INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)
ETHYLENE GLYCOL
NTP Experiment-Test: 05025-01 Report: PEIRPT03
Study Type: CHRONIC Date: 09/04/94
Route: DOSED FEED Time: 06:04:38
Facility: Southern Research Institute
Chemical CAS #: 107-21-1
Lock Date: None
Cage Range: All
Reasons For Removal: All
Removal Date Range: All
Treatment Groups: Include All
a Number of animals examined microscopically at site and number of animals with lesion
Page 1
NTP Experiment-Test: 05025-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ETHYLENE GLYCOL Date: 09/04/94
Route: DOSED FEED Time: 06:04:38
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE UNTREATD 1.25% 2.5% 5.0%
CONTROL
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 60 60 60 60
Scheduled Sacrifice 10 10 9 10
Early Deaths
Moribund 9 9 12 12
Dead 8 11 9 1
Survivors
Terminal Sacrifice 33 30 30 37
Animals Examined Microscopically 50 50 51 50
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Gallbladder (48) (18) (21) (49)
Inflammation 1 (2%)
Intestine Large, Cecum (48) (19) (16) (50)
Edema 1 (2%)
Lymphoid Tissue, Hyperplasia, Lymphoid 1 (2%)
Intestine Large, Colon (49) (19) (19) (50)
Inflammation, Suppurative 1 (2%)
Intestine Large, Rectum (49) (19) (17) (50)
Edema 1 (2%)
Intestine Small, Ileum (47) (19) (16) (50)
Amyloid Deposition 2 (4%)
Dilatation 1 (6%)
Inflammation, Chronic 1 (6%)
Lymphoid Tissue, Hyperplasia, Lymphoid 2 (4%)
Lymphoid Tissue, Inflammation, Granulomatous 1 (2%)
Intestine Small, Jejunum (47) (19) (16) (50)
Amyloid Deposition 1 (5%) 1 (2%)
Dilatation 1 (6%)
Liver (50) (50) (51) (50)
Angiectasis 1 (2%)
Basophilic Focus 1 (2%) 1 (2%)
Congestion 1 (2%) 1 (2%)
Cyst 1 (2%)
Fatty Change 2 (4%) 3 (6%)
Fibrosis 1 (2%)
Hematopoietic Cell Proliferation 2 (4%) 6 (12%) 7 (14%) 2 (4%)
Hyperplasia, Lymphoid 14 (28%) 14 (28%) 16 (31%) 12 (24%)
Infarct 1 (2%) 1 (2%) 1 (2%) 1 (2%)
Infiltration Cellular, Mixed Cell 4 (8%) 10 (20%) 11 (22%) 4 (8%)
Inflammation, Multifocal 17 (34%) 10 (20%) 19 (37%) 24 (48%)
Mineralization 1 (2%)
Necrosis, Multifocal 2 (4%) 2 (4%) 1 (2%) 2 (4%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 2
NTP Experiment-Test: 05025-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ETHYLENE GLYCOL Date: 09/04/94
Route: DOSED FEED Time: 06:04:38
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE UNTREATD 1.25% 2.5% 5.0%
CONTROL
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Pigmentation 1 (2%)
Bile Duct, Hyperplasia 1 (2%)
Hepatocyte, Erythrophagocytosis 2 (4%)
Hepatocyte, Centrilobular, Degeneration,
Hyaline 1 (2%) 26 (52%)
Portal Vein, Media, Hyperplasia 1 (2%)
Mesentery (17) (7) (15) (7)
Cyst 1 (7%)
Edema 1 (14%)
Hemorrhage 1 (6%)
Inflammation, Suppurative 6 (35%) 5 (71%) 9 (60%) 2 (29%)
Fat, Necrosis 8 (47%) 1 (14%)
Pancreas (49) (21) (22) (50)
Atrophy, Diffuse 2 (10%)
Atrophy, Focal 1 (5%) 3 (6%)
Ectopic Tissue 1 (2%)
Hyperplasia, Lymphoid 2 (4%)
Inflammation, Suppurative 1 (5%) 1 (5%)
Acinar Cell, Hypoplasia, Focal 1 (2%)
Duct, Dilatation 1 (2%) 1 (5%) 1 (5%) 3 (6%)
Duct, Dilatation, Multiple 1 (2%)
Salivary Glands (49) (20) (21) (50)
Hyperplasia, Focal 1 (2%)
Stomach, Forestomach (50) (20) (20) (50)
Inflammation, Focal 1 (5%) 2 (4%)
Mineralization 1 (2%) 1 (2%)
Mucosa, Hyperplasia, Papillary 1 (2%)
Stomach, Glandular (49) (20) (20) (50)
Hyperplasia, Lymphoid 2 (4%) 1 (2%)
Inflammation, Focal 1 (2%) 4 (8%)
Mineralization 1 (2%) 3 (6%)
Epithelium, Degeneration, Hyaline 2 (4%)
Epithelium, Metaplasia, Squamous 1 (2%)
Mucosa, Cyst 2 (4%)
Tooth (2) (4) (1) (3)
Incisor, Dysplasia 2 (100%) 4 (100%) 1 (100%) 3 (100%)
Incisor, Inflammation, Suppurative 1 (25%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Blood Vessel (2) (1)
Aorta, Thrombosis 1 (50%)
Mesenteric Artery, Inflammation, Chronic 1 (50%) 1 (100%)
Heart (50) (20) (21) (50)
Infiltration Cellular, Mixed Cell 1 (5%)
Inflammation, Suppurative 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 3
NTP Experiment-Test: 05025-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ETHYLENE GLYCOL Date: 09/04/94
Route: DOSED FEED Time: 06:04:38
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE UNTREATD 1.25% 2.5% 5.0%
CONTROL
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM - CONT
Artery, Media, Hyperplasia 1 (2%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Gland, Cortex (49) (21) (21) (50)
Accessory Adrenal Cortical Nodule 1 (2%)
Amyloid Deposition 1 (2%)
Angiectasis 1 (2%)
Focal Cellular Change 1 (5%)
Hematopoietic Cell Proliferation 1 (5%)
Hemorrhage, Focal 1 (2%)
Hyperplasia, Focal 1 (2%)
Infiltration Cellular, Mixed Cell 1 (5%)
Inflammation, Suppurative 1 (2%)
Vacuolization Cytoplasmic, Focal 2 (4%)
Subcapsular, Hyperplasia, Focal 1 (2%)
Adrenal Gland, Medulla (48) (21) (21) (50)
Ectopic Tissue 2 (4%)
Hyperplasia, Focal 1 (2%) 1 (5%) 1 (2%)
Parathyroid Gland (49) (18) (14) (42)
Cyst 1 (2%)
Pituitary Gland (47) (22) (23) (47)
Pars Distalis, Angiectasis 1 (2%)
Pars Distalis, Bacterium 1 (2%)
Pars Distalis, Cyst 2 (4%)
Pars Distalis, Focal Cellular Change 2 (9%) 2 (9%)
Pars Distalis, Hyperplasia, Focal 13 (28%) 2 (9%) 14 (30%)
Pars Distalis, Necrosis, Focal 1 (5%)
Pars Nervosa, Inflammation, Focal 1 (4%)
Thyroid Gland (49) (50) (51) (50)
Degeneration, Cystic 4 (8%) 6 (12%) 4 (8%) 4 (8%)
Hyperplasia, Lymphoid 1 (2%)
Inflammation, Focal 3 (6%) 3 (6%) 3 (6%)
C-Cell, Hyperplasia 1 (2%) 1 (2%)
Follicular Cell, Hyperplasia 8 (16%) 16 (32%) 22 (43%) 14 (28%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
Tissue NOS (2) (1)
Abdominal, Foreign Body 1 (50%)
Abdominal, Infiltration Cellular, Lymphocyte 1 (100%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 4
NTP Experiment-Test: 05025-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ETHYLENE GLYCOL Date: 09/04/94
Route: DOSED FEED Time: 06:04:38
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE UNTREATD 1.25% 2.5% 5.0%
CONTROL
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Ovary (49) (25) (25) (48)
Angiectasis 1 (2%) 2 (8%) 3 (6%)
Atrophy 2 (4%)
Fibrosis, Focal 1 (2%)
Granuloma 1 (4%)
Hemorrhage 1 (4%) 2 (4%)
Hyperplasia, Lymphoid 1 (4%)
Inflammation, Chronic 1 (4%)
Inflammation, Suppurative 8 (16%) 10 (40%) 9 (36%) 3 (6%)
Mineralization 2 (4%)
Thrombosis 1 (4%)
Follicle, Cyst 11 (22%) 5 (20%) 8 (32%) 9 (19%)
Follicle, Cyst, Multiple 1 (4%)
Oviduct (3) (4) (1)
Hyperplasia, Cystic 1 (100%)
Inflammation, Suppurative 2 (67%) 4 (100%)
Uterus (50) (45) (45) (50)
Angiectasis 4 (9%) 1 (2%) 2 (4%)
Hemorrhage 1 (2%) 1 (2%) 2 (4%)
Hydrometra 3 (6%) 4 (9%) 2 (4%) 4 (8%)
Inflammation, Suppurative 6 (12%) 7 (16%) 5 (11%) 3 (6%)
Thrombosis 1 (2%)
Endometrium, Cyst 1 (2%) 2 (4%)
Endometrium, Hyperplasia, Cystic 44 (88%) 40 (89%) 40 (89%) 47 (94%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (50) (20) (21) (50)
Hyperplasia, Neutrophil 1 (2%) 1 (5%) 1 (5%) 2 (4%)
Lymph Node (50) (24) (22) (50)
Iliac, Hyperplasia 1 (4%) 4 (18%) 1 (2%)
Inguinal, Hyperplasia 1 (2%)
Inguinal, Hyperplasia, Lymphoid 1 (5%)
Mediastinal, Angiectasis 1 (4%)
Mediastinal, Hyperplasia 3 (6%) 3 (13%) 3 (14%) 1 (2%)
Mediastinal, Hyperplasia, Lymphoid 1 (2%) 2 (8%) 2 (4%)
Mediastinal, Inflammation, Suppurative 5 (10%) 1 (4%) 1 (5%)
Mesenteric, Angiectasis 4 (8%) 2 (8%) 6 (12%)
Mesenteric, Hyperplasia 2 (4%) 1 (4%) 2 (9%) 2 (4%)
Mesenteric, Hyperplasia, Lymphoid 5 (10%) 2 (9%) 7 (14%)
Mesenteric, Inflammation, Suppurative 2 (4%)
Pancreatic, Hyperplasia 1 (5%)
Renal, Hyperplasia 2 (4%) 1 (4%) 5 (23%) 1 (2%)
Renal, Inflammation, Suppurative 1 (2%) 1 (4%)
Lymph Node, Mandibular (43) (21) (20) (49)
a Number of animals examined microscopically at site and number of animals with lesion
Page 5
NTP Experiment-Test: 05025-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ETHYLENE GLYCOL Date: 09/04/94
Route: DOSED FEED Time: 06:04:38
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE UNTREATD 1.25% 2.5% 5.0%
CONTROL
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM - CONT
Hyperplasia 1 (2%) 2 (4%)
Hyperplasia, Lymphoid 2 (5%) 1 (5%) 1 (5%) 6 (12%)
Spleen (49) (29) (28) (50)
Depletion Cellular 1 (2%)
Fibrosis, Focal 1 (2%)
Hematopoietic Cell Proliferation 14 (29%) 12 (41%) 20 (71%) 13 (26%)
Hyperplasia, Lymphoid 11 (22%) 2 (7%) 6 (21%) 13 (26%)
Thymus (47) (19) (19) (47)
Cyst 1 (5%) 1 (2%)
Hyperplasia 1 (2%)
Hyperplasia, Lymphoid 7 (15%) 1 (5%) 6 (13%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Mammary Gland (49) (20) (21) (50)
Dilatation 4 (8%) 1 (5%) 6 (12%)
Hyperplasia 2 (4%)
Inflammation, Suppurative 1 (2%)
Duct, Metaplasia, Squamous 1 (2%)
Skin (49) (43) (40) (50)
Inflammation, Chronic 16 (33%) 5 (12%) 6 (15%) 11 (22%)
Subcutaneous Tissue, Edema 1 (2%)
Subcutaneous Tissue, Hyperplasia, Lymphoid 1 (2%)
Subcutaneous Tissue, Inflammation, Chronic 2 (4%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (50) (20) (21) (50)
Cranium, Hyperostosis 1 (2%)
Distal, Joint, Femur, Arthrosis 1 (2%)
Femur, Hyperostosis 1 (5%)
Skeletal Muscle (2) (2) (2) (1)
Inflammation, Suppurative 1 (50%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (49) (20) (21) (50)
Compression 1 (2%) 1 (5%) 1 (5%) 3 (6%)
Gliosis 1 (5%)
Hemorrhage, Multifocal 2 (4%)
Infiltration Cellular, Lymphocyte 1 (5%)
Necrosis, Focal 1 (5%)
Artery, Inflammation, Chronic 1 (5%)
Artery, Meninges, Inflammation, Chronic 1 (5%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 6
NTP Experiment-Test: 05025-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ETHYLENE GLYCOL Date: 09/04/94
Route: DOSED FEED Time: 06:04:38
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE UNTREATD 1.25% 2.5% 5.0%
CONTROL
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM - CONT
Meninges, Hyperplasia, Lymphoid 1 (2%)
Meninges, Infiltration Cellular, Mixed Cell 1 (5%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (50) (50) (51) (50)
Bacterium 1 (2%)
Congestion 2 (4%) 2 (4%)
Hemorrhage 2 (4%) 4 (8%)
Hyperplasia, Lymphoid 17 (34%) 16 (32%) 15 (29%) 21 (42%)
Hyperplasia, Macrophage 2 (4%) 2 (4%) 1 (2%)
Infiltration Cellular, Mixed Cell 3 (6%) 3 (6%) 3 (6%) 1 (2%)
Inflammation, Granulomatous 2 (4%)
Inflammation, Suppurative 1 (2%) 1 (2%)
Mineralization 1 (2%)
Pigmentation 1 (2%)
Alveolar Epithelium, Hyperplasia 1 (2%)
Artery, Inflammation 1 (2%)
Artery, Media, Hyperplasia 3 (6%) 10 (20%) 10 (20%) 23 (46%)
Bronchiole, Bronchiectasis 1 (2%)
Bronchiole, Epithelium, Hyperplasia 1 (2%)
Mediastinum, Inflammation, Suppurative 1 (2%) 3 (6%)
Vein, Intima, Mineralization 1 (2%)
Nose (50) (20) (21) (50)
Lumen, Exudate 2 (4%) 1 (2%)
Lumen, Foreign Body 1 (2%) 1 (2%)
Mucosa, Angiectasis 1 (2%)
Mucosa, Hemorrhage 1 (2%)
Nasolacrimal Duct, Inflammation, Suppurative 1 (2%)
Trachea (49) (20) (21) (50)
Artery, Peritracheal Tissue, Inflammation,
Chronic 1 (5%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Eye (1) (1)
Atrophy 1 (100%)
Cataract 1 (100%)
Fibrosis 1 (100%)
Retinal Detachment 1 (100%)
Cornea, Inflammation 1 (100%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 7
NTP Experiment-Test: 05025-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ETHYLENE GLYCOL Date: 09/04/94
Route: DOSED FEED Time: 06:04:38
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE UNTREATD 1.25% 2.5% 5.0%
CONTROL
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (49) (50) (50) (50)
Bacterium 1 (2%) 1 (2%)
Cyst 1 (2%)
Infiltration Cellular, Mixed Cell 1 (2%) 2 (4%) 1 (2%)
Inflammation, Chronic 1 (2%)
Inflammation, Suppurative 2 (4%) 2 (4%)
Metaplasia, Osseous 1 (2%) 1 (2%) 3 (6%)
Necrosis, Multifocal 1 (2%)
Nephropathy 25 (51%) 20 (40%) 33 (66%) 28 (56%)
Capsule, Inflammation, Suppurative 1 (2%)
Pelvis, Dilatation 1 (2%)
Renal Tubule, Degeneration, Hyaline 3 (6%) 2 (4%)
Renal Tubule, Dilatation 1 (2%) 1 (2%)
Renal Tubule, Hyperplasia 1 (2%)
Renal Tubule, Mineralization 1 (2%)
Renal Tubule, Pigmentation 2 (4%)
Renal Tubule, Vacuolization Cytoplasmic 1 (2%)
Urinary Bladder (49) (19) (21) (50)
Hyperplasia, Lymphoid 2 (4%) 5 (10%)
Inflammation, Chronic 1 (5%) 1 (2%)
Ulcer 1 (2%)
Transitional Epithelium, Hyperplasia 1 (5%)
Transitional Epithelium, Metaplasia, Squamous 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 8
NTP Experiment-Test: 05025-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ETHYLENE GLYCOL Date: 09/04/94
Route: DOSED FEED Time: 06:04:38
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE UNTREATD 0.625% 1.25% 2.5%
CONTROL
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 60 60 60 60
Scheduled Sacrifice 6 6 6 6
Early Deaths
Dead 15 8 11 16
Moribund 10 14 11 15
Survivors
Terminal Sacrifice 29 32 32 23
Animals Examined Microscopically 54 54 54 54
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Esophagus (54) (21) (22) (54)
Inflammation, Chronic 1 (2%)
Gallbladder (51) (19) (16) (48)
Inflammation 1 (2%) 1 (6%)
Mucosa, Hyperplasia 1 (6%)
Intestine Large, Cecum (52) (18) (18) (49)
Parasite Metazoan 1 (2%)
Intestine Large, Rectum (54) (19) (21) (48)
Inflammation, Chronic 1 (2%)
Prolapse 1 (5%)
Intestine Small, Duodenum (53) (20) (18) (46)
Mucosa, Inflammation, Suppurative 1 (2%)
Intestine Small, Ileum (53) (17) (17) (45)
Perforation 1 (6%)
Lymphoid Tissue, Hyperplasia, Lymphoid 1 (2%)
Intestine Small, Jejunum (54) (18) (19) (50)
Hyperplasia, Lymphoid 1 (2%)
Inflammation, Suppurative 1 (2%)
Liver (54) (53) (53) (54)
Angiectasis 1 (2%)
Autolysis 1 (2%)
Bacterium 2 (4%)
Basophilic Focus 1 (2%) 2 (4%) 2 (4%)
Congestion 1 (2%) 1 (2%)
Cyst 1 (2%)
Eosinophilic Focus 2 (4%) 1 (2%)
Fatty Change 2 (4%)
Fibrosis 2 (4%)
Focal Cellular Change 1 (2%) 1 (2%)
Hematopoietic Cell Proliferation 3 (6%) 1 (2%) 1 (2%)
Hyperplasia, Lymphoid 2 (4%) 3 (6%) 3 (6%) 2 (4%)
Hyperplasia, Multifocal 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 9
NTP Experiment-Test: 05025-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ETHYLENE GLYCOL Date: 09/04/94
Route: DOSED FEED Time: 06:04:38
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE UNTREATD 0.625% 1.25% 2.5%
CONTROL
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Infarct 1 (2%)
Infiltration Cellular, Mixed Cell 4 (7%) 5 (9%) 2 (4%) 8 (15%)
Inflammation, Multifocal 6 (11%) 11 (21%) 16 (30%) 13 (24%)
Necrosis, Multifocal 10 (19%) 6 (11%) 4 (8%) 8 (15%)
Pigmentation 1 (2%) 1 (2%) 3 (6%)
Artery, Media, Hypertrophy 1 (2%)
Bile Duct, Cyst 1 (2%)
Bile Duct, Hyperplasia 1 (2%)
Centrilobular, Necrosis 1 (2%) 2 (4%) 1 (2%)
Centrilobular, Vacuolization Nuclear 1 (2%)
Hepatocyte, Cytomegaly 1 (2%)
Hepatocyte, Centrilobular, Degeneration,
Hyaline 24 (45%) 36 (67%)
Mesentery (5) (3) (1) (7)
Inflammation, Chronic 2 (67%) 1 (100%) 1 (14%)
Inflammation, Granulomatous 1 (14%)
Inflammation, Suppurative 1 (20%) 3 (43%)
Fat, Necrosis 1 (14%)
Pancreas (54) (23) (21) (54)
Atrophy, Focal 1 (4%)
Fibrosis, Focal 1 (2%)
Focal Cellular Change 1 (2%)
Inflammation, Chronic, Focal 2 (4%)
Necrosis, Focal 1 (2%)
Duct, Degeneration, Hyaline 1 (2%)
Duct, Dilatation 1 (2%) 1 (5%)
Duct, Hyperplasia, Papillary 1 (2%)
Duct, Metaplasia 1 (2%)
Vein, Infiltration Cellular, Mixed Cell 1 (2%)
Salivary Glands (53) (21) (20) (54)
Atrophy, Focal 1 (2%)
Fibrosis, Focal 1 (2%)
Stomach, Forestomach (54) (20) (21) (54)
Mineralization 1 (2%)
Mucosa, Hyperplasia, Papillary 2 (4%)
Mucosa, Ulcer 1 (2%)
Stomach, Glandular (54) (20) (21) (54)
Erosion 1 (5%)
Inflammation, Focal 1 (2%) 1 (5%) 1 (2%)
Mineralization 2 (4%) 2 (10%)
Epithelium, Metaplasia, Squamous 1 (2%)
Epithelium, Vacuolization Cytoplasmic,
Multifocal 1 (2%)
Mucosa, Cyst 2 (4%)
Mucosa, Erosion 1 (2%)
Tooth (21) (4) (1) (18)
Incisor, Dysplasia 18 (86%) 4 (100%) 1 (100%) 15 (83%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 10
NTP Experiment-Test: 05025-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ETHYLENE GLYCOL Date: 09/04/94
Route: DOSED FEED Time: 06:04:38
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE UNTREATD 0.625% 1.25% 2.5%
CONTROL
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Incisor, Inflammation, Chronic 1 (5%)
Incisor, Inflammation, Suppurative 2 (10%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (54) (21) (22) (54)
Inflammation, Chronic 3 (6%) 1 (2%)
Inflammation, Focal 1 (2%)
Inflammation, Suppurative 1 (2%) 3 (6%)
Atrium, Thrombosis, Multiple 1 (2%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Gland, Cortex (54) (20) (21) (54)
Cyst 1 (2%)
Focal Cellular Change 1 (2%)
Hyperplasia, Focal 1 (5%) 1 (2%)
Infiltration Cellular, Mixed Cell 1 (2%)
Vacuolization Cytoplasmic, Focal 1 (2%)
Extra Adrenal Tissue, Fibrosis 1 (2%)
Extra Adrenal Tissue, Inflammation,
Suppurative 1 (2%)
Subcapsular, Hyperplasia, Focal 2 (4%) 2 (10%) 4 (7%)
Unilateral, Atrophy 1 (5%)
Adrenal Gland, Medulla (54) (21) (21) (53)
Hyperplasia, Focal 1 (2%) 1 (5%) 1 (2%)
Infiltration Cellular, Mixed Cell 1 (2%)
Parathyroid Gland (53) (18) (22) (50)
Cyst 1 (5%)
Pituitary Gland (52) (21) (17) (49)
Pars Distalis, Angiectasis 1 (6%)
Pars Distalis, Cyst 1 (2%)
Pars Distalis, Hyperplasia, Focal 5 (10%) 1 (6%) 2 (4%)
Thyroid Gland (53) (53) (54) (53)
Cyst 1 (2%) 2 (4%)
Degeneration, Cystic 12 (23%) 13 (25%) 13 (24%) 9 (17%)
Fibrosis 1 (2%)
Inflammation, Focal 1 (2%)
Follicle, Cyst 1 (2%) 4 (7%)
Follicular Cell, Hyperplasia 5 (9%) 8 (15%) 4 (7%) 9 (17%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 11
NTP Experiment-Test: 05025-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ETHYLENE GLYCOL Date: 09/04/94
Route: DOSED FEED Time: 06:04:38
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE UNTREATD 0.625% 1.25% 2.5%
CONTROL
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Coagulating Gland (1) (1)
Inflammation, Suppurative 1 (100%)
Adventitia, Hemorrhage 1 (100%)
Ductus Deferens (1)
Inflammation, Chronic 1 (100%)
Epididymis (54) (21) (22) (54)
Granuloma Sperm 1 (5%) 1 (2%)
Hyperplasia, Lymphoid 1 (2%)
Inflammation, Chronic 2 (4%)
Inflammation, Suppurative 1 (2%)
Spermatocele 1 (2%)
Penis (5) (1) (4) (6)
Fibrosis 1 (100%)
Hyperplasia 1 (17%)
Inflammation, Chronic 1 (20%) 1 (100%) 3 (75%) 1 (17%)
Inflammation, Suppurative 1 (20%)
Necrosis 2 (40%) 1 (25%)
Preputial Gland (12) (6) (7) (12)
Degeneration, Cystic 8 (67%) 3 (50%) 3 (43%) 6 (50%)
Inflammation, Suppurative 8 (67%) 5 (83%) 2 (29%) 7 (58%)
Prostate (53) (20) (22) (54)
Dilatation 1 (2%)
Edema 1 (2%)
Inflammation, Chronic 6 (11%) 1 (5%) 5 (23%) 10 (19%)
Seminal Vesicle (52) (23) (23) (54)
Dilatation 4 (8%) 3 (13%) 2 (9%) 4 (7%)
Inflammation, Chronic 9 (17%) 3 (13%) 1 (4%) 8 (15%)
Epithelium, Hyperplasia 1 (4%)
Testes (54) (22) (22) (54)
Inflammation, Chronic 1 (2%)
Mineralization 1 (2%) 1 (5%) 1 (5%) 3 (6%)
Necrosis, Multifocal 1 (2%)
Germinal Epithelium, Degeneration 3 (6%) 1 (5%) 1 (5%) 5 (9%)
Interstitial Cell, Hyperplasia 1 (2%)
Tunic, Proliferation Connective Tissue 1 (5%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 12
NTP Experiment-Test: 05025-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ETHYLENE GLYCOL Date: 09/04/94
Route: DOSED FEED Time: 06:04:38
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE UNTREATD 0.625% 1.25% 2.5%
CONTROL
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Blood (1) (3) (1)
Neutrophilia 1 (100%)
Bone Marrow (54) (21) (22) (54)
Atypia Cellular, Focal 1 (2%)
Hyperplasia, Neutrophil 1 (2%) 1 (5%)
Lymph Node (54) (33) (27) (54)
Iliac, Hyperplasia 1 (2%) 1 (4%) 3 (6%)
Inguinal, Hyperplasia 2 (4%) 1 (3%) 4 (15%) 1 (2%)
Inguinal, Hyperplasia, Lymphoid 2 (4%)
Inguinal, Inflammation, Chronic 1 (2%) 1 (2%)
Mesenteric, Angiectasis 19 (35%) 17 (52%) 11 (41%) 22 (41%)
Mesenteric, Depletion Lymphoid 1 (2%)
Mesenteric, Hematopoietic Cell Proliferation 1 (2%)
Mesenteric, Hyperplasia 3 (6%)
Mesenteric, Hyperplasia, Lymphoid 1 (2%) 2 (6%) 2 (4%)
Mesenteric, Hyperplasia, Reticulum Cell 1 (2%)
Mesenteric, Inflammation, Chronic,
Granulomatous 1 (2%)
Mesenteric, Inflammation, Suppurative 1 (2%)
Mesenteric, Thrombosis 1 (2%)
Lymph Node, Mandibular (45) (19) (17) (51)
Angiectasis 2 (4%)
Depletion Lymphoid 1 (2%)
Hyperplasia, Lymphoid 1 (2%) 4 (8%)
Spleen (54) (53) (53) (53)
Angiectasis 1 (2%)
Congestion 1 (2%)
Depletion Cellular 1 (2%) 3 (6%) 1 (2%)
Hematopoietic Cell Proliferation 17 (31%) 25 (47%) 25 (47%) 28 (53%)
Hyperplasia, Lymphoid 1 (2%) 1 (2%) 2 (4%)
Thymus (53) (19) (19) (51)
Atypia Cellular 1 (2%)
Cyst 1 (2%) 1 (5%) 1 (2%)
Degeneration, Cystic 1 (2%)
Depletion Lymphoid 1 (2%) 2 (11%) 3 (16%) 7 (14%)
Hyperplasia, Reticulum Cell 1 (2%)
Epithelial Cell, Hyperplasia, Cystic 1 (2%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Skin (54) (45) (43) (54)
Erosion 1 (2%)
Exudate 1 (2%) 1 (2%)
Fibrosis 1 (2%)
Hyperplasia, Melanocyte 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 13
NTP Experiment-Test: 05025-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ETHYLENE GLYCOL Date: 09/04/94
Route: DOSED FEED Time: 06:04:38
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE UNTREATD 0.625% 1.25% 2.5%
CONTROL
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM - CONT
Inflammation, Chronic 25 (46%) 24 (53%) 27 (63%) 18 (33%)
Metaplasia, Osseous 1 (2%) 1 (2%)
Ulcer 4 (7%) 5 (11%) 5 (12%) 9 (17%)
Hair Follicle, Atrophy 1 (2%) 1 (2%) 2 (5%)
Prepuce, Angiectasis 1 (2%)
Prepuce, Fibrosis 1 (2%)
Prepuce, Foreign Body 1 (2%) 1 (2%)
Prepuce, Inflammation, Chronic 5 (9%) 1 (2%) 2 (5%) 2 (4%)
Prepuce, Ulcer 5 (9%) 1 (2%) 3 (7%) 3 (6%)
Subcutaneous Tissue, Edema 1 (2%) 1 (2%) 1 (2%)
Subcutaneous Tissue, Exudate 1 (2%)
Subcutaneous Tissue, Fibrosis 1 (2%) 1 (2%)
Subcutaneous Tissue, Foreign Body 1 (2%) 1 (2%)
Subcutaneous Tissue, Inflammation, Chronic 1 (2%)
Subcutaneous Tissue, Lymphatic, Angiectasis 1 (2%)
Tail, Subcutaneous Tissue, Inflammation,
Granulomatous 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (54) (21) (22) (54)
Distal, Joint, Femur, Arthrosis 1 (2%) 1 (5%)
Femur, Hyperostosis 1 (2%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (54) (21) (22) (54)
Congestion 1 (2%)
Gliosis 1 (2%)
Infiltration Cellular, Mixed Cell 1 (5%)
Inflammation, Focal 1 (5%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (54) (25) (32) (54)
Bacterium 2 (4%)
Congestion 5 (9%) 2 (8%) 3 (9%) 3 (6%)
Edema 1 (4%)
Foreign Body 1 (2%)
Hemorrhage 2 (4%)
Hyperplasia, Lymphoid 4 (7%) 2 (6%) 4 (7%)
Hyperplasia, Macrophage 1 (2%) 2 (6%) 1 (2%)
Infiltration Cellular, Mixed Cell 8 (15%) 2 (8%) 7 (22%) 8 (15%)
Inflammation, Granulomatous 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 14
NTP Experiment-Test: 05025-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ETHYLENE GLYCOL Date: 09/04/94
Route: DOSED FEED Time: 06:04:38
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE UNTREATD 0.625% 1.25% 2.5%
CONTROL
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM - CONT
Pigmentation 1 (2%)
Alveolar Epithelium, Hyperplasia 2 (4%) 2 (6%)
Artery, Embolus 1 (2%)
Interstitium, Inflammation 1 (4%) 1 (2%)
Nose (54) (21) (21) (54)
Lumen, Exudate 2 (4%) 2 (10%)
Lumen, Foreign Body 1 (5%)
Nasolacrimal Duct, Foreign Body 1 (2%)
Nasolacrimal Duct, Inflammation, Suppurative 2 (4%) 1 (5%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
None
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (54) (53) (53) (54)
Angiectasis 1 (2%)
Atrophy, Focal 1 (2%) 1 (2%)
Bacterium 2 (4%) 1 (2%) 4 (7%)
Congestion 3 (6%) 1 (2%)
Hematopoietic Cell Proliferation 1 (2%)
Infiltration Cellular, Mixed Cell 1 (2%)
Inflammation, Suppurative 4 (7%) 1 (2%) 1 (2%) 4 (7%)
Metaplasia, Osseous 1 (2%) 1 (2%) 2 (4%) 1 (2%)
Mineralization 1 (2%)
Necrosis 1 (2%)
Necrosis, Focal 2 (4%)
Nephropathy 39 (72%) 44 (83%) 41 (77%) 38 (70%)
Papilla, Necrosis 1 (2%) 1 (2%)
Pelvis, Crystals 1 (2%)
Pelvis, Dilatation 2 (4%)
Pelvis, Inflammation, Chronic 2 (4%)
Pelvis, Mineralization 2 (4%)
Pelvis, Transitional Epithelium, Hyperplasia 1 (2%)
Renal Tubule, Crystals 8 (15%)
Renal Tubule, Degeneration, Hyaline 1 (2%) 1 (2%)
Renal Tubule, Dilatation 6 (11%) 2 (4%) 7 (13%) 13 (24%)
Renal Tubule, Hyperplasia 1 (2%)
Renal Tubule, Mineralization 1 (2%) 1 (2%)
Renal Tubule, Necrosis 1 (2%)
Renal Tubule, Vacuolization Cytoplasmic 51 (94%) 50 (94%) 48 (91%) 52 (96%)
Renal Tubule, Epithelium, Necrosis 2 (4%) 1 (2%)
Urethra (1) (4) (2) (18)
Fibrosis 1 (25%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 15
NTP Experiment-Test: 05025-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ETHYLENE GLYCOL Date: 09/04/94
Route: DOSED FEED Time: 06:04:38
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE UNTREATD 0.625% 1.25% 2.5%
CONTROL
____________________________________________________________________________________________________________________________________
URINARY SYSTEM - CONT
Hemorrhage 1 (6%)
Inflammation, Suppurative 1 (100%) 2 (50%) 2 (100%) 7 (39%)
Perforation 1 (100%)
Bulbourethral Gland, Dilatation 2 (50%) 1 (6%)
Bulbourethral Gland, Fibrosis 1 (25%)
Lumen, Crystals 12 (67%)
Urinary Bladder (53) (53) (53) (54)
Calculus Gross Observation 2 (4%)
Calculus Micro Observation Only 2 (4%)
Congestion 1 (2%)
Hemorrhage 1 (2%) 1 (2%)
Hyperplasia, Lymphoid 1 (2%) 2 (4%) 1 (2%)
Inflammation, Chronic 1 (2%) 4 (8%) 6 (11%) 8 (15%)
Ulcer 1 (2%)
Artery, Serosa, Mineralization 1 (2%)
Transitional Epithelium, Hyperplasia 1 (2%) 1 (2%) 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 16
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