TDMS Study 88070-04 Pathology Tables
NTP Experiment-Test: 88070-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/20/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 12:10:34 CORE STUDY Facility: Battelle Northwest Chemical CAS #: 115-11-0007 Lock Date: 10/05/95 Cage Range: All Reasons For Removal: All Removal Date Range: All Treatment Groups: Include All a Number of animals examined microscopically at site and number of animals with lesion Page 1 NTP Experiment-Test: 88070-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/20/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 12:10:34 ____________________________________________________________________________________________________________________________________ B6C3F1 MICE FEMALE CONTROL 500 PPM 2000 PPM 8000 PPM ____________________________________________________________________________________________________________________________________ DISPOSITION SUMMARY Animals Initially In Study 50 50 50 50 Early Deaths Moribund Sacrifice 13 16 6 13 Natural Death 5 3 5 4 Survivors Terminal Sacrifice 32 31 39 33 Animals Examined Microscopically 50 50 50 50 ____________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM Intestine Large, Rectum (45) (45) (47) (48) Artery, Inflammation, Chronic Active 1 (2%) Intestine Small, Ileum (46) (48) (46) (47) Inflammation, Chronic Active 1 (2%) Liver (47) (50) (50) (50) Angiectasis 1 (2%) 1 (2%) 1 (2%) Basophilic Focus 4 (9%) 4 (8%) 2 (4%) 5 (10%) Clear Cell Focus 4 (8%) 3 (6%) 4 (8%) Cyst 1 (2%) 1 (2%) Degeneration, Fatty 2 (4%) 1 (2%) 5 (10%) 1 (2%) Eosinophilic Focus 17 (36%) 11 (22%) 12 (24%) 7 (14%) Hematopoietic Cell Proliferation 1 (2%) 1 (2%) Mixed Cell Focus 1 (2%) Necrosis 2 (4%) 3 (6%) 1 (2%) Tension Lipidosis 1 (2%) 3 (6%) 1 (2%) Bile Duct, Hyperplasia 1 (2%) Centrilobular, Necrosis 1 (2%) 3 (6%) Mesentery (7) (8) (8) (10) Artery, Inflammation 1 (13%) 1 (10%) Fat, Inflammation, Chronic Active 1 (10%) Fat, Necrosis 7 (100%) 6 (75%) 6 (75%) 10 (100%) Pancreas (47) (50) (48) (49) Atrophy 1 (2%) Basophilic Focus 1 (2%) 2 (4%) Lipomatosis 1 (2%) 1 (2%) Duct, Cyst 1 (2%) 1 (2%) Stomach, Forestomach (48) (49) (49) (49) Hyperplasia 2 (4%) 4 (8%) 1 (2%) 1 (2%) Inflammation, Acute 1 (2%) 2 (4%) Stomach, Glandular (46) (48) (48) (49) Necrosis 1 (2%) Artery, Inflammation, Chronic Active 1 (2%) Tooth (1) (1) Malformation 1 (100%) 1 (100%) a Number of animals examined microscopically at site and number of animals with lesion Page 2 NTP Experiment-Test: 88070-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/20/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 12:10:34 ____________________________________________________________________________________________________________________________________ B6C3F1 MICE FEMALE CONTROL 500 PPM 2000 PPM 8000 PPM ____________________________________________________________________________________________________________________________________ CARDIOVASCULAR SYSTEM Heart (50) (50) (50) (50) Cardiomyopathy 10 (20%) 10 (20%) 12 (24%) 8 (16%) Mineralization 1 (2%) ____________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM Adrenal Cortex (49) (50) (49) (50) Hematopoietic Cell Proliferation 1 (2%) 1 (2%) 1 (2%) Hyperplasia 3 (6%) 4 (8%) 3 (6%) 5 (10%) Hypertrophy 3 (6%) 1 (2%) 4 (8%) 2 (4%) Vacuolization Cytoplasmic 1 (2%) 2 (4%) 1 (2%) 2 (4%) Adrenal Medulla (48) (49) (49) (49) Hyperplasia 3 (6%) Islets, Pancreatic (47) (49) (48) (49) Hyperplasia 1 (2%) 1 (2%) 3 (6%) Pituitary Gland (49) (49) (49) (49) Pars Distalis, Hyperplasia 13 (27%) 15 (31%) 19 (39%) 14 (29%) Thyroid Gland (48) (50) (49) (50) Follicular Cell, Hyperplasia 25 (52%) 16 (32%) 29 (59%) 30 (60%) ____________________________________________________________________________________________________________________________________ GENERAL BODY SYSTEM None ____________________________________________________________________________________________________________________________________ GENITAL SYSTEM Ovary (48) (50) (48) (50) Angiectasis 1 (2%) 3 (6%) Cyst 13 (27%) 14 (28%) 11 (23%) 8 (16%) Inflammation, Suppurative 1 (2%) Uterus (49) (50) (50) (50) Angiectasis 1 (2%) Hydrometra 6 (12%) 7 (14%) 3 (6%) 4 (8%) Inflammation, Suppurative 1 (2%) 1 (2%) Necrosis 1 (2%) Thrombosis 1 (2%) Endometrium, Hyperplasia, Cystic 1 (2%) ____________________________________________________________________________________________________________________________________ a Number of animals examined microscopically at site and number of animals with lesion Page 3 NTP Experiment-Test: 88070-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/20/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 12:10:34 ____________________________________________________________________________________________________________________________________ B6C3F1 MICE FEMALE CONTROL 500 PPM 2000 PPM 8000 PPM ____________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM Bone Marrow (48) (49) (49) (50) Necrosis 1 (2%) Lymph Node (7) (2) (5) (4) Lumbar, Hemorrhage 1 (14%) Lymph Node, Bronchial (38) (33) (44) (44) Hyperplasia, Plasma Cell 1 (3%) Lymph Node, Mandibular (41) (42) (44) (38) Hyperplasia, Lymphoid 1 (2%) Infiltration Cellular, Mixed Cell 1 (2%) Lymph Node, Mesenteric (46) (48) (47) (49) Angiectasis 1 (2%) 2 (4%) Infiltration Cellular, Plasma Cell 1 (2%) Infiltration Cellular, Mixed Cell 1 (2%) Lymph Node, Mediastinal (38) (42) (36) (42) Infiltration Cellular, Mixed Cell 1 (3%) Spleen (49) (50) (50) (50) Hematopoietic Cell Proliferation 4 (8%) 12 (24%) 6 (12%) 5 (10%) Infarct 1 (2%) Infiltration Cellular, Histiocyte 1 (2%) ____________________________________________________________________________________________________________________________________ INTEGUMENTARY SYSTEM Skin (49) (50) (49) (50) Inflammation, Acute 1 (2%) Inflammation, Chronic Active 1 (2%) 1 (2%) 1 (2%) ____________________________________________________________________________________________________________________________________ MUSCULOSKELETAL SYSTEM None ____________________________________________________________________________________________________________________________________ NERVOUS SYSTEM Brain (49) (50) (50) (50) Inflammation, Chronic Active 1 (2%) ____________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM Lung (49) (50) (50) (50) Hemorrhage 1 (2%) Infiltration Cellular, Histiocyte 1 (2%) 1 (2%) Inflammation, Granulomatous 1 (2%) Metaplasia, Osseous 1 (2%) a Number of animals examined microscopically at site and number of animals with lesion Page 4 NTP Experiment-Test: 88070-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/20/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 12:10:34 ____________________________________________________________________________________________________________________________________ B6C3F1 MICE FEMALE CONTROL 500 PPM 2000 PPM 8000 PPM ____________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - CONT Alveolar Epithelium, Hyperplasia 4 (8%) 3 (6%) 3 (6%) 1 (2%) Nose (47) (50) (49) (50) Inflammation, Chronic 1 (2%) Inflammation, Suppurative 1 (2%) 1 (2%) Olfactory Epithelium, Atrophy 1 (2%) Olfactory Epithelium, Degeneration, Hyaline 17 (36%) 19 (38%) 24 (49%) 27 (54%) Respiratory Epithelium, Degeneration, Hyaline 21 (45%) 39 (78%) 41 (84%) 48 (96%) ____________________________________________________________________________________________________________________________________ SPECIAL SENSES SYSTEM Eye (1) (1) (1) Degeneration 1 (100%) Cornea, Metaplasia, Squamous 1 (100%) ____________________________________________________________________________________________________________________________________ URINARY SYSTEM Kidney (49) (50) (49) (50) Amyloid Deposition 1 (2%) Cyst 1 (2%) Infarct 1 (2%) 2 (4%) 1 (2%) Metaplasia, Osseous 1 (2%) 1 (2%) 2 (4%) Mineralization 1 (2%) Nephropathy 13 (27%) 14 (28%) 18 (37%) 12 (24%) Renal Tubule, Necrosis 1 (2%) Urinary Bladder (45) (49) (49) (50) Artery, Inflammation, Chronic Active 1 (2%) a Number of animals examined microscopically at site and number of animals with lesion Page 5 NTP Experiment-Test: 88070-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/20/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 12:10:34 ____________________________________________________________________________________________________________________________________ B6C3F1 MICE MALE CONTROL 500 PPM 2000 PPM 8000 PPM ____________________________________________________________________________________________________________________________________ DISPOSITION SUMMARY Animals Initially In Study 50 50 50 50 Early Deaths Natural Death 6 9 3 9 Moribund Sacrifice 16 9 20 13 Survivors Terminal Sacrifice 28 32 27 28 Animals Examined Microscopically 50 50 50 50 ____________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM Liver (50) (50) (50) (49) Basophilic Focus 7 (14%) 8 (16%) 6 (12%) 5 (10%) Clear Cell Focus 9 (18%) 4 (8%) 6 (12%) 10 (20%) Cyst 1 (2%) 1 (2%) Degeneration, Fatty 2 (4%) 1 (2%) 2 (4%) 5 (10%) Eosinophilic Focus 5 (10%) 4 (8%) 11 (22%) 7 (14%) Mixed Cell Focus 2 (4%) 1 (2%) Necrosis 1 (2%) 5 (10%) 2 (4%) 1 (2%) Vacuolization Cytoplasmic, Focal 1 (2%) 1 (2%) 1 (2%) Centrilobular, Necrosis 2 (4%) Mesentery (4) (2) (4) (2) Fat, Inflammation, Chronic Active 1 (25%) Fat, Necrosis 3 (75%) 2 (100%) 4 (100%) 2 (100%) Pancreas (50) (48) (50) (47) Atrophy 1 (2%) Basophilic Focus 1 (2%) Stomach, Forestomach (50) (49) (50) (48) Hyperplasia 2 (4%) 1 (2%) 1 (2%) Inflammation, Acute 1 (2%) 1 (2%) Stomach, Glandular (49) (47) (49) (46) Inflammation, Acute 1 (2%) Necrosis 2 (4%) 1 (2%) Tooth (7) (14) (9) (16) Malformation 6 (86%) 12 (86%) 7 (78%) 14 (88%) ____________________________________________________________________________________________________________________________________ CARDIOVASCULAR SYSTEM Heart (50) (50) (50) (50) Cardiomyopathy 21 (42%) 18 (36%) 16 (32%) 19 (38%) Inflammation, Chronic 1 (2%) Thrombosis 1 (2%) 1 (2%) ____________________________________________________________________________________________________________________________________ a Number of animals examined microscopically at site and number of animals with lesion Page 6 NTP Experiment-Test: 88070-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/20/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 12:10:34 ____________________________________________________________________________________________________________________________________ B6C3F1 MICE MALE CONTROL 500 PPM 2000 PPM 8000 PPM ____________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM Adrenal Cortex (49) (49) (50) (49) Hyperplasia 14 (29%) 10 (20%) 15 (30%) 16 (33%) Hypertrophy 32 (65%) 24 (49%) 24 (48%) 29 (59%) Adrenal Medulla (49) (49) (50) (49) Hyperplasia 1 (2%) 1 (2%) Islets, Pancreatic (50) (48) (50) (48) Hyperplasia 1 (2%) 1 (2%) 1 (2%) Pituitary Gland (50) (48) (49) (48) Pars Distalis, Hyperplasia 1 (2%) 4 (8%) 3 (6%) 4 (8%) Thyroid Gland (50) (50) (50) (49) Follicular Cell, Hyperplasia 5 (10%) 6 (12%) 3 (6%) 3 (6%) ____________________________________________________________________________________________________________________________________ GENERAL BODY SYSTEM None ____________________________________________________________________________________________________________________________________ GENITAL SYSTEM Epididymis (50) (50) (50) (50) Granuloma Sperm 1 (2%) 1 (2%) 5 (10%) Penis (2) Inflammation, Acute 1 (50%) Preputial Gland (50) (48) (50) (50) Cyst 2 (4%) 1 (2%) Inflammation, Chronic Active 2 (4%) 3 (6%) Prostate (48) (46) (47) (48) Inflammation, Chronic Active 2 (4%) 4 (9%) 4 (8%) Seminal Vesicle (49) (47) (50) (47) Necrosis 1 (2%) Testes (50) (50) (50) (50) Atrophy 1 (2%) 1 (2%) Mineralization 1 (2%) ____________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM Bone Marrow (50) (49) (50) (49) Congestion 1 (2%) Hemorrhage 1 (2%) Lymph Node (4) (3) (7) (1) Iliac, Infiltration Cellular, Plasma Cell 1 (33%) 2 (29%) 1 (100%) Iliac, Infiltration Cellular, Polymorphonuclear 1 (14%) Lumbar, Infiltration Cellular, Plasma Cell 1 (33%) a Number of animals examined microscopically at site and number of animals with lesion Page 7 NTP Experiment-Test: 88070-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/20/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 12:10:34 ____________________________________________________________________________________________________________________________________ B6C3F1 MICE MALE CONTROL 500 PPM 2000 PPM 8000 PPM ____________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - CONT Pancreatic, Infiltration Cellular, Plasma Cell 1 (25%) Lymph Node, Mesenteric (48) (49) (48) (49) Angiectasis 1 (2%) 1 (2%) Infiltration Cellular, Plasma Cell 1 (2%) Spleen (50) (49) (50) (48) Hematopoietic Cell Proliferation 4 (8%) 3 (6%) 5 (10%) 4 (8%) Infiltration Cellular, Histiocyte 1 (2%) 1 (2%) ____________________________________________________________________________________________________________________________________ INTEGUMENTARY SYSTEM Skin (49) (50) (50) (50) Cyst 1 (2%) Prepuce, Inflammation, Chronic Active 7 (14%) 6 (12%) 13 (26%) 9 (18%) Subcutaneous Tissue, Edema 1 (2%) ____________________________________________________________________________________________________________________________________ MUSCULOSKELETAL SYSTEM None ____________________________________________________________________________________________________________________________________ NERVOUS SYSTEM Peripheral Nerve (1) Sciatic, Degeneration 1 (100%) ____________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM Lung (50) (50) (50) (50) Hemorrhage 3 (6%) Thrombosis 1 (2%) 1 (2%) Alveolar Epithelium, Hyperplasia 5 (10%) 6 (12%) 4 (8%) 3 (6%) Nose (50) (49) (50) (48) Inflammation, Suppurative 1 (2%) 2 (4%) 2 (4%) Polyp, Inflammatory 1 (2%) 1 (2%) Nasolacrimal Duct, Polyp, Inflammatory 1 (2%) Olfactory Epithelium, Atrophy 1 (2%) 1 (2%) 1 (2%) 2 (4%) Olfactory Epithelium, Degeneration, Hyaline 6 (12%) 7 (14%) 16 (32%) 17 (35%) Respiratory Epithelium, Degeneration, Hyaline 6 (12%) 19 (39%) 29 (58%) 39 (81%) ____________________________________________________________________________________________________________________________________ a Number of animals examined microscopically at site and number of animals with lesion Page 8 NTP Experiment-Test: 88070-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/20/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 12:10:34 ____________________________________________________________________________________________________________________________________ B6C3F1 MICE MALE CONTROL 500 PPM 2000 PPM 8000 PPM ____________________________________________________________________________________________________________________________________ SPECIAL SENSES SYSTEM Eye (2) (2) Cataract 1 (50%) Cornea, Inflammation, Chronic Active 1 (50%) Harderian Gland (4) (4) (4) (4) Hyperplasia 1 (25%) ____________________________________________________________________________________________________________________________________ URINARY SYSTEM Kidney (50) (49) (50) (50) Cyst 1 (2%) 1 (2%) Infarct 1 (2%) 2 (4%) 4 (8%) Inflammation, Chronic Active 1 (2%) Metaplasia, Osseous 1 (2%) 2 (4%) Mineralization 1 (2%) 1 (2%) Nephropathy 36 (72%) 39 (80%) 38 (76%) 40 (80%) Capsule, Inflammation, Chronic 1 (2%) Papilla, Inflammation, Suppurative 3 (6%) 3 (6%) 6 (12%) 5 (10%) Pelvis, Dilatation 3 (6%) 2 (4%) 4 (8%) 4 (8%) Renal Tubule, Hyperplasia 1 (2%) 1 (2%) Urinary Bladder (49) (47) (49) (46) Calculus Gross Observation 1 (2%) Inflammation, Chronic Active 4 (8%) 3 (6%) 7 (14%) 6 (13%) a Number of animals examined microscopically at site and number of animals with lesion Page 9 ------------------------------------------------------------ ---------- END OF REPORT ---------- ------------------------------------------------------------