National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/refchem

NICEATM Reference Chemical Lists for Test Method Evaluations

Below are lists of reference chemicals that may be useful for development or validation of new test methods. Some lists were developed during studies on new test methods and approaches conducted by NICEATM; others have been recommended by ICCVAM for validation of alternative test methods. Lists are downloadable as both Excel spreadsheets and tab-delimited text files. They are accompanied by links to the ICCVAM Test Method Evaluation Reports or references in the scientific literature in which they were originally published, as well as links to pages on the NICEATM website that have additional information about the context under which the lists were developed. Users of these lists are encouraged to consult these references to help them decide whether any of these lists might be useful for a specific test method development activity.

Endocrine Disruptors

Androgen Receptor Agonist and Antagonist Chemicals

Published as Table 2 in:
Kleinstreuer et al. 2016. Development and validation of a computational model for androgen receptor activity [published online ahead of print 18 Nov 2016]. Chem Res Toxicol.

Estrogen Receptor Agonist Chemicals

Published as Tables 2 and 3 in:
Browne et al. 2015. Screening chemicals for estrogen receptor bioactivity using a computational model. Environ Sci Tech 49(14):8804-8814.

Curated Database of Rodent Uterotrophic Bioactivity

NICEATM compiled data from the peer-reviewed literature for in vivo uterotrophic assays that met six minimum criteria specified by accepted test guidelines. These data have potential utility for developing adverse outcome pathways or models of estrogenic activity, prioritizing chemicals for further testing, or evaluating species-specific responses to chemicals.

NICEATM Guideline-like Uterotrophic Database

More information about the Uterotrophic Database

Skin Sensitization

Recommended Reference Substances for the Murine Local Lymph Node Assay (LLNA)

Published as Appendix F of Recommended Performance Standards: Murine Local Lymph Node Assay, NIH Document No. 09-7357, 2010

NICEATM LLNA Database

Data from analyses of the LLNA conducted on behalf of ICCVAM by NICEATM are available to interested stakeholders as a reference for developing and evaluating alternative approaches to testing and assessment that replace, reduce, or refine the use of animals for identification of potential skin sensitizers.

NICEATM LLNA database

More information about ICCVAM evaluations of the LLNA and other skin sensitization test methods

Ocular Corrosion and Irritation

Recommended Substances for Optimization or Validation of In Vitro Ocular Toxicity Test Methods

Published as Appendix H in ICCVAM Test Method Evaluation Report, NIH Document No. 07-4517, 2006
More information about ICCVAM evaluations of in vitro ocular toxicity test methods:

Acute Systemic Toxicity
Recommended Reference Substances for Evaluation of In Vitro Basal Cytotoxicity Methods for Predicting the Starting Dose for Rodent Acute Oral Toxicity Tests

Published as Table 3-1 in ICCVAM Test Method Evaluation Report, NIH Document No. 07-4519, 2006
More information about the NICEATM validation study of in vitro cytotoxicity test methods

Skin Corrosion and Irritation

Recommended Chemicals for Validation of New In Vitro Skin Corrosivity Test Methods

Published in ICCVAM Recommended Performance Standards for In Vitro Dermal Corrosivity Test Methods, NIH Document No. 04-4510, 2004
More information about ICCVAM evaluations of test methods to identify dermal corrosives

NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.