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OptiSafe Test Method

OptiSafe is an in vitro test method in which a test substance is applied to a semipermeable membrane. Damage to macromolecules in the membrane is measured to assess the test substance’s potential to cause eye irritation.

NICEATM coordinated a validation study of the OptiSafe test method to demonstrate the accuracy and reliability of the method among the test method developer’s laboratory and two naïve laboratories. Members of the ICCVAM Ocular and Dermal Irritation Workgroup comprise the validation management team for the study, and the study received support from an NIEHS Small Business Innovation Research grant. Testing was completed in late 2017. A summary of study results was presented (Choksi et al.) at the 2018 Society of Toxicology meeting; a study report has been submitted for publication.