Abstract for RACB90027

Reproductive Toxicity of Pesticide/Fertilizer Mixture II in CD-1 Swiss Mice

Report Date: January 1992


The following abstract presents results of a study conducted by a contract laboratory for the National Toxicology Program. The findings were not evaluated in accordance with the levels of evidence for reproductive or developmental criteria established by NTP in March 2009. The findings and conclusions for this study should not be construed to represent the views of NTP or the U.S. Government.

Pesticide/Fertilizer Mixture II (PFM II) is composed of six pesticides (Aldicarb, Atrazine, Dibromochloropropane, 1,2- Dichloropropane, Ethylene Dibromide, and Simazine) and 1 fertilizer (Ammonium nitrate) and represents a real-life mixture of ground water contamination in California. PFM II in drinking water was tested for its effects on fertility and reproduction in Swiss CD-l mice according to the Continuous Breeding Protocol. 0, 1, 10, and 100X (1X being the median concentration found in ground water) were chosen to investigate the effects on fertility and reproduction.

Male and female mice (F0) were continuously exposed for a 7- day precohabitation and a 98-day cohabitation period (Task 2). Male and female body weights in Task 2 were within 10% of control values. Water consumption was similar in F0 control and treated animals. Fertility and reproduction in F0 animals were not effected by PFM II.

The F1 pups from the final litter in the control, 10 and 100X groups were weaned for second generation studies. At necropsy, kidney/adrenal weights were approximately 10% higher than controls in F1 10X-treated males and females and 100X-treated females. Absolute seminal vesicle weight as well as seminal vesicle-to-body weight ratio significantly decreased (ca. 11%) in the 100X dose group. PFM II produced mild systemic toxicity and no reproductive toxicity in Swiss CD-l mice at the dose levels tested.

NTIS # PB92-140730/AS