TDMS Study 93005-03 Pathology Tables
NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 Facility: National Center for Toxicological Research Chemical CAS #: 569-64-2 Lock Date: None Cage Range: All Reasons For Removal: All Removal Date Range: All Treatment Groups: Include All Note: Animals arranged according to days on test Page 1 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 _____________________________________________________________________________________________________________________ | 4| 4| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 0| 4| 2| 2| 3| 3| 5| 8| 0| 2| 2| 3| 4| 4| 4| 6| 0| 1| 2| 2| 2| 2| 2| 2| 2| | 1| 3| 6| 9| 3| 5| 2| 9| 6| 6| 6| 1| 1| 1| 7| 7| 4| 1| 2| 8| 8| 8| 8| 8| 8| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 0| 0| 0| 0| 0| 0| 0 PPM | 1| 1| 2| 2| 2| 2| 2| 4| 4| 5| 5| 6| 6| 7| 7| 7| 8| 3| 3| 9| 9| 9| 9| 9| 9| | 2| 4| 7| 8| 5| 6| 9| 0| 6| 1| 4| 6| 4| 0| 2| 5| 2| 3| 2| 1| 2| 3| 4| 5| 6| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Colon | + + + + + + A + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Cecum | + + + + + + A + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Duodenum | + + + + + + A + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Jejunum | + + + + + + A + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Ileum | + + + + + + A + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Adenoma, Multiple | | |__________________________________________________________________________| Mesentery | + + + + + | Sarcoma | X | |__________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Tongue | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 2 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 _____________________________________________________________________________________________________________________ | 4| 4| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 0| 4| 2| 2| 3| 3| 5| 8| 0| 2| 2| 3| 4| 4| 4| 6| 0| 1| 2| 2| 2| 2| 2| 2| 2| | 1| 3| 6| 9| 3| 5| 2| 9| 6| 6| 6| 1| 1| 1| 7| 7| 4| 1| 2| 8| 8| 8| 8| 8| 8| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 0| 0| 0| 0| 0| 0| 0 PPM | 1| 1| 2| 2| 2| 2| 2| 4| 4| 5| 5| 6| 6| 7| 7| 7| 8| 3| 3| 9| 9| 9| 9| 9| 9| | 2| 4| 7| 8| 5| 6| 9| 0| 6| 1| 4| 6| 4| 0| 2| 5| 2| 3| 2| 1| 2| 3| 4| 5| 6| __________________________________________|__________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Pheochromocytoma Malignant, | | Metastatic, Adrenal Medulla | X | |__________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Pheochromocytoma Malignant | X | |__________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Parathyroid Gland | M + + + + + + + + + + + + + M M M + + + + + + + M| |__________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Pars Distalis, Adenoma | X X X X X X X X X X X X X X | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + M M + + + + + + + +| C-Cell, Adenoma | X | C-Cell, Carcinoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X X | Carcinoma | X X X X X| |__________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Polyp Stromal | X X X X X X X| Sarcoma Stromal | X | |__________________________________________________________________________| Vagina | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 3 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 _____________________________________________________________________________________________________________________ | 4| 4| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 0| 4| 2| 2| 3| 3| 5| 8| 0| 2| 2| 3| 4| 4| 4| 6| 0| 1| 2| 2| 2| 2| 2| 2| 2| | 1| 3| 6| 9| 3| 5| 2| 9| 6| 6| 6| 1| 1| 1| 7| 7| 4| 1| 2| 8| 8| 8| 8| 8| 8| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 0| 0| 0| 0| 0| 0| 0 PPM | 1| 1| 2| 2| 2| 2| 2| 4| 4| 5| 5| 6| 6| 7| 7| 7| 8| 3| 3| 9| 9| 9| 9| 9| 9| | 2| 4| 7| 8| 5| 6| 9| 0| 6| 1| 4| 6| 4| 0| 2| 5| 2| 3| 2| 1| 2| 3| 4| 5| 6| __________________________________________|__________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Lymph Node | + + + + + + + + | |__________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + M + + + + + + +| |__________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Thymus | M M M + M + + + + + M + + + + + + + + + + + M + +| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma | X | Fibroadenoma | X X X X X X X X| |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Basal Cell Adenoma | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skeletal Muscle | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Peripheral Nerve | + + M + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Spinal Cord | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | __________________________________________|__________________________________________________________________________| Page 4 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 _____________________________________________________________________________________________________________________ | 4| 4| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 0| 4| 2| 2| 3| 3| 5| 8| 0| 2| 2| 3| 4| 4| 4| 6| 0| 1| 2| 2| 2| 2| 2| 2| 2| | 1| 3| 6| 9| 3| 5| 2| 9| 6| 6| 6| 1| 1| 1| 7| 7| 4| 1| 2| 8| 8| 8| 8| 8| 8| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 0| 0| 0| 0| 0| 0| 0 PPM | 1| 1| 2| 2| 2| 2| 2| 4| 4| 5| 5| 6| 6| 7| 7| 7| 8| 3| 3| 9| 9| 9| 9| 9| 9| | 2| 4| 7| 8| 5| 6| 9| 0| 6| 1| 4| 6| 4| 0| 2| 5| 2| 3| 2| 1| 2| 3| 4| 5| 6| __________________________________________|__________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Sarcoma, Metastatic, Mesentery | X | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | + + + + + + M + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Harderian Gland | + + + + + + M + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Lacrimal Gland | + + + + + + + + +| |__________________________________________________________________________| Zymbal's Gland | + + + M + + + + + + + + + + + + + + + + + + + + +| Carcinoma | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + M + + + + + + +| __________________________________________|__________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X X X X| __________________________________________|__________________________________________________________________________| Page 5 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 0 PPM | 9| 1| 1| 1| 1| 1| 1| 1| 3| 3| 3| 3| 3| 6| 6| 6| 6| 6| 8| 8| 9| 9| 9| | L | | 7| 1| 2| 3| 4| 5| 6| 7| 4| 5| 6| 7| 8| 2| 3| 4| 5| 6| 8| 9| 0| 1| 2| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Hepatocellular Adenoma, Multiple | X | 1 | |__________________________________________________________________________|____________| Mesentery | + + + + | 9 | Sarcoma | | 1 | |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Tongue | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 6 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 0 PPM | 9| 1| 1| 1| 1| 1| 1| 1| 3| 3| 3| 3| 3| 6| 6| 6| 6| 6| 8| 8| 9| 9| 9| | L | | 7| 1| 2| 3| 4| 5| 6| 7| 4| 5| 6| 7| 8| 2| 3| 4| 5| 6| 8| 9| 0| 1| 2| | | _____________________________________________________________________________________________________________________|____________| CARDIOVASCULAR SYSTEM - cont | | | | | | Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Adenoma | X | 2 | Pheochromocytoma Malignant, | | | Metastatic, Adrenal Medulla | | 1 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + M + + + + + + + + + + | 47 | Pheochromocytoma Malignant | | 1 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + | 43 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Pars Distalis, Adenoma | X X X X X X X X X X X X | 26 | |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + | 46 | C-Cell, Adenoma | X X X | 4 | C-Cell, Carcinoma | X X | 3 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Adenoma | X X X X | 7 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 7 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 0 PPM | 9| 1| 1| 1| 1| 1| 1| 1| 3| 3| 3| 3| 3| 6| 6| 6| 6| 6| 8| 8| 9| 9| 9| | L | | 7| 1| 2| 3| 4| 5| 6| 7| 4| 5| 6| 7| 8| 2| 3| 4| 5| 6| 8| 9| 0| 1| 2| | | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | | | | Carcinoma | | 5 | |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Polyp Stromal | X X X X X X X | 14 | Sarcoma Stromal | X | 2 | |__________________________________________________________________________|____________| Vagina | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Lymph Node | + + | 10 | |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + I + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Thymus | M + + + + + + + + + + + + + + + + + + + + + M | 40 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + M + + + + + + + + + + + + + M + + + | 46 | Carcinoma | X | 2 | Fibroadenoma | X X X X X X X | 15 | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Basal Cell Adenoma | | 1 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 8 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 0 PPM | 9| 1| 1| 1| 1| 1| 1| 1| 3| 3| 3| 3| 3| 6| 6| 6| 6| 6| 8| 8| 9| 9| 9| | L | | 7| 1| 2| 3| 4| 5| 6| 7| 4| 5| 6| 7| 8| 2| 3| 4| 5| 6| 8| 9| 0| 1| 2| | | _____________________________________________________________________________________________________________________|____________| MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Skeletal Muscle | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Peripheral Nerve | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Spinal Cord | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Sarcoma, Metastatic, Mesentery | | 1 | |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Lacrimal Gland | + + + + + + + + + | 18 | |__________________________________________________________________________|____________| Zymbal's Gland | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Carcinoma | | 1 | _____________________________________________________________________________________________________________________| | * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 9 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 0 PPM | 9| 1| 1| 1| 1| 1| 1| 1| 3| 3| 3| 3| 3| 6| 6| 6| 6| 6| 8| 8| 9| 9| 9| | L | | 7| 1| 2| 3| 4| 5| 6| 7| 4| 5| 6| 7| 8| 2| 3| 4| 5| 6| 8| 9| 0| 1| 2| | | _____________________________________________________________________________________________________________________|____________| URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + | 47 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Leukemia Mononuclear | X X X X X | 19 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 10 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 _____________________________________________________________________________________________________________________ | 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| DAY ON TEST | 4| 8| 9| 1| 3| 4| 5| 7| 7| 9| 9| 9| 0| 1| 1| 2| 4| 4| 8| 8| 0| 0| 1| 1| 2| | 3| 5| 4| 2| 9| 0| 6| 4| 7| 1| 6| 8| 0| 0| 3| 4| 1| 8| 6| 7| 7| 8| 8| 8| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 0| 1| 1| 100 PPM | 1| 1| 2| 2| 2| 2| 3| 4| 3| 4| 4| 4| 4| 4| 4| 6| 6| 6| 8| 8| 8| 3| 8| 6| 5| | 3| 8| 1| 2| 3| 4| 5| 1| 8| 8| 2| 5| 9| 3| 7| 7| 2| 5| 6| 1| 5| 1| 9| 1| 9| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Colon | A + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | A + + + + + + + + + + + + + + + + + + + A + + + +| |__________________________________________________________________________| Intestine Large, Cecum | A + + + + + + + + + + + + + + + + + + + A + + + +| |__________________________________________________________________________| Intestine Small, Duodenum | A + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Jejunum | A + + + + + + + + + + + + + + + + + + + A + + + +| |__________________________________________________________________________| Intestine Small, Ileum | A + + + + + + + + + + + + + + + + + + + A + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Uterus | X | Hepatocellular Adenoma | | |__________________________________________________________________________| Mesentery | + + + + | Carcinoma, Metastatic, Uterus | X | |__________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Uterus | X | |__________________________________________________________________________| Salivary Glands | + + + + + M + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Tongue | + + + + + + + + + + + + + + + + + + + + + + + + +| Squamous Cell Papilloma | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Blood Vessel | + + + + + + + + + + + + + M + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 11 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 _____________________________________________________________________________________________________________________ | 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| DAY ON TEST | 4| 8| 9| 1| 3| 4| 5| 7| 7| 9| 9| 9| 0| 1| 1| 2| 4| 4| 8| 8| 0| 0| 1| 1| 2| | 3| 5| 4| 2| 9| 0| 6| 4| 7| 1| 6| 8| 0| 0| 3| 4| 1| 8| 6| 7| 7| 8| 8| 8| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 0| 1| 1| 100 PPM | 1| 1| 2| 2| 2| 2| 3| 4| 3| 4| 4| 4| 4| 4| 4| 6| 6| 6| 8| 8| 8| 3| 8| 6| 5| | 3| 8| 1| 2| 3| 4| 5| 1| 8| 8| 2| 5| 9| 3| 7| 7| 2| 5| 6| 1| 5| 1| 9| 1| 9| __________________________________________|__________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | | | |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Adrenal Medulla | + + + + + + + + M + + + + + + + + + + + + + + + +| Pheochromocytoma Benign | | |__________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | |__________________________________________________________________________| Parathyroid Gland | + + + + + M + + + + + + M M + + + + M + + M + + +| |__________________________________________________________________________| Pituitary Gland | I + + + + + + + + + + + + + + + + + + + + + + + +| Pars Distalis, Adenoma | X X X X X X X X X X X X X X X| |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| C-Cell, Adenoma | X | C-Cell, Carcinoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | |__________________________________________________________________________| Tissue NOS | + | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X| Carcinoma | | Bilateral, Carcinoma | | |__________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Granulosa Cell Tumor Malignant | | |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 12 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 _____________________________________________________________________________________________________________________ | 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| DAY ON TEST | 4| 8| 9| 1| 3| 4| 5| 7| 7| 9| 9| 9| 0| 1| 1| 2| 4| 4| 8| 8| 0| 0| 1| 1| 2| | 3| 5| 4| 2| 9| 0| 6| 4| 7| 1| 6| 8| 0| 0| 3| 4| 1| 8| 6| 7| 7| 8| 8| 8| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 0| 1| 1| 100 PPM | 1| 1| 2| 2| 2| 2| 3| 4| 3| 4| 4| 4| 4| 4| 4| 6| 6| 6| 8| 8| 8| 3| 8| 6| 5| | 3| 8| 1| 2| 3| 4| 5| 1| 8| 8| 2| 5| 9| 3| 7| 7| 2| 5| 6| 1| 5| 1| 9| 1| 9| __________________________________________|__________________________________________________________________________| GENITAL SYSTEM - cont | | | | Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Polyp Stromal | X X X X | Sarcoma Stromal | | Endometrium, Carcinoma | X | |__________________________________________________________________________| Vagina | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Lymph Node | + + + + + | |__________________________________________________________________________| Lymph Node, Mandibular | + + + + + M + + + + + M + + + + + + + + + + + + +| |__________________________________________________________________________| Lymph Node, Mesenteric | A + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Uterus | X | |__________________________________________________________________________| Thymus | + + + + M + M + + M + + + + + + + + M + + + + + M| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma | X | Fibroadenoma | X X X X X X| |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Fibroma | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skeletal Muscle | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 13 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 _____________________________________________________________________________________________________________________ | 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| DAY ON TEST | 4| 8| 9| 1| 3| 4| 5| 7| 7| 9| 9| 9| 0| 1| 1| 2| 4| 4| 8| 8| 0| 0| 1| 1| 2| | 3| 5| 4| 2| 9| 0| 6| 4| 7| 1| 6| 8| 0| 0| 3| 4| 1| 8| 6| 7| 7| 8| 8| 8| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 0| 1| 1| 100 PPM | 1| 1| 2| 2| 2| 2| 3| 4| 3| 4| 4| 4| 4| 4| 4| 6| 6| 6| 8| 8| 8| 3| 8| 6| 5| | 3| 8| 1| 2| 3| 4| 5| 1| 8| 8| 2| 5| 9| 3| 7| 7| 2| 5| 6| 1| 5| 1| 9| 1| 9| __________________________________________|__________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Peripheral Nerve | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Spinal Cord | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | | Carcinoma, Metastatic, Thyroid Gland | X | Sarcoma Stromal, Metastatic, Uterus | | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Lacrimal Gland | + + + + + + | |__________________________________________________________________________| Zymbal's Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Transitional Epithelium, Papilloma | X | __________________________________________|__________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X X X | __________________________________________|__________________________________________________________________________| Page 14 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 100 PPM | 9| 9| 0| 0| 0| 1| 1| 1| 3| 4| 4| 4| 4| 6| 6| 6| 7| 7| 8| 9| 9| 9| 9| | L | | 8| 9| 0| 1| 9| 0| 8| 9| 9| 0| 1| 2| 3| 7| 8| 9| 0| 1| 4| 3| 4| 5| 6| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + | 46 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + | 46 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + | 46 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + | 46 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Carcinoma, Metastatic, Uterus | | 1 | Hepatocellular Adenoma | X | 1 | |__________________________________________________________________________|____________| Mesentery | + | 5 | Carcinoma, Metastatic, Uterus | | 1 | |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Carcinoma, Metastatic, Uterus | | 1 | |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Tongue | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Squamous Cell Papilloma | X | 1 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 15 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 100 PPM | 9| 9| 0| 0| 0| 1| 1| 1| 3| 4| 4| 4| 4| 6| 6| 6| 7| 7| 8| 9| 9| 9| 9| | L | | 8| 9| 0| 1| 9| 0| 8| 9| 9| 0| 1| 2| 3| 7| 8| 9| 0| 1| 4| 3| 4| 5| 6| | | _____________________________________________________________________________________________________________________|____________| CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Pheochromocytoma Benign | X | 1 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Adenoma | X X | 2 | |__________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + | 43 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Pars Distalis, Adenoma | X X X X X X X X X X X X X X X X X X X X X | 36 | |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + | 48 | C-Cell, Adenoma | X X X X | 5 | C-Cell, Carcinoma | | 1 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | |__________________________________________________________________________|____________| Tissue NOS | | 1 | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Adenoma | X X X X | 5 | Carcinoma | X | 1 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 16 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 100 PPM | 9| 9| 0| 0| 0| 1| 1| 1| 3| 4| 4| 4| 4| 6| 6| 6| 7| 7| 8| 9| 9| 9| 9| | L | | 8| 9| 0| 1| 9| 0| 8| 9| 9| 0| 1| 2| 3| 7| 8| 9| 0| 1| 4| 3| 4| 5| 6| | | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | | | | Bilateral, Carcinoma | X | 1 | |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Granulosa Cell Tumor Malignant | X | 1 | |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Polyp Stromal | X X X | 7 | Sarcoma Stromal | X | 1 | Endometrium, Carcinoma | | 1 | |__________________________________________________________________________|____________| Vagina | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Lymph Node | + + | 7 | |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + | 46 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Carcinoma, Metastatic, Uterus | | 1 | |__________________________________________________________________________|____________| Thymus | + + + + + + + + M + + + + + + + + + + + + + + | 42 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Carcinoma | X | 2 | Fibroadenoma | X X X X X X X | 13 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 17 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 100 PPM | 9| 9| 0| 0| 0| 1| 1| 1| 3| 4| 4| 4| 4| 6| 6| 6| 7| 7| 8| 9| 9| 9| 9| | L | | 8| 9| 0| 1| 9| 0| 8| 9| 9| 0| 1| 2| 3| 7| 8| 9| 0| 1| 4| 3| 4| 5| 6| | | _____________________________________________________________________________________________________________________|____________| INTEGUMENTARY SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Subcutaneous Tissue, Fibroma | X | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Skeletal Muscle | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Peripheral Nerve | + + + + M + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Spinal Cord | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Alveolar/Bronchiolar Adenoma | X | 1 | Carcinoma, Metastatic, Thyroid Gland | | 1 | Sarcoma Stromal, Metastatic, Uterus | X | 1 | |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 18 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 100 PPM | 9| 9| 0| 0| 0| 1| 1| 1| 3| 4| 4| 4| 4| 6| 6| 6| 7| 7| 8| 9| 9| 9| 9| | L | | 8| 9| 0| 1| 9| 0| 8| 9| 9| 0| 1| 2| 3| 7| 8| 9| 0| 1| 4| 3| 4| 5| 6| | | _____________________________________________________________________________________________________________________|____________| SPECIAL SENSES SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Lacrimal Gland | + + + + + + + + | 14 | |__________________________________________________________________________|____________| Zymbal's Gland | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Transitional Epithelium, Papilloma | | 1 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Leukemia Mononuclear | X X X X | 17 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 19 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 _____________________________________________________________________________________________________________________ | 4| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 8| 0| 0| 1| 2| 2| 5| 5| 5| 6| 7| 9| 9| 9| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| | 4| 8| 3| 9| 6| 7| 2| 9| 9| 6| 3| 2| 7| 7| 5| 9| 8| 8| 8| 8| 8| 8| 8| 8| 8| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 300 PPM | 1| 1| 5| 6| 5| 5| 6| 6| 7| 7| 8| 8| 7| 9| 6| 8| 0| 0| 2| 2| 2| 2| 2| 2| 2| | 7| 9| 0| 0| 3| 6| 8| 9| 3| 1| 0| 8| 8| 0| 0| 7| 2| 3| 0| 1| 2| 3| 4| 5| 6| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| Polyp Adenomatous | | |__________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Carcinoma | | Hepatocellular Adenoma | X | |__________________________________________________________________________| Mesentery | + + | |__________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Tongue | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 20 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 _____________________________________________________________________________________________________________________ | 4| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 8| 0| 0| 1| 2| 2| 5| 5| 5| 6| 7| 9| 9| 9| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| | 4| 8| 3| 9| 6| 7| 2| 9| 9| 6| 3| 2| 7| 7| 5| 9| 8| 8| 8| 8| 8| 8| 8| 8| 8| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 300 PPM | 1| 1| 5| 6| 5| 5| 6| 6| 7| 7| 8| 8| 7| 9| 6| 8| 0| 0| 2| 2| 2| 2| 2| 2| 2| | 7| 9| 0| 0| 3| 6| 8| 9| 3| 1| 0| 8| 8| 0| 0| 7| 2| 3| 0| 1| 2| 3| 4| 5| 6| __________________________________________|__________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | Carcinoma | | |__________________________________________________________________________| Parathyroid Gland | M + + + + + + + + + M + + + + + + + + + + + + + +| |__________________________________________________________________________| Pituitary Gland | M + + + + + + + + + + + I + + + + + + + + + + + +| Pars Distalis, Adenoma | X X X X X X X X X X X X X X X | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| C-Cell, Adenoma | X X| C-Cell, Carcinoma | X X | Follicular Cell, Adenoma | | Follicular Cell, Carcinoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + + M| Adenoma | X X X | Carcinoma | X X X | Bilateral, Carcinoma | | |__________________________________________________________________________| Ovary | + + + + + + + + M + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Clitoral Gland| X | |__________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 21 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 _____________________________________________________________________________________________________________________ | 4| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 8| 0| 0| 1| 2| 2| 5| 5| 5| 6| 7| 9| 9| 9| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| | 4| 8| 3| 9| 6| 7| 2| 9| 9| 6| 3| 2| 7| 7| 5| 9| 8| 8| 8| 8| 8| 8| 8| 8| 8| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 300 PPM | 1| 1| 5| 6| 5| 5| 6| 6| 7| 7| 8| 8| 7| 9| 6| 8| 0| 0| 2| 2| 2| 2| 2| 2| 2| | 7| 9| 0| 0| 3| 6| 8| 9| 3| 1| 0| 8| 8| 0| 0| 7| 2| 3| 0| 1| 2| 3| 4| 5| 6| __________________________________________|__________________________________________________________________________| GENITAL SYSTEM - cont | | | | Polyp Stromal | X X | Sarcoma Stromal | X | |__________________________________________________________________________| Vagina | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Lymph Node | + + + + + | |__________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + M + + + + +| |__________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Thymus | + + + + + + + + + + + + M + M + + + M + + + + M +| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma | | Fibroadenoma | X X X X X | |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Keratoacanthoma | | Squamous Cell Papilloma | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skeletal Muscle | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | __________________________________________|__________________________________________________________________________| Page 22 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 _____________________________________________________________________________________________________________________ | 4| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 8| 0| 0| 1| 2| 2| 5| 5| 5| 6| 7| 9| 9| 9| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| | 4| 8| 3| 9| 6| 7| 2| 9| 9| 6| 3| 2| 7| 7| 5| 9| 8| 8| 8| 8| 8| 8| 8| 8| 8| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 300 PPM | 1| 1| 5| 6| 5| 5| 6| 6| 7| 7| 8| 8| 7| 9| 6| 8| 0| 0| 2| 2| 2| 2| 2| 2| 2| | 7| 9| 0| 0| 3| 6| 8| 9| 3| 1| 0| 8| 8| 0| 0| 7| 2| 3| 0| 1| 2| 3| 4| 5| 6| __________________________________________|__________________________________________________________________________| NERVOUS SYSTEM - cont | | | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Peripheral Nerve | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Spinal Cord | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Lacrimal Gland | + + + + + + + + + + + + + +| |__________________________________________________________________________| Zymbal's Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + M + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X | __________________________________________|__________________________________________________________________________| Page 23 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| | A | 300 PPM | 2| 4| 4| 4| 4| 4| 4| 5| 7| 7| 7| 7| 7| 7| 7| 7| 9| 9| 9| 0| 0| 0| 0| | L | | 7| 4| 5| 6| 7| 8| 9| 0| 2| 3| 4| 5| 6| 7| 8| 9| 7| 8| 9| 0| 1| 2| 3| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Polyp Adenomatous | X | 1 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Hepatocellular Carcinoma | X | 1 | Hepatocellular Adenoma | X X | 3 | |__________________________________________________________________________|____________| Mesentery | | 2 | |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Tongue | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 24 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| | A | 300 PPM | 2| 4| 4| 4| 4| 4| 4| 5| 7| 7| 7| 7| 7| 7| 7| 7| 9| 9| 9| 0| 0| 0| 0| | L | | 7| 4| 5| 6| 7| 8| 9| 0| 2| 3| 4| 5| 6| 7| 8| 9| 7| 8| 9| 0| 1| 2| 3| | | _____________________________________________________________________________________________________________________|____________| CARDIOVASCULAR SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Adenoma | X | 1 | Carcinoma | X | 1 | |__________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + M + + | 45 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + | 46 | Pars Distalis, Adenoma | X X X X X X X X X X X X X X X X X | 32 | |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + M + + | 47 | C-Cell, Adenoma | X | 3 | C-Cell, Carcinoma | | 2 | Follicular Cell, Adenoma | X | 1 | Follicular Cell, Carcinoma | X | 2 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + | 47 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 25 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| | A | 300 PPM | 2| 4| 4| 4| 4| 4| 4| 5| 7| 7| 7| 7| 7| 7| 7| 7| 9| 9| 9| 0| 0| 0| 0| | L | | 7| 4| 5| 6| 7| 8| 9| 0| 2| 3| 4| 5| 6| 7| 8| 9| 7| 8| 9| 0| 1| 2| 3| | | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | | | | Adenoma | X X X X X | 8 | Carcinoma | X X | 5 | Bilateral, Carcinoma | X | 1 | |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Carcinoma, Metastatic, Clitoral Gland| | 1 | |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Polyp Stromal | X X X | 5 | Sarcoma Stromal | | 1 | |__________________________________________________________________________|____________| Vagina | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Lymph Node | + + | 7 | |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Thymus | + + M + + + + + + + + + + + + + + + + + + + + | 43 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Carcinoma | X | 1 | Fibroadenoma | X X X X X X X | 12 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 26 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| | A | 300 PPM | 2| 4| 4| 4| 4| 4| 4| 5| 7| 7| 7| 7| 7| 7| 7| 7| 9| 9| 9| 0| 0| 0| 0| | L | | 7| 4| 5| 6| 7| 8| 9| 0| 2| 3| 4| 5| 6| 7| 8| 9| 7| 8| 9| 0| 1| 2| 3| | | _____________________________________________________________________________________________________________________|____________| INTEGUMENTARY SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Keratoacanthoma | X | 1 | Squamous Cell Papilloma | X | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Skeletal Muscle | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Peripheral Nerve | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Spinal Cord | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Alveolar/Bronchiolar Adenoma | X | 1 | |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 27 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| | A | 300 PPM | 2| 4| 4| 4| 4| 4| 4| 5| 7| 7| 7| 7| 7| 7| 7| 7| 9| 9| 9| 0| 0| 0| 0| | L | | 7| 4| 5| 6| 7| 8| 9| 0| 2| 3| 4| 5| 6| 7| 8| 9| 7| 8| 9| 0| 1| 2| 3| | | _____________________________________________________________________________________________________________________|____________| SPECIAL SENSES SYSTEM - cont | | | | | | Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Lacrimal Gland | + | 15 | |__________________________________________________________________________|____________| Zymbal's Gland | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Carcinoma | | 1 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + | 47 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Leukemia Mononuclear | X X X | 10 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 28 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 _____________________________________________________________________________________________________________________ | 1| 3| 3| 4| 4| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| DAY ON TEST | 1| 2| 7| 4| 5| 0| 4| 6| 7| 8| 8| 0| 1| 2| 3| 3| 3| 5| 7| 8| 9| 9| 9| 2| 2| | 1| 3| 8| 3| 4| 8| 6| 2| 0| 1| 9| 5| 9| 0| 0| 3| 4| 4| 0| 5| 4| 7| 9| 8| 8| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 600 PPM | 0| 0| 1| 1| 1| 2| 3| 3| 3| 3| 4| 5| 5| 5| 6| 5| 6| 7| 7| 8| 7| 7| 8| 0| 0| | 1| 6| 1| 6| 5| 0| 6| 7| 4| 9| 4| 2| 9| 5| 1| 8| 3| 4| 6| 3| 7| 9| 4| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Colon | + + A + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| Polyp Adenomatous | | |__________________________________________________________________________| Intestine Large, Cecum | + + A + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Jejunum | + + A + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Ileum | + + A + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Adenoma | | Hepatocellular Adenoma, Multiple | X| Sarcoma, Metastatic, Ovary | X | |__________________________________________________________________________| Mesentery | + + | Sarcoma, Metastatic, Ovary | X | |__________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Tongue | + + + + + + + + + + + + + + + + + + + + + + + + +| Sarcoma, Metastatic, Ovary | X | Squamous Cell Carcinoma | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | __________________________________________|__________________________________________________________________________| Page 29 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 _____________________________________________________________________________________________________________________ | 1| 3| 3| 4| 4| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| DAY ON TEST | 1| 2| 7| 4| 5| 0| 4| 6| 7| 8| 8| 0| 1| 2| 3| 3| 3| 5| 7| 8| 9| 9| 9| 2| 2| | 1| 3| 8| 3| 4| 8| 6| 2| 0| 1| 9| 5| 9| 0| 0| 3| 4| 4| 0| 5| 4| 7| 9| 8| 8| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 600 PPM | 0| 0| 1| 1| 1| 2| 3| 3| 3| 3| 4| 5| 5| 5| 6| 5| 6| 7| 7| 8| 7| 7| 8| 0| 0| | 1| 6| 1| 6| 5| 0| 6| 7| 4| 9| 4| 2| 9| 5| 1| 8| 3| 4| 6| 3| 7| 9| 4| 4| 5| __________________________________________|__________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | | | |__________________________________________________________________________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Sarcoma, Metastatic, Ovary | X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Sarcoma, Metastatic, Ovary | X | |__________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Pheochromocytoma Benign | | Sarcoma, Metastatic, Ovary | X | |__________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | |__________________________________________________________________________| Parathyroid Gland | + + + + + + + + M + M + + M + M + M + M + + + + +| |__________________________________________________________________________| Pituitary Gland | + + + + M + M + + + + + + + + + + + I + + + + + +| Carcinoma, Metastatic, Zymbal's Gland| X | Pars Distalis, Adenoma | X X X X X X X X X| Pars Distalis, Carcinoma | X | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + M + + + + +| Sarcoma, Metastatic, Ovary | X | C-Cell, Adenoma | | C-Cell, Carcinoma | | Follicular Cell, Adenoma | | Follicular Cell, Carcinoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | __________________________________________|__________________________________________________________________________| Page 30 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 _____________________________________________________________________________________________________________________ | 1| 3| 3| 4| 4| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| DAY ON TEST | 1| 2| 7| 4| 5| 0| 4| 6| 7| 8| 8| 0| 1| 2| 3| 3| 3| 5| 7| 8| 9| 9| 9| 2| 2| | 1| 3| 8| 3| 4| 8| 6| 2| 0| 1| 9| 5| 9| 0| 0| 3| 4| 4| 0| 5| 4| 7| 9| 8| 8| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 600 PPM | 0| 0| 1| 1| 1| 2| 3| 3| 3| 3| 4| 5| 5| 5| 6| 5| 6| 7| 7| 8| 7| 7| 8| 0| 0| | 1| 6| 1| 6| 5| 0| 6| 7| 4| 9| 4| 2| 9| 5| 1| 8| 3| 4| 6| 3| 7| 9| 4| 4| 5| __________________________________________|__________________________________________________________________________| GENITAL SYSTEM - cont | | | | |__________________________________________________________________________| Clitoral Gland | + + + + + + + + + + + + + M + + + + + + + + + + +| Adenoma | X X X X | Carcinoma | X X X X | Bilateral, Carcinoma | | |__________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Sarcoma | X | |__________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Polyp Stromal | X X X X X | Sarcoma, Metastatic, Ovary | X | Schwannoma Malignant | X | Endometrium, Adenoma | | |__________________________________________________________________________| Vagina | + + + + + + + + + + + + + + + + + + + + + + + + +| Sarcoma | X| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Lymph Node | + + + | |__________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemangiosarcoma | | Sarcoma, Metastatic, Ovary | X | |__________________________________________________________________________| Thymus | + + M + + + M M M + + + + + + + + + + M M + + + +| Sarcoma, Metastatic, Ovary | X | Sarcoma, Metastatic, Uncertain | | Primary Site | | __________________________________________|__________________________________________________________________________| Page 31 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 _____________________________________________________________________________________________________________________ | 1| 3| 3| 4| 4| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| DAY ON TEST | 1| 2| 7| 4| 5| 0| 4| 6| 7| 8| 8| 0| 1| 2| 3| 3| 3| 5| 7| 8| 9| 9| 9| 2| 2| | 1| 3| 8| 3| 4| 8| 6| 2| 0| 1| 9| 5| 9| 0| 0| 3| 4| 4| 0| 5| 4| 7| 9| 8| 8| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 600 PPM | 0| 0| 1| 1| 1| 2| 3| 3| 3| 3| 4| 5| 5| 5| 6| 5| 6| 7| 7| 8| 7| 7| 8| 0| 0| | 1| 6| 1| 6| 5| 0| 6| 7| 4| 9| 4| 2| 9| 5| 1| 8| 3| 4| 6| 3| 7| 9| 4| 4| 5| __________________________________________|__________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma | X X X | Fibroadenoma | X X X X | |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Sarcoma | X X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Sarcoma | | |__________________________________________________________________________| Skeletal Muscle | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Oligodendroglioma Malignant | | Ventricle, Carcinoma, Metastatic, | | Pituitary Gland | X | |__________________________________________________________________________| Peripheral Nerve | + + + + + + + + + + + + + + + M + + + + + + + + +| |__________________________________________________________________________| Spinal Cord | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Zymbal's Gland| X | Sarcoma, Metastatic, Uncertain | | Primary Site | | Mediastinum, Sarcoma, Metastatic, | | Ovary | X | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 32 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 _____________________________________________________________________________________________________________________ | 1| 3| 3| 4| 4| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| DAY ON TEST | 1| 2| 7| 4| 5| 0| 4| 6| 7| 8| 8| 0| 1| 2| 3| 3| 3| 5| 7| 8| 9| 9| 9| 2| 2| | 1| 3| 8| 3| 4| 8| 6| 2| 0| 1| 9| 5| 9| 0| 0| 3| 4| 4| 0| 5| 4| 7| 9| 8| 8| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 600 PPM | 0| 0| 1| 1| 1| 2| 3| 3| 3| 3| 4| 5| 5| 5| 6| 5| 6| 7| 7| 8| 7| 7| 8| 0| 0| | 1| 6| 1| 6| 5| 0| 6| 7| 4| 9| 4| 2| 9| 5| 1| 8| 3| 4| 6| 3| 7| 9| 4| 4| 5| __________________________________________|__________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Ear | | Pinna, Neural Crest Tumor | | |__________________________________________________________________________| Eye | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | |__________________________________________________________________________| Lacrimal Gland | + + +| |__________________________________________________________________________| Zymbal's Gland | M + + + + + + + + + + + + + + + + + + + M + + + +| Carcinoma | X X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Sarcoma, Metastatic, Skin | X | Transitional Epithelium, Carcinoma | | __________________________________________|__________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________|__________________________________________________________________________| Page 33 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | A | 600 PPM | 0| 0| 0| 2| 2| 3| 5| 5| 5| 5| 5| 5| 5| 5| 8| 8| 8| 8| 0| 0| 0| 0| 0| | L | | 6| 7| 8| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Polyp Adenomatous | X | 1 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Hepatocellular Adenoma | X X X | 3 | Hepatocellular Adenoma, Multiple | | 1 | Sarcoma, Metastatic, Ovary | | 1 | |__________________________________________________________________________|____________| Mesentery | + + | 4 | Sarcoma, Metastatic, Ovary | | 1 | |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Tongue | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 34 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | A | 600 PPM | 0| 0| 0| 2| 2| 3| 5| 5| 5| 5| 5| 5| 5| 5| 8| 8| 8| 8| 0| 0| 0| 0| 0| | L | | 6| 7| 8| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | | | | Sarcoma, Metastatic, Ovary | | 1 | Squamous Cell Carcinoma | X | 1 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Sarcoma, Metastatic, Ovary | | 1 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Sarcoma, Metastatic, Ovary | | 1 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Pheochromocytoma Benign | X | 1 | Sarcoma, Metastatic, Ovary | | 1 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Adenoma | X | 1 | |__________________________________________________________________________|____________| Parathyroid Gland | + + + M + + M + + + M + + + + + + + + + + + + | 39 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + | 45 | Carcinoma, Metastatic, Zymbal's Gland| | 1 | Pars Distalis, Adenoma | X X X X X X X X X X X X X X X X X X X X | 29 | Pars Distalis, Carcinoma | | 1 | |__________________________________________________________________________|____________| Thyroid Gland | + + + M + + + + + + + + + + + + + + + + + + + | 46 | Sarcoma, Metastatic, Ovary | | 1 | C-Cell, Adenoma | X X X | 3 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 35 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | A | 600 PPM | 0| 0| 0| 2| 2| 3| 5| 5| 5| 5| 5| 5| 5| 5| 8| 8| 8| 8| 0| 0| 0| 0| 0| | L | | 6| 7| 8| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| ENDOCRINE SYSTEM - cont | | | | | | C-Cell, Carcinoma | X | 1 | Follicular Cell, Adenoma | X | 1 | Follicular Cell, Carcinoma | X | 1 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Adenoma | X X X X | 8 | Carcinoma | | 4 | Bilateral, Carcinoma | X | 1 | |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Sarcoma | | 1 | |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Polyp Stromal | X X X X X X X X X X X | 16 | Sarcoma, Metastatic, Ovary | | 1 | Schwannoma Malignant | | 1 | Endometrium, Adenoma | X | 1 | |__________________________________________________________________________|____________| Vagina | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Sarcoma | | 1 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Lymph Node | + + | 5 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 36 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | A | 600 PPM | 0| 0| 0| 2| 2| 3| 5| 5| 5| 5| 5| 5| 5| 5| 8| 8| 8| 8| 0| 0| 0| 0| 0| | L | | 6| 7| 8| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | | | | Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Hemangiosarcoma | X | 1 | Sarcoma, Metastatic, Ovary | | 1 | |__________________________________________________________________________|____________| Thymus | + + + M + + + + + + + M + + + + + + + + + + + | 40 | Sarcoma, Metastatic, Ovary | | 1 | Sarcoma, Metastatic, Uncertain | | | Primary Site | X | 1 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Carcinoma | X X | 5 | Fibroadenoma | X X X X X | 9 | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Subcutaneous Tissue, Sarcoma | | 2 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Sarcoma | X | 1 | |__________________________________________________________________________|____________| Skeletal Muscle | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 37 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | A | 600 PPM | 0| 0| 0| 2| 2| 3| 5| 5| 5| 5| 5| 5| 5| 5| 8| 8| 8| 8| 0| 0| 0| 0| 0| | L | | 6| 7| 8| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| NERVOUS SYSTEM - cont | | | | | | Oligodendroglioma Malignant | X | 1 | Ventricle, Carcinoma, Metastatic, | | | Pituitary Gland | | 1 | |__________________________________________________________________________|____________| Peripheral Nerve | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Spinal Cord | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Carcinoma, Metastatic, Zymbal's Gland| | 1 | Sarcoma, Metastatic, Uncertain | | | Primary Site | X | 1 | Mediastinum, Sarcoma, Metastatic, | | | Ovary | | 1 | |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Ear | + | 1 | Pinna, Neural Crest Tumor | X | 1 | |__________________________________________________________________________|____________| Eye | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Adenoma | | 1 | |__________________________________________________________________________|____________| Lacrimal Gland | + + + + + + | 9 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 38 NTP Experiment-Test: 93005-03 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: CHRONIC MALACHITE GREEN Date: 06/18/03 Route: DOSED FEED Time: 14:30:09 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | A | 600 PPM | 0| 0| 0| 2| 2| 3| 5| 5| 5| 5| 5| 5| 5| 5| 8| 8| 8| 8| 0| 0| 0| 0| 0| | L | | 6| 7| 8| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| SPECIAL SENSES SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Zymbal's Gland | + + + + + + + + + + + + + + + + + + + + + + + | 46 | Carcinoma | | 2 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + M + + | 47 | Sarcoma, Metastatic, Skin | | 1 | Transitional Epithelium, Carcinoma | X | 1 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Leukemia Mononuclear | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 39 ------------------------------------------------------------ ---------- END OF REPORT ---------- ------------------------------------------------------------