TDMS Study 99033-02 Pathology Tables
NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 FINAL #3/P53 MICE Facility: BIORELIANCE Chemical CAS #: 22839-47-0 Lock Date: 07/20/01 Cage Range: All Reasons For Removal: All Removal Date Range: All Treatment Groups: Include All Note: Animals arranged according to CID number Page 1 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 3| 7| 7| | | | 8| 8| 8| 5| 8| 8| 5| 5| 8| 5| 5| 8| 6| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | VEHICLE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| | L | CONTROL | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Gallbladder | + + + M + + + + + + + + + + + | 14 | Lymphoma Malignant | X | 1 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 15 | Lymphoma Malignant | X | 1 | |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Tongue | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 2 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 3| 7| 7| | | | 8| 8| 8| 5| 8| 8| 5| 5| 8| 5| 5| 8| 6| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | VEHICLE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| | L | CONTROL | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| CARDIOVASCULAR SYSTEM - cont | | | | | | Blood Vessel | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + M + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Parathyroid Gland | M M M M + M M + M + + M M + + | 6 | |__________________________________________________________________________|____________| Pituitary Gland | M M + + I + + + I + + + I I + | 9 | Pars Distalis, Adenoma | X | 1 | |__________________________________________________________________________|____________| Thyroid Gland | + + + M + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | M + + + + + + + + + + + M + M | 12 | |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Vagina | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 3 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 3| 7| 7| | | | 8| 8| 8| 5| 8| 8| 5| 5| 8| 5| 5| 8| 6| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | VEHICLE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| | L | CONTROL | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | M + + + + + M + + + + + + M + | 12 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + | 15 | Lymphoma Malignant | X | 1 | |__________________________________________________________________________|____________| Thymus | M + + + + + + + + + + + + + + | 14 | Lymphoma Malignant | X | 1 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + M M + + + + + + + | 13 | Carcinoma | X | 1 | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + | 15 | Femur, Osteosarcoma | X | 1 | |__________________________________________________________________________|____________| Skeletal Muscle | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 4 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 3| 7| 7| | | | 8| 8| 8| 5| 8| 8| 5| 5| 8| 5| 5| 8| 6| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | VEHICLE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| | L | CONTROL | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Larynx | M M M + M M M + M + I I + M M | 4 | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + | 15 | Lymphoma Malignant | X | 1 | |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Zymbal's Gland | M M + M + + + + + + + + + + + | 12 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + | 15 | Lymphoma Malignant | X | 1 | |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + M + + | 14 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | Lymphoma Malignant | X | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 5 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 9| 7| 7| 7| 7| 7| | | | 8| 5| 8| 5| 8| 8| 8| 8| 8| 8| 5| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 3125 PPM | 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| | L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + | 1 | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + M + + + + + | 14 | |__________________________________________________________________________|____________| Thyroid Gland | + M + + + + + + + A + + + + + | 13 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 6 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 9| 7| 7| 7| 7| 7| | | | 8| 5| 8| 5| 8| 8| 8| 8| 8| 8| 5| 8| 8| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 3125 PPM | 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| | L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + A + + + M + | 13 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | + + + + + + + + M M + + + + + | 13 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Thymus | + + + + + + + + + M + + + + + | 14 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + A + + + + + | 14 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + A + + + + + | 14 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + A + + + + + | 14 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 7 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 1| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| | | | 8| 5| 8| 8| 5| 5| 8| 8| 5| 5| 8| 8| 5| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 6250 PPM | 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + I + + + + | 14 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + I + + + + | 14 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + I + + + + | 14 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + I + + + + | 14 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + I + + + + | 14 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + I + M + + + + | 13 | |__________________________________________________________________________|____________| Thyroid Gland | M + + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + I + + + + | 14 | |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Lymph Node, Mandibular | M + + + + + + M + + + + + + + | 13 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 8 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 1| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| | | | 8| 5| 8| 8| 5| 5| 8| 8| 5| 5| 8| 8| 5| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 6250 PPM | 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + M + + + + | 14 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | + + + + + + + + + + M + M + + | 13 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Thymus | + + + + + + + + + + I + + + + | 14 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + M + + + + | 14 | |__________________________________________________________________________|____________| Skin | + | 1 | Subcutaneous Tissue, Fibrosarcoma | X | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + | 1 | Vertebra, Osteosarcoma | X | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + I + + + + | 14 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + I + + + + | 14 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 9 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 1| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| | | | 8| 5| 8| 8| 5| 5| 8| 8| 5| 5| 8| 8| 5| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 6250 PPM | 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 10 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 5| 8| 8| 5| 5| 8| 8| 5| 5| 5| 8| 5| 8| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 12500 | 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| | L | PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 15 | Sarcoma | X | 1 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + I | 14 | |__________________________________________________________________________|____________| Thyroid Gland | + + + + + I + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 11 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 5| 8| 8| 5| 5| 8| 8| 5| 5| 5| 8| 5| 8| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 12500 | 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| | L | PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | | | | Lymph Node, Mandibular | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + M + + + + + + + + + + + + | 14 | Sarcoma | X | 1 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | + M + + + + + + + M + M + + + | 12 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Thymus | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + | 15 | Carcinoma | X | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 12 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 5| 8| 8| 5| 5| 8| 8| 5| 5| 5| 8| 5| 8| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 12500 | 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| | L | PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| URINARY SYSTEM - cont | | | | | | Kidney | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 13 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 5| 8| 8| 5| 8| 8| 5| 8| 5| 8| 8| 8| 8| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 25000 | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | L | PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Intestine Large, Cecum | + | 1 | Lymphoid Tissue, Lymphoma Malignant | X | 1 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Pituitary Gland | + I + + + M + + M + I + + + + | 11 | |__________________________________________________________________________|____________| Thyroid Gland | + I + + + + + + + M + + + + + | 13 | Follicular Cell, Adenoma | X | 1 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 14 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 5| 8| 8| 5| 8| 8| 5| 8| 5| 8| 8| 8| 8| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 25000 | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | L | PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Lymph Node | + | 1 | Pancreatic, Lymphoma Malignant | X | 1 | Renal, Lymphoma Malignant | X | 1 | |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + | 15 | Lymphoma Malignant | X | 1 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | + + + + + I + + M + + + + + + | 13 | Lymphoma Malignant | X | 1 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + | 15 | Lymphoma Malignant | X | 1 | |__________________________________________________________________________|____________| Thymus | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + M + M + M + + | 12 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | None | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 15 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 5| 8| 8| 5| 8| 8| 5| 8| 5| 8| 8| 8| 8| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 25000 | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | L | PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | Lymphoma Malignant | X | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 16 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 5| 8| 5| 8| 8| 8| 8| 5| 5| 5| 8| 8| 5| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 50000 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| | L | PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + M + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Tongue | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 17 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 5| 8| 5| 8| 8| 8| 8| 5| 5| 5| 8| 8| 5| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 50000 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| | L | PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| CARDIOVASCULAR SYSTEM - cont | | | | | | Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Parathyroid Gland | + + + + + + M + M + + + M M + | 11 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + M + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Vagina | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 18 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 5| 8| 5| 8| 8| 8| 8| 5| 5| 5| 8| 8| 5| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 50000 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| | L | PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + M + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Thymus | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + | 15 | Femur, Osteosarcoma | X | 1 | |__________________________________________________________________________|____________| Skeletal Muscle | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Larynx | I + M M + + I I I + I I I + I | 5 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 19 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 5| 8| 5| 8| 8| 8| 8| 5| 5| 5| 8| 8| 5| 8| 8| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 50000 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| | L | PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| RESPIRATORY SYSTEM - cont | | | | | | Lung | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Zymbal's Gland | + + + + + + M + + M + + M M + | 11 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 20 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 1| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7| | | | 4| 5| 4| 4| 4| 4| 4| 5| 4| 2| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | VEHICLE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | L | CONTROL | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Gallbladder | I + + + + + + + + A + + + + + | 13 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Tongue | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + + + + + + + + + + + + + M + | 14 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 21 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 1| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7| | | | 4| 5| 4| 4| 4| 4| 4| 5| 4| 2| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | VEHICLE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | L | CONTROL | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| CARDIOVASCULAR SYSTEM - cont | | | | | | Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Parathyroid Gland | + M + M M M M + M M + + M + M | 6 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + I + + I + + + + + | 13 | |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + A + + + + + | 14 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Preputial Gland | M + + + + + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Prostate | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 22 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 1| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7| | | | 4| 5| 4| 4| 4| 4| 4| 5| 4| 2| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | VEHICLE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | L | CONTROL | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | + + M + + + + + + A + + + + + | 13 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Thymus | + + + + + + + + + A + + + + + | 14 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M | | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Skeletal Muscle | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Peripheral Nerve | + | 1 | |__________________________________________________________________________|____________| Spinal Cord | + | 1 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 23 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 1| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7| | | | 4| 5| 4| 4| 4| 4| 4| 5| 4| 2| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | VEHICLE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | L | CONTROL | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Larynx | M + M M M M M M M M + M M + + | 4 | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Nose | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Trachea | + + + + + + + + + A + + + + + | 14 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Zymbal's Gland | + + + + + + + + + + M + M M + | 12 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + A + + + + + | 14 | |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + A + + + + + | 14 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 24 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 5| 4| 4| 5| 4| 5| 4| 4| 5| 5| 4| 5| 5| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 3125 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| | L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Salivary Glands | + | 1 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Pituitary Gland | + + I + + I + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 25 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 5| 4| 4| 5| 4| 5| 4| 4| 5| 5| 4| 5| 5| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 3125 PPM | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| | L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + M + + + + + + | 14 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | + + + + + + + + M + + + + + + | 14 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Thymus | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M | | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 26 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 1| 7| 7| 1| 7| 7| 7| 7| 7| 7| | | | 4| 4| 4| 5| 4| 4| 5| 4| 0| 5| 4| 4| 5| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 6250 PPM | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 15 | Lymphoma Malignant | X X | 2 | |__________________________________________________________________________|____________| Salivary Glands | + | 1 | Lymphoma Malignant | X | 1 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Pituitary Gland | I I + + + + I + I + + + + + + | 11 | |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 27 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 1| 7| 7| 1| 7| 7| 7| 7| 7| 7| | | | 4| 4| 4| 5| 4| 4| 5| 4| 0| 5| 4| 4| 5| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 6250 PPM | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Lymph Node, Mandibular | I + + + + + + + + + + + + + + | 14 | Lymphoma Malignant | X | 1 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + | 15 | Lymphoma Malignant | X | 1 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | + + M + + + + + + + + + + + + | 14 | Lymphoma Malignant | X X | 2 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + | 15 | Lymphoma Malignant | X X | 2 | |__________________________________________________________________________|____________| Thymus | + + + + + + + + + + + + + + + | 15 | Lymphoma Malignant | X X | 2 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M | | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 28 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 1| 7| 7| 1| 7| 7| 7| 7| 7| 7| | | | 4| 4| 4| 5| 4| 4| 5| 4| 0| 5| 4| 4| 5| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 6250 PPM | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| | L | | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| RESPIRATORY SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + | 15 | Lymphoma Malignant | X X | 2 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + | 1 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + | 15 | Lymphoma Malignant | X | 1 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | Lymphoma Malignant | X X | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 29 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 5| 4| 4| 4| 4| 4| 4| 5| 4| 4| 4| 5| 4| 5| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 12500 | 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| | L | PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Pituitary Gland | M + M I I I + + I I + + + + + | 8 | |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + I | 14 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 30 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 5| 4| 4| 4| 4| 4| 4| 5| 4| 4| 4| 5| 4| 5| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 12500 | 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| | L | PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Lymph Node | + | 1 | |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + I + + + M + + + + | 13 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | + + M + + + M + + + + + + + + | 13 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Thymus | M + + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M | | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 31 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 5| 4| 4| 4| 4| 4| 4| 5| 4| 4| 4| 5| 4| 5| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 12500 | 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| | L | PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| URINARY SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 32 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 4| 4| 4| 4| 9| 4| 5| 4| 4| 4| 5| 4| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 25000 | 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| | L | PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Liver | + + + + A + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Salivary Glands | + | 1 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + A + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + A + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Pituitary Gland | + M + I A + + I + + + + + I + | 10 | |__________________________________________________________________________|____________| Thyroid Gland | + + + + A + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Epididymis | + + + + A + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Prostate | + + + + A + + + + + + + + + + | 14 | Sarcoma | X | 1 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 33 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 4| 4| 4| 4| 9| 4| 5| 4| 4| 4| 5| 4| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 25000 | 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| | L | PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | | | | Testes | + + + + A + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + M + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + M + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | + + + + M + + M + + + + M + + | 12 | |__________________________________________________________________________|____________| Spleen | + + + + A + + + + + + + + + + | 14 | |__________________________________________________________________________|____________| Thymus | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M | | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + A + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + A + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 34 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 4| 4| 4| 4| 9| 4| 5| 4| 4| 4| 5| 4| 5| 5| 5| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 25000 | 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| | L | PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | | _____________________________________________________________________________________________________________________|____________| URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + A + + + + + + + + + + | 14 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 35 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 4| 4| 5| 4| 5| 1| 4| 5| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| | A | 50000 | 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| | L | PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Gallbladder | + + M + + + + + + + M + M + + | 12 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + | 15 | Polyp Adenomatous | X | 1 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Tongue | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Tooth | + | 1 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 36 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 4| 4| 5| 4| 5| 1| 4| 5| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| | A | 50000 | 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| | L | PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| CARDIOVASCULAR SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Blood Vessel | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Parathyroid Gland | + + + M + M + + + + + + + + + | 13 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + I I + + + + + + M + + | 12 | |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Preputial Gland | + + + + + + + M + M + + + + + | 13 | |__________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + | 15 | Sarcoma | X | 1 | |__________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 37 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 4| 4| 5| 4| 5| 1| 4| 5| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| | A | 50000 | 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| | L | PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | | | | |__________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Lymph Node, Mediastinal | + M + + + + + + + + + M + + + | 13 | |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Thymus | + + + + + I + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M | | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + | 15 | Subcutaneous Tissue, Sarcoma | X | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Skeletal Muscle | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 38 NTP Experiment-Test: 99033-02 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: 26-39 WEEKS TRANSGENIC MODEL EVALUATION II (ASPARTAME) Date: 03/05/03 Route: DOSED FEED Time: 11:33:02 __________________________________________________________________________________________________________________________________ | 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| | | DAY ON TEST | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | | | 4| 4| 5| 4| 5| 1| 4| 5| 4| 4| 4| 4| 4| 4| 4| | | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | P53+/-(C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| | A | 50000 | 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| | L | PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | | _____________________________________________________________________________________________________________________|____________| NERVOUS SYSTEM - cont | | | | | | Brain | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Larynx | M + M M M M M I M M I M + M M | 2 | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + | 15 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Zymbal's Gland | + M + + + + + + + + + + + + + | 14 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + | 15 | |__________________________________________________________________________|____________| Urinary Bladder | + + M + + + + + + + + + + + + | 14 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | + + + + + + + + + + + + + + + | 15 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 39 ------------------------------------------------------------ ---------- END OF REPORT ---------- ------------------------------------------------------------