TDMS Study 93029-04 Pathology Tables
NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 Facility: National Center for Toxicological Research Chemical CAS #: 129-73-7 Lock Date: None Cage Range: All Reasons For Removal: All Removal Date Range: All Treatment Groups: Include All Page 1 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 1| 2| 4| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 2| 2| 9| 1| 3| 8| 8| 9| 8| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 2| 5| 8| 8| 9| 2| 2| 5| 8| 0| 3| 3| 2| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 PPM | 0| 0| 1| 1| 2| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 7| 7| 7| 7| | 3| 7| 3| 5| 9| 0| 3| 9| 0| 1| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 5| 6| 7| 8| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Gallbladder | + A A + + A + + + + + + + + + + + + + + + + + +| Polyarteritis | | |__________________________________________________________________________| Intestine Large | + A + + + + + + + + + + + + + + + + + + + + + +| Rectum, Inflammation, Chronic | 3 | |__________________________________________________________________________| Intestine Large, Cecum | + A A + + + + + A + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Colon | + A A + + + + + A + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | + A + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small | + A A + + + + + A + + + + + + + + + + + + + + +| Ileum, Hyperplasia, Lymphoid | 2 | |__________________________________________________________________________| Intestine Small, Duodenum | + A A + + M + + A + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Ileum | + A A + + + + + A + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Jejunum | + A A + + + + + A + + + + + + + + + + + + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | X | Hematopoietic Cell Proliferation | 1 | Infiltration Cellular, Lymphocytic | 1 2 2 | Inflammation, Chronic Active, Focal | 2 1 1 2 2 1 1 2 | Mineralization, Focal | 1 | Necrosis | 2 | Tension Lipidosis | 2 1 2 1 | Vacuolization Cytoplasmic | 2 2 | Bile Duct, Cyst | 3 3 | Oval Cell, Hyperplasia | 2 | |__________________________________________________________________________| Mesentery | | Fat, Necrosis, Focal | | __________________________________________|__________________________________________________________________________| Page 2 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 1| 2| 4| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 2| 2| 9| 1| 3| 8| 8| 9| 8| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 2| 5| 8| 8| 9| 2| 2| 5| 8| 0| 3| 3| 2| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 PPM | 0| 0| 1| 1| 2| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 7| 7| 7| 7| | 3| 7| 3| 5| 9| 0| 3| 9| 0| 1| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 5| 6| 7| 8| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | |__________________________________________________________________________| Pancreas | + A + + + + + + A + + + + + + + + + + + + + + +| Cytoplasmic Alteration | 1 | Infiltration Cellular, Lymphocytic | 1 1 2 | Acinus, Atrophy | | |__________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + +| Acinus, Parotid Gland, Atrophy | 1 | Acinus, Submandibular Gland, Atrophy | 2 | Parotid Gland, Infiltration Cellular,| | Lymphocytic | 1 1 1 1 1 | Parotid Gland, Vacuolization | | Cytoplasmic | 2| Submandibular Gland, Infiltration | | Cellular, Lymphocytic | 2 1 2 2 1 2 1 2 1 2 1 2 | |__________________________________________________________________________| Stomach | + + A + + + + + + + + + + + + + + + + + + + + +| Forestomach, Hyperkeratosis, Focal | 1 | Forestomach, Infiltration Cellular, | | Lymphocytic | | Forestomach, Epithelium, Hyperplasia,| | Diffuse | | Forestomach, Epithelium, Hyperplasia,| | Focal | 2 | Glandular, Infiltration Cellular, | | Lymphocytic | 2 | Glandular, Ulcer | 2 | |__________________________________________________________________________| Stomach, Forestomach | + + A + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Stomach, Glandular | + + A M + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Tongue | + + + + + + + + + + + + + + + + + + + + + + + +| Artery, Inflammation, Chronic | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 3 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 1| 2| 4| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 2| 2| 9| 1| 3| 8| 8| 9| 8| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 2| 5| 8| 8| 9| 2| 2| 5| 8| 0| 3| 3| 2| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 PPM | 0| 0| 1| 1| 2| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 7| 7| 7| 7| | 3| 7| 3| 5| 9| 0| 3| 9| 0| 1| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 5| 6| 7| 8| __________________________________________|__________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + +| Polyarteritis | | Atrium Left, Thrombosis | 3 | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + M + + + + + + + + + + + +| Cortex, Accessory Adrenal Cortical | | Nodule | X X | Cortex, Cyst | X | Cortex, Hyperplasia, Focal | 1 | Cortex, Hypertrophy, Focal | 1 | Cortex, Subcapsular, Hyperplasia | 2 2 2 2 2 2 2 2 2 2 1 2 2 2 2 2 2 2 2| |__________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + M + + + + + + + + + + + +| |__________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + M + + + + + + + + + + + +| |__________________________________________________________________________| Islets, Pancreatic | + A + + + + + + A + + + + + + + + + + + M + + +| |__________________________________________________________________________| Parathyroid Gland | + + + + + + + + + M M + + + + + + + + + + + + +| |__________________________________________________________________________| Pituitary Gland | + M + + + + + + + + + + + + + + + + + + + + + +| Pars Distalis, Hyperplasia, Focal | 2 1 | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + +| Follicular Cell, Cyst | 2 2 2 1| _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Clitoral Gland | + A + + + + + + + + + + + + + + + + + + + + + +| Duct, Cyst | 1 2 1 2 2 2 1 2 2 2 2 2 2 2 2 2 2 2 2 1 2 2| |__________________________________________________________________________| Ovary | + M + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 4 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 1| 2| 4| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 2| 2| 9| 1| 3| 8| 8| 9| 8| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 2| 5| 8| 8| 9| 2| 2| 5| 8| 0| 3| 3| 2| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 PPM | 0| 0| 1| 1| 2| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 7| 7| 7| 7| | 3| 7| 3| 5| 9| 0| 3| 9| 0| 1| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 5| 6| 7| 8| __________________________________________|__________________________________________________________________________| GENITAL SYSTEM - cont | | Angiectasis | | Atrophy | 2 2 2 2 1 2 2 2 2 2 2 2 2 1 1 1 1 2 2| Cyst | X X X X X X X X| Hemorrhage | 2 | Thrombosis | 3 | Bilateral, Cyst | X X | |__________________________________________________________________________| Uterus | + A + + + + + + + + + + + + + + + + + + + + + +| Adenomyosis, Focal | 3 | Hyperplasia, Cystic | 2 1 4 2 1 4 2 1 2 2 4 2 3 2 4 3 3 2 3 3| |__________________________________________________________________________| Vagina | + A + + + + + + M + + + + + + + + + + + + + + +| Epithelium, Hyperplasia | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + A + + + + + + + + + + + + + + + + + + + + + +| Angiectasis, Focal | 2 | Femoral, Infarct | 2 | Myeloid Cell, Hyperplasia | 2 2 | |__________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + +| Lumbar, Cyst | | Lumbar, Hematopoietic Cell | | Proliferation | | Lumbar, Hyperplasia, Lymphoid | 3 | Mandibular, Hyperplasia, Lymphoid | 2 | Mediastinal, Hyperplasia, Lymphoid | 2 | Mesenteric, Hematopoietic Cell | | Proliferation | | Mesenteric, Hemorrhage | 1 | Mesenteric, Hyperplasia, Lymphoid | 1 3 | Renal, Hyperplasia, Lymphoid | 3 2 | |__________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Lymph Node, Mesenteric | + A + + + + + + A + + + + + + + + + + + + + + +| |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 5 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 1| 2| 4| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 2| 2| 9| 1| 3| 8| 8| 9| 8| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 2| 5| 8| 8| 9| 2| 2| 5| 8| 0| 3| 3| 2| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 PPM | 0| 0| 1| 1| 2| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 7| 7| 7| 7| | 3| 7| 3| 5| 9| 0| 3| 9| 0| 1| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 5| 6| 7| 8| __________________________________________|__________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | Spleen | + + A + + + + + A + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 3 2 4 3 4 1 2 | Hyperplasia, Lymphoid | 3 2 3 3 2 3 | Proliferation Connective Tissue, | | Focal | 1 | |__________________________________________________________________________| Thymus | + A M + + + + + + + + + + + + + + + + + + + + +| Atrophy | 2 | Cyst | X | Ectopic Parathyroid Gland | X X | Hyperplasia, Lymphoid | 1 2 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | + A + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skin | + A + + + + + + + + + + + + + + + + + + + + + +| Face, Ulcer | 3 | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + +| Femur, Fibrous Osteodystrophy | 1 1 2| Sternum, Degeneration, Cystic | | Sternum, Fibrous Osteodystrophy | 1 1 2 1 1 2 1 1 1 2| |__________________________________________________________________________| Bone, Femur | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skeletal Muscle | + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + +| Cerebrum, Mineralization | 1 1 1 1 1 1 1 | |__________________________________________________________________________| Brain, Brain Stem | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Brain, Cerebellum | + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 6 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 1| 2| 4| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 2| 2| 9| 1| 3| 8| 8| 9| 8| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 2| 5| 8| 8| 9| 2| 2| 5| 8| 0| 3| 3| 2| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 PPM | 0| 0| 1| 1| 2| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 7| 7| 7| 7| | 3| 7| 3| 5| 9| 0| 3| 9| 0| 1| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 5| 6| 7| 8| __________________________________________|__________________________________________________________________________| NERVOUS SYSTEM - cont | | |__________________________________________________________________________| Brain, Cerebrum | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Peripheral Nerve | + + + + + + + + + + + + + + + + + + + M + + + +| |__________________________________________________________________________| Spinal Cord | + + + + + + + + + + + + + + + + + + + + + + + +| Thoracic, Axon, Degeneration | 1 1 1 | |__________________________________________________________________________| Spinal Cord, Thoracic | + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + +| Hemorrhage, Focal | 2 | Infiltration Cellular, Histiocytic | | Infiltration Cellular, Lymphocytic | 2 2 2 2 2 1 3 2 | Inflammation, Chronic, Focal | | Alveolar Epithelium, Hyperplasia | | Vein, Thrombosis | 3 | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | + + + + + + + + + + + + + + + + + + + + + + + +| Bilateral, Lens, Cataract | 3 3 3 4 | Cornea, Inflammation, Chronic | 2 | Lens, Cataract | 2 3 1 2 1 | |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Lymphocytic | 1 2 | |__________________________________________________________________________| Zymbal's Gland | + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 7 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 1| 2| 4| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 2| 2| 9| 1| 3| 8| 8| 9| 8| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 2| 5| 8| 8| 9| 2| 2| 5| 8| 0| 3| 3| 2| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 PPM | 0| 0| 1| 1| 2| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 7| 7| 7| 7| | 3| 7| 3| 5| 9| 0| 3| 9| 0| 1| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 5| 6| 7| 8| __________________________________________|__________________________________________________________________________| URINARY SYSTEM - cont | | Kidney | + + + + + + + + + + + + + + + + + + + + + + + +| Glomerulosclerosis | 1 | Infarct | | Infiltration Cellular, Lymphocytic | 3 2 1 1 1 | Nephropathy | 3 | Papilla, Casts Protein | 1 | Pelvis, Dilatation | 4 3 | Renal Tubule, Accumulation, Hyaline | | Droplet | 3 | |__________________________________________________________________________| Urinary Bladder | + A + + + + + + + + + + + + + + + + + + + + + +| Inclusion Body Intracytoplasmic | 1 1 1 1 1 1 1 1 1| Infiltration Cellular, Lymphocytic | 1 3 2 1 1 1 1 2 2 1 1 1 1| __________________________________________|__________________________________________________________________________ Page 8 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 0 PPM | 7| 0| 0| 1| 1| 1| 1| 1| 3| 3| 4| 4| 4| 4| 4| 4| 4| 7| 7| 7| 7| 7| 7| | L | | 9| 8| 9| 0| 1| 2| 3| 4| 8| 9| 0| 1| 2| 3| 4| 5| 6| 4| 5| 6| 7| 8| 9| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + | 44 | Polyarteritis | 1 | 1 1.0| |__________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + + | 46 | Rectum, Inflammation, Chronic | | 1 3.0| |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + | 44 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + | 44 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + | 46 | |__________________________________________________________________________|____________| Intestine Small | + + + + + + + + + + + + + + + + + + + + + + + | 44 | Ileum, Hyperplasia, Lymphoid | | 1 2.0| |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + | 43 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + | 44 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + | 44 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Basophilic Focus | | 1 | Hematopoietic Cell Proliferation | 2 | 2 1.5| Infiltration Cellular, Lymphocytic | 1 2 1 1 2 | 8 1.5| Inflammation, Chronic Active, Focal | 2 1 1 1 2 1 1 | 15 1.4| Mineralization, Focal | | 1 1.0| Necrosis | | 1 2.0| Tension Lipidosis | 2 2 1 2 2 1 | 10 1.6| Vacuolization Cytoplasmic | | 2 2.0| Bile Duct, Cyst | 1 | 3 2.3| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 9 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 0 PPM | 7| 0| 0| 1| 1| 1| 1| 1| 3| 3| 4| 4| 4| 4| 4| 4| 4| 7| 7| 7| 7| 7| 7| | L | | 9| 8| 9| 0| 1| 2| 3| 4| 8| 9| 0| 1| 2| 3| 4| 5| 6| 4| 5| 6| 7| 8| 9| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | Oval Cell, Hyperplasia | | 1 2.0| |__________________________________________________________________________|____________| Mesentery | + | 1 | Fat, Necrosis, Focal | 2 | 1 2.0| |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + | 45 | Cytoplasmic Alteration | | 1 1.0| Infiltration Cellular, Lymphocytic | 1 2 1 2 | 7 1.4| Acinus, Atrophy | 1 | 1 1.0| |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Acinus, Parotid Gland, Atrophy | | 1 1.0| Acinus, Submandibular Gland, Atrophy | | 1 2.0| Parotid Gland, Infiltration Cellular,| | | Lymphocytic | 2 1 1 2 | 9 1.2| Parotid Gland, Vacuolization | | | Cytoplasmic | 2 | 2 2.0| Submandibular Gland, Infiltration | | | Cellular, Lymphocytic | 1 1 1 1 2 2 1 2 2 2 2 1 2 1 2 1 2 1 2 2 3 | 33 1.6| |__________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + | 46 | Forestomach, Hyperkeratosis, Focal | | 1 1.0| Forestomach, Infiltration Cellular, | | | Lymphocytic | 2 | 1 2.0| Forestomach, Epithelium, Hyperplasia,| | | Diffuse | 3 | 1 3.0| Forestomach, Epithelium, Hyperplasia,| | | Focal | 2 2 | 3 2.0| Glandular, Infiltration Cellular, | | | Lymphocytic | | 1 2.0| Glandular, Ulcer | | 1 2.0| |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + | 46 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 10 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 0 PPM | 7| 0| 0| 1| 1| 1| 1| 1| 3| 3| 4| 4| 4| 4| 4| 4| 4| 7| 7| 7| 7| 7| 7| | L | | 9| 8| 9| 0| 1| 2| 3| 4| 8| 9| 0| 1| 2| 3| 4| 5| 6| 4| 5| 6| 7| 8| 9| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + | 45 | |__________________________________________________________________________|____________| Tongue | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Artery, Inflammation, Chronic | 2 | 1 2.0| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Polyarteritis | 3 | 1 3.0| Atrium Left, Thrombosis | | 1 3.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + | 46 | Cortex, Accessory Adrenal Cortical | | | Nodule | X | 3 | Cortex, Cyst | | 1 | Cortex, Hyperplasia, Focal | 1 | 2 1.0| Cortex, Hypertrophy, Focal | 2 | 2 1.5| Cortex, Subcapsular, Hyperplasia | 1 2 2 2 2 2 2 2 2 2 2 1 2 2 2 2 2 2 2 2 2 2 2 | 42 1.9| |__________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + | 46 | |__________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + M + + + + + | 45 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + | 44 | |__________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + + + + M + + + + + + M M | 42 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + M + M + M + + M M + + + | 41 | Pars Distalis, Hyperplasia, Focal | 1 | 3 1.3| |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 11 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 0 PPM | 7| 0| 0| 1| 1| 1| 1| 1| 3| 3| 4| 4| 4| 4| 4| 4| 4| 7| 7| 7| 7| 7| 7| | L | | 9| 8| 9| 0| 1| 2| 3| 4| 8| 9| 0| 1| 2| 3| 4| 5| 6| 4| 5| 6| 7| 8| 9| | | _____________________________________________________________________________________________________________________|____________| ENDOCRINE SYSTEM - cont | | | Thyroid Gland | + + + + + + + + + + + + + + M + + + + + + + + | 46 | Follicular Cell, Cyst | 1 2 2 | 7 1.7| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | + + + + + + + + + + + M + + + + + + + + + + + | 45 | Duct, Cyst | 1 3 2 1 1 2 2 2 2 2 3 2 2 2 2 2 2 2 2 2 2 2 | 44 1.9| |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + | 46 | Angiectasis | 2 | 1 2.0| Atrophy | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 2 2 2 2 2 2 2 | 42 1.9| Cyst | X X X X X X | 14 | Hemorrhage | 3 1 1 | 4 1.8| Thrombosis | 3 3 | 3 3.0| Bilateral, Cyst | X X X X X | 7 | |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + | 46 | Adenomyosis, Focal | | 1 3.0| Hyperplasia, Cystic | 2 2 1 1 1 4 2 3 3 3 2 1 3 2 4 2 2 4 2 3 3 1 | 42 2.4| |__________________________________________________________________________|____________| Vagina | + + + + + + + + + + + + + + + + + + + + + + + | 45 | Epithelium, Hyperplasia | 2 | 1 2.0| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + | 46 | Angiectasis, Focal | | 1 2.0| Femoral, Infarct | | 1 2.0| Myeloid Cell, Hyperplasia | 2 3 | 4 2.3| |__________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + | 47 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 12 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 0 PPM | 7| 0| 0| 1| 1| 1| 1| 1| 3| 3| 4| 4| 4| 4| 4| 4| 4| 7| 7| 7| 7| 7| 7| | L | | 9| 8| 9| 0| 1| 2| 3| 4| 8| 9| 0| 1| 2| 3| 4| 5| 6| 4| 5| 6| 7| 8| 9| | | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | Lumbar, Cyst | X | 1 | Lumbar, Hematopoietic Cell | | | Proliferation | 3 | 1 3.0| Lumbar, Hyperplasia, Lymphoid | | 1 3.0| Mandibular, Hyperplasia, Lymphoid | | 1 2.0| Mediastinal, Hyperplasia, Lymphoid | | 1 2.0| Mesenteric, Hematopoietic Cell | | | Proliferation | 2 | 1 2.0| Mesenteric, Hemorrhage | | 1 1.0| Mesenteric, Hyperplasia, Lymphoid | 2 2 3 | 5 2.2| Renal, Hyperplasia, Lymphoid | | 2 2.5| |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | M + + + + + + + + + + + + + + + + + + + + + + | 44 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + | 45 | Hematopoietic Cell Proliferation | 1 2 3 2 | 11 2.5| Hyperplasia, Lymphoid | 1 2 2 1 2 2 2 2 | 14 2.1| Proliferation Connective Tissue, | | | Focal | | 1 1.0| |__________________________________________________________________________|____________| Thymus | + + + + + + + + + + + M + + + + + M + + + + + | 43 | Atrophy | 2 | 2 2.0| Cyst | X | 2 | Ectopic Parathyroid Gland | X X | 4 | Hyperplasia, Lymphoid | | 2 1.5| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + | 46 | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + | 46 | Face, Ulcer | | 1 3.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 13 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 0 PPM | 7| 0| 0| 1| 1| 1| 1| 1| 3| 3| 4| 4| 4| 4| 4| 4| 4| 7| 7| 7| 7| 7| 7| | L | | 9| 8| 9| 0| 1| 2| 3| 4| 8| 9| 0| 1| 2| 3| 4| 5| 6| 4| 5| 6| 7| 8| 9| | | _____________________________________________________________________________________________________________________|____________| MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Femur, Fibrous Osteodystrophy | 1 3 1 1 1 | 8 1.4| Sternum, Degeneration, Cystic | 1 | 1 1.0| Sternum, Fibrous Osteodystrophy | 1 1 1 1 1 3 1 1 1 1 1 3 2 2 1 2 | 26 1.4| |__________________________________________________________________________|____________| Bone, Femur | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Skeletal Muscle | + + + + + + + + + + + + + + + + + + + + + + + | 47 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Cerebrum, Mineralization | 1 1 1 1 1 1 1 1 | 15 1.0| |__________________________________________________________________________|____________| Brain, Brain Stem | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Brain, Cerebellum | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Brain, Cerebrum | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Peripheral Nerve | + + + + + + + + + + + + + + + + + + + + + + + | 46 | |__________________________________________________________________________|____________| Spinal Cord | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Thoracic, Axon, Degeneration | 1 1 1 1 1 1 1 1 1 | 12 1.0| |__________________________________________________________________________|____________| Spinal Cord, Thoracic | + + + + + + + + + + + + + + + + + + + + + + + | 47 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Hemorrhage, Focal | | 1 2.0| Infiltration Cellular, Histiocytic | 1 | 1 1.0| Infiltration Cellular, Lymphocytic | 1 2 1 2 2 | 13 1.8| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 14 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 0 PPM | 7| 0| 0| 1| 1| 1| 1| 1| 3| 3| 4| 4| 4| 4| 4| 4| 4| 7| 7| 7| 7| 7| 7| | L | | 9| 8| 9| 0| 1| 2| 3| 4| 8| 9| 0| 1| 2| 3| 4| 5| 6| 4| 5| 6| 7| 8| 9| | | _____________________________________________________________________________________________________________________|____________| RESPIRATORY SYSTEM - cont | | | Inflammation, Chronic, Focal | 1 | 1 1.0| Alveolar Epithelium, Hyperplasia | 1 | 1 1.0| Vein, Thrombosis | | 1 3.0| |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + | 47 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Bilateral, Lens, Cataract | | 4 3.3| Cornea, Inflammation, Chronic | | 1 2.0| Lens, Cataract | | 5 1.8| |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Infiltration Cellular, Lymphocytic | 1 1 | 4 1.3| |__________________________________________________________________________|____________| Zymbal's Gland | + + + + + + + + + + + + + + + + + + + + + + + | 47 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Glomerulosclerosis | | 1 1.0| Infarct | 1 1 | 2 1.0| Infiltration Cellular, Lymphocytic | 1 2 1 1 | 9 1.4| Nephropathy | | 1 3.0| Papilla, Casts Protein | | 1 1.0| Pelvis, Dilatation | | 2 3.5| Renal Tubule, Accumulation, Hyaline | | | Droplet | | 1 3.0| |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + | 46 | Inclusion Body Intracytoplasmic | 1 1 1 1 1 | 14 1.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 15 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 0 PPM | 7| 0| 0| 1| 1| 1| 1| 1| 3| 3| 4| 4| 4| 4| 4| 4| 4| 7| 7| 7| 7| 7| 7| | L | | 9| 8| 9| 0| 1| 2| 3| 4| 8| 9| 0| 1| 2| 3| 4| 5| 6| 4| 5| 6| 7| 8| 9| | | _____________________________________________________________________________________________________________________|____________| URINARY SYSTEM - cont | | | Infiltration Cellular, Lymphocytic | 1 1 2 2 1 1 2 1 2 1 1 1 1 1 2 2 | 29 1.4| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 16 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 4| 5| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 9| 9| 7| 9| 0| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 8| 0| 5| 0| 8| 9| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 91 PPM | 1| 2| 3| 3| 3| 3| 5| 5| 5| 5| 6| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 0| 1| 1| 1| | 6| 4| 1| 4| 6| 7| 6| 7| 8| 9| 0| 0| 1| 2| 3| 4| 5| 7| 8| 9| 0| 4| 5| 6| 7| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + M + + + + + + + + + +| Infiltration Cellular, Lymphocytic | | |__________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + + + +| Rectum, Inflammation, Chronic | 2 | |__________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Colon | + A + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + + + + + + + + +| Ileum, Hyperplasia, Lymphoid | 1 | |__________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | X X | Clear Cell Focus | | Hematopoietic Cell Proliferation | 3 3 | Infiltration Cellular, Lymphocytic | | Inflammation, Chronic Active, Focal | 1 1 1 1 2 1 2 | Tension Lipidosis | 1 1 1 1 1 1 1 1| Vacuolization Cytoplasmic | 1 | Bile Duct, Cyst | 2 | |__________________________________________________________________________| Mesentery | + + | Fat, Necrosis, Focal | 2 2 | |__________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 17 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 4| 5| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 9| 9| 7| 9| 0| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 8| 0| 5| 0| 8| 9| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 91 PPM | 1| 2| 3| 3| 3| 3| 5| 5| 5| 5| 6| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 0| 1| 1| 1| | 6| 4| 1| 4| 6| 7| 6| 7| 8| 9| 0| 0| 1| 2| 3| 4| 5| 7| 8| 9| 0| 4| 5| 6| 7| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | Cyst | | Cytoplasmic Alteration | 1 | Infiltration Cellular, Lymphocytic | 2 1 | Acinus, Atrophy | 2 2 | |__________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Acinus, Parotid Gland, Atrophy | 2 4 | Acinus, Submandibular Gland, Atrophy | 3 | Parotid Gland, Infiltration Cellular,| | Lymphocytic | 1 2 1 1 1 | Submandibular Gland, Infiltration | | Cellular, Lymphocytic | 2 1 2 1 2 2 2 2 1 1 1 1 2 1 2 2 2| |__________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Forestomach, Epithelium, Hyperplasia,| | Focal | 2 | Glandular, Infiltration Cellular, | | Lymphocytic | 2 | |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Tongue | + + + + + + + + + + + + + + + + + + + + + + + + +| Foreign Body | 1 1 | Artery, Inflammation, Chronic | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + + +| Aorta, Embolus Bacterial | 4 | |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Cardiomyopathy | | Inflammation, Acute, Focal | 2 | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 18 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 4| 5| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 9| 9| 7| 9| 0| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 8| 0| 5| 0| 8| 9| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 91 PPM | 1| 2| 3| 3| 3| 3| 5| 5| 5| 5| 6| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 0| 1| 1| 1| | 6| 4| 1| 4| 6| 7| 6| 7| 8| 9| 0| 0| 1| 2| 3| 4| 5| 7| 8| 9| 0| 4| 5| 6| 7| __________________________________________|__________________________________________________________________________| ENDOCRINE SYSTEM - cont | | Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Cortex, Accessory Adrenal Cortical | | Nodule | X | Cortex, Hypertrophy, Focal | | Cortex, Subcapsular, Hyperplasia | 2 2 1 2 2 2 1 2 2 2 2 2 2 2 2 2 2 2 2 1 2 2 2 2| Medulla, Hyperplasia, Focal | | |__________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Parathyroid Gland | + + M + + + + + + + + + M + + + + + + + + + + + +| Infiltration Cellular, Lymphocytic | 3 | |__________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Pars Distalis, Hyperplasia, Focal | | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Ultimobranchial Cyst | X | Follicular Cell, Cyst | 2 1 1 2 2 2 1 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Clitoral Gland | M + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic | | Duct, Cyst | 2 1 2 2 2 1 2 2 2 2 2 2 2 2 2 2 2 2 2 1 2 2 2 1| |__________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Abscess | | Angiectasis | | Atrophy | 2 2 2 3 1 2 2 2 2 2 2 2 1 2 2 2 3 2 1 2 2 2 1| Cyst | X X X X X X X X X X| Hemorrhage | 1 | __________________________________________|__________________________________________________________________________| Page 19 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 4| 5| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 9| 9| 7| 9| 0| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 8| 0| 5| 0| 8| 9| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 91 PPM | 1| 2| 3| 3| 3| 3| 5| 5| 5| 5| 6| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 0| 1| 1| 1| | 6| 4| 1| 4| 6| 7| 6| 7| 8| 9| 0| 0| 1| 2| 3| 4| 5| 7| 8| 9| 0| 4| 5| 6| 7| __________________________________________|__________________________________________________________________________| GENITAL SYSTEM - cont | | Bilateral, Cyst | X X | |__________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 2 2 | Hyperplasia, Cystic | 3 1 4 2 4 4 4 4 2 3 2 2 3 2 2 2 3 3 3 2 2 2 3 4| Thrombosis | 4 | Endometrium, Metaplasia | | |__________________________________________________________________________| Vagina | + + + M + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Fibrosis | 3 | Myeloid Cell, Hyperplasia | 2 | |__________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| Inguinal, Hyperplasia, Lymphoid | | Mandibular, Hyperplasia, Lymphoid | 3| Mesenteric, Hemorrhage | | Mesenteric, Hyperplasia, Lymphoid | 2 2 | |__________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + M + + + + + + + + + + + + + +| |__________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Hematopoietic Cell Proliferation | 3 4 3 3 2 2 | Hyperplasia, Lymphoid | 2 2 | Infiltration Cellular, Plasma Cell | 4 | |__________________________________________________________________________| Thymus | + + + + M + + M + + + + + + + + + + + + + + + + +| Atrophy | 3 | Cyst | X | Ectopic Parathyroid Gland | X X X | Hyperplasia, Lymphoid | 1 | __________________________________________|__________________________________________________________________________| Page 20 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 4| 5| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 9| 9| 7| 9| 0| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 8| 0| 5| 0| 8| 9| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 91 PPM | 1| 2| 3| 3| 3| 3| 5| 5| 5| 5| 6| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 0| 1| 1| 1| | 6| 4| 1| 4| 6| 7| 6| 7| 8| 9| 0| 0| 1| 2| 3| 4| 5| 7| 8| 9| 0| 4| 5| 6| 7| __________________________________________|__________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Femur, Fibrous Osteodystrophy | 1 1 | Sternum, Degeneration, Cystic | 1 1 1 | Sternum, Fibrous Osteodystrophy | 1 1 2 1 1 2 | |__________________________________________________________________________| Bone, Femur | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skeletal Muscle | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Cerebrum, Mineralization | 1 1 1 1 1 1 1 1| |__________________________________________________________________________| Brain, Brain Stem | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Brain, Cerebellum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Brain, Cerebrum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Peripheral Nerve | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Spinal Cord | + + + + + + + + + + + + + + + + + + + + + + + + +| Thoracic, Axon, Degeneration | 1 1 | |__________________________________________________________________________| Spinal Cord, Thoracic | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Crystals | | __________________________________________|__________________________________________________________________________| Page 21 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 4| 5| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 9| 9| 7| 9| 0| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 8| 0| 5| 0| 8| 9| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 91 PPM | 1| 2| 3| 3| 3| 3| 5| 5| 5| 5| 6| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 0| 1| 1| 1| | 6| 4| 1| 4| 6| 7| 6| 7| 8| 9| 0| 0| 1| 2| 3| 4| 5| 7| 8| 9| 0| 4| 5| 6| 7| __________________________________________|__________________________________________________________________________| RESPIRATORY SYSTEM - cont | | Infiltration Cellular, Histiocytic | 1 | Infiltration Cellular, Lymphocytic | 1 2 1 1 | Inflammation, Chronic, Focal | | Leukocytosis | 3 1 | Alveolar Epithelium, Hyperplasia | | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | + + + + + + + + + + + + + + + + + + + + + + + + +| Bilateral, Lens, Cataract | 3 1 2 1 | Lens, Cataract | 3 1 | |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | | Hyperplasia | 2 | Infiltration Cellular, Lymphocytic | 2 2 1 1 1 1 | Acinus, Dilatation | | |__________________________________________________________________________| Zymbal's Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | 2 | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 2 | Infiltration Cellular, Lymphocytic | 1 1 1 1 | Nephropathy | 1 1 | Glomerulus, Casts Protein | | Pelvis, Dilatation | 3 | Renal Tubule, Accumulation, Hyaline | | Droplet | 3 2 | Renal Tubule, Hyperplasia | | Renal Tubule, Hypertrophy | 1| |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 22 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 4| 5| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 9| 9| 7| 9| 0| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 8| 0| 5| 0| 8| 9| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 91 PPM | 1| 2| 3| 3| 3| 3| 5| 5| 5| 5| 6| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 0| 1| 1| 1| | 6| 4| 1| 4| 6| 7| 6| 7| 8| 9| 0| 0| 1| 2| 3| 4| 5| 7| 8| 9| 0| 4| 5| 6| 7| __________________________________________|__________________________________________________________________________| URINARY SYSTEM - cont | | Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Inclusion Body Intracytoplasmic | 1 1 1 1 1 1 2 1 1 1 1 1 1 2 1 2| Infiltration Cellular, Lymphocytic | 1 2 1 1 1 1 1 1 | __________________________________________|__________________________________________________________________________ Page 23 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 91 PPM | 1| 1| 2| 2| 4| 4| 4| 5| 5| 5| 5| 5| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | L | | 8| 9| 0| 1| 7| 8| 9| 0| 1| 2| 3| 4| 3| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Infiltration Cellular, Lymphocytic | 2 | 1 2.0| |__________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Rectum, Inflammation, Chronic | | 1 2.0| |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + A + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Intestine Small | + + + + + + + + + + + + A + + + + + + + + + + | 47 | Ileum, Hyperplasia, Lymphoid | | 1 1.0| |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + A + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + A + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + A + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Basophilic Focus | | 2 | Clear Cell Focus | X | 1 | Hematopoietic Cell Proliferation | | 2 3.0| Infiltration Cellular, Lymphocytic | 1 2 | 2 1.5| Inflammation, Chronic Active, Focal | 1 1 1 2 1 2 | 13 1.3| Tension Lipidosis | 1 1 1 2 | 12 1.1| Vacuolization Cytoplasmic | | 1 1.0| Bile Duct, Cyst | | 1 2.0| |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 24 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 91 PPM | 1| 1| 2| 2| 4| 4| 4| 5| 5| 5| 5| 5| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | L | | 8| 9| 0| 1| 7| 8| 9| 0| 1| 2| 3| 4| 3| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | Mesentery | | 2 | Fat, Necrosis, Focal | | 2 2.0| |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Cyst | 2 2 | 2 2.0| Cytoplasmic Alteration | | 1 1.0| Infiltration Cellular, Lymphocytic | 1 1 1 1 1 1 1 | 9 1.1| Acinus, Atrophy | | 2 2.0| |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Acinus, Parotid Gland, Atrophy | 2 | 3 2.7| Acinus, Submandibular Gland, Atrophy | | 1 3.0| Parotid Gland, Infiltration Cellular,| | | Lymphocytic | 1 1 1 1 | 9 1.1| Submandibular Gland, Infiltration | | | Cellular, Lymphocytic | 2 2 1 2 1 1 1 1 2 2 1 2 1 | 30 1.5| |__________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Forestomach, Epithelium, Hyperplasia,| | | Focal | 2 1 1 | 4 1.5| Glandular, Infiltration Cellular, | | | Lymphocytic | 2 | 2 2.0| |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Tongue | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Foreign Body | 1 | 3 1.0| Artery, Inflammation, Chronic | 2 2 | 2 2.0| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 25 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 91 PPM | 1| 1| 2| 2| 4| 4| 4| 5| 5| 5| 5| 5| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | L | | 8| 9| 0| 1| 7| 8| 9| 0| 1| 2| 3| 4| 3| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| | | _____________________________________________________________________________________________________________________|____________| CARDIOVASCULAR SYSTEM - cont | | | Aorta, Embolus Bacterial | | 1 4.0| |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Cardiomyopathy | 1 | 1 1.0| Inflammation, Acute, Focal | | 1 2.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Cortex, Accessory Adrenal Cortical | | | Nodule | | 1 | Cortex, Hypertrophy, Focal | 1 3 | 2 2.0| Cortex, Subcapsular, Hyperplasia | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 | 47 1.9| Medulla, Hyperplasia, Focal | 1 | 1 1.0| |__________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + + M M + M M + + + + + + | 42 | Infiltration Cellular, Lymphocytic | | 1 3.0| |__________________________________________________________________________|____________| Pituitary Gland | + + + M + + + + + + + + + + M + M + + + + + + | 45 | Pars Distalis, Hyperplasia, Focal | 1 | 1 1.0| |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Ultimobranchial Cyst | X | 2 | Follicular Cell, Cyst | 2 1 1 | 10 1.5| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 26 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 91 PPM | 1| 1| 2| 2| 4| 4| 4| 5| 5| 5| 5| 5| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | L | | 8| 9| 0| 1| 7| 8| 9| 0| 1| 2| 3| 4| 3| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| | | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + M | 46 | Inflammation, Chronic | 2 | 1 2.0| Duct, Cyst | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 | 46 1.9| |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Abscess | X | 1 | Angiectasis | 2 | 1 2.0| Atrophy | 1 2 1 1 2 1 1 2 2 2 2 2 2 2 1 2 1 1 2 1 2 1 | 45 1.7| Cyst | X X X X X X | 16 | Hemorrhage | 3 | 2 2.0| Bilateral, Cyst | X | 3 | |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Angiectasis | 2 1 | 4 1.8| Hyperplasia, Cystic | 2 2 3 3 1 1 3 2 2 3 3 2 1 4 2 3 2 3 2 4 2 2 | 46 2.6| Thrombosis | | 1 4.0| Endometrium, Metaplasia | 2 | 1 2.0| |__________________________________________________________________________|____________| Vagina | + + + + + + + + + + + + + + + + + + + + + + + | 47 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Fibrosis | | 1 3.0| Myeloid Cell, Hyperplasia | | 1 2.0| |__________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Inguinal, Hyperplasia, Lymphoid | 1 | 1 1.0| Mandibular, Hyperplasia, Lymphoid | 3 2 | 3 2.7| Mesenteric, Hemorrhage | 2 | 1 2.0| Mesenteric, Hyperplasia, Lymphoid | | 2 2.0| |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + | 47 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 27 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 91 PPM | 1| 1| 2| 2| 4| 4| 4| 5| 5| 5| 5| 5| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | L | | 8| 9| 0| 1| 7| 8| 9| 0| 1| 2| 3| 4| 3| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| | | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Angiectasis | 2 | 1 2.0| Hematopoietic Cell Proliferation | 1 3 2 | 9 2.6| Hyperplasia, Lymphoid | 1 2 | 4 1.8| Infiltration Cellular, Plasma Cell | | 1 4.0| |__________________________________________________________________________|____________| Thymus | + M + + + + + + + + + + + + + + + + + + + + + | 45 | Atrophy | 2 | 2 2.5| Cyst | | 1 | Ectopic Parathyroid Gland | X X X X | 7 | Hyperplasia, Lymphoid | 1 2 | 3 1.3| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Femur, Fibrous Osteodystrophy | | 2 1.0| Sternum, Degeneration, Cystic | | 3 1.0| Sternum, Fibrous Osteodystrophy | 1 1 1 2 1 2 1 1 2 1 2 1 2 2 1 | 21 1.4| |__________________________________________________________________________|____________| Bone, Femur | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Skeletal Muscle | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 28 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 91 PPM | 1| 1| 2| 2| 4| 4| 4| 5| 5| 5| 5| 5| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | L | | 8| 9| 0| 1| 7| 8| 9| 0| 1| 2| 3| 4| 3| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| | | _____________________________________________________________________________________________________________________|____________| NERVOUS SYSTEM - cont | | | Brain | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Cerebrum, Mineralization | 1 1 1 1 1 1 1 1 1 1 | 18 1.0| |__________________________________________________________________________|____________| Brain, Brain Stem | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Brain, Cerebellum | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Brain, Cerebrum | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Peripheral Nerve | + + + + + + + + + + + + + + + + + + + + M + + | 47 | |__________________________________________________________________________|____________| Spinal Cord | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Thoracic, Axon, Degeneration | 1 1 3 1 1 1 1 1 1 | 11 1.2| |__________________________________________________________________________|____________| Spinal Cord, Thoracic | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Crystals | 2 | 1 2.0| Infiltration Cellular, Histiocytic | 1 | 2 1.0| Infiltration Cellular, Lymphocytic | 3 3 1 | 7 1.7| Inflammation, Chronic, Focal | 1 | 1 1.0| Leukocytosis | | 2 2.0| Alveolar Epithelium, Hyperplasia | 1 | 1 1.0| |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + | 48 | |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + | 48 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Bilateral, Lens, Cataract | 3 | 5 2.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 29 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 91 PPM | 1| 1| 2| 2| 4| 4| 4| 5| 5| 5| 5| 5| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | L | | 8| 9| 0| 1| 7| 8| 9| 0| 1| 2| 3| 4| 3| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| | | _____________________________________________________________________________________________________________________|____________| SPECIAL SENSES SYSTEM - cont | | | Lens, Cataract | 2 1 | 4 1.8| |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Atrophy | 1 | 1 1.0| Hyperplasia | | 1 2.0| Infiltration Cellular, Lymphocytic | 2 2 2 | 9 1.6| Acinus, Dilatation | 2 | 1 2.0| |__________________________________________________________________________|____________| Zymbal's Gland | + + + + + + + + + + + + + + + + M + + + + + + | 47 | Atrophy | | 1 2.0| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Hematopoietic Cell Proliferation | | 1 2.0| Infiltration Cellular, Lymphocytic | 1 | 5 1.0| Nephropathy | | 2 1.0| Glomerulus, Casts Protein | 2 | 1 2.0| Pelvis, Dilatation | | 1 3.0| Renal Tubule, Accumulation, Hyaline | | | Droplet | | 2 2.5| Renal Tubule, Hyperplasia | 2 | 1 2.0| Renal Tubule, Hypertrophy | | 1 1.0| |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + | 48 | Inclusion Body Intracytoplasmic | 1 1 2 1 1 1 1 1 1 1 1 1 2 1 1 2 1 | 33 1.2| Infiltration Cellular, Lymphocytic | 2 1 1 1 1 1 1 1 1 1 1 1 1 1 | 22 1.1| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 30 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 1| 4| 5| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 9| 9| 2| 2| 2| 7| 9| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 1| | 3| 8| 6| 0| 7| 5| 0| 0| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 204 PPM | 0| 1| 1| 2| 2| 3| 3| 3| 6| 6| 6| 6| 6| 6| 6| 6| 9| 9| 9| 9| 9| 9| 0| 0| 0| | 6| 4| 7| 6| 8| 2| 5| 8| 1| 2| 3| 4| 5| 6| 7| 8| 1| 2| 3| 4| 6| 8| 5| 6| 7| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + A| |__________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + + +| Cecum, Polyarteritis | 2 | Colon, Angiectasis | 2 | Rectum, Polyarteritis | | |__________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Colon | + + + + + + + + M + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + + + + + + + +| Ileum, Hyperplasia, Lymphoid | 2 | Jejunum, Hyperplasia, Lymphoid | 3 | |__________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + +| Clear Cell Focus | | Eosinophilic Focus, Single | | Hematopoietic Cell Proliferation | 1 2 4 | Infarct | | Infiltration Cellular, Lymphocytic | 2 2 | Inflammation, Chronic Active, Focal | 1 1 1 1 1 1 1 | Tension Lipidosis | 2 2 1 | Bile Duct, Cyst | 2 | |__________________________________________________________________________| Mesentery | | Fat, Necrosis, Focal | | __________________________________________|__________________________________________________________________________| Page 31 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 1| 4| 5| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 9| 9| 2| 2| 2| 7| 9| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 1| | 3| 8| 6| 0| 7| 5| 0| 0| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 204 PPM | 0| 1| 1| 2| 2| 3| 3| 3| 6| 6| 6| 6| 6| 6| 6| 6| 9| 9| 9| 9| 9| 9| 0| 0| 0| | 6| 4| 7| 6| 8| 2| 5| 8| 1| 2| 3| 4| 5| 6| 7| 8| 1| 2| 3| 4| 6| 8| 5| 6| 7| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | |__________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + +| Cytoplasmic Alteration | 1 | Infiltration Cellular, Lymphocytic | 1 1 | Polyarteritis | 3 | Acinus, Atrophy | | |__________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + +| Acinus, Parotid Gland, Necrosis, | | Focal | 2 1 | Parotid Gland, Infiltration Cellular,| | Lymphocytic | 1 1 1 2 2 | Parotid Gland, Vacuolization | | Cytoplasmic | 2| Sublingual Gland, Infiltration | | Cellular, Lymphocytic | 1 | Submandibular Gland, Infiltration | | Cellular, Lymphocytic | 1 2 1 1 2 1 1 1 2 1 1 2 1 1 2 1 1 1 2 | |__________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + +| Forestomach, Foreign Body | | Forestomach, Inflammation, Chronic | | Active | 2 | Forestomach, Epithelium, Hyperplasia,| | Focal | 2 3 1 3 | Glandular, Infiltration Cellular, | | Lymphocytic | 1 | Glandular, Polyarteritis | 2 | |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Tongue | + + + + + + + + + + + + + + + + + + + + + + + +| Foreign Body | 1 | Artery, Inflammation, Chronic | 3 | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | __________________________________________|__________________________________________________________________________| Page 32 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 1| 4| 5| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 9| 9| 2| 2| 2| 7| 9| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 1| | 3| 8| 6| 0| 7| 5| 0| 0| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 204 PPM | 0| 1| 1| 2| 2| 3| 3| 3| 6| 6| 6| 6| 6| 6| 6| 6| 9| 9| 9| 9| 9| 9| 0| 0| 0| | 6| 4| 7| 6| 8| 2| 5| 8| 1| 2| 3| 4| 5| 6| 7| 8| 1| 2| 3| 4| 6| 8| 5| 6| 7| __________________________________________|__________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | |__________________________________________________________________________| Blood Vessel | M + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + +| Bilateral, Cortex, Hypertrophy, Focal| | Cortex, Accessory Adrenal Cortical | | Nodule | | Cortex, Hematopoietic Cell | | Proliferation | 1 | Cortex, Hyperplasia, Focal | 2 | Cortex, Hypertrophy, Focal | 3| Cortex, Polyarteritis | 3 | Cortex, Subcapsular, Hyperplasia | 1 1 2 2 3 2 2 2 2 1 2 2 2 2 2 2 2 2 2 1 2 2 2 2| |__________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Parathyroid Gland | + + + + + + + M + + + + + M + + + + + + + M + +| |__________________________________________________________________________| Pituitary Gland | + + + + + M + + + + + + M + + + + + + + + + + +| Pars Distalis, Hyperplasia, Focal | | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + +| Polyarteritis | 3 | Ultimobranchial Cyst | | Follicular Cell, Cyst | 1 1 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | __________________________________________|__________________________________________________________________________| Page 33 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 1| 4| 5| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 9| 9| 2| 2| 2| 7| 9| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 1| | 3| 8| 6| 0| 7| 5| 0| 0| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 204 PPM | 0| 1| 1| 2| 2| 3| 3| 3| 6| 6| 6| 6| 6| 6| 6| 6| 9| 9| 9| 9| 9| 9| 0| 0| 0| | 6| 4| 7| 6| 8| 2| 5| 8| 1| 2| 3| 4| 5| 6| 7| 8| 1| 2| 3| 4| 6| 8| 5| 6| 7| __________________________________________|__________________________________________________________________________| GENITAL SYSTEM - cont | | |__________________________________________________________________________| Clitoral Gland | + M + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Subacute | 2 | Duct, Cyst | 2 3 2 2 2 2 2 2 2 1 1 2 2 2 2 2 2 2 2 1| |__________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Atrophy | 1 2 2 3 2 2 2 2 1 2 2 2 2 2 2 2 2 2 2 2 2 | Cyst | X X X X X X X X| Hemorrhage | | Mineralization | 1 | Bilateral, Cyst | X | |__________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 1 | Hyperplasia, Cystic | 1 1 1 1 2 1 2 3 2 3 3 2 2 2 2 2 3 3 2 3 3 2 4 1| Polyarteritis | 2 | Thrombosis | | Stroma, Fibrosis | 3| Stroma, Hyperplasia | | |__________________________________________________________________________| Vagina | + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + +| Fibrosis | 3 | Myeloid Cell, Hyperplasia | 3 3 4 2 | |__________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + +| Mandibular, Hyperplasia, Lymphoid | 3 2 4 | Mesenteric, Hematopoietic Cell | | Proliferation | | Mesenteric, Hemorrhage | | Mesenteric, Hyperplasia, Lymphoid | 4 2 | Mesenteric, Infiltration Cellular, | | Plasma Cell | | |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 34 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 1| 4| 5| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 9| 9| 2| 2| 2| 7| 9| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 1| | 3| 8| 6| 0| 7| 5| 0| 0| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 204 PPM | 0| 1| 1| 2| 2| 3| 3| 3| 6| 6| 6| 6| 6| 6| 6| 6| 9| 9| 9| 9| 9| 9| 0| 0| 0| | 6| 4| 7| 6| 8| 2| 5| 8| 1| 2| 3| 4| 5| 6| 7| 8| 1| 2| 3| 4| 6| 8| 5| 6| 7| __________________________________________|__________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + M + + + + + + + + + + + +| |__________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 4 3 1 3 3 2 3 2 2 2 | Hyperplasia, Lymphoid | 2 3 2 | |__________________________________________________________________________| Thymus | + + + + + M + + + + + + + + + + + + + + + + + +| Atrophy | 2 | Cyst | X | Ectopic Parathyroid Gland | | Hyperplasia, Lymphoid | 2 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + +| Femur, Fibrous Osteodystrophy | 2 1 | Sternum, Degeneration, Cystic | | Sternum, Fibrous Osteodystrophy | 1 1 1 1 3 2 1 | |__________________________________________________________________________| Bone, Femur | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skeletal Muscle | + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + +| Cerebrum, Mineralization | 1 1 1 1 1 1 1 1 1 1 1 | Cerebrum, Polyarteritis | | |__________________________________________________________________________| Brain, Brain Stem | + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 35 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 1| 4| 5| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 9| 9| 2| 2| 2| 7| 9| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 1| | 3| 8| 6| 0| 7| 5| 0| 0| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 204 PPM | 0| 1| 1| 2| 2| 3| 3| 3| 6| 6| 6| 6| 6| 6| 6| 6| 9| 9| 9| 9| 9| 9| 0| 0| 0| | 6| 4| 7| 6| 8| 2| 5| 8| 1| 2| 3| 4| 5| 6| 7| 8| 1| 2| 3| 4| 6| 8| 5| 6| 7| __________________________________________|__________________________________________________________________________| NERVOUS SYSTEM - cont | | |__________________________________________________________________________| Brain, Cerebellum | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Brain, Cerebrum | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Peripheral Nerve | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Spinal Cord | + + + + + + + + + + + + + + + + + + + + + + + +| Thoracic, Axon, Degeneration | 1 1 | |__________________________________________________________________________| Spinal Cord, Thoracic | + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Lymphocytic | 2 2 3 2 1 2 2 | Alveolar Epithelium, Hyperplasia | | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | + + + + + + + + + + + + + + + + + + + + + + + +| Bilateral, Lens, Cataract | 3 2 2 | Lens, Cataract | 3 3 | |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2| Infiltration Cellular, Lymphocytic | 1 1 1 1 1 | |__________________________________________________________________________| Zymbal's Gland | + + + + + + + + + + + + M + + + + + + + + + + +| _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + +| Infarct | 1 | __________________________________________|__________________________________________________________________________| Page 36 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 1| 4| 5| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 9| 9| 2| 2| 2| 7| 9| 0| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 1| | 3| 8| 6| 0| 7| 5| 0| 0| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 204 PPM | 0| 1| 1| 2| 2| 3| 3| 3| 6| 6| 6| 6| 6| 6| 6| 6| 9| 9| 9| 9| 9| 9| 0| 0| 0| | 6| 4| 7| 6| 8| 2| 5| 8| 1| 2| 3| 4| 5| 6| 7| 8| 1| 2| 3| 4| 6| 8| 5| 6| 7| __________________________________________|__________________________________________________________________________| URINARY SYSTEM - cont | | Infiltration Cellular, Lymphocytic | 1 1 1 2 1 1 1 1 1 | Nephropathy | | Polyarteritis | 3 | Renal Tubule, Accumulation, Hyaline | | Droplet | 2 | Renal Tubule, Necrosis | 2 | Renal Tubule, Pigmentation | 2 | |__________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + +| Inclusion Body Intracytoplasmic | 2 1 2 3 2 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2| Infiltration Cellular, Lymphocytic | 1 1 1 1 2 1 1 1 | Polyarteritis | 1 | Submucosa, Edema | 3 | __________________________________________|__________________________________________________________________________ Page 37 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 204 PPM | 2| 2| 2| 2| 2| 2| 2| 5| 5| 5| 5| 5| 6| 6| 9| 9| 9| 9| 9| 9| 9| 9| 9| | L | | 2| 3| 4| 5| 6| 7| 8| 5| 6| 7| 8| 9| 0| 1| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + M + + + + + M | 44 | |__________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Cecum, Polyarteritis | | 1 2.0| Colon, Angiectasis | | 1 2.0| Rectum, Polyarteritis | 2 | 1 2.0| |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + M + + + | 46 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + | 46 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Intestine Small | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Ileum, Hyperplasia, Lymphoid | 2 1 | 3 1.7| Jejunum, Hyperplasia, Lymphoid | 1 | 2 2.0| |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Clear Cell Focus | X | 1 | Eosinophilic Focus, Single | X | 1 | Hematopoietic Cell Proliferation | | 3 2.3| Infarct | 4 | 1 4.0| Infiltration Cellular, Lymphocytic | | 2 2.0| Inflammation, Chronic Active, Focal | 1 1 1 1 1 1 2 1 1 | 16 1.1| Tension Lipidosis | 1 1 2 1 1 1 | 9 1.3| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 38 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 204 PPM | 2| 2| 2| 2| 2| 2| 2| 5| 5| 5| 5| 5| 6| 6| 9| 9| 9| 9| 9| 9| 9| 9| 9| | L | | 2| 3| 4| 5| 6| 7| 8| 5| 6| 7| 8| 9| 0| 1| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | Bile Duct, Cyst | | 1 2.0| |__________________________________________________________________________|____________| Mesentery | + | 1 | Fat, Necrosis, Focal | 1 | 1 1.0| |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Cytoplasmic Alteration | | 1 1.0| Infiltration Cellular, Lymphocytic | 1 1 | 4 1.0| Polyarteritis | | 1 3.0| Acinus, Atrophy | 1 | 1 1.0| |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Acinus, Parotid Gland, Necrosis, | | | Focal | 1 | 3 1.3| Parotid Gland, Infiltration Cellular,| | | Lymphocytic | 2 2 1 | 8 1.5| Parotid Gland, Vacuolization | | | Cytoplasmic | | 1 2.0| Sublingual Gland, Infiltration | | | Cellular, Lymphocytic | 1 | 2 1.0| Submandibular Gland, Infiltration | | | Cellular, Lymphocytic | 2 2 2 2 3 2 2 1 2 2 2 1 1 2 2 2 | 35 1.6| |__________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Forestomach, Foreign Body | 2 | 1 2.0| Forestomach, Inflammation, Chronic | | | Active | 2 | 2 2.0| Forestomach, Epithelium, Hyperplasia,| | | Focal | 2 2 2 3 | 8 2.3| Glandular, Infiltration Cellular, | | | Lymphocytic | | 1 1.0| Glandular, Polyarteritis | | 1 2.0| |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + | 47 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 39 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 204 PPM | 2| 2| 2| 2| 2| 2| 2| 5| 5| 5| 5| 5| 6| 6| 9| 9| 9| 9| 9| 9| 9| 9| 9| | L | | 2| 3| 4| 5| 6| 7| 8| 5| 6| 7| 8| 9| 0| 1| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Tongue | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Foreign Body | | 1 1.0| Artery, Inflammation, Chronic | 1 | 2 2.0| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + | 46 | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + | 47 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Bilateral, Cortex, Hypertrophy, Focal| 2 | 1 2.0| Cortex, Accessory Adrenal Cortical | | | Nodule | X | 1 | Cortex, Hematopoietic Cell | | | Proliferation | | 1 1.0| Cortex, Hyperplasia, Focal | 2 | 2 2.0| Cortex, Hypertrophy, Focal | | 1 3.0| Cortex, Polyarteritis | | 1 3.0| Cortex, Subcapsular, Hyperplasia | 2 2 2 1 2 2 2 1 2 1 2 2 2 2 2 1 2 2 1 2 2 2 | 46 1.8| |__________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Parathyroid Gland | + + + + M + + + + + + M + M + + + + + + + + + | 41 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 40 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 204 PPM | 2| 2| 2| 2| 2| 2| 2| 5| 5| 5| 5| 5| 6| 6| 9| 9| 9| 9| 9| 9| 9| 9| 9| | L | | 2| 3| 4| 5| 6| 7| 8| 5| 6| 7| 8| 9| 0| 1| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| ENDOCRINE SYSTEM - cont | | | Pituitary Gland | + + + + + + + + + + + + + + + + + + + + M + + | 44 | Pars Distalis, Hyperplasia, Focal | 1 1 2 | 3 1.3| |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Polyarteritis | | 1 3.0| Ultimobranchial Cyst | X X | 2 | Follicular Cell, Cyst | 1 1 1 1 | 6 1.0| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | + + + + + + + + + + + + + + + + + M + + + M + | 44 | Inflammation, Subacute | | 1 2.0| Duct, Cyst | 2 2 2 2 2 2 1 2 2 2 2 2 1 2 2 1 2 2 2 2 2 | 41 1.9| |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Angiectasis | 2 2 | 2 2.0| Atrophy | 2 2 2 2 2 2 2 2 2 1 2 2 2 2 2 2 2 2 2 2 1 2 2 | 44 1.9| Cyst | X X X X X X X X X | 17 | Hemorrhage | 1 1 | 2 1.0| Mineralization | | 1 1.0| Bilateral, Cyst | X | 2 | |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Angiectasis | 1 2 | 3 1.3| Hyperplasia, Cystic | 3 2 3 3 2 1 2 4 4 2 3 2 2 4 2 4 2 3 3 1 2 4 3 | 47 2.4| Polyarteritis | | 1 2.0| Thrombosis | 3 2 2 | 3 2.3| Stroma, Fibrosis | | 1 3.0| Stroma, Hyperplasia | 3 | 1 3.0| |__________________________________________________________________________|____________| Vagina | + + + + + + + + + + + + + + + + + + + + + + + | 47 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 41 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 204 PPM | 2| 2| 2| 2| 2| 2| 2| 5| 5| 5| 5| 5| 6| 6| 9| 9| 9| 9| 9| 9| 9| 9| 9| | L | | 2| 3| 4| 5| 6| 7| 8| 5| 6| 7| 8| 9| 0| 1| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Fibrosis | | 1 3.0| Myeloid Cell, Hyperplasia | | 4 3.0| |__________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Mandibular, Hyperplasia, Lymphoid | 3 2 3 2 2 | 8 2.6| Mesenteric, Hematopoietic Cell | | | Proliferation | 2 | 1 2.0| Mesenteric, Hemorrhage | 2 | 1 2.0| Mesenteric, Hyperplasia, Lymphoid | 2 | 3 2.7| Mesenteric, Infiltration Cellular, | | | Plasma Cell | 3 3 | 2 3.0| |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + M + + + + + + | 45 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Hematopoietic Cell Proliferation | 2 1 1 1 2 2 2 | 17 2.1| Hyperplasia, Lymphoid | 1 1 2 3 3 | 8 2.1| |__________________________________________________________________________|____________| Thymus | + + + + + + + + + + + + + + + + + + + + + + M | 45 | Atrophy | | 1 2.0| Cyst | X | 2 | Ectopic Parathyroid Gland | X X X X | 4 | Hyperplasia, Lymphoid | 2 1 2 3 | 5 2.0| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + | 45 | _____________________________________________________________________________________________________________________| | * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 42 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 204 PPM | 2| 2| 2| 2| 2| 2| 2| 5| 5| 5| 5| 5| 6| 6| 9| 9| 9| 9| 9| 9| 9| 9| 9| | L | | 2| 3| 4| 5| 6| 7| 8| 5| 6| 7| 8| 9| 0| 1| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Femur, Fibrous Osteodystrophy | 3 1 1 | 5 1.6| Sternum, Degeneration, Cystic | 1 | 1 1.0| Sternum, Fibrous Osteodystrophy | 1 2 1 1 1 1 1 2 1 2 2 1 | 19 1.4| |__________________________________________________________________________|____________| Bone, Femur | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Skeletal Muscle | + + + + + + + + + + + + + + + + + + + + + + + | 47 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Cerebrum, Mineralization | 1 1 1 1 1 1 1 1 1 1 | 21 1.0| Cerebrum, Polyarteritis | 1 | 1 1.0| |__________________________________________________________________________|____________| Brain, Brain Stem | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Brain, Cerebellum | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Brain, Cerebrum | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Peripheral Nerve | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Spinal Cord | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Thoracic, Axon, Degeneration | 1 1 1 1 1 1 1 | 9 1.0| |__________________________________________________________________________|____________| Spinal Cord, Thoracic | + + + + + + + + + + + + + + + + + + + + + + + | 47 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Infiltration Cellular, Lymphocytic | 1 1 | 9 1.8| Alveolar Epithelium, Hyperplasia | 1 | 1 1.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 43 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 204 PPM | 2| 2| 2| 2| 2| 2| 2| 5| 5| 5| 5| 5| 6| 6| 9| 9| 9| 9| 9| 9| 9| 9| 9| | L | | 2| 3| 4| 5| 6| 7| 8| 5| 6| 7| 8| 9| 0| 1| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| RESPIRATORY SYSTEM - cont | | | |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + | 47 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Bilateral, Lens, Cataract | | 3 2.3| Lens, Cataract | 1 1 | 4 2.0| |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Hyperplasia | | 1 2.0| Infiltration Cellular, Lymphocytic | 1 2 1 | 8 1.1| |__________________________________________________________________________|____________| Zymbal's Gland | + + + + + + + + + + + + + + + + + + + + + + + | 46 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Infarct | | 1 1.0| Infiltration Cellular, Lymphocytic | 1 1 1 1 1 2 | 15 1.1| Nephropathy | 1 1 1 | 3 1.0| Polyarteritis | | 1 3.0| Renal Tubule, Accumulation, Hyaline | | | Droplet | | 1 2.0| Renal Tubule, Necrosis | | 1 2.0| Renal Tubule, Pigmentation | | 1 2.0| |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Inclusion Body Intracytoplasmic | 2 2 2 2 2 2 2 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 | 44 1.9| Infiltration Cellular, Lymphocytic | 1 1 2 1 1 1 1 1 1 1 1 2 1 1 3 1 | 24 1.2| Polyarteritis | | 1 1.0| Submucosa, Edema | | 1 3.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 44 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 4| 5| 5| 5| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 4| 6| 5| 8| 4| 1| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 4| 0| 3| 4| 7| 8| 4| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 408 PPM | 1| 1| 2| 2| 2| 4| 4| 4| 6| 7| 7| 7| 7| 9| 0| 0| 0| 0| 2| 3| 3| 3| 3| 3| 3| | 2| 8| 3| 5| 7| 2| 3| 4| 9| 0| 1| 2| 3| 9| 0| 1| 2| 3| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Gallbladder | + A + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Acute | 2 | Mucosa, Hyperplasia, Focal | 1 1 | |__________________________________________________________________________| Intestine Large | A + + + + + + + + + + + + + + + + + + + + + + +| Colon, Proliferation Connective | | Tissue | 2 | |__________________________________________________________________________| Intestine Large, Cecum | A + M + + + A + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Colon | A A A + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | A + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small | A + + + + + + + + + + + + + + + + + + + + + + +| Ileum, Hyperplasia, Lymphoid | | Jejunum, Hyperplasia, Lymphoid | 2 | |__________________________________________________________________________| Intestine Small, Duodenum | A + M + + + A + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Ileum | A A + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Jejunum | A + A + + + A + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | X | Basophilic Focus, Two | X | Eosinophilic Focus, Single | | Hematopoietic Cell Proliferation | 1 1 4 | Infiltration Cellular, Lymphocytic | 2 1 1 1 | Inflammation, Chronic Active, Focal | 1 1 1 1 2 1 | Leukocytosis | 2 | Tension Lipidosis | 1 1 1 | Bile Duct, Cyst | | Serosa, Fibrosis | | __________________________________________|__________________________________________________________________________| Page 45 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 4| 5| 5| 5| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 4| 6| 5| 8| 4| 1| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 4| 0| 3| 4| 7| 8| 4| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 408 PPM | 1| 1| 2| 2| 2| 4| 4| 4| 6| 7| 7| 7| 7| 9| 0| 0| 0| 0| 2| 3| 3| 3| 3| 3| 3| | 2| 8| 3| 5| 7| 2| 3| 4| 9| 0| 1| 2| 3| 9| 0| 1| 2| 3| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | |__________________________________________________________________________| Mesentery | + | Fat, Necrosis, Focal | 2 | |__________________________________________________________________________| Pancreas | A A + + + + + + + + + + + + + + + + + + + + + +| Cytoplasmic Alteration | | Infiltration Cellular, Lymphocytic | 1 2 | Acinus, Atrophy | 2 | |__________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + +| Acinus, Parotid Gland, Atrophy | | Acinus, Parotid Gland, Necrosis, | | Focal | 1 1 | Acinus, Submandibular Gland, Atrophy | | Parotid Gland, Infiltration Cellular,| | Lymphocytic | 1 1 1 2 | Sublingual Gland, Infiltration | | Cellular, Lymphocytic | | Submandibular Gland, Infiltration | | Cellular, Lymphocytic | 1 2 3 1 2 1 1 2 1 1 1 2 2 2 1 1 | |__________________________________________________________________________| Stomach | A + + + + + + + + + + + + + + + + + + + + + + +| Forestomach, Epithelium, Hyperplasia,| | Focal | 2 2 2 1 | |__________________________________________________________________________| Stomach, Forestomach | A + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Stomach, Glandular | A + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Tongue | + + + + + + + + + + + + + + + + + + + + + + + +| Foreign Body | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Blood Vessel | M + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Acute, Focal | 1 | __________________________________________|__________________________________________________________________________| Page 46 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 4| 5| 5| 5| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 4| 6| 5| 8| 4| 1| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 4| 0| 3| 4| 7| 8| 4| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 408 PPM | 1| 1| 2| 2| 2| 4| 4| 4| 6| 7| 7| 7| 7| 9| 0| 0| 0| 0| 2| 3| 3| 3| 3| 3| 3| | 2| 8| 3| 5| 7| 2| 3| 4| 9| 0| 1| 2| 3| 9| 0| 1| 2| 3| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | Mineralization | 1 | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + +| Cortex, Accessory Adrenal Cortical | | Nodule | X | Cortex, Hematopoietic Cell | | Proliferation | 3 | Cortex, Subcapsular, Hyperplasia | 2 2 1 2 2 1 2 2 2 2 2 2 2 1 2 2 2 1 1 3 1 2 2 2| |__________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Islets, Pancreatic | A A + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Parathyroid Gland | + + + + + + M + + + M + + + + + + + + + M + + +| |__________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + +| Pars Distalis, Hyperplasia, Focal | 2 | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + +| Ectopic Thymus | X | Follicular Cell, Cyst | 2 1 2 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Clitoral Gland | M + + + + + + + + + + + + + + + + + + + + M + +| Duct, Cyst | 2 1 2 2 2 2 2 2 2 2 2 2 1 2 2 2 1 2 2 1 2 2| |__________________________________________________________________________| Ovary | M I M + + + + + + + + + + + + + + + + + + + + +| Atrophy | 2 2 2 3 2 2 2 2 2 2 2 2 2 2 3 2 2 3 2| Cyst | X X X X | Hemorrhage | | __________________________________________|__________________________________________________________________________| Page 47 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 4| 5| 5| 5| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 4| 6| 5| 8| 4| 1| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 4| 0| 3| 4| 7| 8| 4| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 408 PPM | 1| 1| 2| 2| 2| 4| 4| 4| 6| 7| 7| 7| 7| 9| 0| 0| 0| 0| 2| 3| 3| 3| 3| 3| 3| | 2| 8| 3| 5| 7| 2| 3| 4| 9| 0| 1| 2| 3| 9| 0| 1| 2| 3| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| GENITAL SYSTEM - cont | | Bilateral, Cyst | X X | |__________________________________________________________________________| Uterus | A + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 1 | Hyperplasia, Cystic | 2 4 2 1 1 2 3 2 3 2 2 3 2 1 4 2 3 1 3 2 4 2| Inflammation, Acute | 3 | |__________________________________________________________________________| Vagina | M + + + + + + + + + + + + + + + + + + + + + + +| Epithelium, Hyperplasia | 2 2 | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + A + + + + + + + + + + + + + + + + + + + + + +| Myeloid Cell, Hyperplasia | 2 2 3 2 2 | |__________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + +| Lumbar, Hyperplasia, Plasma Cell | 3 | Mandibular, Hyperplasia, Lymphoid | | Mesenteric, Hemorrhage | | Mesenteric, Hyperplasia, Lymphoid | 3 3 2 | Mesenteric, Infiltration Cellular, | | Plasma Cell | 4 | Thoracic, Hyperplasia, Plasma Cell | 3 | |__________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Lymph Node, Mesenteric | A A + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Spleen | A A + + + + + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 3 3 4 3 1 3 2 1 1 2 3 | Hyperplasia, Lymphoid | 2 2 3 | Infiltration Cellular, Plasma Cell | 4 | |__________________________________________________________________________| Thymus | + M + + M M + + + + + + + + + + + + + + + + + +| Cyst | X | Ectopic Parathyroid Gland | X X X | Hyperplasia, Lymphoid | 2 2 2 | __________________________________________|__________________________________________________________________________| Page 48 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 4| 5| 5| 5| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 4| 6| 5| 8| 4| 1| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 4| 0| 3| 4| 7| 8| 4| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 408 PPM | 1| 1| 2| 2| 2| 4| 4| 4| 6| 7| 7| 7| 7| 9| 0| 0| 0| 0| 2| 3| 3| 3| 3| 3| 3| | 2| 8| 3| 5| 7| 2| 3| 4| 9| 0| 1| 2| 3| 9| 0| 1| 2| 3| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | A + M + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Lymphocytic | | |__________________________________________________________________________| Skin | A + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + +| Femur, Fibrous Osteodystrophy | | Sternum, Degeneration, Cystic | 1 1 1 | Sternum, Fibrous Osteodystrophy | 2 1 1 1 1 1 1 2| |__________________________________________________________________________| Bone, Femur | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skeletal Muscle | + + + + + M + + + + + + + + + + + + + + + + + +| Mineralization | 2 | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + +| Cerebellum, Infiltration Cellular, | | Lymphocytic | 2 | Cerebrum, Mineralization | 1 1 1 1 1 1 1 1 1 1| |__________________________________________________________________________| Brain, Brain Stem | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Brain, Cerebellum | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Brain, Cerebrum | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Peripheral Nerve | + + + + + M + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Spinal Cord | + + + + + + + + + + + + + + + + + + + + + + + +| Thoracic, Infiltration Cellular, | | Lymphocytic | 1 | Thoracic, Axon, Degeneration | 1 1 1 1 1 1| |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 49 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 4| 5| 5| 5| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 4| 6| 5| 8| 4| 1| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 4| 0| 3| 4| 7| 8| 4| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 408 PPM | 1| 1| 2| 2| 2| 4| 4| 4| 6| 7| 7| 7| 7| 9| 0| 0| 0| 0| 2| 3| 3| 3| 3| 3| 3| | 2| 8| 3| 5| 7| 2| 3| 4| 9| 0| 1| 2| 3| 9| 0| 1| 2| 3| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| NERVOUS SYSTEM - cont | | Spinal Cord, Thoracic | + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + +| Crystals | 3 | Infarct | | Infiltration Cellular, Histiocytic | 1 1 | Infiltration Cellular, Lymphocytic | 2 3 1 2 1 1 1 2 | Inflammation, Chronic, Focal | 1 | Inflammation, Granulomatous | 2 | Leukocytosis | 4 | Alveolar Epithelium, Hyperplasia | 1 | Artery, Mineralization | | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | + + + + + + + + + + + + + + + + + + + + + + + +| Phthisis Bulbi | | Bilateral, Lens, Cataract | 3 4 3 3 2 1 1 | Conjunctiva, Polyarteritis | | Lens, Cataract | 1 | |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 1 2 2 | Infiltration Cellular, Lymphocytic | 1 1 1 | |__________________________________________________________________________| Zymbal's Gland | + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + +| Glomerulosclerosis | 1 | __________________________________________|__________________________________________________________________________| Page 50 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 _____________________________________________________________________________________________________________________ | 4| 5| 5| 5| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 4| 6| 5| 8| 4| 1| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 4| 0| 3| 4| 7| 8| 4| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 408 PPM | 1| 1| 2| 2| 2| 4| 4| 4| 6| 7| 7| 7| 7| 9| 0| 0| 0| 0| 2| 3| 3| 3| 3| 3| 3| | 2| 8| 3| 5| 7| 2| 3| 4| 9| 0| 1| 2| 3| 9| 0| 1| 2| 3| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| URINARY SYSTEM - cont | | Infarct | | Infiltration Cellular, Lymphocytic | 1 1 1 1 2 1 | Nephropathy | 1 | Glomerulus, Casts Protein | 3 | Renal Tubule, Accumulation, Hyaline | | Droplet | 3 2 | |__________________________________________________________________________| Urinary Bladder | A A + + + + A + + + + + + + + + + + + + + + + +| Inclusion Body Intracytoplasmic | 2 2 2 3 3 3 2 3 3 3 3 3 3 3 3 3 3 3 3 3 3| Infiltration Cellular, Lymphocytic | 1 1 1 2 1 1 3 1 1 1 1| __________________________________________|__________________________________________________________________________ Page 51 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 8| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 408 PPM | 3| 3| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| | L | | 6| 7| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + | 46 | Inflammation, Acute | | 1 2.0| Mucosa, Hyperplasia, Focal | | 2 1.0| |__________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + + | 46 | Colon, Proliferation Connective | | | Tissue | | 1 2.0| |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + | 44 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + | 44 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + | 46 | |__________________________________________________________________________|____________| Intestine Small | + + + + + + + + + + + + + + + + + + + + + + + | 46 | Ileum, Hyperplasia, Lymphoid | 4 | 1 4.0| Jejunum, Hyperplasia, Lymphoid | | 1 2.0| |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + | 44 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + | 45 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + | 44 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Basophilic Focus | X | 2 | Basophilic Focus, Two | | 1 | Eosinophilic Focus, Single | X | 1 | Hematopoietic Cell Proliferation | | 3 2.0| Infiltration Cellular, Lymphocytic | 1 1 2 1 | 8 1.3| Inflammation, Chronic Active, Focal | 1 2 1 1 2 | 11 1.3| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 52 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 8| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 408 PPM | 3| 3| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| | L | | 6| 7| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | Leukocytosis | | 1 2.0| Tension Lipidosis | 1 1 2 1 2 1 | 9 1.2| Bile Duct, Cyst | 2 | 1 2.0| Serosa, Fibrosis | 2 | 1 2.0| |__________________________________________________________________________|____________| Mesentery | + + | 3 | Fat, Necrosis, Focal | 2 2 | 3 2.0| |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + | 45 | Cytoplasmic Alteration | 2 1 | 2 1.5| Infiltration Cellular, Lymphocytic | 1 1 1 1 | 6 1.2| Acinus, Atrophy | 4 | 2 3.0| |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Acinus, Parotid Gland, Atrophy | 3 | 1 3.0| Acinus, Parotid Gland, Necrosis, | | | Focal | | 2 1.0| Acinus, Submandibular Gland, Atrophy | 3 | 1 3.0| Parotid Gland, Infiltration Cellular,| | | Lymphocytic | 2 1 1 1 2 1 2 | 11 1.4| Sublingual Gland, Infiltration | | | Cellular, Lymphocytic | 1 | 1 1.0| Submandibular Gland, Infiltration | | | Cellular, Lymphocytic | 1 2 1 2 2 1 1 1 3 1 1 1 1 2 1 | 31 1.5| |__________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + | 46 | Forestomach, Epithelium, Hyperplasia,| | | Focal | 1 1 | 6 1.5| |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + | 46 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + | 46 | |__________________________________________________________________________|____________| Tongue | + + + + + + + + + + + + + + + + + + + + + + + | 47 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 53 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 8| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 408 PPM | 3| 3| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| | L | | 6| 7| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | Foreign Body | 1 1 1 | 3 1.0| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + + + + + + + + + + + + + + + + + + + + + + + | 46 | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Inflammation, Acute, Focal | | 1 1.0| Mineralization | | 1 1.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Cortex, Accessory Adrenal Cortical | | | Nodule | | 1 | Cortex, Hematopoietic Cell | | | Proliferation | | 1 3.0| Cortex, Subcapsular, Hyperplasia | 2 1 2 2 2 2 2 2 2 1 2 2 2 3 1 2 2 2 2 2 2 2 2 | 47 1.9| |__________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + | 45 | |__________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + | 44 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + M + + + + + + + + + + + + | 46 | Pars Distalis, Hyperplasia, Focal | 3 | 2 2.5| |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Ectopic Thymus | | 1 | Follicular Cell, Cyst | 1 1 1 1 1 2 | 9 1.3| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 54 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 8| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 408 PPM | 3| 3| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| | L | | 6| 7| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + | 45 | Duct, Cyst | 2 1 2 2 2 2 2 2 1 1 2 2 1 2 1 2 2 2 2 2 2 2 2 | 45 1.8| |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + | 44 | Atrophy | 2 2 2 3 2 2 2 3 2 2 1 2 2 2 1 1 2 2 2 2 2 2 | 41 2.0| Cyst | X X X X X X | 10 | Hemorrhage | 1 1 | 2 1.0| Bilateral, Cyst | X | 3 | |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + | 46 | Angiectasis | | 1 1.0| Hyperplasia, Cystic | 2 2 3 4 3 3 3 3 3 2 3 2 2 3 4 2 2 2 3 3 3 | 43 2.5| Inflammation, Acute | | 1 3.0| |__________________________________________________________________________|____________| Vagina | + + + + + + + + + + + + + + + + + + + + + + + | 46 | Epithelium, Hyperplasia | 1 | 3 1.7| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + | 46 | Myeloid Cell, Hyperplasia | 2 | 6 2.2| |__________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Lumbar, Hyperplasia, Plasma Cell | | 1 3.0| Mandibular, Hyperplasia, Lymphoid | 2 2 | 2 2.0| Mesenteric, Hemorrhage | 1 | 1 1.0| Mesenteric, Hyperplasia, Lymphoid | 3 3 2 | 6 2.7| Mesenteric, Infiltration Cellular, | | | Plasma Cell | | 1 4.0| Thoracic, Hyperplasia, Plasma Cell | | 1 3.0| |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 55 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 8| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 408 PPM | 3| 3| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| | L | | 6| 7| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + | 45 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + | 45 | Hematopoietic Cell Proliferation | 2 3 3 1 1 2 | 17 2.2| Hyperplasia, Lymphoid | 1 2 3 3 2 1 | 9 2.1| Infiltration Cellular, Plasma Cell | | 1 4.0| |__________________________________________________________________________|____________| Thymus | + + + + + + + + + + M + + + + + + + + + + + + | 43 | Cyst | X | 2 | Ectopic Parathyroid Gland | X X X X | 7 | Hyperplasia, Lymphoid | 1 3 1 | 6 1.8| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + | 45 | Infiltration Cellular, Lymphocytic | 2 | 1 2.0| |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + | 45 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Femur, Fibrous Osteodystrophy | 1 1 1 | 3 1.0| Sternum, Degeneration, Cystic | 1 1 | 5 1.0| Sternum, Fibrous Osteodystrophy | 1 1 2 1 1 2 1 2 1 1 1 1 1 | 21 1.2| |__________________________________________________________________________|____________| Bone, Femur | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Skeletal Muscle | + + + + + + + + + + + + + + + + + + + + + + + | 46 | Mineralization | 2 | 2 2.0| _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + | 47 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 56 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 8| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 408 PPM | 3| 3| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| | L | | 6| 7| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| NERVOUS SYSTEM - cont | | | Cerebellum, Infiltration Cellular, | | | Lymphocytic | | 1 2.0| Cerebrum, Mineralization | 1 1 1 1 1 1 1 1 1 1 1 | 21 1.0| |__________________________________________________________________________|____________| Brain, Brain Stem | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Brain, Cerebellum | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Brain, Cerebrum | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| Peripheral Nerve | + + + + + + + + + + + + + + + + + + + + + + + | 46 | |__________________________________________________________________________|____________| Spinal Cord | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Thoracic, Infiltration Cellular, | | | Lymphocytic | | 1 1.0| Thoracic, Axon, Degeneration | 2 1 1 1 1 1 3 1 | 14 1.2| |__________________________________________________________________________|____________| Spinal Cord, Thoracic | + + + + + + + + + + + + + + + + + + + + + + + | 47 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Crystals | | 1 3.0| Infarct | 2 | 1 2.0| Infiltration Cellular, Histiocytic | | 2 1.0| Infiltration Cellular, Lymphocytic | 1 1 2 1 | 12 1.5| Inflammation, Chronic, Focal | | 1 1.0| Inflammation, Granulomatous | | 1 2.0| Leukocytosis | | 1 4.0| Alveolar Epithelium, Hyperplasia | | 1 1.0| Artery, Mineralization | 1 | 1 1.0| |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + | 47 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 57 NTP Experiment-Test: 93029-04 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC LEUKOMALACHITE GREEN Date: 06/19/03 Route: DOSED FEED Time: 07:07:49 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | DAY ON TEST | 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | | | 4| 8| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | C57BL/6N XC3H/HEN MTN-NCTR FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 408 PPM | 3| 3| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| | L | | 6| 7| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| RESPIRATORY SYSTEM - cont | | | Trachea | + + + + + + + + + + + + + + + + + + + + + + + | 47 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Phthisis Bulbi | X | 1 | Bilateral, Lens, Cataract | | 7 2.4| Conjunctiva, Polyarteritis | 2 | 1 2.0| Lens, Cataract | 2 2 2 | 4 1.8| |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Hyperplasia | | 3 1.7| Infiltration Cellular, Lymphocytic | 1 1 | 5 1.0| |__________________________________________________________________________|____________| Zymbal's Gland | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Atrophy | 2 | 1 2.0| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + | 47 | Glomerulosclerosis | | 1 1.0| Infarct | 1 | 1 1.0| Infiltration Cellular, Lymphocytic | 1 2 1 2 1 | 11 1.3| Nephropathy | | 1 1.0| Glomerulus, Casts Protein | | 1 3.0| Renal Tubule, Accumulation, Hyaline | | | Droplet | 2 | 3 2.3| |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + | 44 | Inclusion Body Intracytoplasmic | 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 | 44 2.9| Infiltration Cellular, Lymphocytic | 1 1 1 2 2 1 2 1 1 2 1 1 1 1 1 | 26 1.3| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 58 ------------------------------------------------------------ ---------- END OF REPORT ---------- ------------------------------------------------------------