Test Method Evaluations


Acute Systemic Toxicity

Acute systemic toxicity tests assess the likelihood of chemical or medical products to cause illness following a single exposure. Read about evaluations of approaches that reduce animal use for these tests. Go


Biologics are medical products derived from biological sources. Learn more about methods that use fewer or no animals to evaluate the safety of biologics. Go

In Vitro Cardiotoxicity Screening Approaches

Cardiotoxicity is a major cause of failure of new drugs. NICEATM is supporting an initiative to design non-animal approaches to assess cardiotoxicity hazard. Go

Developmental Toxicity

Developmental toxicity tests assess how substances interfere with normal development and impact offspring. Learn more about alternatives to traditional animal studies for developmental toxicity. Go

Endocrine Disruptors

Endocrine disruptors are substances that mimic or inhibit the function of hormones. Read about non-animal approaches to identify endocrine disruptors. Go

Microphysiological Systems

NICEATM evaluates the use of microphysiological systems, or tissue chips, to replace, reduce, or refine animal use in testing. Go

Ocular & Dermal Toxicity

Ocular and dermal toxicity tests examine how substances interact with eyes and skin. Learn more about evaluations of non-animal testing approaches. Go


Pyrogens are substances that can produce a fever. Learn more about ICCVAM evaluation of test methods proposed as replacements for the rabbit pyrogen test. Go

Skin Sensitization

Skin sensitizers can cause allergic skin rashes. Read about how NICEATM and ICCVAM have developed approaches to identify these substances without using animals. Go