TDMS Study 88036-04 Pathology Tables
NTP Experiment-Test: 88036-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 05/27/99
Route: DOSED FEED Time: 11:24:12
FINAL #2 CORE STUDY MICE
Facility: Battelle Columbus Laboratory
Chemical CAS #: 84-65-1
Lock Date: 07/01/97
Cage Range: All
Reasons For Removal: All
Removal Date Range: All
Treatment Groups: Include All
a Number of animals examined microscopically at site and number of animals with lesion
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NTP Experiment-Test: 88036-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 05/27/99
Route: DOSED FEED Time: 11:24:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 PPM 833 PPM 2500 PPM 7500 PPM
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 50 50 50 50
Early Deaths
Natural Death 9 5 8 5
Moribund Sacrifice 6 3 4 2
Accidently Killed 3
Survivors
Terminal Sacrifice 35 42 35 42
Missing 1
Animals Examined Microscopically 50 50 50 49
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Esophagus (50) (49) (50) (49)
Inflammation, Chronic 1 (2%)
Intestine Small, Duodenum (50) (50) (50) (48)
Ulcer 1 (2%) 1 (2%)
Liver (49) (50) (50) (49)
Basophilic Focus 1 (2%) 1 (2%) 3 (6%)
Clear Cell Focus 4 (8%) 1 (2%) 1 (2%) 3 (6%)
Degeneration, Diffuse, Fatty 1 (2%) 1 (2%)
Degeneration, Fatty, Focal 2 (4%) 3 (6%) 1 (2%) 9 (18%)
Eosinophilic Focus 6 (12%) 15 (30%) 11 (22%) 22 (45%)
Fatty Change, Focal 5 (10%) 2 (4%) 1 (2%) 3 (6%)
Hematopoietic Cell Proliferation 1 (2%) 1 (2%) 3 (6%) 1 (2%)
Infiltration Cellular, Lymphocyte 3 (6%)
Mineralization, Focal 1 (2%)
Mixed Cell Focus 4 (8%) 2 (4%) 2 (4%) 2 (4%)
Necrosis, Focal 5 (10%) 3 (6%) 1 (2%) 1 (2%)
Tension Lipidosis 1 (2%)
Bile Duct, Cyst 3 (6%) 2 (4%) 2 (4%)
Bile Duct, Hyperplasia 1 (2%)
Centrilobular, Atrophy 1 (2%)
Centrilobular, Degeneration, Fatty 1 (2%) 1 (2%)
Centrilobular, Hypertrophy 1 (2%) 27 (54%) 22 (44%) 39 (80%)
Hepatocyte, Erythrophagocytosis 1 (2%)
Serosa, Inflammation, Chronic 1 (2%)
Mesentery (6) (4) (6) (7)
Inflammation, Chronic 1 (17%)
Inflammation, Chronic Active 1 (17%)
Artery, Thrombosis 1 (17%)
Fat, Necrosis 4 (67%) 3 (75%) 2 (33%) 7 (100%)
Lymphatic, Angiectasis 1 (17%)
Pancreas (50) (50) (50) (49)
Hyperplasia, Focal 2 (4%)
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 05/27/99
Route: DOSED FEED Time: 11:24:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 PPM 833 PPM 2500 PPM 7500 PPM
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Inflammation, Chronic 1 (2%)
Acinus, Atrophy 2 (4%) 1 (2%) 2 (4%) 1 (2%)
Duct, Cyst 1 (2%) 1 (2%)
Stomach, Forestomach (50) (50) (50) (49)
Inflammation, Suppurative 1 (2%)
Ulcer 4 (8%) 1 (2%) 1 (2%) 2 (4%)
Epithelium, Hyperplasia, Focal 1 (2%) 1 (2%) 1 (2%)
Serosa, Inflammation, Chronic 1 (2%)
Stomach, Glandular (50) (50) (50) (49)
Ulcer 1 (2%) 1 (2%)
Epithelium, Hyperplasia 2 (4%)
Epithelium, Hyperplasia, Focal 1 (2%)
Serosa, Inflammation, Chronic 1 (2%)
Tooth (1)
Peridontal Tissue, Inflammation,
Granulomatous 1 (100%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Blood Vessel (50) (50) (49) (49)
Aorta, Mineralization 2 (4%) 1 (2%)
Heart (50) (50) (50) (49)
Thrombosis 1 (2%)
Myocardium, Degeneration 2 (4%) 1 (2%) 1 (2%)
Myocardium, Mineralization 2 (4%)
Valve, Inflammation 1 (2%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (50) (50) (50) (49)
Hyperplasia, Focal 1 (2%) 1 (2%) 2 (4%)
Necrosis 1 (2%)
Adrenal Medulla (50) (50) (49) (49)
Hyperplasia, Focal 1 (2%)
Islets, Pancreatic (50) (50) (50) (49)
Hyperplasia 6 (12%) 13 (26%) 10 (20%) 14 (29%)
Pituitary Gland (47) (50) (48) (46)
Angiectasis 2 (4%) 1 (2%)
Atrophy 1 (2%)
Cyst 1 (2%)
Pars Distalis, Hyperplasia, Focal 8 (17%) 4 (8%) 12 (25%) 6 (13%)
Thyroid Gland (45) (48) (48) (48)
Inflammation, Acute, Focal 1 (2%)
Follicle, Cyst 2 (4%) 1 (2%)
Follicular Cell, Hyperplasia 10 (22%) 14 (29%) 16 (33%) 15 (31%)
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 05/27/99
Route: DOSED FEED Time: 11:24:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 PPM 833 PPM 2500 PPM 7500 PPM
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
Peritoneum (1)
Necrosis 1 (100%)
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Ovary (45) (50) (49) (49)
Angiectasis 2 (4%)
Cyst 3 (7%) 4 (8%) 7 (14%) 5 (10%)
Granulosa Cell, Hyperplasia 1 (2%)
Uterus (50) (50) (50) (49)
Angiectasis 2 (4%) 1 (2%) 1 (2%)
Inflammation, Chronic 1 (2%)
Inflammation, Suppurative 1 (2%) 1 (2%)
Thrombosis 1 (2%)
Endometrium, Hyperplasia, Cystic 35 (70%) 38 (76%) 23 (46%) 39 (80%)
Vagina (1)
Hemorrhage 1 (100%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (50) (50) (50) (49)
Myeloid Cell, Hyperplasia 1 (2%)
Lymph Node (5) (2) (4) (4)
Lumbar, Ectasia 1 (50%)
Lumbar, Hemorrhage 1 (25%)
Mediastinal, Inflammation, Chronic 1 (20%)
Lymph Node, Mandibular (48) (46) (48) (49)
Hyperplasia, Lymphoid 1 (2%)
Inflammation, Suppurative 1 (2%)
Lymph Node, Mesenteric (48) (50) (49) (48)
Ectasia 1 (2%)
Spleen (45) (49) (48) (48)
Hematopoietic Cell Proliferation 9 (20%) 17 (35%) 17 (35%) 26 (54%)
Pigmentation 1 (2%)
Capsule, Inflammation, Chronic 1 (2%)
Lymphoid Follicle, Hyperplasia 8 (18%) 4 (8%) 5 (10%) 6 (13%)
Thymus (44) (47) (46) (44)
Hyperplasia, Lymphoid 1 (2%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 05/27/99
Route: DOSED FEED Time: 11:24:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 PPM 833 PPM 2500 PPM 7500 PPM
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Mammary Gland (48) (48) (48) (49)
Inflammation, Suppurative 1 (2%)
Skin (50) (50) (50) (49)
Hair Follicle, Atrophy, Focal 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (50) (50) (50) (49)
Fibrosis 2 (4%) 2 (4%) 1 (2%) 3 (6%)
Skeletal Muscle (3) (2) (2) (1)
Hemorrhage, Acute 1 (33%)
Inflammation, Chronic 1 (33%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (50) (50) (50) (49)
Hemorrhage 1 (2%)
Hydrocephalus 1 (2%)
Hypothalamus, Compression 1 (2%) 1 (2%) 1 (2%)
Hypothalamus, Degeneration 1 (2%)
Hypothalamus, Necrosis 1 (2%)
Peripheral Nerve (2) (1)
Axon, Degeneration 2 (100%)
Spinal Cord (2) (1)
Axon, Nerve, Degeneration 1 (50%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (50) (50) (50) (49)
Congestion 2 (4%)
Hematopoietic Cell Proliferation 1 (2%)
Hemorrhage 2 (4%) 1 (2%)
Hemorrhage, Chronic, Focal 1 (2%)
Inflammation, Chronic 1 (2%)
Alveolar Epithelium, Hyperplasia, Focal 2 (4%)
Bronchus, Foreign Body 1 (2%)
Interstitium, Inflammation, Chronic, Focal 1 (2%)
Interstitium, Mineralization 1 (2%)
Perivascular, Edema 1 (2%)
Nose (50) (50) (49) (49)
Inflammation, Suppurative 2 (4%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 05/27/99
Route: DOSED FEED Time: 11:24:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 PPM 833 PPM 2500 PPM 7500 PPM
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Eye (2) (3) (1) (1)
Degeneration 1 (50%) 1 (33%) 1 (100%) 1 (100%)
Cornea, Inflammation, Chronic Active 1 (33%)
Lens, Cataract 1 (50%)
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (49) (50) (49) (49)
Glomerulosclerosis 1 (2%)
Infarct 2 (4%)
Inflammation, Suppurative 1 (2%) 1 (2%)
Nephropathy 4 (8%) 3 (6%) 3 (6%) 7 (14%)
Pigmentation 1 (2%)
Pelvis, Inflammation, Chronic Active 1 (2%)
Renal Tubule, Cyst 1 (2%)
Renal Tubule, Hyperplasia, Focal 1 (2%) 1 (2%)
Urinary Bladder (44) (48) (46) (48)
Edema 1 (2%)
Transitional Epithelium, Inclusion Body
Intracytoplasmic 40 (83%) 43 (93%) 46 (96%)
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 05/27/99
Route: DOSED FEED Time: 11:24:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 PPM 833 PPM 2500 PPM 7500 PPM
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 50 50 50 50
Early Deaths
Natural Death 2 6 4 19
Moribund Sacrifice 3 3 3 8
Survivors
Terminal Sacrifice 45 41 43 23
Animals Examined Microscopically 50 50 50 50
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Gallbladder (50) (48) (47) (48)
Cyst 1 (2%)
Liver (50) (50) (50) (49)
Basophilic Focus 3 (6%) 1 (2%)
Clear Cell Focus 14 (28%) 12 (24%) 9 (18%) 2 (4%)
Degeneration, Fatty, Focal 7 (14%) 6 (12%)
Eosinophilic Focus 14 (28%) 17 (34%) 24 (48%) 20 (41%)
Fatty Change, Focal 1 (2%) 1 (2%)
Hematopoietic Cell Proliferation 2 (4%) 4 (8%)
Infarct 1 (2%)
Inflammation, Granulomatous 1 (2%)
Mineralization 2 (4%)
Mixed Cell Focus 3 (6%) 2 (4%) 4 (8%) 1 (2%)
Necrosis, Focal 2 (4%) 3 (6%) 3 (6%) 8 (16%)
Bile Duct, Cyst 1 (2%) 2 (4%) 1 (2%)
Centrilobular, Degeneration, Fatty 1 (2%)
Centrilobular, Hypertrophy 24 (48%) 34 (68%) 41 (82%) 33 (67%)
Hepatocyte, Erythrophagocytosis 1 (2%) 9 (18%) 13 (26%) 6 (12%)
Mesentery (2) (2) (1) (5)
Fat, Necrosis 2 (100%) 2 (100%) 5 (100%)
Pancreas (50) (50) (50) (50)
Inflammation, Acute 1 (2%)
Acinus, Atrophy 2 (4%) 1 (2%) 1 (2%)
Duct, Necrosis 1 (2%)
Stomach, Forestomach (50) (49) (50) (50)
Cyst 1 (2%)
Ulcer 1 (2%) 2 (4%)
Epithelium, Hyperplasia, Focal 2 (4%)
Stomach, Glandular (50) (50) (50) (50)
Foreign Body 1 (2%)
Inflammation, Focal, Suppurative 1 (2%)
Metaplasia, Squamous 1 (2%)
Mineralization 1 (2%)
Ulcer 2 (4%) 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 05/27/99
Route: DOSED FEED Time: 11:24:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 PPM 833 PPM 2500 PPM 7500 PPM
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Epithelium, Hyperplasia, Focal 1 (2%)
Tooth (14) (19) (14) (5)
Inflammation, Suppurative 1 (5%) 1 (7%)
Malformation 14 (100%) 19 (100%) 14 (100%) 5 (100%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (50) (50) (50) (50)
Atrium, Thrombosis 2 (4%)
Myocardium, Degeneration 4 (8%) 3 (6%) 5 (10%)
Myocardium, Mineralization 1 (2%) 1 (2%)
Valve, Fibrosis 1 (2%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (50) (50) (50) (50)
Hyperplasia, Focal 8 (16%) 8 (16%) 8 (16%) 5 (10%)
Subcapsular, Hyperplasia, Focal 1 (2%) 1 (2%) 1 (2%)
Adrenal Medulla (49) (50) (50) (49)
Hyperplasia, Focal 2 (4%)
Islets, Pancreatic (50) (50) (50) (42)
Hyperplasia 40 (80%) 40 (80%) 29 (58%) 17 (40%)
Pituitary Gland (48) (48) (47) (46)
Pars Distalis, Hyperplasia, Focal 1 (2%) 2 (4%)
Thyroid Gland (50) (50) (49) (46)
Follicular Cell, Hyperplasia 7 (14%) 10 (20%) 15 (31%) 21 (46%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Epididymis (50) (50) (50) (50)
Granuloma Sperm 3 (6%)
Hemorrhage 1 (2%)
Preputial Gland (50) (50) (49) (49)
Cyst 22 (44%) 25 (50%) 32 (65%) 22 (45%)
Inflammation, Granulomatous 4 (8%) 1 (2%)
Inflammation, Suppurative 7 (14%) 4 (8%) 4 (8%) 4 (8%)
Prostate (50) (50) (50) (50)
Hyperplasia 1 (2%)
Seminal Vesicle (50) (50) (50) (50)
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 05/27/99
Route: DOSED FEED Time: 11:24:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 PPM 833 PPM 2500 PPM 7500 PPM
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM - CONT
Dilatation 1 (2%)
Inflammation, Chronic 7 (14%) 3 (6%) 4 (8%)
Testes (50) (49) (50) (50)
Germinal Epithelium, Degeneration 2 (4%) 3 (6%)
Germinal Epithelium, Mineralization 2 (4%) 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Lymph Node (1) (1) (3)
Hyperplasia, Lymphoid 1 (100%) 1 (33%)
Lymph Node, Mesenteric (49) (49) (47) (40)
Angiectasis 1 (3%)
Erythrophagocytosis 1 (2%)
Hyperplasia, Lymphoid 1 (2%)
Spleen (50) (50) (49) (42)
Atrophy 1 (2%) 1 (2%)
Hematopoietic Cell Proliferation 12 (24%) 14 (28%) 12 (24%) 30 (71%)
Infiltration Cellular, Mast Cell 1 (2%)
Pigmentation 1 (2%) 1 (2%)
Lymphoid Follicle, Atrophy 1 (2%)
Lymphoid Follicle, Hyperplasia 4 (8%) 1 (2%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Skin (50) (50) (50) (49)
Ulcer 1 (2%) 1 (2%)
Conjunctiva, Inflammation, Chronic 1 (2%)
Prepuce, Hemorrhage 1 (2%)
Subcutaneous Tissue, Edema 1 (2%) 1 (2%)
Subcutaneous Tissue, Inflammation, Chronic
Active 1 (2%)
Subcutaneous Tissue, Inflammation,
Suppurative 1 (2%)
Subcutaneous Tissue, Mineralization 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
None
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 05/27/99
Route: DOSED FEED Time: 11:24:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 PPM 833 PPM 2500 PPM 7500 PPM
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (50) (50) (50) (50)
Meninges, Infiltration Cellular, Lymphocyte 1 (2%)
Peripheral Nerve (1)
Axon, Degeneration 1 (100%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (50) (50) (50) (50)
Inflammation, Chronic 1 (2%)
Inflammation, Granulomatous 1 (2%)
Alveolar Epithelium, Hyperplasia, Focal 1 (2%) 1 (2%) 1 (2%)
Vein, Thrombosis 1 (2%)
Nose (50) (50) (50) (50)
Inflammation, Suppurative 2 (4%) 3 (6%) 1 (2%) 2 (4%)
Trachea (50) (50) (50) (50)
Glands, Hyperplasia 1 (2%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Eye (1) (2)
Degeneration 1 (50%)
Cornea, Inflammation, Chronic 1 (100%) 1 (50%)
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (50) (49) (49) (47)
Degeneration 1 (2%)
Infarct 1 (2%)
Metaplasia, Osseous 1 (2%)
Necrosis 1 (2%)
Nephropathy 29 (58%) 25 (51%) 27 (55%) 18 (38%)
Pigmentation 2 (4%) 2 (4%) 18 (38%)
Glomerulus, Inflammation, Chronic 1 (2%)
Pelvis, Inflammation, Suppurative 1 (2%)
Renal Tubule, Cyst 2 (4%)
Renal Tubule, Hyperplasia, Focal 1 (2%) 1 (2%)
Renal Tubule, Pigmentation, Lipofuscin 1 (2%)
Renal Tubule, Vacuolization Cytoplasmic 1 (2%)
Urinary Bladder (50) (49) (49) (45)
Calculus Gross Observation 1 (2%)
Transitional Epithelium, Inclusion Body
a Number of animals examined microscopically at site and number of animals with lesion
NTP Experiment-Test: 88036-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ANTHRAQUINONE Date: 05/27/99
Route: DOSED FEED Time: 11:24:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 PPM 833 PPM 2500 PPM 7500 PPM
____________________________________________________________________________________________________________________________________
URINARY SYSTEM - CONT
Intracytoplasmic 46 (94%) 46 (94%) 42 (93%)
a Number of animals examined microscopically at site and number of animals with lesion
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