TDMS Study 93023-07 Pathology Tables
NTP Experiment-Test: 93023-07 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 2,4-HEXADIENAL Date: 02/15/01
Route: GAVAGE Time: 11:07:14
FINAL #1/MICE
Facility: Southern Research Institute
Chemical CAS #: 142-83-6
Lock Date: 02/24/00
Cage Range: All
Reasons For Removal: All
Removal Date Range: All
Treatment Groups: Include All
a Number of animals examined microscopically at site and number of animals with lesion
Page 1
NTP Experiment-Test: 93023-07 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 2,4-HEXADIENAL Date: 02/15/01
Route: GAVAGE Time: 11:07:14
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 30 60 120
MG/KG/DY MG/KG/DY MG/KG/DY MG/KG/DY
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 50 50 50 50
Early Deaths
Natural Death 6 4 5 6
Moribund Sacrifice 2 8 8 4
Dosing Accident 1
Survivors
Terminal Sacrifice 42 37 37 37
Natural Death 2
Missing 1
Animals Examined Microscopically 50 49 50 50
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Esophagus (49) (49) (50) (50)
Perforation 1 (2%)
Epithelium, Hyperplasia, Squamous 1 (2%)
Periesophageal Tissue, Foreign Body 1 (2%)
Gallbladder (44) (45) (47) (41)
Cyst 3 (7%) 1 (2%) 2 (5%)
Hyperplasia, Lymphoid 1 (2%)
Liver (50) (49) (50) (50)
Angiectasis 1 (2%) 2 (4%)
Basophilic Focus 2 (4%)
Congestion, Focal 1 (2%) 1 (2%) 1 (2%)
Fibrosis, Focal 1 (2%)
Hemorrhage, Focal 1 (2%) 1 (2%)
Hyperplasia, Focal, Lymphoid 1 (2%) 1 (2%)
Hyperplasia, Lymphoid 2 (4%) 1 (2%)
Infarct, Chronic 1 (2%)
Infiltration Cellular, Mixed Cell 41 (82%) 35 (71%) 36 (72%) 31 (62%)
Mineralization, Focal 1 (2%)
Mixed Cell Focus 2 (4%)
Mixed Cell Focus, Multiple 1 (2%)
Tension Lipidosis 1 (2%)
Bile Duct, Cyst 1 (2%) 1 (2%)
Bile Duct, Hyperplasia 1 (2%) 1 (2%)
Bile Duct, Hyperplasia, Cystic 1 (2%)
Hepatocyte, Basophilic Focus 1 (2%) 2 (4%) 1 (2%)
Hepatocyte, Cytomegaly 1 (2%)
Hepatocyte, Depletion Glycogen, Diffuse 2 (4%) 1 (2%) 2 (4%) 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 2
NTP Experiment-Test: 93023-07 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 2,4-HEXADIENAL Date: 02/15/01
Route: GAVAGE Time: 11:07:14
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 30 60 120
MG/KG/DY MG/KG/DY MG/KG/DY MG/KG/DY
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Hepatocyte, Eosinophilic Focus 1 (2%) 1 (2%)
Hepatocyte, Karyomegaly 1 (2%)
Hepatocyte, Mixed Cell Focus 1 (2%) 1 (2%) 1 (2%)
Hepatocyte, Necrosis, Focal 1 (2%) 3 (6%) 2 (4%)
Hepatocyte, Vacuolization Cytoplasmic,
Diffuse 5 (10%) 2 (4%) 2 (4%) 5 (10%)
Hepatocyte, Vacuolization Cytoplasmic, Focal 2 (4%) 1 (2%)
Hepatocyte, Periportal, Depletion Glycogen 1 (2%)
Hepatocyte, Periportal, Vacuolization
Cytoplasmic 8 (16%) 5 (10%) 3 (6%) 1 (2%)
Hepatocyte, Centrilobular, Necrosis 1 (2%) 2 (4%)
Hepatocyte, Centrilobular, Vacuolization
Cytoplasmic 2 (4%) 1 (2%)
Hepatocyte, Midzonal, Vacuolization
Cytoplasmic 12 (24%) 7 (14%) 3 (6%) 6 (12%)
Oval Cell, Hyperplasia 1 (2%)
Portal, Infiltration Cellular, Focal,
Lymphocyte 1 (2%)
Serosa, Hyperplasia, Focal, Lymphoid 1 (2%)
Mesentery (31) (30) (37) (25)
Angiectasis 1 (3%) 2 (7%)
Angiectasis, Focal 1 (3%) 1 (4%)
Congestion, Focal 1 (3%)
Fibrosis, Focal 1 (3%)
Hemorrhage 1 (3%) 1 (3%)
Inflammation, Chronic 1 (3%)
Artery, Mineralization 2 (6%)
Fat, Necrosis 29 (94%) 28 (93%) 34 (92%) 22 (88%)
Lymphatic, Angiectasis 1 (3%) 3 (8%)
Pancreas (47) (46) (50) (47)
Infiltration Cellular, Focal, Mixed Cell 1 (2%)
Necrosis, Focal 1 (2%)
Acinus, Atrophy, Focal 1 (2%) 1 (2%)
Duct, Cyst 1 (2%) 1 (2%)
Salivary Glands (49) (48) (48) (50)
Hyperplasia, Lymphoid 2 (4%)
Submandibular Gland, Vacuolization
Cytoplasmic 1 (2%)
Stomach, Forestomach (48) (48) (50) (48)
Diverticulum 1 (2%)
Erosion 1 (2%) 1 (2%)
Erosion, Focal 1 (2%)
Inflammation, Focal 2 (4%) 2 (4%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 3
NTP Experiment-Test: 93023-07 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 2,4-HEXADIENAL Date: 02/15/01
Route: GAVAGE Time: 11:07:14
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 30 60 120
MG/KG/DY MG/KG/DY MG/KG/DY MG/KG/DY
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Ulcer 1 (2%) 1 (2%) 2 (4%)
Epithelium, Hyperplasia, Squamous 4 (8%) 8 (17%) 12 (24%) 31 (65%)
Epithelium, Ulcer, Focal 1 (2%)
Stomach, Glandular (45) (45) (48) (46)
Glands, Degeneration, Cystic, Focal 1 (2%) 1 (2%)
Muscularis, Mineralization 1 (2%)
Tooth (1) (2)
Peridontal Tissue, Inflammation, Chronic 2 (100%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Blood Vessel (2) (1)
Aorta, Mineralization 2 (100%)
Pulmonary Artery, Mineralization 2 (100%)
Pulmonary Vein, Mineralization 2 (100%)
Heart (49) (49) (50) (50)
Infiltration Cellular, Mixed Cell 1 (2%)
Mineralization, Focal 2 (4%) 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (50) (48) (50) (50)
Accessory Adrenal Cortical Nodule 2 (4%) 2 (4%) 3 (6%)
Angiectasis 1 (2%) 1 (2%)
Cyst 2 (4%)
Cytoplasmic Alteration, Focal 1 (2%)
Degeneration, Cystic, Focal 1 (2%)
Infiltration Cellular, Mixed Cell 1 (2%)
Subcapsular, Hyperplasia, Focal 1 (2%)
Adrenal Medulla (50) (48) (49) (49)
Hyperplasia 1 (2%)
Parathyroid Gland (45) (46) (49) (47)
Cyst 1 (2%) 1 (2%) 2 (4%)
Cyst, Multiple 1 (2%)
Hyperplasia, Cystic 1 (2%)
Pituitary Gland (46) (48) (47) (50)
Angiectasis 3 (7%) 1 (2%) 1 (2%)
Pars Distalis, Angiectasis 1 (2%)
Pars Distalis, Cyst, Multiple 1 (2%)
Pars Distalis, Cytoplasmic Alteration, Focal 6 (13%) 3 (6%) 2 (4%) 4 (8%)
Pars Distalis, Degeneration, Cystic, Focal 2 (4%) 2 (4%) 4 (9%) 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 4
NTP Experiment-Test: 93023-07 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 2,4-HEXADIENAL Date: 02/15/01
Route: GAVAGE Time: 11:07:14
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 30 60 120
MG/KG/DY MG/KG/DY MG/KG/DY MG/KG/DY
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM - CONT
Pars Distalis, Hemorrhage, Focal 3 (6%) 1 (2%)
Pars Distalis, Hyperplasia 1 (2%)
Pars Distalis, Hyperplasia, Focal 2 (4%) 5 (10%) 6 (13%) 2 (4%)
Rathke's Cleft, Hyperplasia, Cystic 1 (2%)
Thyroid Gland (49) (46) (49) (50)
Atrophy, Focal 1 (2%)
Degeneration, Cystic, Focal 7 (14%) 14 (30%) 9 (18%) 11 (22%)
Ectopic Thymus 2 (4%)
Inflammation, Chronic 1 (2%)
Inflammation, Chronic, Focal 1 (2%)
Follicle, Cyst 1 (2%) 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
Tissue NOS (1) (1) (2) (1)
Abdominal, Abscess 1 (100%)
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Clitoral Gland (47) (45) (45) (50)
Angiectasis 1 (2%)
Degeneration, Cystic 2 (4%) 2 (4%) 1 (2%)
Inflammation, Chronic 1 (2%)
Ovary (49) (47) (48) (48)
Angiectasis 1 (2%) 1 (2%) 1 (2%)
Cyst 13 (27%) 11 (23%) 11 (23%) 10 (21%)
Cyst, Multiple 1 (2%) 1 (2%)
Hemorrhage 1 (2%)
Hyperplasia, Cystic 1 (2%)
Hyperplasia, Tubular 1 (2%)
Inflammation, Suppurative 1 (2%)
Thrombosis 1 (2%) 1 (2%)
Bilateral, Cyst 1 (2%) 1 (2%)
Bilateral, Follicle, Cyst 1 (2%)
Bilateral, Follicle, Hemorrhage 1 (2%)
Periovarian Tissue, Mineralization 1 (2%)
Uterus (50) (49) (50) (50)
Angiectasis 1 (2%) 1 (2%) 2 (4%)
Congestion 1 (2%)
Cyst 1 (2%) 2 (4%) 1 (2%)
Cyst, Multiple 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 5
NTP Experiment-Test: 93023-07 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 2,4-HEXADIENAL Date: 02/15/01
Route: GAVAGE Time: 11:07:14
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 30 60 120
MG/KG/DY MG/KG/DY MG/KG/DY MG/KG/DY
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM - CONT
Edema 1 (2%)
Hemorrhage 1 (2%)
Hydrometra 33 (66%) 35 (71%) 40 (80%) 39 (78%)
Inflammation, Chronic 1 (2%)
Endometrium, Hyperplasia, Cystic 39 (78%) 39 (80%) 42 (84%) 40 (80%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (50) (47) (50) (48)
Hyperplasia, Megakaryocyte 1 (2%)
Myeloid Cell, Hyperplasia 3 (6%) 5 (11%) 4 (8%) 5 (10%)
Lymph Node (8) (9) (9) (7)
Iliac, Hyperplasia, Plasma Cell 1 (13%) 1 (11%) 1 (14%)
Iliac, Pigmentation 1 (13%)
Inguinal, Hyperplasia, Lymphoid 1 (13%) 2 (22%)
Inguinal, Hyperplasia, Plasma Cell 1 (13%) 1 (11%)
Inguinal, Infiltration Cellular, Mixed Cell 1 (14%)
Mediastinal, Hemorrhage 1 (14%)
Mediastinal, Hyperplasia, Lymphoid 1 (13%) 2 (22%) 2 (22%)
Mediastinal, Hyperplasia, Plasma Cell 1 (13%) 1 (14%)
Renal, Hyperplasia, Plasma Cell 1 (14%)
Renal, Infiltration Cellular, Mixed Cell 1 (14%)
Lymph Node, Mandibular (47) (47) (48) (49)
Congestion 1 (2%)
Hyperplasia, Lymphoid 1 (2%)
Hyperplasia, Plasma Cell 1 (2%)
Lymph Node, Mesenteric (47) (46) (49) (49)
Angiectasis 2 (4%)
Atypia Cellular 1 (2%)
Hyperplasia, Cystic 1 (2%)
Hyperplasia, Histiocytic 1 (2%) 2 (4%)
Hyperplasia, Lymphoid 1 (2%) 2 (4%)
Hyperplasia, Plasma Cell 3 (6%) 2 (4%)
Spleen (48) (47) (50) (49)
Accessory Spleen 1 (2%)
Angiectasis 1 (2%) 1 (2%)
Congestion 1 (2%) 3 (6%) 2 (4%)
Depletion Cellular 2 (4%)
Hematopoietic Cell Proliferation 20 (42%) 29 (62%) 29 (58%) 17 (35%)
Hemorrhage 1 (2%) 1 (2%) 1 (2%)
Hyperplasia, Histiocytic 1 (2%)
Hyperplasia, Lymphoid 8 (17%) 9 (19%) 7 (14%) 7 (14%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 6
NTP Experiment-Test: 93023-07 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 2,4-HEXADIENAL Date: 02/15/01
Route: GAVAGE Time: 11:07:14
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 30 60 120
MG/KG/DY MG/KG/DY MG/KG/DY MG/KG/DY
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM - CONT
Necrosis, Focal 1 (2%) 2 (4%)
Pigmentation, Focal 1 (2%)
Thrombosis 1 (2%)
Thymus (44) (46) (48) (46)
Atrophy 1 (2%)
Cyst 1 (2%) 3 (7%)
Hemorrhage 1 (2%)
Hyperplasia, Lymphoid 1 (2%) 1 (2%) 2 (4%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Mammary Gland (48) (49) (50) (50)
Ectasia 2 (4%) 1 (2%) 2 (4%) 1 (2%)
Hyperplasia 2 (4%) 3 (6%)
Metaplasia, Focal, Squamous 1 (2%)
Skin (50) (49) (50) (50)
Ulcer 1 (2%)
Hair Follicle, Atrophy, Focal 1 (2%)
Subcutaneous Tissue, Fibrosis, Focal 1 (2%)
Subcutaneous Tissue, Hemorrhage, Focal 1 (2%) 1 (2%)
Subcutaneous Tissue, Infiltration Cellular,
Focal, Mixed Cell 1 (2%)
Subcutaneous Tissue, Inflammation, Chronic 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Skeletal Muscle (6) (4) (5) (2)
Fibrosis 1 (20%)
Inflammation, Chronic 1 (17%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (50) (48) (50) (50)
Compression, Focal 2 (4%) 3 (6%) 2 (4%) 1 (2%)
Hemorrhage, Focal 1 (2%) 1 (2%)
Ventricle, Hydrocephalus 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
a Number of animals examined microscopically at site and number of animals with lesion
Page 7
NTP Experiment-Test: 93023-07 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 2,4-HEXADIENAL Date: 02/15/01
Route: GAVAGE Time: 11:07:14
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 30 60 120
MG/KG/DY MG/KG/DY MG/KG/DY MG/KG/DY
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM - CONT
Lung (49) (49) (50) (50)
Congestion 1 (2%) 2 (4%) 2 (4%) 2 (4%)
Fibrosis, Focal 1 (2%)
Hemorrhage 1 (2%) 2 (4%) 2 (4%)
Hemorrhage, Focal 1 (2%)
Hyperplasia, Histiocytic 2 (4%) 1 (2%)
Hyperplasia, Lymphoid 1 (2%) 3 (6%) 2 (4%)
Infiltration Cellular, Focal, Mixed Cell 1 (2%)
Infiltration Cellular, Mixed Cell 1 (2%)
Inflammation, Suppurative 1 (2%)
Thrombosis 1 (2%)
Alveolar Epithelium, Hyperplasia 1 (2%)
Artery, Mineralization 1 (2%)
Vein, Mineralization 1 (2%)
Nose (50) (48) (50) (50)
Hemorrhage 1 (2%)
Inflammation, Suppurative 18 (36%) 22 (46%) 23 (46%) 16 (32%)
Mucosa, Glands, Dilatation, Focal 5 (10%) 2 (4%) 6 (12%)
Nasolacrimal Duct, Cyst 1 (2%)
Nasolacrimal Duct, Inflammation 1 (2%) 1 (2%) 1 (2%) 1 (2%)
Nasolacrimal Duct, Mineralization 1 (2%)
Olfactory Epithelium, Cytoplasmic Alteration 28 (56%) 23 (48%) 34 (68%) 28 (56%)
Respiratory Epithelium, Cytoplasmic
Alteration 50 (100%) 47 (98%) 49 (98%) 48 (96%)
Respiratory Epithelium, Necrosis 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Eye (2) (3) (2) (2)
Atrophy 2 (67%) 2 (100%)
Cornea, Hyperplasia, Focal, Squamous 1 (50%) 1 (33%)
Cornea, Inflammation, Focal 1 (50%) 1 (33%)
Harderian Gland (8) (6) (4) (1)
Hyperplasia, Focal 1 (13%)
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (50) (47) (49) (48)
Atrophy 1 (2%)
Congestion 1 (2%) 1 (2%)
Cyst 3 (6%) 4 (9%) 1 (2%) 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 8
NTP Experiment-Test: 93023-07 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 2,4-HEXADIENAL Date: 02/15/01
Route: GAVAGE Time: 11:07:14
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 30 60 120
MG/KG/DY MG/KG/DY MG/KG/DY MG/KG/DY
____________________________________________________________________________________________________________________________________
URINARY SYSTEM - CONT
Cyst, Multiple 1 (2%)
Hydronephrosis 1 (2%)
Hyperplasia, Lymphoid 1 (2%) 1 (2%) 1 (2%) 1 (2%)
Infarct 3 (6%) 1 (2%) 5 (10%) 4 (8%)
Infarct, Focal 1 (2%)
Inflammation, Chronic, Focal 1 (2%)
Metaplasia, Focal, Osseous 2 (4%) 1 (2%) 1 (2%)
Nephropathy 19 (38%) 15 (32%) 17 (35%) 22 (46%)
Artery, Mineralization 2 (4%)
Papilla, Mineralization, Focal 1 (2%)
Papilla, Necrosis 4 (8%) 1 (2%)
Renal Tubule, Accumulation, Hyaline Droplet 3 (6%) 2 (4%) 1 (2%)
Renal Tubule, Accumulation, Hyaline Droplet,
Focal 1 (2%)
Renal Tubule, Necrosis 1 (2%)
Renal Tubule, Pigmentation 1 (2%) 2 (4%)
Urinary Bladder (48) (47) (48) (48)
Transitional Epithelium, Hyperplasia 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 9
NTP Experiment-Test: 93023-07 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 2,4-HEXADIENAL Date: 02/15/01
Route: GAVAGE Time: 11:07:14
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 30 60 120
MG/KG/DY MG/KG/DY MG/KG/DY MG/KG/DY
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 50 50 50 50
Early Deaths
Moribund Sacrifice 3 3 3 8
Natural Death 3 8 3 3
Survivors
Terminal Sacrifice 44 39 44 38
Natural Death 1
Animals Examined Microscopically 50 50 50 50
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Gallbladder (48) (47) (45) (46)
Cyst 2 (4%) 2 (4%) 1 (2%)
Cyst, Multiple 1 (2%) 1 (2%)
Infiltration Cellular, Polymorphonuclear 1 (2%)
Epithelium, Degeneration, Hyaline 1 (2%) 2 (4%)
Epithelium, Hyperplasia, Adenomatous 1 (2%) 1 (2%)
Intestine Large, Cecum (47) (45) (48) (48)
Serosa, Fibrosis, Focal 1 (2%)
Intestine Small, Duodenum (47) (47) (48) (49)
Epithelium, Cyst 1 (2%)
Intestine Small, Jejunum (47) (45) (49) (48)
Inflammation, Chronic Active 1 (2%)
Peyer's Patch, Hyperplasia, Histiocytic 1 (2%)
Peyer's Patch, Hyperplasia, Lymphoid 1 (2%)
Intestine Small, Ileum (47) (45) (47) (48)
Infiltration Cellular, Mixed Cell 1 (2%)
Serosa, Fibrosis, Focal 1 (2%)
Liver (50) (50) (50) (50)
Angiectasis 2 (4%)
Angiectasis, Focal 1 (2%)
Clear Cell Focus 1 (2%)
Congestion 1 (2%)
Congestion, Focal 1 (2%) 3 (6%) 1 (2%) 3 (6%)
Eosinophilic Focus 1 (2%)
Eosinophilic Focus, Multiple 1 (2%)
Erythrophagocytosis 1 (2%)
Hematopoietic Cell Proliferation 1 (2%)
Hemorrhage, Focal 2 (4%) 1 (2%) 2 (4%)
Hyperplasia, Focal, Histiocytic 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 10
NTP Experiment-Test: 93023-07 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 2,4-HEXADIENAL Date: 02/15/01
Route: GAVAGE Time: 11:07:14
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 30 60 120
MG/KG/DY MG/KG/DY MG/KG/DY MG/KG/DY
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Hyperplasia, Focal, Lymphoid 1 (2%)
Hyperplasia, Histiocytic 1 (2%)
Hyperplasia, Lymphoid 1 (2%)
Infarct 2 (4%)
Infiltration Cellular, Lymphocyte 1 (2%)
Infiltration Cellular, Mixed Cell 31 (62%) 27 (54%) 30 (60%) 31 (62%)
Inflammation, Chronic 1 (2%) 1 (2%)
Mixed Cell Focus 1 (2%) 1 (2%) 3 (6%)
Necrosis, Focal 2 (4%) 1 (2%)
Pigmentation, Focal 1 (2%)
Bile Duct, Hyperplasia 1 (2%)
Hepatocyte, Basophilic Focus 2 (4%) 1 (2%) 1 (2%)
Hepatocyte, Cytomegaly 2 (4%) 2 (4%) 2 (4%)
Hepatocyte, Eosinophilic Focus 3 (6%) 5 (10%) 2 (4%) 4 (8%)
Hepatocyte, Eosinophilic Focus, Multiple 1 (2%)
Hepatocyte, Karyomegaly 2 (4%) 2 (4%)
Hepatocyte, Mixed Cell Focus 1 (2%) 6 (12%) 9 (18%) 5 (10%)
Hepatocyte, Mixed Cell Focus, Multiple 1 (2%) 3 (6%) 3 (6%)
Hepatocyte, Necrosis, Focal 1 (2%) 1 (2%) 2 (4%) 2 (4%)
Hepatocyte, Vacuolization Cytoplasmic,
Diffuse 6 (12%) 1 (2%) 1 (2%) 4 (8%)
Hepatocyte, Vacuolization Cytoplasmic, Focal 13 (26%) 15 (30%) 16 (32%) 12 (24%)
Hepatocyte, Periportal, Vacuolization
Cytoplasmic 3 (6%) 3 (6%) 2 (4%) 2 (4%)
Hepatocyte, Centrilobular, Depletion Glycogen 1 (2%)
Hepatocyte, Centrilobular, Necrosis 1 (2%)
Hepatocyte, Midzonal, Vacuolization
Cytoplasmic 17 (34%) 9 (18%) 20 (40%) 10 (20%)
Oval Cell, Hyperplasia 1 (2%) 1 (2%)
Mesentery (25) (28) (33) (32)
Fibrosis, Focal 1 (4%) 1 (3%)
Hemorrhage 1 (4%) 1 (3%) 1 (3%)
Hemorrhage, Focal 1 (4%)
Inflammation, Chronic 1 (4%) 4 (14%) 2 (6%)
Fat, Necrosis 24 (96%) 27 (96%) 32 (97%) 31 (97%)
Pancreas (50) (50) (50) (50)
Necrosis, Focal 1 (2%) 1 (2%)
Acinus, Atrophy, Diffuse 1 (2%)
Acinus, Atrophy, Focal 1 (2%) 1 (2%) 1 (2%)
Acinus, Cytoplasmic Alteration 1 (2%)
Duct, Cyst 1 (2%) 1 (2%)
Duct, Inflammation, Chronic, Focal 1 (2%)
Stomach, Forestomach (50) (50) (48) (50)
a Number of animals examined microscopically at site and number of animals with lesion
Page 11
NTP Experiment-Test: 93023-07 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 2,4-HEXADIENAL Date: 02/15/01
Route: GAVAGE Time: 11:07:14
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 30 60 120
MG/KG/DY MG/KG/DY MG/KG/DY MG/KG/DY
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Inflammation, Focal 3 (6%) 1 (2%) 3 (6%) 6 (12%)
Ulcer 2 (4%) 3 (6%) 10 (20%)
Ulcer, Focal 1 (2%)
Epithelium, Cyst 1 (2%)
Epithelium, Hyperplasia, Squamous 14 (28%) 7 (14%) 9 (19%) 26 (52%)
Stomach, Glandular (50) (48) (48) (50)
Erosion 1 (2%)
Hyperplasia 1 (2%)
Mineralization 1 (2%)
Glands, Degeneration, Cystic, Focal 3 (6%)
Tooth (19) (21) (16) (19)
Malformation 11 (58%) 13 (62%) 10 (63%) 12 (63%)
Peridontal Tissue, Inflammation, Chronic 10 (53%) 9 (43%) 8 (50%) 10 (53%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (50) (50) (50) (50)
Infiltration Cellular, Mixed Cell 2 (4%)
Inflammation, Chronic, Focal 1 (2%) 2 (4%)
Mineralization, Focal 1 (2%)
Valve, Hemorrhage, Focal 1 (2%)
Valve, Inflammation, Chronic, Focal 1 (2%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (50) (50) (50) (50)
Accessory Adrenal Cortical Nodule 1 (2%) 1 (2%) 5 (10%)
Cytoplasmic Alteration, Focal 7 (14%) 2 (4%) 3 (6%) 5 (10%)
Hypertrophy, Focal 1 (2%) 1 (2%)
Bilateral, Accessory Adrenal Cortical Nodule 1 (2%)
Subcapsular, Hyperplasia, Focal 11 (22%) 7 (14%) 8 (16%) 7 (14%)
Adrenal Medulla (49) (50) (50) (50)
Angiectasis 1 (2%)
Hyperplasia 1 (2%) 1 (2%) 1 (2%)
Islets, Pancreatic (50) (50) (50) (50)
Hyperplasia 1 (2%) 1 (2%) 2 (4%)
Parathyroid Gland (47) (48) (49) (46)
Cyst 1 (2%) 2 (4%) 1 (2%)
Pituitary Gland (48) (47) (48) (49)
Pars Distalis, Cyst 3 (6%) 2 (4%) 3 (6%) 3 (6%)
Pars Distalis, Cyst, Multiple 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 12
NTP Experiment-Test: 93023-07 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 2,4-HEXADIENAL Date: 02/15/01
Route: GAVAGE Time: 11:07:14
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 30 60 120
MG/KG/DY MG/KG/DY MG/KG/DY MG/KG/DY
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM - CONT
Pars Distalis, Cytoplasmic Alteration, Focal 1 (2%)
Pars Distalis, Degeneration, Cystic, Focal 1 (2%)
Pars Distalis, Hyperplasia, Focal 2 (4%) 1 (2%)
Thyroid Gland (50) (50) (50) (50)
Degeneration, Cystic, Focal 7 (14%) 5 (10%) 5 (10%) 6 (12%)
Follicle, Cyst 1 (2%) 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
Peritoneum (1)
Inflammation, Focal 1 (100%)
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Coagulating Gland (1) (2) (1) (1)
Inflammation, Chronic 1 (100%)
Epididymis (50) (50) (50) (50)
Hemorrhage 1 (2%)
Inflammation, Chronic 1 (2%) 1 (2%)
Bilateral, Hemorrhage 1 (2%)
Preputial Gland (50) (50) (50) (50)
Degeneration, Cystic 16 (32%) 17 (34%) 9 (18%) 21 (42%)
Inflammation, Chronic 1 (2%) 1 (2%) 1 (2%) 1 (2%)
Metaplasia, Squamous 1 (2%)
Prostate (49) (50) (50) (50)
Inflammation, Chronic 1 (2%) 1 (2%)
Epithelium, Hyperplasia, Focal 1 (2%)
Seminal Vesicle (50) (50) (50) (50)
Dilatation 1 (2%) 2 (4%)
Inflammation, Chronic 1 (2%) 2 (4%) 1 (2%)
Bilateral, Dilatation 1 (2%)
Testes (50) (50) (50) (50)
Hemorrhage 1 (2%)
Mineralization 1 (2%)
Interstitial Cell, Hyperplasia 1 (2%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (50) (50) (50) (50)
Fibrosis, Focal 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 13
NTP Experiment-Test: 93023-07 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 2,4-HEXADIENAL Date: 02/15/01
Route: GAVAGE Time: 11:07:14
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 30 60 120
MG/KG/DY MG/KG/DY MG/KG/DY MG/KG/DY
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM - CONT
Thrombosis, Chronic 1 (2%)
Myeloid Cell, Hyperplasia 1 (2%) 1 (2%) 2 (4%) 3 (6%)
Lymph Node (1) (4) (2) (7)
Inguinal, Hyperplasia, Lymphoid 1 (25%)
Inguinal, Hyperplasia, Plasma Cell 1 (14%)
Mediastinal, Congestion 1 (25%)
Mediastinal, Hyperplasia, Lymphoid 1 (100%)
Mediastinal, Hyperplasia, Plasma Cell 1 (50%) 3 (43%)
Mediastinal, Infiltration Cellular, Mixed
Cell 1 (14%)
Lymph Node, Mandibular (48) (48) (49) (49)
Hyperplasia, Histiocytic 1 (2%)
Hyperplasia, Lymphoid 1 (2%) 3 (6%) 1 (2%) 1 (2%)
Hyperplasia, Plasma Cell 2 (4%) 1 (2%) 1 (2%) 3 (6%)
Pigmentation 1 (2%)
Lymph Node, Mesenteric (50) (49) (47) (50)
Congestion 1 (2%)
Hemorrhage 1 (2%) 2 (4%)
Hyperplasia, Histiocytic 1 (2%)
Hyperplasia, Lymphoid 1 (2%)
Hyperplasia, Plasma Cell 6 (12%) 2 (4%) 2 (4%)
Infiltration Cellular, Polymorphonuclear 1 (2%)
Spleen (49) (50) (50) (49)
Depletion Cellular 1 (2%) 1 (2%)
Hematopoietic Cell Proliferation 14 (29%) 18 (36%) 18 (36%) 23 (47%)
Hyperplasia, Histiocytic 1 (2%)
Hyperplasia, Lymphoid 3 (6%) 3 (6%) 2 (4%)
Thymus (44) (46) (47) (42)
Angiectasis 1 (2%) 1 (2%)
Atrophy 1 (2%) 1 (2%) 1 (2%)
Cyst 10 (23%) 9 (20%) 5 (11%) 9 (21%)
Cyst, Multiple 2 (4%)
Hyperplasia, Lymphoid 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Skin (50) (50) (50) (50)
Hyperkeratosis, Focal 1 (2%)
Inflammation, Chronic, Focal 1 (2%) 1 (2%)
Ulcer 2 (4%)
Epidermis, Hyperplasia, Focal 1 (2%)
Subcutaneous Tissue, Congestion, Focal 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 14
NTP Experiment-Test: 93023-07 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 2,4-HEXADIENAL Date: 02/15/01
Route: GAVAGE Time: 11:07:14
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 30 60 120
MG/KG/DY MG/KG/DY MG/KG/DY MG/KG/DY
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM - CONT
Subcutaneous Tissue, Necrosis, Fatty, Focal 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (50) (50) (50) (50)
Cranium, Hyperostosis 1 (2%)
Skeletal Muscle (1) (2) (3)
Infiltration Cellular, Focal, Lipocyte 1 (100%) 1 (50%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (50) (50) (50) (50)
Compression, Focal 1 (2%)
Hemorrhage, Focal 1 (2%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (50) (50) (50) (50)
Congestion 1 (2%) 3 (6%) 1 (2%)
Hemorrhage 1 (2%) 2 (4%) 1 (2%)
Hyperplasia, Histiocytic 5 (10%) 3 (6%) 6 (12%) 6 (12%)
Hyperplasia, Lymphoid 2 (4%) 2 (4%) 1 (2%)
Infiltration Cellular, Eosinophil, Focal 1 (2%)
Infiltration Cellular, Focal, Mixed Cell 1 (2%)
Infiltration Cellular, Mixed Cell 2 (4%) 2 (4%) 1 (2%)
Inflammation, Chronic, Focal 1 (2%)
Metaplasia, Focal, Osseous 1 (2%)
Pigmentation, Focal 1 (2%)
Alveolar Epithelium, Hyperplasia 5 (10%) 6 (12%) 4 (8%)
Alveolar Epithelium, Hyperplasia, Focal 1 (2%)
Mediastinum, Necrosis, Fatty, Focal 1 (2%)
Nose (50) (50) (48) (50)
Foreign Body 1 (2%)
Inflammation, Suppurative 7 (14%) 11 (22%) 13 (27%) 13 (26%)
Mineralization, Focal 1 (2%)
Polyp, Inflammatory 2 (4%)
Glands, Cytoplasmic Alteration 1 (2%)
Mucosa, Glands, Dilatation, Focal 4 (8%) 2 (4%) 6 (12%)
Nasolacrimal Duct, Inflammation 2 (4%) 2 (4%) 1 (2%)
Olfactory Epithelium, Cytoplasmic Alteration 24 (48%) 19 (38%) 17 (35%) 19 (38%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 15
NTP Experiment-Test: 93023-07 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 2,4-HEXADIENAL Date: 02/15/01
Route: GAVAGE Time: 11:07:14
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 30 60 120
MG/KG/DY MG/KG/DY MG/KG/DY MG/KG/DY
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM - CONT
Respiratory Epithelium, Cytoplasmic
Alteration 48 (96%) 47 (94%) 45 (94%) 45 (90%)
Respiratory Epithelium, Metaplasia, Squamous 1 (2%)
Sinus, Glands, Fibrosis, Focal 1 (2%)
Vomeronasal Organ, Cyst 1 (2%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Eye (1) (3) (3)
Atrophy 1 (33%)
Cornea, Hyperplasia, Focal, Squamous 1 (33%) 3 (100%)
Cornea, Inflammation, Chronic 2 (67%)
Cornea, Inflammation, Focal 1 (33%) 3 (100%)
Cornea, Necrosis, Focal 2 (67%)
Iris, Synechia 1 (33%)
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (50) (48) (50) (50)
Accumulation, Hyaline Droplet 1 (2%)
Atrophy 1 (2%)
Atrophy, Focal 1 (2%) 2 (4%) 1 (2%)
Congestion 2 (4%) 1 (2%) 1 (2%)
Cyst 7 (14%) 11 (23%) 4 (8%) 5 (10%)
Cyst, Multiple 1 (2%) 1 (2%) 1 (2%)
Hydronephrosis 1 (2%)
Hyperplasia, Lymphoid 1 (2%) 1 (2%)
Infiltration Cellular, Focal, Mixed Cell 1 (2%)
Inflammation, Suppurative 1 (2%) 1 (2%)
Metaplasia, Focal, Osseous 3 (6%) 1 (2%) 1 (2%)
Nephropathy 47 (94%) 44 (92%) 48 (96%) 46 (92%)
Papilla, Necrosis 1 (2%)
Renal Tubule, Accumulation, Hyaline Droplet 1 (2%)
Renal Tubule, Dilatation, Focal 1 (2%)
Renal Tubule, Hyperplasia, Focal 1 (2%) 2 (4%) 2 (4%) 4 (8%)
Renal Tubule, Pigmentation 1 (2%) 1 (2%)
Urethra (1) (1)
Inflammation 1 (100%)
Necrosis, Focal 1 (100%)
Urinary Bladder (50) (49) (49) (50)
Inflammation, Suppurative 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 16
NTP Experiment-Test: 93023-07 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 2,4-HEXADIENAL Date: 02/15/01
Route: GAVAGE Time: 11:07:14
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 30 60 120
MG/KG/DY MG/KG/DY MG/KG/DY MG/KG/DY
____________________________________________________________________________________________________________________________________
URINARY SYSTEM - CONT
Transitional Epithelium, Hyperplasia 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 17
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