National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/2244

TR 393 Mouse Pathology Tables


                                     INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)
                                                          SODIUM FLUORIDE


NTP Experiment-Test: 05131-02                                                                                     Report: PEIRPT03
Study Type: CHRONIC                                                                                               Date: 09/17/94
Route: DOSED WATER                                                                                                Time: 08:28:01




       Facility:  Battelle Columbus Laboratory

       Chemical CAS #:  7681-49-4

       Lock Date:  None

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All
































a  Number of animals examined microscopically at site and number of animals with lesion

                                                             Page   1

NTP Experiment-Test: 05131-02         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                          SODIUM FLUORIDE                                      Date: 09/17/94  
Route: DOSED WATER                                                                                                Time: 08:28:01  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                         0 PPM       SPECIAL       25 PPM       100 PPM      175 PPM                     
                                                                CONTROL                                                             
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY                                                                                                                 
  Animals Initially In Study                         100           50           70           70           99                        
  Scheduled Sacrifice                                 20                        18           20           19                        
  Early Deaths                                                                                                                      
    Natural Death                                     13            7            5            7           16                        
    Moribund Sacrifice                                14            6            9            9           12                        
  Survivors                                                                                                                         
    Terminal Sacrifice                                53            8           38           32           51                        
    Natural Death                                                                             2            1                        
  Special Control                                                  29                                                               
  Animals Examined Microscopically                   100           42           62           63           99                        
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Esophagus                                          (99)         (42)         (60)         (60)         (99)                      
      Inflammation, Chronic Active                                  1 (2%)                                                          
   Gallbladder                                        (99)         (40)         (59)         (58)         (98)                      
      Cyst                                                                       1 (2%)                                             
      Mineralization                                   1 (1%)                                                                       
      Artery, Necrosis, Fibrinoid                                                             1 (2%)                                
   Intestine Small, Duodenum                          (100)        (42)         (60)         (58)         (98)                      
      Inflammation, Chronic Active                                                            2 (3%)       2 (2%)                   
   Intestine Small, Ileum                             (98)         (42)         (60)         (59)         (97)                      
      Lymphoid Tissue, Hyperplasia                                  1 (2%)                                                          
   Intestine Small, Jejunum                           (100)        (42)         (60)         (59)         (97)                      
      Inflammation, Chronic Active                     1 (1%)                                                                       
      Lymphoid Tissue, Hyperplasia                     3 (3%)                                                                       
   Liver                                              (100)        (42)         (60)         (60)         (99)                      
      Angiectasis                                                                             1 (2%)                                
      Basophilic Focus                                 2 (2%)       6 (14%)      1 (2%)       3 (5%)                                
      Clear Cell Focus                                 3 (3%)       2 (5%)       2 (3%)       2 (3%)       3 (3%)                   
      Eosinophilic Focus                              23 (23%)      4 (10%)     22 (37%)     11 (18%)     12 (12%)                  
      Hematopoietic Cell Proliferation                 6 (6%)       2 (5%)       3 (5%)       3 (5%)       6 (6%)                   
      Hepatodiaphragmatic Nodule                                    1 (2%)       1 (2%)                    1 (1%)                   
      Inflammation, Chronic Active                     2 (2%)                                 1 (2%)       3 (3%)                   
      Leukocytosis                                                  2 (5%)                                                          
      Mineralization                                                                          1 (2%)                                
      Necrosis, Coagulative                            7 (7%)       4 (10%)      4 (7%)       9 (15%)      3 (3%)                   
      Pigmentation, Hemosiderin                                     1 (2%)                    1 (2%)                                
      Vacuolization Cytoplasmic                        6 (6%)       5 (12%)      4 (7%)       7 (12%)      6 (6%)                   
      Artery, Necrosis, Fibrinoid                                                             1 (2%)                                
      Bile Duct, Cyst                                  1 (1%)       1 (2%)       1 (2%)                                             
   Mesentery                                          (28)         (6)          (16)         (18)         (28)                      
      Hemorrhage                                       1 (4%)                                                                       
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    2                                                              
NTP Experiment-Test: 05131-02         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                          SODIUM FLUORIDE                                      Date: 09/17/94  
Route: DOSED WATER                                                                                                Time: 08:28:01  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                         0 PPM       SPECIAL       25 PPM       100 PPM      175 PPM                     
                                                                CONTROL                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - CONT                                                                                                            
      Inflammation, Chronic Active                                  1 (17%)                                1 (4%)                   
      Inflammation, Necrotizing                       24 (86%)      3 (50%)     13 (81%)     14 (78%)     15 (54%)                  
      Mineralization                                  11 (39%)                   7 (44%)      5 (28%)      4 (14%)                  
      Artery, Necrosis, Fibrinoid                                                             1 (6%)                                
   Pancreas                                           (100)        (42)         (60)         (59)         (98)                      
      Infiltration Cellular, Lymphocyte                                          1 (2%)                                             
      Inflammation, Chronic Active                     1 (1%)       1 (2%)       1 (2%)                                             
      Acinus, Atrophy                                  3 (3%)                                 4 (7%)       2 (2%)                   
      Acinus, Hyperplasia                                                                                  2 (2%)                   
      Duct, Ectasia                                                                           1 (2%)       3 (3%)                   
   Salivary Glands                                    (99)         (41)         (60)         (60)         (99)                      
      Duct, Ectasia                                                                                        1 (1%)                   
   Stomach, Forestomach                               (100)        (42)         (60)         (59)         (98)                      
      Acanthosis                                       5 (5%)                    6 (10%)      5 (8%)       3 (3%)                   
      Diverticulum                                     1 (1%)       1 (2%)       1 (2%)                                             
      Inflammation, Chronic Active                     3 (3%)       1 (2%)       5 (8%)       3 (5%)       5 (5%)                   
   Stomach, Glandular                                 (100)        (42)         (60)         (59)         (98)                      
      Cyst                                                                       1 (2%)                                             
      Erosion                                          1 (1%)       2 (5%)                                 3 (3%)                   
      Hyperplasia                                      2 (2%)                                                                       
      Inflammation, Chronic Active                                               1 (2%)                                             
      Mineralization                                                             1 (2%)                    4 (4%)                   
   Tooth                                              (90)         (42)         (60)         (60)         (89)                      
      Dentine, Incisor, Dysplasia                     34 (38%)      1 (2%)      21 (35%)     23 (38%)     24 (27%)                  
      Dentine, Incisor, Necrosis                                                              2 (3%)       2 (2%)                   
      Incisor, Lower, Dysplasia                                     1 (2%)                                                          
      Incisor, Ameloblast, Atrophy                     1 (1%)                                 1 (2%)       3 (3%)                   
      Incisor, Ameloblast, Degeneration                                                       1 (2%)                                
      Peridontal Tissue, Incisor, Inflammation,                                                                                     
           Suppurative                                                                        1 (2%)                                
      Peridontal Tissue, Incisor, Necrosis                                                                 1 (1%)                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Blood Vessel                                       (1)                                    (1)                                    
      Artery, Necrosis, Fibrinoid                                                             1 (100%)                              
   Heart                                              (99)         (41)         (60)         (60)         (99)                      
      Cardiomyopathy, Chronic                          1 (1%)                    1 (2%)       1 (2%)                                
      Inflammation, Chronic Active                     1 (1%)                                                                       
      Artery, Necrosis, Fibrinoid                                                             1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    3                                                              
NTP Experiment-Test: 05131-02         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                          SODIUM FLUORIDE                                      Date: 09/17/94  
Route: DOSED WATER                                                                                                Time: 08:28:01  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                         0 PPM       SPECIAL       25 PPM       100 PPM      175 PPM                     
                                                                CONTROL                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Gland, Cortex                              (99)         (42)         (60)         (60)         (99)                      
      Accessory Adrenal Cortical Nodule                3 (3%)                                              1 (1%)                   
      Cyst                                                                                    1 (2%)       1 (1%)                   
      Cytoplasmic Alteration                                                                               1 (1%)                   
      Degeneration, Fatty                              2 (2%)                    3 (5%)       1 (2%)                                
      Fibrosis                                                                   1 (2%)                                             
      Hematopoietic Cell Proliferation                 1 (1%)                                 1 (2%)       2 (2%)                   
      Hyperplasia                                      3 (3%)       1 (2%)       1 (2%)                    2 (2%)                   
      Hypertrophy                                      6 (6%)                    1 (2%)       4 (7%)                                
      Capsule, Spindle Cell, Hyperplasia               1 (1%)                    1 (2%)       3 (5%)       1 (1%)                   
   Adrenal Gland, Medulla                             (96)         (42)         (60)         (60)         (98)                      
      Hyperplasia                                      1 (1%)                    1 (2%)                                             
      Hypertrophy                                      1 (1%)                                                                       
   Parathyroid Gland                                  (92)         (37)         (59)         (57)         (92)                      
      Cyst                                             1 (1%)                    1 (2%)                    1 (1%)                   
      Hyperplasia                                                   1 (3%)                                                          
   Pituitary Gland                                    (100)        (41)         (59)         (61)         (97)                      
      Craniopharyngeal Duct, Pars Distalis, Cyst                                              1 (2%)                                
      Craniopharyngeal Duct, Pars Distalis,                                                                                         
          Inflammation, Suppurative                                                           1 (2%)                                
      Pars Distalis, Cyst                              5 (5%)       1 (2%)                    4 (7%)       2 (2%)                   
      Pars Distalis, Hyperplasia                      16 (16%)      1 (2%)      20 (34%)     16 (26%)     25 (26%)                  
   Thyroid Gland                                      (100)        (42)         (60)         (60)         (99)                      
      Inflammation, Chronic Active                     1 (1%)       1 (2%)       1 (2%)       2 (3%)       2 (2%)                   
      Ultimobranchial Cyst                             1 (1%)                    1 (2%)       1 (2%)       3 (3%)                   
      Follicle, Cyst                                                                                       1 (1%)                   
      Follicular Cell, Hyperplasia                    30 (30%)      8 (19%)     27 (45%)     24 (40%)     33 (33%)                  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Clitoral Gland                                     (2)                       (2)                       (3)                       
      Pigmentation, Melanin                            1 (50%)                                                                      
      Duct, Dilatation                                 1 (50%)                   1 (50%)                                            
   Ovary                                              (98)         (41)         (57)         (58)         (98)                      
      Atrophy                                         63 (64%)     14 (34%)     38 (67%)     39 (67%)     59 (60%)                  
      Cyst                                            24 (24%)     12 (29%)     25 (44%)     16 (28%)     25 (26%)                  
      Inflammation, Chronic Active                                  1 (2%)                                                          
      Mineralization                                   1 (1%)                    1 (2%)                                             
      Necrosis, Coagulative                                         1 (2%)                                 1 (1%)                   
      Thrombosis                                                                 2 (4%)                    1 (1%)                   
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    4                                                              
NTP Experiment-Test: 05131-02         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                          SODIUM FLUORIDE                                      Date: 09/17/94  
Route: DOSED WATER                                                                                                Time: 08:28:01  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                         0 PPM       SPECIAL       25 PPM       100 PPM      175 PPM                     
                                                                CONTROL                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
GENITAL SYSTEM - CONT                                                                                                               
   Uterus                                             (100)        (42)         (60)         (60)         (99)                      
      Angiectasis                                      1 (1%)                                              1 (1%)                   
      Dilatation                                      13 (13%)     11 (26%)     14 (23%)      6 (10%)     14 (14%)                  
      Hyperplasia, Cystic, Glandular                  81 (81%)     34 (81%)     54 (90%)     56 (93%)     81 (82%)                  
      Inflammation, Necrotizing                        1 (1%)                                                                       
      Inflammation, Suppurative                                                                            1 (1%)                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (100)        (42)         (62)         (61)         (99)                      
      Femoral, Hyperplasia, Mast Cell                  1 (1%)                                                                       
      Femoral, Myelofibrosis                          34 (34%)      5 (12%)     34 (55%)     34 (56%)     53 (54%)                  
      Humerus, Myelofibrosis                          28 (28%)      7 (17%)     27 (44%)     26 (43%)     47 (47%)                  
      Mandible, Myelofibrosis                          1 (1%)                                              1 (1%)                   
      Maxilla, Hyperplasia, Mast Cell                  1 (1%)                                                                       
      Maxilla, Myelofibrosis                          19 (19%)      3 (7%)      20 (32%)     21 (34%)     44 (44%)                  
      Thoracic, Vertebra, Myelofibrosis               61 (61%)     20 (48%)     50 (81%)     49 (80%)     74 (75%)                  
      Tibia, Myelofibrosis                            14 (14%)      3 (7%)      16 (26%)      9 (15%)     26 (26%)                  
   Lymph Node                                         (100)        (42)         (60)         (60)         (99)                      
      Mediastinal, Hyperplasia, Plasma Cell                         1 (2%)                                                          
      Renal, Sinus, Ectasia                                                                   1 (2%)                                
   Lymph Node, Mandibular                             (96)         (42)         (60)         (56)         (98)                      
      Hyperplasia, Lymphoid                                                                                2 (2%)                   
   Lymph Node, Mesenteric                             (99)         (40)         (57)         (57)         (94)                      
      Hyperplasia, Plasma Cell                         1 (1%)                                                                       
      Sinus, Ectasia                                   1 (1%)                    1 (2%)       1 (2%)                                
   Spleen                                             (99)         (42)         (60)         (59)         (99)                      
      Depletion Lymphoid                                                                      1 (2%)                                
      Fibrosis                                                                                1 (2%)                                
      Hematopoietic Cell Proliferation                20 (20%)     10 (24%)     15 (25%)     15 (25%)     20 (20%)                  
      Hemorrhage                                                                                           1 (1%)                   
      Hyperplasia, Lymphoid                           10 (10%)      3 (7%)       7 (12%)      7 (12%)     11 (11%)                  
   Thymus                                             (96)         (42)         (56)         (55)         (92)                      
      Cyst                                             4 (4%)       3 (7%)       6 (11%)      4 (7%)       6 (7%)                   
      Ectopic Parathyroid Gland                                     1 (2%)                                                          
      Hyperplasia, Lymphoid                                                      3 (5%)                    2 (2%)                   
      Artery, Necrosis, Fibrinoid                                                             1 (2%)                                
      Thymocyte, Necrosis                                           1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (98)         (41)         (59)         (60)         (99)                      
      Hyperplasia, Cystic                              8 (8%)       7 (17%)      8 (14%)      8 (13%)      7 (7%)                   
      Inflammation, Chronic Active                                  1 (2%)                                                          
   Skin                                               (100)        (42)         (60)         (60)         (99)                      
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    5                                                              
NTP Experiment-Test: 05131-02         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                          SODIUM FLUORIDE                                      Date: 09/17/94  
Route: DOSED WATER                                                                                                Time: 08:28:01  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                         0 PPM       SPECIAL       25 PPM       100 PPM      175 PPM                     
                                                                CONTROL                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
INTEGUMENTARY SYSTEM - CONT                                                                                                         
      Acanthosis                                       1 (1%)                                                                       
      Alopecia                                                                                1 (2%)                                
      Cyst Epithelial Inclusion                                                  1 (2%)                                             
      Hyperkeratosis                                   1 (1%)                                                                       
      Inflammation, Chronic Active                     2 (2%)                    2 (3%)       1 (2%)       2 (2%)                   
      Mineralization                                   1 (1%)                                                                       
      Artery, Necrosis, Fibrinoid                                                             1 (2%)                                
      Hair Follicle, Cyst                              1 (1%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (90)         (42)         (60)         (60)         (89)                      
      Femur, Osteosclerosis                                                                                1 (1%)                   
      Humerus, Osteosclerosis                          2 (2%)                                              2 (2%)                   
      Humerus, Periosteum, Proliferation, Focal                                  1 (2%)                                             
      Intervertebral Disc, Degeneration               14 (16%)      2 (5%)      13 (22%)      8 (13%)     16 (18%)                  
      Mandible, Inflammation, Suppurative                           1 (2%)                                                          
      Periosteum, Tibia, Proliferation, Focal          1 (1%)                                                                       
      Periosteum, Maxilla, Proliferation, Focal                                  1 (2%)                                             
      Rib, Cartilage, Degeneration                                               1 (2%)       1 (2%)       1 (1%)                   
      Rib, Cartilage, Necrosis                         1 (1%)                                                                       
      Thoracic, Vertebra, Osteosclerosis               1 (1%)                    1 (2%)                    3 (3%)                   
      Tibia, Osteosclerosis                                         1 (2%)                                 2 (2%)                   
   Skeletal Muscle                                    (12)         (1)                       (1)          (11)                      
      Inflammation, Chronic Active                                  1 (100%)                                                        
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (100)        (42)         (60)         (60)         (99)                      
      Compression                                      3 (3%)                    2 (3%)       2 (3%)       1 (1%)                   
      Hydrocephalus                                    3 (3%)                    1 (2%)       1 (2%)                                
      Inflammation, Chronic Active                                                                         1 (1%)                   
      Artery, Necrosis, Fibrinoid                                                             1 (2%)                                
      Neuron, Necrosis                                                           1 (2%)                                             
      White Matter, Degeneration                                                 1 (2%)                                             
   Peripheral Nerve                                   (1)                       (1)          (3)          (1)                       
      Sciatic, Degeneration                                                      1 (100%)     1 (33%)                               
      Spinal, Degeneration                                                                    1 (33%)                               
   Spinal Cord                                        (1)                       (1)          (3)          (1)                       
      White Matter, Degeneration                                                              1 (33%)      1 (100%)                 
____________________________________________________________________________________________________________________________________
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    6                                                              
NTP Experiment-Test: 05131-02         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                          SODIUM FLUORIDE                                      Date: 09/17/94  
Route: DOSED WATER                                                                                                Time: 08:28:01  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                         0 PPM       SPECIAL       25 PPM       100 PPM      175 PPM                     
                                                                CONTROL                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (100)        (42)         (60)         (60)         (99)                      
      Inflammation, Chronic Active                     3 (3%)       1 (2%)       4 (7%)       1 (2%)       3 (3%)                   
      Leukocytosis                                                  2 (5%)                    1 (2%)                                
      Mineralization                                                             1 (2%)       1 (2%)                                
      Pigmentation, Hemosiderin                                                  1 (2%)                                             
      Alveolar Epithelium, Hyperplasia                 2 (2%)       2 (5%)       3 (5%)       1 (2%)                                
   Nose                                               (100)        (42)         (60)         (60)         (99)                      
      Inflammation, Chronic Active                                  1 (2%)                    1 (2%)                                
      Nasolacrimal Duct, Inflammation, Suppurative     1 (1%)                    1 (2%)       3 (5%)       1 (1%)                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Ear                                                                          (1)          (1)          (1)                       
      Middle Ear, Inflammation, Suppurative                                                   1 (100%)                              
   Eye                                                (1)          (1)          (2)          (3)          (1)                       
      Inflammation, Chronic Active                     1 (100%)                                                                     
      Phthisis Bulbi                                                             1 (50%)                                            
      Cornea, Inflammation, Chronic Active                                                    2 (67%)                               
      Lens, Cataract                                                             1 (50%)                                            
      Retina, Atrophy                                                            1 (50%)                                            
   Harderian Gland                                    (66)         (36)         (48)         (45)         (52)                      
      Hyperplasia                                                   1 (3%)       2 (4%)                                             
      Inflammation, Chronic Active                                  1 (3%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (100)        (42)         (60)         (60)         (99)                      
      Atypia Cellular                                                                                      1 (1%)                   
      Cyst                                                          1 (2%)       2 (3%)                    1 (1%)                   
      Hydronephrosis                                   1 (1%)                                 1 (2%)                                
      Hypertrophy                                      1 (1%)                                                                       
      Metaplasia, Osseous                                                        1 (2%)       1 (2%)       1 (1%)                   
      Mineralization                                  63 (63%)     22 (52%)     34 (57%)     26 (43%)     28 (28%)                  
      Necrosis, Coagulative                                         1 (2%)                                                          
      Nephropathy, Chronic                            52 (52%)     12 (29%)     43 (72%)     29 (48%)     26 (26%)                  
      Artery, Necrosis, Fibrinoid                                                             1 (2%)                                
      Perivascular, Hyperplasia, Lymphoid                                                                  2 (2%)                   
      Renal Tubule, Necrosis                                                                               3 (3%)                   
   Urinary Bladder                                    (100)        (42)         (60)         (60)         (97)                      
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    7                                                              
NTP Experiment-Test: 05131-02         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                          SODIUM FLUORIDE                                      Date: 09/17/94  
Route: DOSED WATER                                                                                                Time: 08:28:01  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                         0 PPM       SPECIAL       25 PPM       100 PPM      175 PPM                     
                                                                CONTROL                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
URINARY SYSTEM - CONT                                                                                                               
      Inflammation, Necrotizing                        1 (1%)                                                                       
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    8                                                              
NTP Experiment-Test: 05131-02         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                          SODIUM FLUORIDE                                      Date: 09/17/94  
Route: DOSED WATER                                                                                                Time: 08:28:01  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                           0 PPM       SPECIAL       25 PPM       100 PPM      175 PPM                     
                                                                CONTROL                                                             
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY                                                                                                                 
  Animals Initially In Study                          99           50           70           70          100                        
  Scheduled Sacrifice                                 20                        20           19           20                        
  Early Deaths                                                                                                                      
    Natural Death                                     16            3            8            6           11                        
    Moribund Sacrifice                                 5            9            3            8            4                        
  Survivors                                                                                                                         
    Natural Death                                      2                                                                            
    Terminal Sacrifice                                56           18           39           37           65                        
  Special Control                                                  20                                                               
  Animals Examined Microscopically                    99           32           60           62          100                        
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Gallbladder                                        (96)         (32)         (58)         (59)         (100)                     
      Cyst                                                          1 (3%)                                                          
   Intestine Small, Duodenum                          (99)         (32)         (60)         (59)         (100)                     
      Erosion                                                                    1 (2%)                                             
      Intussusception                                                                         1 (2%)                                
   Intestine Small, Jejunum                           (99)         (32)         (60)         (59)         (100)                     
      Lymphoid Tissue, Hyperplasia                                               2 (3%)       1 (2%)                                
      Peyer's Patch, Hyperplasia, Lymphoid                                       2 (3%)                                             
   Liver                                              (99)         (32)         (60)         (61)         (100)                     
      Basophilic Focus                                 9 (9%)       1 (3%)       6 (10%)      2 (3%)       5 (5%)                   
      Clear Cell Focus                                36 (36%)      7 (22%)     27 (45%)     31 (51%)     48 (48%)                  
      Clear Cell Focus, Multiple                                    1 (3%)                                                          
      Cytologic Alterations                            1 (1%)                    1 (2%)                                             
      Eosinophilic Focus                               9 (9%)       2 (6%)       9 (15%)     12 (20%)      6 (6%)                   
      Fibrosis                                                                   1 (2%)                                             
      Hyperplasia                                      1 (1%)                    2 (3%)                                             
      Inflammation, Chronic Active                     2 (2%)       2 (6%)       7 (12%)      1 (2%)                                
      Mineralization                                                             1 (2%)                    1 (1%)                   
      Mixed Cell Focus                                                                        1 (2%)       2 (2%)                   
      Necrosis, Coagulative                            4 (4%)       3 (9%)       5 (8%)       4 (7%)       4 (4%)                   
      Vacuolization Cytoplasmic                       24 (24%)     13 (41%)     18 (30%)     17 (28%)     27 (27%)                  
      Bile Duct, Cyst                                                                         1 (2%)                                
      Bile Duct, Hyperplasia                                                     1 (2%)                                             
   Mesentery                                          (10)         (2)          (5)          (8)          (4)                       
      Inflammation, Chronic Active                                                            1 (13%)      1 (25%)                  
      Inflammation, Necrotizing                        8 (80%)      1 (50%)      4 (80%)      6 (75%)      2 (50%)                  
      Mineralization                                   1 (10%)                   2 (40%)      2 (25%)      1 (25%)                  
   Pancreas                                           (98)         (32)         (60)         (60)         (100)                     
      Inflammation, Chronic Active                     1 (1%)                                                                       
      Acinus, Amyloid Deposition                                                              1 (2%)                                
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    9                                                              
NTP Experiment-Test: 05131-02         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                          SODIUM FLUORIDE                                      Date: 09/17/94  
Route: DOSED WATER                                                                                                Time: 08:28:01  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                           0 PPM       SPECIAL       25 PPM       100 PPM      175 PPM                     
                                                                CONTROL                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - CONT                                                                                                            
      Acinus, Atrophy                                  3 (3%)                    1 (2%)       1 (2%)       1 (1%)                   
      Acinus, Inflammation, Chronic Active             1 (1%)                    1 (2%)                                             
      Acinus, Necrosis, Coagulative                                              1 (2%)                                             
   Stomach, Forestomach                               (99)         (32)         (60)         (60)         (99)                      
      Acanthosis                                       2 (2%)                    1 (2%)       1 (2%)                                
      Cyst Epithelial Inclusion                                                               1 (2%)       1 (1%)                   
      Diverticulum                                                  1 (3%)                    1 (2%)       2 (2%)                   
      Inflammation, Chronic Active                     1 (1%)                    1 (2%)       1 (2%)                                
      Mineralization                                                1 (3%)                                                          
   Stomach, Glandular                                 (99)         (32)         (60)         (60)         (100)                     
      Erosion                                          1 (1%)                    1 (2%)       1 (2%)       2 (2%)                   
      Inflammation, Chronic Active                     1 (1%)                                                                       
      Mineralization                                   2 (2%)                    2 (3%)       1 (2%)       4 (4%)                   
      Perivascular, Inflammation, Chronic Active                                              1 (2%)                                
   Tooth                                              (90)         (32)         (60)         (60)         (90)                      
      Dentine, Incisor, Concretion                                                                         1 (1%)                   
      Dentine, Incisor, Degeneration                                             1 (2%)                                             
      Dentine, Incisor, Dysplasia                     69 (77%)     17 (53%)     52 (87%)     48 (80%)     82 (91%)                  
      Dentine, Incisor, Necrosis                       2 (2%)                                              2 (2%)                   
      Gingiva, Incisor, Inflammation, Suppurative                                1 (2%)                                             
      Incisor, Lower, Dysplasia                        8 (9%)       6 (19%)     14 (23%)     17 (28%)     14 (16%)                  
      Incisor, Ameloblast, Atrophy                    42 (47%)     15 (47%)     29 (48%)     27 (45%)     50 (56%)                  
      Peridontal Tissue, Incisor, Inflammation,                                                                                     
           Suppurative                                                           1 (2%)                    1 (1%)                   
      Pulp, Incisor, Inflammation, Suppurative                                                1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Blood Vessel                                       (3)                       (1)          (1)          (2)                       
      Inflammation, Chronic Active                                               1 (100%)                                           
      Aorta, Inflammation, Chronic Active                                                     1 (100%)     1 (50%)                  
      Mesenteric Artery, Inflammation, Chronic                                                                                      
          Active                                       1 (33%)                                1 (100%)                              
      Mesenteric Artery, Necrosis, Fibrinoid           1 (33%)                                                                      
      Renal Artery, Inflammation, Chronic Active       1 (33%)                                                                      
      Thoracic, Artery, Inflammation, Chronic                                                                                       
          Active                                                                              1 (100%)                              
   Heart                                              (99)         (32)         (60)         (59)         (100)                     
      Cardiomyopathy, Chronic                          2 (2%)                    1 (2%)                    1 (1%)                   
      Mineralization                                                2 (6%)                                                          
      Perivascular, Inflammation, Chronic Active                                              1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   10                                                              
NTP Experiment-Test: 05131-02         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                          SODIUM FLUORIDE                                      Date: 09/17/94  
Route: DOSED WATER                                                                                                Time: 08:28:01  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                           0 PPM       SPECIAL       25 PPM       100 PPM      175 PPM                     
                                                                CONTROL                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Gland, Cortex                              (99)         (32)         (60)         (60)         (100)                     
      Accessory Adrenal Cortical Nodule                2 (2%)                    1 (2%)                    1 (1%)                   
      Atrophy                                                                    1 (2%)                                             
      Cyst                                             2 (2%)                    2 (3%)       1 (2%)                                
      Hyperplasia                                      3 (3%)       1 (3%)       1 (2%)       4 (7%)       7 (7%)                   
      Hypertrophy                                     34 (34%)      7 (22%)     16 (27%)     23 (38%)     32 (32%)                  
      Bilateral, Capsule, Spindle Cell, Hyperplasia    1 (1%)       1 (3%)                    1 (2%)       2 (2%)                   
      Capsule, Spindle Cell, Hyperplasia              11 (11%)      1 (3%)       8 (13%)     10 (17%)     13 (13%)                  
   Adrenal Gland, Medulla                             (96)         (32)         (60)         (60)         (99)                      
      Hyperplasia                                                                1 (2%)       1 (2%)       1 (1%)                   
   Islets, Pancreatic                                 (98)         (32)         (60)         (60)         (100)                     
      Hyperplasia                                     13 (13%)      7 (22%)      7 (12%)     17 (28%)     15 (15%)                  
   Parathyroid Gland                                  (88)         (30)         (55)         (55)         (97)                      
      Cyst                                             2 (2%)                    1 (2%)                                             
   Pituitary Gland                                    (91)         (32)         (59)         (57)         (93)                      
      Pars Distalis, Cyst                              2 (2%)                    6 (10%)      5 (9%)       4 (4%)                   
      Pars Distalis, Hyperplasia                       3 (3%)                    1 (2%)       2 (4%)                                
      Pars Intermedia, Cyst                                                      1 (2%)                                             
   Thyroid Gland                                      (99)         (32)         (60)         (60)         (100)                     
      Cyst                                             1 (1%)                                                                       
      Inflammation, Chronic Active                     1 (1%)                    1 (2%)       1 (2%)       1 (1%)                   
      Ultimobranchial Cyst                                          1 (3%)                    2 (3%)       1 (1%)                   
      Follicle, Cyst                                                                                       1 (1%)                   
      Follicular Cell, Hyperplasia                     6 (6%)       1 (3%)      10 (17%)      6 (10%)     12 (12%)                  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Epididymis                                         (99)         (32)         (60)         (60)         (99)                      
      Aspermia                                         1 (1%)                                              2 (2%)                   
      Atrophy                                                                                              2 (2%)                   
      Granuloma Sperm                                  2 (2%)                    2 (3%)                    4 (4%)                   
      Inflammation, Chronic Active                     2 (2%)       1 (3%)                    1 (2%)       1 (1%)                   
      Mineralization                                                             1 (2%)       2 (3%)                                
      Duct, Dilatation                                 1 (1%)                                                                       
   Preputial Gland                                    (57)         (12)         (40)         (33)         (55)                      
      Atrophy                                         48 (84%)     11 (92%)     30 (75%)     27 (82%)     35 (64%)                  
      Inflammation, Chronic Active                    22 (39%)      5 (42%)     18 (45%)     14 (42%)     25 (45%)                  
      Duct, Dilatation                                51 (89%)     11 (92%)     36 (90%)     32 (97%)     47 (85%)                  
   Prostate                                           (99)         (32)         (60)         (60)         (99)                      
      Atrophy                                                                                              2 (2%)                   
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   11                                                              
NTP Experiment-Test: 05131-02         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                          SODIUM FLUORIDE                                      Date: 09/17/94  
Route: DOSED WATER                                                                                                Time: 08:28:01  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                           0 PPM       SPECIAL       25 PPM       100 PPM      175 PPM                     
                                                                CONTROL                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
GENITAL SYSTEM - CONT                                                                                                               
      Inflammation, Chronic Active                     3 (3%)       1 (3%)       1 (2%)                    1 (1%)                   
   Seminal Vesicle                                    (99)         (32)         (60)         (60)         (100)                     
      Cyst                                                                       1 (2%)       1 (2%)                                
      Inflammation, Chronic Active                     2 (2%)       1 (3%)       2 (3%)       1 (2%)       1 (1%)                   
   Testes                                             (98)         (31)         (60)         (60)         (99)                      
      Mineralization                                                1 (3%)                    2 (3%)                                
      Necrosis, Coagulative                                                                   1 (2%)                                
      Seminiferous Tubule, Atrophy                                                                         2 (2%)                   
      Seminiferous Tubule, Degeneration                                          2 (3%)                    3 (3%)                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (99)         (32)         (60)         (60)         (100)                     
      Femoral, Myelofibrosis                           1 (1%)                    1 (2%)       5 (8%)       2 (2%)                   
      Femoral, Necrosis, Coagulative                                                                       1 (1%)                   
      Humerus, Myelofibrosis                                                     1 (2%)       2 (3%)                                
      Maxilla, Myelofibrosis                           1 (1%)                    2 (3%)                                             
      Thoracic, Vertebra, Myelofibrosis                                                                    1 (1%)                   
      Tibia, Myelofibrosis                                                                    1 (2%)                                
   Lymph Node                                         (99)         (32)         (60)         (60)         (100)                     
      Inguinal, Hyperplasia, Plasma Cell                                                                   1 (1%)                   
      Renal, Sinus, Ectasia                                                      1 (2%)                                             
   Lymph Node, Mandibular                             (98)         (31)         (60)         (59)         (100)                     
      Hyperplasia, Plasma Cell                                                                2 (3%)                                
   Lymph Node, Mesenteric                             (94)         (31)         (59)         (56)         (92)                      
      Hematopoietic Cell Proliferation                                                        1 (2%)                                
      Hyperplasia, Lymphoid                                                      4 (7%)                                             
      Inflammation, Chronic Active                                                                         1 (1%)                   
      Thrombosis                                                                 1 (2%)                                             
   Spleen                                             (99)         (32)         (60)         (60)         (100)                     
      Atrophy                                                                                 1 (2%)       2 (2%)                   
      Hematopoietic Cell Proliferation                 8 (8%)       3 (9%)      10 (17%)      7 (12%)      9 (9%)                   
      Hyperplasia, Lymphoid                            1 (1%)                                                                       
   Thymus                                             (87)         (28)         (52)         (45)         (85)                      
      Atrophy                                                                    2 (4%)                                             
      Cyst                                            11 (13%)      3 (11%)     13 (25%)      6 (13%)     11 (13%)                  
      Hyperplasia, Lymphoid                                                                                1 (1%)                   
      Inflammation, Chronic Active                     1 (1%)                                                                       
      Thymocyte, Necrosis                                                                                  1 (1%)                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   12                                                              
NTP Experiment-Test: 05131-02         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                          SODIUM FLUORIDE                                      Date: 09/17/94  
Route: DOSED WATER                                                                                                Time: 08:28:01  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                           0 PPM       SPECIAL       25 PPM       100 PPM      175 PPM                     
                                                                CONTROL                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Skin                                               (99)         (31)         (60)         (60)         (100)                     
      Autolysis                                        1 (1%)                                                                       
      Inflammation, Chronic Active                     2 (2%)                                              1 (1%)                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (89)         (32)         (60)         (60)         (90)                      
      Calvarium, Hyperostosis                                                                              1 (1%)                   
      Calvarium, Osteosclerosis, Focal                                                        1 (2%)                                
      Humerus, Osteosclerosis                                                    1 (2%)                                             
      Humerus, Joint, Cartilage, Hyperplasia, Focal                                                        1 (1%)                   
      Intervertebral Disc, Degeneration                6 (7%)       2 (6%)      10 (17%)      6 (10%)      8 (9%)                   
      Joint, Cartilage, Tibia, Degeneration                                                                2 (2%)                   
      Rib, Cartilage, Degeneration                                                            1 (2%)       2 (2%)                   
      Thoracic, Vertebra, Osteosclerosis               2 (2%)       1 (3%)                                 3 (3%)                   
      Tibia, Fracture Healed                                        1 (3%)                                                          
      Tibia, Osteosclerosis                                                                                1 (1%)                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (99)         (32)         (60)         (60)         (100)                     
      Infiltration Cellular, Lymphocyte                                          1 (2%)                                             
      Infiltration Cellular, Histiocyte                                                                    1 (1%)                   
      Inflammation, Chronic Active                     4 (4%)                    2 (3%)       2 (3%)       4 (4%)                   
      Leukocytosis                                     1 (1%)       1 (3%)                                 1 (1%)                   
      Mineralization                                                                                       1 (1%)                   
      Pigmentation, Hemosiderin                        1 (1%)                                                                       
      Thrombosis                                                                                           1 (1%)                   
      Alveolar Epithelium, Hyperplasia                 4 (4%)                    4 (7%)       4 (7%)       3 (3%)                   
      Bronchus, Dilatation                                                       1 (2%)                                             
      Mediastinum, Mineralization                      1 (1%)                                                                       
   Nose                                               (99)         (32)         (60)         (60)         (100)                     
      Inflammation, Chronic Active                     1 (1%)       1 (3%)                                 2 (2%)                   
      Nasolacrimal Duct, Hyperplasia, Squamous         1 (1%)                                                                       
      Nasolacrimal Duct, Inflammation, Suppurative     7 (7%)                    5 (8%)       2 (3%)       2 (2%)                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   13                                                              
NTP Experiment-Test: 05131-02         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                          SODIUM FLUORIDE                                      Date: 09/17/94  
Route: DOSED WATER                                                                                                Time: 08:28:01  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                           0 PPM       SPECIAL       25 PPM       100 PPM      175 PPM                     
                                                                CONTROL                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Ear                                                (1)          (1)                       (2)          (1)                       
      Middle Ear, Inflammation, Suppurative                                                   1 (50%)                               
   Eye                                                (5)                                                                           
      Phthisis Bulbi                                   1 (20%)                                                                      
      Cornea, Inflammation, Chronic Active             2 (40%)                                                                      
      Cornea, Mineralization                           1 (20%)                                                                      
      Lens, Cataract                                   2 (40%)                                                                      
      Retina, Atrophy                                  1 (20%)                                                                      
   Harderian Gland                                    (85)         (27)         (49)         (52)         (75)                      
      Hyperplasia                                                                1 (2%)                                             
      Inflammation, Chronic Active                                                            2 (4%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (99)         (32)         (60)         (60)         (100)                     
      Cyst                                            17 (17%)      4 (13%)     10 (17%)     13 (22%)     11 (11%)                  
      Hydronephrosis                                                2 (6%)       1 (2%)                                             
      Inflammation, Chronic Active                     2 (2%)       1 (3%)       2 (3%)                    1 (1%)                   
      Inflammation, Necrotizing                                                               1 (2%)                                
      Metaplasia, Osseous                                                                     1 (2%)                                
      Mineralization                                  75 (76%)     28 (88%)     47 (78%)     44 (73%)     73 (73%)                  
      Necrosis, Coagulative                            1 (1%)                                              1 (1%)                   
      Nephropathy, Chronic                            76 (77%)     27 (84%)     56 (93%)     53 (88%)     78 (78%)                  
      Artery, Necrosis, Fibrinoid                      1 (1%)                                                                       
      Pelvis, Bacterium                                                                                    1 (1%)                   
      Renal Tubule, Epithelium, Hyperplasia                                                   1 (2%)                                
   Urinary Bladder                                    (98)         (32)         (60)         (59)         (100)                     
      Calculus Gross Observation                                                 1 (2%)                                             
      Calculus Micro Observation Only                                                         1 (2%)                                
      Inflammation, Chronic Active                     2 (2%)                                 1 (2%)                                
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   14                                                              
                             ------------------------------------------------------------                                           
                             ----------              END OF REPORT             ----------                                           
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NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.