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Acute Systemic Toxicity Testing

Acute systemic toxicity tests are used to assess the toxicity of chemicals, medical products, and other substances resulting from different types of exposure. These tests are used to determine if substances need protective packaging, warning labels, and environmental release guides, or if protective equipment may be required for handling. Learn about alternatives to animal use for these tests.


Rainbow trout

Fish Acute Toxicity

NICEATM is working with EPA and other partners to explore reducing animal use for fish acute toxicity testing Go
Diagram of the U.S. Strategic Roadmap

Predictive Models

ICCVAM invited scientists to develop computer models to predict acute oral systemic toxicity. Read more about these models. Go
Circular diagram of utilization, confidence, and technology

Replacing Animals

The U.S. strategy and roadmap is guiding replacement of animal use for required acute systemic toxicity testing. Find out about related NICEATM and ICCVAM activities. Go
Scientist in safety gear to prevent inhalation of chemicals

Acute Inhalation Toxicity

NICEATM and collaborators have held workshops to consider how non-animal methods could be used to identify substances that are toxic when inhaled. Read more about the workshops. Go
Cultured cells under microscope

Validation Study of In Vitro Cytotoxicity Test Methods

NICEATM coordinated a study of how in vitro cytotoxicity test methods could reduce animal use for acute toxicity testing. Read more about the study. Go
Male scientist reviewing data in lab

Evaluation of In Vitro Cytotoxicity Test Methods

ICCVAM evaluated the use of cytotoxicity test methods for acute toxicity testing. Read more about the evaluation. Go
Fireman pouring absorbent on oil spilled from a wreck

Oral Up-and-Down Procedure (UDP)

The UDP is an alternative to the oral LD50 test that reduces animal use. Find out more about the ICCVAM evaluation of the UDP. Go