Share This:
https://ntp.niehs.nih.gov/go/20997

TDMS Study 91005-08 Pathology Tables

NTP Experiment-Test: 91005-08  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 04/26/05
Route: GAVAGE                                                                                                     Time: 09:25:25

                                                         FINAL #1 MICE




       Facility:  Southern Research Institute

       Chemical CAS #:  7220-79-3

       Lock Date:  09/05/03

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All


































a  Number of animals examined microscopically at site and number of animals with lesion

                                                              Page   1


NTP Experiment-Test: 91005-08  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 04/26/05  
Route: GAVAGE                                                                                                     Time: 09:25:25  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/KG      2.5          12.5         25                                        
                                                                MG/KG        MG/KG        MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially in Study                          50           50           50           50                                     
  Early Deaths                                                                                                                      
    Natural Death                                      9            5            2            6                                     
    Moribund Sacrifice                                 7            4            5            1                                     
    Accidently Killed                                  1                                                                            
    Dosing Accident                                                 1            1                                                  
  Survivors                                                                                                                         
    Terminal Sacrifice                                29           40           41           41                                     
    Natural Death                                      2                         1            2                                     
    Moribund Sacrifice                                 2                                                                            
                                                                                                                                    
  Animals Examined Microscopically                    50           50           50           50                                     
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Esophagus                                          (50)         (50)         (50)         (50)                                   
      Fibrosarcoma, Metastatic, Skin                                1 (2%)                                                          
   Gallbladder                                        (42)         (45)         (47)         (44)                                   
   Intestine Large, Cecum                             (43)         (46)         (47)         (44)                                   
   Intestine Small, Duodenum                          (43)         (45)         (47)         (42)                                   
      Carcinoma                                        1 (2%)                                                                       
   Intestine Small, Jejunum                           (43)         (45)         (47)         (43)                                   
      Adenoma                                                       1 (2%)                                                          
   Intestine Small, Ileum                             (43)         (44)         (47)         (43)                                   
   Liver                                              (50)         (50)         (50)         (50)                                   
      Fibrosarcoma, Metastatic, Skin                                1 (2%)                                                          
      Hemangiosarcoma                                                            2 (4%)                                             
      Hepatoblastoma                                                             1 (2%)                                             
      Hepatocellular Carcinoma                         5 (10%)      4 (8%)                    3 (6%)                                
      Hepatocellular Adenoma                          11 (22%)     15 (30%)     13 (26%)      9 (18%)                               
      Hepatocellular Adenoma, Multiple                 4 (8%)       4 (8%)       9 (18%)      4 (8%)                                
      Histiocytic Sarcoma                              2 (4%)                                                                       
      Serosa, Fibrosarcoma, Metastatic, Skin                                                  1 (2%)                                
   Mesentery                                          (18)         (14)         (15)         (13)                                   
      Fibrosarcoma                                                                            1 (8%)                                
      Fibrosarcoma, Metastatic, Skin                                1 (7%)       1 (7%)       1 (8%)                                
      Histiocytic Sarcoma                              1 (6%)                                 1 (8%)                                
      Leiomyosarcoma, Metastatic, Uterus                                                      1 (8%)                                
      Rhabdomyosarcoma, Metastatic, Skin                                         1 (7%)                                             
      Rhabdomyosarcoma, Metastatic, Uncertain                                                                                       
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   2                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 04/26/05  
Route: GAVAGE                                                                                                     Time: 09:25:25  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/KG      2.5          12.5         25                                        
                                                                MG/KG        MG/KG        MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - cont                                                                                                            
          Primary Site                                 1 (6%)                                                                       
   Pancreas                                           (46)         (46)         (49)         (49)                                   
      Fibrosarcoma, Metastatic, Skin                                             1 (2%)                                             
      Histiocytic Sarcoma                                                                     1 (2%)                                
   Salivary Glands                                    (50)         (50)         (50)         (49)                                   
   Stomach, Forestomach                               (50)         (50)         (50)         (49)                                   
      Squamous Cell Papilloma                                       1 (2%)       1 (2%)       1 (2%)                                
   Stomach, Glandular                                 (45)         (47)         (50)         (46)                                   
      Carcinoma                                                                  1 (2%)                                             
   Tooth                                              (2)          (10)         (3)          (3)                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (50)         (50)         (50)         (50)                                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                        1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (50)         (50)         (50)         (50)                                   
      Adenoma                                                                    1 (2%)                                             
      Histiocytic Sarcoma                              1 (2%)                                                                       
      Capsule, Fibrosarcoma, Metastatic, Skin                       1 (2%)                                                          
   Adrenal Medulla                                    (50)         (50)         (49)         (50)                                   
      Pheochromocytoma Benign                          1 (2%)       1 (2%)       2 (4%)                                             
   Islets, Pancreatic                                 (46)         (47)         (49)         (48)                                   
      Adenoma                                          1 (2%)                                 1 (2%)                                
      Carcinoma                                                                               1 (2%)                                
   Pituitary Gland                                    (46)         (49)         (48)         (47)                                   
      Pars Distalis, Adenoma                           1 (2%)       7 (14%)      5 (10%)      3 (6%)                                
      Pars Intermedia, Adenoma                         1 (2%)                                                                       
   Thyroid Gland                                      (46)         (50)         (49)         (47)                                   
      Follicular Cell, Adenoma                         1 (2%)       1 (2%)                    1 (2%)                                
      Follicular Cell, Carcinoma                                                              1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   3                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 04/26/05  
Route: GAVAGE                                                                                                     Time: 09:25:25  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/KG      2.5          12.5         25                                        
                                                                MG/KG        MG/KG        MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   Peritoneum                                                      (1)                                                              
   Tissue NOS                                         (3)                                    (3)                                    
      Abdominal, Histiocytic Sarcoma                                                          1 (33%)                               
      Thoracic, Histiocytic Sarcoma                    1 (33%)                                                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Clitoral Gland                                     (48)         (50)         (46)         (48)                                   
   Ovary                                              (47)         (48)         (49)         (48)                                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                        1 (2%)                                                                       
      Cystadenoma                                      2 (4%)       3 (6%)       3 (6%)       2 (4%)                                
      Fibrosarcoma, Metastatic, Skin                                1 (2%)                                                          
      Granulosa-Theca Tumor Benign                                                            1 (2%)                                
      Hemangiosarcoma                                  1 (2%)                                                                       
      Histiocytic Sarcoma                              1 (2%)                                 1 (2%)                                
      Luteoma                                                                    1 (2%)                                             
   Oviduct                                                         (1)                                                              
   Uterus                                             (50)         (50)         (50)         (50)                                   
      Adenoma                                          1 (2%)       1 (2%)                                                          
      Hemangioma                                                    1 (2%)                    1 (2%)                                
      Hemangiosarcoma                                  1 (2%)                                                                       
      Histiocytic Sarcoma                              1 (2%)                                                                       
      Leiomyoma                                        1 (2%)                                                                       
      Leiomyosarcoma                                                                          1 (2%)                                
      Endometrium, Polyp Stromal                                    2 (4%)       2 (4%)                                             
   Vagina                                             (1)                                                                           
      Squamous Cell Carcinoma                          1 (100%)                                                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (50)         (50)         (49)         (50)                                   
      Hemangiosarcoma                                                            1 (2%)                                             
      Histiocytic Sarcoma                              1 (2%)                                                                       
   Lymph Node                                         (7)          (5)          (10)         (10)                                   
      Iliac, Fibrosarcoma, Metastatic, Skin                         1 (20%)                                                         
      Iliac, Histiocytic Sarcoma                       1 (14%)                                                                      
      Inguinal, Fibrosarcoma, Metastatic, Skin         1 (14%)                                                                      
      Inguinal, Histiocytic Sarcoma                    1 (14%)                                                                      
      Mediastinal, Histiocytic Sarcoma                 1 (14%)                                1 (10%)                               
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   4                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 04/26/05  
Route: GAVAGE                                                                                                     Time: 09:25:25  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/KG      2.5          12.5         25                                        
                                                                MG/KG        MG/KG        MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
HEMATOPOIETIC SYSTEM - cont                                                                                                         
      Pancreatic, Fibrosarcoma, Metastatic, Skin                    1 (20%)                                                         
      Pancreatic, Histiocytic Sarcoma                  1 (14%)                                                                      
      Pancreatic, Leiomyosarcoma, Metastatic,                                                                                       
          Uterus                                                                              1 (10%)                               
      Popliteal, Rhabdomyosarcoma, Metastatic,                                                                                      
           Skeletal Muscle                                                       1 (10%)                                            
   Lymph Node, Mandibular                             (48)         (49)         (48)         (46)                                   
      Histiocytic Sarcoma                              1 (2%)                                                                       
   Lymph Node, Mesenteric                             (46)         (49)         (49)         (48)                                   
      Histiocytic Sarcoma                              1 (2%)                                 1 (2%)                                
   Spleen                                             (47)         (47)         (49)         (50)                                   
      Hemangiosarcoma                                                            1 (2%)                                             
      Histiocytic Sarcoma                              1 (2%)                                                                       
   Thymus                                             (43)         (47)         (44)         (49)                                   
      Histiocytic Sarcoma                              1 (2%)                                 1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (50)         (50)         (50)         (50)                                   
      Carcinoma                                        2 (4%)       1 (2%)                    2 (4%)                                
   Skin                                               (50)         (50)         (50)         (50)                                   
      Basal Cell Carcinoma                             1 (2%)                                                                       
      Subcutaneous Tissue, Fibrosarcoma                3 (6%)       2 (4%)       2 (4%)       2 (4%)                                
      Subcutaneous Tissue, Hemangioma                  1 (2%)                                                                       
      Subcutaneous Tissue, Hemangiosarcoma             1 (2%)       1 (2%)       2 (4%)                                             
      Subcutaneous Tissue, Histiocytic Sarcoma         1 (2%)                                                                       
      Subcutaneous Tissue, Leiomyosarcoma,                                                                                          
          Metastatic, Uterus                                                                  1 (2%)                                
      Subcutaneous Tissue, Lipoma                                                1 (2%)                                             
      Subcutaneous Tissue, Rhabdomyosarcoma                                      1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (50)         (50)         (50)         (50)                                   
      Cranium, Osteosarcoma                            1 (2%)                                                                       
      Vertebra, Osteosarcoma                                        1 (2%)                                                          
   Skeletal Muscle                                    (1)          (4)          (3)          (3)                                    
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                        1 (100%)                                                                     
      Fibrosarcoma, Metastatic, Skin                                1 (25%)      1 (33%)      1 (33%)                               
      Hemangioma                                                                              1 (33%)                               
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   5                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 04/26/05  
Route: GAVAGE                                                                                                     Time: 09:25:25  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/KG      2.5          12.5         25                                        
                                                                MG/KG        MG/KG        MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
MUSCULOSKELETAL SYSTEM - cont                                                                                                       
      Leiomyosarcoma, Metastatic, Uterus                                                      1 (33%)                               
      Rhabdomyosarcoma                                              2 (50%)      2 (67%)                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (50)         (50)         (50)         (49)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (50)         (50)         (50)         (50)                                   
      Alveolar/Bronchiolar Adenoma                     1 (2%)       2 (4%)       3 (6%)       2 (4%)                                
      Alveolar/Bronchiolar Carcinoma                   4 (8%)                                 1 (2%)                                
      Alveolar/Bronchiolar Carcinoma, Multiple         1 (2%)                                                                       
      Carcinoma, Metastatic, Harderian Gland                                                  1 (2%)                                
      Fibrosarcoma, Metastatic, Mesentery                                                     1 (2%)                                
      Fibrosarcoma, Metastatic, Skin                   1 (2%)                    1 (2%)       1 (2%)                                
      Hepatocellular Carcinoma, Metastatic, Liver                   2 (4%)                                                          
      Histiocytic Sarcoma                              1 (2%)                                 1 (2%)                                
      Rhabdomyosarcoma, Metastatic, Skeletal Muscle                              1 (2%)                                             
      Squamous Cell Carcinoma, Metastatic, Vagina      1 (2%)                                                                       
      Mediastinum, Alveolar/Bronchiolar Carcinoma,                                                                                  
           Metastatic, Lung                            1 (2%)                                                                       
      Mediastinum, Fibrosarcoma, Metastatic, Skin                                1 (2%)                                             
      Mediastinum, Histiocytic Sarcoma                                                        1 (2%)                                
   Nose                                               (50)         (50)         (50)         (50)                                   
   Trachea                                            (49)         (50)         (50)         (49)                                   
      Carcinoma, Metastatic, Thyroid Gland                                                    1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Eye                                                (43)         (48)         (47)         (47)                                   
   Harderian Gland                                    (49)         (50)         (50)         (50)                                   
      Adenoma                                          4 (8%)       2 (4%)       7 (14%)      3 (6%)                                
      Carcinoma                                                     1 (2%)       2 (4%)       2 (4%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   6                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 04/26/05  
Route: GAVAGE                                                                                                     Time: 09:25:25  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/KG      2.5          12.5         25                                        
                                                                MG/KG        MG/KG        MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (48)         (50)         (49)         (50)                                   
      Histiocytic Sarcoma                              1 (2%)                                                                       
   Urinary Bladder                                    (45)         (47)         (49)         (48)                                   
      Hemangiosarcoma                                                                         1 (2%)                                
      Histiocytic Sarcoma                                                                     1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SYSTEMIC LESIONS                                                                                                                   
                                                                                                                                    
   Multiple Organs                                   *(50)        *(50)        *(50)        *(50)                                   
      Histiocytic Sarcoma                              2 (4%)                                 1 (2%)                                
      Lymphoma Malignant                               6 (12%)      4 (8%)       9 (18%)     12 (24%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
     *   Number of animals with any tissue examined microscopically                                                                 
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   7                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 04/26/05  
Route: GAVAGE                                                                                                     Time: 09:25:25  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/KG      2.5          12.5         25                                        
                                                                MG/KG        MG/KG        MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
TUMOR SUMMARY                                                                                                                       
                                                                                                                                    
   Total Animals with Primary Neoplasms (b)            38          36          40          37                                       
     Total Primary Neoplasms                           60          57          72          57                                       
                                                                                                                                    
   Total Animals with Benign Neoplasms                 25          29          32          22                                       
     Total Benign Neoplasms                            30          41          48          29                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms              25          15          18          24                                       
     Total Malignant Neoplasms                         30          16          24          28                                       
                                                                                                                                    
   Total Animals with Metastatic Neoplasms              5           4           5           5                                       
     Total Metastatic Neoplasm                          8          11           8          11                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms               1           1                                                               
   Uncertain Primary Site                                                                                                           
                                                                                                                                    
   Total Animals with Neoplasms Uncertain-                                                                                          
   Benign or Malignant                                                                                                              
     Total Uncertain Neoplasms                                                                                                      
                                                                                                                                    
                                                                                                                                    
____________________________________________________________________________________________________________________________________
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Primary tumors: all tumors except metastatic tumors                                                                              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   8                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 04/26/05  
Route: GAVAGE                                                                                                     Time: 09:25:25  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          0 MG/KG      2.5          12.5         25                                        
                                                                MG/KG        MG/KG        MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially in Study                          50           50           50           50                                     
  Early Deaths                                                                                                                      
    Natural Death                                     11            6            5            3                                     
    Moribund Sacrifice                                 4            4            7            4                                     
    Accidently Killed                                               1                                                               
    Dosing Accident                                                 1                         2                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                35           38           38           41                                     
                                                                                                                                    
  Animals Examined Microscopically                    50           50           50           50                                     
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Gallbladder                                        (40)         (44)         (45)         (44)                                   
      Histiocytic Sarcoma                                                        1 (2%)                                             
   Intestine Large, Cecum                             (45)         (46)         (46)         (47)                                   
      Carcinoma                                        1 (2%)                                                                       
   Intestine Small, Duodenum                          (42)         (46)         (46)         (46)                                   
      Adenoma                                                                    1 (2%)       2 (4%)                                
      Carcinoma                                                     1 (2%)                    1 (2%)                                
      Histiocytic Sarcoma                                                        1 (2%)                                             
   Intestine Small, Jejunum                           (45)         (45)         (47)         (47)                                   
      Adenoma                                          1 (2%)       1 (2%)       1 (2%)                                             
      Carcinoma                                                                  2 (4%)       2 (4%)                                
      Hepatocholangiocarcinoma, Metastatic, Liver                   1 (2%)                                                          
      Histiocytic Sarcoma                                                        1 (2%)                                             
   Intestine Small, Ileum                             (43)         (45)         (44)         (47)                                   
      Carcinoma                                                                               1 (2%)                                
   Liver                                              (50)         (50)         (50)         (49)                                   
      Carcinoma, Metastatic, Pancreas                                                         1 (2%)                                
      Cholangiocarcinoma                                            1 (2%)                                                          
      Hemangiosarcoma                                  2 (4%)       5 (10%)      3 (6%)       1 (2%)                                
      Hepatoblastoma                                   2 (4%)       2 (4%)       2 (4%)                                             
      Hepatocellular Carcinoma                        11 (22%)      9 (18%)      8 (16%)     11 (22%)                               
      Hepatocellular Carcinoma, Multiple               2 (4%)       1 (2%)       3 (6%)       3 (6%)                                
      Hepatocellular Adenoma                          14 (28%)     20 (40%)     14 (28%)     14 (29%)                               
      Hepatocellular Adenoma, Multiple                14 (28%)     13 (26%)     12 (24%)     13 (27%)                               
      Hepatocholangiocarcinoma                                      1 (2%)                                                          
      Histiocytic Sarcoma                                                        2 (4%)                                             
   Mesentery                                          (5)          (11)         (5)          (4)                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   9                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 04/26/05  
Route: GAVAGE                                                                                                     Time: 09:25:25  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          0 MG/KG      2.5          12.5         25                                        
                                                                MG/KG        MG/KG        MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - cont                                                                                                            
      Carcinoma, Metastatic, Pancreas                                                         1 (25%)                               
      Hemangiosarcoma                                               2 (18%)                                                         
      Hepatocellular Carcinoma, Metastatic, Liver      1 (20%)                                                                      
      Hepatocholangiocarcinoma, Metastatic, Liver                   1 (9%)                                                          
      Histiocytic Sarcoma                                                        1 (20%)                                            
   Pancreas                                           (48)         (50)         (49)         (48)                                   
      Hepatocholangiocarcinoma, Metastatic, Liver                   1 (2%)                                                          
      Acinus, Carcinoma                                                                       1 (2%)                                
   Stomach, Forestomach                               (50)         (50)         (50)         (48)                                   
      Squamous Cell Papilloma                                       1 (2%)       2 (4%)       2 (4%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (50)         (50)         (50)         (50)                                   
      Hepatocholangiocarcinoma, Metastatic, Liver                   1 (2%)                                                          
      Histiocytic Sarcoma                                                        1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (48)         (50)         (50)         (48)                                   
      Adenoma                                                       1 (2%)                                                          
      Histiocytic Sarcoma                                                        1 (2%)                                             
      Subcapsular, Adenoma                             5 (10%)      4 (8%)       2 (4%)       5 (10%)                               
   Adrenal Medulla                                    (48)         (50)         (50)         (48)                                   
      Histiocytic Sarcoma                                                        1 (2%)                                             
      Pheochromocytoma Benign                                                    1 (2%)       2 (4%)                                
   Islets, Pancreatic                                 (50)         (50)         (49)         (48)                                   
      Adenoma                                                       2 (4%)       2 (4%)       1 (2%)                                
      Histiocytic Sarcoma                                                        1 (2%)                                             
   Thyroid Gland                                      (50)         (50)         (49)         (48)                                   
      Follicular Cell, Adenoma                         3 (6%)       1 (2%)       3 (6%)       1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   Peritoneum                                                                   (1)                                                 
      Histiocytic Sarcoma                                                        1 (100%)                                           
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  10                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 04/26/05  
Route: GAVAGE                                                                                                     Time: 09:25:25  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          0 MG/KG      2.5          12.5         25                                        
                                                                MG/KG        MG/KG        MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Coagulating Gland                                                                         (1)                                    
   Epididymis                                         (50)         (50)         (49)         (49)                                   
      Carcinoma, Metastatic, Pancreas                                                         1 (2%)                                
   Seminal Vesicle                                    (49)         (50)         (50)         (49)                                   
      Carcinoma                                                                               1 (2%)                                
   Testes                                             (50)         (50)         (50)         (50)                                   
      Interstitial Cell, Adenoma                                                 1 (2%)       1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (50)         (50)         (50)         (50)                                   
      Hemangiosarcoma                                  1 (2%)       2 (4%)                                                          
      Histiocytic Sarcoma                                                        1 (2%)                                             
   Lymph Node                                         (5)          (3)          (3)          (6)                                    
      Bronchial, Carcinoma, Metastatic, Pancreas                                              1 (17%)                               
      Iliac, Histiocytic Sarcoma                                                 1 (33%)                                            
      Mediastinal, Carcinoma, Metastatic,                                                                                           
          Harderian Gland                              1 (20%)                                                                      
      Mediastinal, Carcinoma, Metastatic, Pancreas                                            1 (17%)                               
      Mediastinal, Histiocytic Sarcoma                                           1 (33%)                                            
      Pancreatic, Histiocytic Sarcoma                                            1 (33%)                                            
      Renal, Histiocytic Sarcoma                                                 1 (33%)                                            
   Lymph Node, Mandibular                             (43)         (46)         (48)         (47)                                   
      Carcinoma, Metastatic, Harderian Gland           1 (2%)                                                                       
   Lymph Node, Mesenteric                             (47)         (50)         (48)         (48)                                   
      Hepatocholangiocarcinoma, Metastatic, Liver                   1 (2%)                                                          
      Histiocytic Sarcoma                                                        2 (4%)                                             
   Spleen                                             (49)         (50)         (49)         (48)                                   
      Hemangioma                                                                              1 (2%)                                
      Hemangiosarcoma                                  2 (4%)       4 (8%)       1 (2%)       1 (2%)                                
      Histiocytic Sarcoma                                                        2 (4%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Skin                                               (50)         (50)         (50)         (50)                                   
      Fibroma                                                       1 (2%)                                                          
      Squamous Cell Papilloma                                                    1 (2%)                                             
      Subcutaneous Tissue, Hemangiosarcoma             1 (2%)       1 (2%)       1 (2%)                                             
      Subcutaneous Tissue, Histiocytic Sarcoma                                   1 (2%)                                             
      Subcutaneous Tissue, Sarcoma                     1 (2%)                                                                       
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  11                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 04/26/05  
Route: GAVAGE                                                                                                     Time: 09:25:25  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          0 MG/KG      2.5          12.5         25                                        
                                                                MG/KG        MG/KG        MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
INTEGUMENTARY SYSTEM - cont                                                                                                         
      Subcutaneous Tissue, Schwannoma Malignant                                  1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (50)         (50)         (50)         (50)                                   
      Hepatocholangiocarcinoma, Metastatic, Liver                   1 (2%)                                                          
      Osteoma                                                                    1 (2%)                                             
      Osteosarcoma                                                                            1 (2%)                                
      Cranium, Carcinoma, Metastatic, Harderian                                                                                     
          Gland                                                                  1 (2%)                                             
      Vertebra, Hemangiosarcoma                                     1 (2%)                                                          
   Skeletal Muscle                                                 (4)          (1)          (1)                                    
      Carcinoma, Metastatic, Intestine Small, Ileum                                           1 (100%)                              
      Hemangiosarcoma, Multiple                                     1 (25%)                                                         
      Hepatocholangiocarcinoma, Metastatic, Liver                   1 (25%)                                                         
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (50)         (50)         (50)         (50)                                   
      Alveolar/Bronchiolar Adenoma                     9 (18%)      5 (10%)      8 (16%)      4 (8%)                                
      Alveolar/Bronchiolar Adenoma, Multiple           2 (4%)       2 (4%)                    2 (4%)                                
      Alveolar/Bronchiolar Carcinoma                   1 (2%)       4 (8%)       4 (8%)       6 (12%)                               
      Alveolar/Bronchiolar Carcinoma, Multiple                                   1 (2%)       1 (2%)                                
      Carcinoma, Metastatic, Harderian Gland                                     1 (2%)                                             
      Carcinoma, Metastatic, Pancreas                                                         1 (2%)                                
      Carcinoma, Metastatic, Intestine Small, Ileum                                           1 (2%)                                
      Hepatoblastoma, Metastatic, Liver                             1 (2%)       1 (2%)                                             
      Hepatocellular Carcinoma, Metastatic, Liver      3 (6%)       4 (8%)       3 (6%)       4 (8%)                                
      Hepatocholangiocarcinoma, Metastatic, Liver                   1 (2%)                                                          
      Histiocytic Sarcoma                                                        1 (2%)                                             
      Sarcoma, Metastatic, Skin                        1 (2%)                                                                       
      Mediastinum, Hepatoblastoma, Metastatic,                                                                                      
           Liver                                                    1 (2%)                                                          
      Mediastinum, Hepatocholangiocarcinoma,                                                                                        
          Metastatic, Liver                                         1 (2%)                                                          
      Mediastinum, Histiocytic Sarcoma                                           1 (2%)                                             
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  12                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 04/26/05  
Route: GAVAGE                                                                                                     Time: 09:25:25  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          0 MG/KG      2.5          12.5         25                                        
                                                                MG/KG        MG/KG        MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
RESPIRATORY SYSTEM - cont                                                                                                           
   Nose                                               (50)         (50)         (50)         (50)                                   
      Carcinoma, Metastatic, Harderian Gland                                     1 (2%)                                             
      Sarcoma, Metastatic, Skin                        1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Eye                                                (45)         (49)         (49)         (47)                                   
      Carcinoma, Metastatic, Harderian Gland           1 (2%)                                                                       
      Sarcoma, Metastatic, Skin                        1 (2%)                                                                       
   Harderian Gland                                    (50)         (50)         (50)         (50)                                   
      Adenoma                                          7 (14%)      1 (2%)      11 (22%)      5 (10%)                               
      Carcinoma                                        2 (4%)                    4 (8%)       1 (2%)                                
      Sarcoma, Metastatic, Skin                        1 (2%)                                                                       
      Bilateral, Adenoma                               1 (2%)                                                                       
      Bilateral, Carcinoma                             1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (49)         (49)         (50)         (48)                                   
      Hepatocholangiocarcinoma, Metastatic, Liver                   1 (2%)                                                          
      Histiocytic Sarcoma                                                        2 (4%)                                             
      Renal Tubule, Adenoma                                         1 (2%)       1 (2%)                                             
      Renal Tubule, Carcinoma                          1 (2%)                                                                       
   Urinary Bladder                                    (48)         (50)         (50)         (48)                                   
      Hemangiosarcoma                                  1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SYSTEMIC LESIONS                                                                                                                   
                                                                                                                                    
   Multiple Organs                                   *(50)        *(50)        *(50)        *(50)                                   
      Histiocytic Sarcoma                                                        3 (6%)                                             
      Lymphoma Malignant                               2 (4%)       2 (4%)       2 (4%)       5 (10%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
     *   Number of animals with any tissue examined microscopically                                                                 
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  13                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 04/26/05  
Route: GAVAGE                                                                                                     Time: 09:25:25  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          0 MG/KG      2.5          12.5         25                                        
                                                                MG/KG        MG/KG        MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
TUMOR SUMMARY                                                                                                                       
                                                                                                                                    
   Total Animals with Primary Neoplasms (b)            46          47          46          47                                       
     Total Primary Neoplasms                           87          90          96          89                                       
                                                                                                                                    
   Total Animals with Benign Neoplasms                 37          38          39          35                                       
     Total Benign Neoplasms                            56          53          61          53                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms              26          23          25          26                                       
     Total Malignant Neoplasms                         31          37          35          36                                       
                                                                                                                                    
   Total Animals with Metastatic Neoplasms              5           6           5           6                                       
     Total Metastatic Neoplasm                         11          16           7          12                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms                                                                                           
   Uncertain Primary Site                                                                                                           
                                                                                                                                    
   Total Animals with Neoplasms Uncertain-                                                                                          
   Benign or Malignant                                                                                                              
     Total Uncertain Neoplasms                                                                                                      
                                                                                                                                    
                                                                                                                                    
____________________________________________________________________________________________________________________________________
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Primary tumors: all tumors except metastatic tumors                                                                              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  14                                                               
                                                                                                                                   
                             ------------------------------------------------------------                                           
                             ----------              END OF REPORT             ----------                                           
                             ------------------------------------------------------------