TDMS Study 50215-03 Pathology Tables
NTP Experiment-Test: 50215-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00
Route: GAVAGE Time: 12:36:12
MICE FINAL#1
Facility: Battelle Columbus Laboratory
Chemical CAS #: 107-13-1
Lock Date: 11/03/99
Cage Range: All
Reasons For Removal: All
Removal Date Range: All
Treatment Groups: Include All
a Number of animals examined microscopically at site and number of animals with lesion
Page 1
NTP Experiment-Test: 50215-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00
Route: GAVAGE Time: 12:36:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 MG/KG 2.5 10 MG/KG 20 MG/KG
MG/KG
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 50 50 50 50
Early Deaths
Natural Death 6 15 10 25
Moribund Sacrifice 5 3 1 1
Dosing Accident 1
Survivors
Terminal Sacrifice 39 32 39 22
Moribund Sacrifice 1
Animals Examined Microscopically 50 50 50 50
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Esophagus (50) (50) (49) (50)
Perforation 1 (2%)
Gallbladder (50) (50) (49) (50)
Infiltration Cellular, Lymphocyte 1 (2%)
Intestine Small, Jejunum (50) (50) (50) (50)
Inflammation, Suppurative 1 (2%)
Peyer's Patch, Hyperplasia, Lymphoid 1 (2%) 1 (2%)
Liver (50) (50) (50) (50)
Angiectasis 1 (2%) 3 (6%) 9 (18%) 8 (16%)
Basophilic Focus 1 (2%) 6 (12%) 4 (8%) 6 (12%)
Clear Cell Focus 5 (10%) 3 (6%) 6 (12%) 5 (10%)
Eosinophilic Focus 9 (18%) 7 (14%) 18 (36%) 11 (22%)
Hematopoietic Cell Proliferation 1 (2%) 4 (8%) 3 (6%) 1 (2%)
Hyperplasia 1 (2%)
Infarct 1 (2%)
Inflammation, Chronic Active 42 (84%) 34 (68%) 40 (80%) 32 (64%)
Mineralization 1 (2%) 1 (2%)
Mixed Cell Focus 3 (6%) 15 (30%) 16 (32%) 13 (26%)
Necrosis 1 (2%) 3 (6%) 3 (6%) 6 (12%)
Pigmentation 6 (12%) 18 (36%) 13 (26%) 1 (2%)
Vacuolization Cytoplasmic 9 (18%) 12 (24%) 15 (30%) 21 (42%)
Bile Duct, Cyst 1 (2%)
Centrilobular, Degeneration 1 (2%)
Portal, Infiltration Cellular, Lymphocyte 2 (4%)
Mesentery (9) (10) (10) (10)
Fibrosis 1 (11%)
Fat, Inflammation, Chronic Active 1 (11%) 1 (10%) 1 (10%)
Fat, Necrosis 5 (56%) 7 (70%) 8 (80%) 6 (60%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 2
NTP Experiment-Test: 50215-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00
Route: GAVAGE Time: 12:36:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 MG/KG 2.5 10 MG/KG 20 MG/KG
MG/KG
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Pancreas (50) (50) (50) (50)
Fibrosis 1 (2%)
Infiltration Cellular, Mononuclear Cell 2 (4%) 1 (2%) 9 (18%)
Inflammation, Chronic Active 1 (2%)
Acinus, Atrophy 2 (4%)
Salivary Glands (50) (50) (49) (50)
Infiltration Cellular, Mononuclear Cell 36 (72%) 32 (64%) 32 (65%) 15 (30%)
Stomach, Forestomach (50) (50) (50) (50)
Erosion, Focal 1 (2%)
Hyperkeratosis, Diffuse 1 (2%) 2 (4%) 3 (6%)
Hyperkeratosis, Focal 1 (2%) 1 (2%) 1 (2%)
Inflammation, Chronic Active, Focal 1 (2%) 2 (4%) 1 (2%)
Inflammation, Chronic Active, Multifocal 1 (2%) 1 (2%)
Ulcer, Multifocal 1 (2%) 1 (2%)
Epithelium, Hyperplasia, Focal 2 (4%) 1 (2%) 5 (10%) 7 (14%)
Epithelium, Hyperplasia, Multifocal 1 (2%)
Stomach, Glandular (50) (50) (50) (50)
Hyperplasia, Focal 1 (2%) 1 (2%)
Inflammation, Chronic Active 1 (2%)
Mineralization 2 (4%) 1 (2%)
Glands, Ectasia 11 (22%) 11 (22%) 6 (12%) 6 (12%)
Tooth (1)
Malformation 1 (100%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Blood Vessel (50) (50) (50) (50)
Aorta, Mineralization 1 (2%) 1 (2%) 1 (2%)
Heart (50) (50) (50) (50)
Inflammation, Chronic Active 1 (2%) 1 (2%) 3 (6%)
Mineralization 1 (2%) 1 (2%) 1 (2%)
Thrombosis 1 (2%)
Valve, Inflammation, Suppurative 1 (2%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (50) (50) (50) (50)
Accessory Adrenal Cortical Nodule 1 (2%)
Cyst 1 (2%)
Hematopoietic Cell Proliferation 1 (2%) 2 (4%)
Hyperplasia 1 (2%) 1 (2%) 1 (2%) 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 3
NTP Experiment-Test: 50215-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00
Route: GAVAGE Time: 12:36:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 MG/KG 2.5 10 MG/KG 20 MG/KG
MG/KG
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM - CONT
Hypertrophy 1 (2%)
Inflammation, Chronic Active 1 (2%)
Metaplasia, Osseous 1 (2%)
Subcapsular, Hyperplasia 50 (100%) 49 (98%) 47 (94%) 49 (98%)
Adrenal Medulla (50) (50) (50) (50)
Hyperplasia 1 (2%)
Parathyroid Gland (43) (39) (37) (34)
Cyst 1 (2%) 1 (3%)
Pituitary Gland (48) (49) (48) (49)
Pars Distalis, Angiectasis 1 (2%) 1 (2%)
Pars Distalis, Cyst 1 (2%)
Pars Distalis, Hyperplasia 10 (21%) 8 (16%) 6 (13%) 2 (4%)
Pars Distalis, Pigmentation 1 (2%)
Thyroid Gland (50) (50) (49) (50)
Infiltration Cellular, Lymphocyte 1 (2%)
Follicle, Cyst 6 (12%) 4 (8%) 3 (6%) 1 (2%)
Follicular Cell, Hyperplasia 1 (2%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Clitoral Gland (50) (48) (49) (48)
Infiltration Cellular, Mononuclear Cell 2 (4%)
Inflammation, Chronic Active 3 (6%) 1 (2%) 1 (2%)
Pigmentation 1 (2%)
Duct, Cyst 1 (2%)
Ovary (50) (50) (50) (50)
Angiectasis 1 (2%) 1 (2%) 2 (4%)
Atrophy 6 (12%) 8 (16%) 45 (90%) 40 (80%)
Cyst 12 (24%) 20 (40%) 27 (54%) 19 (38%)
Mineralization 2 (4%) 2 (4%)
Pigmentation 1 (2%) 3 (6%) 2 (4%)
Thrombosis 2 (4%)
Uterus (50) (50) (50) (50)
Angiectasis 1 (2%) 2 (4%) 3 (6%) 1 (2%)
Cyst 1 (2%)
Inflammation, Suppurative 1 (2%) 2 (4%)
Endometrium, Hyperplasia, Cystic 40 (80%) 41 (82%) 29 (58%) 19 (38%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 4
NTP Experiment-Test: 50215-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00
Route: GAVAGE Time: 12:36:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 MG/KG 2.5 10 MG/KG 20 MG/KG
MG/KG
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM - CONT
Lymphatic, Angiectasis 1 (2%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (50) (50) (50) (50)
Myelofibrosis 1 (2%) 1 (2%) 1 (2%)
Myeloid Cell, Hyperplasia 1 (2%) 5 (10%) 3 (6%) 5 (10%)
Lymph Node (37) (32) (30) (25)
Inguinal, Hyperplasia, Lymphoid 1 (3%)
Mediastinal, Hematopoietic Cell Proliferation 1 (3%)
Mediastinal, Hyperplasia, Lymphoid 2 (5%) 4 (13%) 5 (17%) 4 (16%)
Renal, Hematopoietic Cell Proliferation 1 (3%)
Renal, Hyperplasia, Lymphoid 1 (3%)
Lymph Node, Mandibular (49) (49) (47) (50)
Angiectasis 1 (2%)
Hematopoietic Cell Proliferation 1 (2%)
Hyperplasia, Lymphoid 4 (8%) 7 (14%) 12 (26%) 9 (18%)
Lymph Node, Mesenteric (50) (49) (50) (47)
Angiectasis 1 (2%)
Ectasia 1 (2%) 2 (4%)
Hematopoietic Cell Proliferation 1 (2%) 1 (2%) 1 (2%)
Hyperplasia, Lymphoid 3 (6%) 1 (2%) 3 (6%) 1 (2%)
Hyperplasia, Plasma Cell 1 (2%)
Infiltration Cellular, Polymorphonuclear 1 (2%) 1 (2%)
Inflammation, Chronic Active 1 (2%) 2 (4%)
Spleen (50) (50) (50) (50)
Hematopoietic Cell Proliferation 17 (34%) 23 (46%) 24 (48%) 21 (42%)
Hyperplasia, Lymphoid 18 (36%) 16 (32%) 17 (34%) 10 (20%)
Thymus (50) (50) (47) (50)
Atrophy 6 (12%) 11 (22%) 4 (9%) 10 (20%)
Ectopic Parathyroid Gland 1 (2%) 2 (4%)
Hyperplasia, Lymphoid 1 (2%) 2 (4%)
Thymocyte, Necrosis 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Mammary Gland (50) (50) (50) (50)
Hyperplasia 1 (2%)
Skin (50) (50) (50) (50)
Inflammation, Chronic Active 1 (2%) 1 (2%)
Ulcer 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 5
NTP Experiment-Test: 50215-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00
Route: GAVAGE Time: 12:36:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 MG/KG 2.5 10 MG/KG 20 MG/KG
MG/KG
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM - CONT
Epidermis, Hyperplasia 1 (2%)
Subcutaneous Tissue, Fibrosis 1 (2%) 2 (4%) 1 (2%)
Subcutaneous Tissue, Hemorrhage 1 (2%)
Subcutaneous Tissue, Infiltration Cellular,
Mast Cell 1 (2%)
Subcutaneous Tissue, Inflammation, Chronic
Active 2 (4%) 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Skeletal Muscle (3) (3) (2) (1)
Inflammation, Chronic Active 1 (33%)
Arteriole, Mineralization 1 (33%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
None
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (50) (50) (50) (50)
Erythrophagocytosis 1 (2%) 1 (2%)
Fibrosis 1 (2%)
Hemorrhage 1 (2%)
Inflammation, Chronic Active 1 (2%) 3 (6%) 1 (2%) 1 (2%)
Inflammation, Suppurative 2 (4%) 2 (4%)
Mineralization 1 (2%) 1 (2%)
Necrosis 1 (2%)
Pigmentation 1 (2%) 1 (2%)
Thrombosis 2 (4%)
Alveolar Epithelium, Hyperplasia 2 (4%) 2 (4%) 4 (8%)
Alveolus, Infiltration Cellular, Histiocyte 2 (4%) 3 (6%) 4 (8%) 4 (8%)
Mediastinum, Inflammation, Chronic Active 1 (2%)
Nose (50) (50) (50) (50)
Nasolacrimal Duct, Inflammation, Suppurative 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Eye (1) (2) (9)
a Number of animals examined microscopically at site and number of animals with lesion
Page 6
NTP Experiment-Test: 50215-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00
Route: GAVAGE Time: 12:36:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0 MG/KG 2.5 10 MG/KG 20 MG/KG
MG/KG
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM - CONT
Atrophy 1 (100%) 1 (50%) 7 (78%)
Synechia 1 (50%) 1 (11%)
Cornea, Inflammation, Chronic Active 1 (100%) 5 (56%)
Lens, Cataract 2 (22%)
Retina, Degeneration 1 (50%)
Harderian Gland (49) (50) (49) (49)
Hyperplasia 5 (10%) 4 (8%) 6 (12%) 7 (14%)
Infiltration Cellular, Mononuclear Cell 40 (82%) 30 (60%) 33 (67%) 23 (47%)
Bilateral, Hyperplasia 1 (2%)
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (50) (50) (50) (50)
Infarct 2 (4%) 3 (6%)
Inflammation, Chronic Active 2 (4%) 2 (4%)
Metaplasia, Osseous 2 (4%) 1 (2%) 1 (2%)
Mineralization 4 (8%) 3 (6%) 2 (4%) 4 (8%)
Nephropathy 14 (28%) 6 (12%) 10 (20%) 5 (10%)
Cortex, Cyst 2 (4%) 2 (4%) 1 (2%)
Cortex, Hydronephrosis 1 (2%)
Medulla, Cyst 1 (2%)
Renal Tubule, Hyperplasia 1 (2%)
Renal Tubule, Vacuolization Cytoplasmic 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 7
NTP Experiment-Test: 50215-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00
Route: GAVAGE Time: 12:36:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 MG/KG 2.5 10 MG/KG 20 MG/KG
MG/KG
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 50 50 50 50
Early Deaths
Natural Death 11 3 8 32
Moribund Sacrifice 1 3 3 4
Dosing Accident 2
Survivors
Terminal Sacrifice 38 42 39 14
Animals Examined Microscopically 50 50 50 50
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Esophagus (50) (50) (50) (50)
Inflammation, Suppurative 1 (2%)
Perforation 2 (4%)
Gallbladder (50) (49) (50) (48)
Cyst 1 (2%)
Intestine Large, Cecum (50) (50) (50) (50)
Inflammation, Chronic Active 1 (2%)
Ulcer 1 (2%)
Intestine Small, Duodenum (50) (50) (50) (50)
Ulcer 1 (2%)
Intestine Small, Jejunum (50) (50) (50) (50)
Inflammation, Chronic Active 1 (2%)
Peyer's Patch, Hyperplasia, Lymphoid 2 (4%) 1 (2%)
Liver (50) (50) (50) (50)
Angiectasis 1 (2%) 2 (4%) 5 (10%) 1 (2%)
Basophilic Focus 1 (2%) 5 (10%) 5 (10%) 4 (8%)
Clear Cell Focus 19 (38%) 24 (48%) 16 (32%) 4 (8%)
Eosinophilic Focus 5 (10%) 5 (10%) 10 (20%) 4 (8%)
Fibrosis 1 (2%) 1 (2%)
Hematopoietic Cell Proliferation 2 (4%) 2 (4%) 1 (2%) 1 (2%)
Infarct 2 (4%) 1 (2%) 2 (4%)
Inflammation, Chronic Active 25 (50%) 33 (66%) 33 (66%) 11 (22%)
Mineralization 1 (2%) 1 (2%)
Mixed Cell Focus 9 (18%) 13 (26%) 13 (26%) 3 (6%)
Necrosis 4 (8%) 10 (20%) 5 (10%)
Pigmentation 4 (8%) 3 (6%)
Vacuolization Cytoplasmic 18 (36%) 30 (60%) 23 (46%) 17 (34%)
Centrilobular, Degeneration 1 (2%)
Mesentery (8) (6) (8) (3)
a Number of animals examined microscopically at site and number of animals with lesion
Page 8
NTP Experiment-Test: 50215-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00
Route: GAVAGE Time: 12:36:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 MG/KG 2.5 10 MG/KG 20 MG/KG
MG/KG
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Artery, Inflammation, Chronic Active 1 (13%)
Artery, Necrosis, Fibrinoid 1 (13%)
Fat, Inflammation, Chronic Active 1 (13%)
Fat, Mineralization 2 (25%)
Fat, Necrosis 2 (25%) 3 (50%) 4 (50%) 2 (67%)
Oral Mucosa (2) (4) (1)
Gingival, Foreign Body 2 (100%) 3 (75%)
Gingival, Inflammation, Suppurative 2 (100%) 4 (100%) 1 (100%)
Pancreas (50) (50) (50) (50)
Infiltration Cellular, Mononuclear Cell 1 (2%) 2 (4%) 1 (2%)
Inflammation, Chronic Active 1 (2%)
Acinus, Atrophy 1 (2%) 1 (2%)
Salivary Glands (50) (50) (50) (50)
Infiltration Cellular, Mononuclear Cell 35 (70%) 34 (68%) 36 (72%) 23 (46%)
Duct, Cyst 1 (2%)
Stomach, Forestomach (50) (50) (50) (50)
Hyperkeratosis, Diffuse 3 (6%) 6 (12%)
Hyperkeratosis, Focal 2 (4%) 3 (6%) 4 (8%) 6 (12%)
Hyperplasia 1 (2%)
Inflammation, Chronic Active 1 (2%)
Inflammation, Chronic Active, Focal 1 (2%) 2 (4%) 2 (4%)
Inflammation, Chronic Active, Multifocal 1 (2%) 1 (2%)
Ulcer 1 (2%) 1 (2%) 1 (2%)
Ulcer, Multifocal 2 (4%) 1 (2%)
Epithelium, Hyperplasia, Focal 2 (4%) 4 (8%) 8 (16%) 9 (18%)
Stomach, Glandular (50) (50) (50) (50)
Hyperplasia, Focal 1 (2%) 1 (2%) 1 (2%)
Inflammation, Chronic Active 2 (4%)
Mineralization 1 (2%)
Ulcer 1 (2%)
Glands, Ectasia 2 (4%) 7 (14%) 11 (22%) 4 (8%)
Tooth (9) (5) (4) (3)
Inflammation, Suppurative 1 (25%)
Malformation 9 (100%) 5 (100%) 3 (75%) 2 (67%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Blood Vessel (50) (50) (50) (50)
Aorta, Mineralization 1 (2%)
Heart (50) (50) (50) (50)
Inflammation, Chronic Active 1 (2%) 3 (6%) 2 (4%) 1 (2%)
Inflammation, Suppurative 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 9
NTP Experiment-Test: 50215-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00
Route: GAVAGE Time: 12:36:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 MG/KG 2.5 10 MG/KG 20 MG/KG
MG/KG
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM - CONT
Mineralization 2 (4%) 1 (2%)
Valve, Inflammation, Chronic Active 1 (2%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (50) (50) (50) (50)
Hypertrophy 19 (38%) 21 (42%) 19 (38%) 2 (4%)
Subcapsular, Cyst Multilocular 1 (2%)
Subcapsular, Hyperplasia 45 (90%) 43 (86%) 40 (80%) 36 (72%)
Adrenal Medulla (50) (50) (50) (50)
Hyperplasia 1 (2%) 1 (2%)
Parathyroid Gland (42) (44) (34) (41)
Cyst 2 (5%) 1 (2%)
Pituitary Gland (49) (46) (47) (47)
Pars Distalis, Cyst 3 (6%) 2 (4%) 3 (6%) 2 (4%)
Thyroid Gland (50) (50) (50) (50)
Follicle, Cyst 3 (6%) 3 (6%) 5 (10%) 1 (2%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Epididymis (50) (50) (50) (50)
Atrophy 1 (2%) 1 (2%)
Granuloma Sperm 1 (2%) 1 (2%)
Infiltration Cellular, Mononuclear Cell 42 (84%) 31 (62%) 38 (76%) 16 (32%)
Inflammation, Chronic Active 2 (4%) 1 (2%)
Inflammation, Suppurative 1 (2%)
Mineralization 1 (2%)
Preputial Gland (49) (50) (50) (50)
Hyperplasia, Basal Cell 1 (2%)
Infiltration Cellular, Mononuclear Cell 11 (22%) 13 (26%) 11 (22%) 13 (26%)
Inflammation, Chronic Active 8 (16%) 3 (6%) 8 (16%) 2 (4%)
Bilateral, Inflammation, Chronic Active 1 (2%)
Bilateral, Duct, Ectasia 4 (8%) 1 (2%) 2 (4%) 1 (2%)
Duct, Ectasia 13 (27%) 10 (20%) 20 (40%) 15 (30%)
Prostate (50) (50) (50) (50)
Infiltration Cellular, Mononuclear Cell 36 (72%) 31 (62%) 29 (58%) 22 (44%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 10
NTP Experiment-Test: 50215-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00
Route: GAVAGE Time: 12:36:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 MG/KG 2.5 10 MG/KG 20 MG/KG
MG/KG
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM - CONT
Inflammation, Chronic Active 2 (4%) 2 (4%)
Metaplasia, Mucous 1 (2%)
Mineralization 1 (2%)
Seminal Vesicle (50) (50) (50) (50)
Atrophy 1 (2%)
Inflammation, Chronic Active 1 (2%)
Testes (50) (50) (50) (50)
Inflammation, Suppurative 1 (2%)
Mineralization 2 (4%) 1 (2%) 2 (4%)
Necrosis 1 (2%)
Germinal Epithelium, Degeneration 2 (4%) 1 (2%) 3 (6%) 1 (2%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (50) (50) (50) (50)
Angiectasis 2 (4%)
Atrophy 1 (2%)
Inflammation, Suppurative 1 (2%)
Myeloid Cell, Hyperplasia 1 (2%) 4 (8%) 2 (4%)
Lymph Node (6) (4) (6) (5)
Mediastinal, Hyperplasia, Lymphoid 1 (25%) 1 (17%)
Pancreatic, Hyperplasia, Lymphoid 1 (20%)
Lymph Node, Mandibular (49) (47) (47) (47)
Hematopoietic Cell Proliferation 1 (2%)
Hyperplasia, Lymphoid 1 (2%) 1 (2%) 5 (11%) 4 (9%)
Lymph Node, Mesenteric (48) (48) (49) (48)
Angiectasis 1 (2%)
Atrophy 1 (2%)
Ectasia 2 (4%)
Hematopoietic Cell Proliferation 1 (2%) 2 (4%)
Hyperplasia, Lymphoid 4 (8%) 1 (2%)
Hyperplasia, Plasma Cell 1 (2%)
Inflammation, Chronic Active 1 (2%) 1 (2%)
Spleen (50) (50) (50) (50)
Depletion Cellular 1 (2%)
Fibrosis 1 (2%)
Hematopoietic Cell Proliferation 17 (34%) 7 (14%) 16 (32%) 12 (24%)
Hyperplasia, Lymphoid 2 (4%)
Lymphoid Follicle, Depletion Cellular 1 (2%)
Red Pulp, Depletion Cellular 1 (2%)
Thymus (47) (47) (44) (46)
Atrophy 7 (15%) 4 (9%) 7 (16%) 4 (9%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 11
NTP Experiment-Test: 50215-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00
Route: GAVAGE Time: 12:36:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 MG/KG 2.5 10 MG/KG 20 MG/KG
MG/KG
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM - CONT
Inflammation, Chronic Active 1 (2%)
Thymocyte, Necrosis 1 (2%) 3 (7%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Skin (50) (50) (50) (50)
Infiltration Cellular, Mononuclear Cell 1 (2%) 1 (2%)
Inflammation, Chronic Active 1 (2%)
Ulcer 1 (2%)
Subcutaneous Tissue, Infiltration Cellular,
Mast Cell 1 (2%)
Subcutaneous Tissue, Inflammation,
Suppurative 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (50) (50) (50) (50)
Hyperostosis 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (50) (50) (50) (50)
Cyst Epithelial Inclusion 1 (2%)
Hemorrhage 2 (4%)
Inflammation, Chronic Active 1 (2%)
Thrombosis 1 (2%)
Vacuolization Cytoplasmic 1 (2%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (50) (50) (50) (50)
Erythrophagocytosis 1 (2%) 2 (4%)
Inflammation, Chronic Active 4 (8%) 1 (2%) 3 (6%)
Inflammation, Granulomatous 1 (2%)
Inflammation, Suppurative 2 (4%) 1 (2%)
Metaplasia, Osseous 1 (2%)
Mineralization 1 (2%)
Necrosis 1 (2%)
Pigmentation 1 (2%) 2 (4%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 12
NTP Experiment-Test: 50215-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACRYLONITRILE Date: 10/31/00
Route: GAVAGE Time: 12:36:12
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0 MG/KG 2.5 10 MG/KG 20 MG/KG
MG/KG
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM - CONT
Alveolar Epithelium, Hyperplasia 4 (8%) 3 (6%) 5 (10%) 6 (12%)
Alveolus, Infiltration Cellular, Histiocyte 1 (2%) 4 (8%)
Artery, Mediastinum, Mineralization 1 (2%)
Mediastinum, Hemorrhage 1 (2%)
Mediastinum, Inflammation, Chronic Active 1 (2%)
Nose (50) (50) (50) (50)
Foreign Body 1 (2%)
Inflammation, Suppurative 1 (2%) 1 (2%)
Nasolacrimal Duct, Inflammation, Suppurative 4 (8%) 1 (2%) 4 (8%) 7 (14%)
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SPECIAL SENSES SYSTEM
Eye (1) (6) (3)
Atrophy 1 (100%) 4 (67%) 2 (67%)
Synechia 2 (33%) 2 (67%)
Cornea, Inflammation, Chronic Active 3 (50%) 3 (100%)
Cornea, Sclera, Inflammation, Chronic Active 1 (17%)
Lens, Cataract 1 (100%) 3 (50%) 2 (67%)
Retina, Atrophy 1 (17%)
Harderian Gland (50) (50) (50) (50)
Hyperplasia 1 (2%) 4 (8%) 7 (14%) 4 (8%)
Infiltration Cellular, Mononuclear Cell 33 (66%) 25 (50%) 26 (52%) 14 (28%)
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URINARY SYSTEM
Kidney (50) (50) (50) (50)
Accumulation, Hyaline Droplet 1 (2%)
Infarct 5 (10%) 3 (6%) 3 (6%) 5 (10%)
Inflammation, Chronic Active 2 (4%) 3 (6%) 3 (6%) 2 (4%)
Inflammation, Suppurative 1 (2%)
Metaplasia, Osseous 5 (10%) 2 (4%) 5 (10%) 3 (6%)
Mineralization 23 (46%) 28 (56%) 32 (64%) 18 (36%)
Necrosis 1 (2%)
Nephropathy 41 (82%) 39 (78%) 42 (84%) 19 (38%)
Artery, Inflammation, Chronic Active 1 (2%)
Cortex, Cyst 10 (20%) 13 (26%) 13 (26%) 4 (8%)
Glomerulus, Thrombosis 1 (2%)
Pelvis, Inflammation, Suppurative 1 (2%)
Renal Tubule, Casts Protein 1 (2%)
Renal Tubule, Hyperplasia 5 (10%) 5 (10%) 4 (8%)
a Number of animals examined microscopically at site and number of animals with lesion
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