TDMS Study 88004-06 Pathology Tables
NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT18 Study Type: CHRONIC WITH AVERAGE SEVERITY GRADES[b] Date: 08/14/03 Route: RESPIRATORY EXPOSURE WHOLE BODY DIVINYLBENZENE Time: 13:13:26 FINAL #1 MICE Facility: Battelle Northwest Chemical CAS #: 1321-74-0 Lock Date: 06/17/02 Cage Range: All Reasons For Removal: All Removal Date Range: All Treatment Groups: Include All a Number of animals examined microscopically at site and number of animals with lesion b Average severity grade (1-minimal;2-mild;3-moderate;4-marked) Page 1 NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT18 Study Type: CHRONIC WITH AVERAGE SEVERITY GRADES[b] Date: 08/14/03 Route: RESPIRATORY EXPOSURE WHOLE BODY DIVINYLBENZENE Time: 13:13:26 ____________________________________________________________________________________________________________________________________ B6C3F1 MICE FEMALE CONTROL 10 PPM 30 PPM 100 PPM ____________________________________________________________________________________________________________________________________ DISPOSITION SUMMARY Animals Initially In Study 50 50 50 50 Early Deaths Moribund Sacrifice 11 12 8 7 Natural Death 6 3 3 1 Accidently Killed 1 Survivors Terminal Sacrifice 33 35 38 42 Animals Examined Microscopically 50 50 50 50 ____________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM Esophagus (50) (50) (50) (50) Infiltration Cellular, Mixed Cell 1 [3.0] Gallbladder (45) (41) (36) (41) Hemorrhage, Chronic 1 [3.0] Inflammation, Acute 1 [3.0] Inflammation, Chronic 1 [2.0] Intestine Large, Rectum (46) (48) (50) (50) Artery, Inflammation, Chronic Active 1 [3.0] Liver (49) (50) (50) (50) Angiectasis 1 [1.0] Basophilic Focus 4 2 2 Clear Cell Focus 4 2 3 Eosinophilic Focus 12 8 3 4 Fatty Change 2 [2.0] 1 [2.0] Hematopoietic Cell Proliferation 1 [4.0] 1 [3.0] 1 [2.0] Infarct 1 Inflammation, Acute 1 [3.0] Inflammation, Chronic 1 [2.0] Inflammation, Granulomatous 1 [3.0] 1 [1.0] Mixed Cell Focus 1 Necrosis 1 [2.0] 3 [2.0] 1 [3.0] Tension Lipidosis 1 [2.0] 5 [1.4] 2 [1.5] 4 [1.5] Vacuolization Cytoplasmic, Focal 1 [3.0] Centrilobular, Hypertrophy 1 [3.0] Mesentery (17) (16) (4) (5) Artery, Inflammation, Chronic Active 1 [2.0] Fat, Congestion 1 [2.0] Fat, Hemorrhage 1 [2.0] Fat, Necrosis 17 [2.0] 15 [2.0] 3 [2.0] 5 [2.0] a Number of animals examined microscopically at site and number of animals with lesion b Average severity grade (1-minimal;2-mild;3-moderate;4-marked) Page 2 NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT18 Study Type: CHRONIC WITH AVERAGE SEVERITY GRADES[b] Date: 08/14/03 Route: RESPIRATORY EXPOSURE WHOLE BODY DIVINYLBENZENE Time: 13:13:26 ____________________________________________________________________________________________________________________________________ B6C3F1 MICE FEMALE CONTROL 10 PPM 30 PPM 100 PPM ____________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM - CONT Pancreas (48) (50) (50) (50) Atrophy 2 [4.0] 1 [4.0] Basophilic Focus 1 [2.0] Duct, Cyst 2 [4.0] Stomach, Forestomach (50) (50) (50) (50) Hyperplasia, Squamous 3 [1.7] 5 [2.2] 1 [2.0] Inflammation, Chronic Active 1 [1.0] 2 [2.0] Ulcer 1 [2.0] 1 [1.0] Stomach, Glandular (49) (50) (49) (49) Hemorrhage 1 [2.0] Necrosis 1 [2.0] Tooth (9) (13) (8) (15) Incisor, Dysplasia 9 [1.7] 13 [1.7] 8 [1.5] 15 [1.7] ____________________________________________________________________________________________________________________________________ CARDIOVASCULAR SYSTEM Blood Vessel (1) Aorta, Mineralization 1 [2.0] Heart (50) (50) (50) (50) Cardiomyopathy 3 [1.0] 6 [1.0] 1 [1.0] 1 [1.0] Infiltration Cellular, Polymorphonuclear 1 [2.0] Inflammation, Suppurative 1 [2.0] Mineralization 1 [1.0] 1 [2.0] 1 [1.0] Necrosis 1 [2.0] Thrombosis 1 [3.0] Artery, Inflammation, Chronic Active 2 [2.0] ____________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM Adrenal Cortex (50) (50) (50) (50) Hematopoietic Cell Proliferation 1 [3.0] Hyperplasia 1 [3.0] 2 [2.0] 5 [2.2] 4 [2.0] Hypertrophy 3 [2.0] 2 [1.0] 8 [2.0] Adrenal Medulla (49) (50) (49) (50) Hyperplasia 1 [2.0] 1 [2.0] 2 [2.0] Necrosis 1 [2.0] Islets, Pancreatic (48) (49) (49) (50) Hyperplasia 1 [3.0] Pituitary Gland (47) (50) (49) (45) Cyst 1 [2.0] Pars Distalis, Angiectasis 3 [2.0] a Number of animals examined microscopically at site and number of animals with lesion b Average severity grade (1-minimal;2-mild;3-moderate;4-marked) Page 3 NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT18 Study Type: CHRONIC WITH AVERAGE SEVERITY GRADES[b] Date: 08/14/03 Route: RESPIRATORY EXPOSURE WHOLE BODY DIVINYLBENZENE Time: 13:13:26 ____________________________________________________________________________________________________________________________________ B6C3F1 MICE FEMALE CONTROL 10 PPM 30 PPM 100 PPM ____________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - CONT Pars Distalis, Hyperplasia 8 [2.4] 16 [2.1] 6 [1.7] 5 [2.2] Thyroid Gland (49) (49) (50) (48) C-Cell, Hyperplasia 1 [1.0] Follicular Cell, Hyperplasia 1 [2.0] 2 [3.0] 1 [1.0] ____________________________________________________________________________________________________________________________________ GENERAL BODY SYSTEM None ____________________________________________________________________________________________________________________________________ GENITAL SYSTEM Ovary (48) (50) (49) (49) Angiectasis 2 [2.5] 3 [2.7] Cyst 9 [2.1] 14 [2.5] 10 [2.6] 9 [2.2] Inflammation, Acute 1 [4.0] Mineralization 1 [2.0] Necrosis 1 [4.0] Thrombosis 1 [3.0] Uterus (49) (50) (50) (49) Angiectasis 1 [3.0] 2 [3.0] 1 [4.0] Endometrium, Hyperplasia, Cystic 2 [3.0] 8 [3.8] 5 [2.8] 8 [3.3] ____________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM Lymph Node (9) (3) (5) (2) Ectasia 1 [3.0] Deep Cervical, Hyperplasia, Lymphoid 1 [4.0] Deep Cervical, Infiltration Cellular, Plasma Cell 1 [3.0] Iliac, Angiectasis 1 [3.0] Iliac, Ectasia 1 [3.0] Lumbar, Angiectasis 1 [3.0] Renal, Angiectasis 1 [3.0] Lymph Node, Mesenteric (49) (50) (49) (49) Angiectasis 1 [3.0] Ectasia 1 [3.0] Inflammation, Granulomatous 1 [2.0] Spleen (49) (50) (49) (49) Hematopoietic Cell Proliferation 3 [2.7] 3 [3.0] 3 [2.7] 1 [3.0] Inflammation, Acute 1 [3.0] a Number of animals examined microscopically at site and number of animals with lesion b Average severity grade (1-minimal;2-mild;3-moderate;4-marked) Page 4 NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT18 Study Type: CHRONIC WITH AVERAGE SEVERITY GRADES[b] Date: 08/14/03 Route: RESPIRATORY EXPOSURE WHOLE BODY DIVINYLBENZENE Time: 13:13:26 ____________________________________________________________________________________________________________________________________ B6C3F1 MICE FEMALE CONTROL 10 PPM 30 PPM 100 PPM ____________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - CONT Necrosis 1 [3.0] Sinusoid, Dilatation 1 [3.0] ____________________________________________________________________________________________________________________________________ INTEGUMENTARY SYSTEM Skin (50) (50) (50) (50) Inflammation, Chronic Active 3 [1.7] 2 [3.5] 1 [4.0] ____________________________________________________________________________________________________________________________________ MUSCULOSKELETAL SYSTEM None ____________________________________________________________________________________________________________________________________ NERVOUS SYSTEM Brain (50) (50) (50) (50) Degeneration, Focal 1 [2.0] Hemorrhage 1 [2.0] Meninges, Infiltration Cellular, Mononuclear Cell 1 [1.0] 1 [2.0] ____________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM Larynx (48) (50) (50) (49) Hyperplasia, Squamous 1 [2.0] Inflammation, Suppurative 1 [2.0] 1 [1.0] Lung (50) (50) (50) (49) Hemorrhage 1 [1.0] Infiltration Cellular, Polymorphonuclear 1 [2.0] Inflammation, Acute 1 [2.0] Thrombosis 1 [2.0] Alveolar Epithelium, Hyperplasia 4 [1.8] 3 [1.7] 4 [2.3] 8 [2.5] Alveolus, Granuloma 1 [1.0] Alveolus, Infiltration Cellular, Histiocyte 3 [1.0] 6 [1.0] 9 [1.1] 17 [1.2] Bronchiole, Hyperplasia 1 [4.0] Bronchiole, Hyperplasia, Atypical 39 [1.3] 45 [1.8] 48 [2.1] Nose (50) (50) (50) (49) Inflammation, Suppurative 1 [1.0] 50 [1.7] 49 [2.0] 49 [2.4] Glands, Respiratory Epithelium, Metaplasia 3 [1.0] 50 [3.1] 50 [3.6] 49 [4.0] Olfactory Epithelium, Atrophy 8 [1.1] a Number of animals examined microscopically at site and number of animals with lesion b Average severity grade (1-minimal;2-mild;3-moderate;4-marked) Page 5 NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT18 Study Type: CHRONIC WITH AVERAGE SEVERITY GRADES[b] Date: 08/14/03 Route: RESPIRATORY EXPOSURE WHOLE BODY DIVINYLBENZENE Time: 13:13:26 ____________________________________________________________________________________________________________________________________ B6C3F1 MICE FEMALE CONTROL 10 PPM 30 PPM 100 PPM ____________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - CONT Olfactory Epithelium, Degeneration, Hyaline 2 [1.5] 50 [2.4] 40 [1.8] 8 [1.6] Olfactory Epithelium, Respiratory Epithelium, Metaplasia 50 [3.1] 50 [3.9] 49 [3.9] Trachea (49) (50) (50) (50) Inflammation, Suppurative 1 [2.0] ____________________________________________________________________________________________________________________________________ SPECIAL SENSES SYSTEM Eye (50) (50) (50) (49) Cataract 1 [3.0] 2 [2.5] 1 [2.0] Inflammation 1 [2.0] Phthisis Bulbi 1 [4.0] 1 [3.0] Cornea, Hyperplasia, Squamous 1 [2.0] Cornea, Inflammation, Chronic Active 1 [2.0] 3 [2.3] 3 [3.0] Cornea, Inflammation, Suppurative 1 [3.0] Cornea, Mineralization 6 [1.0] Harderian Gland (50) (50) (50) (50) Hyperplasia 1 [3.0] 4 [2.5] 2 [2.0] 3 [2.3] Inflammation, Chronic Active 1 [4.0] ____________________________________________________________________________________________________________________________________ URINARY SYSTEM Kidney (49) (50) (50) (50) Amyloid Deposition 1 [3.0] Infarct 1 [4.0] Inflammation, Suppurative 2 [2.5] 2 [3.5] Metaplasia, Osseous 2 [1.5] 3 [1.3] 3 [1.0] 1 [1.0] Mineralization 1 [1.0] Nephropathy 25 [1.3] 31 [1.3] 22 [1.2] 17 [1.1] Artery, Inflammation, Chronic Active 1 [2.0] Urinary Bladder (49) (50) (50) (49) Inflammation, Suppurative 1 [2.0] Artery, Inflammation, Chronic Active 1 [2.0] a Number of animals examined microscopically at site and number of animals with lesion b Average severity grade (1-minimal;2-mild;3-moderate;4-marked) Page 6 NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT18 Study Type: CHRONIC WITH AVERAGE SEVERITY GRADES[b] Date: 08/14/03 Route: RESPIRATORY EXPOSURE WHOLE BODY DIVINYLBENZENE Time: 13:13:26 ____________________________________________________________________________________________________________________________________ B6C3F1 MICE MALE CONTROL 10 PPM 30 PPM 100 PPM ____________________________________________________________________________________________________________________________________ DISPOSITION SUMMARY Animals Initially In Study 50 50 50 50 Early Deaths Moribund Sacrifice 7 6 5 4 Natural Death 2 6 3 3 Survivors Terminal Sacrifice 41 38 42 43 Animals Examined Microscopically 50 50 50 50 ____________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM Liver (50) (50) (50) (50) Angiectasis 1 [1.0] Basophilic Focus 3 6 7 4 Clear Cell Focus 9 11 6 5 Eosinophilic Focus 8 7 2 Fatty Change 2 [1.5] 1 [2.0] Infarct 2 1 Inflammation, Granulomatous 1 [1.0] Mineralization 1 [1.0] Mixed Cell Focus 2 1 2 1 Necrosis 1 [1.0] 2 [1.5] 2 [3.0] 2 [3.0] Tension Lipidosis 2 [2.0] 1 [2.0] 2 [1.5] 1 [2.0] Vacuolization Cytoplasmic, Focal 2 [2.5] Mesentery (11) (4) (4) (5) Fat, Necrosis 10 [2.0] 4 [2.0] 4 [2.0] 5 [2.0] Pancreas (49) (48) (50) (50) Duct, Cyst 2 [3.5] Stomach, Forestomach (49) (50) (49) (50) Hyperplasia, Squamous 1 [2.0] 4 [2.5] 2 [1.0] 4 [2.3] Inflammation 1 [2.0] Inflammation, Acute 2 [1.5] 1 [1.0] Inflammation, Chronic Active 1 [2.0] 1 [2.0] 3 [2.3] Mineralization 1 [1.0] Ulcer 2 [2.5] 1 [2.0] 1 [3.0] Stomach, Glandular (48) (48) (47) (49) Inflammation, Acute 1 [1.0] Mineralization 1 [2.0] Necrosis 1 [2.0] 1 [1.0] Tooth (41) (43) (41) (35) Incisor, Dysplasia 41 [2.2] 43 [2.6] 41 [2.5] 35 [2.3] a Number of animals examined microscopically at site and number of animals with lesion b Average severity grade (1-minimal;2-mild;3-moderate;4-marked) Page 7 NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT18 Study Type: CHRONIC WITH AVERAGE SEVERITY GRADES[b] Date: 08/14/03 Route: RESPIRATORY EXPOSURE WHOLE BODY DIVINYLBENZENE Time: 13:13:26 ____________________________________________________________________________________________________________________________________ B6C3F1 MICE MALE CONTROL 10 PPM 30 PPM 100 PPM ____________________________________________________________________________________________________________________________________ CARDIOVASCULAR SYSTEM Heart (50) (49) (50) (50) Cardiomyopathy 14 [1.1] 5 [1.0] 5 [1.0] 5 [1.0] Mineralization 1 [1.0] 1 [1.0] 1 [1.0] Artery, Inflammation, Chronic Active 1 [3.0] ____________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM Adrenal Cortex (49) (49) (50) (50) Hyperplasia 9 [2.0] 10 [1.9] 6 [1.7] 5 [2.0] Hypertrophy 24 [2.0] 25 [1.9] 26 [2.0] 24 [1.6] Adrenal Medulla (49) (49) (50) (50) Hyperplasia 1 [2.0] 2 [2.0] 2 [2.5] Parathyroid Gland (35) (39) (36) (35) Hyperplasia 1 [3.0] Pituitary Gland (48) (47) (47) (46) Cyst 1 [3.0] Pars Distalis, Hyperplasia 2 [2.5] 1 [2.0] 2 [1.5] 1 [1.0] Thyroid Gland (49) (49) (50) (50) Follicular Cell, Hyperplasia 2 [1.5] 1 [3.0] ____________________________________________________________________________________________________________________________________ GENERAL BODY SYSTEM None ____________________________________________________________________________________________________________________________________ GENITAL SYSTEM Epididymis (50) (50) (50) (50) Granuloma Sperm 1 [2.0] 2 [1.5] Penis (1) Inflammation, Acute 1 [4.0] Preputial Gland (50) (50) (50) (50) Ectasia 1 [2.0] 3 [2.3] 2 [3.0] 1 [3.0] Inflammation, Chronic Active 2 [3.0] Seminal Vesicle (49) (48) (50) (49) Inflammation, Chronic 1 [3.0] Testes (50) (50) (50) (50) Atrophy 2 [3.0] Mineralization 1 [4.0] a Number of animals examined microscopically at site and number of animals with lesion b Average severity grade (1-minimal;2-mild;3-moderate;4-marked) Page 8 NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT18 Study Type: CHRONIC WITH AVERAGE SEVERITY GRADES[b] Date: 08/14/03 Route: RESPIRATORY EXPOSURE WHOLE BODY DIVINYLBENZENE Time: 13:13:26 ____________________________________________________________________________________________________________________________________ B6C3F1 MICE MALE CONTROL 10 PPM 30 PPM 100 PPM ____________________________________________________________________________________________________________________________________ GENITAL SYSTEM - CONT Interstitial Cell, Hyperplasia 1 [2.0] ____________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM Bone Marrow (50) (48) (50) (50) Necrosis 1 [4.0] Spleen (49) (48) (50) (50) Hematopoietic Cell Proliferation 1 [3.0] 1 [2.0] ____________________________________________________________________________________________________________________________________ INTEGUMENTARY SYSTEM Skin (50) (50) (50) (50) Cyst Epithelial Inclusion 1 [3.0] Inflammation, Chronic Active 3 [2.0] 1 [1.0] 2 [3.5] Inflammation, Granulomatous 1 [2.0] Subcutaneous Tissue, Cyst Epithelial Inclusion 1 ____________________________________________________________________________________________________________________________________ MUSCULOSKELETAL SYSTEM None ____________________________________________________________________________________________________________________________________ NERVOUS SYSTEM Brain (50) (50) (50) (50) Necrosis, Focal 1 [2.0] Artery, Inflammation, Chronic Active 1 [2.0] ____________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM Larynx (50) (50) (50) (50) Inflammation, Suppurative 1 [3.0] Lung (49) (49) (49) (49) Hemorrhage 1 [2.0] 1 [4.0] Mineralization 1 [2.0] Alveolar Epithelium, Hyperplasia 1 [1.0] 5 [3.2] 5 [2.0] 7 [2.0] Alveolus, Infiltration Cellular, Histiocyte 2 [1.5] 4 [1.0] 5 [1.0] 1 [1.0] Bronchiole, Hyperplasia 1 [1.0] a Number of animals examined microscopically at site and number of animals with lesion b Average severity grade (1-minimal;2-mild;3-moderate;4-marked) Page 9 NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT18 Study Type: CHRONIC WITH AVERAGE SEVERITY GRADES[b] Date: 08/14/03 Route: RESPIRATORY EXPOSURE WHOLE BODY DIVINYLBENZENE Time: 13:13:26 ____________________________________________________________________________________________________________________________________ B6C3F1 MICE MALE CONTROL 10 PPM 30 PPM 100 PPM ____________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - CONT Bronchiole, Hyperplasia, Atypical 38 [1.1] 46 [1.8] 46 [1.9] Bronchiole, Inflammation, Chronic Active 1 [3.0] Nose (50) (50) (49) (50) Inflammation, Suppurative 3 [1.0] 47 [1.4] 49 [1.9] 49 [1.9] Glands, Necrosis 1 [2.0] Glands, Respiratory Epithelium, Metaplasia 12 [1.0] 50 [2.9] 49 [4.0] 50 [3.9] Olfactory Epithelium, Atrophy 14 [1.1] Olfactory Epithelium, Degeneration, Hyaline 5 [1.0] 50 [1.9] 48 [1.8] 11 [1.1] Olfactory Epithelium, Respiratory Epithelium, Metaplasia 1 [2.0] 50 [3.1] 49 [4.0] 50 [3.9] Respiratory Epithelium, Metaplasia, Squamous 1 [2.0] Sinus, Foreign Body 1 ____________________________________________________________________________________________________________________________________ SPECIAL SENSES SYSTEM Eye (49) (47) (47) (50) Phthisis Bulbi 1 [4.0] 1 [3.0] Cornea, Inflammation, Chronic Active 2 [2.0] 1 [3.0] 1 [2.0] Cornea, Mineralization 2 [1.0] Harderian Gland (50) (49) (50) (50) Hyperplasia 2 [2.0] 2 [2.0] 3 [2.7] 2 [1.5] ____________________________________________________________________________________________________________________________________ URINARY SYSTEM Kidney (50) (50) (50) (50) Cyst 1 [2.0] 2 [2.0] Infarct 1 [3.0] 2 [2.5] 1 [2.0] Inflammation, Suppurative 1 [2.0] Metaplasia, Osseous 7 [1.0] 3 [1.0] 5 [1.0] 1 [1.0] Nephropathy 45 [1.6] 43 [1.5] 40 [1.3] 34 [1.3] Capsule, Fibrosis 1 [1.0] Papilla, Necrosis 1 [2.0] Pelvis, Dilatation 1 [2.0] Renal Tubule, Hyperplasia 2 [1.0] 1 [1.0] Renal Tubule, Necrosis 1 [2.0] Urinary Bladder (48) (48) (49) (49) Transitional Epithelium, Hyperplasia 1 [3.0] a Number of animals examined microscopically at site and number of animals with lesion b Average severity grade (1-minimal;2-mild;3-moderate;4-marked) Page 10 ------------------------------------------------------------ ---------- END OF REPORT ---------- ------------------------------------------------------------