 1 2.0| |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 35 1.1| |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperkeratosis, Focal | 3 2 | 2 2.5| Inflammation, Chronic Active, Focal | 2 | 1 2.0| Epithelium, Hyperplasia, Focal | 3 3 | 2 3.0| |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia, Focal | 2 | 1 2.0| Ulcer | | 1 2.0| Glands, Ectasia | | 2 1.5| |__________________________________________________________________________|____________| Tooth | + + + + + | 9 | Malformation | X X X X X | 9 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Chronic Active | 1 | 1 1.0| Mineralization | 2 | 2 2.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 56 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 4| 7| 7| 7| 7| 6| 6| 7| | DAY ON TEST | 3| 3| 1| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 6| 3| 3| 3| 3| 1| 6| 3| | | 1| 2| 2| 4| 2| 0| 1| 2| 1| 2| 1| 2| 1| 1| 2| 2| 6| 4| 1| 2| 1| 1| 7| 4| 1| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hypertrophy | 2 2 1 2 1 2 2 2| 19 1.6| Subcapsular, Cyst Multilocular | 2 | 1 2.0| Subcapsular, Hyperplasia | 1 2 1 1 1 2 1 1 1 1 2 2 1 1 1 1 2 2 2 2 1 1 2| 45 1.5| |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + + + + + + + + + M M + + + +| 42 | Cyst | | 2 1.0| |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Pars Distalis, Cyst | 2 1 1| 3 1.3| |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Follicle, Cyst | 2 2 | 3 2.0| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Coagulating Gland | | 1 | |__________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Atrophy | | 1 3.0| Granuloma Sperm | | 1 2.0| Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 1 1 1 1 1 1 1 2 1 1 1 1 1 1 1 1 1 | 42 1.0| Inflammation, Chronic Active | 2 | 2 2.0| Mineralization | 1 | 1 1.0| |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 57 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 4| 7| 7| 7| 7| 6| 6| 7| | DAY ON TEST | 3| 3| 1| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 6| 3| 3| 3| 3| 1| 6| 3| | | 1| 2| 2| 4| 2| 0| 1| 2| 1| 2| 1| 2| 1| 1| 2| 2| 6| 4| 1| 2| 1| 1| 7| 4| 1| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 1 1 | 11 1.2| Inflammation, Chronic Active | 2 2 4 2| 8 2.0| Bilateral, Inflammation, Chronic | | | Active | | 1 2.0| Bilateral, Duct, Ectasia | 2 | 4 2.5| Duct, Ectasia | 3 3 2 2 2 2 2 | 13 2.2| |__________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 36 1.0| Inflammation, Chronic Active | | 2 1.5| |__________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Chronic Active | 3 | 1 3.0| |__________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Mineralization | | 2 2.0| Necrosis | | 1 4.0| Germinal Epithelium, Degeneration | 4 | 2 3.0| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Lymph Node | + + + | 6 | |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Hyperplasia, Lymphoid | 2 | 1 2.0| |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + M + + + + + + + +| 48 | Hematopoietic Cell Proliferation | | 1 2.0| Hyperplasia, Lymphoid | 2 3 | 4 3.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 58 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 4| 7| 7| 7| 7| 6| 6| 7| | DAY ON TEST | 3| 3| 1| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 6| 3| 3| 3| 3| 1| 6| 3| | | 1| 2| 2| 4| 2| 0| 1| 2| 1| 2| 1| 2| 1| 1| 2| 2| 6| 4| 1| 2| 1| 1| 7| 4| 1| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | Inflammation, Chronic Active | | 1 2.0| |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Depletion Cellular | 3 | 1 3.0| Hematopoietic Cell Proliferation | 2 2 3 2 3 2 3 2 4 | 17 2.8| Hyperplasia, Lymphoid | | 2 2.0| |__________________________________________________________________________|____________| Thymus | + + + + + + + + + + + + + + + + + + + M + + + + +| 47 | Atrophy | 3 3 3 | 7 3.1| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| 3 | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | | 1 1.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Skeletal Muscle | + | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Peripheral Nerve | | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Erythrophagocytosis | 3 | 1 3.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 59 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 4| 7| 7| 7| 7| 6| 6| 7| | DAY ON TEST | 3| 3| 1| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 6| 3| 3| 3| 3| 1| 6| 3| | | 1| 2| 2| 4| 2| 0| 1| 2| 1| 2| 1| 2| 1| 1| 2| 2| 6| 4| 1| 2| 1| 1| 7| 4| 1| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| RESPIRATORY SYSTEM - cont | | | Inflammation, Chronic Active | 2 4 1 | 4 2.0| Metaplasia, Osseous | 1 | 1 1.0| Necrosis | 2 | 1 2.0| Pigmentation | 1 | 1 1.0| Alveolar Epithelium, Hyperplasia | 2 2 | 4 2.0| Alveolus, Infiltration Cellular, | | | Histiocyte | 1 | 1 1.0| Mediastinum, Inflammation, Chronic | | | Active | 2 | 1 2.0| |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Suppurative | | 1 2.0| Nasolacrimal Duct, Inflammation, | | | Suppurative | 3 2 | 4 2.3| |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | 2 | 1 2.0| Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 33 1.0| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infarct | 2 2 3 2 | 5 2.2| Inflammation, Chronic Active | 2 2 | 2 2.0| Metaplasia, Osseous | 1 2 | 5 1.2| Mineralization | 1 1 1 1 1 1 1 1 1 1| 23 1.0| Nephropathy | 1 1 1 1 2 1 1 2 2 1 2 1 1 1 1 1 1 1 1 1| 41 1.2| Cortex, Cyst | 2 1 2 1 1 2 2| 10 1.7| Renal Tubule, Hyperplasia | 2 2 | 5 1.4| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 60 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 4| 7| 7| 7| 7| 6| 6| 7| | DAY ON TEST | 3| 3| 1| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 6| 3| 3| 3| 3| 1| 6| 3| | | 1| 2| 2| 4| 2| 0| 1| 2| 1| 2| 1| 2| 1| 1| 2| 2| 6| 4| 1| 2| 1| 1| 7| 4| 1| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| URINARY SYSTEM - cont | | | |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 61 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 0| 7| 7| 7| 0| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 1| 3| 3| 3| 3| 3| 0| 3| 3| 3| 0| 3| 3| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 1| 2| 4| 1| 2| 1| 2| 1| 4| 1| 1| 2| 3| 1| 2| 9| 1| 1| 0| 1| 1| 0| 0| 2| 0| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Suppurative | 3 | Perforation | X X | |__________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + + + M + + + +| |__________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| Ulcer | 4 | |__________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| Peyer's Patch, Hyperplasia, Lymphoid | | |__________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Basophilic Focus | X X X X| Clear Cell Focus | X X X X X X X X X X X X X X| Eosinophilic Focus | X X X X| Hematopoietic Cell Proliferation | 1 | Infarct | 4 | Inflammation, Chronic Active | 2 1 1 2 1 1 1 1 1 1 1 1 1| Mineralization | | Mixed Cell Focus | X X X X X X | Necrosis | 1 1 2 1 | Pigmentation | 2 2 | Vacuolization Cytoplasmic | 1 1 2 3 2 3 2 2 2 1 2 2 3 1 2 1| |__________________________________________________________________________| Mesentery | + + + | Fat, Necrosis | 4 | |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 62 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 0| 7| 7| 7| 0| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 1| 3| 3| 3| 3| 3| 0| 3| 3| 3| 0| 3| 3| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 1| 2| 4| 1| 2| 1| 2| 1| 4| 1| 1| 2| 3| 1| 2| 9| 1| 1| 0| 1| 1| 0| 0| 2| 0| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 1 | Acinus, Atrophy | | |__________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1 1 | |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperkeratosis, Focal | 2 3 | Inflammation, Chronic Active, Focal | 2 3 | Inflammation, Chronic Active, | | Multifocal | | Ulcer | 4 | Ulcer, Multifocal | 3 | Epithelium, Hyperplasia, Focal | 3 3 | |__________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | 2 | Inflammation, Chronic Active | 2 | Glands, Ectasia | 1 2 | |__________________________________________________________________________| Tooth | + + + + | Malformation | X X X X | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | 2 | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Hypertrophy | 2 2 2 2 2 1 1 2 1 1 1| Subcapsular, Hyperplasia | 1 2 2 1 1 2 2 2 2 1 1 2 2 1 2 2 2 3 2 2 1| __________________________________________|__________________________________________________________________________| Page 63 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 0| 7| 7| 7| 0| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 1| 3| 3| 3| 3| 3| 0| 3| 3| 3| 0| 3| 3| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 1| 2| 4| 1| 2| 1| 2| 1| 4| 1| 1| 2| 3| 1| 2| 9| 1| 1| 0| 1| 1| 0| 0| 2| 0| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ENDOCRINE SYSTEM - cont | | |__________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | | |__________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Parathyroid Gland | + + + + + + + + M + + + M + M M + + M + + + + + +| |__________________________________________________________________________| Pituitary Gland | M + + + + + M + + + + + + + + + + + + M + + + + +| Pars Distalis, Cyst | 2 | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicle, Cyst | 2 2 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Granuloma Sperm | | Infiltration Cellular, Mononuclear | | Cell | 2 1 1 1 1 1 1 1 1 1 1 1 1 | |__________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 1 1 | Inflammation, Chronic Active | 1 3 | Bilateral, Duct, Ectasia | | Duct, Ectasia | 2 2 2 2 2 3 | |__________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 2 1 1 1 1 1 1 1 1 2 1 1 1 1| Inflammation, Chronic Active | | |__________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 64 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 0| 7| 7| 7| 0| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 1| 3| 3| 3| 3| 3| 0| 3| 3| 3| 0| 3| 3| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 1| 2| 4| 1| 2| 1| 2| 1| 4| 1| 1| 2| 3| 1| 2| 9| 1| 1| 0| 1| 1| 0| 0| 2| 0| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| GENITAL SYSTEM - cont | | Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Mineralization | | Germinal Epithelium, Degeneration | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Myeloid Cell, Hyperplasia | | |__________________________________________________________________________| Lymph Node | + + + | Mediastinal, Hyperplasia, Lymphoid | | |__________________________________________________________________________| Lymph Node, Mandibular | + + M + + + + + + + + + + + + M + + M + + + + + +| Hematopoietic Cell Proliferation | 3 | Hyperplasia, Lymphoid | | |__________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + M + + + + + + + + +| Hyperplasia, Lymphoid | | Hyperplasia, Plasma Cell | | |__________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 4 2 | Red Pulp, Depletion Cellular | | |__________________________________________________________________________| Thymus | + + M + + + + M + + + + + + + + + + + + + + + + +| Atrophy | | Inflammation, Chronic Active | 2 | Thymocyte, Necrosis | 4 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Infiltration | | Cellular, Mast Cell | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | __________________________________________|__________________________________________________________________________| Page 65 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 0| 7| 7| 7| 0| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 1| 3| 3| 3| 3| 3| 0| 3| 3| 3| 0| 3| 3| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 1| 2| 4| 1| 2| 1| 2| 1| 4| 1| 1| 2| 3| 1| 2| 9| 1| 1| 0| 1| 1| 0| 0| 2| 0| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| MUSCULOSKELETAL SYSTEM - cont | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperostosis | 3 | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemorrhage | 2 | Inflammation, Chronic Active | | Thrombosis | | Vacuolization Cytoplasmic | 3 | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | | Inflammation, Suppurative | 3 | Alveolar Epithelium, Hyperplasia | 4 | Mediastinum, Hemorrhage | 3 | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Nasolacrimal Duct, Inflammation, | | Suppurative | | |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | | Atrophy | | Lens, Cataract | | |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 | Infiltration Cellular, Mononuclear | | Cell | 1 1 1 1 1 1 1 | |__________________________________________________________________________| Zymbal's Gland | + | __________________________________________|__________________________________________________________________________| Page 66 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 0| 7| 7| 7| 0| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 1| 3| 3| 3| 3| 3| 0| 3| 3| 3| 0| 3| 3| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 1| 2| 4| 1| 2| 1| 2| 1| 4| 1| 1| 2| 3| 1| 2| 9| 1| 1| 0| 1| 1| 0| 0| 2| 0| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Infarct | | Inflammation, Chronic Active | 2 | Inflammation, Suppurative | | Metaplasia, Osseous | | Mineralization | 1 1 1 1 1 1 1 1 1 1 1 1 1| Necrosis | | Nephropathy | 1 1 1 2 1 1 1 1 1 1 1 1 1 1 1 1 2 1 1| Artery, Inflammation, Chronic Active | | Cortex, Cyst | 1 2 2 | Pelvis, Inflammation, Suppurative | | Renal Tubule, Hyperplasia | 2 | |__________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________ Page 67 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 6| 5| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 6| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 8| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | 2| 2| 0| 2| 1| 1| 9| 8| 1| 1| 0| 0| 2| 2| 2| 5| 1| 0| 2| 7| 2| 2| 1| 0| 2| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Suppurative | | 1 3.0| Perforation | | 2 | |__________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Ulcer | | 1 4.0| |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Peyer's Patch, Hyperplasia, Lymphoid | 3 | 1 3.0| |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | 3 3 | 2 3.0| Basophilic Focus | X | 5 | Clear Cell Focus | X X X X X X X X X X | 24 | Eosinophilic Focus | X | 5 | Hematopoietic Cell Proliferation | 1 | 2 1.0| Infarct | | 1 4.0| Inflammation, Chronic Active | 1 1 1 2 1 2 2 1 1 1 2 1 1 1 2 1 1 1 1 1| 33 1.2| Mineralization | 1 | 1 1.0| Mixed Cell Focus | X X X X X X X | 13 | Necrosis | 2 2 2 3 2 2 | 10 1.8| Pigmentation | 2 1 | 4 1.8| Vacuolization Cytoplasmic | 2 2 2 2 1 1 3 2 2 2 2 2 2 1 | 30 1.9| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 68 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 6| 5| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 6| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 8| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | 2| 2| 0| 2| 1| 1| 9| 8| 1| 1| 0| 0| 2| 2| 2| 5| 1| 0| 2| 7| 2| 2| 1| 0| 2| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | |__________________________________________________________________________|____________| Mesentery | + + + | 6 | Fat, Necrosis | 4 4 | 3 4.0| |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | 1 | 2 1.0| Acinus, Atrophy | 4 | 1 4.0| |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 34 1.0| |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperkeratosis, Focal | 1 | 3 2.0| Inflammation, Chronic Active, Focal | | 2 2.5| Inflammation, Chronic Active, | | | Multifocal | 1 | 1 1.0| Ulcer | | 1 4.0| Ulcer, Multifocal | 2 | 2 2.5| Epithelium, Hyperplasia, Focal | 1 2 | 4 2.3| |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia, Focal | | 1 2.0| Inflammation, Chronic Active | 2 | 2 2.0| Glands, Ectasia | 2 1 1 1 1| 7 1.3| |__________________________________________________________________________|____________| Tooth | + | 5 | Malformation | X | 5 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 69 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 6| 5| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 6| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 8| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | 2| 2| 0| 2| 1| 1| 9| 8| 1| 1| 0| 0| 2| 2| 2| 5| 1| 0| 2| 7| 2| 2| 1| 0| 2| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| CARDIOVASCULAR SYSTEM - cont | | | Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Chronic Active | 1 2| 3 1.7| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hypertrophy | 2 1 2 3 1 1 2 2 1 2 | 21 1.6| Subcapsular, Hyperplasia | 1 1 1 2 1 1 1 1 2 2 1 2 1 1 1 1 2 2 1 1 1 2| 43 1.5| |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | 2 | 1 2.0| |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + + + + + M + + + + + + + + +| 44 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + M + + + + + + + + + + +| 46 | Pars Distalis, Cyst | 1 | 2 1.5| |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Follicle, Cyst | 3 | 3 2.3| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Granuloma Sperm | 2 | 1 2.0| Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 1 1 2 1 1 1 1 1 1 1 2 1 1 1 | 31 1.1| |__________________________________________________________________________|____________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 70 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 6| 5| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 6| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 8| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | 2| 2| 0| 2| 1| 1| 9| 8| 1| 1| 0| 0| 2| 2| 2| 5| 1| 0| 2| 7| 2| 2| 1| 0| 2| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | Cell | 1 1 1 1 1 2 1 2 2 1 1 | 13 1.2| Inflammation, Chronic Active | 2 | 3 2.0| Bilateral, Duct, Ectasia | 2 | 1 2.0| Duct, Ectasia | 3 2 3 3 | 10 2.4| |__________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 1 1 1 1 1 1 1 2 1 1 1 1 1 | 31 1.1| Inflammation, Chronic Active | 2 2 | 2 2.0| |__________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Mineralization | 1 | 1 1.0| Germinal Epithelium, Degeneration | 1 | 1 1.0| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Myeloid Cell, Hyperplasia | 4 | 1 4.0| |__________________________________________________________________________|____________| Lymph Node | + | 4 | Mediastinal, Hyperplasia, Lymphoid | 3 | 1 3.0| |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| 47 | Hematopoietic Cell Proliferation | | 1 3.0| Hyperplasia, Lymphoid | 2 | 1 2.0| |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + M + + + + + + + + +| 48 | Hyperplasia, Lymphoid | 3 | 1 3.0| Hyperplasia, Plasma Cell | 3 | 1 3.0| |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hematopoietic Cell Proliferation | 2 3 3 2 2 | 7 2.6| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 71 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 6| 5| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 6| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 8| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | 2| 2| 0| 2| 1| 1| 9| 8| 1| 1| 0| 0| 2| 2| 2| 5| 1| 0| 2| 7| 2| 2| 1| 0| 2| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | Red Pulp, Depletion Cellular | 3 | 1 3.0| |__________________________________________________________________________|____________| Thymus | + + + + + M + + + + + + + + + + + + + + + + + + +| 47 | Atrophy | 3 3 4 4 | 4 3.5| Inflammation, Chronic Active | | 1 2.0| Thymocyte, Necrosis | | 1 4.0| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Subcutaneous Tissue, Infiltration | | | Cellular, Mast Cell | 2 | 1 2.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperostosis | | 1 3.0| _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemorrhage | 2 | 2 2.0| Inflammation, Chronic Active | 2 | 1 2.0| Thrombosis | 3 | 1 3.0| Vacuolization Cytoplasmic | | 1 3.0| _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Chronic Active | 1 | 1 1.0| Inflammation, Suppurative | 1 | 2 2.0| Alveolar Epithelium, Hyperplasia | 4 1 | 3 3.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 72 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 6| 5| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 6| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 8| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | 2| 2| 0| 2| 1| 1| 9| 8| 1| 1| 0| 0| 2| 2| 2| 5| 1| 0| 2| 7| 2| 2| 1| 0| 2| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| RESPIRATORY SYSTEM - cont | | | Mediastinum, Hemorrhage | | 1 3.0| |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Nasolacrimal Duct, Inflammation, | | | Suppurative | 3 | 1 3.0| |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + | 1 | Atrophy | 4 | 1 4.0| Lens, Cataract | 2 | 1 2.0| |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | 1 2 4 | 4 2.3| Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 25 1.0| |__________________________________________________________________________|____________| Zymbal's Gland | | 1 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infarct | 2 2 2 | 3 2.0| Inflammation, Chronic Active | 1 1| 3 1.3| Inflammation, Suppurative | 3 | 1 3.0| Metaplasia, Osseous | 2 2 | 2 2.0| Mineralization | 1 1 1 1 1 1 1 1 1 2 1 1 1 1 1| 28 1.0| Necrosis | 2 | 1 2.0| Nephropathy | 1 3 1 1 2 1 1 1 1 1 1 1 1 1 1 1 2 2 1 1| 39 1.2| Artery, Inflammation, Chronic Active | 2 | 1 2.0| Cortex, Cyst | 2 2 2 2 2 2 3 3 1 1 | 13 1.9| Pelvis, Inflammation, Suppurative | 1 | 1 1.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 73 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 6| 5| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 6| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 8| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | 2| 2| 0| 2| 1| 1| 9| 8| 1| 1| 0| 0| 2| 2| 2| 5| 1| 0| 2| 7| 2| 2| 1| 0| 2| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| URINARY SYSTEM - cont | | | Renal Tubule, Hyperplasia | 1 2 2 2 | 5 1.8| |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 74 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 6| 7| 2| 7| 5| 7| DAY ON TEST | 3| 3| 1| 1| 3| 3| 3| 3| 3| 3| 0| 1| 3| 3| 3| 3| 3| 3| 3| 8| 3| 1| 3| 1| 3| | 0| 2| 5| 4| 2| 2| 1| 2| 1| 1| 9| 0| 2| 2| 0| 1| 0| 1| 1| 5| 1| 1| 2| 2| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 10 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | 3 | Ulcer | 4 | |__________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | | |__________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 3 2 | Basophilic Focus | X X X X| Clear Cell Focus | X X X X X X | Eosinophilic Focus | X X X X X | Fibrosis | 2 | Hematopoietic Cell Proliferation | | Infarct | 4 | Inflammation, Chronic Active | 2 1 1 1 1 1 1 2 1 1 1 2 2 2| Mineralization | 1 | Mixed Cell Focus | X X X X X X X | Necrosis | 3 2 | Pigmentation | 1 1| Vacuolization Cytoplasmic | 2 3 2 2 1 1 2 2 | |__________________________________________________________________________| Mesentery | + + + + | Artery, Inflammation, Chronic Active | | Artery, Necrosis, Fibrinoid | | __________________________________________|__________________________________________________________________________| Page 75 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 6| 7| 2| 7| 5| 7| DAY ON TEST | 3| 3| 1| 1| 3| 3| 3| 3| 3| 3| 0| 1| 3| 3| 3| 3| 3| 3| 3| 8| 3| 1| 3| 1| 3| | 0| 2| 5| 4| 2| 2| 1| 2| 1| 1| 9| 0| 2| 2| 0| 1| 0| 1| 1| 5| 1| 1| 2| 2| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 10 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | Fat, Mineralization | 3 | Fat, Necrosis | 4 4 | |__________________________________________________________________________| Oral Mucosa | + +| Gingival, Foreign Body | X X| Gingival, Inflammation, Suppurative | 2 2| |__________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 2 | |__________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| Duct, Cyst | 2 | |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperkeratosis, Diffuse | 2 2 | Hyperkeratosis, Focal | 1 2 1 | Inflammation, Chronic Active | | Inflammation, Chronic Active, | | Multifocal | | Ulcer | | Ulcer, Multifocal | | Epithelium, Hyperplasia, Focal | 2 1 1 1 | |__________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Mineralization | 2 | Glands, Ectasia | 1 1 1 1 1 1 | |__________________________________________________________________________| Tooth | | Inflammation, Suppurative | | Malformation | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| Aorta, Mineralization | 2 | __________________________________________|__________________________________________________________________________| Page 76 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 6| 7| 2| 7| 5| 7| DAY ON TEST | 3| 3| 1| 1| 3| 3| 3| 3| 3| 3| 0| 1| 3| 3| 3| 3| 3| 3| 3| 8| 3| 1| 3| 1| 3| | 0| 2| 5| 4| 2| 2| 1| 2| 1| 1| 9| 0| 2| 2| 0| 1| 0| 1| 1| 5| 1| 1| 2| 2| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 10 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | 1 | Inflammation, Suppurative | 2 | Valve, Inflammation, Chronic Active | 1 | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Hypertrophy | 1 1 2 1 1 2 1 1 1| Subcapsular, Hyperplasia | 1 1 1 1 1 2 1 1 1 2 2 2 2 1 1 2 1 2 1 | |__________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | | |__________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Parathyroid Gland | M + M + + M + + M M + + + + + M + + + + + + + M +| |__________________________________________________________________________| Pituitary Gland | + + + + + + + M + + + + + + + + + + + M + + + + +| Pars Distalis, Cyst | 2 1 | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicle, Cyst | 2 2 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Coagulating Gland | + + | |__________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| Inflammation, Chronic Active | | Inflammation, Suppurative | 2 | |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 77 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 6| 7| 2| 7| 5| 7| DAY ON TEST | 3| 3| 1| 1| 3| 3| 3| 3| 3| 3| 0| 1| 3| 3| 3| 3| 3| 3| 3| 8| 3| 1| 3| 1| 3| | 0| 2| 5| 4| 2| 2| 1| 2| 1| 1| 9| 0| 2| 2| 0| 1| 0| 1| 1| 5| 1| 1| 2| 2| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 10 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| GENITAL SYSTEM - cont | | Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Basal Cell | 2 | Infiltration Cellular, Mononuclear | | Cell | 1 1 1 2 1 1 2 | Inflammation, Chronic Active | 3 2 3 1 1 | Bilateral, Duct, Ectasia | 2 2 | Duct, Ectasia | 2 2 3 2 2 2 2 2| |__________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 1 1 1 1 2 1 1 1 1 1 1 1 1 1 1 1| Mineralization | | |__________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Suppurative | 3 | Mineralization | 1 | Germinal Epithelium, Degeneration | 2 2 | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 3 3 | Atrophy | 2 | Inflammation, Suppurative | 2 | Myeloid Cell, Hyperplasia | 4 | |__________________________________________________________________________| Lymph Node | + + + | Mediastinal, Hyperplasia, Lymphoid | 3 | |__________________________________________________________________________| Lymph Node, Mandibular | + + + M + + + M + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | 2 2 2 | |__________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 3 3 4 2 2 3 3 2 4 2 2| __________________________________________|__________________________________________________________________________| Page 78 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 6| 7| 2| 7| 5| 7| DAY ON TEST | 3| 3| 1| 1| 3| 3| 3| 3| 3| 3| 0| 1| 3| 3| 3| 3| 3| 3| 3| 8| 3| 1| 3| 1| 3| | 0| 2| 5| 4| 2| 2| 1| 2| 1| 1| 9| 0| 2| 2| 0| 1| 0| 1| 1| 5| 1| 1| 2| 2| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 10 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | |__________________________________________________________________________| Thymus | + + + M + + + + + + + + M + + + + + + + + + M + +| Atrophy | 4 4 2 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 1 | Inflammation, Chronic Active | 3 | Ulcer | 4 | Subcutaneous Tissue, Inflammation, | | Suppurative | 2 | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skeletal Muscle | + | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Cyst Epithelial Inclusion | | |__________________________________________________________________________| Peripheral Nerve | + | |__________________________________________________________________________| Spinal Cord | + | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Erythrophagocytosis | 2 | Inflammation, Chronic Active | 2 2 2 | Mineralization | | Pigmentation | 1 | __________________________________________|__________________________________________________________________________| Page 79 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 6| 7| 2| 7| 5| 7| DAY ON TEST | 3| 3| 1| 1| 3| 3| 3| 3| 3| 3| 0| 1| 3| 3| 3| 3| 3| 3| 3| 8| 3| 1| 3| 1| 3| | 0| 2| 5| 4| 2| 2| 1| 2| 1| 1| 9| 0| 2| 2| 0| 1| 0| 1| 1| 5| 1| 1| 2| 2| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 10 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| RESPIRATORY SYSTEM - cont | | Alveolar Epithelium, Hyperplasia | 4 2 2 | Alveolus, Infiltration Cellular, | | Histiocyte | 2 3 | Artery, Mediastinum, Mineralization | 3 | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Foreign Body | X | Inflammation, Suppurative | 2 | Nasolacrimal Duct, Inflammation, | | Suppurative | 2 3 1 | |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | + + | Atrophy | 4 | Synechia | | Cornea, Inflammation, Chronic Active | | Cornea, Sclera, Inflammation, | | Chronic Active | | Lens, Cataract | 2 | Retina, Atrophy | | |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 3 4 4 4 | Infiltration Cellular, Mononuclear | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1| _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Accumulation, Hyaline Droplet | | Infarct | 2 2 | Inflammation, Chronic Active | 2| Metaplasia, Osseous | 2 2 2 | Mineralization | 1 1 1 1 1 1 1 1 1 1 1 1 1 1| Nephropathy | 1 1 1 2 1 1 2 1 4 1 1 1 1 1 2 2 1 1 1 1 1| __________________________________________|__________________________________________________________________________| Page 80 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 6| 7| 2| 7| 5| 7| DAY ON TEST | 3| 3| 1| 1| 3| 3| 3| 3| 3| 3| 0| 1| 3| 3| 3| 3| 3| 3| 3| 8| 3| 1| 3| 1| 3| | 0| 2| 5| 4| 2| 2| 1| 2| 1| 1| 9| 0| 2| 2| 0| 1| 0| 1| 1| 5| 1| 1| 2| 2| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 10 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| URINARY SYSTEM - cont | | Cortex, Cyst | 2 1 2 2 2 1 2 | Glomerulus, Thrombosis | | Renal Tubule, Casts Protein | 2 | Renal Tubule, Hyperplasia | 1 | |__________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________ Page 81 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 4| 7| 5| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 9| 3| 6| 3| | | 1| 2| 2| 0| 1| 0| 2| 0| 2| 1| 2| 2| 0| 2| 0| 2| 2| 0| 1| 0| 6| 7| 0| 3| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | 10 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Chronic Active | | 1 3.0| Ulcer | | 1 4.0| |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Chronic Active | 3 | 1 3.0| |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | 2 3 3 | 5 2.6| Basophilic Focus | X | 5 | Clear Cell Focus | X X X X X X X X X X | 16 | Eosinophilic Focus | X X X X X | 10 | Fibrosis | | 1 2.0| Hematopoietic Cell Proliferation | 1 | 1 1.0| Infarct | 4 | 2 4.0| Inflammation, Chronic Active | 1 1 1 1 1 1 1 1 2 1 1 1 1 1 1 1 1 2 1| 33 1.2| Mineralization | | 1 1.0| Mixed Cell Focus | X X X X X X | 13 | Necrosis | 2 2 2 | 5 2.2| Pigmentation | 1 | 3 1.0| Vacuolization Cytoplasmic | 2 2 3 2 2 2 2 2 2 2 1 2 2 2 1 | 23 1.9| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 82 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 4| 7| 5| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 9| 3| 6| 3| | | 1| 2| 2| 0| 1| 0| 2| 0| 2| 1| 2| 2| 0| 2| 0| 2| 2| 0| 1| 0| 6| 7| 0| 3| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | 10 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | |__________________________________________________________________________|____________| Mesentery | + + + + | 8 | Artery, Inflammation, Chronic Active | 3 | 1 3.0| Artery, Necrosis, Fibrinoid | 3 | 1 3.0| Fat, Mineralization | 1 | 2 2.0| Fat, Necrosis | 4 4 | 4 4.0| |__________________________________________________________________________|____________| Oral Mucosa | + + | 4 | Gingival, Foreign Body | X | 3 | Gingival, Inflammation, Suppurative | 2 3 | 4 2.3| |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | | 1 2.0| |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 1 1 1 1 2| 36 1.1| Duct, Cyst | | 1 2.0| |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperkeratosis, Diffuse | 2 | 3 2.0| Hyperkeratosis, Focal | 2 | 4 1.5| Inflammation, Chronic Active | 1 | 1 1.0| Inflammation, Chronic Active, | | | Multifocal | 1 | 1 1.0| Ulcer | 2 | 1 2.0| Ulcer, Multifocal | 2 | 1 2.0| Epithelium, Hyperplasia, Focal | 3 2 4 2 | 8 2.0| |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Mineralization | | 1 2.0| Glands, Ectasia | 1 1 1 1 1| 11 1.0| |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 83 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 4| 7| 5| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 9| 3| 6| 3| | | 1| 2| 2| 0| 1| 0| 2| 0| 2| 1| 2| 2| 0| 2| 0| 2| 2| 0| 1| 0| 6| 7| 0| 3| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | 10 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | Tooth | + + + +| 4 | Inflammation, Suppurative | 3 | 1 3.0| Malformation | X X X| 3 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Aorta, Mineralization | | 1 2.0| |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Chronic Active | 2 | 2 1.5| Inflammation, Suppurative | | 1 2.0| Valve, Inflammation, Chronic Active | | 1 1.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hypertrophy | 2 2 2 2 2 2 1 1 2 1 | 19 1.5| Subcapsular, Hyperplasia | 2 2 2 2 2 1 1 2 1 2 2 2 1 2 1 2 2 1 1 2 2| 40 1.5| |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | 2 | 1 2.0| |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Parathyroid Gland | + + + + M M + + + + + + M + M + M + M + M + + M M| 34 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + M| 47 | Pars Distalis, Cyst | 1 | 3 1.3| |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Follicle, Cyst | 2 2 2 | 5 2.0| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 84 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 4| 7| 5| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 9| 3| 6| 3| | | 1| 2| 2| 0| 1| 0| 2| 0| 2| 1| 2| 2| 0| 2| 0| 2| 2| 0| 1| 0| 6| 7| 0| 3| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | 10 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Coagulating Gland | | 2 | |__________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1 1 2 1 1 1 1 1 1| 38 1.0| Inflammation, Chronic Active | 1 | 1 1.0| Inflammation, Suppurative | | 1 2.0| |__________________________________________________________________________|____________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia, Basal Cell | | 1 2.0| Infiltration Cellular, Mononuclear | | | Cell | 2 2 1 1| 11 1.4| Inflammation, Chronic Active | 1 2 2 | 8 1.9| Bilateral, Duct, Ectasia | | 2 2.0| Duct, Ectasia | 2 2 3 2 2 2 2 2 3 3 2 2 | 20 2.2| |__________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1 1| 29 1.0| Mineralization | 1 | 1 1.0| |__________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Suppurative | | 1 3.0| Mineralization | 2 | 2 1.5| Germinal Epithelium, Degeneration | 2 | 3 2.0| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | | 2 3.0| Atrophy | | 1 2.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 85 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 4| 7| 5| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 9| 3| 6| 3| | | 1| 2| 2| 0| 1| 0| 2| 0| 2| 1| 2| 2| 0| 2| 0| 2| 2| 0| 1| 0| 6| 7| 0| 3| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | 10 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | Inflammation, Suppurative | | 1 2.0| Myeloid Cell, Hyperplasia | 4 4 4 | 4 4.0| |__________________________________________________________________________|____________| Lymph Node | + + + | 6 | Mediastinal, Hyperplasia, Lymphoid | | 1 3.0| |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + M + + + + + + + + + + + + + + + + + +| 47 | Hyperplasia, Lymphoid | 2 2 | 5 2.0| |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + M + + + + + + + + + + + + + + + + + + + + + +| 49 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hematopoietic Cell Proliferation | 4 2 3 4 2 | 16 2.8| |__________________________________________________________________________|____________| Thymus | + + + + + + + M + + + + + + M M + + + + + + + + +| 44 | Atrophy | 4 4 3 4 | 7 3.6| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M M M M M + M M M M M| 1 | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | | 1 1.0| Inflammation, Chronic Active | | 1 3.0| Ulcer | | 1 4.0| Subcutaneous Tissue, Inflammation, | | | Suppurative | | 1 2.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Skeletal Muscle | + | 2 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 86 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 4| 7| 5| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 9| 3| 6| 3| | | 1| 2| 2| 0| 1| 0| 2| 0| 2| 1| 2| 2| 0| 2| 0| 2| 2| 0| 1| 0| 6| 7| 0| 3| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | 10 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Cyst Epithelial Inclusion | X | 1 | |__________________________________________________________________________|____________| Peripheral Nerve | | 1 | |__________________________________________________________________________|____________| Spinal Cord | | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Erythrophagocytosis | 3 | 2 2.5| Inflammation, Chronic Active | | 3 2.0| Mineralization | 1 | 1 1.0| Pigmentation | 1 | 2 1.0| Alveolar Epithelium, Hyperplasia | 2 2 | 5 2.4| Alveolus, Infiltration Cellular, | | | Histiocyte | 3 1 | 4 2.3| Artery, Mediastinum, Mineralization | | 1 3.0| |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Foreign Body | | 1 | Inflammation, Suppurative | | 1 2.0| Nasolacrimal Duct, Inflammation, | | | Suppurative | 2 | 4 2.0| |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + + + | 6 | Atrophy | 4 2 2 | 4 3.0| Synechia | 2 2 | 2 2.0| Cornea, Inflammation, Chronic Active | 2 2 2 | 3 2.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 87 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 4| 7| 5| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 9| 3| 6| 3| | | 1| 2| 2| 0| 1| 0| 2| 0| 2| 1| 2| 2| 0| 2| 0| 2| 2| 0| 1| 0| 6| 7| 0| 3| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | 10 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| SPECIAL SENSES SYSTEM - cont | | | Cornea, Sclera, Inflammation, | | | Chronic Active | 4 | 1 4.0| Lens, Cataract | 4 2 | 3 2.7| Retina, Atrophy | 3 | 1 3.0| |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | 3 4 2| 7 3.4| Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 26 1.0| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Accumulation, Hyaline Droplet | 3 | 1 3.0| Infarct | 2 | 3 2.0| Inflammation, Chronic Active | 2 1 | 3 1.7| Metaplasia, Osseous | 2 1 | 5 1.8| Mineralization | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 32 1.0| Nephropathy | 1 1 1 1 1 1 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 42 1.2| Cortex, Cyst | 3 2 2 2 2 2 | 13 1.9| Glomerulus, Thrombosis | 1 | 1 1.0| Renal Tubule, Casts Protein | | 1 2.0| Renal Tubule, Hyperplasia | 2 2 2 | 4 1.8| |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 88 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 6| 5| 5| 7| 7| 7| 7| 6| 7| 6| 5| 7| 7| 7| 7| 2| 5| 7| 2| 4| 4| 7| 6| 5| 6| DAY ON TEST | 7| 5| 8| 2| 2| 1| 3| 0| 3| 9| 5| 3| 3| 3| 3| 3| 5| 3| 8| 7| 1| 3| 7| 4| 4| | 3| 4| 8| 4| 8| 6| 2| 3| 1| 5| 4| 1| 2| 1| 1| 9| 4| 0| 3| 7| 0| 2| 9| 7| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 20 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + M + + + + + + + + + +| Cyst | 2 | |__________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 2 | Basophilic Focus | X X | Clear Cell Focus | X X | Eosinophilic Focus | X X X | Fibrosis | 2 | Hematopoietic Cell Proliferation | | Inflammation, Chronic Active | 1 1 1 1 1 1 1 1 1 | Mixed Cell Focus | X X | Vacuolization Cytoplasmic | 2 1 1 2 2 2 1 1 2 1 | |__________________________________________________________________________| Mesentery | + | Fat, Necrosis | | |__________________________________________________________________________| Oral Mucosa | + | Gingival, Inflammation, Suppurative | 2 | |__________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Acinus, Atrophy | 1 | |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 89 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 6| 5| 5| 7| 7| 7| 7| 6| 7| 6| 5| 7| 7| 7| 7| 2| 5| 7| 2| 4| 4| 7| 6| 5| 6| DAY ON TEST | 7| 5| 8| 2| 2| 1| 3| 0| 3| 9| 5| 3| 3| 3| 3| 3| 5| 3| 8| 7| 1| 3| 7| 4| 4| | 3| 4| 8| 4| 8| 6| 2| 3| 1| 5| 4| 1| 2| 1| 1| 9| 4| 0| 3| 7| 0| 2| 9| 7| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 20 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 1 1 1 1 1 1 1 1 1 1 1| |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperkeratosis, Diffuse | 2 2 1 | Hyperkeratosis, Focal | 2 3 2 | Hyperplasia | 3 | Inflammation, Chronic Active, Focal | | Ulcer | | Epithelium, Hyperplasia, Focal | 1 3 1 2 | |__________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | 3 | Glands, Ectasia | 1 1 | |__________________________________________________________________________| Tongue | +| |__________________________________________________________________________| Tooth | + + + | Malformation | X X | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | 1 | Mineralization | 2 | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Hypertrophy | 1 1 | Subcapsular, Hyperplasia | 1 1 2 1 1 1 1 1 1 1 1 2 2 1 2 1 2 1 1 1 1 1| |__________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 90 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 6| 5| 5| 7| 7| 7| 7| 6| 7| 6| 5| 7| 7| 7| 7| 2| 5| 7| 2| 4| 4| 7| 6| 5| 6| DAY ON TEST | 7| 5| 8| 2| 2| 1| 3| 0| 3| 9| 5| 3| 3| 3| 3| 3| 5| 3| 8| 7| 1| 3| 7| 4| 4| | 3| 4| 8| 4| 8| 6| 2| 3| 1| 5| 4| 1| 2| 1| 1| 9| 4| 0| 3| 7| 0| 2| 9| 7| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 20 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ENDOCRINE SYSTEM - cont | | |__________________________________________________________________________| Parathyroid Gland | + M M + M + + + M + M + + + + + + + + + + + + M +| Cyst | | |__________________________________________________________________________| Pituitary Gland | + + + + + M + + + + M + + + + + + + + I + + + + +| Pars Distalis, Cyst | 1 | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicle, Cyst | 2 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | |__________________________________________________________________________| Peritoneum | + | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | 3 | Infiltration Cellular, Mononuclear | | Cell | 1 1 1 1 1 2 1 1 1 1 | |__________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 1 1 2 1 1 1 | Inflammation, Chronic Active | 2 2 | Bilateral, Duct, Ectasia | 2| Duct, Ectasia | 2 2 3 3 3 2 | |__________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mononuclear | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 | Metaplasia, Mucous | | |__________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | 3 | |__________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Germinal Epithelium, Degeneration | 4 | __________________________________________|__________________________________________________________________________| Page 91 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 6| 5| 5| 7| 7| 7| 7| 6| 7| 6| 5| 7| 7| 7| 7| 2| 5| 7| 2| 4| 4| 7| 6| 5| 6| DAY ON TEST | 7| 5| 8| 2| 2| 1| 3| 0| 3| 9| 5| 3| 3| 3| 3| 3| 5| 3| 8| 7| 1| 3| 7| 4| 4| | 3| 4| 8| 4| 8| 6| 2| 3| 1| 5| 4| 1| 2| 1| 1| 9| 4| 0| 3| 7| 0| 2| 9| 7| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 20 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Myeloid Cell, Hyperplasia | | |__________________________________________________________________________| Lymph Node | + + +| Pancreatic, Hyperplasia, Lymphoid | | |__________________________________________________________________________| Lymph Node, Mandibular | + + + + + M + + + + M + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | 2 2 | |__________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + M + + + + + + + +| Angiectasis | | Atrophy | 3 | Ectasia | 4 | Hematopoietic Cell Proliferation | 1 | Inflammation, Chronic Active | 1 | |__________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Fibrosis | 2 | Hematopoietic Cell Proliferation | 4 3 3 2 4 2 3 3| Lymphoid Follicle, Depletion Cellular| 4 | |__________________________________________________________________________| Thymus | + + M + + M + + + + + + + + + + + M + + + + + + +| Atrophy | 3 4| Thymocyte, Necrosis | 3 4 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperostosis | 2 | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | __________________________________________|__________________________________________________________________________| Page 92 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 6| 5| 5| 7| 7| 7| 7| 6| 7| 6| 5| 7| 7| 7| 7| 2| 5| 7| 2| 4| 4| 7| 6| 5| 6| DAY ON TEST | 7| 5| 8| 2| 2| 1| 3| 0| 3| 9| 5| 3| 3| 3| 3| 3| 5| 3| 8| 7| 1| 3| 7| 4| 4| | 3| 4| 8| 4| 8| 6| 2| 3| 1| 5| 4| 1| 2| 1| 1| 9| 4| 0| 3| 7| 0| 2| 9| 7| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 20 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| NERVOUS SYSTEM - cont | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Granulomatous | 4| Inflammation, Suppurative | | Alveolar Epithelium, Hyperplasia | 3 2 1 | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Nasolacrimal Duct, Inflammation, | | Suppurative | 2 3 2 1 | |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | + | Atrophy | 3 | Synechia | 2 | Cornea, Inflammation, Chronic Active | 2 | Lens, Cataract | 2 | |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 2 2 | Infiltration Cellular, Mononuclear | | Cell | 1 1 1 1 1 1 1 1 | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Infarct | 2 2 2 2 | Inflammation, Chronic Active | 2 | Metaplasia, Osseous | 1 2 | Mineralization | 1 1 1 1 1 1 1 1 | Nephropathy | 2 2 1 1 4 1 1 2 1 1 1 1 | Cortex, Cyst | 2 1 1 | __________________________________________|__________________________________________________________________________| Page 93 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 _____________________________________________________________________________________________________________________ | 6| 5| 5| 7| 7| 7| 7| 6| 7| 6| 5| 7| 7| 7| 7| 2| 5| 7| 2| 4| 4| 7| 6| 5| 6| DAY ON TEST | 7| 5| 8| 2| 2| 1| 3| 0| 3| 9| 5| 3| 3| 3| 3| 3| 5| 3| 8| 7| 1| 3| 7| 4| 4| | 3| 4| 8| 4| 8| 6| 2| 3| 1| 5| 4| 1| 2| 1| 1| 9| 4| 0| 3| 7| 0| 2| 9| 7| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 20 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| URINARY SYSTEM - cont | | |__________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________ Page 94 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 2| 4| 5| 5| 5| 6| 7| 7| 7| 5| 7| 3| 7| 1| 2| 5| 7| 7| 7| 5| 1| 5| 7| 4| 1| | DAY ON TEST | 1| 9| 2| 5| 4| 5| 0| 1| 2| 0| 3| 3| 3| 1| 7| 0| 3| 3| 3| 6| 1| 2| 3| 9| 1| | | 1| 2| 0| 3| 0| 6| 9| 5| 6| 5| 2| 7| 2| 4| 4| 2| 1| 2| 1| 9| 3| 5| 2| 4| 3| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| A | 20 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Gallbladder | + + M + + + + + + + + + + + + + + + + + + + + + +| 48 | Cyst | | 1 2.0| |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | | 1 2.0| Basophilic Focus | X X | 4 | Clear Cell Focus | X X | 4 | Eosinophilic Focus | X | 4 | Fibrosis | | 1 2.0| Hematopoietic Cell Proliferation | 3 | 1 3.0| Inflammation, Chronic Active | 1 1 | 11 1.0| Mixed Cell Focus | X | 3 | Vacuolization Cytoplasmic | 2 2 1 1 3 3 1 | 17 1.6| |__________________________________________________________________________|____________| Mesentery | + + | 3 | Fat, Necrosis | 4 4 | 2 4.0| |__________________________________________________________________________|____________| Oral Mucosa | | 1 | Gingival, Inflammation, Suppurative | | 1 2.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 95 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 2| 4| 5| 5| 5| 6| 7| 7| 7| 5| 7| 3| 7| 1| 2| 5| 7| 7| 7| 5| 1| 5| 7| 4| 1| | DAY ON TEST | 1| 9| 2| 5| 4| 5| 0| 1| 2| 0| 3| 3| 3| 1| 7| 0| 3| 3| 3| 6| 1| 2| 3| 9| 1| | | 1| 2| 0| 3| 0| 6| 9| 5| 6| 5| 2| 7| 2| 4| 4| 2| 1| 2| 1| 9| 3| 5| 2| 4| 3| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| A | 20 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Acinus, Atrophy | | 1 1.0| |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 1 1 1 1 1 1 1 1 | 23 1.0| |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperkeratosis, Diffuse | 2 2 1 | 6 1.7| Hyperkeratosis, Focal | 1 2 2 | 6 2.0| Hyperplasia | | 1 3.0| Inflammation, Chronic Active, Focal | 2 1 | 2 1.5| Ulcer | 2 | 1 2.0| Epithelium, Hyperplasia, Focal | 2 1 2 2 3 | 9 1.9| |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia, Focal | | 1 3.0| Glands, Ectasia | 1 2 | 4 1.3| |__________________________________________________________________________|____________| Tongue | + | 2 | |__________________________________________________________________________|____________| Tooth | | 3 | Malformation | | 2 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Chronic Active | | 1 1.0| Mineralization | | 1 2.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 96 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 2| 4| 5| 5| 5| 6| 7| 7| 7| 5| 7| 3| 7| 1| 2| 5| 7| 7| 7| 5| 1| 5| 7| 4| 1| | DAY ON TEST | 1| 9| 2| 5| 4| 5| 0| 1| 2| 0| 3| 3| 3| 1| 7| 0| 3| 3| 3| 6| 1| 2| 3| 9| 1| | | 1| 2| 0| 3| 0| 6| 9| 5| 6| 5| 2| 7| 2| 4| 4| 2| 1| 2| 1| 9| 3| 5| 2| 4| 3| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| A | 20 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ENDOCRINE SYSTEM - cont | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hypertrophy | | 2 1.0| Subcapsular, Hyperplasia | 1 1 1 1 2 1 1 1 2 3 2 1 2 1 | 36 1.3| |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Parathyroid Gland | M + + + + M + + + + + + + + + + M + + + + + + + +| 41 | Cyst | 1 | 1 1.0| |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 47 | Pars Distalis, Cyst | 1 | 2 1.0| |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Follicle, Cyst | | 1 2.0| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | |__________________________________________________________________________|____________| Peritoneum | | 1 | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Atrophy | | 1 3.0| Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 1 1 | 16 1.1| |__________________________________________________________________________|____________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | 2 1 1 1 1 1 1 | 13 1.2| Inflammation, Chronic Active | | 2 2.0| Bilateral, Duct, Ectasia | | 1 2.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 97 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 2| 4| 5| 5| 5| 6| 7| 7| 7| 5| 7| 3| 7| 1| 2| 5| 7| 7| 7| 5| 1| 5| 7| 4| 1| | DAY ON TEST | 1| 9| 2| 5| 4| 5| 0| 1| 2| 0| 3| 3| 3| 1| 7| 0| 3| 3| 3| 6| 1| 2| 3| 9| 1| | | 1| 2| 0| 3| 0| 6| 9| 5| 6| 5| 2| 7| 2| 4| 4| 2| 1| 2| 1| 9| 3| 5| 2| 4| 3| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| A | 20 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | Duct, Ectasia | 2 3 3 2 2 2 2 3 3 | 15 2.5| |__________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 2 1 1 1 | 22 1.0| Metaplasia, Mucous | 3 | 1 3.0| |__________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Atrophy | | 1 3.0| |__________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Germinal Epithelium, Degeneration | | 1 4.0| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Myeloid Cell, Hyperplasia | 4 4 | 2 4.0| |__________________________________________________________________________|____________| Lymph Node | + + | 5 | Pancreatic, Hyperplasia, Lymphoid | 3 | 1 3.0| |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + I + + +| 47 | Hyperplasia, Lymphoid | 3 2 | 4 2.3| |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + M + + + + + + + + + + + + + + + + +| 48 | Angiectasis | 3 | 1 3.0| Atrophy | | 1 3.0| Ectasia | 2 | 2 3.0| Hematopoietic Cell Proliferation | 2 | 2 1.5| Inflammation, Chronic Active | | 1 1.0| |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Fibrosis | | 1 2.0| Hematopoietic Cell Proliferation | 4 2 3 3 | 12 3.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 98 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 2| 4| 5| 5| 5| 6| 7| 7| 7| 5| 7| 3| 7| 1| 2| 5| 7| 7| 7| 5| 1| 5| 7| 4| 1| | DAY ON TEST | 1| 9| 2| 5| 4| 5| 0| 1| 2| 0| 3| 3| 3| 1| 7| 0| 3| 3| 3| 6| 1| 2| 3| 9| 1| | | 1| 2| 0| 3| 0| 6| 9| 5| 6| 5| 2| 7| 2| 4| 4| 2| 1| 2| 1| 9| 3| 5| 2| 4| 3| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| A | 20 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | Lymphoid Follicle, Depletion Cellular| | 1 4.0| |__________________________________________________________________________|____________| Thymus | + M + + + + + + + + + + + + + + + + + + + + + + +| 46 | Atrophy | 4 2 | 4 3.3| Thymocyte, Necrosis | 3 | 3 3.3| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperostosis | | 1 2.0| _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Granulomatous | | 1 4.0| Inflammation, Suppurative | 2 | 1 2.0| Alveolar Epithelium, Hyperplasia | 2 1 1 | 6 1.7| |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Nasolacrimal Duct, Inflammation, | | | Suppurative | 2 2 3 | 7 2.1| |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 99 NTP Experiment-Test: 50215-03 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00 Route: GAVAGE Time: 12:43:35 __________________________________________________________________________________________________________________________________ | 2| 4| 5| 5| 5| 6| 7| 7| 7| 5| 7| 3| 7| 1| 2| 5| 7| 7| 7| 5| 1| 5| 7| 4| 1| | DAY ON TEST | 1| 9| 2| 5| 4| 5| 0| 1| 2| 0| 3| 3| 3| 1| 7| 0| 3| 3| 3| 6| 1| 2| 3| 9| 1| | | 1| 2| 0| 3| 0| 6| 9| 5| 6| 5| 2| 7| 2| 4| 4| 2| 1| 2| 1| 9| 3| 5| 2| 4| 3| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| A | 20 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + | 3 | Atrophy | 4 | 2 3.5| Synechia | 2 | 2 2.0| Cornea, Inflammation, Chronic Active | 2 2 | 3 2.0| Lens, Cataract | 2 | 2 2.0| |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | 3 | 4 2.3| Infiltration Cellular, Mononuclear | | | Cell | 1 1 1 1 1 1 | 14 1.0| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infarct | 2 | 5 2.0| Inflammation, Chronic Active | 1 | 2 1.5| Metaplasia, Osseous | 1 | 3 1.3| Mineralization | 1 1 1 1 1 1 1 1 1 1 | 18 1.0| Nephropathy | 1 2 1 1 1 1 1 | 19 1.4| Cortex, Cyst | 2 | 4 1.5| |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 100 ------------------------------------------------------------ ---------- END OF REPORT ---------- ------------------------------------------------------------