TDMS Study 93029-03 Pathology Tables
NTP Experiment-Test: 93029-03 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/18/03
Route: DOSED FEED Time: 15:01:05
Facility: National Center for Toxicological Research
Chemical CAS #: 129-73-7
Lock Date: None
Cage Range: All
Reasons For Removal: All
Removal Date Range: All
Treatment Groups: Include All
a Number of animals examined microscopically at site and number of animals with lesion
Page 1
NTP Experiment-Test: 93029-03 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/18/03
Route: DOSED FEED Time: 15:01:05
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS-NCTR FEMALE 0 PPM 91 PPM 272 PPM 543 PPM
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially in Study 48 48 48 48
Early Deaths
Moribund 12 11 12 10
Natural Death 3 1 1 4
Survivors
Moribund 1
Terminal Sacrifice 33 36 35 33
Animals Examined Microscopically 48 48 48 48
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ALIMENTARY SYSTEM
Intestine Large (48) (48) (48) (48)
Colon, Adenocarcinoma 1 (2%)
Intestine Small (48) (48) (48) (48)
Liver (48) (48) (48) (48)
Hepatocellular Adenoma 1 (2%) 3 (6%) 3 (6%)
Mesentery (8) (7) (1) (4)
Sarcoma 1 (13%)
Schwannoma Malignant, Metastatic, Uterus 1 (13%)
Pancreas (48) (48) (47) (48)
Schwannoma Malignant, Metastatic, Uterus 1 (2%)
Acinar Cell, Adenoma 1 (2%)
Salivary Glands (48) (48) (48) (48)
Sarcoma 1 (2%)
Parotid Gland, Adenoma 1 (2%)
Stomach (48) (48) (48) (48)
Tongue (48) (48) (48) (48)
Squamous Cell Carcinoma 1 (2%)
Squamous Cell Papilloma 1 (2%)
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CARDIOVASCULAR SYSTEM
Blood Vessel (48) (48) (48) (48)
Heart (48) (48) (48) (48)
Endocardium, Schwannoma Malignant 1 (2%)
Myocardium, Schwannoma Malignant 1 (2%)
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Page 2
NTP Experiment-Test: 93029-03 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/18/03
Route: DOSED FEED Time: 15:01:05
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS-NCTR FEMALE 0 PPM 91 PPM 272 PPM 543 PPM
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Gland (47) (48) (47) (48)
Medulla, Pheochromocytoma Malignant 1 (2%)
Medulla, Pheochromocytoma Benign 1 (2%)
Islets, Pancreatic (48) (48) (47) (48)
Pituitary Gland (47) (47) (45) (46)
Pars Distalis, Adenoma 26 (55%) 23 (49%) 17 (38%) 20 (43%)
Thyroid Gland (46) (46) (47) (48)
Bilateral, C-Cell, Adenoma 1 (2%) 1 (2%)
Bilateral, C-Cell, Carcinoma 1 (2%)
C-Cell, Adenoma 4 (9%) 7 (15%) 4 (9%) 4 (8%)
C-Cell, Carcinoma 1 (2%)
Follicular Cell, Adenoma 1 (2%)
Follicular Cell, Carcinoma 1 (2%) 2 (4%)
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GENERAL BODY SYSTEM
Tissue NOS (1) (1)
Schwannoma Malignant, Metastatic, Uterus 1 (100%)
Abdominal, Lipoma 1 (100%)
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GENITAL SYSTEM
Clitoral Gland (47) (47) (46) (47)
Adenoma 7 (15%) 8 (17%) 3 (7%) 6 (13%)
Carcinoma 2 (4%) 1 (2%) 2 (4%) 2 (4%)
Ovary (48) (48) (48) (48)
Oviduct (1)
Leiomyoma 1 (100%)
Uterus (48) (48) (48) (48)
Leiomyoma 1 (2%) 1 (2%)
Polyp Stromal 9 (19%) 10 (21%) 16 (33%) 16 (33%)
Sarcoma Stromal 1 (2%) 1 (2%)
Schwannoma Malignant 1 (2%) 1 (2%)
Bilateral, Polyp Stromal 1 (2%) 2 (4%) 3 (6%) 1 (2%)
Cervix, Squamous Cell Papilloma 1 (2%)
Endometrium, Adenoma 1 (2%) 1 (2%)
Endometrium, Carcinoma 1 (2%)
Vagina (48) (48) (48) (48)
Sarcoma 1 (2%)
Schwannoma Malignant 1 (2%)
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NTP Experiment-Test: 93029-03 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/18/03
Route: DOSED FEED Time: 15:01:05
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS-NCTR FEMALE 0 PPM 91 PPM 272 PPM 543 PPM
____________________________________________________________________________________________________________________________________
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Lymph Node (48) (48) (48) (48)
Mesenteric, Schwannoma Malignant, Metastatic,
Uterus 1 (2%)
Spleen (48) (48) (48) (48)
Thymus (41) (47) (44) (42)
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INTEGUMENTARY SYSTEM
Mammary Gland (48) (46) (48) (48)
Adenoma 1 (2%) 1 (2%) 2 (4%)
Carcinoma 1 (2%) 2 (4%) 2 (4%)
Fibroadenoma 20 (42%) 12 (26%) 9 (19%) 13 (27%)
Fibroadenoma, Multiple 3 (6%)
Skin (48) (48) (48) (48)
Basal Cell Adenoma 1 (2%) 1 (2%)
Basal Cell Carcinoma 1 (2%)
Fibroma 1 (2%) 1 (2%)
Fibrous Histiocytoma 1 (2%)
Keratoacanthoma 1 (2%) 1 (2%)
Sarcoma 2 (4%)
Squamous Cell Papilloma 1 (2%)
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MUSCULOSKELETAL SYSTEM
None
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NERVOUS SYSTEM
Brain (48) (48) (48) (48)
Cranial Nerve, Schwannoma Malignant 1 (2%)
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Page 4
NTP Experiment-Test: 93029-03 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/18/03
Route: DOSED FEED Time: 15:01:05
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS-NCTR FEMALE 0 PPM 91 PPM 272 PPM 543 PPM
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (48) (48) (48) (48)
Alveolar/Bronchiolar Adenoma 1 (2%) 2 (4%) 3 (6%)
Carcinoma, Metastatic, Thyroid Gland 1 (2%)
Nose (48) (48) (48) (48)
Trachea (48) (48) (48) (48)
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SPECIAL SENSES SYSTEM
Ear (1)
Pinna, Neural Crest Tumor 1 (100%)
Eye (48) (48) (48) (48)
Lids, Sarcoma 1 (2%)
Harderian Gland (48) (48) (48) (48)
Zymbal's Gland (46) (47) (47) (46)
Adenoma 2 (4%)
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URINARY SYSTEM
Kidney (48) (48) (48) (48)
Hemangiosarcoma 1 (2%)
Mesenchymal Tumor Benign 1 (2%)
Sarcoma 1 (2%)
Renal Tubule, Carcinoma 1 (2%)
Urinary Bladder (47) (47) (48) (48)
Transitional Epithelium, Carcinoma 1 (2%)
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SYSTEMIC LESIONS
Multiple Organs *(48) *(48) *(48) *(48)
Hemangiosarcoma 1 (2%)
Leukemia Mononuclear 17 (35%) 8 (17%) 5 (10%) 8 (17%)
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* Number of animals with any tissue examined microscopically
Page 5
NTP Experiment-Test: 93029-03 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/18/03
Route: DOSED FEED Time: 15:01:05
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS-NCTR FEMALE 0 PPM 91 PPM 272 PPM 543 PPM
____________________________________________________________________________________________________________________________________
TUMOR SUMMARY
Total Animals with Primary Neoplasms (b) 46 46 42 43
Total Primary Neoplasms 99 92 84 90
Total Animals with Benign Neoplasms 41 43 37 38
Total Benign Neoplasms 74 73 63 75
Total Animals with Malignant Neoplasms 21 16 19 15
Total Malignant Neoplasms 25 18 21 15
Total Animals with Metastatic Neoplasms 1 1
Total Metastatic Neoplasm 4 1
Total Animals with Malignant Neoplasms
Uncertain Primary Site
Total Animals with Neoplasms Uncertain-
Benign or Malignant 1
Total Uncertain Neoplasms 1
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a Number of animals examined microscopically at site and number of animals with lesion
b Primary tumors: all tumors except metastatic tumors
Page 6
NTP Experiment-Test: 93029-03 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/18/03
Route: DOSED FEED Time: 15:01:05
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS-NCTR MALE 0 PPM 91 PPM 272 PPM 543 PPM
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially in Study 48 48 48 48
Early Deaths
Moribund 20 12 11 10
Natural Death 4 5 3 7
Survivors
Natural Death 1 1
Terminal Sacrifice 23 29 34 30
Wrong Sex 1 1
Animals Examined Microscopically 48 47 48 47
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ALIMENTARY SYSTEM
Intestine Large (48) (47) (48) (47)
Colon, Adenoma 1 (2%)
Rectum, Sarcoma, Metastatic, Skin 1 (2%)
Intestine Small (47) (47) (48) (47)
Ileum, Leiomyoma 1 (2%) 1 (2%)
Jejunum, Leiomyoma 1 (2%)
Liver (48) (47) (48) (47)
Fibrous Histiocytoma, Metastatic, Skin 1 (2%)
Hepatocellular Adenoma 2 (4%) 2 (4%) 3 (6%) 2 (4%)
Histiocytic Sarcoma 1 (2%)
Sarcoma, Metastatic, Skin 1 (2%)
Mesentery (5) (3) (6) (3)
Pancreas (48) (47) (48) (47)
Sarcoma, Metastatic, Skin 1 (2%)
Acinar Cell, Adenocarcinoma 1 (2%)
Salivary Glands (48) (47) (48) (47)
Fibrous Histiocytoma 1 (2%)
Histiocytic Sarcoma 1 (2%)
Stomach (48) (47) (47) (47)
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CARDIOVASCULAR SYSTEM
Blood Vessel (48) (47) (48) (47)
Heart (48) (47) (48) (47)
Histiocytic Sarcoma 1 (2%)
Sarcoma, Metastatic, Skin 1 (2%)
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NTP Experiment-Test: 93029-03 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/18/03
Route: DOSED FEED Time: 15:01:05
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS-NCTR MALE 0 PPM 91 PPM 272 PPM 543 PPM
____________________________________________________________________________________________________________________________________
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Gland (48) (47) (48) (47)
Bilateral, Medulla, Pheochromocytoma Benign 1 (2%)
Cortex, Adenoma 1 (2%) 1 (2%) 1 (2%)
Medulla, Pheochromocytoma Malignant 2 (4%) 3 (6%)
Medulla, Pheochromocytoma Complex 1 (2%)
Medulla, Pheochromocytoma Benign 3 (6%) 5 (11%) 2 (4%) 3 (6%)
Islets, Pancreatic (48) (47) (48) (47)
Adenoma 5 (10%) 2 (4%) 1 (2%) 4 (9%)
Pituitary Gland (45) (46) (48) (45)
Craniopharyngioma 1 (2%)
Pars Distalis, Adenoma 30 (67%) 19 (41%) 21 (44%) 13 (29%)
Thyroid Gland (47) (47) (48) (46)
Fibrous Histiocytoma 1 (2%)
Bilateral, Follicular Cell, Carcinoma 1 (2%)
C-Cell, Adenoma 6 (13%) 7 (15%) 7 (15%) 4 (9%)
C-Cell, Carcinoma 1 (2%) 2 (4%)
Follicular Cell, Adenoma 2 (4%) 1 (2%)
Follicular Cell, Carcinoma 1 (2%) 1 (2%)
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GENERAL BODY SYSTEM
Tissue NOS (1) (1)
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GENITAL SYSTEM
Epididymis (48) (47) (48) (47)
Sarcoma, Metastatic, Skin 2 (4%)
Preputial Gland (48) (47) (48) (47)
Adenoma 5 (10%) 2 (4%) 7 (15%) 3 (6%)
Carcinoma 6 (13%) 4 (8%) 9 (19%)
Sarcoma, Metastatic, Skin 2 (4%)
Squamous Cell Papilloma 1 (2%)
Prostate (48) (47) (48) (47)
Testes (48) (47) (48) (47)
Bilateral, Interstitial Cell, Adenoma 22 (46%) 30 (64%) 38 (79%) 39 (83%)
Interstitial Cell, Adenoma 15 (31%) 12 (26%) 5 (10%) 6 (13%)
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Page 8
NTP Experiment-Test: 93029-03 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/18/03
Route: DOSED FEED Time: 15:01:05
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS-NCTR MALE 0 PPM 91 PPM 272 PPM 543 PPM
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (48) (47) (48) (47)
Lymph Node (48) (47) (48) (47)
Spleen (48) (47) (48) (47)
Adenocarcinoma, Metastatic, Pancreas 1 (2%)
Thymus (43) (42) (43) (42)
Fibrous Histiocytoma 1 (2%)
Histiocytic Sarcoma 1 (2%)
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INTEGUMENTARY SYSTEM
Mammary Gland (43) (41) (46) (41)
Carcinoma 1 (2%)
Fibroadenoma 1 (2%) 2 (5%) 1 (2%) 4 (10%)
Skin (48) (47) (47) (47)
Basal Cell Adenoma 1 (2%)
Basal Cell Carcinoma 1 (2%)
Fibroma 4 (8%) 1 (2%) 1 (2%) 5 (11%)
Fibrous Histiocytoma 1 (2%) 1 (2%) 1 (2%)
Keratoacanthoma 2 (4%) 3 (6%) 2 (4%)
Lipoma 3 (6%) 1 (2%)
Sarcoma 3 (6%) 4 (9%)
Squamous Cell Carcinoma 1 (2%)
Squamous Cell Papilloma 1 (2%)
Face, Squamous Cell Papilloma 1 (2%)
Sebaceous Gland, Adenoma 1 (2%)
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MUSCULOSKELETAL SYSTEM
Bone (48) (47) (48) (47)
Femur, Sarcoma, Metastatic, Skin 1 (2%)
Skeletal Muscle (48) (47) (48) (47)
Fibrous Histiocytoma, Metastatic, Skin 1 (2%)
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NERVOUS SYSTEM
Brain (48) (47) (48) (47)
Cerebrum, Astrocytoma Malignant 1 (2%)
Spinal Cord (48) (47) (48) (47)
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Page 9
NTP Experiment-Test: 93029-03 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/18/03
Route: DOSED FEED Time: 15:01:05
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS-NCTR MALE 0 PPM 91 PPM 272 PPM 543 PPM
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (48) (47) (48) (47)
Alveolar/Bronchiolar Adenoma 1 (2%) 3 (6%) 4 (9%)
Carcinoma, Metastatic, Thyroid Gland 1 (2%)
Fibrous Histiocytoma, Metastatic, Skin 1 (2%)
Histiocytic Sarcoma 1 (2%)
Sarcoma, Metastatic, Skin 1 (2%) 1 (2%)
Squamous Cell Carcinoma, Metastatic,
Preputial Gland 1 (2%)
Alveolar/Bronchiolar, Adenoma 1 (2%)
Nose (48) (47) (48) (47)
Trachea (48) (47) (48) (47)
Fibrous Histiocytoma 1 (2%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Ear (1) (2)
Pinna, Neural Crest Tumor 1 (100%) 1 (50%)
Eye (48) (47) (48) (47)
Lids, Sarcoma 1 (2%)
Harderian Gland (48) (47) (48) (47)
Zymbal's Gland (46) (43) (47) (47)
Carcinoma 1 (2%) 1 (2%)
Squamous Cell Carcinoma 1 (2%)
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (48) (47) (48) (47)
Histiocytic Sarcoma 1 (2%)
Urinary Bladder (48) (47) (48) (47)
Transitional Epithelium, Papilloma 1 (2%)
____________________________________________________________________________________________________________________________________
SYSTEMIC LESIONS
Multiple Organs *(48) *(47) *(48) *(47)
Leukemia Mononuclear 29 (60%) 16 (34%) 19 (40%) 7 (15%)
Mesothelioma Malignant 2 (4%) 1 (2%) 1 (2%)
* Number of animals with any tissue examined microscopically
Page 10
NTP Experiment-Test: 93029-03 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/18/03
Route: DOSED FEED Time: 15:01:05
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS-NCTR MALE 0 PPM 91 PPM 272 PPM 543 PPM
____________________________________________________________________________________________________________________________________
SYSTEMIC LESIONS - cont
Multiple Organs *(48) *(47) *(48) *(47)
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Page 11
NTP Experiment-Test: 93029-03 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/18/03
Route: DOSED FEED Time: 15:01:05
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS-NCTR MALE 0 PPM 91 PPM 272 PPM 543 PPM
____________________________________________________________________________________________________________________________________
TUMOR SUMMARY
Total Animals with Primary Neoplasms (b) 48 47 47 47
Total Primary Neoplasms 140 129 122 128
Total Animals with Benign Neoplasms 45 47 47 47
Total Benign Neoplasms 98 98 92 94
Total Animals with Malignant Neoplasms 34 23 25 26
Total Malignant Neoplasms 41 31 29 34
Total Animals with Metastatic Neoplasms 3 1 5
Total Metastatic Neoplasm 10 1 6
Total Animals with Malignant Neoplasms
Uncertain Primary Site
Total Animals with Neoplasms Uncertain-
Benign or Malignant 1 1
Total Uncertain Neoplasms 1 1
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
b Primary tumors: all tumors except metastatic tumors
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