TDMS Study 99033-01 Pathology Tables
NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 FINAL #3 TG.AC MICE Facility: BIORELIANCE Chemical CAS #: 22839-47-0 Lock Date: 07/20/01 Cage Range: All Reasons For Removal: All Removal Date Range: All Treatment Groups: Include 001 VEHICLE CONTROL Include 002 3125 PPM Include 003 6250 PPM Include 004 12500 PPM Include 005 25000 PPM Include 006 50000 PPM Include 008 VEHICLE CONTROL Include 009 3125 PPM Include 010 6250 PPM Include 011 12500 PPM Include 012 25000 PPM Include 013 50000 PPM Note: Animals arranged according to days on test Page 1 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 0| 0| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 9| 0| 2| 0| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | VEHICLE | 1| 1| 0| 1| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 2| | L | CONTROL | 7| 1| 6| 4| 7| 8| 9| 0| 2| 3| 5| 6| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Gallbladder | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 | Squamous Cell Papilloma | X X | 2 | Squamous Cell Papilloma, Multiple | X X | 2 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Tongue | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Tooth | + + + + | 4 | Odontogenic Tumor | X X X X | 4 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 2 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 0| 0| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 9| 0| 2| 0| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | VEHICLE | 1| 1| 0| 1| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 2| | L | CONTROL | 7| 1| 6| 4| 7| 8| 9| 0| 2| 3| 5| 6| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Parathyroid Gland | M M M M + M + M + + M M M M M | 4 | |__________________________________________________________________________|____________| Pituitary Gland | + I + + + + M + + + + + + + + | 13 | |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | |__________________________________________________________________________|____________| Tissue NOS | + + | 2 | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | M + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Vagina | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 3 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 0| 0| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 9| 0| 2| 0| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | VEHICLE | 1| 1| 0| 1| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 2| | L | CONTROL | 7| 1| 6| 4| 7| 8| 9| 0| 2| 3| 5| 6| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Lymph Node | + | 1 | |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | + M + + + + + I + + + M + + + | 12 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Thymus | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + | 15 | Vulva, Squamous Cell Papilloma | X | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Skeletal Muscle | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 4 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 0| 0| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 9| 0| 2| 0| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | VEHICLE | 1| 1| 0| 1| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 2| | L | CONTROL | 7| 1| 6| 4| 7| 8| 9| 0| 2| 3| 5| 6| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| RESPIRATORY SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Larynx | M M M M M M M M I M M M M M M | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + | 15 | Alveolar/Bronchiolar Adenoma | X | 1 | |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Zymbal's Gland | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + I + + + + + + + + | 14 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | Leukemia Erythrocytic | X | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 5 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 8| 9| 9| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 1| 2| 8| 6| 1| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 3125 PPM | 3| 2| 3| 3| 3| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| | L | | 2| 1| 4| 3| 0| 2| 3| 4| 5| 6| 7| 8| 9| 1| 5| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Forestomach | A + + A + + + + + + + + + + + | 13 | Squamous Cell Papilloma | X X X X X X | 6 | Squamous Cell Papilloma, Multiple | X X X | 3 | |__________________________________________________________________________|____________| Stomach, Glandular | A A + A + + + + + + + + + + + | 12 | |__________________________________________________________________________|____________| Tooth | + + + + | 4 | Odontoma | X X X X | 4 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | A A + A + + + + + + + + + + + | 12 | |__________________________________________________________________________|____________| Adrenal Medulla | A A + A + + + + + + + + + + + | 12 | |__________________________________________________________________________|____________| Pituitary Gland | + + I A I M + + + + + + + + + | 11 | |__________________________________________________________________________|____________| Thyroid Gland | A + + A + + + + + + + + + + + | 13 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 6 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 8| 9| 9| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 1| 2| 8| 6| 1| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 3125 PPM | 3| 2| 3| 3| 3| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| | L | | 2| 1| 4| 3| 0| 2| 3| 4| 5| 6| 7| 8| 9| 1| 5| | | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | | | | Uterus | A + + A + + + + + + + + + + + | 13 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Lymph Node, Mandibular | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | A + + A + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | A M + A + + + + + + + M + + + | 11 | |__________________________________________________________________________|____________| Spleen | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Thymus | A + M + + + + + + + + + + + + | 13 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | A + + A + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Skin | + + + | 3 | Squamous Cell Papilloma | X X | 2 | Vulva, Squamous Cell Papilloma | X | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | A A + A + + + + + + + + + + + | 12 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | A + + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 7 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 8| 9| 9| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 1| 2| 8| 6| 1| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 3125 PPM | 3| 2| 3| 3| 3| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| | L | | 2| 1| 4| 3| 0| 2| 3| 4| 5| 6| 7| 8| 9| 1| 5| | | _____________________________________________________________________________________________________________________|____________| RESPIRATORY SYSTEM - cont | | | | | | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | A A + A + + + + + + + + + + + | 12 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | Leukemia Erythrocytic | X X X | 3 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 8 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 0| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 6| 6| 1| 4| 0| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 1| 7| 3| 8| 0| 7| 5| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 6250 PPM | 3| 4| 4| 3| 3| 4| 3| 4| 4| 4| 4| 4| 4| 4| 5| | L | | 8| 8| 1| 9| 7| 9| 6| 0| 2| 3| 4| 5| 6| 7| 0| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Liver | + + A + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + A A + + + + + + + + + + + | 13 | Squamous Cell Papilloma | X | 1 | Squamous Cell Papilloma, Multiple | X X X | 3 | |__________________________________________________________________________|____________| Stomach, Glandular | + + A A + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Tooth | + + | 2 | Odontoma | X X | 2 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + A A + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Adrenal Medulla | + + A A + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Pituitary Gland | M + A A + + + + + + + + + + + | 12 | |__________________________________________________________________________|____________| Thyroid Gland | + + A A + + + + + + M + + + + | 12 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Ovary | + + A A + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 9 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 0| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 6| 6| 1| 4| 0| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 1| 7| 3| 8| 0| 7| 5| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 6250 PPM | 3| 4| 4| 3| 3| 4| 3| 4| 4| 4| 4| 4| 4| 4| 5| | L | | 8| 8| 1| 9| 7| 9| 6| 0| 2| 3| 4| 5| 6| 7| 0| | | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | | | | Uterus | + + A A + + + + + + + + + + + | 13 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + A + M M + + + + + + + + + | 12 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | M + A A + + + + + + + + + + + | 12 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | + + A A + + + + M + + M + + + | 11 | |__________________________________________________________________________|____________| Spleen | + + A + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Thymus | + + A A M + + + + + + + + + + | 12 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + A A + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Skin | + | 1 | Squamous Cell Papilloma | X | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + A A + + + + + + + + + + + | 13 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + A + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | None | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 10 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 0| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 6| 6| 1| 4| 0| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 1| 7| 3| 8| 0| 7| 5| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 6250 PPM | 3| 4| 4| 3| 3| 4| 3| 4| 4| 4| 4| 4| 4| 4| 5| | L | | 8| 8| 1| 9| 7| 9| 6| 0| 2| 3| 4| 5| 6| 7| 0| | | _____________________________________________________________________________________________________________________|____________| URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + A A + + + + + + + + + + + | 13 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | Leukemia Erythrocytic | X | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 11 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 9| 3| 6| 6| 8| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 7| 4| 5| 5| 4| 1| 5| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 12500 | 5| 5| 5| 6| 6| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| | L | PPM | 6| 3| 4| 0| 4| 9| 1| 2| 5| 7| 8| 1| 2| 3| 5| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 15 | Sarcoma Stromal, Metastatic, Uterus | X | 1 | |__________________________________________________________________________|____________| Salivary Glands | + | 1 | Duct, Carcinoma | X | 1 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 | Squamous Cell Papilloma | X X X X X X X | 7 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + A + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Tooth | + + + + + | 5 | Odontoma | X X X X X | 5 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | A + A + + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Adrenal Medulla | A + A + + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Pituitary Gland | I M + M + + + + + + + + M + I | 10 | |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 12 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 9| 3| 6| 6| 8| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 7| 4| 5| 5| 4| 1| 5| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 12500 | 5| 5| 5| 6| 6| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| | L | PPM | 6| 3| 4| 0| 4| 9| 1| 2| 5| 7| 8| 1| 2| 3| 5| | | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Ovary | + + A + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Uterus | A + A + + A + + + + + + + + + | 12 | Sarcoma Stromal | X | 1 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Lymph Node | + | 1 | Pancreatic, Sarcoma Stromal, | | | Metastatic, Uterus | X | 1 | |__________________________________________________________________________|____________| Lymph Node, Mandibular | A + A M + + + + + + + + + + + | 12 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | A A + + + + + + + + I + + + + | 12 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | + + A + + + + + + + + M + + M | 12 | Carcinoma, Metastatic, Salivary | | | Glands | X | 1 | |__________________________________________________________________________|____________| Spleen | A A + + + A + + + + + + + + + | 12 | |__________________________________________________________________________|____________| Thymus | M A M + + + + + + + + + + + + | 12 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | A + + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 13 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 9| 3| 6| 6| 8| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 7| 4| 5| 5| 4| 1| 5| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 12500 | 5| 5| 5| 6| 6| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| | L | PPM | 6| 3| 4| 0| 4| 9| 1| 2| 5| 7| 8| 1| 2| 3| 5| | | _____________________________________________________________________________________________________________________|____________| NERVOUS SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Brain | A A + + + + + + + + + + + + + | 13 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + | 15 | Carcinoma, Metastatic, Salivary | | | Glands | X | 1 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | A + + + + + + + + + + + + + + | 14 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 14 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 2| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 3| 7| 9| 0| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 25000 | 7| 6| 7| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 8| | L | PPM | 8| 7| 3| 8| 6| 9| 0| 1| 2| 4| 5| 6| 7| 9| 0| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Liver | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Stomach, Forestomach | A + + + + + + + + + + + + + + | 14 | Squamous Cell Papilloma | X X X | 3 | Squamous Cell Papilloma, Multiple | X X X X | 4 | |__________________________________________________________________________|____________| Stomach, Glandular | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Tooth | + + + + + | 5 | Odontoma | X X X X X | 5 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Adrenal Medulla | M + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Pituitary Gland | A + + I + + I + I + + + + + + | 11 | |__________________________________________________________________________|____________| Thyroid Gland | A + + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Ovary | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 15 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 2| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 3| 7| 9| 0| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 25000 | 7| 6| 7| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 8| | L | PPM | 8| 7| 3| 8| 6| 9| 0| 1| 2| 4| 5| 6| 7| 9| 0| | | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | | | | Uterus | A + + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Lymph Node, Mandibular | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | A + + + M + + + M I + + + + + | 11 | |__________________________________________________________________________|____________| Spleen | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Thymus | A M + + + + + + + + + + + + + | 13 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Skin | + + + | 3 | Vulva, Squamous Cell Papilloma | X X | 2 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | A + + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | A + + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | None | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 16 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 2| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 3| 7| 9| 0| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 25000 | 7| 6| 7| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 8| | L | PPM | 8| 7| 3| 8| 6| 9| 0| 1| 2| 4| 5| 6| 7| 9| 0| | | _____________________________________________________________________________________________________________________|____________| URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | A + + + + + + + + + + + + + + | 14 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 17 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 0| 0| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 9| 9| 5| 8| 1| 6| 7| 7| 7| 7| 7| 7| 7| 7| | | | 9| 2| 2| 1| 4| 5| 9| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 50000 | 8| 8| 9| 9| 9| 9| 8| 8| 8| 8| 8| 8| 8| 9| 9| | L | PPM | 8| 2| 2| 1| 0| 4| 7| 1| 3| 4| 5| 6| 9| 3| 5| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + A A + M A + + + + + + + + + | 11 | |__________________________________________________________________________|____________| Gallbladder | A A M A M A + M + M M + + + + | 6 | |__________________________________________________________________________|____________| Intestine Large, Colon | A A A A + A + + + + + + + + + | 10 | |__________________________________________________________________________|____________| Intestine Large, Rectum | A A A A + A + + + + + + + + + | 10 | |__________________________________________________________________________|____________| Intestine Large, Cecum | A A A A + A + + + + + + + + + | 10 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | A A A A + A + + + + + + + + + | 10 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | A A A A + A + + + + + + + + + | 10 | |__________________________________________________________________________|____________| Intestine Small, Ileum | A A A A + A + + + + + + + + + | 10 | |__________________________________________________________________________|____________| Liver | + + A + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Pancreas | + A A + + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Salivary Glands | + + A + + A + + + + + + + + + | 13 | Duct, Carcinoma | X | 1 | |__________________________________________________________________________|____________| Stomach, Forestomach | + A A A + A + + + + + + + + + | 11 | Squamous Cell Papilloma | X X X X X | 5 | |__________________________________________________________________________|____________| Stomach, Glandular | A A A A + A + + + + + + + + + | 10 | |__________________________________________________________________________|____________| Tongue | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Tooth | + | 1 | Odontogenic Tumor | X | 1 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 18 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 0| 0| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 9| 9| 5| 8| 1| 6| 7| 7| 7| 7| 7| 7| 7| 7| | | | 9| 2| 2| 1| 4| 5| 9| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 50000 | 8| 8| 9| 9| 9| 9| 8| 8| 8| 8| 8| 8| 8| 9| 9| | L | PPM | 8| 2| 2| 1| 0| 4| 7| 1| 3| 4| 5| 6| 9| 3| 5| | | _____________________________________________________________________________________________________________________|____________| CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | A A A + + A + + M + + + + + + | 10 | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + M + + + | 14 | |__________________________________________________________________________|____________| Islets, Pancreatic | A A A A + + + + + + + + + + + | 11 | |__________________________________________________________________________|____________| Parathyroid Gland | M M M A M M I M M M M + M + + | 3 | |__________________________________________________________________________|____________| Pituitary Gland | M M M A + A + + + I + + + + + | 9 | |__________________________________________________________________________|____________| Thyroid Gland | + A A A + + + + + + M + + + + | 11 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | + + A M M A + + M + + + + + + | 10 | |__________________________________________________________________________|____________| Ovary | + + A + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Uterus | + + + A + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Vagina | A A A + + A + + + + + + + + + | 11 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 19 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 0| 0| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 9| 9| 5| 8| 1| 6| 7| 7| 7| 7| 7| 7| 7| 7| | | | 9| 2| 2| 1| 4| 5| 9| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 50000 | 8| 8| 9| 9| 9| 9| 8| 8| 8| 8| 8| 8| 8| 9| 9| | L | PPM | 8| 2| 2| 1| 0| 4| 7| 1| 3| 4| 5| 6| 9| 3| 5| | | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Bone Marrow | A A A + + + + + + + + + + + + | 12 | |__________________________________________________________________________|____________| Lymph Node, Mandibular | A + A + + A + + + + + + + + + | 12 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | A A A A + A + + + + + + + M + | 9 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | A A A M + + + + + + + + + + + | 11 | |__________________________________________________________________________|____________| Spleen | + + A + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Thymus | + A A A + A + + + + + + + + + | 11 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | A A A + + + + + M + + + + + + | 11 | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Skeletal Muscle | + + + + + A + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | A A A + + A + + + + + + + + + | 11 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Larynx | M M M M M M M M I M M M I M I | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 20 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 0| 0| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 9| 9| 5| 8| 1| 6| 7| 7| 7| 7| 7| 7| 7| 7| | | | 9| 2| 2| 1| 4| 5| 9| 5| 5| 5| 5| 5| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 50000 | 8| 8| 9| 9| 9| 9| 8| 8| 8| 8| 8| 8| 8| 9| 9| | L | PPM | 8| 2| 2| 1| 0| 4| 7| 1| 3| 4| 5| 6| 9| 3| 5| | | _____________________________________________________________________________________________________________________|____________| RESPIRATORY SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Lung | + + A + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Nose | A + A + + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Trachea | A A A A + A + + + + + + + + + | 10 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | A A A A + A + + + + + + + + + | 10 | |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Zymbal's Gland | + + M + + + + + + + M + + + + | 13 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + A + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Urinary Bladder | A A A A + A + + + + + + + + + | 10 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | Leukemia Erythrocytic | X | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 21 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 8| 9| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 6| 3| 7| 1| 1| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | VEHICLE | 0| 1| 1| 0| 1| 1| 0| 0| 0| 0| 0| 0| 0| 1| 1| | L | CONTROL | 4| 4| 5| 6| 2| 3| 1| 2| 3| 5| 7| 8| 9| 0| 1| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + A + + + + + + + + + + + M + | 13 | |__________________________________________________________________________|____________| Gallbladder | A A A + + + + + + M + + M + + | 10 | |__________________________________________________________________________|____________| Intestine Large, Colon | A A A + + + + + + + + + + + + | 12 | |__________________________________________________________________________|____________| Intestine Large, Rectum | A A A + + + + + + + + + + + + | 12 | |__________________________________________________________________________|____________| Intestine Large, Cecum | A A A + + + + + + + + + + + + | 12 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | A A A + + + + + + + + + + + + | 12 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | A A A + + + + + + + + + + + + | 12 | |__________________________________________________________________________|____________| Intestine Small, Ileum | A A A + + + + + + + + + + + + | 12 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Pancreas | + A A + + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Salivary Glands | + A A + + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Stomach, Forestomach | A A A + + + + + + + + + + + + | 12 | Squamous Cell Papilloma | X X X X | 4 | Squamous Cell Papilloma, Multiple | X X X X | 4 | |__________________________________________________________________________|____________| Stomach, Glandular | A A A + + + + + + + + + + + + | 12 | |__________________________________________________________________________|____________| Tongue | + A A + + M + + + + + + + + + | 12 | |__________________________________________________________________________|____________| Tooth | + + + + | 4 | Odontogenic Tumor | X X X | 3 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 22 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 8| 9| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 6| 3| 7| 1| 1| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | VEHICLE | 0| 1| 1| 0| 1| 1| 0| 0| 0| 0| 0| 0| 0| 1| 1| | L | CONTROL | 4| 4| 5| 6| 2| 3| 1| 2| 3| 5| 7| 8| 9| 0| 1| | | _____________________________________________________________________________________________________________________|____________| CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + A A + + + + + + + + + + M + | 12 | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + A A + + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Adrenal Medulla | + A A + + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Islets, Pancreatic | + A A + + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Parathyroid Gland | + A A + M M + M + M I M M + M | 5 | |__________________________________________________________________________|____________| Pituitary Gland | I A A + + + + + + + + + I I I | 9 | |__________________________________________________________________________|____________| Thyroid Gland | A M + + + + + + + + + + + + + | 13 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Preputial Gland | + A A + + + + + + + + M + + + | 12 | |__________________________________________________________________________|____________| Prostate | + A A + + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Seminal Vesicle | + A A + + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 23 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 8| 9| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 6| 3| 7| 1| 1| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | VEHICLE | 0| 1| 1| 0| 1| 1| 0| 0| 0| 0| 0| 0| 0| 1| 1| | L | CONTROL | 4| 4| 5| 6| 2| 3| 1| 2| 3| 5| 7| 8| 9| 0| 1| | | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + A + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Lymph Node, Mandibular | + A M + + + + + + M + + + + + | 12 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + A A + + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | + M A + I I + + + + + + + + + | 11 | |__________________________________________________________________________|____________| Spleen | + A + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Thymus | A A A + + + + + + + + + + + + | 12 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M | | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + A A + + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Skeletal Muscle | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Larynx | I A A I M I + I I I + I + M M | 3 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 24 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 8| 9| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 6| 3| 7| 1| 1| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | VEHICLE | 0| 1| 1| 0| 1| 1| 0| 0| 0| 0| 0| 0| 0| 1| 1| | L | CONTROL | 4| 4| 5| 6| 2| 3| 1| 2| 3| 5| 7| 8| 9| 0| 1| | | _____________________________________________________________________________________________________________________|____________| RESPIRATORY SYSTEM - cont | | | | | | Lung | + A + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Nose | + A + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Trachea | + A A + + + + + + + + + + + + | 13 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | A A A + + + + + + + + + + + + | 12 | |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Zymbal's Gland | + A + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Urinary Bladder | + A A + + + + + + + + + + + + | 13 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | Leukemia Erythrocytic | X | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 25 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 9| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 8| 1| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 3125 PPM | 2| 2| 2| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 3| | L | | 9| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 6| 7| 8| 0| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 | Squamous Cell Papilloma | X | 1 | Squamous Cell Papilloma, Multiple | X X X X | 4 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Tooth | + + + + | 4 | Odontogenic Tumor | X X X X | 4 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Pituitary Gland | + + I + + + M + + + + + + + + | 13 | |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 26 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 9| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 8| 1| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 3125 PPM | 2| 2| 2| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 3| | L | | 9| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 6| 7| 8| 0| | | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | | | | Penis | + | 1 | |__________________________________________________________________________|____________| Preputial Gland | + | 1 | |__________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | + + + + + + + + + + + M + M + | 13 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Thymus | M + + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M | | |__________________________________________________________________________|____________| Skin | + + | 2 | Squamous Cell Papilloma | X X | 2 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 27 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 9| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 8| 1| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 3125 PPM | 2| 2| 2| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 3| | L | | 9| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 6| 7| 8| 0| | | _____________________________________________________________________________________________________________________|____________| NERVOUS SYSTEM - cont | | | | | | Brain | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 28 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 3| 3| 5| 9| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 8| 2| 8| 0| 7| 7| 1| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 6250 PPM | 3| 3| 4| 3| 3| 4| 3| 3| 3| 3| 3| 4| 4| 4| 4| | L | | 9| 3| 3| 7| 4| 2| 1| 2| 5| 6| 8| 0| 1| 4| 5| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Liver | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Salivary Glands | + + | 2 | Duct, Carcinoma | X X | 2 | |__________________________________________________________________________|____________| Stomach, Forestomach | A A + + + + + + + + + + + + + | 13 | Squamous Cell Papilloma | X | 1 | Squamous Cell Papilloma, Multiple | X | 1 | |__________________________________________________________________________|____________| Stomach, Glandular | A A + + + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Tooth | + + | 2 | Odontogenic Tumor | X X | 2 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | A A + + + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Adrenal Medulla | A A + + + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Pituitary Gland | A M M I + + I + + + I M I I + | 6 | |__________________________________________________________________________|____________| Thyroid Gland | M A + + + + + + + + + + + + + | 13 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 29 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 3| 3| 5| 9| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 8| 2| 8| 0| 7| 7| 1| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 6250 PPM | 3| 3| 4| 3| 3| 4| 3| 3| 3| 3| 3| 4| 4| 4| 4| | L | | 9| 3| 3| 7| 4| 2| 1| 2| 5| 6| 8| 0| 1| 4| 5| | | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Coagulating Gland | + | 1 | |__________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Preputial Gland | + | 1 | |__________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Lymph Node, Mandibular | A + + + + + + + + + + M + + + | 13 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | A + + M + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | M M + + + + M M + + + + + + + | 11 | |__________________________________________________________________________|____________| Spleen | A A + + + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Thymus | M M + + + M + + + + + + + + + | 12 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M | | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 30 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 3| 3| 5| 9| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 8| 2| 8| 0| 7| 7| 1| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 6250 PPM | 3| 3| 4| 3| 3| 4| 3| 3| 3| 3| 3| 4| 4| 4| 4| | L | | 9| 3| 3| 7| 4| 2| 1| 2| 5| 6| 8| 0| 1| 4| 5| | | _____________________________________________________________________________________________________________________|____________| NERVOUS SYSTEM - cont | | | | | | Brain | A A + + + + + + + + + + + + + | 13 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | A + + + + + + + + + + + + + + | 14 | Alveolar/Bronchiolar Adenoma | X X | 2 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | A A + + + + + + + + + + + + + | 13 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | Leukemia Erythrocytic | X | 1 | Lymphoma Malignant | X | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 31 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 9| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 9| 6| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 12500 | 5| 5| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 6| | L | PPM | 8| 0| 6| 7| 8| 9| 1| 2| 3| 4| 5| 6| 7| 9| 0| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Forestomach | A + + + + + + + + + + + + + + | 14 | Squamous Cell Papilloma | X X X | 3 | Squamous Cell Papilloma, Multiple | X X | 2 | |__________________________________________________________________________|____________| Stomach, Glandular | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Tooth | + + | 2 | Odontogenic Tumor | X X | 2 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Adrenal Medulla | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Pituitary Gland | M + + + I + + I + + I + + + + | 11 | |__________________________________________________________________________|____________| Thyroid Gland | A + + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 32 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 9| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 9| 6| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 12500 | 5| 5| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 6| | L | PPM | 8| 0| 6| 7| 8| 9| 1| 2| 3| 4| 5| 6| 7| 9| 0| | | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | | | | Prostate | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Testes | A + + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Lymph Node, Mandibular | + M + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | + + + + + M + M M + I + + + M | 10 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Thymus | A M + + + + + + + + + + + + + | 13 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M | | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | A + + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + | 15 | Alveolar/Bronchiolar Adenoma | X | 1 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | None | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 33 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 9| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 9| 6| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 12500 | 5| 5| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 6| | L | PPM | 8| 0| 6| 7| 8| 9| 1| 2| 3| 4| 5| 6| 7| 9| 0| | | _____________________________________________________________________________________________________________________|____________| URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | Leukemia Erythrocytic | X X | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 34 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 9| 3| 3| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 6| 8| 9| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 25000 | 6| 7| 6| 7| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| | L | PPM | 1| 5| 8| 2| 2| 3| 4| 5| 6| 7| 9| 0| 1| 3| 4| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Liver | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Salivary Glands | + + | 2 | Duct, Carcinoma | X | 1 | |__________________________________________________________________________|____________| Stomach, Forestomach | A + + + + + + + + + + + + + + | 14 | Squamous Cell Papilloma | X X X X | 4 | Squamous Cell Papilloma, Multiple | X X | 2 | |__________________________________________________________________________|____________| Stomach, Glandular | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Tooth | + + | 2 | Odontogenic Tumor | X X | 2 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Pituitary Gland | A + + + + + M + + + + + + + + | 13 | |__________________________________________________________________________|____________| Thyroid Gland | A + + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 35 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 9| 3| 3| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 6| 8| 9| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 25000 | 6| 7| 6| 7| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| | L | PPM | 1| 5| 8| 2| 2| 3| 4| 5| 6| 7| 9| 0| 1| 3| 4| | | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Seminal Vesicle | + | 1 | |__________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Lymph Node, Mandibular | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Spleen | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Thymus | I + + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M | | |__________________________________________________________________________|____________| Skin | + | 1 | Squamous Cell Papilloma | X | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 36 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 0| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 9| 3| 3| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 6| 8| 9| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 25000 | 6| 7| 6| 7| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| | L | PPM | 1| 5| 8| 2| 2| 3| 4| 5| 6| 7| 9| 0| 1| 3| 4| | | _____________________________________________________________________________________________________________________|____________| NERVOUS SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Brain | A + + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | A + + + + + + + + + + + + + + | 14 | Alveolar/Bronchiolar Adenoma | X X | 2 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Zymbal's Gland | + | 1 | Carcinoma | X | 1 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | A + + + + + + + + + + + + + + | 14 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 37 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 4| 8| 8| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 1| 0| 0| 0| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 50000 | 8| 8| 9| 8| 8| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| | L | PPM | 9| 4| 0| 0| 3| 6| 7| 8| 9| 1| 2| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Gallbladder | A + + + + M + M + + + + + + + | 12 | |__________________________________________________________________________|____________| Intestine Large, Colon | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Intestine Large, Rectum | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Intestine Large, Cecum | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Intestine Small, Ileum | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Pancreas | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Salivary Glands | A + + + + + + + + + + + + + + | 14 | Duct, Carcinoma | X | 1 | |__________________________________________________________________________|____________| Stomach, Forestomach | A + + + + + + + + + + + + + + | 14 | Squamous Cell Papilloma | X X X X X | 5 | Squamous Cell Papilloma, Multiple | X | 1 | |__________________________________________________________________________|____________| Stomach, Glandular | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Tongue | A + + + + + + + + + + + M + + | 13 | |__________________________________________________________________________|____________| Tooth | + + + + + + | 6 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 38 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 4| 8| 8| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 1| 0| 0| 0| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 50000 | 8| 8| 9| 8| 8| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| | L | PPM | 9| 4| 0| 0| 3| 6| 7| 8| 9| 1| 2| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | | | | Odontogenic Tumor | X X X X X X | 6 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | A + + + + + + + + + + + + + I | 13 | |__________________________________________________________________________|____________| Adrenal Medulla | A + + + + + + + + + + + + + M | 13 | |__________________________________________________________________________|____________| Islets, Pancreatic | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Parathyroid Gland | A M M M + M + M M + M M M M + | 4 | |__________________________________________________________________________|____________| Pituitary Gland | A + I M + + + I + + + + I + I | 9 | |__________________________________________________________________________|____________| Thyroid Gland | A + + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Coagulating Gland | + | 1 | |__________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Penis | + | 1 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 39 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 4| 8| 8| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 1| 0| 0| 0| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 50000 | 8| 8| 9| 8| 8| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| | L | PPM | 9| 4| 0| 0| 3| 6| 7| 8| 9| 1| 2| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Preputial Gland | A + + + + + + M + + + + M + + | 12 | |__________________________________________________________________________|____________| Prostate | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Seminal Vesicle | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Lymph Node, Mandibular | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | A + M + + + + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Spleen | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Thymus | A M M + + + + + + + + + + + + | 12 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M | | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | A + + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 40 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 4| 8| 8| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 1| 0| 0| 0| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 50000 | 8| 8| 9| 8| 8| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| | L | PPM | 9| 4| 0| 0| 3| 6| 7| 8| 9| 1| 2| 5| 6| 7| 8| | | _____________________________________________________________________________________________________________________|____________| MUSCULOSKELETAL SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Skeletal Muscle | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Larynx | M M M M M + M M + I M M M M M | 2 | |__________________________________________________________________________|____________| Lung | A + + + + + + + + + + + + + + | 14 | Alveolar/Bronchiolar Adenoma | X X | 2 | |__________________________________________________________________________|____________| Nose | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Trachea | A + + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | A + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + | 15 | Adenoma | X | 1 | |__________________________________________________________________________|____________| Zymbal's Gland | A + + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Urinary Bladder | A + + + + + + + + + + + + + + | 14 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 41 NTP Experiment-Test: 99033-01 NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED) Report: PEIRPT17 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:30:26 __________________________________________________________________________________________________________________________________ | 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 4| 8| 8| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 1| 0| 0| 0| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | _____________________________________________________________________________________________________________________ T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | TGAC (FVB/N) HEMIZYGOUS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A | 50000 | 8| 8| 9| 8| 8| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| | L | PPM | 9| 4| 0| 0| 3| 6| 7| 8| 9| 1| 2| 5| 6| 7| 8| | | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS - cont | | | | | | Lymphoma Malignant | X | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 42 ------------------------------------------------------------ ---------- END OF REPORT ---------- ------------------------------------------------------------