Abstract for TER90031

Developmental Toxicology of California Pesticide/Fertilizer Mixture in Sprague Dawley (CD) Rats

CASRN: PestFertMix2
Report Date: February 1992


The following abstract presents results of a study conducted by a contract laboratory for the National Toxicology Program. The findings may not have been peer reviewed and were not evaluated in accordance with the levels of evidence criteria established by NTP in March 2009. For more information, see the Explanation of Levels of Evidence for Developmental Toxicity. The findings and conclusions for this study should not be construed to represent the views of NTP or the U.S. Government.

Pesticides and fertilizers, as used in modern agriculture, contribute to the overall low-level contamination of groundwater sources. In order to determine the potential of pesticide and fertilizer mixtures to produce developmental toxicity at concentrations up to l00 times those found in groundwater, we studied a mixture of five pesticides (aldicarb, atrazine, dibromochloropropane, ethylene dibromide, and simazine) and one fertilizer component (ammonium nitrate).

These chemicals and their relative concentrations in the stock mixture were selected on the basis of survey data from California (pesticides) and Iowa (fertilizer). The mixture CALF was administered in the drinking water to Sprague-Dawley rats (21-23 per group) on gestational days 6 to 20 at three dose levels, i.e., lX, l0X, and l00X, where lX was the median concentration of each pesticide component as determined in the surveys. Dams were monitored daily for signs of toxicity. On gd 20 fetuses were removed and examined for effects of CALF on growth, viability, and morphological development.

Maternal body weights, food and water consumption and clinical signs were all similar to the control values. No adverse effects of CALF treatment were observed for measures of embryo/fetal toxicity, including resorptions per litter, live litter size, and fetal body weight. CALF did not cause an increased incidence of malformations or variations.

In summary, under the conditions of this study, exposure of pregnant rats to a mixture of ammonium nitrate and pesticides at levels up to l00-fold greater than the median human exposure in groundwater supplies did not show any detectable adverse effects on the dam or developing conceptus.