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TDMS Study 89029-12 Pathology Tables

NTP                                    STATISTICAL ANALYSIS OF NON-NEOPLASTIC LESIONS                          REPORT: PEIRPT10   
LAB: Battelle Northwest                        MAGNETIC FIELDS + DMBA INITIATION PROMOTION                     DATE: 01/07/98     
EXPERIMENT: 89029 TEST: 12                                                                                     TIME: 16:17:51     
TEST TYPE:  SUBCHRON 90-DAY                     CAGES FROM 0000 TO LAST CAGE                                   PAGE:              
CONT: N01-ES-65397                              ROUTE: MAGNETIC FIELDS                                         NTP C#: 89029D     
PATHOLOGIST: GRUMBEIN, S.                                                                                      CAS: EMF+DMBA      



SUMMARY OF STATISTICALLY SIGNIFICANT (P<=.05) RESULTS
IN THE ANALYSIS OF    MAGNETIC FIELDS + DMBA INITIATION PROMOTIO
===============================================================

Female Rats
------------
Organ                                  Morphology
-----                                  ----------
Lung: Alveolar Epithelium              Hyperplasia
Lung: Alveolus                         Infiltration Cellular Histiocyte
===============================================================
       Date:  01/07/98                           EXPERIMENT: 89029 TEST: 12                                            Page 1

Statistical Analysis of Non-neoplastic Lesions in        Rats(SPRAGUE-DAWLEY)  -     MAGNETIC FIELDS + DMBA INITIATION PROMOTIO
                                         Terminal Sacrifice at 12 weeks 
|=================================================================================================================================|
|                   |                                                                                                             |
|                   |                                                    Females                                                  |
|Dose               |        20 DMBA          50 HZ/1G         50 HZ/5G                                                           |
|                   |        CONTROL           20 DMBA          20 DMBA                                                           |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney                                                                                                                           |
|     Mineralization                                                                                                              |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                                                                             |
|-----------        |                                                                                                             |
|OVERALL (a)        |        2/100 (2%)       0/100 (0%)       1/100 (1%)                                                         |
|POLY-3 ADJUSTED (b)|        2.0%             0.0%             1.0%                                                               |
|TERMINAL (d)       |        1/94 (1%)        0/87 (0%)        1/92 (1%)                                                          |
|FIRST INCIDENCE    |        72               ---              90 (T)                                                             |
|-------------------|                                                                                                             |
|STATISTICAL TESTS  |                                                                                                             |
|-------------------|                                                                                                             |
|LIFE TABLE         |        P=0.606N         P=0.251N         P=0.507N                                                           |
|POLY 3             |        P=0.601N         P=0.245N         P=0.505N                                                           |
|POLY 1.5           |        P=0.603N         P=0.243N         P=0.503N                                                           |
|POLY 6             |        P=0.599N         P=0.250N         P=0.508N                                                           |
|LOGISTIC REGRESSION|        P=0.613N         P=0.187N         P=0.486N                                                           |
|COCH-ARM / FISHERS |        P=0.606N         P=0.249N         P=0.500N                                                           |
|=================================================================================================================================|
|                   |                                                                                                             |
|                   |                                                    Females                                                  |
|Dose               |        20 DMBA          50 HZ/1G         50 HZ/5G                                                           |
|                   |        CONTROL           20 DMBA          20 DMBA                                                           |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney                                                                                                                           |
|     Nephropathy                                                                                                                 |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                                                                             |
|-----------        |                                                                                                             |
|OVERALL (a)        |        15/100 (15%)     16/100 (16%)     10/100 (10%)                                                       |
|POLY-3 ADJUSTED (b)|        15.3%            16.8%            10.4%                                                              |
|TERMINAL (d)       |        14/94 (15%)      15/87 (17%)      10/92 (11%)                                                        |
|FIRST INCIDENCE    |        72               84               90 (T)                                                             |
|-------------------|                                                                                                             |
|STATISTICAL TESTS  |                                                                                                             |
|-------------------|                                                                                                             |
|LIFE TABLE         |        P=0.138N         P=0.410          P=0.213N                                                           |
|POLY 3             |        P=0.141N         P=0.465          P=0.206N                                                           |
|POLY 1.5           |        P=0.140N         P=0.480          P=0.202N                                                           |
|POLY 6             |        P=0.143N         P=0.446          P=0.212N                                                           |
|LOGISTIC REGRESSION|        P=0.138N         P=0.470          P=0.201N                                                           |
|COCH-ARM / FISHERS |        P=0.138N         P=0.500          P=0.196N                                                           |
|=================================================================================================================================|
       Date:  01/07/98                           EXPERIMENT: 89029 TEST: 12                                            Page 2

Statistical Analysis of Non-neoplastic Lesions in       Rats(SPRAGUE-DAWLEY)  -     MAGNETIC FIELDS + DMBA INITIATION PROMOTIO
                                         Terminal Sacrifice at 12 weeks 
|=================================================================================================================================|
|                   |                                                                                                             |
|                   |                                                    Females                                                  |
|Dose               |        20 DMBA          50 HZ/1G         50 HZ/5G                                                           |
|                   |        CONTROL           20 DMBA          20 DMBA                                                           |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Clear Cell Focus                                                                                                            |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                                                                             |
|-----------        |                                                                                                             |
|OVERALL (a)        |        1/100 (1%)       0/100 (0%)       3/100 (3%)                                                         |
|POLY-3 ADJUSTED (b)|        1.0%             0.0%             3.1%                                                               |
|TERMINAL (d)       |        1/94 (1%)        0/87 (0%)        3/92 (3%)                                                          |
|FIRST INCIDENCE    |        90 (T)           ---              90 (T)                                                             |
|-------------------|                                                                                                             |
|STATISTICAL TESTS  |                                                                                                             |
|-------------------|                                                                                                             |
|LIFE TABLE         |        P=0.125          P=0.515N         P=0.300                                                            |
|POLY 3             |        P=0.122          P=0.505N         P=0.304                                                            |
|POLY 1.5           |        P=0.122          P=0.503N         P=0.305                                                            |
|POLY 6             |        P=0.123          P=0.508N         P=0.302                                                            |
|LOGISTIC REGRESSION|        P=0.125          (e)              P=0.300                                                            |
|COCH-ARM / FISHERS |        P=0.122          P=0.500N         P=0.311                                                            |
|=================================================================================================================================|
|                   |                                                                                                             |
|                   |                                                    Females                                                  |
|Dose               |        20 DMBA          50 HZ/1G         50 HZ/5G                                                           |
|                   |        CONTROL           20 DMBA          20 DMBA                                                           |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Fatty Change                                                                                                                |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                                                                             |
|-----------        |                                                                                                             |
|OVERALL (a)        |        0/100 (0%)       2/100 (2%)       1/100 (1%)                                                         |
|POLY-3 ADJUSTED (b)|        0.0%             2.1%             1.0%                                                               |
|TERMINAL (d)       |        0/94 (0%)        2/87 (2%)        0/92 (0%)                                                          |
|FIRST INCIDENCE    |        ---              90 (T)           77                                                                 |
|-------------------|                                                                                                             |
|STATISTICAL TESTS  |                                                                                                             |
|-------------------|                                                                                                             |
|LIFE TABLE         |        P=0.608          P=0.222          P=0.504                                                            |
|POLY 3             |        P=0.603          P=0.233          P=0.499                                                            |
|POLY 1.5           |        P=0.604          P=0.235          P=0.499                                                            |
|POLY 6             |        P=0.601          P=0.230          P=0.498                                                            |
|LOGISTIC REGRESSION|        P=0.604          P=0.222          P=0.514                                                            |
|COCH-ARM / FISHERS |        P=0.606          P=0.249          P=0.500                                                            |
|=================================================================================================================================|
       Date:  01/07/98                           EXPERIMENT: 89029 TEST: 12                                            Page 3

Statistical Analysis of Non-neoplastic Lesions in       Rats(SPRAGUE-DAWLEY)  -     MAGNETIC FIELDS + DMBA INITIATION PROMOTIO
                                         Terminal Sacrifice at 12 weeks 
|=================================================================================================================================|
|                   |                                                                                                             |
|                   |                                                    Females                                                  |
|Dose               |        20 DMBA          50 HZ/1G         50 HZ/5G                                                           |
|                   |        CONTROL           20 DMBA          20 DMBA                                                           |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hematopoietic Cell Proliferation                                                                                            |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                                                                             |
|-----------        |                                                                                                             |
|OVERALL (a)        |        19/100 (19%)     12/100 (12%)     12/100 (12%)                                                       |
|POLY-3 ADJUSTED (b)|        19.4%            12.6%            12.4%                                                              |
|TERMINAL (d)       |        18/94 (19%)      12/87 (14%)      12/92 (13%)                                                        |
|FIRST INCIDENCE    |        72               90 (T)           90 (T)                                                             |
|-------------------|                                                                                                             |
|STATISTICAL TESTS  |                                                                                                             |
|-------------------|                                                                                                             |
|LIFE TABLE         |        P=0.178N         P=0.172N         P=0.135N                                                           |
|POLY 3             |        P=0.179N         P=0.139N         P=0.128N                                                           |
|POLY 1.5           |        P=0.179N         P=0.131N         P=0.125N                                                           |
|POLY 6             |        P=0.180N         P=0.151N         P=0.133N                                                           |
|LOGISTIC REGRESSION|        P=0.180N         P=0.140N         P=0.125N                                                           |
|COCH-ARM / FISHERS |        P=0.180N         P=0.120N         P=0.120N                                                           |
|=================================================================================================================================|
|                   |                                                                                                             |
|                   |                                                    Females                                                  |
|Dose               |        20 DMBA          50 HZ/1G         50 HZ/5G                                                           |
|                   |        CONTROL           20 DMBA          20 DMBA                                                           |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver: Centrilobular                                                                                                             |
|     Necrosis                                                                                                                    |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                                                                             |
|-----------        |                                                                                                             |
|OVERALL (a)        |        1/100 (1%)       3/100 (3%)       1/100 (1%)                                                         |
|POLY-3 ADJUSTED (b)|        1.0%             3.1%             1.0%                                                               |
|TERMINAL (d)       |        1/94 (1%)        0/87 (0%)        0/92 (0%)                                                          |
|FIRST INCIDENCE    |        90 (T)           81               79                                                                 |
|-------------------|                                                                                                             |
|STATISTICAL TESTS  |                                                                                                             |
|-------------------|                                                                                                             |
|LIFE TABLE         |        P=0.502N         P=0.293          P=0.758                                                            |
|POLY 3             |        P=0.501N         P=0.301          P=0.759                                                            |
|POLY 1.5           |        P=0.501N         P=0.303          P=0.760                                                            |
|POLY 6             |        P=0.502N         P=0.298          P=0.758                                                            |
|LOGISTIC REGRESSION|        P=0.510N         P=0.352          P=0.754N                                                           |
|COCH-ARM / FISHERS |        P=0.500N         P=0.311          P=0.751N                                                           |
|=================================================================================================================================|
       Date:  01/07/98                           EXPERIMENT: 89029 TEST: 12                                            Page 4

Statistical Analysis of Non-neoplastic Lesions in       Rats(SPRAGUE-DAWLEY)  -     MAGNETIC FIELDS + DMBA INITIATION PROMOTIO
                                         Terminal Sacrifice at 12 weeks 
|=================================================================================================================================|
|                   |                                                                                                             |
|                   |                                                    Females                                                  |
|Dose               |        20 DMBA          50 HZ/1G         50 HZ/5G                                                           |
|                   |        CONTROL           20 DMBA          20 DMBA                                                           |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung: Alveolar Epithelium                                                                                                        |
|     Hyperplasia                                                                                                                 |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                                                                             |
|-----------        |                                                                                                             |
|OVERALL (a)        |        6/100 (6%)       0/100 (0%)       5/100 (5%)                                                         |
|POLY-3 ADJUSTED (b)|        6.2%             0.0%             5.2%                                                               |
|TERMINAL (d)       |        6/94 (6%)        0/87 (0%)        5/92 (5%)                                                          |
|FIRST INCIDENCE    |        90 (T)           ---              90 (T)                                                             |
|-------------------|                                                                                                             |
|STATISTICAL TESTS  |                                                                                                             |
|-------------------|                                                                                                             |
|LIFE TABLE         |        P=0.446          P=0.024N*        P=0.515N                                                           |
|POLY 3             |        P=0.448          P=0.020N*        P=0.506N                                                           |
|POLY 1.5           |        P=0.446          P=0.019N*        P=0.503N                                                           |
|POLY 6             |        P=0.451          P=0.020N*        P=0.510N                                                           |
|LOGISTIC REGRESSION|        P=0.446          (e)              P=0.515N                                                           |
|COCH-ARM / FISHERS |        P=0.443          P=0.014N*        P=0.500N                                                           |
|=================================================================================================================================|
|                   |                                                                                                             |
|                   |                                                    Females                                                  |
|Dose               |        20 DMBA          50 HZ/1G         50 HZ/5G                                                           |
|                   |        CONTROL           20 DMBA          20 DMBA                                                           |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung: Alveolus                                                                                                                   |
|     Infiltration Cellular Histiocyte                                                                                            |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                                                                             |
|-----------        |                                                                                                             |
|OVERALL (a)        |        7/100 (7%)       2/100 (2%)       15/100 (15%)                                                       |
|POLY-3 ADJUSTED (b)|        7.2%             2.1%             15.5%                                                              |
|TERMINAL (d)       |        7/94 (7%)        2/87 (2%)        15/92 (16%)                                                        |
|FIRST INCIDENCE    |        90 (T)           90 (T)           90 (T)                                                             |
|-------------------|                                                                                                             |
|STATISTICAL TESTS  |                                                                                                             |
|-------------------|                                                                                                             |
|LIFE TABLE         |        P=0.004 **       P=0.106N         P=0.051                                                            |
|POLY 3             |        P=0.004 **       P=0.092N         P=0.053                                                            |
|POLY 1.5           |        P=0.003 **       P=0.089N         P=0.055                                                            |
|POLY 6             |        P=0.004 **       P=0.096N         P=0.052                                                            |
|LOGISTIC REGRESSION|        P=0.004 **       P=0.106N         P=0.051                                                            |
|COCH-ARM / FISHERS |        P=0.004 **       P=0.085N         P=0.056                                                            |
|=================================================================================================================================|
       Date:  01/07/98                           EXPERIMENT: 89029 TEST: 12                                            Page 5

Statistical Analysis of Non-neoplastic Lesions in       Rats(SPRAGUE-DAWLEY)  -     MAGNETIC FIELDS + DMBA INITIATION PROMOTIO
                                         Terminal Sacrifice at 12 weeks 
|=================================================================================================================================|
|                   |                                                                                                             |
|                   |                                                    Females                                                  |
|Dose               |        20 DMBA          50 HZ/1G         50 HZ/5G                                                           |
|                   |        CONTROL           20 DMBA          20 DMBA                                                           |
|=================================================================================================================================|
|                                                                                                                                 |
|Mammary Gland                                                                                                                    |
|     Hyperplasia                                                                                                                 |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                                                                             |
|-----------        |                                                                                                             |
|OVERALL (a)        |        4/100 (4%)       0/100 (0%)       3/100 (3%)                                                         |
|POLY-3 ADJUSTED (b)|        4.1%             0.0%             3.1%                                                               |
|TERMINAL (d)       |        4/94 (4%)        0/87 (0%)        3/92 (3%)                                                          |
|FIRST INCIDENCE    |        90 (T)           ---              90 (T)                                                             |
|-------------------|                                                                                                             |
|STATISTICAL TESTS  |                                                                                                             |
|-------------------|                                                                                                             |
|LIFE TABLE         |        P=0.572          P=0.076N         P=0.512N                                                           |
|POLY 3             |        P=0.574          P=0.067N         P=0.505N                                                           |
|POLY 1.5           |        P=0.573          P=0.066N         P=0.503N                                                           |
|POLY 6             |        P=0.576          P=0.069N         P=0.508N                                                           |
|LOGISTIC REGRESSION|        P=0.572          (e)              P=0.512N                                                           |
|COCH-ARM / FISHERS |        P=0.570          P=0.061N         P=0.500N                                                           |
|=================================================================================================================================|

(a)  Number of tumor-bearing animals /  number of animals examined at site.
(b)  Poly-3 adjusted lifetime tumor incidence.
(d)  Observed incidence at terminal kill.
(f)  Beneath the control incidence are the P-values associated with the trend 
test.  Beneath the dosed group incidence are the P-values corresponding to 
pairwise comparisons between the controls and that dosed group.  The life
table analysis regards tumors in animals dying prior to  terminal kill as
 being (directly or indirectly) the cause of death.
Logistic regression is an alternative
 method for analyzing the incidence of non-fatal tumors. The Cochran-Armitage
and Fishers exact tests compare directly the overall incidence rates
For all tests a negative trend is indicated by N
(e)  Value of Statistic cannot be computed.
(I)  Interim sacrifice
(T)  Terminal sacrifice
#   Tumor rates based on number of animals necropsied.
*   To the right of any statistical result, indicates significance at (P<=0.05).
**  To the right of any statistical result, indicates significance at (P<=0.01).