National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/19278

TDMS Study 96017-06 Pathology Tables

NTP Experiment-Test: 96017-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                         WATER DISINFECTION BYPRODUCTS (DIBROMOACETIC ACID)                    Date: 05/19/04
Route: DOSED WATER                                                                                                Time: 09:07:01

                                                          FINAL#1 MICE




       Facility:  Southern Research Institute

       Chemical CAS #:  631-64-1

       Lock Date:  11/15/02

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All


































a  Number of animals examined microscopically at site and number of animals with lesion

                                                              Page   1


NTP Experiment-Test: 96017-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                         WATER DISINFECTION BYPRODUCTS (DIBROMOACETIC ACID)                    Date: 05/19/04    
Route: DOSED WATER                                                                                                Time: 09:07:01    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/L       50 MG/L      500 MG/L     1000                                      
                                                                                          MG/L                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially In Study                          50           50           50           50                                     
  Early Deaths                                                                                                                      
    Natural Death                                     10           13           13           11                                     
    Moribund Sacrifice                                 2            2            5            7                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                38           35           31           32                                     
    Natural Death                                                                1                                                  
                                                                                                                                    
  Animals Examined Microscopically                    50           50           50           50                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Intestine Large, Rectum                            (49)         (49)         (48)         (49)                                   
      Ulcer                                                                      1 (2%)                                             
   Intestine Large, Cecum                             (47)         (46)         (49)         (46)                                   
      Edema                                            2 (4%)       1 (2%)                    3 (7%)                                
      Inflammation, Chronic                                         1 (2%)                    1 (2%)                                
      Epithelium, Hyperplasia                                                    1 (2%)                                             
   Intestine Small, Duodenum                          (46)         (48)         (48)         (45)                                   
      Epithelium, Hyperplasia                                       1 (2%)                                                          
   Intestine Small, Jejunum                           (46)         (47)         (49)         (43)                                   
      Hyperplasia, Lymphoid                                         2 (4%)                    2 (5%)                                
   Intestine Small, Ileum                             (45)         (47)         (47)         (46)                                   
      Inflammation, Chronic                                         1 (2%)                                                          
   Liver                                              (49)         (50)         (50)         (49)                                   
      Angiectasis                                      1 (2%)       2 (4%)                                                          
      Basophilic Focus                                              1 (2%)       3 (6%)       3 (6%)                                
      Clear Cell Focus                                 3 (6%)       1 (2%)       8 (16%)      5 (10%)                               
      Eosinophilic Focus                               7 (14%)      6 (12%)     10 (20%)      9 (18%)                               
      Hematopoietic Cell Proliferation                 2 (4%)       4 (8%)       2 (4%)       2 (4%)                                
      Hepatodiaphragmatic Nodule                                                              2 (4%)                                
      Hyperplasia, Lymphoid                            2 (4%)       1 (2%)                                                          
      Infarct                                                                                 1 (2%)                                
      Infiltration Cellular, Mixed Cell                7 (14%)      7 (14%)      4 (8%)       9 (18%)                               
      Inflammation, Chronic Active                                               1 (2%)                                             
      Mixed Cell Focus                                 2 (4%)       6 (12%)      3 (6%)       1 (2%)                                
      Necrosis, Focal                                  3 (6%)       2 (4%)       3 (6%)       2 (4%)                                
      Tension Lipidosis                                1 (2%)       3 (6%)                                                          
      Centrilobular, Necrosis                                       1 (2%)       4 (8%)       4 (8%)                                
      Hepatocyte, Vacuolization Cytoplasmic            2 (4%)       7 (14%)      4 (8%)       2 (4%)                                
      Kupffer Cell, Pigmentation                       3 (6%)                                 3 (6%)                                
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    2                                                              
                                                                                                                                   
NTP Experiment-Test: 96017-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                         WATER DISINFECTION BYPRODUCTS (DIBROMOACETIC ACID)                    Date: 05/19/04    
Route: DOSED WATER                                                                                                Time: 09:07:01    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/L       50 MG/L      500 MG/L     1000                                      
                                                                                          MG/L                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - CONT                                                                                                            
   Mesentery                                          (28)         (20)         (16)         (19)                                   
      Fat, Necrosis                                   22 (79%)     18 (90%)     11 (69%)     13 (68%)                               
   Pancreas                                           (49)         (49)         (49)         (47)                                   
      Atrophy                                                       1 (2%)       1 (2%)       3 (6%)                                
      Cyst                                                                       1 (2%)       3 (6%)                                
      Inflammation, Chronic                                                                   1 (2%)                                
      Acinus, Cytoplasmic Alteration                   1 (2%)                                                                       
   Salivary Glands                                    (50)         (49)         (50)         (50)                                   
      Hyperplasia, Lymphoid                           15 (30%)     13 (27%)     16 (32%)     10 (20%)                               
   Stomach, Forestomach                               (49)         (50)         (50)         (49)                                   
      Erosion                                                       1 (2%)                                                          
      Inflammation, Chronic                            1 (2%)       2 (4%)       2 (4%)       1 (2%)                                
      Ulcer                                            1 (2%)       1 (2%)       1 (2%)       3 (6%)                                
      Epithelium, Hyperplasia                          4 (8%)       5 (10%)                   4 (8%)                                
   Stomach, Glandular                                 (49)         (50)         (50)         (49)                                   
      Erosion                                                       1 (2%)                    1 (2%)                                
      Pylorus, Epithelium, Hyperplasia, Atypical                    1 (2%)                                                          
   Tooth                                                                        (1)                                                 
      Malformation                                                               1 (100%)                                           
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Blood Vessel                                       (1)          (1)          (1)                                                 
      Hypertrophy                                                                1 (100%)                                           
      Inflammation, Chronic Active                                               1 (100%)                                           
   Heart                                              (50)         (49)         (50)         (50)                                   
      Cardiomyopathy                                                             1 (2%)       1 (2%)                                
      Inflammation, Chronic                                         1 (2%)                    1 (2%)                                
      Inflammation, Chronic Active                                                            1 (2%)                                
      Mineralization                                                1 (2%)       1 (2%)       2 (4%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (50)         (50)         (50)         (49)                                   
      Accessory Adrenal Cortical Nodule                9 (18%)     11 (22%)      6 (12%)      9 (18%)                               
      Hyperplasia, Focal                                            2 (4%)                    1 (2%)                                
      Hypertrophy, Focal                                            1 (2%)                                                          
      Capsule, Hyperplasia                                          1 (2%)                    1 (2%)                                
   Adrenal Medulla                                    (50)         (50)         (50)         (49)                                   
      Hyperplasia                                                   2 (4%)       1 (2%)       1 (2%)                                
   Islets, Pancreatic                                 (49)         (49)         (49)         (46)                                   
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    3                                                              
                                                                                                                                   
NTP Experiment-Test: 96017-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                         WATER DISINFECTION BYPRODUCTS (DIBROMOACETIC ACID)                    Date: 05/19/04    
Route: DOSED WATER                                                                                                Time: 09:07:01    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/L       50 MG/L      500 MG/L     1000                                      
                                                                                          MG/L                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ENDOCRINE SYSTEM - CONT                                                                                                             
      Hyperplasia                                      2 (4%)       5 (10%)      3 (6%)       3 (7%)                                
   Parathyroid Gland                                  (45)         (49)         (47)         (49)                                   
      Cyst                                             2 (4%)       1 (2%)       1 (2%)       3 (6%)                                
   Pituitary Gland                                    (47)         (49)         (49)         (49)                                   
      Pars Distalis, Angiectasis                       2 (4%)       3 (6%)       1 (2%)       1 (2%)                                
      Pars Distalis, Cyst                              2 (4%)       2 (4%)       1 (2%)       1 (2%)                                
      Pars Distalis, Hyperplasia, Focal                4 (9%)       9 (18%)      4 (8%)       7 (14%)                               
      Pars Intermedia, Hyperplasia, Focal              1 (2%)                                                                       
   Thyroid Gland                                      (50)         (50)         (50)         (50)                                   
      Degeneration, Cystic                            10 (20%)     26 (52%)     16 (32%)     14 (28%)                               
      Follicular Cell, Hyperplasia                     8 (16%)      1 (2%)       1 (2%)       2 (4%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Clitoral Gland                                     (50)         (48)         (48)         (49)                                   
      Cyst                                                          1 (2%)                                                          
   Ovary                                              (49)         (47)         (47)         (49)                                   
      Angiectasis                                      5 (10%)      3 (6%)       9 (19%)      7 (14%)                               
      Cyst                                            14 (29%)     12 (26%)     14 (30%)     22 (45%)                               
      Corpus Luteum, Hyperplasia                       1 (2%)       1 (2%)                                                          
      Granulosa Cell, Hyperplasia                      1 (2%)                                                                       
      Interstitial Cell, Hyperplasia                                                          1 (2%)                                
   Uterus                                             (50)         (50)         (50)         (50)                                   
      Angiectasis                                                   4 (8%)       1 (2%)       2 (4%)                                
      Hyperplasia, Cystic                             44 (88%)     42 (84%)     44 (88%)     39 (78%)                               
      Inflammation, Chronic                            1 (2%)       1 (2%)       1 (2%)                                             
      Metaplasia, Squamous                             1 (2%)                    1 (2%)       1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (50)         (50)         (50)         (50)                                   
      Hyperplasia                                     20 (40%)     26 (52%)     24 (48%)     20 (40%)                               
      Infiltration Cellular, Mast Cell                              1 (2%)                                                          
   Lymph Node                                         (12)         (13)         (11)         (8)                                    
      Hyperplasia, Lymphoid                                         1 (8%)                                                          
      Bronchial, Hemorrhage                                         1 (8%)                                                          
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    4                                                              
                                                                                                                                   
NTP Experiment-Test: 96017-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                         WATER DISINFECTION BYPRODUCTS (DIBROMOACETIC ACID)                    Date: 05/19/04    
Route: DOSED WATER                                                                                                Time: 09:07:01    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/L       50 MG/L      500 MG/L     1000                                      
                                                                                          MG/L                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
HEMATOPOIETIC SYSTEM - CONT                                                                                                         
      Bronchial, Hyperplasia, Lymphoid                              1 (8%)                                                          
      Deep Cervical, Hemorrhage                                                               1 (13%)                               
      Iliac, Hematopoietic Cell Proliferation                       1 (8%)                                                          
      Iliac, Hemorrhage                                1 (8%)                                 1 (13%)                               
      Iliac, Hyperplasia, Lymphoid                     1 (8%)       1 (8%)       1 (9%)       2 (25%)                               
      Iliac, Pigmentation                              1 (8%)                                                                       
      Inguinal, Hematopoietic Cell Proliferation                    1 (8%)                                                          
      Inguinal, Hyperplasia, Lymphoid                               2 (15%)                                                         
      Mediastinal, Hemorrhage                          1 (8%)                                                                       
      Mediastinal, Hyperplasia, Lymphoid               1 (8%)       1 (8%)       1 (9%)       1 (13%)                               
      Mediastinal, Pigmentation                        1 (8%)                                                                       
      Pancreatic, Hyperplasia, Lymphoid                                          1 (9%)                                             
      Renal, Hyperplasia, Lymphoid                     1 (8%)                    1 (9%)       1 (13%)                               
   Lymph Node, Mandibular                             (48)         (49)         (47)         (50)                                   
      Atrophy                                                       1 (2%)       1 (2%)       1 (2%)                                
      Hematopoietic Cell Proliferation                 1 (2%)       1 (2%)                    2 (4%)                                
      Hemorrhage                                                    2 (4%)       2 (4%)       1 (2%)                                
      Hyperplasia, Lymphoid                           13 (27%)      6 (12%)      8 (17%)      9 (18%)                               
      Pigmentation                                    18 (38%)     25 (51%)     17 (36%)     23 (46%)                               
   Lymph Node, Mesenteric                             (49)         (49)         (48)         (45)                                   
      Angiectasis                                                                1 (2%)                                             
      Atrophy                                                                    2 (4%)       1 (2%)                                
      Ectasia                                                                    1 (2%)                                             
      Hematopoietic Cell Proliferation                 2 (4%)                    2 (4%)       1 (2%)                                
      Hemorrhage                                       1 (2%)       1 (2%)       1 (2%)       5 (11%)                               
      Hyperplasia, Lymphoid                            8 (16%)      9 (18%)      7 (15%)      5 (11%)                               
      Pigmentation                                                               1 (2%)       1 (2%)                                
   Spleen                                             (50)         (50)         (50)         (48)                                   
      Hematopoietic Cell Proliferation                34 (68%)     42 (84%)     35 (70%)     34 (71%)                               
      Necrosis                                         1 (2%)                                                                       
      Pigmentation                                     5 (10%)      5 (10%)      1 (2%)       2 (4%)                                
      Lymphoid Follicle, Atrophy                                    1 (2%)       2 (4%)       2 (4%)                                
      Lymphoid Follicle, Hyperplasia                   8 (16%)     11 (22%)     14 (28%)     12 (25%)                               
   Thymus                                             (44)         (47)         (47)         (48)                                   
      Atrophy                                          8 (18%)      9 (19%)     11 (23%)     13 (27%)                               
      Hemorrhage                                       1 (2%)                                                                       
      Hyperplasia, Lymphoid                                         3 (6%)       3 (6%)       2 (4%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (50)         (50)         (50)         (50)                                   
      Hyperplasia                                      5 (10%)      6 (12%)      2 (4%)       5 (10%)                               
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    5                                                              
                                                                                                                                   
NTP Experiment-Test: 96017-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                         WATER DISINFECTION BYPRODUCTS (DIBROMOACETIC ACID)                    Date: 05/19/04    
Route: DOSED WATER                                                                                                Time: 09:07:01    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/L       50 MG/L      500 MG/L     1000                                      
                                                                                          MG/L                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
INTEGUMENTARY SYSTEM - CONT                                                                                                         
   Skin                                               (50)         (50)         (50)         (50)                                   
      Edema                                                         1 (2%)       1 (2%)       1 (2%)                                
      Erosion                                                                    1 (2%)                                             
      Ulcer                                            2 (4%)       1 (2%)       1 (2%)       1 (2%)                                
      Epidermis, Hyperplasia                           2 (4%)       1 (2%)       2 (4%)       1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (50)         (50)         (50)         (50)                                   
      Myelofibrosis                                    1 (2%)       2 (4%)       1 (2%)       2 (4%)                                
   Skeletal Muscle                                    (3)          (4)          (2)          (5)                                    
      Inflammation, Chronic Active                                                            1 (20%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (50)         (49)         (50)         (50)                                   
      Compression                                      1 (2%)       1 (2%)       1 (2%)                                             
      Hemorrhage                                                    2 (4%)                                                          
      Necrosis                                                      1 (2%)       1 (2%)                                             
   Peripheral Nerve                                                (1)          (1)          (1)                                    
      Atrophy                                                                                 1 (100%)                              
   Spinal Cord                                                     (1)          (1)          (1)                                    
      Necrosis                                                      1 (100%)                                                        
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (50)         (50)         (50)         (50)                                   
      Congestion                                                                 2 (4%)                                             
      Hemorrhage                                                    2 (4%)       2 (4%)       3 (6%)                                
      Hyperplasia, Lymphoid                            5 (10%)      5 (10%)      1 (2%)       3 (6%)                                
      Infiltration Cellular, Histiocyte                1 (2%)       2 (4%)       2 (4%)       4 (8%)                                
      Alveolar Epithelium, Hyperplasia                 3 (6%)       3 (6%)                    4 (8%)                                
      Perivascular, Inflammation, Chronic Active                                              1 (2%)                                
   Nose                                               (50)         (50)         (50)         (50)                                   
      Inflammation, Chronic                                                                   1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Eye                                                (50)         (50)         (50)         (50)                                   
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    6                                                              
                                                                                                                                   
NTP Experiment-Test: 96017-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                         WATER DISINFECTION BYPRODUCTS (DIBROMOACETIC ACID)                    Date: 05/19/04    
Route: DOSED WATER                                                                                                Time: 09:07:01    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/L       50 MG/L      500 MG/L     1000                                      
                                                                                          MG/L                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
SPECIAL SENSES SYSTEM - CONT                                                                                                        
      Atrophy                                                                    1 (2%)                                             
      Cataract                                                                   2 (4%)       2 (4%)                                
      Inflammation, Chronic                            2 (4%)       2 (4%)       3 (6%)       3 (6%)                                
      Cornea, Hyperplasia                              1 (2%)                    4 (8%)                                             
      Cornea, Mineralization                                                                  1 (2%)                                
   Harderian Gland                                    (49)         (50)         (50)         (50)                                   
      Hyperplasia, Focal                               2 (4%)       4 (8%)       4 (8%)       2 (4%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (49)         (50)         (50)         (49)                                   
      Cyst                                                          1 (2%)                    1 (2%)                                
      Hydronephrosis                                                             1 (2%)       1 (2%)                                
      Hyperplasia, Lymphoid                            7 (14%)      2 (4%)       5 (10%)      8 (16%)                               
      Infarct                                          3 (6%)       8 (16%)      6 (12%)      8 (16%)                               
      Nephropathy                                     14 (29%)      8 (16%)     15 (30%)     12 (24%)                               
      Renal Tubule, Accumulation, Hyaline Droplet      1 (2%)       1 (2%)       2 (4%)       2 (4%)                                
      Renal Tubule, Dilatation                                      2 (4%)       1 (2%)       1 (2%)                                
      Renal Tubule, Necrosis                                        1 (2%)                    1 (2%)                                
      Renal Tubule, Pigmentation                       1 (2%)                    3 (6%)       1 (2%)                                
   Urinary Bladder                                    (50)         (49)         (50)         (49)                                   
      Edema                                            2 (4%)                                                                       
      Hyperplasia, Lymphoid                            3 (6%)       5 (10%)      3 (6%)       2 (4%)                                
      Transitional Epithelium, Hyperplasia                                       1 (2%)                                             
                                                                                                                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page    7                                                              
                                                                                                                                   
NTP Experiment-Test: 96017-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                         WATER DISINFECTION BYPRODUCTS (DIBROMOACETIC ACID)                    Date: 05/19/04    
Route: DOSED WATER                                                                                                Time: 09:07:01    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          0 MG/L       50 MG/L      500 MG/L     1000                                      
                                                                                          MG/L                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially In Study                          50           50           50           50                                     
  Early Deaths                                                                                                                      
    Moribund Sacrifice                                 4            6            3            6                                     
    Natural Death                                     14            6           13           13                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                31           38           34           31                                     
  Other                                                1                                                                            
                                                                                                                                    
  Animals Examined Microscopically                    49           50           50           50                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Intestine Large, Cecum                             (43)         (49)         (48)         (46)                                   
      Edema                                            2 (5%)       2 (4%)       3 (6%)       1 (2%)                                
      Hyperplasia, Lymphoid                                                      1 (2%)                                             
   Intestine Small, Duodenum                          (42)         (50)         (46)         (48)                                   
      Hyperplasia, Lymphoid                                         1 (2%)                                                          
   Intestine Small, Jejunum                           (44)         (49)         (46)         (45)                                   
      Hyperplasia, Lymphoid                                                      2 (4%)       2 (4%)                                
   Liver                                              (49)         (50)         (50)         (50)                                   
      Angiectasis                                                                1 (2%)                                             
      Basophilic Focus                                 2 (4%)                    1 (2%)       2 (4%)                                
      Clear Cell Focus                                10 (20%)     13 (26%)     23 (46%)     13 (26%)                               
      Congestion                                       1 (2%)                                                                       
      Cyst                                                          1 (2%)                    1 (2%)                                
      Eosinophilic Focus                              15 (31%)     10 (20%)     15 (30%)     17 (34%)                               
      Hematopoietic Cell Proliferation                                                        1 (2%)                                
      Hemorrhage                                       1 (2%)                                                                       
      Hepatodiaphragmatic Nodule                                    1 (2%)                                                          
      Infarct                                          1 (2%)                    1 (2%)                                             
      Infiltration Cellular, Mixed Cell                2 (4%)       4 (8%)       2 (4%)       2 (4%)                                
      Mixed Cell Focus                                 3 (6%)       7 (14%)      8 (16%)      4 (8%)                                
      Necrosis, Focal                                  8 (16%)      7 (14%)      3 (6%)       5 (10%)                               
      Tension Lipidosis                                             1 (2%)       1 (2%)       2 (4%)                                
      Bile Duct, Cyst                                                            1 (2%)                                             
      Bile Duct, Cyst Multilocular                                               1 (2%)                                             
      Centrilobular, Necrosis                          4 (8%)       4 (8%)                    5 (10%)                               
      Hepatocyte, Vacuolization Cytoplasmic            8 (16%)      9 (18%)      4 (8%)      10 (20%)                               
      Kupffer Cell, Pigmentation                       1 (2%)       1 (2%)       1 (2%)       1 (2%)                                
   Mesentery                                          (7)          (7)          (10)         (7)                                    
      Inflammation, Chronic                                                      1 (10%)                                            
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    8                                                              
                                                                                                                                   
NTP Experiment-Test: 96017-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                         WATER DISINFECTION BYPRODUCTS (DIBROMOACETIC ACID)                    Date: 05/19/04    
Route: DOSED WATER                                                                                                Time: 09:07:01    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          0 MG/L       50 MG/L      500 MG/L     1000                                      
                                                                                          MG/L                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - CONT                                                                                                            
      Fat, Necrosis                                    5 (71%)      6 (86%)      7 (70%)      4 (57%)                               
   Pancreas                                           (46)         (50)         (50)         (49)                                   
      Necrosis                                                      1 (2%)                                                          
      Acinus, Cytoplasmic Alteration                   2 (4%)       2 (4%)       2 (4%)                                             
   Salivary Glands                                    (49)         (50)         (50)         (50)                                   
      Atrophy                                          1 (2%)                                 2 (4%)                                
      Hyperplasia, Lymphoid                            3 (6%)       4 (8%)       6 (12%)      1 (2%)                                
   Stomach, Forestomach                               (49)         (50)         (50)         (50)                                   
      Diverticulum                                                  2 (4%)       1 (2%)                                             
      Erosion                                          1 (2%)                    1 (2%)       2 (4%)                                
      Inflammation, Chronic                            3 (6%)       1 (2%)       3 (6%)       6 (12%)                               
      Ulcer                                            2 (4%)       2 (4%)       2 (4%)       2 (4%)                                
      Epithelium, Hyperplasia                          4 (8%)       3 (6%)       4 (8%)       7 (14%)                               
      Epithelium, Hyperplasia, Focal                                             1 (2%)       1 (2%)                                
   Stomach, Glandular                                 (47)         (50)         (50)         (50)                                   
      Erosion                                          2 (4%)       2 (4%)       1 (2%)                                             
      Glands, Hyperplasia                                           1 (2%)                                                          
   Tooth                                              (5)          (6)                                                              
      Malformation                                     5 (100%)     6 (100%)                                                        
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (49)         (50)         (50)         (50)                                   
      Cardiomyopathy                                   1 (2%)       1 (2%)                                                          
      Inflammation, Chronic                            1 (2%)                                                                       
      Mineralization                                   3 (6%)                    2 (4%)       1 (2%)                                
      Myocardium, Necrosis                                          1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (49)         (50)         (50)         (50)                                   
      Accessory Adrenal Cortical Nodule                7 (14%)      5 (10%)      4 (8%)       6 (12%)                               
      Hyperplasia, Focal                               4 (8%)       7 (14%)      5 (10%)      1 (2%)                                
      Hypertrophy                                      1 (2%)                                 1 (2%)                                
      Hypertrophy, Focal                              12 (24%)     11 (22%)     13 (26%)      6 (12%)                               
      Capsule, Hyperplasia                             7 (14%)      4 (8%)       1 (2%)       5 (10%)                               
   Adrenal Medulla                                    (49)         (50)         (50)         (50)                                   
      Hyperplasia                                      1 (2%)       1 (2%)                    1 (2%)                                
   Islets, Pancreatic                                 (46)         (50)         (50)         (49)                                   
      Atrophy                                                                                 1 (2%)                                
      Hyperplasia                                     34 (74%)     32 (64%)     26 (52%)     13 (27%)                               
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    9                                                              
                                                                                                                                   
NTP Experiment-Test: 96017-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                         WATER DISINFECTION BYPRODUCTS (DIBROMOACETIC ACID)                    Date: 05/19/04    
Route: DOSED WATER                                                                                                Time: 09:07:01    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          0 MG/L       50 MG/L      500 MG/L     1000                                      
                                                                                          MG/L                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ENDOCRINE SYSTEM - CONT                                                                                                             
   Parathyroid Gland                                  (45)         (47)         (49)         (47)                                   
      Cyst                                                          2 (4%)       3 (6%)       4 (9%)                                
   Pituitary Gland                                    (45)         (50)         (48)         (48)                                   
      Pars Distalis, Cyst                              7 (16%)      7 (14%)      7 (15%)      5 (10%)                               
      Pars Distalis, Hyperplasia, Focal                1 (2%)                    1 (2%)                                             
   Thyroid Gland                                      (48)         (50)         (50)         (50)                                   
      Degeneration, Cystic                             5 (10%)      8 (16%)      3 (6%)       6 (12%)                               
      Follicular Cell, Hyperplasia                     3 (6%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Epididymis                                         (49)         (50)         (50)         (50)                                   
      Atypia Cellular                                  5 (10%)      4 (8%)       2 (4%)       6 (12%)                               
      Granuloma Sperm                                  1 (2%)       2 (4%)       2 (4%)       2 (4%)                                
      Spermatocele                                     1 (2%)                                 1 (2%)                                
   Preputial Gland                                    (49)         (50)         (50)         (50)                                   
      Cyst                                            25 (51%)     26 (52%)     33 (66%)     33 (66%)                               
      Inflammation, Chronic                           21 (43%)     30 (60%)     24 (48%)     28 (56%)                               
   Prostate                                           (48)         (50)         (50)         (49)                                   
      Inflammation, Chronic                                         4 (8%)                    1 (2%)                                
   Seminal Vesicle                                    (49)         (50)         (50)         (50)                                   
      Degeneration                                     3 (6%)       5 (10%)      1 (2%)                                             
      Inflammation, Chronic                            1 (2%)                                                                       
   Testes                                             (49)         (50)         (50)         (50)                                   
      Germinal Epithelium, Atrophy                     9 (18%)      4 (8%)       4 (8%)       8 (16%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (47)         (50)         (50)         (50)                                   
      Angiectasis                                                                             1 (2%)                                
      Depletion Cellular                               1 (2%)                                                                       
      Hyperplasia                                     22 (47%)     22 (44%)     28 (56%)     32 (64%)                               
   Lymph Node                                         (1)          (2)          (5)          (1)                                    
      Bronchial, Hyperplasia, Lymphoid                                           1 (20%)                                            
      Iliac, Ectasia                                                1 (50%)                                                         
      Inguinal, Hyperplasia, Lymphoid                                            1 (20%)                                            
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page   10                                                              
                                                                                                                                   
NTP Experiment-Test: 96017-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                         WATER DISINFECTION BYPRODUCTS (DIBROMOACETIC ACID)                    Date: 05/19/04    
Route: DOSED WATER                                                                                                Time: 09:07:01    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          0 MG/L       50 MG/L      500 MG/L     1000                                      
                                                                                          MG/L                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
HEMATOPOIETIC SYSTEM - CONT                                                                                                         
      Inguinal, Pigmentation                                        1 (50%)                                                         
      Mediastinal, Hyperplasia, Lymphoid               1 (100%)                  1 (20%)                                            
      Mediastinal, Pigmentation                                                               1 (100%)                              
      Pancreatic, Hyperplasia, Lymphoid                                          1 (20%)                                            
   Lymph Node, Mandibular                             (48)         (50)         (48)         (48)                                   
      Atrophy                                          5 (10%)      2 (4%)       3 (6%)       7 (15%)                               
      Hematopoietic Cell Proliferation                 1 (2%)                                                                       
      Hemorrhage                                       1 (2%)                    1 (2%)                                             
      Hyperplasia, Lymphoid                           15 (31%)     14 (28%)      6 (13%)      7 (15%)                               
      Pigmentation                                     7 (15%)      8 (16%)      1 (2%)       7 (15%)                               
   Lymph Node, Mesenteric                             (46)         (49)         (49)         (47)                                   
      Atrophy                                          4 (9%)       2 (4%)       1 (2%)       9 (19%)                               
      Hematopoietic Cell Proliferation                 1 (2%)       1 (2%)       3 (6%)       3 (6%)                                
      Hemorrhage                                       8 (17%)      5 (10%)      9 (18%)      7 (15%)                               
      Hyperplasia, Lymphoid                            2 (4%)       8 (16%)      3 (6%)       6 (13%)                               
      Hyperplasia, Plasma Cell                                                   1 (2%)                                             
   Spleen                                             (47)         (50)         (50)         (50)                                   
      Angiectasis                                                                             1 (2%)                                
      Hematopoietic Cell Proliferation                18 (38%)     20 (40%)     28 (56%)     38 (76%)                               
      Pigmentation                                                  1 (2%)       1 (2%)       1 (2%)                                
      Lymphoid Follicle, Atrophy                       4 (9%)       7 (14%)      3 (6%)       8 (16%)                               
      Lymphoid Follicle, Hyperplasia                   3 (6%)       6 (12%)      6 (12%)      6 (12%)                               
   Thymus                                             (42)         (45)         (46)         (47)                                   
      Atrophy                                         14 (33%)     16 (36%)     12 (26%)     22 (47%)                               
      Hyperplasia, Lymphoid                                         1 (2%)       1 (2%)       1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Skin                                               (49)         (50)         (50)         (50)                                   
      Cyst Epithelial Inclusion                                     2 (4%)                                                          
      Ulcer                                            3 (6%)       2 (4%)                    3 (6%)                                
      Epidermis, Hyperplasia                           3 (6%)       2 (4%)                    3 (6%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (49)         (50)         (50)         (50)                                   
      Hyperostosis                                                  1 (2%)                                                          
      Cranium, Hyperostosis                            1 (2%)                                 1 (2%)                                
   Skeletal Muscle                                    (1)          (1)                       (1)                                    
      Atrophy                                                       1 (100%)                                                        
____________________________________________________________________________________________________________________________________
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page   11                                                              
                                                                                                                                   
NTP Experiment-Test: 96017-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                         WATER DISINFECTION BYPRODUCTS (DIBROMOACETIC ACID)                    Date: 05/19/04    
Route: DOSED WATER                                                                                                Time: 09:07:01    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          0 MG/L       50 MG/L      500 MG/L     1000                                      
                                                                                          MG/L                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Peripheral Nerve                                   (2)          (2)          (1)                                                 
      Atrophy                                                       1 (50%)      1 (100%)                                           
   Spinal Cord                                        (2)          (2)          (1)                                                 
      Gliosis                                          1 (50%)                                                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (49)         (50)         (50)         (50)                                   
      Edema                                            2 (4%)                    1 (2%)       2 (4%)                                
      Hemorrhage                                       2 (4%)                                 4 (8%)                                
      Hyperplasia, Lymphoid                            3 (6%)                    1 (2%)                                             
      Infiltration Cellular, Histiocyte                2 (4%)       6 (12%)      3 (6%)       6 (12%)                               
      Metaplasia, Osseous                              1 (2%)       2 (4%)                    1 (2%)                                
      Alveolar Epithelium, Hyperplasia                 2 (4%)       6 (12%)      6 (12%)      7 (14%)                               
      Bronchiole, Hyperplasia                                                                 1 (2%)                                
   Nose                                               (48)         (50)         (50)         (50)                                   
      Foreign Body                                                               1 (2%)                                             
      Inflammation, Chronic                            1 (2%)       1 (2%)       3 (6%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Eye                                                (49)         (50)         (50)         (50)                                   
      Atrophy                                                       1 (2%)                                                          
      Cataract                                                      1 (2%)       1 (2%)       5 (10%)                               
      Inflammation, Chronic                                                      2 (4%)       2 (4%)                                
      Bilateral, Cataract                                                                     1 (2%)                                
      Cornea, Hyperplasia                                                        1 (2%)       1 (2%)                                
   Harderian Gland                                    (49)         (50)         (50)         (50)                                   
      Cyst                                                          1 (2%)                                                          
      Hyperplasia, Focal                               5 (10%)      1 (2%)       1 (2%)       1 (2%)                                
   Lacrimal Gland                                                  (1)                                                              
      Cyst                                                          1 (100%)                                                        
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (49)         (50)         (50)         (50)                                   
      Cyst                                             9 (18%)     13 (26%)     14 (28%)     11 (22%)                               
      Hydronephrosis                                   4 (8%)       2 (4%)                    4 (8%)                                
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page   12                                                              
                                                                                                                                   
NTP Experiment-Test: 96017-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                         WATER DISINFECTION BYPRODUCTS (DIBROMOACETIC ACID)                    Date: 05/19/04    
Route: DOSED WATER                                                                                                Time: 09:07:01    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          0 MG/L       50 MG/L      500 MG/L     1000                                      
                                                                                          MG/L                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
URINARY SYSTEM - CONT                                                                                                               
      Hyperplasia, Lymphoid                            5 (10%)      5 (10%)      6 (12%)      5 (10%)                               
      Infarct                                          3 (6%)       7 (14%)      4 (8%)       5 (10%)                               
      Inflammation, Suppurative                                     1 (2%)                    1 (2%)                                
      Metaplasia, Osseous                              6 (12%)      3 (6%)       2 (4%)       2 (4%)                                
      Nephropathy                                     36 (73%)     44 (88%)     42 (84%)     39 (78%)                               
      Papilla, Necrosis                                                                       1 (2%)                                
      Renal Tubule, Accumulation, Hyaline Droplet                                1 (2%)                                             
      Renal Tubule, Dilatation                         1 (2%)       3 (6%)                    1 (2%)                                
      Renal Tubule, Hyperplasia                        1 (2%)       1 (2%)       1 (2%)       3 (6%)                                
      Renal Tubule, Necrosis                           1 (2%)                                                                       
      Renal Tubule, Pigmentation                       1 (2%)       3 (6%)       2 (4%)       7 (14%)                               
      Transitional Epithelium, Hyperplasia                                       1 (2%)                                             
   Urethra                                                         (3)                                                              
      Angiectasis                                                   3 (100%)                                                        
      Inflammation, Chronic Active                                  3 (100%)                                                        
   Urinary Bladder                                    (49)         (50)         (50)         (49)                                   
      Edema                                                                      1 (2%)                                             
      Hemorrhage                                                    1 (2%)                                                          
      Hyperplasia, Lymphoid                                         1 (2%)       1 (2%)                                             
      Inflammation, Chronic                                                                   1 (2%)                                
      Transitional Epithelium, Hyperplasia                                       1 (2%)       1 (2%)                                
                                                                                                                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   13                                                              
                                                                                                                                   
                             ------------------------------------------------------------                                           
                             ----------              END OF REPORT             ----------                                           
                             ------------------------------------------------------------                                           
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.