TDMS Study 99034-01 Pathology Tables
NTP Experiment-Test: 99034-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT18
Study Type: SUBCHRON 26-WEEK WITH AVERAGE SEVERITY GRADES[b] Date: 11/05/03
Route: GAVAGE TRANSGENIC MODEL EVALUATION II (BENZENE) Time: 15:01:19
FINAL #1 MICE
Facility: Battelle Columbus Laboratory
Chemical CAS #: 71-43-2
Lock Date: 10/15/01
Cage Range: All
Reasons For Removal: All
Removal Date Range: All
Treatment Groups: Include All
a Number of animals examined microscopically at site and number of animals with lesion
b Average severity grade (1-minimal;2-mild;3-moderate;4-marked)
Page 1
NTP Experiment-Test: 99034-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT18
Study Type: SUBCHRON 26-WEEK WITH AVERAGE SEVERITY GRADES[b] Date: 11/05/03
Route: GAVAGE TRANSGENIC MODEL EVALUATION II (BENZENE) Time: 15:01:19
____________________________________________________________________________________________________________________________________
P16(INK4A)/(+/-) (C57BL/6) FEMALE 0 MG/KG 25 MG/KG 50 MG/KG 100 200
MG/KG MG/KG
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 15 15 15 15 15
Early Deaths
Survivors
Terminal Sacrifice 15 15 15 15 15
Animals Examined Microscopically 15 15 15 15 15
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Intestine Small, Duodenum (15) (15) (15) (15) (15)
Inflammation, Chronic Active 2 [1.0]
Liver (15) (15) (15) (15) (15)
Hematopoietic Cell Proliferation 1 [2.0] 1 [1.0]
Inflammation, Chronic 10 [1.0] 6 [1.0] 6 [1.0] 4 [1.0] 11 [1.0]
Stomach, Forestomach (15) (15) (15) (15) (15)
Erosion 1 [2.0]
Hyperplasia 1 [1.0]
Inflammation, Chronic 3 [2.0]
Stomach, Glandular (15) (15) (15) (15) (15)
Mineralization 1 [1.0]
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (15) (15) (15) (15) (15)
Myocardium, Inflammation, Chronic 1 [1.0]
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Pituitary Gland (15) (15) (15) (15) (15)
Infiltration Cellular, Lymphoid 1 [1.0]
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
a Number of animals examined microscopically at site and number of animals with lesion
b Average severity grade (1-minimal;2-mild;3-moderate;4-marked)
Page 2
NTP Experiment-Test: 99034-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT18
Study Type: SUBCHRON 26-WEEK WITH AVERAGE SEVERITY GRADES[b] Date: 11/05/03
Route: GAVAGE TRANSGENIC MODEL EVALUATION II (BENZENE) Time: 15:01:19
____________________________________________________________________________________________________________________________________
P16(INK4A)/(+/-) (C57BL/6) FEMALE 0 MG/KG 25 MG/KG 50 MG/KG 100 200
MG/KG MG/KG
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM - CONT
Clitoral Gland (1)
Pigmentation 1 [3.0]
Ovary (14) (15) (15) (15) (15)
Angiectasis 1 [1.0]
Cyst 1 [1.0] 2 [1.0] 2 [1.0]
Uterus (15) (15) (15) (15) (15)
Inflammation, Chronic Active 1 [1.0]
Inflammation, Suppurative 1 [1.0] 1 [1.0] 1 [1.0]
Endometrium, Hyperplasia, Cystic 12 [1.0] 15 [1.1] 15 [1.0] 15 [1.1] 14 [1.1]
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Lymph Node (13) (11) (11) (12) (13)
Lumbar, Hyperplasia, Lymphoid 1 [3.0]
Lumbar, Pigmentation 1 [2.0]
Mediastinal, Atrophy 2 [1.5] 4 [1.3] 2 [1.0]
Lymph Node, Mandibular (14) (15) (15) (15) (15)
Atrophy 1 [1.0] 2 [1.0] 4 [1.0]
Hyperplasia, Lymphoid 1 [1.0] 4 [1.3] 2 [1.5] 5 [1.4] 3 [1.0]
Infiltration Cellular, Polymorphonuclear 1 [1.0] 2 [1.0]
Lymph Node, Mesenteric (15) (15) (15) (15) (15)
Atrophy 2 [2.0] 3 [1.0] 8 [1.8] 6 [1.3]
Hyperplasia, Lymphoid 1 [2.0]
Spleen (15) (15) (15) (15) (15)
Hematopoietic Cell Proliferation 5 [1.0] 5 [1.2] 2 [1.0] 3 [1.3] 7 [1.1]
Infiltration Cellular, Lymphocyte 1 [4.0]
Lymphoid Follicle, Atrophy 1 [1.0] 1 [2.0] 1 [2.0]
Thymus (15) (15) (15) (15) (15)
Hyperplasia, Atypical 1 [2.0] 1 [4.0] 1 [3.0] 1 [2.0]
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Mammary Gland (15) (15) (15) (15) (15)
Hyperplasia 2 [1.0]
Skin (15) (15) (15) (15) (15)
Inflammation, Granulomatous 1 [1.0] 1 [2.0]
Inflammation, Suppurative 1 [1.0] 1 [2.0]
Pigmentation 1 [1.0] 8 [1.0] 15 [1.1] 15 [1.1]
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
b Average severity grade (1-minimal;2-mild;3-moderate;4-marked)
Page 3
NTP Experiment-Test: 99034-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT18
Study Type: SUBCHRON 26-WEEK WITH AVERAGE SEVERITY GRADES[b] Date: 11/05/03
Route: GAVAGE TRANSGENIC MODEL EVALUATION II (BENZENE) Time: 15:01:19
____________________________________________________________________________________________________________________________________
P16(INK4A)/(+/-) (C57BL/6) FEMALE 0 MG/KG 25 MG/KG 50 MG/KG 100 200
MG/KG MG/KG
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (15) (15) (15) (15) (15)
Fibrosis 1 [1.0] 1 [1.0] 1 [1.0]
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
None
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (15) (15) (15) (15) (15)
Inflammation, Chronic Active 2 [1.5] 2 [1.0] 2 [1.0]
Alveolar Epithelium, Hyperplasia, Focal 1 [1.0] 3 [1.3]
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
None
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (15) (15) (15) (15) (15)
Casts Protein 5 [1.0] 4 [1.0] 5 [1.0] 8 [1.0] 7 [1.0]
Infiltration Cellular, Lymphoid 1 [1.0]
Infiltration Cellular, Lymphocyte 1 [2.0]
Nephropathy 3 [1.0] 1 [1.0] 2 [1.0] 1 [1.0]
a Number of animals examined microscopically at site and number of animals with lesion
b Average severity grade (1-minimal;2-mild;3-moderate;4-marked)
Page 4
NTP Experiment-Test: 99034-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT18
Study Type: SUBCHRON 26-WEEK WITH AVERAGE SEVERITY GRADES[b] Date: 11/05/03
Route: GAVAGE TRANSGENIC MODEL EVALUATION II (BENZENE) Time: 15:01:19
____________________________________________________________________________________________________________________________________
P16(INK4A)/(+/-) (C57BL/6) MALE 0 MG/KG 25 MG/KG 50 MG/KG 100 200
MG/KG MG/KG
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 15 15 15 15 15
Early Deaths
Moribund Sacrifice 1
Survivors
Terminal Sacrifice 15 15 15 15 14
Animals Examined Microscopically 15 15 15 15 15
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Liver (15) (15) (15) (15) (15)
Inflammation, Chronic 3 [1.0] 6 [1.0] 1 [1.0] 3 [1.0] 1 [1.0]
Salivary Glands (1) (1)
Submandibular Gland, Atrophy 1 [2.0]
Submandibular Gland, Inflammation, Chronic 1 [1.0]
Stomach, Forestomach (15) (15) (15) (15) (15)
Erosion 1 [3.0]
Hyperplasia 1 [1.0] 3 [2.0]
Inflammation, Acute 1 [2.0]
Stomach, Glandular (15) (15) (15) (15) (15)
Mineralization 1 [1.0]
Glands, Cyst 1 [1.0] 1 [1.0]
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (15) (15) (15) (15) (15)
Myocardium, Inflammation, Chronic 1 [1.0] 1 [1.0]
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
None
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
b Average severity grade (1-minimal;2-mild;3-moderate;4-marked)
Page 5
NTP Experiment-Test: 99034-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT18
Study Type: SUBCHRON 26-WEEK WITH AVERAGE SEVERITY GRADES[b] Date: 11/05/03
Route: GAVAGE TRANSGENIC MODEL EVALUATION II (BENZENE) Time: 15:01:19
____________________________________________________________________________________________________________________________________
P16(INK4A)/(+/-) (C57BL/6) MALE 0 MG/KG 25 MG/KG 50 MG/KG 100 200
MG/KG MG/KG
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Epididymis (15) (15) (15) (15) (15)
Granuloma Sperm 1 [2.0]
Unilateral, Hypospermia 1 [4.0]
Testes (15) (15) (15) (15) (15)
Germinal Epithelium, Atrophy 1 [2.0]
Germinal Epithelium, Syncytial Alteration 1 [1.0] 1 [1.0]
Rete Testes, Inflammation, Granulomatous 1 [2.0]
Unilateral, Germinal Epithelium, Atrophy 1 [2.0]
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (15) (15) (15) (15) (15)
Atrophy 10 [1.1] 12 [1.3]
Pigmentation, Hemosiderin 1 [1.0] 13 [1.0] 13 [1.1] 15 [2.0] 14 [2.9]
Lymph Node (11) (11) (12) (9) (7)
Mediastinal, Atrophy 2 [1.0] 6 [1.3] 6 [1.7]
Lymph Node, Mandibular (15) (15) (15) (14) (14)
Atrophy 6 [1.3] 11 [1.2]
Hyperplasia, Lymphoid 2 [1.0] 1 [1.0] 1 [1.0]
Infiltration Cellular, Polymorphonuclear 1 [2.0] 1 [1.0]
Lymph Node, Mesenteric (15) (15) (14) (15) (15)
Atrophy 1 [1.0] 2 [2.0] 2 [1.0] 13 [2.0] 13 [1.7]
Infiltration Cellular, Polymorphonuclear 1 [2.0]
Spleen (15) (15) (15) (15) (15)
Hematopoietic Cell Proliferation 1 [1.0] 2 [1.0] 8 [1.3]
Lymphoid Follicle, Atrophy 15 [1.8] 14 [2.9]
Thymus (15) (15) (15) (15) (15)
Atrophy 7 [3.0] 13 [2.9]
Mineralization 1 [1.0]
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Skin (15) (15) (15) (15) (15)
Pigmentation 15 [1.1] 15 [1.6] 15 [2.3] 14 [2.6]
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (15) (15) (15) (15) (15)
Pigmentation, Hemosiderin 1 [1.0]
a Number of animals examined microscopically at site and number of animals with lesion
b Average severity grade (1-minimal;2-mild;3-moderate;4-marked)
Page 6
NTP Experiment-Test: 99034-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT18
Study Type: SUBCHRON 26-WEEK WITH AVERAGE SEVERITY GRADES[b] Date: 11/05/03
Route: GAVAGE TRANSGENIC MODEL EVALUATION II (BENZENE) Time: 15:01:19
____________________________________________________________________________________________________________________________________
P16(INK4A)/(+/-) (C57BL/6) MALE 0 MG/KG 25 MG/KG 50 MG/KG 100 200
MG/KG MG/KG
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
None
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (15) (15) (15) (15) (15)
Inflammation, Chronic Active 2 [1.0] 1 [1.0] 3 [1.0] 5 [1.0]
Alveolar Epithelium, Hyperplasia, Focal 1 [1.0]
Bronchiole, Hyperplasia, Focal 1 [1.0]
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
None
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (15) (15) (15) (15) (15)
Casts Protein 1 [1.0] 2 [1.0] 1 [1.0] 2 [1.0]
Nephropathy 1 [1.0] 1 [1.0] 2 [1.0] 4 [1.3]
a Number of animals examined microscopically at site and number of animals with lesion
b Average severity grade (1-minimal;2-mild;3-moderate;4-marked)
Page 7
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