TDMS Study 88036-03 Pathology Tables
NTP Experiment-Test: 88036-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 02/25/99
Route: DOSED FEED Time: 11:42:20
2 YEAR CHRONIC RATS
Facility: Battelle Columbus Laboratory
Chemical CAS #: 84-65-1
Lock Date: 07/23/97
Cage Range: All
Reasons For Removal: 25019 Moribund Sacrifice 25020 Natural Death
25021 Terminal Sacrifice
Removal Date Range: All
Treatment Groups: Include All
a Number of animals examined microscopically at site and number of animals with lesion
Page 1
NTP Experiment-Test: 88036-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 02/25/99
Route: DOSED FEED Time: 11:42:20
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS FEMALE 0 PPM 469 PPM 938 PPM 1875 PPM 3750 PPM
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 60 50 50 50 60
Early Deaths
Moribund Sacrifice 14 7 12 7 6
Natural Death 13 3 3 6 4
Survivors
Terminal Sacrifice 23 40 35 37 40
Animals Examined Microscopically 50 50 50 50 50
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Intestine Large, Colon (50) (50) (49) (50) (49)
Parasite Metazoan 2 (4%) 2 (4%) 1 (2%)
Intestine Large, Rectum (50) (50) (49) (50) (50)
Inflammation 1 (2%)
Parasite Metazoan 6 (12%) 11 (22%) 2 (4%) 8 (16%)
Intestine Large, Cecum (50) (49) (49) (50) (49)
Inflammation 1 (2%)
Intestine Small, Jejunum (50) (50) (50) (50) (49)
Inflammation 1 (2%)
Intestine Small, Ileum (50) (49) (49) (50) (49)
Cyst 1 (2%)
Inflammation 1 (2%)
Liver (50) (50) (50) (50) (49)
Angiectasis 3 (6%) 15 (30%) 18 (36%) 15 (30%) 21 (43%)
Basophilic Focus 37 (74%) 50 (100%) 34 (68%) 33 (66%) 15 (31%)
Clear Cell Focus 1 (2%) 3 (6%) 2 (4%) 6 (12%) 3 (6%)
Degeneration, Cystic 5 (10%) 10 (20%) 10 (20%) 6 (12%)
Eosinophilic Focus 8 (16%) 32 (64%) 34 (68%) 39 (78%) 34 (69%)
Fatty Change 1 (2%)
Fibrosis 1 (2%)
Hematopoietic Cell Proliferation 3 (6%) 1 (2%)
Hepatodiaphragmatic Nodule 5 (10%) 10 (20%) 7 (14%) 6 (12%) 5 (10%)
Inflammation 25 (50%) 46 (92%) 44 (88%) 38 (76%) 46 (94%)
Mixed Cell Focus 3 (6%) 30 (60%) 20 (40%) 23 (46%) 13 (27%)
Necrosis 4 (8%) 4 (8%) 5 (10%) 6 (12%) 3 (6%)
Thrombosis 1 (2%) 1 (2%)
Vacuolization Cytoplasmic 5 (10%) 4 (8%) 6 (12%) 4 (8%) 4 (8%)
Bile Duct, Cholangiofibrosis 1 (2%) 1 (2%)
Bile Duct, Cyst 1 (2%) 1 (2%) 2 (4%)
Bile Duct, Hyperplasia 17 (34%) 22 (44%) 20 (40%) 20 (40%) 12 (24%)
Centrilobular, Degeneration 1 (2%) 1 (2%) 1 (2%) 1 (2%)
Centrilobular, Hypertrophy 18 (36%) 23 (46%) 19 (38%) 26 (53%)
Centrilobular, Necrosis 1 (2%)
Mesentery (4) (4) (6) (3) (2)
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 02/25/99
Route: DOSED FEED Time: 11:42:20
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS FEMALE 0 PPM 469 PPM 938 PPM 1875 PPM 3750 PPM
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Inflammation 1 (17%)
Fat, Necrosis 3 (75%) 4 (100%) 4 (67%) 2 (67%) 2 (100%)
Oral Mucosa (1) (1)
Gingival, Inflammation 1 (100%)
Pancreas (50) (50) (50) (50) (49)
Atrophy 21 (42%) 27 (54%) 16 (32%) 22 (44%) 9 (18%)
Hyperplasia 2 (4%) 1 (2%) 2 (4%) 1 (2%)
Hypertrophy, Focal 1 (2%) 2 (4%) 1 (2%)
Metaplasia, Hepatocyte 1 (2%) 1 (2%)
Pigmentation 1 (2%)
Artery, Inflammation 1 (2%)
Duct, Cyst 1 (2%)
Salivary Glands (50) (50) (50) (50) (48)
Atrophy 1 (2%) 1 (2%)
Fibrosis 1 (2%)
Inflammation 1 (2%)
Stomach, Forestomach (50) (50) (50) (50) (49)
Edema 1 (2%)
Erosion 1 (2%)
Foreign Body 1 (2%)
Hyperplasia 4 (8%) 1 (2%) 2 (4%) 2 (4%) 2 (4%)
Inflammation 1 (2%) 1 (2%) 2 (4%) 1 (2%)
Necrosis 1 (2%)
Ulcer 6 (12%) 1 (2%) 3 (6%) 1 (2%)
Stomach, Glandular (50) (50) (50) (50) (49)
Edema 1 (2%)
Erosion 2 (4%) 1 (2%) 1 (2%) 1 (2%)
Inflammation 1 (2%)
Mineralization 1 (2%)
Ulcer 2 (4%) 1 (2%)
Tooth (1)
Inflammation 1 (100%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Blood Vessel (50) (50) (50) (50) (49)
Inflammation 1 (2%) 1 (2%)
Heart (50) (50) (50) (50) (48)
Cardiomyopathy 22 (44%) 25 (50%) 27 (54%) 28 (56%) 28 (58%)
Inflammation 2 (4%) 9 (18%) 4 (8%) 1 (2%) 3 (6%)
Artery, Inflammation 2 (4%)
Atrium, Thrombosis 2 (4%) 1 (2%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 02/25/99
Route: DOSED FEED Time: 11:42:20
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS FEMALE 0 PPM 469 PPM 938 PPM 1875 PPM 3750 PPM
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (50) (50) (50) (50) (49)
Degeneration, Cystic 6 (12%) 2 (4%) 6 (12%) 9 (18%) 3 (6%)
Hematopoietic Cell Proliferation 1 (2%)
Hemorrhage 1 (2%)
Hyperplasia 13 (26%) 12 (24%) 17 (34%) 17 (34%) 3 (6%)
Hypertrophy 3 (6%) 1 (2%) 1 (2%) 7 (14%) 5 (10%)
Mineralization 1 (2%)
Necrosis 1 (2%)
Vacuolization Cytoplasmic 1 (2%) 2 (4%) 1 (2%) 4 (8%) 3 (6%)
Adrenal Medulla (50) (50) (50) (50) (49)
Cyst 1 (2%)
Hyperplasia 8 (16%) 3 (6%) 4 (8%) 4 (8%) 6 (12%)
Islets, Pancreatic (49) (50) (50) (50) (49)
Hyperplasia 1 (2%)
Parathyroid Gland (42) (44) (48) (48) (41)
Hyperplasia 1 (2%) 1 (2%)
Hyperplasia, Focal 1 (2%)
Pituitary Gland (50) (50) (50) (49) (49)
Angiectasis 1 (2%) 12 (24%) 2 (4%) 10 (20%) 6 (12%)
Cyst 9 (18%) 7 (14%) 3 (6%) 3 (6%)
Degeneration 1 (2%) 1 (2%)
Pars Distalis, Hyperplasia 24 (48%) 27 (54%) 21 (42%) 26 (53%) 32 (65%)
Pars Intermedia, Hyperplasia 1 (2%) 1 (2%)
Pars Nervosa, Hyperplasia 1 (2%)
Thyroid Gland (50) (50) (50) (50) (49)
C-Cell, Hyperplasia 21 (42%) 29 (58%) 20 (40%) 18 (36%) 18 (37%)
Follicle, Cyst 1 (2%)
Follicular Cell, Hyperplasia 1 (2%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Clitoral Gland (49) (46) (48) (49) (48)
Hyperplasia 2 (4%) 5 (11%) 8 (17%) 4 (8%) 3 (6%)
Inflammation 10 (20%) 21 (46%) 13 (27%) 11 (22%) 12 (25%)
Duct, Cyst 4 (8%) 5 (11%) 2 (4%) 4 (8%) 3 (6%)
Ovary (50) (50) (50) (50) (49)
Atrophy 1 (2%)
Cyst 5 (10%) 8 (16%) 9 (18%) 8 (16%) 6 (12%)
Granulosa Cell, Hyperplasia 1 (2%) 1 (2%)
Interstitial Cell, Hyperplasia 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 02/25/99
Route: DOSED FEED Time: 11:42:20
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS FEMALE 0 PPM 469 PPM 938 PPM 1875 PPM 3750 PPM
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM - CONT
Oviduct (1) (1)
Inflammation 1 (100%)
Uterus (50) (50) (50) (50) (49)
Angiectasis 1 (2%)
Hemorrhage 1 (2%) 1 (2%)
Hyperplasia 3 (6%) 3 (6%) 3 (6%) 4 (8%)
Inflammation 1 (2%)
Cervix, Hypertrophy 1 (2%)
Cervix, Inflammation 1 (2%) 1 (2%)
Vagina (1) (1)
Cyst, Squamous 1 (100%)
Inflammation, Suppurative 1 (100%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (50) (50) (50) (50) (50)
Atrophy 4 (8%) 13 (26%) 13 (26%) 11 (22%) 13 (26%)
Hyperplasia 19 (38%) 31 (62%) 28 (56%) 19 (38%) 23 (46%)
Infiltration Cellular, Histiocyte 1 (2%)
Inflammation 1 (2%)
Lymph Node, Mandibular (49) (49) (50) (48) (47)
Ectasia 2 (4%) 2 (4%) 1 (2%)
Hyperplasia, Plasma Cell 1 (2%) 1 (2%) 1 (2%)
Inflammation 1 (2%) 1 (2%)
Lymph Node, Mesenteric (50) (49) (49) (50) (49)
Atrophy 1 (2%) 1 (2%) 1 (2%)
Ectasia 1 (2%) 1 (2%) 1 (2%)
Hyperplasia, Plasma Cell 1 (2%)
Spleen (50) (50) (50) (50) (49)
Congestion 1 (2%) 46 (92%) 42 (84%) 44 (88%) 45 (92%)
Fibrosis 1 (2%)
Hematopoietic Cell Proliferation 39 (78%) 50 (100%) 47 (94%) 47 (94%) 46 (94%)
Infarct 1 (2%) 1 (2%)
Pigmentation 33 (66%) 45 (90%) 48 (96%) 48 (96%) 47 (96%)
Capsule, Fibrosis 2 (4%) 1 (2%) 1 (2%)
Lymphoid Follicle, Atrophy 1 (2%) 2 (4%) 3 (6%)
Red Pulp, Depletion Cellular 1 (2%) 2 (4%) 2 (4%)
Thymus (46) (48) (48) (49) (46)
Atrophy 44 (96%) 48 (100%) 47 (98%) 49 (100%) 46 (100%)
Artery, Inflammation 1 (2%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 02/25/99
Route: DOSED FEED Time: 11:42:20
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS FEMALE 0 PPM 469 PPM 938 PPM 1875 PPM 3750 PPM
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Mammary Gland (50) (50) (50) (50) (50)
Cyst 5 (10%) 3 (6%) 4 (8%) 2 (4%) 1 (2%)
Hyperplasia 23 (46%) 8 (16%) 11 (22%) 5 (10%) 5 (10%)
Inflammation 1 (2%)
Skin (50) (50) (50) (50) (50)
Acanthosis 1 (2%)
Cyst Epithelial Inclusion 1 (2%)
Ulcer 1 (2%)
Hair Follicle, Atrophy 1 (2%)
Subcutaneous Tissue, Necrosis 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (50) (50) (50) (50) (50)
Fibrous Osteodystrophy 1 (2%)
Osteopetrosis 3 (6%) 2 (4%) 3 (6%) 1 (2%)
Osteoporosis 1 (2%)
Maxilla, Cyst 1 (2%)
Maxilla, Inflammation 1 (2%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (50) (50) (50) (50) (49)
Gliosis 1 (2%)
Hemorrhage 1 (2%) 1 (2%) 1 (2%)
Inflammation 1 (2%) 1 (2%) 1 (2%)
Mineralization 1 (2%)
Thrombosis 1 (2%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (50) (50) (50) (50) (48)
Foreign Body 1 (2%)
Inflammation 13 (26%) 18 (36%) 19 (38%) 21 (42%) 24 (50%)
Pigmentation 44 (88%) 50 (100%) 48 (96%) 47 (94%) 48 (100%)
Thrombosis 1 (2%)
Alveolar Epithelium, Hyperplasia 3 (6%) 7 (14%) 2 (4%) 5 (10%)
Nose (50) (50) (50) (50) (50)
Foreign Body 4 (8%) 1 (2%) 1 (2%) 1 (2%)
Inflammation 4 (8%) 2 (4%) 1 (2%) 1 (2%) 1 (2%)
Nasolacrimal Duct, Inflammation 1 (2%)
Respiratory Epithelium, Inflammation 1 (2%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 02/25/99
Route: DOSED FEED Time: 11:42:20
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS FEMALE 0 PPM 469 PPM 938 PPM 1875 PPM 3750 PPM
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Eye (2) (3) (2) (3) (5)
Cataract 2 (100%) 3 (100%) 1 (50%) 2 (67%) 3 (60%)
Degeneration 1 (50%) 2 (67%) 2 (40%)
Hemorrhage 1 (20%)
Inflammation 1 (50%)
Cornea, Inflammation 1 (50%)
Lens, Mineralization 2 (67%) 1 (50%)
Retina, Degeneration 1 (50%) 3 (100%) 2 (100%) 1 (33%) 3 (60%)
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (50) (50) (50) (50) (49)
Accumulation, Hyaline Droplet 33 (66%) 48 (96%) 45 (90%) 44 (88%) 44 (90%)
Hydronephrosis 2 (4%) 1 (2%)
Infarct 1 (2%) 1 (2%)
Inflammation 3 (6%)
Nephropathy 39 (78%) 49 (98%) 47 (94%) 49 (98%) 49 (100%)
Pigmentation 27 (54%) 50 (100%) 48 (96%) 50 (100%) 47 (96%)
Medulla, Mineralization 17 (34%) 25 (50%) 27 (54%) 28 (56%) 20 (41%)
Pelvis, Calculus Micro Observation Only 1 (2%)
Pelvis, Inflammation 1 (2%) 1 (2%)
Renal Tubule, Hyperplasia 12 (24%) 13 (26%) 15 (30%) 11 (22%)
Renal Tubule, Hyperplasia, Oncocytic 2 (4%) 1 (2%) 1 (2%)
Transitional Epithelium, Hyperplasia 5 (10%) 12 (24%) 3 (6%) 10 (20%)
Urinary Bladder (49) (49) (49) (50) (49)
Calculus Micro Observation Only 1 (2%)
Inflammation 13 (27%) 16 (33%) 9 (18%) 25 (50%) 17 (35%)
Transitional Epithelium, Hyperplasia 1 (2%) 1 (2%) 4 (8%) 4 (8%)
Transitional Epithelium, Metaplasia, Squamous 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 02/25/99
Route: DOSED FEED Time: 11:42:20
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS MALE 0 PPM 469 PPM 938 PPM 1875 PPM 3750 PPM
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 60 50 50 50 60
Early Deaths
Moribund Sacrifice 21 21 16 14 17
Natural Death 7 6 12 10 11
Survivors
Terminal Sacrifice 21 23 22 26 22
Natural Death 1
Animals Examined Microscopically 50 50 50 50 50
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Esophagus (50) (50) (50) (50) (49)
Inflammation 1 (2%)
Ulcer 1 (2%) 1 (2%)
Intestine Large, Colon (50) (50) (50) (50) (50)
Edema 1 (2%)
Inflammation 1 (2%)
Mineralization 1 (2%)
Parasite Metazoan 1 (2%) 1 (2%) 1 (2%)
Intestine Large, Rectum (50) (50) (50) (50) (50)
Mineralization 1 (2%)
Parasite Metazoan 5 (10%) 7 (14%) 7 (14%) 5 (10%) 3 (6%)
Intestine Large, Cecum (50) (50) (50) (50) (49)
Edema 1 (2%)
Inflammation 2 (4%) 2 (4%)
Mineralization 1 (2%)
Artery, Inflammation 1 (2%)
Intestine Small, Duodenum (50) (50) (50) (50) (50)
Erosion 1 (2%) 1 (2%) 1 (2%)
Inflammation 2 (4%)
Ulcer 2 (4%) 1 (2%) 1 (2%)
Intestine Small, Jejunum (50) (50) (50) (50) (50)
Inflammation 1 (2%)
Ulcer 1 (2%)
Intestine Small, Ileum (50) (50) (50) (50) (50)
Inflammation 1 (2%) 1 (2%) 1 (2%)
Liver (50) (50) (50) (50) (50)
Angiectasis 6 (12%) 21 (42%) 13 (26%) 9 (18%) 9 (18%)
Basophilic Focus 25 (50%) 35 (70%) 35 (70%) 32 (64%) 23 (46%)
Clear Cell Focus 9 (18%) 5 (10%) 6 (12%) 8 (16%) 5 (10%)
Congestion 1 (2%)
Degeneration, Cystic 9 (18%) 31 (62%) 36 (72%) 28 (56%) 29 (58%)
Eosinophilic Focus 9 (18%) 22 (44%) 30 (60%) 29 (58%) 20 (40%)
Hematopoietic Cell Proliferation 2 (4%) 1 (2%) 3 (6%) 3 (6%)
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 02/25/99
Route: DOSED FEED Time: 11:42:20
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS MALE 0 PPM 469 PPM 938 PPM 1875 PPM 3750 PPM
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Hemorrhage 1 (2%)
Hepatodiaphragmatic Nodule 5 (10%) 9 (18%) 2 (4%) 5 (10%) 4 (8%)
Inflammation 13 (26%) 30 (60%) 28 (56%) 30 (60%) 27 (54%)
Mineralization 1 (2%)
Mixed Cell Focus 4 (8%) 12 (24%) 15 (30%) 13 (26%) 10 (20%)
Necrosis 5 (10%) 5 (10%) 5 (10%) 7 (14%) 5 (10%)
Pigmentation 1 (2%)
Thrombosis 1 (2%) 1 (2%) 1 (2%)
Vacuolization Cytoplasmic 5 (10%) 18 (36%) 23 (46%) 17 (34%) 23 (46%)
Bile Duct, Cyst 1 (2%) 1 (2%)
Bile Duct, Hyperplasia 48 (96%) 50 (100%) 47 (94%) 49 (98%) 47 (94%)
Centrilobular, Degeneration 2 (4%) 2 (4%) 2 (4%) 1 (2%) 2 (4%)
Centrilobular, Hypertrophy 4 (8%) 21 (42%) 13 (26%) 29 (58%)
Centrilobular, Necrosis 1 (2%) 2 (4%) 1 (2%)
Mesentery (10) (4) (6) (7) (6)
Inflammation 2 (20%)
Artery, Inflammation 1 (14%)
Artery, Mineralization 2 (33%)
Fat, Inflammation 1 (14%)
Fat, Necrosis 6 (60%) 3 (75%) 2 (33%) 2 (29%) 4 (67%)
Oral Mucosa (1) (2) (1)
Pharyngeal, Hyperplasia 1 (50%)
Pancreas (50) (50) (50) (50) (50)
Atrophy 26 (52%) 30 (60%) 25 (50%) 28 (56%) 24 (48%)
Hyperplasia 2 (4%) 4 (8%) 2 (4%) 2 (4%) 1 (2%)
Hypertrophy, Focal 1 (2%) 6 (12%) 3 (6%) 2 (4%) 2 (4%)
Inflammation 2 (4%) 2 (4%) 1 (2%)
Artery, Hypertrophy 1 (2%)
Artery, Inflammation 1 (2%) 1 (2%) 3 (6%) 1 (2%) 5 (10%)
Artery, Mineralization 1 (2%) 3 (6%)
Duct, Cyst 1 (2%)
Duct, Hyperplasia 1 (2%)
Salivary Glands (50) (50) (48) (50) (47)
Atrophy 1 (2%) 3 (6%) 1 (2%)
Hyperplasia 1 (2%)
Inflammation 1 (2%)
Mineralization 1 (2%)
Stomach, Forestomach (50) (50) (50) (50) (50)
Edema 2 (4%) 4 (8%) 2 (4%) 2 (4%) 3 (6%)
Foreign Body 1 (2%)
Hyperplasia 4 (8%) 11 (22%) 7 (14%) 4 (8%) 4 (8%)
Inflammation 4 (8%) 2 (4%) 1 (2%)
Mineralization 2 (4%) 4 (8%)
Perforation 1 (2%) 1 (2%)
Ulcer 3 (6%) 8 (16%) 4 (8%) 3 (6%)
Stomach, Glandular (50) (50) (50) (50) (50)
Erosion 2 (4%) 4 (8%) 1 (2%) 2 (4%) 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 02/25/99
Route: DOSED FEED Time: 11:42:20
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS MALE 0 PPM 469 PPM 938 PPM 1875 PPM 3750 PPM
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Fibrosis 1 (2%) 1 (2%)
Hyperplasia 1 (2%)
Inflammation 1 (2%) 1 (2%) 1 (2%) 1 (2%)
Mineralization 2 (4%) 4 (8%) 7 (14%) 10 (20%) 10 (20%)
Ulcer 3 (6%) 2 (4%) 3 (6%) 1 (2%) 3 (6%)
Tooth (1)
Inflammation 1 (100%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Blood Vessel (50) (50) (50) (50) (50)
Mineralization 3 (6%) 3 (6%) 4 (8%) 7 (14%) 7 (14%)
Aorta, Aneurysm 1 (2%)
Heart (50) (50) (50) (50) (50)
Angiectasis 1 (2%)
Cardiomyopathy 42 (84%) 44 (88%) 44 (88%) 43 (86%) 41 (82%)
Inflammation 1 (2%) 1 (2%) 2 (4%) 1 (2%)
Mineralization 1 (2%) 2 (4%) 4 (8%) 5 (10%) 5 (10%)
Artery, Inflammation 1 (2%) 1 (2%)
Atrium, Thrombosis 6 (12%) 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (50) (50) (50) (50) (50)
Accessory Adrenal Cortical Nodule 1 (2%)
Degeneration, Cystic 7 (14%) 4 (8%) 3 (6%) 7 (14%) 9 (18%)
Hematopoietic Cell Proliferation 1 (2%)
Hemorrhage 1 (2%) 2 (4%)
Hyperplasia 8 (16%) 10 (20%) 8 (16%) 13 (26%) 6 (12%)
Hypertrophy 4 (8%) 1 (2%) 2 (4%) 4 (8%) 1 (2%)
Necrosis 1 (2%)
Pigmentation 1 (2%)
Thrombosis 1 (2%) 1 (2%)
Vacuolization Cytoplasmic 3 (6%) 2 (4%) 1 (2%) 4 (8%)
Adrenal Medulla (50) (50) (50) (50) (50)
Angiectasis 1 (2%)
Hyperplasia 17 (34%) 24 (48%) 22 (44%) 20 (40%) 15 (30%)
Thrombosis 1 (2%)
Parathyroid Gland (49) (48) (48) (50) (45)
Hyperplasia 5 (10%) 13 (27%) 19 (40%) 20 (40%) 12 (27%)
Hyperplasia, Focal 1 (2%) 1 (2%) 1 (2%) 1 (2%)
Inflammation 1 (2%)
Pituitary Gland (50) (50) (49) (50) (50)
Angiectasis 1 (2%) 1 (2%) 1 (2%) 1 (2%)
Cyst 2 (4%) 3 (6%) 3 (6%) 3 (6%) 6 (12%)
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 02/25/99
Route: DOSED FEED Time: 11:42:20
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS MALE 0 PPM 469 PPM 938 PPM 1875 PPM 3750 PPM
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM - CONT
Hemorrhage 1 (2%) 1 (2%)
Thrombosis 1 (2%)
Pars Distalis, Hyperplasia 13 (26%) 26 (52%) 24 (49%) 28 (56%) 19 (38%)
Pars Intermedia, Hyperplasia 1 (2%) 2 (4%) 1 (2%)
Pars Nervosa, Hyperplasia 5 (10%) 1 (2%) 4 (8%) 1 (2%)
Thyroid Gland (50) (50) (48) (50) (47)
C-Cell, Hyperplasia 14 (28%) 22 (44%) 14 (29%) 13 (26%) 12 (26%)
Follicle, Cyst 3 (6%) 2 (4%) 3 (6%) 5 (10%) 4 (9%)
Follicular Cell, Hyperplasia 3 (6%) 3 (6%) 1 (2%) 1 (2%) 3 (6%)
Follicular Cell, Hypertrophy 1 (2%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Coagulating Gland (1)
Inflammation 1 (100%)
Epididymis (50) (50) (50) (50) (50)
Granuloma Sperm 1 (2%) 3 (6%) 1 (2%)
Inflammation 1 (2%) 1 (2%)
Mineralization 1 (2%) 1 (2%) 1 (2%)
Preputial Gland (50) (49) (50) (49) (50)
Atrophy 1 (2%)
Hyperplasia 3 (6%) 4 (8%) 3 (6%) 1 (2%)
Infiltration Cellular 1 (2%)
Inflammation 42 (84%) 34 (69%) 43 (86%) 37 (76%) 43 (86%)
Duct, Ectasia 1 (2%) 4 (8%) 4 (8%) 4 (8%)
Prostate (50) (50) (50) (50) (50)
Atrophy 1 (2%)
Cyst 1 (2%) 1 (2%)
Hyperplasia 1 (2%) 1 (2%) 1 (2%)
Inflammation 33 (66%) 37 (74%) 36 (72%) 31 (62%) 34 (68%)
Metaplasia, Squamous 1 (2%)
Mineralization 1 (2%)
Seminal Vesicle (50) (50) (50) (49) (50)
Dilatation 2 (4%)
Hyperplasia 1 (2%)
Inflammation 1 (2%) 1 (2%) 1 (2%) 1 (2%)
Mineralization 1 (2%) 1 (2%) 2 (4%) 3 (6%)
Testes (50) (50) (50) (50) (50)
Atrophy 6 (12%) 10 (20%) 7 (14%) 6 (12%) 5 (10%)
Thrombosis 1 (2%)
Artery, Inflammation 1 (2%) 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 02/25/99
Route: DOSED FEED Time: 11:42:20
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS MALE 0 PPM 469 PPM 938 PPM 1875 PPM 3750 PPM
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM - CONT
Interstitial Cell, Hyperplasia 27 (54%) 27 (54%) 26 (52%) 24 (48%) 26 (52%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (50) (50) (50) (50) (50)
Hemorrhage 2 (4%) 2 (4%) 1 (2%) 1 (2%)
Hyperplasia 25 (50%) 28 (56%) 37 (74%) 36 (72%) 33 (66%)
Myelofibrosis 1 (2%) 1 (2%)
Necrosis 1 (2%)
Lymph Node (8) (3) (1) (1) (2)
Mediastinal, Ectasia 1 (100%)
Mediastinal, Hematopoietic Cell Proliferation 1 (13%)
Mediastinal, Pigmentation 1 (13%)
Renal, Hyperplasia, Plasma Cell 1 (33%)
Lymph Node, Mandibular (50) (50) (47) (50) (47)
Atrophy 1 (2%) 1 (2%)
Ectasia 8 (16%) 2 (4%) 2 (4%) 1 (2%) 4 (9%)
Hyperplasia, Plasma Cell 3 (6%) 2 (4%) 1 (2%) 3 (6%)
Inflammation 1 (2%) 1 (2%)
Necrosis 1 (2%)
Lymph Node, Mesenteric (50) (49) (50) (50) (49)
Angiectasis 1 (2%)
Atrophy 2 (4%) 1 (2%) 1 (2%) 2 (4%)
Ectasia 3 (6%) 5 (10%) 7 (14%) 7 (14%) 7 (14%)
Hemorrhage 2 (4%) 1 (2%)
Necrosis 1 (2%)
Spleen (50) (50) (50) (50) (50)
Accessory Spleen 1 (2%) 1 (2%) 1 (2%)
Angiectasis 1 (2%)
Congestion 6 (12%) 35 (70%) 37 (74%) 30 (60%) 31 (62%)
Fibrosis 5 (10%) 3 (6%) 2 (4%) 2 (4%)
Hematopoietic Cell Proliferation 37 (74%) 45 (90%) 44 (88%) 43 (86%) 39 (78%)
Hemorrhage 2 (4%)
Infarct 1 (2%)
Infiltration Cellular 2 (4%) 1 (2%) 1 (2%)
Necrosis 1 (2%)
Pigmentation 12 (24%) 36 (72%) 38 (76%) 33 (66%) 28 (56%)
Capsule, Fibrosis 1 (2%)
Capsule, Hyperplasia 1 (2%)
Lymphoid Follicle, Atrophy 1 (2%) 2 (4%) 2 (4%) 6 (12%)
Red Pulp, Depletion Cellular 3 (6%) 3 (6%) 2 (4%) 4 (8%) 2 (4%)
Thymus (46) (44) (45) (46) (41)
Atrophy 40 (87%) 43 (98%) 44 (98%) 46 (100%) 41 (100%)
Necrosis 1 (2%)
Epithelial Cell, Hyperplasia 1 (2%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 02/25/99
Route: DOSED FEED Time: 11:42:20
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS MALE 0 PPM 469 PPM 938 PPM 1875 PPM 3750 PPM
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Mammary Gland (45) (47) (46) (50) (47)
Cyst 1 (2%)
Hyperplasia 10 (22%) 21 (45%) 12 (26%) 12 (24%) 10 (21%)
Mineralization 1 (2%)
Duct, Hyperplasia 1 (2%)
Skin (50) (50) (50) (50) (50)
Cyst Epithelial Inclusion 1 (2%) 1 (2%) 1 (2%)
Hemorrhage 1 (2%)
Hyperplasia 1 (2%)
Inflammation 2 (4%)
Dermis, Fibrosis 1 (2%) 1 (2%)
Hair Follicle, Atrophy 1 (2%)
Subcutaneous Tissue, Inflammation,
Granulomatous 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (50) (50) (50) (50) (50)
Fibrous Osteodystrophy 2 (4%) 4 (8%) 8 (16%) 11 (22%) 9 (18%)
Fracture 1 (2%)
Necrosis 1 (2%)
Osteomalacia 13 (26%) 12 (24%) 8 (16%) 3 (6%)
Osteopetrosis 1 (2%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (50) (50) (50) (50) (50)
Hemorrhage 2 (4%) 1 (2%)
Inflammation, Suppurative 1 (2%)
Cerebrum, Degeneration 1 (2%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (50) (50) (50) (50) (50)
Cyst, Squamous 1 (2%)
Foreign Body 1 (2%) 2 (4%) 1 (2%)
Hemorrhage 1 (2%) 2 (4%) 2 (4%)
Inflammation 11 (22%) 13 (26%) 13 (26%) 8 (16%) 14 (28%)
Mineralization 1 (2%) 2 (4%) 3 (6%) 7 (14%) 6 (12%)
Necrosis 1 (2%)
Pigmentation 3 (6%) 6 (12%) 4 (8%) 5 (10%) 5 (10%)
Thrombosis 1 (2%) 2 (4%)
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 02/25/99
Route: DOSED FEED Time: 11:42:20
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS MALE 0 PPM 469 PPM 938 PPM 1875 PPM 3750 PPM
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM - CONT
Alveolar Epithelium, Hyperplasia 3 (6%) 9 (18%) 9 (18%) 7 (14%) 3 (6%)
Mediastinum, Inflammation 1 (2%)
Mediastinum, Thrombosis 1 (2%)
Nose (50) (50) (50) (50) (50)
Foreign Body 4 (8%) 3 (6%) 7 (14%) 5 (10%) 6 (12%)
Hemorrhage 1 (2%)
Inflammation 4 (8%) 3 (6%) 8 (16%) 6 (12%) 6 (12%)
Respiratory Epithelium, Inflammation 1 (2%) 3 (6%)
Trachea (50) (50) (50) (50) (49)
Inflammation 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Eye (2) (4) (5) (2)
Cataract 2 (100%) 2 (50%) 4 (80%)
Degeneration 1 (20%)
Hemorrhage 2 (40%)
Inflammation 1 (25%) 1 (50%)
Mineralization 4 (80%) 1 (50%)
Cornea, Inflammation 1 (25%) 1 (20%)
Retina, Degeneration 2 (100%) 3 (75%) 4 (80%) 1 (50%)
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (50) (50) (50) (50) (50)
Accumulation, Hyaline Droplet 3 (6%) 14 (28%) 10 (20%) 16 (32%) 16 (32%)
Cyst 3 (6%) 4 (8%) 9 (18%) 7 (14%) 13 (26%)
Hydronephrosis 2 (4%) 2 (4%) 2 (4%)
Infarct 1 (2%) 1 (2%)
Inflammation 7 (14%) 5 (10%) 2 (4%) 5 (10%) 4 (8%)
Mineralization, Diffuse 3 (6%) 2 (4%) 9 (18%) 8 (16%) 6 (12%)
Nephropathy 50 (100%) 50 (100%) 50 (100%) 50 (100%) 50 (100%)
Pigmentation 25 (50%) 31 (62%) 36 (72%) 38 (76%) 33 (66%)
Thrombosis 1 (2%)
Medulla, Mineralization 30 (60%) 42 (84%) 46 (92%) 47 (94%) 49 (98%)
Renal Tubule, Hyperplasia 3 (6%) 7 (14%) 3 (6%) 9 (18%) 9 (18%)
Renal Tubule, Hyperplasia, Oncocytic 1 (2%) 1 (2%)
Transitional Epithelium, Hyperplasia 28 (56%) 45 (90%) 44 (88%) 48 (96%) 48 (96%)
Urinary Bladder (50) (50) (50) (50) (49)
Hemorrhage 2 (4%) 1 (2%)
Inflammation 8 (16%) 8 (16%) 11 (22%) 8 (16%) 9 (18%)
Serosa, Inflammation 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 02/25/99
Route: DOSED FEED Time: 11:42:20
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS MALE 0 PPM 469 PPM 938 PPM 1875 PPM 3750 PPM
____________________________________________________________________________________________________________________________________
URINARY SYSTEM - CONT
Transitional Epithelium, Hyperplasia 1 (2%) 1 (2%) 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
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