National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/20999

TDMS Study 91005-07 Pathology Tables

NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05
Route: GAVAGE                                                                                                     Time: 10:12:13

                                                         FINAL #1 RATS




       Facility:  Southern Research Institute

       Chemical CAS #:  7220-79-3

       Lock Date:  09/05/03

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All




































                                                              Page   1


NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 5| 7| 6| 7| 7| 6| 7| 6| 6| 7| 6| 6| 7| 3| 7| 6| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 3| 9| 3| 4| 3| 3| 5| 3| 5| 7| 3| 4| 3| 3| 4| 3| 9| 3| 3| 3| 3| 3| 1|             
                                           | 4| 4| 4| 1| 4| 8| 6| 6| 2| 6| 2| 4| 4| 0| 8| 4| 4| 4| 8| 4| 6| 6| 6| 6| 0|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Basophilic Focus                     | X  X  X     X     X     X     2  X  X  X     X     2     X  X  X     X  X|             
      Clear Cell Focus                     |    X                                         X                           |             
      Degeneration, Cystic                 |                                                                1         |             
      Eosinophilic Focus                   |                                                       X                  |             
      Hepatodiaphragmatic Nodule           |                X                                               2  3      |             
      Inflammation, Chronic                |    1  2                 2                                                |             
      Necrosis, Focal                      |                                                                          |             
      Bile Duct, Hyperplasia               |    2                                      2                       2      |             
      Centrilobular, Necrosis              |                                                                          |             
      Hepatocyte, Vacuolization Cytoplasmic|          2     2           3              2                              |             
                                           |__________________________________________________________________________|             
   Mesentery                               |    +        +  +        +        +     +        +     +  +        +     +|             
      Fat, Necrosis                        |    3        4  2        3        3     3        2     3  2        3     3|             
                                           |__________________________________________________________________________|             
   Oral Mucosa                             |          +                                                               |             
      Pharyngeal, Cyst                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              | 2  2  1           1  1  3     1  1        1  2     1  2  1     1  2  1   |             
      Acinus, Hyperplasia, Focal           |                                                       2                  |             
                                           |__________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page   2                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 5| 7| 6| 7| 7| 6| 7| 6| 6| 7| 6| 6| 7| 3| 7| 6| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 3| 9| 3| 4| 3| 3| 5| 3| 5| 7| 3| 4| 3| 3| 4| 3| 9| 3| 3| 3| 3| 3| 1|             
                                           | 4| 4| 4| 1| 4| 8| 6| 6| 2| 6| 2| 4| 4| 0| 8| 4| 4| 4| 8| 4| 6| 6| 6| 6| 0|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM - cont                  |                                                                          |             
                                           |__________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Edema                                |                            2                                             |             
      Inflammation, Chronic Active         |                                                                          |             
      Ulcer                                |                            3              2                              |             
      Epithelium, Hyperplasia              |                                                                          |             
                                           |__________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Edema                                |                            2                                             |             
      Erosion                              |          2                                3                              |             
      Ulcer                                |                            2                                             |             
                                           |__________________________________________________________________________|             
   Tongue                                  |                                              +                           |             
      Hyperplasia                          |                                              2                           |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Blood Vessel                            |                +                                                         |             
                                           |__________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       | 1  1  1  1  1  1  2  1  1  1     1  1        2  2        1  2        1   |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Accessory Adrenal Cortical Nodule    |       1           1           1           1                    1         |             
      Degeneration, Fatty                  |                   1  1     3  1     2        1                 2  1      |             
      Hyperplasia, Focal                   |                   1                 2        2                           |             
      Hypertrophy, Focal                   |                      2        1     3                          2  2      |             
      Necrosis                             |                                                       2                  |             
                                           |__________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          | 1  1  1                 1  1        1     1  1                           |             
      Metaplasia, Hepatocyte               |       2                                                                  |             
                                           |__________________________________________________________________________|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page   3                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 5| 7| 6| 7| 7| 6| 7| 6| 6| 7| 6| 6| 7| 3| 7| 6| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 3| 9| 3| 4| 3| 3| 5| 3| 5| 7| 3| 4| 3| 3| 4| 3| 9| 3| 3| 3| 3| 3| 1|             
                                           | 4| 4| 4| 1| 4| 8| 6| 6| 2| 6| 2| 4| 4| 0| 8| 4| 4| 4| 8| 4| 6| 6| 6| 6| 0|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ENDOCRINE SYSTEM - cont                   |                                                                          |             
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Pars Distalis, Angiectasis           |       2     2  2  2  2     2              2  2        2  2  2        2   |             
      Pars Distalis, Cyst                  |    2     2  2                 2              2     2     3  3  2  2     2|             
      Pars Distalis, Hyperplasia, Focal    |                                     2                                    |             
      Pars Intermedia, Cyst                |                                                                          |             
                                           |__________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Ultimobranchial Cyst                 |                                     1                                    |             
      C-Cell, Hyperplasia                  |          2                    3                                2  2     1|             
      Follicle, Cyst                       | 2                                2                                       |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
    None                                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |       2                                      3                           |             
      Inflammation, Chronic                |                                                                          |             
                                           |__________________________________________________________________________|             
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 | 2  1  2                                                        3     1   |             
                                           |__________________________________________________________________________|             
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                   X      |             
      Hemorrhage                           |                                                                          |             
      Hydrometra                           |                               3                                          |             
      Cervix, Cyst                         |    X                                                                     |             
      Endometrium, Hyperplasia, Cystic     |                                     2                                    |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                  4     2        1                       2|             
                                           |__________________________________________________________________________|             
   Lymph Node                              |          +     +           +              +           +           +      |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page   4                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 5| 7| 6| 7| 7| 6| 7| 6| 6| 7| 6| 6| 7| 3| 7| 6| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 3| 9| 3| 4| 3| 3| 5| 3| 5| 7| 3| 4| 3| 3| 4| 3| 9| 3| 3| 3| 3| 3| 1|             
                                           | 4| 4| 4| 1| 4| 8| 6| 6| 2| 6| 2| 4| 4| 0| 8| 4| 4| 4| 8| 4| 6| 6| 6| 6| 0|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
      Mediastinal, Congestion              |                                                                          |             
      Mediastinal, Hyperplasia, Lymphoid   |                                                                          |             
      Pancreatic, Hyperplasia, Lymphoid    |                                                                          |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mandibular                  | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|             
                                           |__________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Fibrosis                             |                                                                          |             
      Hematopoietic Cell Proliferation     |                                  4                                       |             
      Necrosis                             |          3                                                               |             
      Capsule, Fibrosis                    | 1                                                  1                 1  1|             
      Lymphoid Follicle, Hyperplasia       |                                                                          |             
                                           |__________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Atypical                |                                                    1                     |             
      Hyperplasia                          | 2           4                                               3     2     2|             
      Duct, Dilatation                     | 3  2  2  2  2  3  3  2     4              2  2     2  3  3  4  2  3  2   |             
      Duct, Hyperplasia                    |                                  2  2                                    |             
                                           |__________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cranium, Osteopetrosis               |          2                                                               |             
                                           |__________________________________________________________________________|             
   Skeletal Muscle                         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Compression                          |                                                       2                  |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page   5                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 5| 7| 6| 7| 7| 6| 7| 6| 6| 7| 6| 6| 7| 3| 7| 6| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 3| 9| 3| 4| 3| 3| 5| 3| 5| 7| 3| 4| 3| 3| 4| 3| 9| 3| 3| 3| 3| 3| 1|             
                                           | 4| 4| 4| 1| 4| 8| 6| 6| 2| 6| 2| 4| 4| 0| 8| 4| 4| 4| 8| 4| 6| 6| 6| 6| 0|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 NERVOUS SYSTEM - cont                     |                                                                          |             
      Hemorrhage                           |                2                                                         |             
                                           |__________________________________________________________________________|             
   Peripheral Nerve                        |                                              +                           |             
                                           |__________________________________________________________________________|             
   Spinal Cord                             |                                              +                           |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                                             2            |             
      Infiltration Cellular, Histiocyte    |    1  1  1  1     1  1  1     1  1  1  1  1  1  1  1  1  1  1  1  1     1|             
      Inflammation, Chronic                |       1     1     1  1  1     1     1  1     1     1     1  1  1        1|             
      Alveolar Epithelium, Hyperplasia     |                                                                          |             
                                           |__________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         | 2                                                                        |             
      Inflammation, Chronic                | 2              2     2  2                                                |             
                                           |__________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cataract                             |                                              2                           |             
      Cornea, Inflammation, Chronic        |                                                                          |             
      Retina, Degeneration                 |                                              3                           |             
                                           |__________________________________________________________________________|             
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic                |                                        1                                 |             
      Pigmentation                         |          2                                            2                  |             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Infarct                              |                2                                                         |             
      Nephropathy                          |    1     1           1           1                          1        1   |             
                                           |__________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________              
                                                                                                                                    
                                                                                                                                    
                                                             Page   6                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 5| 7| 7| 7| 7| 7| 7| 6| 5| 7| 7| 7| 7| 7| 6| 7| 6| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 1| 3| 3| 3| 3| 3| 3| 5| 6| 3| 3| 3| 3| 3| 6| 3| 4| 3| 3| 3| 3| 3| 3|            |
                                           | 4| 4| 6| 4| 4| 7| 7| 7| 7| 2| 3| 7| 7| 7| 7| 4| 8| 4| 8| 4| 3| 3| 3| 3| 3|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Basophilic Focus                     | 2  X     X  X     X  X  X        X  X  X  X  X  X  X  X  X  X  X  X     X|     37  2.0|
      Clear Cell Focus                     |          X  X        X     X              X        X                     |      8     |
      Degeneration, Cystic                 |                                                                          |      1  1.0|
      Eosinophilic Focus                   |          2                          X                                    |      3  2.0|
      Hepatodiaphragmatic Nodule           |                2                 2                 2        X            |      7  2.2|
      Inflammation, Chronic                |          1              4                                         2      |      6  2.0|
      Necrosis, Focal                      |       2                                                                  |      1  2.0|
      Bile Duct, Hyperplasia               | 2              4     2                                               3   |      7  2.4|
      Centrilobular, Necrosis              |                2                                            1            |      2  1.5|
      Hepatocyte, Vacuolization Cytoplasmic|                                                                          |      4  2.3|
                                           |__________________________________________________________________________|____________|
   Mesentery                               | +           +                       +              +        +           +|  17        |
      Fat, Necrosis                        | 2           3                       3              3        3           3|     17  2.8|
                                           |__________________________________________________________________________|____________|
   Oral Mucosa                             |          +                                                               |   2        |
      Pharyngeal, Cyst                     |          3                                                               |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page   7                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 5| 7| 7| 7| 7| 7| 7| 6| 5| 7| 7| 7| 7| 7| 6| 7| 6| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 1| 3| 3| 3| 3| 3| 3| 5| 6| 3| 3| 3| 3| 3| 6| 3| 4| 3| 3| 3| 3| 3| 3|            |
                                           | 4| 4| 6| 4| 4| 7| 7| 7| 7| 2| 3| 7| 7| 7| 7| 4| 8| 4| 8| 4| 3| 3| 3| 3| 3|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
      Atrophy                              | 1           1  2        1                    4     1  2     1  1     1   |     26  1.5|
      Acinus, Hyperplasia, Focal           |          2                                            1                  |      3  1.7|
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Edema                                |                               3                                          |      2  2.5|
      Inflammation, Chronic Active         |                               1                                          |      1  1.0|
      Ulcer                                |       2                                                              2   |      4  2.3|
      Epithelium, Hyperplasia              |       2                       2                                          |      2  2.0|
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Edema                                |                               2                                          |      2  2.0|
      Erosion                              |                                                                          |      2  2.5|
      Ulcer                                |       2                                                                  |      2  2.0|
                                           |__________________________________________________________________________|____________|
   Tongue                                  |                                                                          |   1        |
      Hyperplasia                          |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Blood Vessel                            |                                                                          |   1        |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cardiomyopathy                       | 2  1     2  1     1  1  2  1  1  2  2        2  1  1  1  1  1  2  1      |     36  1.3|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Accessory Adrenal Cortical Nodule    |                4                    1        1     1                     |      9  1.3|
      Degeneration, Fatty                  |    1        1        1                                                   |     11  1.4|
      Hyperplasia, Focal                   |                                              1                       1   |      5  1.4|
      Hypertrophy, Focal                   |    2        1     2  2  1           4     1                              |     12  1.9|
      Necrosis                             |                               4                                          |      2  3.0|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page   8                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 5| 7| 7| 7| 7| 7| 7| 6| 5| 7| 7| 7| 7| 7| 6| 7| 6| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 1| 3| 3| 3| 3| 3| 3| 5| 6| 3| 3| 3| 3| 3| 6| 3| 4| 3| 3| 3| 3| 3| 3|            |
                                           | 4| 4| 6| 4| 4| 7| 7| 7| 7| 2| 3| 7| 7| 7| 7| 4| 8| 4| 8| 4| 3| 3| 3| 3| 3|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ENDOCRINE SYSTEM - cont                   |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |                2                                                         |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Hyperplasia                          |    1                                1     1        1              1      |     13  1.0|
      Metaplasia, Hepatocyte               |                                              1                           |      2  1.5|
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | +  +  +  +  +  M  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +|  47        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Pars Distalis, Angiectasis           | 2  2     3  3  2  2  1           2  2     2  2     2  2        2  2     2|     28  2.0|
      Pars Distalis, Cyst                  |    2           2        2  4     2  2        2        2     2        1   |     21  2.1|
      Pars Distalis, Hyperplasia, Focal    |                         2     2        2              2                  |      5  2.0|
      Pars Intermedia, Cyst                |                   2                                                      |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Ultimobranchial Cyst                 |                2                                                         |      2  1.5|
      C-Cell, Hyperplasia                  |                      2     2                             2  2  3         |     10  2.1|
      Follicle, Cyst                       |                                                                          |      2  2.0|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 |                      2                             2                     |      4  2.3|
      Inflammation, Chronic                |                2                                                         |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 |                      2  1                    1     4           2        2|     11  1.9|
                                           |__________________________________________________________________________|____________|
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page   9                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 5| 7| 7| 7| 7| 7| 7| 6| 5| 7| 7| 7| 7| 7| 6| 7| 6| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 1| 3| 3| 3| 3| 3| 3| 5| 6| 3| 3| 3| 3| 3| 6| 3| 4| 3| 3| 3| 3| 3| 3|            |
                                           | 4| 4| 6| 4| 4| 7| 7| 7| 7| 2| 3| 7| 7| 7| 7| 4| 8| 4| 8| 4| 3| 3| 3| 3| 3|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM - cont                     |                                                                          |            |
      Cyst                                 |                                                                          |      1     |
      Hemorrhage                           |                                                                   2      |      1  2.0|
      Hydrometra                           |                                                                          |      1  3.0|
      Cervix, Cyst                         |                                                                          |      1     |
      Endometrium, Hyperplasia, Cystic     |                3                          2           3                  |      4  2.5|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |       2                       3  2                          3            |      8  2.4|
                                           |__________________________________________________________________________|____________|
   Lymph Node                              |          +        +  +     +     +     +                    +        +  +|  15        |
      Mediastinal, Congestion              |                                        3                                 |      1  3.0|
      Mediastinal, Hyperplasia, Lymphoid   |          2        2  2     2                                            2|      5  2.0|
      Pancreatic, Hyperplasia, Lymphoid    |                                                             2            |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|            |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Fibrosis                             |                2                                                     2   |      2  2.0|
      Hematopoietic Cell Proliferation     |                               4                             4            |      3  4.0|
      Necrosis                             |                                                                          |      1  3.0|
      Capsule, Fibrosis                    |          1                          1                 1  1               |      8  1.0|
      Lymphoid Follicle, Hyperplasia       |                                                             3            |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia, Atypical                |    1                                                                 2   |      3  1.3|
      Hyperplasia                          |             2     2           3  2     2  2  3  2  2     2  2     2  2   |     18  2.3|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  10                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 5| 7| 7| 7| 7| 7| 7| 6| 5| 7| 7| 7| 7| 7| 6| 7| 6| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 1| 3| 3| 3| 3| 3| 3| 5| 6| 3| 3| 3| 3| 3| 6| 3| 4| 3| 3| 3| 3| 3| 3|            |
                                           | 4| 4| 6| 4| 4| 7| 7| 7| 7| 2| 3| 7| 7| 7| 7| 4| 8| 4| 8| 4| 3| 3| 3| 3| 3|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 INTEGUMENTARY SYSTEM - cont               |                                                                          |            |
      Duct, Dilatation                     | 3  2     2     2     2  2  4  4  2  4     2                    2        3|     31  2.6|
      Duct, Hyperplasia                    |                                                                          |      2  2.0|
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cranium, Osteopetrosis               |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Skeletal Muscle                         |                                                       +                  |   1        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Compression                          |          3                 2                                             |      3  2.3|
      Hemorrhage                           |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Peripheral Nerve                        |                               +                       +                  |   3        |
                                           |__________________________________________________________________________|____________|
   Spinal Cord                             |                               +                       +                  |   3        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Congestion                           |    1        1  1                                      1                  |      5  1.2|
      Infiltration Cellular, Histiocyte    | 1  1     1  1  1  1  1  1  1     1     1  1  1     1  1  1  1  1  1     1|     41  1.0|
      Inflammation, Chronic                | 1  1        1  1     1  1  1        1  1  1        1  1        1  1     1|     29  1.0|
      Alveolar Epithelium, Hyperplasia     |    3                                                                     |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Foreign Body                         |                      1  2              2                                 |      4  1.8|
      Inflammation, Chronic                |                      1  2              2                 2               |      8  1.9|
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  11                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 5| 7| 7| 7| 7| 7| 7| 6| 5| 7| 7| 7| 7| 7| 6| 7| 6| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 1| 3| 3| 3| 3| 3| 3| 5| 6| 3| 3| 3| 3| 3| 6| 3| 4| 3| 3| 3| 3| 3| 3|            |
                                           | 4| 4| 6| 4| 4| 7| 7| 7| 7| 2| 3| 7| 7| 7| 7| 4| 8| 4| 8| 4| 3| 3| 3| 3| 3|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 RESPIRATORY SYSTEM - cont                 |                                                                          |            |
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cataract                             |                                                                          |      1  2.0|
      Cornea, Inflammation, Chronic        |                               2                                          |      1  2.0|
      Retina, Degeneration                 |                               2                                          |      2  2.5|
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation, Chronic                |                   2        2                                             |      3  1.7|
      Pigmentation                         |                                                                          |      2  2.0|
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Infarct                              |                                                                          |      1  2.0|
      Nephropathy                          | 1           1              1     1           1     1  1        1         |     14  1.0|
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  12                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 3| 7| 7| 6| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 8| 4| 0| 3| 3| 3| 7| 3| 3| 3| 3| 3| 3| 2| 3| 9| 0| 3| 4| 3| 3| 3| 3| 3|             
                                           | 6| 7| 4| 2| 6| 7| 7| 7| 7| 7| 6| 6| 6| 6| 8| 7| 5| 2| 7| 1| 4| 4| 4| 4| 4|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    5 MG/KG                                | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Basophilic Focus                     | X           X  1     X     1  X  X  X  X  X  X        X  X  X  X  X  X   |             
      Clear Cell Focus                     |                   X                                                      |             
      Degeneration, Cystic                 |                                                                          |             
      Eosinophilic Focus                   |                                              X                           |             
      Hepatodiaphragmatic Nodule           |             X                                                  2         |             
      Inflammation, Chronic                |                         2        2        1                              |             
      Bile Duct, Hyperplasia               |                                           1        2                     |             
      Hepatocyte, Vacuolization Cytoplasmic|    1     2        2                             2                        |             
                                           |__________________________________________________________________________|             
   Mesentery                               |             +           +  +           +  +                              |             
      Accessory Spleen                     |                                                                          |             
      Fat, Necrosis                        |             2           3  3           3  2                              |             
                                           |__________________________________________________________________________|             
   Pancreas                                | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              | 2        2                 1  1  1  1              2  1              1   |             
      Inflammation                         | 1                                                                        |             
      Acinus, Hyperplasia, Focal           |                               2                                          |             
                                           |__________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Edema                                |    2                                                     3               |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  13                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 3| 7| 7| 6| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 8| 4| 0| 3| 3| 3| 7| 3| 3| 3| 3| 3| 3| 2| 3| 9| 0| 3| 4| 3| 3| 3| 3| 3|             
                                           | 6| 7| 4| 2| 6| 7| 7| 7| 7| 7| 6| 6| 6| 6| 8| 7| 5| 2| 7| 1| 4| 4| 4| 4| 4|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    5 MG/KG                                | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM - cont                  |                                                                          |             
      Ulcer                                |    3                                                     4               |             
      Epithelium, Hyperplasia              |                                                          2               |             
                                           |__________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Edema                                |    2                                                                     |             
      Erosion                              |                                                 3                        |             
      Ulcer                                |    3                                                                     |             
                                           |__________________________________________________________________________|             
   Tongue                                  |                                                                          |             
      Cyst                                 |                                                                          |             
      Inflammation                         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       | 1  1        1  1  1  1  1  1  1     1  1  1  2     1  2  1  2  1  2  2  2|             
      Thrombosis                           |          2                                                               |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Accessory Adrenal Cortical Nodule    | 1        2                                                  1           2|             
      Degeneration, Fatty                  |                   2     2     2  1  2                 2                 3|             
      Hyperplasia, Focal                   |                            1  2                    1                     |             
      Hypertrophy, Focal                   |                   2     2     3  2  2                 2                 3|             
      Necrosis                             |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                        1                                1|             
                                           |__________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                   1  1                                1        1  1      |             
                                           |__________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Pars Distalis, Angiectasis           |       2     1  2     2              2  2  2  1        2        2  2  2  1|             
      Pars Distalis, Cyst                  |    2     2  1  1           2  3  2     2        2        1  1     1      |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  14                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 3| 7| 7| 6| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 8| 4| 0| 3| 3| 3| 7| 3| 3| 3| 3| 3| 3| 2| 3| 9| 0| 3| 4| 3| 3| 3| 3| 3|             
                                           | 6| 7| 4| 2| 6| 7| 7| 7| 7| 7| 6| 6| 6| 6| 8| 7| 5| 2| 7| 1| 4| 4| 4| 4| 4|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    5 MG/KG                                | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ENDOCRINE SYSTEM - cont                   |                                                                          |             
      Pars Distalis, Hyperplasia, Focal    |             1              2  2  2  2                                    |             
                                           |__________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      C-Cell, Hyperplasia                  |             1        1     1           2                    2        2   |             
      Follicle, Cyst                       |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
    None                                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Clitoral Gland                          | +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                   3     2                       2        2               |             
      Inflammation, Chronic                |             2  1                             2                    2      |             
                                           |__________________________________________________________________________|             
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |             1                                            1  3            |             
                                           |__________________________________________________________________________|             
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                X              X                                          |             
      Hydrometra                           | 1           1           1                                                |             
      Endometrium, Hyperplasia, Cystic     |                                                    1                     |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |    4              3              2              2     2  3               |             
                                           |__________________________________________________________________________|             
   Lymph Node                              |          +     +  +                             +     +                  |             
      Mediastinal, Hyperplasia, Lymphoid   |          2     2  3                             2     2                  |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mandibular                  | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|             
                                           |__________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Lymphoid                |             2                                                            |             
                                           |__________________________________________________________________________|             
   Spleen                                  | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hematopoietic Cell Proliferation     |    3              2                             3                        |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  15                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 3| 7| 7| 6| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 8| 4| 0| 3| 3| 3| 7| 3| 3| 3| 3| 3| 3| 2| 3| 9| 0| 3| 4| 3| 3| 3| 3| 3|             
                                           | 6| 7| 4| 2| 6| 7| 7| 7| 7| 7| 6| 6| 6| 6| 8| 7| 5| 2| 7| 1| 4| 4| 4| 4| 4|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    5 MG/KG                                | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
      Capsule, Fibrosis                    |                1  1  1        1     1  1  1           1           1  1   |             
                                           |__________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Galactocele                          |                                                                X         |             
      Hyperplasia, Atypical                | 2                                                           2            |             
      Hyperplasia                          |                2                          2        2  2        2  2  3  2|             
      Duct, Dilatation                     |    2  2  2  3  2     3  2  2  2     2  2  4  4  2     3     2     2      |             
                                           |__________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic                |                         1                                                |             
      Inflammation, Chronic Active         | 4                                                                        |             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Compression                          |                         3                 2                    2         |             
      Hemorrhage                           |                                                                          |             
                                           |__________________________________________________________________________|             
   Peripheral Nerve                        |                   +              +                                       |             
                                           |__________________________________________________________________________|             
   Spinal Cord                             |                   +              +                                       |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |       1                                                                  |             
      Infiltration Cellular, Histiocyte    | 1        1  1  1  2  1        1  1  1  1  1  1  1     1  1     1     1  1|             
      Inflammation, Chronic                |    1        1  1     1  1     1  1  1  1  1  1     1  1  1     1     1  1|             
      Alveolar Epithelium, Hyperplasia     |                                                                          |             
                                           |__________________________________________________________________________|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  16                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 3| 7| 7| 6| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 8| 4| 0| 3| 3| 3| 7| 3| 3| 3| 3| 3| 3| 2| 3| 9| 0| 3| 4| 3| 3| 3| 3| 3|             
                                           | 6| 7| 4| 2| 6| 7| 7| 7| 7| 7| 6| 6| 6| 6| 8| 7| 5| 2| 7| 1| 4| 4| 4| 4| 4|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    5 MG/KG                                | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 RESPIRATORY SYSTEM - cont                 |                                                                          |             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |          1                                                               |             
      Inflammation, Chronic                |          1                                1              1               |             
                                           |__________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cataract                             |                                  3                                       |             
      Inflammation, Chronic                |                   4                                                      |             
      Retina, Degeneration                 |                                  4                                       |             
                                           |__________________________________________________________________________|             
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic                |                      1                                            1      |             
      Pigmentation                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Zymbal's Gland                          |                   +                                                      |             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Nephropathy                          | 1  1     1     1                          2        1  1  1        1     1|             
                                           |__________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  17                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 5| 7| 6| 7| 5| 7| 7| 6| 7| 7| 7| 5| 6| 7| 7| 7| 7| 6| 7| 1| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 6| 3| 4| 3| 7| 3| 3| 9| 3| 3| 3| 8| 2| 0| 3| 3| 3| 6| 3| 3| 1| 3| 3| 3|            |
                                           | 3| 8| 3| 8| 3| 6| 4| 4| 4| 4| 4| 4| 5| 1| 2| 3| 3| 3| 3| 3| 1| 0| 6| 6| 6|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|      A     |
    5 MG/KG                                | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  48        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  48        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  48        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  48        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  48        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
      Basophilic Focus                     | X           X  X  X  X     X  X  X        X  X  X  X     X     X  X  X  X|     34  1.0|
      Clear Cell Focus                     |                      X           X           X           X               |      5     |
      Degeneration, Cystic                 |                                                          2               |      1  2.0|
      Eosinophilic Focus                   |                                                                         X|      2     |
      Hepatodiaphragmatic Nodule           |       2                                                  2               |      4  2.0|
      Inflammation, Chronic                |                                                                   2      |      4  1.8|
      Bile Duct, Hyperplasia               |          2                       2              2                        |      5  1.8|
      Hepatocyte, Vacuolization Cytoplasmic|                                                                          |      4  1.8|
                                           |__________________________________________________________________________|____________|
   Mesentery                               | +     +     +           +     +  +     +     +           +               |  14        |
      Accessory Spleen                     |                         2                                                |      1  2.0|
      Fat, Necrosis                        | 2     3     3           2     2  3     3     3           3               |     14  2.6|
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  48        |
      Atrophy                              |       1           2        2  1     2  1  1  1  1  1     2               |     20  1.4|
      Inflammation                         |                                                                          |      1  1.0|
      Acinus, Hyperplasia, Focal           |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  18                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 5| 7| 6| 7| 5| 7| 7| 6| 7| 7| 7| 5| 6| 7| 7| 7| 7| 6| 7| 1| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 6| 3| 4| 3| 7| 3| 3| 9| 3| 3| 3| 8| 2| 0| 3| 3| 3| 6| 3| 3| 1| 3| 3| 3|            |
                                           | 3| 8| 3| 8| 3| 6| 4| 4| 4| 4| 4| 4| 5| 1| 2| 3| 3| 3| 3| 3| 1| 0| 6| 6| 6|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|      A     |
    5 MG/KG                                | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
      Edema                                |                         2                                                |      3  2.3|
      Ulcer                                |                         2                                                |      3  3.0|
      Epithelium, Hyperplasia              |                         2                                                |      2  2.0|
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
      Edema                                |                                                                          |      1  2.0|
      Erosion                              |                                     2                                    |      2  2.5|
      Ulcer                                |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Tongue                                  |          +                                                               |   1        |
      Cyst                                 |          2                                                               |      1  2.0|
      Inflammation                         |          3                                                               |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
      Cardiomyopathy                       | 1     1  1  1  1  2  1     1  1  1  1  1     1  2  1     1     2  1  1  1|     41  1.2|
      Thrombosis                           |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
      Accessory Adrenal Cortical Nodule    | 1                          1              1                              |      7  1.3|
      Degeneration, Fatty                  |       2     2     2  2        1                                      1   |     13  1.8|
      Hyperplasia, Focal                   |       2           2                                            2     1  2|      8  1.6|
      Hypertrophy, Focal                   |       2     2        2        1                                          |     11  2.1|
      Necrosis                             |                                     2                                    |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
      Hyperplasia                          |                                                                          |      2  1.0|
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  19                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 5| 7| 6| 7| 5| 7| 7| 6| 7| 7| 7| 5| 6| 7| 7| 7| 7| 6| 7| 1| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 6| 3| 4| 3| 7| 3| 3| 9| 3| 3| 3| 8| 2| 0| 3| 3| 3| 6| 3| 3| 1| 3| 3| 3|            |
                                           | 3| 8| 3| 8| 3| 6| 4| 4| 4| 4| 4| 4| 5| 1| 2| 3| 3| 3| 3| 3| 1| 0| 6| 6| 6|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|      A     |
    5 MG/KG                                | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ENDOCRINE SYSTEM - cont                   |                                                                          |            |
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  48        |
      Hyperplasia                          | 1     1  1     1  1        1  1  1     1     1                           |     15  1.0|
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
      Pars Distalis, Angiectasis           | 2  2        2        2  2  2           2  2  2     2  2  2        2     2|     27  1.9|
      Pars Distalis, Cyst                  |       1  2     2              2  2              1        1               |     19  1.6|
      Pars Distalis, Hyperplasia, Focal    |       1        2                 2                                       |      8  1.8|
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
      C-Cell, Hyperplasia                  |          1  2  1  1                                            1         |     11  1.4|
      Follicle, Cyst                       |                      1                                                   |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  48        |
      Cyst                                 |             3  1                          1                              |      7  2.0|
      Inflammation, Chronic                |                                  1                                       |      5  1.6|
                                           |__________________________________________________________________________|____________|
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
      Cyst                                 |                                                                   2      |      4  1.8|
                                           |__________________________________________________________________________|____________|
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
      Cyst                                 |                                                          X               |      3     |
      Hydrometra                           |                                                                         1|      4  1.0|
      Endometrium, Hyperplasia, Cystic     |                                                                          |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  20                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 5| 7| 6| 7| 5| 7| 7| 6| 7| 7| 7| 5| 6| 7| 7| 7| 7| 6| 7| 1| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 6| 3| 4| 3| 7| 3| 3| 9| 3| 3| 3| 8| 2| 0| 3| 3| 3| 6| 3| 3| 1| 3| 3| 3|            |
                                           | 3| 8| 3| 8| 3| 6| 4| 4| 4| 4| 4| 4| 5| 1| 2| 3| 3| 3| 3| 3| 1| 0| 6| 6| 6|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|      A     |
    5 MG/KG                                | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
      Hyperplasia                          |             2                                                  4         |      8  2.8|
                                           |__________________________________________________________________________|____________|
   Lymph Node                              |          +     +                             +                           |   8        |
      Mediastinal, Hyperplasia, Lymphoid   |                2                             2                           |      7  2.1|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M     M  M  M  M|            |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
      Hyperplasia, Lymphoid                |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  48        |
      Hematopoietic Cell Proliferation     |                                                                2  2      |      5  2.4|
      Capsule, Fibrosis                    |       1              2  2  1     1     1              1                  |     17  1.1|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
      Galactocele                          |                                                                          |      1     |
      Hyperplasia, Atypical                |                                                                      2   |      3  2.0|
      Hyperplasia                          |    3  1  2  2  2     1        3  2           2        2                 2|     19  2.1|
      Duct, Dilatation                     |                   2     3  3           2  4  3  2     3  2        2  4   |     28  2.5|
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
      Inflammation, Chronic                |          2                                                               |      2  1.5|
      Inflammation, Chronic Active         |                                                                          |      1  4.0|
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  21                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 5| 7| 6| 7| 5| 7| 7| 6| 7| 7| 7| 5| 6| 7| 7| 7| 7| 6| 7| 1| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 6| 3| 4| 3| 7| 3| 3| 9| 3| 3| 3| 8| 2| 0| 3| 3| 3| 6| 3| 3| 1| 3| 3| 3|            |
                                           | 3| 8| 3| 8| 3| 6| 4| 4| 4| 4| 4| 4| 5| 1| 2| 3| 3| 3| 3| 3| 1| 0| 6| 6| 6|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|      A     |
    5 MG/KG                                | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 NERVOUS SYSTEM - cont                     |                                                                          |            |
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
      Compression                          | 2                       2                 2           2                 2|      8  2.1|
      Hemorrhage                           |          2                                                               |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Peripheral Nerve                        |    +              +                                                      |   4        |
                                           |__________________________________________________________________________|____________|
   Spinal Cord                             |                   +                                                      |   3        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
      Congestion                           |                                        1                                 |      2  1.0|
      Infiltration Cellular, Histiocyte    | 1  1  1     1  1  1  1     1  1        1  1  1  1  1     1     1  1  1  1|     37  1.0|
      Inflammation, Chronic                | 1  1  1        1  1        1  1  1     1     1  1  1     1        1  1  1|     33  1.0|
      Alveolar Epithelium, Hyperplasia     |    2  2                    2                          2                  |      4  2.0|
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
      Foreign Body                         |                                                    1     1               |      3  1.0|
      Inflammation, Chronic                |                      1                             1     1           1   |      7  1.0|
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
      Cataract                             |                   3                                                      |      2  3.0|
      Inflammation, Chronic                |                4                                                         |      2  4.0|
      Retina, Degeneration                 |                   4                                                      |      2  4.0|
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
      Inflammation, Chronic                |                2                                                         |      3  1.3|
      Pigmentation                         |          2                          2                                    |      2  2.0|
                                           |__________________________________________________________________________|____________|
   Zymbal's Gland                          |                                                                          |   1        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  22                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 5| 7| 6| 7| 5| 7| 7| 6| 7| 7| 7| 5| 6| 7| 7| 7| 7| 6| 7| 1| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 6| 3| 4| 3| 7| 3| 3| 9| 3| 3| 3| 8| 2| 0| 3| 3| 3| 6| 3| 3| 1| 3| 3| 3|            |
                                           | 3| 8| 3| 8| 3| 6| 4| 4| 4| 4| 4| 4| 5| 1| 2| 3| 3| 3| 3| 3| 1| 0| 6| 6| 6|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|      A     |
    5 MG/KG                                | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
      Nephropathy                          |                      1     1  1              1                          2|     15  1.1|
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +|  49        |
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  23                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 7| 7| 7| 7| 5| 5| 7| 5| 7| 4| 7|             
                             DAY ON TEST   | 3| 0| 3| 3| 3| 5| 3| 3| 3| 3| 4| 3| 3| 2| 3| 3| 3| 3| 9| 6| 3| 9| 3| 9| 3|             
                                           | 3| 2| 3| 0| 3| 1| 6| 6| 6| 6| 6| 6| 6| 1| 6| 6| 6| 6| 6| 3| 6| 3| 6| 2| 6|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    25 MG/KG                               | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Perforation                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  +  A  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  A  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  A  +  A  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Basophilic Focus                     | X  X  X  X  X     X  X  X  X  X  X  X     X  X  X  X  X     X            |             
      Clear Cell Focus                     |                      X           X  X                 X                  |             
      Hepatodiaphragmatic Nodule           | 2           2                                               2  X         |             
      Inflammation, Chronic                |                         2                                                |             
      Necrosis, Focal                      |                                                                         1|             
      Bile Duct, Hyperplasia               |                                           1                       2      |             
      Centrilobular, Necrosis              |                                        2                                 |             
                                           |__________________________________________________________________________|             
   Mesentery                               | +                       +                 +  +                           |             
      Fat, Necrosis                        | 2                       3                 3  2                           |             
                                           |__________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |       1  1  1  1  2  1  2     1     1                 1     1     1     1|             
      Acinus, Hyperplasia, Focal           |             2                                                            |             
                                           |__________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Edema                                |                                                       3                  |             
      Ulcer                                |                                                       3                  |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  24                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 7| 7| 7| 7| 5| 5| 7| 5| 7| 4| 7|             
                             DAY ON TEST   | 3| 0| 3| 3| 3| 5| 3| 3| 3| 3| 4| 3| 3| 2| 3| 3| 3| 3| 9| 6| 3| 9| 3| 9| 3|             
                                           | 3| 2| 3| 0| 3| 1| 6| 6| 6| 6| 6| 6| 6| 1| 6| 6| 6| 6| 6| 3| 6| 3| 6| 2| 6|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    25 MG/KG                               | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM - cont                  |                                                                          |             
      Epithelium, Hyperplasia              |                                                                      1   |             
                                           |__________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Edema                                |                                                       2                  |             
      Ulcer                                |                                                       2                  |             
                                           |__________________________________________________________________________|             
   Tongue                                  |                               +                                          |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Blood Vessel                            |                               +                                          |             
                                           |__________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       | 1  1  2  1  1     1  1  1  1  1  2  1     1  1  2  1  1        1        1|             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Accessory Adrenal Cortical Nodule    |                      1                       2                    1      |             
      Degeneration, Fatty                  | 2     2  1        2  2                             2  2     2            |             
      Hyperplasia, Focal                   |                   1     1           1                             1  2   |             
      Hypertrophy, Focal                   | 2     2  1        2  2                             2  2     2            |             
                                           |__________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                                    3                     |             
                                           |__________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          | 2                 1        1  1     1              1           1         |             
                                           |__________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Pars Distalis, Angiectasis           |    3     2     2  2     2        2  2     2     2     2        2  2  2  2|             
      Pars Distalis, Cyst                  |       2     2        2     2        2              1     2  1           2|             
      Pars Distalis, Hyperplasia, Focal    |       2                    2                                             |             
                                           |__________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Ultimobranchial Cyst                 |                                              1                           |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  25                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 7| 7| 7| 7| 5| 5| 7| 5| 7| 4| 7|             
                             DAY ON TEST   | 3| 0| 3| 3| 3| 5| 3| 3| 3| 3| 4| 3| 3| 2| 3| 3| 3| 3| 9| 6| 3| 9| 3| 9| 3|             
                                           | 3| 2| 3| 0| 3| 1| 6| 6| 6| 6| 6| 6| 6| 1| 6| 6| 6| 6| 6| 3| 6| 3| 6| 2| 6|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    25 MG/KG                               | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ENDOCRINE SYSTEM - cont                   |                                                                          |             
      C-Cell, Hyperplasia                  |             3        1              3     1     1  1                     |             
      Follicle, Cyst                       |                                  1                                       |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
    None                                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |             2           2                                                |             
      Inflammation, Chronic                |                                                                      4   |             
                                           |__________________________________________________________________________|             
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                  3              2           1     1      |             
                                           |__________________________________________________________________________|             
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                  X                                       |             
      Hydrometra                           |    1                                                                    2|             
                                           |__________________________________________________________________________|             
   Vagina                                  |                                                                          |             
      Cyst                                 |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                2              3        2        3                    2   |             
                                           |__________________________________________________________________________|             
   Lymph Node                              |                      +        +        +        +                    +  +|             
      Mediastinal, Hyperplasia, Lymphoid   |                      2        2                 2                    2   |             
      Pancreatic, Hyperplasia, Lymphoid    |                                                                          |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mandibular                  | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|             
                                           |__________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Lymphoid                |                                        1                                 |             
                                           |__________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hematopoietic Cell Proliferation     |                                        2        4        2           1   |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  26                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 7| 7| 7| 7| 5| 5| 7| 5| 7| 4| 7|             
                             DAY ON TEST   | 3| 0| 3| 3| 3| 5| 3| 3| 3| 3| 4| 3| 3| 2| 3| 3| 3| 3| 9| 6| 3| 9| 3| 9| 3|             
                                           | 3| 2| 3| 0| 3| 1| 6| 6| 6| 6| 6| 6| 6| 1| 6| 6| 6| 6| 6| 3| 6| 3| 6| 2| 6|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    25 MG/KG                               | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
      Capsule, Fibrosis                    | 1                 1           2     1     1           1           1  1   |             
      Lymphoid Follicle, Atrophy           |                3                                                         |             
                                           |__________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                         X|             
      Galactocele                          |    X                                                                     |             
      Hyperplasia, Atypical                |                                                                          |             
      Hyperplasia                          |             2                    2              3                       2|             
      Duct, Dilatation                     | 2        2     1  2  1  2  2     4  2  2  2  2     2        2  4  3  1   |             
                                           |__________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Compression                          |    3     2              2                                      2  2      |             
      Hemorrhage                           |                                                                          |             
                                           |__________________________________________________________________________|             
   Peripheral Nerve                        |                +                                      +                  |             
                                           |__________________________________________________________________________|             
   Spinal Cord                             |                                                       +                  |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |          1                                                     1         |             
      Infiltration Cellular, Histiocyte    | 1     1  1        1  1  1  1  1  1        1  1  1  1     1  1     1     1|             
      Inflammation, Chronic                |       1           1  1  1  1     1  1        1     1     1  1     1     1|             
      Inflammation, Suppurative            |                3                                                         |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  27                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 7| 7| 7| 7| 5| 5| 7| 5| 7| 4| 7|             
                             DAY ON TEST   | 3| 0| 3| 3| 3| 5| 3| 3| 3| 3| 4| 3| 3| 2| 3| 3| 3| 3| 9| 6| 3| 9| 3| 9| 3|             
                                           | 3| 2| 3| 0| 3| 1| 6| 6| 6| 6| 6| 6| 6| 1| 6| 6| 6| 6| 6| 3| 6| 3| 6| 2| 6|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    25 MG/KG                               | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 RESPIRATORY SYSTEM - cont                 |                                                                          |             
      Necrosis                             |                3                                                         |             
      Alveolar Epithelium, Hyperplasia     |                   2                                                      |             
                                           |__________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |                                                                          |             
      Inflammation, Chronic                |    2           2              3                 2                    2   |             
                                           |__________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cataract                             |                               1                                         1|             
      Anterior Chamber, Inflammation       |                                                                          |             
      Cornea, Inflammation, Chronic        |                               2                                          |             
      Retina, Degeneration                 |                                                                         3|             
                                           |__________________________________________________________________________|             
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic                |                                                       1                  |             
      Pigmentation                         |                                                                      2   |             
                                           |__________________________________________________________________________|             
   Zymbal's Gland                          |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                          |             
      Infarct                              |                         1                    2                           |             
      Nephropathy                          |       1     1     1     1  1     2     1  1  2     1  1                 1|             
                                           |__________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  28                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 5| 5| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 4| 9| 6| 3| 3| 3| 8| 3| 2| 3| 3| 2| 3|            |
                                           | 7| 7| 7| 7| 7| 4| 4| 4| 4| 7| 6| 6| 0| 6| 0| 6| 6| 6| 7| 6| 9| 6| 6| 3| 6|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|      A     |
    25 MG/KG                               | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Perforation                          |                                                             3            |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  A  +  A  +  +  +  +  +  +  +  +  +  +|  47        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  A  +  A  +  +  +  +  +  +  +  +  +  +|  45        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +|  46        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Basophilic Focus                     | 2  X  X  X  X  X  X  X  X     X  1        X  X  X     X  X     X  2  X  X|     38  1.7|
      Clear Cell Focus                     | X                          X     X           X                       X   |      9     |
      Hepatodiaphragmatic Nodule           |       2        2                                            X            |      7  2.0|
      Inflammation, Chronic                |       2     1                                   2                        |      4  1.8|
      Necrosis, Focal                      |                                                                          |      1  1.0|
      Bile Duct, Hyperplasia               |                   1                 2  2           2                     |      6  1.7|
      Centrilobular, Necrosis              |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Mesentery                               |    +                 +     +  +  +     +                       +  +      |  12        |
      Fat, Necrosis                        |                      3     2  4  3     3                       2  2      |     11  2.6|
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  A  +  A  +  +  +  +  +  +  +  +  +  +|  48        |
      Atrophy                              | 2  2  1  1  1     2     1                    1     2  1                  |     23  1.3|
      Acinus, Hyperplasia, Focal           |                2                                                         |      2  2.0|
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation                         |                                                                1         |      1  1.0|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  29                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 5| 5| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 4| 9| 6| 3| 3| 3| 8| 3| 2| 3| 3| 2| 3|            |
                                           | 7| 7| 7| 7| 7| 4| 4| 4| 4| 7| 6| 6| 0| 6| 0| 6| 6| 6| 7| 6| 9| 6| 6| 3| 6|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|      A     |
    25 MG/KG                               | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +|  49        |
      Edema                                |                                                                          |      1  3.0|
      Ulcer                                |                                                                          |      1  3.0|
      Epithelium, Hyperplasia              |                                     2                                    |      2  1.5|
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  A  +  A  +  +  +  +  +  +  +  +  +  +|  48        |
      Edema                                |                                                                          |      1  2.0|
      Ulcer                                |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Tongue                                  |                                                                          |   1        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Blood Vessel                            |                                                                          |   1        |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cardiomyopathy                       | 2  1     1  1  1  2  1        1     1  1     1  1        1  1  1     1  2|     36  1.2|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Accessory Adrenal Cortical Nodule    | 1        1              1                                      2     1   |      8  1.3|
      Degeneration, Fatty                  |    2           2     3                                      1  2         |     13  1.9|
      Hyperplasia, Focal                   |       1                                               2        2         |      8  1.4|
      Hypertrophy, Focal                   |    3           3     4                             3        1            |     13  2.2|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  A  +  A  +  +  +  +  +  +  +  +  +  +|  48        |
      Hyperplasia                          | 1     1  1           1        1  1           1                       1   |     15  1.1|
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +|  49        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  30                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 5| 5| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 4| 9| 6| 3| 3| 3| 8| 3| 2| 3| 3| 2| 3|            |
                                           | 7| 7| 7| 7| 7| 4| 4| 4| 4| 7| 6| 6| 0| 6| 0| 6| 6| 6| 7| 6| 9| 6| 6| 3| 6|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|      A     |
    25 MG/KG                               | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ENDOCRINE SYSTEM - cont                   |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Pars Distalis, Angiectasis           | 2     2  2  2  2     2  2     2  2     2              2  2  2        2  2|     29  2.0|
      Pars Distalis, Cyst                  |          1        2        2  1     2           2                       1|     16  1.7|
      Pars Distalis, Hyperplasia, Focal    |                2                    2           2                 2      |      6  2.0|
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Ultimobranchial Cyst                 |                                                                          |      1  1.0|
      C-Cell, Hyperplasia                  |                                  3                                       |      7  1.9|
      Follicle, Cyst                       |                                                                   1      |      2  1.0|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 |                            1                                            2|      4  1.8|
      Inflammation, Chronic                |             2                                                            |      2  3.0|
                                           |__________________________________________________________________________|____________|
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 |       2                          1     3        3        2               |      9  2.0|
                                           |__________________________________________________________________________|____________|
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 |                                                                      X   |      2     |
      Hydrometra                           |                                                                          |      2  1.5|
                                           |__________________________________________________________________________|____________|
   Vagina                                  |                                                                      +   |   1        |
      Cyst                                 |                                                                      X   |      1     |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |    3  2                    2                          4                  |      9  2.6|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  31                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 5| 5| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 4| 9| 6| 3| 3| 3| 8| 3| 2| 3| 3| 2| 3|            |
                                           | 7| 7| 7| 7| 7| 4| 4| 4| 4| 7| 6| 6| 0| 6| 0| 6| 6| 6| 7| 6| 9| 6| 6| 3| 6|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|      A     |
    25 MG/KG                               | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lymph Node                              | +        +  +  +                                            +            |  11        |
      Mediastinal, Hyperplasia, Lymphoid   | 2        2     2                                            2            |      8  2.0|
      Pancreatic, Hyperplasia, Lymphoid    |             2                                                            |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|            |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia, Lymphoid                |                                                                          |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +|  49        |
      Hematopoietic Cell Proliferation     |                            4     2                    4                  |      7  2.7|
      Capsule, Fibrosis                    |                   1     1     1                 1                        |     12  1.1|
      Lymphoid Follicle, Atrophy           |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 |                                                                   X      |      2     |
      Galactocele                          |                                                                          |      1     |
      Hyperplasia, Atypical                |                                                       2                  |      1  2.0|
      Hyperplasia                          |          2  3        1                 1                       2         |      9  2.0|
      Duct, Dilatation                     | 3     4        3  2     2     4              2  2  3        4  2  2      |     29  2.4|
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation                         |                            4                                             |      1  4.0|
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  32                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 5| 5| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 4| 9| 6| 3| 3| 3| 8| 3| 2| 3| 3| 2| 3|            |
                                           | 7| 7| 7| 7| 7| 4| 4| 4| 4| 7| 6| 6| 0| 6| 0| 6| 6| 6| 7| 6| 9| 6| 6| 3| 6|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|      A     |
    25 MG/KG                               | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 NERVOUS SYSTEM - cont                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Compression                          | 2                                                                        |      6  2.2|
      Hemorrhage                           |                                     4                                    |      1  4.0|
                                           |__________________________________________________________________________|____________|
   Peripheral Nerve                        |                         +                             +                  |   4        |
                                           |__________________________________________________________________________|____________|
   Spinal Cord                             |                         +                             +                  |   3        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Congestion                           |                                        1  1                 1            |      5  1.0|
      Infiltration Cellular, Histiocyte    | 1  1  1  1  1  1  1  1  1  1     1     1  1  1     1  1  1  1  1  1  1  1|     39  1.0|
      Inflammation, Chronic                | 1  2  1        1  1  1  1        1     1  1  1     1     1     1  1     1|     29  1.0|
      Inflammation, Suppurative            |                                                                          |      1  3.0|
      Necrosis                             |                                                                          |      1  3.0|
      Alveolar Epithelium, Hyperplasia     |                         2                       3                        |      3  2.3|
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Foreign Body                         |                                                       1                  |      1  1.0|
      Inflammation, Chronic                |       2     1           1                             1                  |      9  1.8|
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cataract                             |                         3                             4              3   |      5  2.4|
      Anterior Chamber, Inflammation       |                            2                                             |      1  2.0|
      Cornea, Inflammation, Chronic        |                                                                          |      1  2.0|
      Retina, Degeneration                 |                         4                             3              4   |      4  3.5|
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  33                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 5| 5| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 4| 9| 6| 3| 3| 3| 8| 3| 2| 3| 3| 2| 3|            |
                                           | 7| 7| 7| 7| 7| 4| 4| 4| 4| 7| 6| 6| 0| 6| 0| 6| 6| 6| 7| 6| 9| 6| 6| 3| 6|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|      A     |
    25 MG/KG                               | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 SPECIAL SENSES SYSTEM - cont              |                                                                          |            |
      Inflammation, Chronic                |                         1                       2                        |      3  1.3|
      Pigmentation                         |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Zymbal's Gland                          |                                                       +                  |   1        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 |                                     2                                    |      1  2.0|
      Infarct                              |                                                                          |      2  1.5|
      Nephropathy                          | 3  1  1        1  1                          1     1           1        1|     21  1.2|
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  34                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 3| 7| 7| 2| 5| 7| 7| 6| 2| 2| 7| 7|             
                             DAY ON TEST   | 7| 2| 2| 2| 2| 3| 3| 3| 3| 4| 3| 3| 2| 4| 3| 3| 2| 7| 3| 3| 8| 8| 3| 3| 3|             
                                           | 7| 9| 9| 9| 5| 7| 7| 7| 7| 4| 6| 6| 7| 9| 6| 4| 3| 3| 4| 4| 7| 4| 9| 3| 3|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    50 MG/KG                               | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  A  +  +  +  +|             
      Cyst                                 |                                                                          |             
                                           |__________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Cecum                  | A  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  A  A  +  +  A  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  A  +  +  +  +|             
      Ectopic Tissue                       |                                                                          |             
                                           |__________________________________________________________________________|             
   Intestine Small, Jejunum                | A  +  +  +  A  +  +  +  +  +  +  +  +  A  +  +  A  A  +  +  A  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  A  A  +  +  A  +  +  +  A  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Basophilic Focus                     | X  X  X  X     X  X     X     X  X        X  X     X     X  X        X  X|             
      Clear Cell Focus                     |                X              X  X                                       |             
      Degeneration, Cystic                 |                                                          1               |             
      Hepatodiaphragmatic Nodule           |                      X                    2     2                    2   |             
      Inflammation, Chronic                |                                                                      2   |             
      Bile Duct, Hyperplasia               |             2                 2                                          |             
                                           |__________________________________________________________________________|             
   Mesentery                               | +                       +                                            +   |             
      Fat, Necrosis                        | 2                       2                                            3   |             
                                           |__________________________________________________________________________|             
   Oral Mucosa                             |                            +                                             |             
      Pharyngeal, Cyst                     |                            2                                             |             
                                           |__________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +|             
      Atrophy                              | 1     1  2  4     1  2              1     1     2  2                 2  2|             
      Acinus, Hyperplasia, Focal           |                                                                          |             
                                           |__________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  35                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 3| 7| 7| 2| 5| 7| 7| 6| 2| 2| 7| 7|             
                             DAY ON TEST   | 7| 2| 2| 2| 2| 3| 3| 3| 3| 4| 3| 3| 2| 4| 3| 3| 2| 7| 3| 3| 8| 8| 3| 3| 3|             
                                           | 7| 9| 9| 9| 5| 7| 7| 7| 7| 4| 6| 6| 7| 9| 6| 4| 3| 3| 4| 4| 7| 4| 9| 3| 3|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    50 MG/KG                               | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM - cont                  |                                                                          |             
      Erosion                              |                                                                          |             
      Inflammation, Chronic Active         |                                                                      1   |             
      Ulcer                                |             2                                                            |             
      Epithelium, Hyperplasia              |                                                                   2  1   |             
                                           |__________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Tongue                                  |                                                                          |             
      Hyperplasia                          |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Blood Vessel                            |                            +                                             |             
                                           |__________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       | 1  1  1  1        1  2  1  1     1        2  1        2  2           1   |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Accessory Adrenal Cortical Nodule    |                   2     2           1  1  1  1  1                    1   |             
      Degeneration, Fatty                  |             1     2     2                                                |             
      Hyperplasia, Focal                   |       2           2                 1                          2         |             
      Hypertrophy, Focal                   |             2     2     3                                                |             
                                           |__________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +|             
      Hyperplasia                          | 1     1                 1  1  1  1                             1     1   |             
                                           |__________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Pars Distalis, Angiectasis           | 2  2  1                          2           3     2  1                  |             
      Pars Distalis, Cyst                  | 2  2     2     2           1     2  1     2           2              2   |             
      Pars Distalis, Hyperplasia, Focal    |                         2  1                          2                  |             
                                           |__________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  36                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 3| 7| 7| 2| 5| 7| 7| 6| 2| 2| 7| 7|             
                             DAY ON TEST   | 7| 2| 2| 2| 2| 3| 3| 3| 3| 4| 3| 3| 2| 4| 3| 3| 2| 7| 3| 3| 8| 8| 3| 3| 3|             
                                           | 7| 9| 9| 9| 5| 7| 7| 7| 7| 4| 6| 6| 7| 9| 6| 4| 3| 3| 4| 4| 7| 4| 9| 3| 3|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    50 MG/KG                               | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ENDOCRINE SYSTEM - cont                   |                                                                          |             
      C-Cell, Hyperplasia                  |                                              2                           |             
      Follicle, Cyst                       |                                  3        1                              |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
    None                                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |    2                          2     2                    2              2|             
      Inflammation, Chronic                |                      3        2                                      2   |             
                                           |__________________________________________________________________________|             
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |    2                             2  2     2  1              2        1   |             
                                           |__________________________________________________________________________|             
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                       X                  |             
      Hemorrhage                           |                                           3                              |             
      Hydrometra                           |                                                                2         |             
      Endometrium, Hyperplasia, Cystic     |                                                                      2   |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                            2                                   2         |             
                                           |__________________________________________________________________________|             
   Lymph Node                              |                                                                +         |             
      Mediastinal, Hyperplasia, Lymphoid   |                                                                          |             
      Pancreatic, Hyperplasia, Lymphoid    |                                                                2         |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mandibular                  | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|             
                                           |__________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +|             
      Hematopoietic Cell Proliferation     |                      2     2  1                                      1   |             
      Capsule, Fibrosis                    |    1           1     1  1  1     1                 1  1  1           1   |             
                                           |__________________________________________________________________________|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  37                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 3| 7| 7| 2| 5| 7| 7| 6| 2| 2| 7| 7|             
                             DAY ON TEST   | 7| 2| 2| 2| 2| 3| 3| 3| 3| 4| 3| 3| 2| 4| 3| 3| 2| 7| 3| 3| 8| 8| 3| 3| 3|             
                                           | 7| 9| 9| 9| 5| 7| 7| 7| 7| 4| 6| 6| 7| 9| 6| 4| 3| 3| 4| 4| 7| 4| 9| 3| 3|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    50 MG/KG                               | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                          |             
      Galactocele                          |                                              X                           |             
      Hyperplasia                          |                                  2                                       |             
      Duct, Dilatation                     | 4  2  2  2        1                                2     3  2        2   |             
      Duct, Hyperplasia                    |                               2                                          |             
                                           |__________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Compression                          | 2                                                                        |             
                                           |__________________________________________________________________________|             
   Peripheral Nerve                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Spinal Cord                             |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                     1  1           2        2            |             
      Foreign Body                         |                                                                          |             
      Infiltration Cellular, Histiocyte    | 1  1  1  1  1     1  1  1     1  1           1     1  1     1        1  1|             
      Inflammation, Chronic                |          1     1  1     1     1  1        1  1  2     1     1  1     1  1|             
      Alveolar Epithelium, Hyperplasia     | 2  3                                                                     |             
                                           |__________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |                2                    1                                    |             
      Inflammation, Chronic                |                2                    2                                2   |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  38                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 3| 7| 7| 2| 5| 7| 7| 6| 2| 2| 7| 7|             
                             DAY ON TEST   | 7| 2| 2| 2| 2| 3| 3| 3| 3| 4| 3| 3| 2| 4| 3| 3| 2| 7| 3| 3| 8| 8| 3| 3| 3|             
                                           | 7| 9| 9| 9| 5| 7| 7| 7| 7| 4| 6| 6| 7| 9| 6| 4| 3| 3| 4| 4| 7| 4| 9| 3| 3|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    50 MG/KG                               | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 RESPIRATORY SYSTEM - cont                 |                                                                          |             
                                           |__________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Ear                                     |                            +                                             |             
                                           |__________________________________________________________________________|             
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cataract                             |                                                                          |             
      Cornea, Inflammation, Chronic        |                                                                          |             
      Retina, Degeneration                 |                                                                          |             
                                           |__________________________________________________________________________|             
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic                |                      1                                      1            |             
      Pigmentation                         |                                                                   2      |             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                               2                                          |             
      Infarct                              |                         2                                                |             
      Nephropathy                          |          1              1     1  1           1                       1   |             
                                           |__________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  39                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 5| 6| 4| 7| 3| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 3| 2| 2| 2| 0| 7| 8| 3| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|            |
                                           | 7| 7| 7| 7| 7| 9| 9| 9| 9| 7| 0| 7| 2| 8| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4|      A     |
    50 MG/KG                               | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Cyst                                 | 4                                                                        |      1  4.0|
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  44        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Ectopic Tissue                       |    2                                                                     |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  43        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +|  45        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Basophilic Focus                     | X  X  X  X  X  X  X  X  X  X  X  X     X  X  X  X  X  X  X  X  X  X  X  X|     40     |
      Clear Cell Focus                     | X  X  X           X                                                     X|      8     |
      Degeneration, Cystic                 |                                                                          |      1  1.0|
      Hepatodiaphragmatic Nodule           |                                                                          |      4  2.0|
      Inflammation, Chronic                |    2                          1  2                 2                     |      5  1.8|
      Bile Duct, Hyperplasia               |                                                                          |      2  2.0|
                                           |__________________________________________________________________________|____________|
   Mesentery                               |          +  +                             +     +                       +|   8        |
      Fat, Necrosis                        |          2  2                             2     4                       2|      8  2.4|
                                           |__________________________________________________________________________|____________|
   Oral Mucosa                             |                                                                          |   1        |
      Pharyngeal, Cyst                     |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Atrophy                              | 1        1  1                    1           1  1     2  3        1  1  1|     23  1.5|
      Acinus, Hyperplasia, Focal           |                            2                                             |      1  2.0|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  40                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 5| 6| 4| 7| 3| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 3| 2| 2| 2| 0| 7| 8| 3| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|            |
                                           | 7| 7| 7| 7| 7| 9| 9| 9| 9| 7| 0| 7| 2| 8| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4|      A     |
    50 MG/KG                               | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Erosion                              |             1                                                            |      1  1.0|
      Inflammation, Chronic Active         |             1                                                            |      2  1.0|
      Ulcer                                |                                                                          |      1  2.0|
      Epithelium, Hyperplasia              |          1                                                               |      3  1.3|
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Tongue                                  |                                              +                           |   1        |
      Hyperplasia                          |                                              2                           |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Blood Vessel                            |                +                                                         |   2        |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cardiomyopathy                       | 1  1  1  1  1  1  1              1        1  1  1  1  1  1  1  1  2     1|     32  1.2|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Accessory Adrenal Cortical Nodule    |    2                                            1              1     1   |     12  1.3|
      Degeneration, Fatty                  |          1                                      1  2        1           1|      8  1.4|
      Hyperplasia, Focal                   |                                                    2                    3|      6  2.0|
      Hypertrophy, Focal                   |          1                                      2  2        2            |      7  2.0|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Hyperplasia                          |       1           1        1                 1        1     1        1   |     15  1.0|
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  41                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 5| 6| 4| 7| 3| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 3| 2| 2| 2| 0| 7| 8| 3| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|            |
                                           | 7| 7| 7| 7| 7| 9| 9| 9| 9| 7| 0| 7| 2| 8| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4|      A     |
    50 MG/KG                               | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ENDOCRINE SYSTEM - cont                   |                                                                          |            |
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Pars Distalis, Angiectasis           | 2  2  2  2        2              4        2  2  2     2  2     3     2  1|     21  2.0|
      Pars Distalis, Cyst                  |    2     2           1        1              2  3  3  2  3  2     2  2  2|     23  2.0|
      Pars Distalis, Hyperplasia, Focal    |                                        2                    2     2      |      6  1.8|
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      C-Cell, Hyperplasia                  |                   2           1  3        2                              |      5  2.0|
      Follicle, Cyst                       |                                  2                             2         |      4  2.0|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 |                                                             2            |      6  2.0|
      Inflammation, Chronic                |                2                                                         |      4  2.3|
                                           |__________________________________________________________________________|____________|
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 |       3                                                  2           1   |     10  1.8|
                                           |__________________________________________________________________________|____________|
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 | X        X                                                               |      3     |
      Hemorrhage                           |                                                                          |      1  3.0|
      Hydrometra                           |                                                                          |      1  2.0|
      Endometrium, Hyperplasia, Cystic     |                                                    2                     |      2  2.0|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |                         2        4     2                       3         |      6  2.5|
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  42                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 5| 6| 4| 7| 3| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 3| 2| 2| 2| 0| 7| 8| 3| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|            |
                                           | 7| 7| 7| 7| 7| 9| 9| 9| 9| 7| 0| 7| 2| 8| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4|      A     |
    50 MG/KG                               | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
   Lymph Node                              |                                                             +           +|   3        |
      Mediastinal, Hyperplasia, Lymphoid   |                                                             2           2|      2  2.0|
      Pancreatic, Hyperplasia, Lymphoid    |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|            |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Hematopoietic Cell Proliferation     |                   2              2                             2     1   |      8  1.6|
      Capsule, Fibrosis                    |    1        1     1                    1        1        1  1     1  1  1|     20  1.0|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 |                      1                                                   |      1  1.0|
      Galactocele                          |                                                                          |      1     |
      Hyperplasia                          |          2     2  2                                4  2           4      |      7  2.6|
      Duct, Dilatation                     | 2  2  2     2        1           4        2  2  3     2  4     3  2  4  2|     24  2.4|
      Duct, Hyperplasia                    |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Compression                          |                                  2              2        2     2         |      5  2.0|
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  43                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 5| 6| 4| 7| 3| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 3| 2| 2| 2| 0| 7| 8| 3| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|            |
                                           | 7| 7| 7| 7| 7| 9| 9| 9| 9| 7| 0| 7| 2| 8| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4|      A     |
    50 MG/KG                               | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 NERVOUS SYSTEM - cont                     |                                                                          |            |
   Peripheral Nerve                        |                                                             +  +         |   2        |
                                           |__________________________________________________________________________|____________|
   Spinal Cord                             |                                                             +  +         |   2        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Congestion                           |                1              2     2        1                           |      8  1.5|
      Foreign Body                         |                               3                                          |      1  3.0|
      Infiltration Cellular, Histiocyte    | 1  1  1     1  1  1  1  1  1  1  1     1  1  1  1  1  1     1  1  1  1  1|     38  1.0|
      Inflammation, Chronic                | 1  1  1  1  1  1     1  1  1  1  1        1  1  1  1  1     1     1     1|     33  1.0|
      Alveolar Epithelium, Hyperplasia     |                                                                          |      2  2.5|
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Foreign Body                         |    2                                                                     |      3  1.7|
      Inflammation, Chronic                |    2     1  1                                                            |      6  1.7|
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Ear                                     |                                     +                 +                  |   3        |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cataract                             |                                                             2  2     2   |      3  2.0|
      Cornea, Inflammation, Chronic        |                                                             2        3   |      2  2.5|
      Retina, Degeneration                 |                                                             4  4         |      2  4.0|
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation, Chronic                |                            1  1                                          |      4  1.0|
      Pigmentation                         |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  44                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 5| 6| 4| 7| 3| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 3| 2| 2| 2| 0| 7| 8| 3| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|            |
                                           | 7| 7| 7| 7| 7| 9| 9| 9| 9| 7| 0| 7| 2| 8| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4|      A     |
    50 MG/KG                               | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 URINARY SYSTEM - cont                     |                                                                          |            |
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 |                                                                          |      1  2.0|
      Infarct                              |                                              2                           |      2  2.0|
      Nephropathy                          |          1     1  1  1                       1  1  1  1        1        1|     16  1.0|
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  45                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 5| 7| 7| 7| 7| 4| 7| 6| 7| 7| 6| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 7| 7|             
                             DAY ON TEST   | 3| 3| 6| 3| 3| 3| 3| 5| 3| 4| 3| 3| 6| 0| 3| 6| 3| 7| 3| 3| 3| 9| 4| 3| 3|             
                                           | 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 1| 1| 2| 2| 0| 2| 3| 5| 2| 2| 2| 4| 2| 3| 3|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Ectopic Tissue                       |                                                                          |             
                                           |__________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Basophilic Focus                     |    X        X                    X                                   X   |             
      Clear Cell Focus                     |    X              X           X  X        X     X                    X  X|             
      Degeneration, Cystic                 |                                                 1                        |             
      Eosinophilic Focus                   |             X                             X              X  X            |             
      Hepatodiaphragmatic Nodule           |    X     2  3                          2                                2|             
      Necrosis, Focal                      |                                                                          |             
      Bile Duct, Hyperplasia               |    2  1  2  2  3  2     4  2  3  2  3  2  2     3  3  3  3  3  3  2  3  2|             
      Centrilobular, Necrosis              | 2                                                                        |             
      Hepatocyte, Vacuolization Cytoplasmic|                                              2     2           3         |             
                                           |__________________________________________________________________________|             
   Mesentery                               | +     +     +  +        +  +                    +                        |             
      Fibrosis                             |                                                                          |             
      Fat, Necrosis                        | 2     3     3  3        3                       3                        |             
                                           |__________________________________________________________________________|             
   Oral Mucosa                             |                                                                          |             
                                           |__________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              | 2     2  4  1  1  3     2  2  1  4  4  2  1     3     2  1  2  2  2  1  2|             
      Acinus, Hyperplasia, Focal           |                                                             2            |             
                                           |__________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  46                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 5| 7| 7| 7| 7| 4| 7| 6| 7| 7| 6| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 7| 7|             
                             DAY ON TEST   | 3| 3| 6| 3| 3| 3| 3| 5| 3| 4| 3| 3| 6| 0| 3| 6| 3| 7| 3| 3| 3| 9| 4| 3| 3|             
                                           | 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 1| 1| 2| 2| 0| 2| 3| 5| 2| 2| 2| 4| 2| 3| 3|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM - cont                  |                                                                          |             
                                           |__________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Edema                                |                         2           2              4                     |             
      Erosion                              |                         2              2                       3         |             
      Ulcer                                |                                                    4                     |             
      Epithelium, Hyperplasia              |                         2              2                       3         |             
                                           |__________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Edema                                |                                     2                                    |             
      Erosion                              | 2                                   2                                    |             
      Hyperplasia                          |                                                 2                        |             
      Inflammation                         |                                                 2                        |             
      Ulcer                                |                                              3                           |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Blood Vessel                            |                                  +              +                        |             
                                           |__________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       | 1  1  2  2  2  2  2     1  2  2  1  1  1  2  1  1  2  2  2  2  1     2  2|             
      Pericardium, Inflammation,           |                                                                          |             
          Granulomatous                    |                      3                                                   |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Accessory Adrenal Cortical Nodule    |    1     1        1     1        1  1     1           1  2  1            |             
      Degeneration, Fatty                  |          2        1                                                      |             
      Hyperplasia, Focal                   |             1     1                          1           1               |             
      Hypertrophy, Focal                   |                            3                       2           2         |             
      Necrosis                             |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                            2        2  2                    3        2   |             
                                           |__________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                           1  1                 2     1  1|             
                                           |__________________________________________________________________________|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  47                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 5| 7| 7| 7| 7| 4| 7| 6| 7| 7| 6| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 7| 7|             
                             DAY ON TEST   | 3| 3| 6| 3| 3| 3| 3| 5| 3| 4| 3| 3| 6| 0| 3| 6| 3| 7| 3| 3| 3| 9| 4| 3| 3|             
                                           | 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 1| 1| 2| 2| 0| 2| 3| 5| 2| 2| 2| 4| 2| 3| 3|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ENDOCRINE SYSTEM - cont                   |                                                                          |             
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Pars Distalis, Angiectasis           |             3  3           2     2                          2  2         |             
      Pars Distalis, Cyst                  |                                                 X  4        2        2   |             
      Pars Distalis, Hyperplasia, Focal    |       2                 2                 2                              |             
      Pars Intermedia, Cyst                |                                                                          |             
                                           |__________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Ultimobranchial Cyst                 |                                        2                                 |             
      C-Cell, Hyperplasia                  |                                        2  3     3                        |             
      Follicle, Cyst                       |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |__________________________________________________________________________|             
   Peritoneum                              |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Coagulating Gland                       |                                                                          |             
                                           |__________________________________________________________________________|             
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Penis                                   |                                                                          |             
      Fibrosis                             |                                                                          |             
                                           |__________________________________________________________________________|             
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 | X                                         X              3               |             
      Inflammation, Chronic                |    1                                      2              3               |             
                                           |__________________________________________________________________________|             
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic                | 3     2     2  4        2  2     1  2           2  3  2  2  1  2  1  2  2|             
                                           |__________________________________________________________________________|             
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Bilateral, Germinal Epithelium,      |                                                                          |             
          Atrophy                          |                                                                4         |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  48                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 5| 7| 7| 7| 7| 4| 7| 6| 7| 7| 6| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 7| 7|             
                             DAY ON TEST   | 3| 3| 6| 3| 3| 3| 3| 5| 3| 4| 3| 3| 6| 0| 3| 6| 3| 7| 3| 3| 3| 9| 4| 3| 3|             
                                           | 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 1| 1| 2| 2| 0| 2| 3| 5| 2| 2| 2| 4| 2| 3| 3|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 GENITAL SYSTEM - cont                     |                                                                          |             
      Germinal Epithelium, Atrophy         |                                                             1            |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |       2  3                       2     3                          2  2   |             
                                           |__________________________________________________________________________|             
   Lymph Node                              | +           +  +     +  +     +     +        +     +  +     +  +         |             
      Deep Cervical, Inflammation,         |                                                                          |             
          Granulomatous                    |                      2                                                   |             
      Mediastinal, Hyperplasia, Lymphoid   |                2        2     2                    2        2            |             
      Mediastinal, Inflammation,           |                                                                          |             
          Granulomatous                    |                      3                                                   |             
      Pancreatic, Hyperplasia, Lymphoid    |             2                                                            |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mandibular                  | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|             
                                           |__________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Lymphoid                |    2                                   2  1              2  2            |             
                                           |__________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Fibrosis                             |                                                                2         |             
      Hematopoietic Cell Proliferation     |       2  1                       1     3           2     1               |             
      Necrosis                             |                                                                          |             
      Capsule, Fibrosis                    |                                                                          |             
                                           |__________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |    X        X  X        X  X     X     X  X  X     1  1     2  2         |             
      Hyperplasia                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Lip, Inflammation                    |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  49                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 5| 7| 7| 7| 7| 4| 7| 6| 7| 7| 6| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 7| 7|             
                             DAY ON TEST   | 3| 3| 6| 3| 3| 3| 3| 5| 3| 4| 3| 3| 6| 0| 3| 6| 3| 7| 3| 3| 3| 9| 4| 3| 3|             
                                           | 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 1| 1| 2| 2| 0| 2| 3| 5| 2| 2| 2| 4| 2| 3| 3|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM - cont             |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                          |             
                                           |__________________________________________________________________________|             
   Skeletal Muscle                         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Compression                          |                2                                   3        3  3         |             
      Hemorrhage                           |                                     3                                    |             
                                           |__________________________________________________________________________|             
   Peripheral Nerve                        |       +        +                                                         |             
                                           |__________________________________________________________________________|             
   Spinal Cord                             |                +                                                         |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                      2                                            2      |             
      Hemorrhage                           |                         2                                                |             
      Infiltration Cellular, Histiocyte    |    1  1  1  1  1  1     1        1     1           1     1  1  1  1  1  1|             
      Inflammation, Chronic                |       1  1              1  1     1     3        1                    1  1|             
      Alveolar Epithelium, Hyperplasia     |                                  2                       2        2     4|             
      Mediastinum, Inflammation,           |                                                                          |             
          Granulomatous                    |                      3                                                   |             
                                           |__________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |                                           2  2        X           2      |             
      Inflammation, Chronic                |    3  2                 2  1                 2        3           2     2|             
                                           |__________________________________________________________________________|             
   Pleura                                  |                      +                                                   |             
      Inflammation, Granulomatous          |                      3                                                   |             
                                           |__________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |                                        3                                 |             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  50                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 5| 7| 7| 7| 7| 4| 7| 6| 7| 7| 6| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 7| 7|             
                             DAY ON TEST   | 3| 3| 6| 3| 3| 3| 3| 5| 3| 4| 3| 3| 6| 0| 3| 6| 3| 7| 3| 3| 3| 9| 4| 3| 3|             
                                           | 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 1| 1| 2| 2| 0| 2| 3| 5| 2| 2| 2| 4| 2| 3| 3|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 SPECIAL SENSES SYSTEM - cont              |                                                                          |             
                                           |__________________________________________________________________________|             
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cataract                             |                                                                          |             
      Inflammation, Chronic                |                                                                          |             
      Retina, Degeneration                 |                                                                          |             
                                           |__________________________________________________________________________|             
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic                |                                                                   2      |             
      Pigmentation                         |                                                    2                     |             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Infarct                              |          1                                   2                           |             
      Nephropathy                          | 3  2     1  3  3  2     1  1  1  1     1  1     1  2     1  2  2  2  2   |             
                                           |__________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           | 2                                                                        |             
 __________________________________________|__________________________________________________________________________              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  51                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 6| 6| 6| 7| 7| 6| 5| 6| 5| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 2| 4| 9| 3| 2| 8| 8| 4| 8| 3| 3| 1| 3| 3| 3| 1| 3| 4| 3| 3| 3| 3|            |
                                           | 3| 3| 1| 0| 1| 4| 0| 9| 5| 6| 9| 6| 0| 0| 2| 2| 2| 0| 6| 0| 4| 2| 2| 0| 0|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Ectopic Tissue                       |                                                    X                     |      1     |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  A  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Basophilic Focus                     |                                                    X                     |      5     |
      Clear Cell Focus                     | X                                   X  X     X  X  X                     |     14     |
      Degeneration, Cystic                 | 2        2                          2                 2                  |      5  1.8|
      Eosinophilic Focus                   |       X                                                                  |      5     |
      Hepatodiaphragmatic Nodule           |       2                                   2        2           2         |      9  2.1|
      Necrosis, Focal                      |                                                       2                  |      1  2.0|
      Bile Duct, Hyperplasia               | 3  2  4  2  2  2  2  2           2  2  3  2  2  2  3  4        2     3  3|     41  2.5|
      Centrilobular, Necrosis              |                         1                                                |      2  1.5|
      Hepatocyte, Vacuolization Cytoplasmic|                                                 1           3            |      5  2.2|
                                           |__________________________________________________________________________|____________|
   Mesentery                               |    +        +              +  +     +        +           +  +     +      |  16        |
      Fibrosis                             |                            2                                             |      1  2.0|
      Fat, Necrosis                        |    2        3              3  2     3                    2  2     2      |     14  2.6|
                                           |__________________________________________________________________________|____________|
   Oral Mucosa                             |                      +                                                   |   1        |
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  52                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 6| 6| 6| 7| 7| 6| 5| 6| 5| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 2| 4| 9| 3| 2| 8| 8| 4| 8| 3| 3| 1| 3| 3| 3| 1| 3| 4| 3| 3| 3| 3|            |
                                           | 3| 3| 1| 0| 1| 4| 0| 9| 5| 6| 9| 6| 0| 0| 2| 2| 2| 0| 6| 0| 4| 2| 2| 0| 0|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
      Atrophy                              | 2  1  4  2  2     1  1  1     2  1  2  1     3  2  3  2  3  4  1  1  2  4|     43  2.1|
      Acinus, Hyperplasia, Focal           |    2                                   2              2                  |      4  2.0|
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Edema                                |                      2           3                          2            |      6  2.5|
      Erosion                              |                                                                          |      3  2.3|
      Ulcer                                |                      2     4     4                                       |      4  3.5|
      Epithelium, Hyperplasia              |                                                             2            |      4  2.3|
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Edema                                |                                                                          |      1  2.0|
      Erosion                              |                         2                                   2            |      4  2.0|
      Hyperplasia                          |                                                                          |      1  2.0|
      Inflammation                         |                                                                          |      1  2.0|
      Ulcer                                |                            4                                             |      2  3.5|
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Blood Vessel                            |             +  +                                                         |   4        |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cardiomyopathy                       | 2  2  2  2  1  2  1  2  2  1  2  2  1  1  1  1  1  2  2  2     2  2  2  2|     47  1.6|
      Pericardium, Inflammation,           |                                                                          |            |
          Granulomatous                    |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Accessory Adrenal Cortical Nodule    | 1  1  1     1     1           1     1  1     1  1  1                 1  2|     23  1.1|
      Degeneration, Fatty                  |                                              1     2     2  3           2|      7  1.9|
      Hyperplasia, Focal                   | 1                                                                       2|      6  1.2|
      Hypertrophy, Focal                   |                                              2     3                    2|      6  2.3|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  53                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 6| 6| 6| 7| 7| 6| 5| 6| 5| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 2| 4| 9| 3| 2| 8| 8| 4| 8| 3| 3| 1| 3| 3| 3| 1| 3| 4| 3| 3| 3| 3|            |
                                           | 3| 3| 1| 0| 1| 4| 0| 9| 5| 6| 9| 6| 0| 0| 2| 2| 2| 0| 6| 0| 4| 2| 2| 0| 0|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ENDOCRINE SYSTEM - cont                   |                                                                          |            |
      Necrosis                             |          2                                                               |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |                                              1                 2         |      7  2.0|
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |    1  1  2  1     1              1  2              1                     |     13  1.2|
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Pars Distalis, Angiectasis           |    2  2     2  2  2     2     2                    2                     |     14  2.1|
      Pars Distalis, Cyst                  | 2                                   2                                    |      6  2.4|
      Pars Distalis, Hyperplasia, Focal    | 3        2                          2                    2  2  2         |      9  2.1|
      Pars Intermedia, Cyst                |                                                                         1|      1  1.0|
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Ultimobranchial Cyst                 |       2                                                  1               |      3  1.7|
      C-Cell, Hyperplasia                  |    3              3                    1                             2  2|      8  2.4|
      Follicle, Cyst                       |                                        1                                 |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Peritoneum                              |                                              +                           |   1        |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Coagulating Gland                       |                +                                                         |   1        |
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Penis                                   |                               +                                          |   1        |
      Fibrosis                             |                               2                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  54                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 6| 6| 6| 7| 7| 6| 5| 6| 5| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 2| 4| 9| 3| 2| 8| 8| 4| 8| 3| 3| 1| 3| 3| 3| 1| 3| 4| 3| 3| 3| 3|            |
                                           | 3| 3| 1| 0| 1| 4| 0| 9| 5| 6| 9| 6| 0| 0| 2| 2| 2| 0| 6| 0| 4| 2| 2| 0| 0|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM - cont                     |                                                                          |            |
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 |          2                                                               |      4  2.5|
      Inflammation, Chronic                |    2                                                                 2   |      5  2.0|
                                           |__________________________________________________________________________|____________|
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation, Chronic                | 1  2  2  3  4  1  1  2        3  3        1           1        1     3   |     31  2.0|
                                           |__________________________________________________________________________|____________|
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Bilateral, Germinal Epithelium,      |                                                                          |            |
          Atrophy                          |                                                                          |      1  4.0|
      Germinal Epithelium, Atrophy         |                                                                          |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |                                           3                              |      7  2.4|
                                           |__________________________________________________________________________|____________|
   Lymph Node                              |    +     +     +     +     +  +        +                 +  +            |  21        |
      Deep Cervical, Inflammation,         |                                                                          |            |
          Granulomatous                    |                                                                          |      1  2.0|
      Mediastinal, Hyperplasia, Lymphoid   |    2                          2        2                 2               |      9  2.0|
      Mediastinal, Inflammation,           |                                                                          |            |
          Granulomatous                    |                                                                          |      1  3.0|
      Pancreatic, Hyperplasia, Lymphoid    |    2                                                                     |      2  2.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | M  M  M  M  +  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|   1        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia, Lymphoid                | 4  3                                                                     |      7  2.3|
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Fibrosis                             |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  55                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 6| 6| 6| 7| 7| 6| 5| 6| 5| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 2| 4| 9| 3| 2| 8| 8| 4| 8| 3| 3| 1| 3| 3| 3| 1| 3| 4| 3| 3| 3| 3|            |
                                           | 3| 3| 1| 0| 1| 4| 0| 9| 5| 6| 9| 6| 0| 0| 2| 2| 2| 0| 6| 0| 4| 2| 2| 0| 0|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
      Hematopoietic Cell Proliferation     |             2                             2              1     1  1      |     11  1.5|
      Necrosis                             |          2              4                                                |      2  3.0|
      Capsule, Fibrosis                    |             1                                                            |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +|  49        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 | 3  2        2  2  2  3  1     1                    2     1               |     23  1.8|
      Hyperplasia                          |                                                                      2   |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Lip, Inflammation                    |                      4                                                   |      1  4.0|
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 |                                  X                                       |      1     |
                                           |__________________________________________________________________________|____________|
   Skeletal Muscle                         |                   +              +                       +               |   3        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Compression                          |             3  3              4                                          |      7  3.0|
      Hemorrhage                           |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Peripheral Nerve                        |                   +  +           +                    +                  |   6        |
                                           |__________________________________________________________________________|____________|
   Spinal Cord                             |                   +              +                    +                  |   4        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  56                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 6| 6| 6| 7| 7| 6| 5| 6| 5| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 2| 4| 9| 3| 2| 8| 8| 4| 8| 3| 3| 1| 3| 3| 3| 1| 3| 4| 3| 3| 3| 3|            |
                                           | 3| 3| 1| 0| 1| 4| 0| 9| 5| 6| 9| 6| 0| 0| 2| 2| 2| 0| 6| 0| 4| 2| 2| 0| 0|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 RESPIRATORY SYSTEM - cont                 |                                                                          |            |
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Congestion                           |                                                          1               |      3  1.7|
      Hemorrhage                           |                                                                          |      1  2.0|
      Infiltration Cellular, Histiocyte    | 2  1  1     1     1  1                 1  1  1  1                 2     1|     28  1.1|
      Inflammation, Chronic                | 1  1  2     1  1  1     1  1  2     1  1  1  1  1  1        1  1  1  1  1|     29  1.1|
      Alveolar Epithelium, Hyperplasia     |          2  1                                      1                     |      7  2.0|
      Mediastinum, Inflammation,           |                                                                          |            |
          Granulomatous                    |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Foreign Body                         | 2     2                             2                                    |      7  2.0|
      Inflammation, Chronic                | 2     2                                2           1                     |     12  2.0|
                                           |__________________________________________________________________________|____________|
   Pleura                                  |                                                                          |   1        |
      Inflammation, Granulomatous          |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation                         |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cataract                             |                                                       2                  |      1  2.0|
      Inflammation, Chronic                |                      4                                                   |      1  4.0|
      Retina, Degeneration                 |                                                       2                  |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation, Chronic                |                      2                                                   |      2  2.0|
      Pigmentation                         |             2                                                            |      2  2.0|
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Infarct                              |                                                                          |      2  1.5|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  57                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 6| 6| 6| 7| 7| 6| 5| 6| 5| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 2| 4| 9| 3| 2| 8| 8| 4| 8| 3| 3| 1| 3| 3| 3| 1| 3| 4| 3| 3| 3| 3|            |
                                           | 3| 3| 1| 0| 1| 4| 0| 9| 5| 6| 9| 6| 0| 0| 2| 2| 2| 0| 6| 0| 4| 2| 2| 0| 0|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 URINARY SYSTEM - cont                     |                                                                          |            |
      Nephropathy                          | 3  2  1     1  3              2  1  1  1  1     2  2  3        1  2  3   |     35  1.7|
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hemorrhage                           |                                                                          |      1  2.0|
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  58                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 6| 7| 5| 4| 7| 7| 7| 6| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 5|             
                             DAY ON TEST   | 7| 3| 8| 3| 7| 7| 3| 3| 3| 5| 3| 3| 3| 3| 3| 6| 2| 3| 3| 3| 3| 3| 3| 3| 4|             
                                           | 3| 2| 2| 0| 6| 3| 2| 2| 2| 2| 2| 2| 1| 1| 1| 1| 0| 2| 2| 2| 2| 2| 2| 2| 0|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    5 MG/KG                                | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Ectopic Tissue                       |          2                                                               |             
                                           |__________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  A  +  +  +  +  +  +  +  A|             
                                           |__________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Basophilic Focus                     |                                  X  X           X                        |             
      Clear Cell Focus                     |    X              X           X  X     X                 X           X   |             
      Degeneration, Cystic                 |          1                                                               |             
      Eosinophilic Focus                   |                                                                          |             
      Hepatodiaphragmatic Nodule           |                2        2                                                |             
      Inflammation, Chronic                |                                                                          |             
      Necrosis, Focal                      |    1                                                                     |             
      Bile Duct, Hyperplasia               |          3     2  2  2  1  2  2  2  2     2  2     3     2  2  3  2  2   |             
      Hepatocyte, Vacuolization Cytoplasmic|                            2                                             |             
                                           |__________________________________________________________________________|             
   Mesentery                               |                      +     +              +                              |             
      Fat, Necrosis                        |                      2     2              3                              |             
                                           |__________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              | 2     2  3  1     2  1     2        2  2     1     2     1           3  1|             
      Acinus, Hyperplasia, Focal           |                               1  2                 2                 1   |             
                                           |__________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Ulcer                                |       2                                                                  |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  59                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 6| 7| 5| 4| 7| 7| 7| 6| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 5|             
                             DAY ON TEST   | 7| 3| 8| 3| 7| 7| 3| 3| 3| 5| 3| 3| 3| 3| 3| 6| 2| 3| 3| 3| 3| 3| 3| 3| 4|             
                                           | 3| 2| 2| 0| 6| 3| 2| 2| 2| 2| 2| 2| 1| 1| 1| 1| 0| 2| 2| 2| 2| 2| 2| 2| 0|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    5 MG/KG                                | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM - cont                  |                                                                          |             
                                           |__________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Erosion                              |                            3                    3                        |             
      Mineralization                       |       2                                                                  |             
      Ulcer                                |                                                                2         |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       | 2  1  2  1  1  2  2  2  2  1  1  2  1  1  1  2  1  1  1  2  1  1  1  1  2|             
      Mineralization                       |       3                                                                  |             
      Thrombosis                           |                                                 2                        |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Accessory Adrenal Cortical Nodule    | 2        1        1     1           1     1  1  1  1              2  1   |             
      Degeneration, Fatty                  |          1                                                               |             
      Hyperplasia, Focal                   |          2                 1                                             |             
      Hypertrophy, Focal                   |       1                                                                  |             
                                           |__________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |          1        2           2                 2     2        1  2  2   |             
                                           |__________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |    2  2     1           2  1           2     1        4           1      |             
                                           |__________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Pars Distalis, Angiectasis           | 2     2        2  2  2     2  2  2        2  2  2  2     2  2           2|             
      Pars Distalis, Cyst                  |    1                 1                                         2         |             
      Pars Distalis, Hyperplasia, Focal    |          2                          2                          3  1      |             
      Pars Distalis, Hyperplasia,          |                                                                          |             
          Multifocal                       |                                                                          |             
                                           |__________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Ultimobranchial Cyst                 |                                                       1                  |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  60                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 6| 7| 5| 4| 7| 7| 7| 6| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 5|             
                             DAY ON TEST   | 7| 3| 8| 3| 7| 7| 3| 3| 3| 5| 3| 3| 3| 3| 3| 6| 2| 3| 3| 3| 3| 3| 3| 3| 4|             
                                           | 3| 2| 2| 0| 6| 3| 2| 2| 2| 2| 2| 2| 1| 1| 1| 1| 0| 2| 2| 2| 2| 2| 2| 2| 0|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    5 MG/KG                                | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ENDOCRINE SYSTEM - cont                   |                                                                          |             
      C-Cell, Hyperplasia                  |                                                                   1      |             
      Follicle, Cyst                       |                                                    2                     |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |__________________________________________________________________________|             
   Peritoneum                              |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic                |                                                             2            |             
                                           |__________________________________________________________________________|             
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic                | 2     2  2  2  2     2     3              1  2     2     1  2     1      |             
                                           |__________________________________________________________________________|             
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          | 4           3                                   3        2               |             
                                           |__________________________________________________________________________|             
   Lymph Node                              | +                                +  +     +        +           +        +|             
      Deep Cervical, Hyperplasia, Lymphoid | 3                                                                        |             
      Mediastinal, Hyperplasia, Lymphoid   |                                  2  2     2                             2|             
      Pancreatic, Hyperplasia, Lymphoid    |                                     2                                    |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mandibular                  | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|             
                                           |__________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Lymphoid                |                      2                                                  2|             
                                           |__________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Fibrosis                             |          2                                                     1         |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  61                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 6| 7| 5| 4| 7| 7| 7| 6| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 5|             
                             DAY ON TEST   | 7| 3| 8| 3| 7| 7| 3| 3| 3| 5| 3| 3| 3| 3| 3| 6| 2| 3| 3| 3| 3| 3| 3| 3| 4|             
                                           | 3| 2| 2| 0| 6| 3| 2| 2| 2| 2| 2| 2| 1| 1| 1| 1| 0| 2| 2| 2| 2| 2| 2| 2| 0|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    5 MG/KG                                | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
      Hematopoietic Cell Proliferation     | 3     3  1  2                 1                 4     1                 2|             
      Capsule, Fibrosis                    |                   1           1                                          |             
      Lymphoid Follicle, Hyperplasia       |                                                                          |             
                                           |__________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 | 2     3     1  2           2                 2  1  1                     |             
                                           |__________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cranium, Osteopetrosis               |                      2                                                   |             
                                           |__________________________________________________________________________|             
   Skeletal Muscle                         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Compression                          |       2        2           2                 2     2        2            |             
      Hemorrhage                           |                1                                               2         |             
                                           |__________________________________________________________________________|             
   Peripheral Nerve                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Spinal Cord                             |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                2                             1                           |             
      Infiltration Cellular, Histiocyte    |    1  1     1     1  1  1  1        1     1        1  1  1  1     1  1   |             
      Inflammation, Chronic                |    1  1     1  1  1  1  1           1  1  1  1     1     1  1        1   |             
      Alveolar Epithelium, Hyperplasia     |    1                    2                                         3      |             
                                           |__________________________________________________________________________|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  62                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 6| 7| 5| 4| 7| 7| 7| 6| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 5|             
                             DAY ON TEST   | 7| 3| 8| 3| 7| 7| 3| 3| 3| 5| 3| 3| 3| 3| 3| 6| 2| 3| 3| 3| 3| 3| 3| 3| 4|             
                                           | 3| 2| 2| 0| 6| 3| 2| 2| 2| 2| 2| 2| 1| 1| 1| 1| 0| 2| 2| 2| 2| 2| 2| 2| 0|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    5 MG/KG                                | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 RESPIRATORY SYSTEM - cont                 |                                                                          |             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |                                                    1                     |             
      Inflammation, Chronic                |                                                    1                     |             
                                           |__________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +|             
      Cataract                             |                                                       3                  |             
      Bilateral, Anterior Chamber,         |                                                                          |             
          Inflammation, Acute              |       2                                                                  |             
      Retina, Degeneration                 |                                                       3                  |             
                                           |__________________________________________________________________________|             
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic                |          1                          1                 1                  |             
      Pigmentation                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Zymbal's Gland                          |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hydronephrosis                       |       3                                                                  |             
      Nephropathy                          | 2  2  3     1        1  1  3  1     2  2           3  1  1  2  1  1  1  1|             
                                           |__________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic                |       4                                                                  |             
 __________________________________________|__________________________________________________________________________              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  63                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 5| 5| 7| 5|            |
                             DAY ON TEST   | 5| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 7| 3| 4| 3| 2| 8| 9| 1| 6|            |
                                           | 2| 0| 0| 0| 0| 0| 0| 0| 3| 3| 3| 1| 1| 1| 0| 0| 3| 0| 0| 0| 2| 2| 0| 2| 8|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1|      A     |
    5 MG/KG                                | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Ectopic Tissue                       |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Basophilic Focus                     |                   X                          X           X               |      6     |
      Clear Cell Focus                     |          X  X           X           X              X                    X|     13     |
      Degeneration, Cystic                 |                         2     1                                          |      3  1.3|
      Eosinophilic Focus                   |                         X                                                |      1     |
      Hepatodiaphragmatic Nodule           |                                                                          |      2  2.0|
      Inflammation, Chronic                |                                                          1               |      1  1.0|
      Necrosis, Focal                      |                                                                          |      1  1.0|
      Bile Duct, Hyperplasia               |    3  3        2  2  2  2  4  2  2        2     3  3  2  2  2  2  2  3   |     35  2.3|
      Hepatocyte, Vacuolization Cytoplasmic|                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Mesentery                               |                   +              +  +     +  +     +     +        +      |  11        |
      Fat, Necrosis                        |                   2              3        2  1     3     3               |      9  2.3|
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Atrophy                              | 1  1        2  2  1  2  1  4     1     1  2     4  2  1           2  3  2|     31  1.8|
      Acinus, Hyperplasia, Focal           |                                                          2              2|      6  1.7|
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  64                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 5| 5| 7| 5|            |
                             DAY ON TEST   | 5| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 7| 3| 4| 3| 2| 8| 9| 1| 6|            |
                                           | 2| 0| 0| 0| 0| 0| 0| 0| 3| 3| 3| 1| 1| 1| 0| 0| 3| 0| 0| 0| 2| 2| 0| 2| 8|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1|      A     |
    5 MG/KG                                | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Ulcer                                |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Erosion                              |                                                                          |      2  3.0|
      Mineralization                       |                                                                          |      1  2.0|
      Ulcer                                |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cardiomyopathy                       | 2  2     1  2  2  1     1  2  2  2  2     1  2  1  2  1  1  1  1  1     2|     46  1.5|
      Mineralization                       |                                                                          |      1  3.0|
      Thrombosis                           |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Accessory Adrenal Cortical Nodule    |    2  2     1     1  1     1     1  1     1     1        1  1            |     23  1.2|
      Degeneration, Fatty                  | 1        1     1                    2     2                              |      6  1.3|
      Hyperplasia, Focal                   |          1     2                    2                                    |      5  1.6|
      Hypertrophy, Focal                   | 1                 2                 2     2                              |      5  1.6|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |                1  3              2              1                        |     12  1.8|
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |                2     1  1                                            2   |     13  1.7|
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  65                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 5| 5| 7| 5|            |
                             DAY ON TEST   | 5| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 7| 3| 4| 3| 2| 8| 9| 1| 6|            |
                                           | 2| 0| 0| 0| 0| 0| 0| 0| 3| 3| 3| 1| 1| 1| 0| 0| 3| 0| 0| 0| 2| 2| 0| 2| 8|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1|      A     |
    5 MG/KG                                | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ENDOCRINE SYSTEM - cont                   |                                                                          |            |
      Pars Distalis, Angiectasis           | 2  2  2           2  2  2     2  2        2  2     2  2  1     2     2   |     30  2.0|
      Pars Distalis, Cyst                  |                                                                          |      3  1.3|
      Pars Distalis, Hyperplasia, Focal    |             2                       1  2                 3               |      8  2.0|
      Pars Distalis, Hyperplasia,          |                                                                          |            |
          Multifocal                       |                      3                                                   |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Ultimobranchial Cyst                 |                                                                          |      1  1.0|
      C-Cell, Hyperplasia                  |             3                                                            |      2  2.0|
      Follicle, Cyst                       |    2                                                                     |      2  2.0|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Peritoneum                              |                                                                   +      |   1        |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation, Chronic                |                1                          1                 2        1   |      5  1.4|
                                           |__________________________________________________________________________|____________|
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation, Chronic                | 2  1  2  2     2  2        1  2  1  2     2  2        2        1     2   |     28  1.8|
                                           |__________________________________________________________________________|____________|
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |                                                          2        3     3|      7  2.9|
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  66                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 5| 5| 7| 5|            |
                             DAY ON TEST   | 5| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 7| 3| 4| 3| 2| 8| 9| 1| 6|            |
                                           | 2| 0| 0| 0| 0| 0| 0| 0| 3| 3| 3| 1| 1| 1| 0| 0| 3| 0| 0| 0| 2| 2| 0| 2| 8|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1|      A     |
    5 MG/KG                                | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
   Lymph Node                              |             +     +     +  +                 +  +  +  +           +  +   |  17        |
      Deep Cervical, Hyperplasia, Lymphoid |                                                                          |      1  3.0|
      Mediastinal, Hyperplasia, Lymphoid   |                   2                          2  2     2           2      |      9  2.0|
      Pancreatic, Hyperplasia, Lymphoid    |             2           2                          2                     |      4  2.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | M  M  +  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|   1        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia, Lymphoid                |                         2                                                |      3  2.0|
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Fibrosis                             |                                                                          |      2  1.5|
      Hematopoietic Cell Proliferation     | 2              1                                         1              3|     12  2.0|
      Capsule, Fibrosis                    |    1     2                       2           1     1                     |      7  1.3|
      Lymphoid Follicle, Hyperplasia       |                               2                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 | 2     1        3                    1     2  1     1  2        1     2   |     18  1.7|
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cranium, Osteopetrosis               |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Skeletal Muscle                         |                                                             +            |   1        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  67                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 5| 5| 7| 5|            |
                             DAY ON TEST   | 5| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 7| 3| 4| 3| 2| 8| 9| 1| 6|            |
                                           | 2| 0| 0| 0| 0| 0| 0| 0| 3| 3| 3| 1| 1| 1| 0| 0| 3| 0| 0| 0| 2| 2| 0| 2| 8|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1|      A     |
    5 MG/KG                                | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 NERVOUS SYSTEM - cont                     |                                                                          |            |
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Compression                          |                2                             2        2        2         |     10  2.0|
      Hemorrhage                           |                                                                          |      2  1.5|
                                           |__________________________________________________________________________|____________|
   Peripheral Nerve                        |                               +                             +     +      |   3        |
                                           |__________________________________________________________________________|____________|
   Spinal Cord                             |                                                             +            |   1        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Congestion                           |                                                       1        2         |      4  1.5|
      Infiltration Cellular, Histiocyte    |       1  1        1        1  1  1  1  1     1     1     1  2  1  1      |     29  1.0|
      Inflammation, Chronic                |                      1  1  1  1  1     1     1     1                     |     23  1.0|
      Alveolar Epithelium, Hyperplasia     |       2                    4  2                 2  2              2      |      9  2.2|
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Foreign Body                         |          1           1     1                                             |      4  1.0|
      Inflammation, Chronic                |                      1     2                                             |      3  1.3|
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Cataract                             |                            3  2                                          |      3  2.7|
      Bilateral, Anterior Chamber,         |                                                                          |            |
          Inflammation, Acute              |                                                                          |      1  2.0|
      Retina, Degeneration                 |                            3                                             |      2  3.0|
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation, Chronic                |                                                                          |      3  1.0|
      Pigmentation                         |                                                                2         |      1  2.0|
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  68                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 5| 5| 7| 5|            |
                             DAY ON TEST   | 5| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 7| 3| 4| 3| 2| 8| 9| 1| 6|            |
                                           | 2| 0| 0| 0| 0| 0| 0| 0| 3| 3| 3| 1| 1| 1| 0| 0| 3| 0| 0| 0| 2| 2| 0| 2| 8|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1|      A     |
    5 MG/KG                                | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 SPECIAL SENSES SYSTEM - cont              |                                                                          |            |
   Zymbal's Gland                          |                            +                                             |   1        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hydronephrosis                       |                                                                          |      1  3.0|
      Nephropathy                          | 3  2  2  1  1  3  2     1  1  3  2  2  2  1  4     4     1  2  1     1  1|     39  1.8|
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation, Chronic                |                                                             2            |      2  3.0|
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  69                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 5| 6| 6| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 4| 7| 6|             
                             DAY ON TEST   | 3| 3| 3| 3| 3| 8| 3| 1| 9| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3| 8| 3| 9|             
                                           | 0| 0| 0| 2| 2| 6| 7| 9| 0| 1| 1| 1| 3| 3| 3| 9| 0| 0| 2| 2| 2| 0| 9| 0| 0|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    25 MG/KG                               | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Ulcer                                |                      2                                                   |             
                                           |__________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  A|             
                                           |__________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Basophilic Focus                     |          X  X                                                  X         |             
      Clear Cell Focus                     | X     X              X           X  X              X     X               |             
      Degeneration, Cystic                 |                            1                                             |             
      Eosinophilic Focus                   |    X                 X              X                                    |             
      Hepatodiaphragmatic Nodule           |                                                                          |             
      Bile Duct, Hyperplasia               |       3  2  2           3  1  2  2        2  1  1  2  2  2  2  2     2  2|             
      Centrilobular, Necrosis              |                                                                          |             
      Hepatocyte, Vacuolization Cytoplasmic|                3                                                        2|             
                                           |__________________________________________________________________________|             
   Mesentery                               |       +           +                                         +           +|             
      Fat, Necrosis                        |       3           2                                         3           3|             
                                           |__________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |    4  1        2  2  2  2  3     2  4  1  2  2     2  2  2  1  1     1   |             
      Acinus, Hyperplasia, Focal           | 2                    2     1           2     2           2  1            |             
                                           |__________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Edema                                |                2                                                         |             
      Ulcer                                |                      3                                                  2|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  70                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 5| 6| 6| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 4| 7| 6|             
                             DAY ON TEST   | 3| 3| 3| 3| 3| 8| 3| 1| 9| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3| 8| 3| 9|             
                                           | 0| 0| 0| 2| 2| 6| 7| 9| 0| 1| 1| 1| 3| 3| 3| 9| 0| 0| 2| 2| 2| 0| 9| 0| 0|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    25 MG/KG                               | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM - cont                  |                                                                          |             
      Epithelium, Hyperplasia              |                2                                                        2|             
                                           |__________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Erosion                              |                      3                                                   |             
      Ulcer                                |                2                                                        2|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Blood Vessel                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       | 2  2  1  1  1  2  1     1  1  2  1  1  1  1  1  2  2  2  2  1  2  1  1  1|             
      Thrombosis                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Accessory Adrenal Cortical Nodule    | 1  1  1     1  1        1        1  1  1        2  1     1  1  1  1      |             
      Degeneration, Fatty                  |                                  2                          1           2|             
      Hyperplasia, Focal                   |    2                                                                     |             
      Hypertrophy, Focal                   |                         2        2                          2     2      |             
                                           |__________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |          1                                1     2                        |             
      Thrombosis                           |                                                                          |             
                                           |__________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          | 2              2        2                       1  2  2  1     1         |             
      Metaplasia, Hepatocyte               |                                              2                           |             
                                           |__________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Pars Distalis, Angiectasis           |    2     2  2  2  2           3                             2            |             
      Pars Distalis, Cyst                  |                                  1        2                    3        1|             
      Pars Distalis, Hyperplasia, Focal    |       1                          2                 1                 2   |             
                                           |__________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  71                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 5| 6| 6| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 4| 7| 6|             
                             DAY ON TEST   | 3| 3| 3| 3| 3| 8| 3| 1| 9| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3| 8| 3| 9|             
                                           | 0| 0| 0| 2| 2| 6| 7| 9| 0| 1| 1| 1| 3| 3| 3| 9| 0| 0| 2| 2| 2| 0| 9| 0| 0|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    25 MG/KG                               | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ENDOCRINE SYSTEM - cont                   |                                                                          |             
      C-Cell, Hyperplasia                  | 2                             2        2  1        2  1                  |             
      Follicle, Cyst                       |                                  1                       1               |             
      Follicular Cell, Hyperplasia         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |__________________________________________________________________________|             
   Peritoneum                              |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Coagulating Gland                       |                      +                                                   |             
      Inflammation                         |                      4                                                   |             
                                           |__________________________________________________________________________|             
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic                |                                                 1           2            |             
                                           |__________________________________________________________________________|             
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic                | 1  2  1     2  3  3  4  2  2  2     1     2           1     2     4  1   |             
                                           |__________________________________________________________________________|             
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Bilateral, Germinal Epithelium,      |                                                                          |             
          Atrophy                          |    2           4                                                         |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                   2                 4                             2      |             
                                           |__________________________________________________________________________|             
   Lymph Node                              |          +        +        +        +              +                     |             
      Deep Cervical, Hyperplasia, Lymphoid |                            4                                             |             
      Mediastinal, Hyperplasia, Lymphoid   |          2        2        2        2              2                     |             
      Pancreatic, Hyperplasia, Lymphoid    |                                                                          |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mandibular                  | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  72                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 5| 6| 6| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 4| 7| 6|             
                             DAY ON TEST   | 3| 3| 3| 3| 3| 8| 3| 1| 9| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3| 8| 3| 9|             
                                           | 0| 0| 0| 2| 2| 6| 7| 9| 0| 1| 1| 1| 3| 3| 3| 9| 0| 0| 2| 2| 2| 0| 9| 0| 0|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    25 MG/KG                               | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Lymphoid                |          2                                                               |             
                                           |__________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Fibrosis                             |                                                                          |             
      Hematopoietic Cell Proliferation     | 1  1           3              1     4     1  1                 1         |             
      Capsule, Fibrosis                    |                1           2     1        1        2     1               |             
      Lymphoid Follicle, Hyperplasia       |                                                                          |             
                                           |__________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |          2     2  2  2  2     3     1     2        1        1           1|             
      Hyperplasia                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic                |                   2        2           4                                 |             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Compression                          |          2     2  2     2     2                                          |             
                                           |__________________________________________________________________________|             
   Peripheral Nerve                        | +                    +     +                          +                  |             
                                           |__________________________________________________________________________|             
   Spinal Cord                             |                      +     +                          +                  |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                   1     1                                                |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  73                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 5| 6| 6| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 4| 7| 6|             
                             DAY ON TEST   | 3| 3| 3| 3| 3| 8| 3| 1| 9| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3| 8| 3| 9|             
                                           | 0| 0| 0| 2| 2| 6| 7| 9| 0| 1| 1| 1| 3| 3| 3| 9| 0| 0| 2| 2| 2| 0| 9| 0| 0|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    25 MG/KG                               | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 RESPIRATORY SYSTEM - cont                 |                                                                          |             
      Foreign Body                         |                                                                   2      |             
      Infiltration Cellular, Histiocyte    |    1     1  1  1  1        1  1     1  1  1  1  1  1  1  1  1  1         |             
      Inflammation, Chronic                | 1     1  1     1  1        1  1  1  1  1  1  1  1  1  1  1  1  1     1   |             
      Necrosis                             |                                                                   3      |             
      Alveolar Epithelium, Hyperplasia     |                                  3  2     1                              |             
                                           |__________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |          2                 2                 1                          2|             
      Inflammation, Chronic                |          2     2        2  2        1        1     1        1           2|             
                                           |__________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |                                                                   2      |             
      Perforation                          |                                                                   2      |             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Eye                                     | +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +|             
      Cataract                             |                      2     3                    2     4                  |             
      Inflammation, Chronic                |                            2                                             |             
      Retina, Degeneration                 |                      3     4                    3     4                  |             
                                           |__________________________________________________________________________|             
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                               2                                          |             
      Inflammation, Chronic                |             1                                                            |             
      Pigmentation                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Zymbal's Gland                          |                   +                                                      |             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                          |             
      Nephropathy                          | 1  2     1  1  3     1  1  1  1           1  1     2        1  1         |             
                                           |__________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          | 3                                                                        |             
 __________________________________________|__________________________________________________________________________              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  74                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 1| 1| 3| 3| 3| 1| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|            |
                                           | 1| 1| 3| 3| 0| 9| 0| 0| 0| 2| 6| 1| 1| 1| 1| 1| 1| 0| 0| 0| 3| 3| 3| 1| 1|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|      A     |
    25 MG/KG                               | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Ulcer                                |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Basophilic Focus                     |                      X                                      X  X  X     X|      8     |
      Clear Cell Focus                     |    X  X  X           X              X  X  X           X     X            |     16     |
      Degeneration, Cystic                 | 2                                                                        |      2  1.5|
      Eosinophilic Focus                   |                            X                                             |      4     |
      Hepatodiaphragmatic Nodule           |                         2                 X                       3      |      3  2.5|
      Bile Duct, Hyperplasia               | 2  2  2     1     1  2  2  2  2  2     2  2  2  3  2  1  2  2  2     2  2|     38  1.9|
      Centrilobular, Necrosis              |                2                                                         |      1  2.0|
      Hepatocyte, Vacuolization Cytoplasmic|                                                                          |      2  2.5|
                                           |__________________________________________________________________________|____________|
   Mesentery                               |             +  +        +        +                          +            |   9        |
      Fat, Necrosis                        |             3           4        3                          3            |      8  3.0|
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Atrophy                              | 1     2        1  4  2     2     3     2  2  2  1     2  3  1  2  3     2|     35  2.0|
      Acinus, Hyperplasia, Focal           | 2  1     3        2                                2           1  2  3   |     15  1.9|
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  75                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 1| 1| 3| 3| 3| 1| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|            |
                                           | 1| 1| 3| 3| 0| 9| 0| 0| 0| 2| 6| 1| 1| 1| 1| 1| 1| 0| 0| 0| 3| 3| 3| 1| 1|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|      A     |
    25 MG/KG                               | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Edema                                |                                                                          |      1  2.0|
      Ulcer                                |                                                                          |      2  2.5|
      Epithelium, Hyperplasia              |                                                                1         |      3  1.7|
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Erosion                              |                                                                          |      1  3.0|
      Ulcer                                |                                                                          |      2  2.0|
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Blood Vessel                            |                                  +                                       |   1        |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cardiomyopathy                       | 2  2  2  1  1     2  2  2  2  2  1  1  2  1  1  1     2  1  1  2  2  1  1|     47  1.4|
      Thrombosis                           | 1                                                                        |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Accessory Adrenal Cortical Nodule    |    1  1           1  2  1     2  2              1           1  1  1  3  2|     28  1.3|
      Degeneration, Fatty                  |             2        2     2                 1                           |      7  1.7|
      Hyperplasia, Focal                   |    1           1           2        2                                    |      5  1.6|
      Hypertrophy, Focal                   | 1           2        2     2                 2                           |      9  1.9|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          | 1                             2                                   2  2  2|      8  1.6|
      Thrombosis                           |                                                          3               |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |          2              2  2     2           3  2           2  2  1      |     17  1.8|
      Metaplasia, Hepatocyte               |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  76                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 1| 1| 3| 3| 3| 1| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|            |
                                           | 1| 1| 3| 3| 0| 9| 0| 0| 0| 2| 6| 1| 1| 1| 1| 1| 1| 0| 0| 0| 3| 3| 3| 1| 1|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|      A     |
    25 MG/KG                               | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ENDOCRINE SYSTEM - cont                   |                                                                          |            |
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Pars Distalis, Angiectasis           |       1     4        2  2  3        2                 1  2              2|     16  2.1|
      Pars Distalis, Cyst                  |          2                                                  2            |      6  1.8|
      Pars Distalis, Hyperplasia, Focal    |                                  2     2     2                    2  2   |      9  1.8|
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      C-Cell, Hyperplasia                  |                         2              2  2                              |      9  1.8|
      Follicle, Cyst                       |                1                                      1                  |      4  1.0|
      Follicular Cell, Hyperplasia         |                                                    3                     |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Peritoneum                              |                +                                                         |   1        |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Coagulating Gland                       |                                  +                                       |   2        |
      Inflammation                         |                                                                          |      1  4.0|
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation, Chronic                |                                     2                                   3|      4  2.0|
                                           |__________________________________________________________________________|____________|
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation, Chronic                |          2  2  1        2  2  2  2           2           1  2           2|     27  2.0|
                                           |__________________________________________________________________________|____________|
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Bilateral, Germinal Epithelium,      |                                                                          |            |
          Atrophy                          |                                                                          |      2  3.0|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  77                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 1| 1| 3| 3| 3| 1| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|            |
                                           | 1| 1| 3| 3| 0| 9| 0| 0| 0| 2| 6| 1| 1| 1| 1| 1| 1| 0| 0| 0| 3| 3| 3| 1| 1|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|      A     |
    25 MG/KG                               | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |                2                                                         |      4  2.5|
                                           |__________________________________________________________________________|____________|
   Lymph Node                              | +  +     +           +                                               +   |  10        |
      Deep Cervical, Hyperplasia, Lymphoid |                                                                          |      1  4.0|
      Mediastinal, Hyperplasia, Lymphoid   | 2  2     4           2                                               2   |     10  2.2|
      Pancreatic, Hyperplasia, Lymphoid    | 2                                                                        |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|            |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia, Lymphoid                | 2  2     2                                                               |      4  2.0|
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Fibrosis                             |    2                       2                                             |      2  2.0|
      Hematopoietic Cell Proliferation     |    1  2        4        1  1     1           1  1                       1|     17  1.5|
      Capsule, Fibrosis                    |                   2        2           1     2  2  1                     |     12  1.5|
      Lymphoid Follicle, Hyperplasia       |             2                                                            |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 | 2        2  2           2  2  1                       2     1           2|     20  1.8|
      Hyperplasia                          |                                                                      1   |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation, Chronic                |                                              3        2                  |      5  2.6|
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  78                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 1| 1| 3| 3| 3| 1| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|            |
                                           | 1| 1| 3| 3| 0| 9| 0| 0| 0| 2| 6| 1| 1| 1| 1| 1| 1| 0| 0| 0| 3| 3| 3| 1| 1|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|      A     |
    25 MG/KG                               | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Compression                          |    2        2           2  4                                             |      9  2.2|
                                           |__________________________________________________________________________|____________|
   Peripheral Nerve                        |                                                                          |   4        |
                                           |__________________________________________________________________________|____________|
   Spinal Cord                             |                                                                          |   3        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Congestion                           |             2                                                            |      3  1.3|
      Foreign Body                         |                                                                          |      1  2.0|
      Infiltration Cellular, Histiocyte    | 1     1  1           2  2  1  2  1  1  1  1     1  1  1  1  1  1     1  1|     36  1.1|
      Inflammation, Chronic                |    1  1  1     1  1  2  1     1  1  1  1  1     1     1  1     1     1   |     36  1.0|
      Necrosis                             |                                                                          |      1  3.0|
      Alveolar Epithelium, Hyperplasia     | 3                                                                        |      4  2.3|
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Foreign Body                         | 2        1              1           2  2           2        2     1      |     12  1.7|
      Inflammation, Chronic                | 2        2  2        2  2           1  2        1  2        2     1      |     20  1.7|
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation                         |                                                                          |      1  2.0|
      Perforation                          |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Cataract                             |                                                                          |      4  2.8|
      Inflammation, Chronic                |                                                                          |      1  2.0|
      Retina, Degeneration                 |                                                                          |      4  3.5|
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  79                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 1| 1| 3| 3| 3| 1| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|            |
                                           | 1| 1| 3| 3| 0| 9| 0| 0| 0| 2| 6| 1| 1| 1| 1| 1| 1| 0| 0| 0| 3| 3| 3| 1| 1|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|      A     |
    25 MG/KG                               | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 SPECIAL SENSES SYSTEM - cont              |                                                                          |            |
      Hyperplasia                          |                                                                          |      1  2.0|
      Inflammation, Chronic                |                                                                          |      1  1.0|
      Pigmentation                         |             2                                                            |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Zymbal's Gland                          |                                                                          |   1        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 |                                                                   3      |      1  3.0|
      Nephropathy                          | 1  1  1  1           1  1  2     1     1     1  2  1  1  2  1  2  1  1  2|     33  1.3|
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |                                                                          |      1  3.0|
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  80                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 5| 7| 7| 6| 1| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 5| 7|             
                             DAY ON TEST   | 3| 2| 0| 3| 3| 5| 9| 6| 3| 3| 3| 3| 3| 3| 1| 2| 2| 3| 3| 0| 3| 3| 3| 9| 3|             
                                           | 0| 9| 8| 3| 3| 2| 1| 3| 3| 3| 3| 3| 1| 1| 5| 9| 9| 0| 1| 3| 1| 1| 1| 7| 2|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    50 MG/KG                               | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Perforation                          |       3           4                                                      |             
                                           |__________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +|             
      Inflammation, Chronic                |    2                                                                     |             
                                           |__________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +|             
      Hyperplasia, Lymphoid                |                                     3                                    |             
                                           |__________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Basophilic Focus                     | X           X           2                                               X|             
      Clear Cell Focus                     |             X           X  X     X     X        X                       X|             
      Degeneration, Cystic                 |                                                                   2      |             
      Eosinophilic Focus                   |                                        X           X                     |             
      Hepatodiaphragmatic Nodule           |                            2                 2                           |             
      Inflammation, Chronic                |                                                          1               |             
      Bile Duct, Hyperplasia               |          2  2           2  3  2  2  2  2  2  1  1  1  2  2  3  2  2  2  2|             
      Centrilobular, Necrosis              |                                                                          |             
      Hepatocyte, Vacuolization Cytoplasmic|    3                                                                     |             
      Serosa, Inflammation                 |    2                                                                     |             
                                           |__________________________________________________________________________|             
   Mesentery                               |                                                    +           +  +      |             
      Accessory Spleen                     |                                                                          |             
      Fat, Necrosis                        |                                                    2              2      |             
                                           |__________________________________________________________________________|             
   Oral Mucosa                             |                +                          +                              |             
      Pharyngeal, Cyst                     |                                                                          |             
      Pharyngeal, Inflammation             |                                                                          |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  81                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 5| 7| 7| 6| 1| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 5| 7|             
                             DAY ON TEST   | 3| 2| 0| 3| 3| 5| 9| 6| 3| 3| 3| 3| 3| 3| 1| 2| 2| 3| 3| 0| 3| 3| 3| 9| 3|             
                                           | 0| 9| 8| 3| 3| 2| 1| 3| 3| 3| 3| 3| 1| 1| 5| 9| 9| 0| 1| 3| 1| 1| 1| 7| 2|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    50 MG/KG                               | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM - cont                  |                                                                          |             
                                           |__________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy, Focal                       |                                                                          |             
      Atrophy                              |    2     1  2  1     1  1  1  1  1  2  2  4  2  3              2  1      |             
      Cyst                                 |          2                                                               |             
      Inflammation, Chronic                |       2                                                                  |             
      Acinus, Hyperplasia, Focal           |    2           1           3        2  2                          1      |             
                                           |__________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          | 2                                                                        |             
                                           |__________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Edema                                |                                                                          |             
      Erosion                              |                                                                   1      |             
      Ulcer                                |                                                                          |             
      Epithelium, Hyperplasia              |                                                                   1      |             
                                           |__________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Edema                                |    2                                                                     |             
      Erosion                              |                2                                                         |             
      Ulcer                                |    4                                                                     |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Blood Vessel                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       | 2  1  1  4  2  1  1  2  2  2  3  2  2  1  1  2  2  2  2  2  1  1  1      |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Accessory Adrenal Cortical Nodule    | 1        1  1  2           1  1     2  2  1  1  1     1  1     1  2     1|             
      Degeneration, Fatty                  |    2                                                                     |             
      Hyperplasia, Focal                   |             1                    1              1                        |             
      Hypertrophy, Focal                   | 2                                                  2                     |             
      Necrosis                             |    3                                                                     |             
                                           |__________________________________________________________________________|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  82                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 5| 7| 7| 6| 1| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 5| 7|             
                             DAY ON TEST   | 3| 2| 0| 3| 3| 5| 9| 6| 3| 3| 3| 3| 3| 3| 1| 2| 2| 3| 3| 0| 3| 3| 3| 9| 3|             
                                           | 0| 9| 8| 3| 3| 2| 1| 3| 3| 3| 3| 3| 1| 1| 5| 9| 9| 0| 1| 3| 1| 1| 1| 7| 2|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    50 MG/KG                               | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ENDOCRINE SYSTEM - cont                   |                                                                          |             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |          2                                               2               |             
                                           |__________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |    2           1     1  1     1  1     1  1     2           3  2  1     1|             
                                           |__________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Pars Distalis, Angiectasis           |    2  2        2        2     2                 2  2     2           3   |             
      Pars Distalis, Cyst                  |    2                          2                                   1      |             
      Pars Distalis, Hyperplasia, Focal    |          2  2        2     3     3  2                          2         |             
                                           |__________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Ultimobranchial Cyst                 |                                                          2  1            |             
      C-Cell, Hyperplasia                  |                2                                      2                  |             
      Follicle, Cyst                       |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |__________________________________________________________________________|             
   Peritoneum                              |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                          |             
      Inflammation, Chronic                |                                                                          |             
                                           |__________________________________________________________________________|             
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic                | 2  4     1     1     2  2     2     2              3     2  1     2      |             
                                           |__________________________________________________________________________|             
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Germinal Epithelium, Atrophy         |    3                                                                     |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  83                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 5| 7| 7| 6| 1| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 5| 7|             
                             DAY ON TEST   | 3| 2| 0| 3| 3| 5| 9| 6| 3| 3| 3| 3| 3| 3| 1| 2| 2| 3| 3| 0| 3| 3| 3| 9| 3|             
                                           | 0| 9| 8| 3| 3| 2| 1| 3| 3| 3| 3| 3| 1| 1| 5| 9| 9| 0| 1| 3| 1| 1| 1| 7| 2|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    50 MG/KG                               | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                2     3  2                 3           3                  |             
                                           |__________________________________________________________________________|             
   Lymph Node                              | +  +  +        +        +                    +        +              +  +|             
      Mediastinal, Hyperplasia, Lymphoid   | 2     2                 2                    2        2              2  2|             
      Pancreatic, Hyperplasia, Lymphoid    |                                                                          |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mandibular                  | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|             
                                           |__________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Lymphoid                |                         2           4           2     2                 2|             
                                           |__________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Fibrosis                             |                                                                2         |             
      Hematopoietic Cell Proliferation     | 2              2     2        1           4  2     2     2        1      |             
      Hemorrhage                           |                            2                                             |             
      Capsule, Fibrosis                    | 2        2  2  2     2  2        2  2  2  1        2  2  2  2  2  1     2|             
                                           |__________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 | 2        1           2  3     2     2              1     2           3   |             
                                           |__________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperkeratosis                       |                                                          2               |             
      Inflammation, Chronic                |                                        2                                 |             
      Subcutaneous Tissue, Mineralization  |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Skeletal Muscle                         |                                                                   +      |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  84                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 5| 7| 7| 6| 1| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 5| 7|             
                             DAY ON TEST   | 3| 2| 0| 3| 3| 5| 9| 6| 3| 3| 3| 3| 3| 3| 1| 2| 2| 3| 3| 0| 3| 3| 3| 9| 3|             
                                           | 0| 9| 8| 3| 3| 2| 1| 3| 3| 3| 3| 3| 1| 1| 5| 9| 9| 0| 1| 3| 1| 1| 1| 7| 2|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    50 MG/KG                               | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 NERVOUS SYSTEM - cont                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Compression                          |                                                    3     2           1   |             
                                           |__________________________________________________________________________|             
   Peripheral Nerve                        |                               +                             +     +     +|             
                                           |__________________________________________________________________________|             
   Spinal Cord                             |                               +                             +     +     +|             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                   2                          2     2     1           2   |             
      Foreign Body                         |       2           2                                                      |             
      Infiltration Cellular, Histiocyte    | 1        1     1                             1  1     1  1  1     1      |             
      Inflammation, Chronic                |          1  1     3  1  1     1  1     1        1     1  1  1  1  1  1  1|             
      Inflammation, Suppurative            |                                                                          |             
      Necrosis                             |       2                                                                  |             
      Alveolar Epithelium, Hyperplasia     |                2        2  3                 2                           |             
                                           |__________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |                         2              2              2                 1|             
      Inflammation, Chronic                |                         2              2  2        2  2        1        2|             
                                           |__________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |       2                                                                  |             
      Necrosis                             |       2                                                                  |             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Eye                                     | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +|             
      Cataract                             |                               3                             3            |             
      Inflammation, Chronic                |                                           4                              |             
      Phthisis Bulbi                       |                                                                         X|             
      Anterior Chamber, Inflammation, Acute|                2                                                         |             
      Retina, Degeneration                 |                               4                             4            |             
                                           |__________________________________________________________________________|             
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                                                          |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  85                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 5| 7| 7| 6| 1| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 5| 7|             
                             DAY ON TEST   | 3| 2| 0| 3| 3| 5| 9| 6| 3| 3| 3| 3| 3| 3| 1| 2| 2| 3| 3| 0| 3| 3| 3| 9| 3|             
                                           | 0| 9| 8| 3| 3| 2| 1| 3| 3| 3| 3| 3| 1| 1| 5| 9| 9| 0| 1| 3| 1| 1| 1| 7| 2|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    50 MG/KG                               | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 SPECIAL SENSES SYSTEM - cont              |                                                                          |             
      Inflammation, Chronic                |                      1     1                          1                  |             
      Pigmentation                         |       2                                                                  |             
                                           |__________________________________________________________________________|             
   Zymbal's Gland                          |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hydronephrosis                       |                                                                          |             
      Nephropathy                          |    2     1  1  1     1  2  1  1  1  1  1  1  1     1  2  3              1|             
                                           |__________________________________________________________________________|             
   Urethra                                 |                                                                          |             
      Inflammation                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                                                          |             
      Inflammation, Chronic                |                                                                          |             
 __________________________________________|__________________________________________________________________________              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  86                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 6| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 5| 7| 4| 7| 6| 4| 7| 7| 5| 7| 7|            |
                             DAY ON TEST   | 8| 3| 5| 3| 3| 2| 2| 2| 3| 2| 6| 2| 2| 1| 7| 3| 6| 3| 9| 0| 3| 3| 0| 3| 3|            |
                                           | 7| 2| 8| 1| 1| 9| 9| 9| 1| 6| 6| 6| 9| 7| 6| 2| 5| 0| 2| 1| 2| 2| 4| 3| 3|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2|      A     |
    50 MG/KG                               | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Perforation                          |                                                                          |      2  3.5|
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation                         | 2                                                                        |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
      Inflammation, Chronic                |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Hyperplasia, Lymphoid                |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Basophilic Focus                     |             X           X                                                |      6  2.0|
      Clear Cell Focus                     |    X        X  X           2                                   X         |     12  2.0|
      Degeneration, Cystic                 |                                                                          |      1  2.0|
      Eosinophilic Focus                   |                                              X                           |      3     |
      Hepatodiaphragmatic Nodule           |                                        2                    2            |      4  2.0|
      Inflammation, Chronic                |                                                                          |      1  1.0|
      Bile Duct, Hyperplasia               | 2  2     2  2  2     2  2     2     2  2  2  2     2  2     2  2     2  2|     37  2.0|
      Centrilobular, Necrosis              |       2                                               2                  |      2  2.0|
      Hepatocyte, Vacuolization Cytoplasmic|                                                          1        3      |      3  2.3|
      Serosa, Inflammation                 |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Mesentery                               | +  +  +  +                    +  +  +  +                             +   |  12        |
      Accessory Spleen                     |          2                                                               |      1  2.0|
      Fat, Necrosis                        |    3     3                    3        2                             3   |      7  2.6|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  87                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 6| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 5| 7| 4| 7| 6| 4| 7| 7| 5| 7| 7|            |
                             DAY ON TEST   | 8| 3| 5| 3| 3| 2| 2| 2| 3| 2| 6| 2| 2| 1| 7| 3| 6| 3| 9| 0| 3| 3| 0| 3| 3|            |
                                           | 7| 2| 8| 1| 1| 9| 9| 9| 1| 6| 6| 6| 9| 7| 6| 2| 5| 0| 2| 1| 2| 2| 4| 3| 3|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2|      A     |
    50 MG/KG                               | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Oral Mucosa                             |                                           +           +                  |   4        |
      Pharyngeal, Cyst                     |                                           4           4                  |      2  4.0|
      Pharyngeal, Inflammation             |                                           2           3                  |      2  2.5|
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Atrophy, Focal                       |                                                             1            |      1  1.0|
      Atrophy                              | 2     4  1  2  2  1  1  2  2  2  2  3  1  4  2     2                     |     32  1.9|
      Cyst                                 |                                                                          |      1  2.0|
      Inflammation, Chronic                |                                                                          |      1  2.0|
      Acinus, Hyperplasia, Focal           | 1  2                             2                 1                 3  2|     12  1.8|
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Edema                                |                                                          1        4      |      2  2.5|
      Erosion                              |                                                                          |      1  1.0|
      Ulcer                                |                                                 2                 3      |      2  2.5|
      Epithelium, Hyperplasia              |                                                          1        2      |      3  1.3|
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Edema                                |                                                 2                        |      2  2.0|
      Erosion                              |                                                                          |      1  2.0|
      Ulcer                                |                                                                          |      1  4.0|
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Blood Vessel                            | +                                                                        |   1        |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cardiomyopathy                       | 2  3  1  1  2  1  2  1  2  2  2  1     1  1  1     1  2     2  2  1  2  2|     45  1.7|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  88                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 6| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 5| 7| 4| 7| 6| 4| 7| 7| 5| 7| 7|            |
                             DAY ON TEST   | 8| 3| 5| 3| 3| 2| 2| 2| 3| 2| 6| 2| 2| 1| 7| 3| 6| 3| 9| 0| 3| 3| 0| 3| 3|            |
                                           | 7| 2| 8| 1| 1| 9| 9| 9| 1| 6| 6| 6| 9| 7| 6| 2| 5| 0| 2| 1| 2| 2| 4| 3| 3|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2|      A     |
    50 MG/KG                               | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ENDOCRINE SYSTEM - cont                   |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Accessory Adrenal Cortical Nodule    |             1  1                       1  1  1  1     1        2     1   |     25  1.2|
      Degeneration, Fatty                  |                1                                                         |      2  1.5|
      Hyperplasia, Focal                   |                            2           1                                 |      5  1.2|
      Hypertrophy, Focal                   | 2  2     1     3                    2                          2         |      8  2.0|
      Necrosis                             |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |                                                                          |      2  2.0|
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          | 2        2  2  1     1     2                 1     1  1  2  1  2        1|     26  1.4|
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Pars Distalis, Angiectasis           |    2     2  3  2              3           2                              |     15  2.2|
      Pars Distalis, Cyst                  |                      2                 3                                 |      5  2.0|
      Pars Distalis, Hyperplasia, Focal    | 2  3              2     2                                      3        2|     13  2.3|
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Ultimobranchial Cyst                 |                                                                          |      2  1.5|
      C-Cell, Hyperplasia                  |                                           3                    2         |      4  2.3|
      Follicle, Cyst                       |                         1                                                |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Peritoneum                              |       +                          +                                       |   2        |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  89                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 6| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 5| 7| 4| 7| 6| 4| 7| 7| 5| 7| 7|            |
                             DAY ON TEST   | 8| 3| 5| 3| 3| 2| 2| 2| 3| 2| 6| 2| 2| 1| 7| 3| 6| 3| 9| 0| 3| 3| 0| 3| 3|            |
                                           | 7| 2| 8| 1| 1| 9| 9| 9| 1| 6| 6| 6| 9| 7| 6| 2| 5| 0| 2| 1| 2| 2| 4| 3| 3|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2|      A     |
    50 MG/KG                               | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM - cont                     |                                                                          |            |
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 | 2           1     2                                                      |      3  1.7|
      Inflammation, Chronic                |    2        1     2                                2                     |      4  1.8|
                                           |__________________________________________________________________________|____________|
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation, Chronic                |          1  2     2        2  3           3     3  2     2  2  2  2  2  2|     26  2.1|
                                           |__________________________________________________________________________|____________|
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Germinal Epithelium, Atrophy         |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          | 2     3                          4           2                           |      9  2.7|
                                           |__________________________________________________________________________|____________|
   Lymph Node                              |    +     +     +        +        +  +                       +  +  +      |  18        |
      Mediastinal, Hyperplasia, Lymphoid   |    2     2     2        2        2  2                       2  2         |     15  2.0|
      Pancreatic, Hyperplasia, Lymphoid    |                                                                   2      |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|            |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia, Lymphoid                |    2     2                                      1                 2      |      9  2.1|
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Fibrosis                             |                                                       2                  |      2  2.0|
      Hematopoietic Cell Proliferation     | 2     3              1     1     2     1     1     1        1     2  1   |     20  1.7|
      Hemorrhage                           |                                                       3                  |      2  2.5|
      Capsule, Fibrosis                    |    2        1  2  1  2  2  2              2                 1  2  2  1  2|     30  1.8|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                             Page  90                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 6| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 5| 7| 4| 7| 6| 4| 7| 7| 5| 7| 7|            |
                             DAY ON TEST   | 8| 3| 5| 3| 3| 2| 2| 2| 3| 2| 6| 2| 2| 1| 7| 3| 6| 3| 9| 0| 3| 3| 0| 3| 3|            |
                                           | 7| 2| 8| 1| 1| 9| 9| 9| 1| 6| 6| 6| 9| 7| 6| 2| 5| 0| 2| 1| 2| 2| 4| 3| 3|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2|      A     |
    50 MG/KG                               | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 | 2  2     2  2  1     2        2     2  2                          2  1   |     20  1.9|
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperkeratosis                       |                                                                          |      1  2.0|
      Inflammation, Chronic                |                                                                          |      1  2.0|
      Subcutaneous Tissue, Mineralization  |                                                                         2|      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Skeletal Muscle                         |                            +     +                       +               |   4        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Compression                          |             3                          2                          3      |      6  2.3|
                                           |__________________________________________________________________________|____________|
   Peripheral Nerve                        |                            +                             +               |   6        |
                                           |__________________________________________________________________________|____________|
   Spinal Cord                             |                            +                             +               |   6        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Congestion                           |                               2           2                              |      7  1.9|
      Foreign Body                         |                                                                          |      2  2.0|
      Infiltration Cellular, Histiocyte    | 1  1  1  1  1  1  1  1  1        1  1     1  1        1     1  1        1|     26  1.0|
      Inflammation, Chronic                | 1  1     1     1  1     1  1        1  1     1           4  1        1  1|     30  1.2|
      Inflammation, Suppurative            |                                                       3                  |      1  3.0|
      Necrosis                             |                                                                          |      1  2.0|
      Alveolar Epithelium, Hyperplasia     | 1                                            3                           |      6  2.2|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  91                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 6| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 5| 7| 4| 7| 6| 4| 7| 7| 5| 7| 7|            |
                             DAY ON TEST   | 8| 3| 5| 3| 3| 2| 2| 2| 3| 2| 6| 2| 2| 1| 7| 3| 6| 3| 9| 0| 3| 3| 0| 3| 3|            |
                                           | 7| 2| 8| 1| 1| 9| 9| 9| 1| 6| 6| 6| 9| 7| 6| 2| 5| 0| 2| 1| 2| 2| 4| 3| 3|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2|      A     |
    50 MG/KG                               | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 RESPIRATORY SYSTEM - cont                 |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Foreign Body                         |                      1                    2                    2         |      7  1.7|
      Inflammation, Chronic                | 2     2              1           2        2     2        3     2         |     15  1.9|
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Foreign Body                         |                                                                          |      1  2.0|
      Necrosis                             |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Cataract                             |                                                                      2   |      3  2.7|
      Inflammation, Chronic                |                                                                          |      1  4.0|
      Phthisis Bulbi                       |                                                                          |      1     |
      Anterior Chamber, Inflammation, Acute|                                                                          |      1  2.0|
      Retina, Degeneration                 |                                                                      3   |      3  3.7|
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |                         2                                                |      1  2.0|
      Inflammation, Chronic                |                1                                                         |      4  1.0|
      Pigmentation                         |                            2                                      2      |      3  2.0|
                                           |__________________________________________________________________________|____________|
   Zymbal's Gland                          |                                  +                                       |   1        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hydronephrosis                       |                                  2              3                        |      2  2.5|
      Nephropathy                          | 1  1        2  1     1  2  1           1  1  1  1  1  1     1     3  1  2|     34  1.3|
                                           |__________________________________________________________________________|____________|
   Urethra                                 |                                                 +                        |   1        |
      Inflammation                         |                                                 3                        |      1  3.0|
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  92                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05    
Route: GAVAGE                                                                                                     Time: 10:12:13    
 __________________________________________________________________________________________________________________________________ 
                                           | 6| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 5| 7| 4| 7| 6| 4| 7| 7| 5| 7| 7|            |
                             DAY ON TEST   | 8| 3| 5| 3| 3| 2| 2| 2| 3| 2| 6| 2| 2| 1| 7| 3| 6| 3| 9| 0| 3| 3| 0| 3| 3|            |
                                           | 7| 2| 8| 1| 1| 9| 9| 9| 1| 6| 6| 6| 9| 7| 6| 2| 5| 0| 2| 1| 2| 2| 4| 3| 3|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2|      A     |
    50 MG/KG                               | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 URINARY SYSTEM - cont                     |                                                                          |            |
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Hemorrhage                           |                                                 2                        |      1  2.0|
      Inflammation, Chronic                |                                                 3                        |      1  3.0|
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  93                                                               
                                                                                                                                   
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                             ----------              END OF REPORT             ----------                                           
                             ------------------------------------------------------------                                           
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.