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TDMS Study 93005-04 Pathology Tables

NTP Experiment-Test: 93005-04  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                          MALACHITE GREEN                                      Date: 06/19/03
Route: DOSED FEED                                                                                                 Time: 07:21:07




       Facility:  National Center for Toxicological Research

       Chemical CAS #:  569-64-2

       Lock Date:  None

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All




































a  Number of animals examined microscopically at site and number of animals with lesion

                                                              Page   1


NTP Experiment-Test: 93005-04  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                          MALACHITE GREEN                                      Date: 06/19/03  
Route: DOSED FEED                                                                                                 Time: 07:21:07  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         C57BL/6N XC3H/HEN MTN-NCTR FEMALE         0 PPM        100 PPM      225 PPM      450 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially in Study                          48           48           48           48                                     
  Early Deaths                                                                                                                      
    Natural Death                                      4            1            4            3                                     
    Accidently Killed                                                            1                                                  
    Moribund Sacrifice                                 4            3            3            4                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                40           44           40           41                                     
                                                                                                                                    
  Animals Examined Microscopically                    48           48           48           48                                     
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Gallbladder                                        (43)         (46)         (44)         (43)                                   
   Intestine Large, Colon                             (48)         (46)         (44)         (46)                                   
   Intestine Large, Rectum                            (46)         (46)         (46)         (48)                                   
   Intestine Large, Cecum                             (46)         (47)         (45)         (46)                                   
   Intestine Small, Duodenum                          (46)         (47)         (43)         (47)                                   
      Polyp Adenomatous                                                          1 (2%)                                             
   Intestine Small, Jejunum                           (46)         (47)         (44)         (47)                                   
   Intestine Small, Ileum                             (46)         (47)         (44)         (46)                                   
   Liver                                              (48)         (48)         (46)         (48)                                   
      Hemangiosarcoma                                               1 (2%)                                                          
      Hepatocellular Carcinoma                         1 (2%)       2 (4%)                    1 (2%)                                
      Hepatocellular Adenoma                           3 (6%)       2 (4%)       2 (4%)       2 (4%)                                
      Histiocytic Sarcoma                              3 (6%)                    1 (2%)       1 (2%)                                
      Sarcoma, Metastatic, Skin                                     1 (2%)                                                          
      Sarcoma, Metastatic, Uterus                      1 (2%)                                                                       
   Mesentery                                          (2)          (5)          (1)                                                 
      Sarcoma, Metastatic, Skin                                     1 (20%)                                                         
      Sarcoma, Metastatic, Uterus                      1 (50%)                                                                      
   Pancreas                                           (47)         (47)         (44)         (47)                                   
      Histiocytic Sarcoma                              2 (4%)                                                                       
      Sarcoma, Metastatic, Ovary                       1 (2%)                                                                       
   Salivary Glands                                    (48)         (48)         (48)         (48)                                   
   Stomach, Forestomach                               (47)         (47)         (46)         (48)                                   
      Squamous Cell Papilloma                          2 (4%)       1 (2%)       2 (4%)       1 (2%)                                
   Stomach, Glandular                                 (47)         (47)         (46)         (48)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   2                                                               
                                                                                                                                   
NTP Experiment-Test: 93005-04  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                          MALACHITE GREEN                                      Date: 06/19/03  
Route: DOSED FEED                                                                                                 Time: 07:21:07  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         C57BL/6N XC3H/HEN MTN-NCTR FEMALE         0 PPM        100 PPM      225 PPM      450 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (48)         (48)         (48)         (48)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (47)         (47)         (45)         (47)                                   
      Sarcoma, Metastatic, Skin                                                  1 (2%)                                             
      Subcapsular, Adenoma                                                                    1 (2%)                                
   Adrenal Medulla                                    (47)         (46)         (44)         (46)                                   
      Pheochromocytoma Benign                                       2 (4%)       1 (2%)       1 (2%)                                
   Islets, Pancreatic                                 (46)         (46)         (43)         (47)                                   
      Adenoma                                                                                 1 (2%)                                
      Carcinoma                                        1 (2%)                                                                       
   Pituitary Gland                                    (48)         (47)         (46)         (45)                                   
      Pars Distalis, Adenoma                                        2 (4%)       2 (4%)                                             
   Thyroid Gland                                      (47)         (46)         (46)         (48)                                   
      Follicular Cell, Adenoma                         1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   Tissue NOS                                         (1)                       (1)                                                 
      Hemangiosarcoma                                  1 (100%)                                                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Ovary                                              (48)         (46)         (46)         (45)                                   
      Granulosa Cell Tumor Benign                                   2 (4%)                                                          
      Histiocytic Sarcoma                              2 (4%)                                                                       
      Luteoma                                          1 (2%)                                                                       
      Sarcoma                                          1 (2%)                                                                       
      Sarcoma, Metastatic, Uterus                      1 (2%)                                                                       
      Yolk Sac Carcinoma                                            1 (2%)                                                          
   Uterus                                             (48)         (48)         (47)         (48)                                   
      Adenoma                                          1 (2%)                                                                       
      Histiocytic Sarcoma                              2 (4%)                    1 (2%)                                             
      Polyp Stromal                                                              1 (2%)                                             
      Sarcoma                                          1 (2%)                                                                       
   Vagina                                             (48)         (48)         (46)         (48)                                   
      Leiomyoma                                                                  1 (2%)                                             
      Sarcoma                                          1 (2%)                                                                       
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   3                                                               
                                                                                                                                   
NTP Experiment-Test: 93005-04  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                          MALACHITE GREEN                                      Date: 06/19/03  
Route: DOSED FEED                                                                                                 Time: 07:21:07  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         C57BL/6N XC3H/HEN MTN-NCTR FEMALE         0 PPM        100 PPM      225 PPM      450 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
GENITAL SYSTEM - cont                                                                                                               
      Squamous Cell Carcinoma                          1 (2%)                    1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (47)         (47)         (47)         (48)                                   
      Hemangiosarcoma                                  1 (2%)       1 (2%)       1 (2%)                                             
   Lymph Node                                         (13)         (13)         (6)          (9)                                    
      Lumbar, Histiocytic Sarcoma                      1 (8%)                    1 (17%)                                            
      Pancreatic, Sarcoma, Metastatic, Skin                         1 (8%)                                                          
      Renal, Histiocytic Sarcoma                       1 (8%)                    1 (17%)                                            
      Renal, Sarcoma, Metastatic, Skin                              1 (8%)                                                          
   Lymph Node, Mandibular                             (48)         (48)         (47)         (48)                                   
   Lymph Node, Mesenteric                             (45)         (46)         (44)         (46)                                   
      Histiocytic Sarcoma                              2 (4%)                                 1 (2%)                                
      Sarcoma, Metastatic, Ovary                       1 (2%)                                                                       
      Sarcoma, Metastatic, Uterus                      1 (2%)                                                                       
   Spleen                                             (47)         (47)         (45)         (47)                                   
      Hemangiosarcoma                                  1 (2%)       1 (2%)       1 (2%)       1 (2%)                                
      Histiocytic Sarcoma                              2 (4%)                                 1 (2%)                                
      Sarcoma, Metastatic, Ovary                       1 (2%)                                                                       
   Thymus                                             (43)         (44)         (43)         (47)                                   
      Histiocytic Sarcoma                              2 (5%)                                 1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (46)         (48)         (45)         (46)                                   
      Carcinoma                                        2 (4%)       1 (2%)       1 (2%)       4 (9%)                                
   Skin                                               (48)         (48)         (46)         (48)                                   
      Hemangiosarcoma                                                                         1 (2%)                                
      Sarcoma                                                                    2 (4%)       1 (2%)                                
      Subcutaneous Tissue, Sarcoma                                  1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Skeletal Muscle                                    (48)         (48)         (48)         (48)                                   
      Sarcoma, Metastatic, Skin                                     1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   4                                                               
                                                                                                                                   
NTP Experiment-Test: 93005-04  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                          MALACHITE GREEN                                      Date: 06/19/03  
Route: DOSED FEED                                                                                                 Time: 07:21:07  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         C57BL/6N XC3H/HEN MTN-NCTR FEMALE         0 PPM        100 PPM      225 PPM      450 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (48)         (48)         (48)         (48)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (48)         (48)         (48)         (48)                                   
      Alveolar/Bronchiolar Adenoma                     2 (4%)       4 (8%)       3 (6%)                                             
      Alveolar/Bronchiolar Carcinoma                                1 (2%)                                                          
      Alveolar/Bronchiolar Carcinoma, Multiple                                   1 (2%)                                             
      Carcinoma, Metastatic, Mammary Gland                                                    1 (2%)                                
      Histiocytic Sarcoma                              3 (6%)                                 1 (2%)                                
      Sarcoma, Metastatic, Skin                                                  1 (2%)       1 (2%)                                
   Nose                                               (48)         (48)         (48)         (48)                                   
   Trachea                                            (48)         (47)         (46)         (48)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Harderian Gland                                    (48)         (48)         (48)         (48)                                   
      Adenoma                                          3 (6%)                    2 (4%)       3 (6%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (48)         (47)         (48)         (47)                                   
      Histiocytic Sarcoma                                                                     1 (2%)                                
      Sarcoma, Metastatic, Ovary                       1 (2%)                                                                       
   Urinary Bladder                                    (47)         (46)         (45)         (48)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SYSTEMIC LESIONS                                                                                                                   
                                                                                                                                    
   Multiple Organs                                   *(48)        *(48)        *(48)        *(48)                                   
      Histiocytic Sarcoma                              3 (6%)                    1 (2%)       1 (2%)                                
      Leukemia Granulocytic                            1 (2%)                                                                       
      Lymphoma Malignant                               7 (15%)     10 (21%)      6 (13%)     13 (27%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
     *   Number of animals with any tissue examined microscopically                                                                 
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   5                                                               
                                                                                                                                   
NTP Experiment-Test: 93005-04  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                          MALACHITE GREEN                                      Date: 06/19/03  
Route: DOSED FEED                                                                                                 Time: 07:21:07  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         C57BL/6N XC3H/HEN MTN-NCTR FEMALE         0 PPM        100 PPM      225 PPM      450 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
TUMOR SUMMARY                                                                                                                       
                                                                                                                                    
   Total Animals with Primary Neoplasms (b)            25          23          20          25                                       
     Total Primary Neoplasms                           35          32          29          31                                       
                                                                                                                                    
   Total Animals with Benign Neoplasms                 12          11          11           8                                       
     Total Benign Neoplasms                            13          13          15           9                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms              18          15          12          20                                       
     Total Malignant Neoplasms                         22          19          14          22                                       
                                                                                                                                    
   Total Animals with Metastatic Neoplasms              2           1           1           2                                       
     Total Metastatic Neoplasm                          8           5           2           2                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms                                                                                           
   Uncertain Primary Site                                                                                                           
                                                                                                                                    
   Total Animals with Neoplasms Uncertain-                                                                                          
   Benign or Malignant                                                                                                              
     Total Uncertain Neoplasms                                                                                                      
                                                                                                                                    
                                                                                                                                    
____________________________________________________________________________________________________________________________________
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Primary tumors: all tumors except metastatic tumors                                                                              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   6                                                               
                                                                                                                                   
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                             ----------              END OF REPORT             ----------                                           
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