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TDMS Study 05111-02 Pathology Tables

NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: Mason                                               1,2,3-TRICHLOROPROPANE                                DATE: 09/14/94       
EXPERIMENT: 05111 TEST: 02                                                                                     TIME: 08:56:33       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                   PAGE:       1        
CONT: NO1-ES-45048          ROUTE: GAVAGE                                                                      NTP C#: C60220       
PATHOLOGIST: K. MURTHY                                                                                         CAS: 96-18-4         
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               REASONS FOR REMOVAL:    ALL                                                                                          
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
               REMOVAL DATE RANGE:     ALL                                                                                          
                                                                                                                                    
               TREATMENT GROUPS:       INCLUDE ALL                                                                                  
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08    
LAB: Mason                                               1,2,3-TRICHLOROPROPANE                                DATE: 09/14/94      
EXPERIMENT: 05111 TEST: 02                                                                                     TIME: 08:56:33      
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                       
CONT: NO1-ES-45048          ROUTE: GAVAGE                                                                      NTP C#: C60220      
PATHOLOGIST: K. MURTHY                                                                                         CAS: 96-18-4        
 
 
 
 
 
                                                    MICE(B6C3F1)
                                              ----------------------------
             FOR ALL DOSES THE TUMOR RATES IN THE FOLLOWING TISSUES/ORGANS ARE BASED ON NUMBER OF TISSUES EXAMINED.
             IN OTHER TISSUES/ORGANS, RATES ARE BASED ON NUMBER OF ANIMALS NECROPSIED.
 
 
                                                    ----------------
 
                                                    Adrenal Gland
                                                    Bone Marrow
                                                    Brain
                                                    Clitoral Gland
                                                    Epididymis
                                                    Gallbladder
                                                    Heart
                                                    Islets, Pancreatic
                                                    Kidney
                                                    Liver
                                                    Lung
                                                    Nose
                                                    Ovary
                                                    Pancreas
                                                    Parathyroid Gland
                                                    Pituitary Gland
                                                    Preputial Gland
                                                    Prostate
                                                    Salivary Glands
                                                    Spleen
                                                    Testes
                                                    Thymus
                                                    Thyroid Gland
                                                    Urinary Bladder
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: Mason                                               1,2,3-TRICHLOROPROPANE                                DATE: 09/14/94       
EXPERIMENT: 05111 TEST: 02                                                                                     TIME: 08:56:33       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                        
CONT: NO1-ES-45048          ROUTE: GAVAGE                                                                      NTP C#: C60220       
PATHOLOGIST: K. MURTHY                                                                                         CAS: 96-18-4         
 
 
 
 
 
                                     SUMMARY OF STATISTICALLY SIGNIFICANT (P<=.05) RESULTS
                                     IN THE ANALYSIS OF THE STUDY OF 1,2,3-TRICHLOROPROPANE
 
              ====================================================================================================
 
                                                           MALE MICE
                                                          -------------
                                 ORGAN                               MORPHOLOGY
                                -------                             ------------
 
                             Harderian Gland               Adenoma
                             Liver                         Hemangiosarcoma
                                                           Hepatocellular Adenoma
                                                           Hepatocellular Carcinoma
                                                           Hepatocellular Carcinoma or Hepatocellular Adenoma
                                                           Hepatocellular Carcinoma, Hepatocellular Adenoma, or Hepatoblastoma
                             Lung                          Alveolar/Bronchiolar Adenoma
                                                           Alveolar/Bronchiolar Carcinoma
                                                           Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma
                             Oral Cavity (Oral Mucosa, Tongue, Pharynx, Tooth, Lip)
                                                           Papilloma Squamous or Papilloma
                                                           Squamous Cell Carcinoma, Papilloma Squamous, or Papilloma
                             Stomach, Forestomach          Squamous Cell Carcinoma
                                                           Squamous Cell Carcinoma or Papilloma Squamous
                                                           Squamous Cell Papilloma
                             Tongue                        Squamous Cell Papilloma
                             All Organs                    Hemangiosarcoma
                                                           Hemangiosarcoma or Hemangioma
                                                           Malignant Lymphoma And Histiocytic Sarcoma
                                                           Benign Tumors
                                                           Malignant Tumors
                                                           Malignant and Benign Tumors
 
              ====================================================================================================
 
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: Mason                                               1,2,3-TRICHLOROPROPANE                                DATE: 09/14/94       
EXPERIMENT: 05111 TEST: 02                                                                                     TIME: 08:56:33       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                        
CONT: NO1-ES-45048          ROUTE: GAVAGE                                                                      NTP C#: C60220       
PATHOLOGIST: K. MURTHY                                                                                         CAS: 96-18-4         
 
 
 
 
 
                                     SUMMARY OF STATISTICALLY SIGNIFICANT (P<=.05) RESULTS
                                     IN THE ANALYSIS OF THE STUDY OF 1,2,3-TRICHLOROPROPANE
 
              ====================================================================================================
 
                                                           FEMALE MICE
                                                          -------------
                                 ORGAN                               MORPHOLOGY
                                -------                             ------------
 
                             Harderian Gland               Adenoma
                             Liver                         Hepatocellular Adenoma
                                                           Hepatocellular Carcinoma
                                                           Hepatocellular Carcinoma or Hepatocellular Adenoma
                                                           Hepatocellular Carcinoma, Hepatocellular Adenoma, or Hepatoblastoma
                             Lung                          Alveolar/Bronchiolar Adenoma
                                                           Alveolar/Bronchiolar Carcinoma
                                                           Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma
                             Mammary Gland                 Adenoacanthoma
                             Oral Cavity (Oral Mucosa, Tongue, Pharynx, Tooth, Lip)
                                                           Squamous Cell Carcinoma
                                                           Squamous Cell Carcinoma, Papilloma Squamous, or Papilloma
                             Pharynx                       Squamous Cell Carcinoma
                             Pituitary Gland: Pars Distalis or Unspecified Site
                                                           Adenoma
                             Skin: Subcutaneous Tissue     Sarcoma
                             Stomach, Forestomach          Squamous Cell Carcinoma
                                                           Squamous Cell Carcinoma or Papilloma Squamous
                                                           Squamous Cell Papilloma
                             Uterus                        Adenoma
                                                           Carcinoma
                                                           Polyp Stromal
                             All Organs                    Hemangiosarcoma or Hemangioma
                                                           Histiocytic Sarcoma
                                                           Malignant Lymphoma And Histiocytic Sarcoma
                                                           Malignant Lymphoma: Histiocytic, Lymphocytic, Mixed,
                                                            NOS, or Undifferentiated Cell Type
                                                           Benign Tumors
                                                           Malignant Tumors
                                                           Malignant and Benign Tumors
 
              ====================================================================================================
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 02                                                 PAGE   1
                           STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1,2,3-TRICHLOROPROPANE
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Harderian Gland                                                                                                                  |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  1/60 (2%)    2/59 (3%)   10/60 (17%)  11/60 (18%) |  3/60 (5%)    6/60 (10%)   7/60 (12%)  10/60 (17%) |
|ADJUSTED (b)           |   2.4%         6.5%        44.3%        49.2%      |   6.5%        26.8%        39.0%        57.2%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     0/6 (0%)     0/4 (0%)   |  1/10 (10%)   0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          1/6 (17%)  |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          2/9 (22%)  |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          2/10 (20%)   0/0        |  0/0          0/0          2/9 (22%)    0/0        |
|TERMINAL (d)           |  1/42 (2%)    0/18 (0%)    0/0          0/0        |  2/41 (5%)    2/13 (15%)   0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      639          505          452       |   461 (I)      558          545          445       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.323      P<0.001 **   P<0.001 ** |  P<0.001 **   P=0.036 *    P=0.013 *    P<0.001 ** |
|INCIDENTAL TUMOR       |  P=0.016 *    P=0.765N     P=0.282      P=0.143    |  P=0.031 *    P=0.294      P=0.700N     P=0.642    |
|LOGISTIC REGRESSION    |  P=0.005 **   P=0.449      P=0.002 **   P=0.008 ** |  P=0.002 **   P=0.191      P=0.579N     P=0.250    |
|COCHRAN-ARMITAGE       |  P=0.001 **                                        |  P=0.040 *                                         |
|FISHER EXACT           |               P=0.494      P=0.004 **   P=0.002 ** |               P=0.245      P=0.161      P=0.037 *  |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hemangiosarcoma                                                                                                             |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  3/60 (5%)    0/59 (0%)    0/60 (0%)    0/60 (0%)  |  0/60 (0%)    0/60 (0%)    1/60 (2%)    0/60 (0%)  |
|ADJUSTED (b)           |   6.6%         0.0%         0.0%         0.0%      |   0.0%         0.0%         7.7%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     0/6 (0%)     0/4 (0%)   |  0/10 (0%)    0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          0/10 (0%)    0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  1/42 (2%)    0/18 (0%)    0/0          0/0        |  0/41 (0%)    0/13 (0%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   533          ---          ---          ---       |   ---          ---          603          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.515N     P=0.196N     P=0.433N     P=0.740N   |  P=0.771        (e)        P=0.250        (e)      |
|INCIDENTAL TUMOR       |  P=0.093N     P=0.039N*    P=0.005N**   P=0.096N   |  P=0.950        (e)        P=0.965        (e)      |
|LOGISTIC REGRESSION    |  P=0.175N     P=0.118N     P=0.122N     P=0.162N   |  P=0.851        (e)        P=0.468        (e)      |
|COCHRAN-ARMITAGE       |  P=0.134N                                          |  P=0.786N                                          |
|FISHER EXACT           |               P=0.125N     P=0.122N     P=0.122N   |                 (e)        P=0.500        (e)      |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 02                                                 PAGE   2
                           STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1,2,3-TRICHLOROPROPANE
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Adenoma                                                                                                      |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 12/60 (20%)  18/59 (31%)  21/60 (35%)  31/60 (52%) |  7/60 (12%)   9/60 (15%)   9/60 (15%)  36/60 (60%) |
|ADJUSTED (b)           |  25.1%        61.9%        72.2%       100.0%      |  16.1%        47.7%        65.0%        97.1%      |
|INTERIM SACRIFICE 1 (c)|  1/8 (13%)    0/8 (0%)     0/6 (0%)     2/4 (50%)  |  1/10 (10%)   0/10 (0%)    1/9 (11%)    5/5 (100%) |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          5/6 (83%)  |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          9/9 (100%) |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          4/10 (40%)   0/0        |  0/0          0/0          6/9 (67%)    0/0        |
|TERMINAL (d)           |  7/42 (17%)   9/18 (50%)   0/0          0/0        |  6/41 (15%)   5/13 (38%)   0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   457 (I)      520          410          322       |   461 (I)      540          454          420       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.003 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P=0.011 *    P=0.173      P<0.001 ** |
|INCIDENTAL TUMOR       |  P=0.009 **   P=0.233      P=0.628      P=0.052    |  P<0.001 **   P=0.071      P=0.637      P<0.001 ** |
|LOGISTIC REGRESSION    |  P<0.001 **   P=0.073      P=0.028 *    P=0.007 ** |  P<0.001 **   P=0.164      P=0.057      P<0.001 ** |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P<0.001 **                                        |
|FISHER EXACT           |               P=0.134      P=0.051      P<0.001 ** |               P=0.395      P=0.395      P<0.001 ** |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma                                                                                                    |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  4/60 (7%)   11/59 (19%)   6/60 (10%)   3/60 (5%)  |  1/60 (2%)    3/60 (5%)    0/60 (0%)    2/60 (3%)  |
|ADJUSTED (b)           |   9.2%        40.6%        32.4%        15.6%      |   2.4%        13.2%         0.0%        14.4%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     1/6 (17%)    0/4 (0%)   |  0/10 (0%)    0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          1/9 (11%)  |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          2/10 (20%)   0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  3/42 (7%)    4/18 (22%)   0/0          0/0        |  1/41 (2%)    1/13 (8%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   592          577          457 (I)      389       |   733 (T)      582          ---          494       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.004 **   P=0.002 **   P=0.034 *    P=0.182    |  P<0.001 **   P=0.100        (e)        P=0.036 *  |
|INCIDENTAL TUMOR       |  P=0.456      P=0.137      P=0.718      P=0.721N   |  P=0.418      P=0.430        (e)        P=0.874    |
|LOGISTIC REGRESSION    |  P=0.533      P=0.015 *    P=0.194      P=0.666    |  P=0.259      P=0.242        (e)        P=0.395    |
|COCHRAN-ARMITAGE       |  P=0.113N                                          |  P=0.577                                           |
|FISHER EXACT           |               P=0.044 *    P=0.372      P=0.500N   |               P=0.309      P=0.500N     P=0.500    |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 02                                                 PAGE   3
                           STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1,2,3-TRICHLOROPROPANE
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma or Hepatocellular Adenoma                                                                          |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 14/60 (23%)  24/59 (41%)  25/60 (42%)  33/60 (55%) |  8/60 (13%)  11/60 (18%)   9/60 (15%)  36/60 (60%) |
|ADJUSTED (b)           |  29.3%        72.8%        82.9%       100.0%      |  18.5%        55.8%        65.0%        97.1%      |
|INTERIM SACRIFICE 1 (c)|  1/8 (13%)    0/8 (0%)     1/6 (17%)    2/4 (50%)  |  1/10 (10%)   0/10 (0%)    1/9 (11%)    5/5 (100%) |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          5/6 (83%)  |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          9/9 (100%) |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          6/10 (60%)   0/0        |  0/0          0/0          6/9 (67%)    0/0        |
|TERMINAL (d)           |  9/42 (21%)  10/18 (56%)   0/0          0/0        |  7/41 (17%)   6/13 (46%)   0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   457 (I)      520          410          322       |   461 (I)      540          454          420       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P=0.003 **   P=0.173      P<0.001 ** |
|INCIDENTAL TUMOR       |  P=0.002 **   P=0.130      P=0.425      P=0.040 *  |  P<0.001 **   P=0.041 *    P=0.637      P<0.001 ** |
|LOGISTIC REGRESSION    |  P<0.001 **   P=0.079      P=0.007 **   P=0.003 ** |  P<0.001 **   P=0.093      P=0.067      P<0.001 ** |
|COCHRAN-ARMITAGE       |  P=0.001 **                                        |  P<0.001 **                                        |
|FISHER EXACT           |               P=0.033 *    P=0.025 *    P<0.001 ** |               P=0.309      P=0.500      P<0.001 ** |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma, Hepatocellular Adenoma, or Hepatoblastoma                                                         |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 14/60 (23%)  24/59 (41%)  25/60 (42%)  33/60 (55%) |  8/60 (13%)  11/60 (18%)   9/60 (15%)  36/60 (60%) |
|ADJUSTED (b)           |  29.3%        72.8%        82.9%       100.0%      |  18.5%        55.8%        65.0%        97.1%      |
|INTERIM SACRIFICE 1 (c)|  1/8 (13%)    0/8 (0%)     1/6 (17%)    2/4 (50%)  |  1/10 (10%)   0/10 (0%)    1/9 (11%)    5/5 (100%) |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          5/6 (83%)  |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          9/9 (100%) |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          6/10 (60%)   0/0        |  0/0          0/0          6/9 (67%)    0/0        |
|TERMINAL (d)           |  9/42 (21%)  10/18 (56%)   0/0          0/0        |  7/41 (17%)   6/13 (46%)   0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   457 (I)      520          410          322       |   461 (I)      540          454          420       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P=0.003 **   P=0.173      P<0.001 ** |
|INCIDENTAL TUMOR       |  P=0.002 **   P=0.130      P=0.425      P=0.040 *  |  P<0.001 **   P=0.041 *    P=0.637      P<0.001 ** |
|LOGISTIC REGRESSION    |  P<0.001 **   P=0.079      P=0.007 **   P=0.003 ** |  P<0.001 **   P=0.093      P=0.067      P<0.001 ** |
|COCHRAN-ARMITAGE       |  P=0.001 **                                        |  P<0.001 **                                        |
|FISHER EXACT           |               P=0.033 *    P=0.025 *    P<0.001 ** |               P=0.309      P=0.500      P<0.001 ** |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 02                                                 PAGE   4
                           STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1,2,3-TRICHLOROPROPANE
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Adenoma                                                                                                |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  7/60 (12%)  12/59 (20%)   3/60 (5%)    9/60 (15%) |  4/60 (7%)    3/60 (5%)    0/60 (0%)   11/60 (18%) |
|ADJUSTED (b)           |  15.9%        37.7%        10.4%        34.0%      |   9.4%        17.5%         0.0%        43.0%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     1/8 (13%)    0/6 (0%)     3/4 (75%)  |  0/10 (0%)    0/10 (0%)    0/9 (0%)     1/5 (20%)  |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          1/9 (11%)  |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          0/10 (0%)    0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  6/42 (14%)   4/18 (22%)   0/0          0/0        |  3/41 (7%)    2/13 (15%)   0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   458          424          388          435       |   699          574          ---          379       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.013 *    P=0.196      P<0.001 ** |  P<0.001 **   P=0.314        (e)        P<0.001 ** |
|INCIDENTAL TUMOR       |  P=0.225      P=0.158      P=0.543N     P=0.137    |  P=0.002 **   P=0.651        (e)        P=0.177    |
|LOGISTIC REGRESSION    |  P=0.354      P=0.127      P=0.315N     P=0.280    |  P<0.001 **   P=0.585      P=0.939N     P=0.054    |
|COCHRAN-ARMITAGE       |  P=0.555                                           |  P=0.002 **                                        |
|FISHER EXACT           |               P=0.149      P=0.161N     P=0.395    |               P=0.500N     P=0.059N     P=0.048 *  |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Carcinoma                                                                                              |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  1/60 (2%)    0/59 (0%)    2/60 (3%)    0/60 (0%)  |  3/60 (5%)    0/60 (0%)    0/60 (0%)    0/60 (0%)  |
|ADJUSTED (b)           |   2.4%         0.0%        14.1%         0.0%      |   6.9%         0.0%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     0/6 (0%)     0/4 (0%)   |  0/10 (0%)    0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          0/10 (0%)    0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  1/42 (2%)    0/18 (0%)    0/0          0/0        |  2/41 (5%)    0/13 (0%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      ---          590          ---       |   631          ---          ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.221      P=0.669N     P=0.045 *      (e)      |  P=0.999N     P=0.324N       (e)          (e)      |
|INCIDENTAL TUMOR       |  P=0.756      P=0.669N     P=0.754        (e)      |  P=0.747N     P=0.072N     P=0.035N*      (e)      |
|LOGISTIC REGRESSION    |  P=0.761      P=0.669N     P=0.234      P=0.280    |  P=0.645N     P=0.181N     P=0.502N     P=0.794N   |
|COCHRAN-ARMITAGE       |  P=0.454N                                          |  P=0.135N                                          |
|FISHER EXACT           |               P=0.504N     P=0.500      P=0.500N   |               P=0.122N     P=0.122N     P=0.122N   |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 02                                                 PAGE   5
                           STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1,2,3-TRICHLOROPROPANE
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma                                                              |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  8/60 (13%)  12/59 (20%)   5/60 (8%)    9/60 (15%) |  7/60 (12%)   3/60 (5%)    0/60 (0%)   11/60 (18%) |
|ADJUSTED (b)           |  18.2%        37.7%        23.1%        34.0%      |  16.1%        17.5%         0.0%        43.0%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     1/8 (13%)    0/6 (0%)     3/4 (75%)  |  0/10 (0%)    0/10 (0%)    0/9 (0%)     1/5 (20%)  |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          1/9 (11%)  |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          0/10 (0%)    0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  7/42 (17%)   4/18 (22%)   0/0          0/0        |  5/41 (12%)   2/13 (15%)   0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   458          424          388          435       |   631          574          ---          379       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.021 *    P=0.020 *    P<0.001 ** |  P<0.001 **   P=0.588        (e)        P<0.001 ** |
|INCIDENTAL TUMOR       |  P=0.196      P=0.207      P=0.641N     P=0.137    |  P=0.004 **   P=0.241N     P=0.035N*    P=0.177    |
|LOGISTIC REGRESSION    |  P=0.347      P=0.182      P=0.616N     P=0.300    |  P<0.001 **   P=0.363N     P=0.305N     P=0.103    |
|COCHRAN-ARMITAGE       |  P=0.496N                                          |  P=0.022 *                                         |
|FISHER EXACT           |               P=0.219      P=0.279N     P=0.500    |               P=0.161N     P=0.006N**   P=0.222    |
|=================================================================================================================================|
|                                                                                                                                 |
|Mammary Gland                                                                                                                    |
|     Adenoacanthoma                                                                                                              |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    0/59 (0%)    0/60 (0%)    0/60 (0%)  |  0/60 (0%)    0/60 (0%)    1/60 (2%)    3/60 (5%)  |
|ADJUSTED (b)           |   0.0%         0.0%         0.0%         0.0%      |   0.0%         0.0%         2.7%        20.0%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     0/6 (0%)     0/4 (0%)   |  0/10 (0%)    0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          1/6 (17%)  |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          0/10 (0%)    0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  0/42 (0%)    0/18 (0%)    0/0          0/0        |  0/41 (0%)    0/13 (0%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   ---          ---          ---          ---       |   ---          ---          494          341       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |    (e)          (e)          (e)          (e)      |  P=0.034 *      (e)        P=0.452      P=0.203    |
|INCIDENTAL TUMOR       |    (e)          (e)          (e)          (e)      |  P=0.390        (e)        P=0.898      P=0.580    |
|LOGISTIC REGRESSION    |    (e)          (e)          (e)          (e)      |  P=0.073        (e)        P=0.594      P=0.283    |
|COCHRAN-ARMITAGE       |    (e)                                             |  P=0.021 *                                         |
|FISHER EXACT           |                 (e)          (e)          (e)      |                 (e)        P=0.500      P=0.122    |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 02                                                 PAGE   6
                           STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1,2,3-TRICHLOROPROPANE
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Mammary Gland                                                                                                                    |
|     Carcinoma                                                                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    0/59 (0%)    0/60 (0%)    0/60 (0%)  |  0/60 (0%)    2/60 (3%)    0/60 (0%)    1/60 (2%)  |
|ADJUSTED (b)           |   0.0%         0.0%         0.0%         0.0%      |   0.0%         6.6%         0.0%         2.9%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     0/6 (0%)     0/4 (0%)   |  0/10 (0%)    0/10 (0%)    0/9 (0%)     1/5 (20%)  |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          0/10 (0%)    0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  0/42 (0%)    0/18 (0%)    0/0          0/0        |  0/41 (0%)    0/13 (0%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   ---          ---          ---          ---       |   ---          583          ---          461 (I)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |    (e)          (e)          (e)          (e)      |  P=0.087      P=0.156        (e)        P=0.362    |
|INCIDENTAL TUMOR       |    (e)          (e)          (e)          (e)      |  P=0.176      P=0.660        (e)        P=0.362    |
|LOGISTIC REGRESSION    |    (e)          (e)          (e)          (e)      |  P=0.622      P=0.242        (e)        P=0.643    |
|COCHRAN-ARMITAGE       |    (e)                                             |  P=0.622                                           |
|FISHER EXACT           |                 (e)          (e)          (e)      |               P=0.248        (e)        P=0.500    |
|=================================================================================================================================|
|                                                                                                                                 |
|Oral Cavity (Oral Mucosa, Tongue, Pharynx, Tooth, Lip)                                                                           |
|     Papilloma Squamous or Papilloma                                                                                             |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    0/59 (0%)    0/60 (0%)    2/60 (3%)  |  1/60 (2%)    0/60 (0%)    1/60 (2%)    0/60 (0%)  |
|ADJUSTED (b)           |   0.0%         0.0%         0.0%        14.2%      |   2.4%         0.0%         5.9%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     0/6 (0%)     0/4 (0%)   |  0/10 (0%)    0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          1/9 (11%)  |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          0/10 (0%)    0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  0/42 (0%)    0/18 (0%)    0/0          0/0        |  1/41 (2%)    0/13 (0%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   ---          ---          ---          470       |   733 (T)      ---          578          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.137        (e)          (e)        P=0.395    |  P=0.829      P=0.728N     P=0.302        (e)      |
|INCIDENTAL TUMOR       |  P=0.467        (e)          (e)        P=0.906    |  P=0.959      P=0.728N     P=0.965        (e)      |
|LOGISTIC REGRESSION    |  P=0.075        (e)          (e)        P=0.311    |  P=0.881N     P=0.728N     P=0.629        (e)      |
|COCHRAN-ARMITAGE       |  P=0.042 *                                         |  P=0.464N                                          |
|FISHER EXACT           |                 (e)          (e)        P=0.248    |               P=0.500N     P=0.752N     P=0.500N   |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 02                                                 PAGE   7
                           STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1,2,3-TRICHLOROPROPANE
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Oral Cavity (Oral Mucosa, Tongue, Pharynx, Tooth, Lip)                                                                           |
|     Squamous Cell Carcinoma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    0/59 (0%)    0/60 (0%)    0/60 (0%)  |  0/60 (0%)    0/60 (0%)    1/60 (2%)    5/60 (8%)  |
|ADJUSTED (b)           |   0.0%         0.0%         0.0%         0.0%      |   0.0%         0.0%         4.2%        16.3%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     0/6 (0%)     0/4 (0%)   |  0/10 (0%)    0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          0/10 (0%)    0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  0/42 (0%)    0/18 (0%)    0/0          0/0        |  0/41 (0%)    0/13 (0%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   ---          ---          ---          ---       |   ---          ---          551          427       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |    (e)          (e)          (e)          (e)      |  P<0.001 **     (e)        P=0.370      P=0.006 ** |
|INCIDENTAL TUMOR       |    (e)          (e)          (e)          (e)      |  P=0.056        (e)        P=0.965      P=0.653    |
|LOGISTIC REGRESSION    |    (e)          (e)          (e)          (e)      |  P=0.008 **     (e)        P=0.552      P=0.128    |
|COCHRAN-ARMITAGE       |    (e)                                             |  P=0.001 **                                        |
|FISHER EXACT           |                 (e)          (e)          (e)      |                 (e)        P=0.500      P=0.029 *  |
|=================================================================================================================================|
|                                                                                                                                 |
|Oral Cavity (Oral Mucosa, Tongue, Pharynx, Tooth, Lip)                                                                           |
|     Squamous Cell Carcinoma, Papilloma Squamous, or Papilloma                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    0/59 (0%)    0/60 (0%)    2/60 (3%)  |  1/60 (2%)    0/60 (0%)    2/60 (3%)    5/60 (8%)  |
|ADJUSTED (b)           |   0.0%         0.0%         0.0%        14.2%      |   2.4%         0.0%         9.8%        16.3%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     0/6 (0%)     0/4 (0%)   |  0/10 (0%)    0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          1/9 (11%)  |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          0/10 (0%)    0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  0/42 (0%)    0/18 (0%)    0/0          0/0        |  1/41 (2%)    0/13 (0%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   ---          ---          ---          470       |   733 (T)      ---          551          427       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.137        (e)          (e)        P=0.395    |  P<0.001 **   P=0.728N     P=0.086      P=0.006 ** |
|INCIDENTAL TUMOR       |  P=0.467        (e)          (e)        P=0.906    |  P=0.044 *    P=0.728N     P=0.872      P=0.653    |
|LOGISTIC REGRESSION    |  P=0.075        (e)          (e)        P=0.311    |  P=0.024 *    P=0.728N     P=0.365      P=0.212    |
|COCHRAN-ARMITAGE       |  P=0.042 *                                         |  P=0.011 *                                         |
|FISHER EXACT           |                 (e)          (e)        P=0.248    |               P=0.500N     P=0.500      P=0.103    |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 02                                                 PAGE   8
                           STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1,2,3-TRICHLOROPROPANE
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Pharynx                                                                                                                          |
|     Squamous Cell Carcinoma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    0/59 (0%)    0/60 (0%)    0/60 (0%)  |  0/60 (0%)    0/60 (0%)    1/60 (2%)    5/60 (8%)  |
|ADJUSTED (b)           |   0.0%         0.0%         0.0%         0.0%      |   0.0%         0.0%         4.2%        16.3%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     0/6 (0%)     0/4 (0%)   |  0/10 (0%)    0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          0/10 (0%)    0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  0/42 (0%)    0/18 (0%)    0/0          0/0        |  0/41 (0%)    0/13 (0%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   ---          ---          ---          ---       |   ---          ---          551          427       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |    (e)          (e)          (e)          (e)      |  P<0.001 **     (e)        P=0.370      P=0.006 ** |
|INCIDENTAL TUMOR       |    (e)          (e)          (e)          (e)      |  P=0.056        (e)        P=0.965      P=0.653    |
|LOGISTIC REGRESSION    |    (e)          (e)          (e)          (e)      |  P=0.008 **     (e)        P=0.552      P=0.128    |
|COCHRAN-ARMITAGE       |    (e)                                             |  P=0.001 **                                        |
|FISHER EXACT           |                 (e)          (e)          (e)      |                 (e)        P=0.500      P=0.029 *  |
|=================================================================================================================================|
|                                                                                                                                 |
|Pituitary Gland: Pars Distalis or Unspecified Site                                                                               |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/54 (0%)    0/56 (0%)    0/56 (0%)    0/58 (0%)  |  4/58 (7%)    2/56 (4%)    0/54 (0%)    0/58 (0%)  |
|ADJUSTED (b)           |   0.0%         0.0%         0.0%         0.0%      |   9.1%        14.1%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     0/6 (0%)     0/4 (0%)   |  1/10 (10%)   0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          0/10 (0%)    0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  0/37 (0%)    0/18 (0%)    0/0          0/0        |  3/41 (7%)    1/12 (8%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   ---          ---          ---          ---       |   463 (I)      719          ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |    (e)          (e)          (e)          (e)      |  P=0.669N     P=0.568      P=0.521N     P=0.638N   |
|INCIDENTAL TUMOR       |    (e)          (e)          (e)          (e)      |  P=0.618N     P=0.629N     P=0.521N     P=0.638N   |
|LOGISTIC REGRESSION    |    (e)          (e)          (e)          (e)      |  P=0.339N     P=0.480N     P=0.135N     P=0.218N   |
|COCHRAN-ARMITAGE       |    (e)                                             |  P=0.043N*                                         |
|FISHER EXACT           |                 (e)          (e)          (e)      |               P=0.356N     P=0.068N     P=0.059N   |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 02                                                 PAGE   9
                           STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1,2,3-TRICHLOROPROPANE
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin: Subcutaneous Tissue                                                                                                        |
|     Sarcoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    1/59 (2%)    0/60 (0%)    0/60 (0%)  |  2/60 (3%)    0/60 (0%)    0/60 (0%)    0/60 (0%)  |
|ADJUSTED (b)           |   0.0%         5.6%         0.0%         0.0%      |   4.6%         0.0%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     0/6 (0%)     0/4 (0%)   |  0/10 (0%)    0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          0/10 (0%)    0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  0/42 (0%)    1/18 (6%)    0/0          0/0        |  0/41 (0%)    0/13 (0%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   ---          729 (T)      ---          ---       |   699          ---          ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=1.000      P=0.331        (e)          (e)      |  P=1.000N     P=0.482N       (e)          (e)      |
|INCIDENTAL TUMOR       |  P=1.000      P=0.331        (e)          (e)      |  P=0.999N     P=0.045N*      (e)          (e)      |
|LOGISTIC REGRESSION    |  P=1.000      P=0.331        (e)          (e)      |  P=0.966N     P=0.354N     P=0.900N     P=0.994N   |
|COCHRAN-ARMITAGE       |  P=0.575N                                          |  P=0.243N                                          |
|FISHER EXACT           |               P=0.496        (e)          (e)      |               P=0.248N     P=0.248N     P=0.248N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Spleen                                                                                                                           |
|     Hemangiosarcoma                                                                                                             |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  2/60 (3%)    0/59 (0%)    0/60 (0%)    0/60 (0%)  |  0/59 (0%)    0/60 (0%)    0/60 (0%)    0/60 (0%)  |
|ADJUSTED (b)           |   4.6%         0.0%         0.0%         0.0%      |   0.0%         0.0%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     0/6 (0%)     0/4 (0%)   |  0/10 (0%)    0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          0/10 (0%)    0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  1/42 (2%)    0/18 (0%)    0/0          0/0        |  0/41 (0%)    0/13 (0%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   615          ---          ---          ---       |   ---          ---          ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.971N     P=0.366N     P=0.761N       (e)      |    (e)          (e)          (e)          (e)      |
|INCIDENTAL TUMOR       |  P=0.630N     P=0.121N     P=0.099N     P=0.638N   |    (e)          (e)          (e)          (e)      |
|LOGISTIC REGRESSION    |  P=0.552N     P=0.265N     P=0.459N     P=0.660N   |    (e)          (e)          (e)          (e)      |
|COCHRAN-ARMITAGE       |  P=0.242N                                          |    (e)                                             |
|FISHER EXACT           |               P=0.252N     P=0.248N     P=0.248N   |                 (e)          (e)          (e)      |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 02                                                 PAGE  10
                           STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1,2,3-TRICHLOROPROPANE
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Stomach, Forestomach                                                                                                             |
|     Squamous Cell Carcinoma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)   41/59 (69%)  54/60 (90%)  55/60 (92%) |  0/60 (0%)   47/60 (78%)  55/60 (92%)  51/60 (85%) |
|ADJUSTED (b)           |   0.0%        86.6%       100.0%        96.5%      |   0.0%        95.9%       100.0%        97.9%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     1/8 (13%)    4/6 (67%)    4/4 (100%) |  0/10 (0%)    1/10 (10%)   6/9 (67%)    2/5 (40%)  |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          5/6 (83%)  |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          7/9 (78%)  |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0         10/10 (100%)  0/0        |  0/0          0/0          9/9 (100%)   0/0        |
|TERMINAL (d)           |  0/42 (0%)   12/18 (67%)   0/0          0/0        |  0/41 (0%)   11/13 (85%)   0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   ---          424          385          350       |   ---          414          312          295       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|LOGISTIC REGRESSION    |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P<0.001 **                                        |
|FISHER EXACT           |               P<0.001 **   P<0.001 **   P<0.001 ** |               P<0.001 **   P<0.001 **   P<0.001 ** |
|=================================================================================================================================|
|                                                                                                                                 |
|Stomach, Forestomach                                                                                                             |
|     Squamous Cell Carcinoma or Papilloma Squamous                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  3/60 (5%)   57/59 (97%)  57/60 (95%)  59/60 (98%) |  0/60 (0%)   54/60 (90%)  59/60 (98%)  59/60 (98%) |
|ADJUSTED (b)           |   6.7%       100.0%       100.0%       100.0%      |   0.0%       100.0%       100.0%       100.0%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     7/8 (88%)    4/6 (67%)    4/4 (100%) |  0/10 (0%)    6/10 (60%)   9/9 (100%)   5/5 (100%) |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          6/6 (100%) |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          9/9 (100%) |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0         10/10 (100%)  0/0        |  0/0          0/0          9/9 (100%)   0/0        |
|TERMINAL (d)           |  2/42 (5%)   18/18 (100%)  0/0          0/0        |  0/41 (0%)   13/13 (100%)  0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   486          424          385          322       |   ---          414          312          295       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|LOGISTIC REGRESSION    |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P<0.001 **                                        |
|FISHER EXACT           |               P<0.001 **   P<0.001 **   P<0.001 ** |               P<0.001 **   P<0.001 **   P<0.001 ** |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 02                                                 PAGE  11
                           STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1,2,3-TRICHLOROPROPANE
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Stomach, Forestomach                                                                                                             |
|     Squamous Cell Papilloma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  3/60 (5%)   35/59 (59%)  25/60 (42%)  35/60 (58%) |  0/60 (0%)   28/60 (47%)  27/60 (45%)  33/60 (55%) |
|ADJUSTED (b)           |   6.7%        88.0%        83.7%        90.0%      |   0.0%        84.8%        73.1%        94.1%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     7/8 (88%)    3/6 (50%)    2/4 (50%)  |  0/10 (0%)    5/10 (50%)   9/9 (100%)   4/5 (80%)  |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          5/6 (83%)  |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          6/9 (67%)  |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          8/10 (80%)   0/0        |  0/0          0/0          5/9 (56%)    0/0        |
|TERMINAL (d)           |  2/42 (5%)   14/18 (78%)   0/0          0/0        |  0/41 (0%)    9/13 (69%)   0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   486          457 (I)      445          322       |   ---          461 (I)      442          377       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P=0.004 **   P<0.001 **   P=0.045 *    P=0.014 *  |  P<0.001 **   P<0.001 **   P<0.001 **   P=0.001 ** |
|LOGISTIC REGRESSION    |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P<0.001 **                                        |
|FISHER EXACT           |               P<0.001 **   P<0.001 **   P<0.001 ** |               P<0.001 **   P<0.001 **   P<0.001 ** |
|=================================================================================================================================|
|                                                                                                                                 |
|Thyroid Gland: Follicular Cell                                                                                                   |
|     Carcinoma or Adenoma                                                                                                        |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  1/58 (2%)    1/59 (2%)    2/60 (3%)    0/60 (0%)  |  0/59 (0%)    0/59 (0%)    0/58 (0%)    0/59 (0%)  |
|ADJUSTED (b)           |   2.4%         5.6%         3.7%         0.0%      |   0.0%         0.0%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     1/6 (17%)    0/4 (0%)   |  0/10 (0%)    0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          0/10 (0%)    0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  1/41 (2%)    1/18 (6%)    0/0          0/0        |  0/41 (0%)    0/13 (0%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      729 (T)      452          ---       |   ---          ---          ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.649      P=0.568      P=0.199        (e)      |    (e)          (e)          (e)          (e)      |
|INCIDENTAL TUMOR       |  P=0.609N     P=0.568      P=0.355        (e)      |    (e)          (e)          (e)          (e)      |
|LOGISTIC REGRESSION    |  P=0.391N     P=0.568      P=0.600        (e)      |    (e)          (e)          (e)          (e)      |
|COCHRAN-ARMITAGE       |  P=0.326N                                          |    (e)                                             |
|FISHER EXACT           |               P=0.748N     P=0.513      P=0.492N   |                 (e)          (e)          (e)      |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 02                                                 PAGE  12
                           STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1,2,3-TRICHLOROPROPANE
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Tongue                                                                                                                           |
|     Squamous Cell Papilloma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    0/59 (0%)    0/60 (0%)    2/60 (3%)  |  0/60 (0%)    0/60 (0%)    1/60 (2%)    0/60 (0%)  |
|ADJUSTED (b)           |   0.0%         0.0%         0.0%        14.2%      |   0.0%         0.0%         5.9%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     0/6 (0%)     0/4 (0%)   |  0/10 (0%)    0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          1/9 (11%)  |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          0/10 (0%)    0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  0/42 (0%)    0/18 (0%)    0/0          0/0        |  0/41 (0%)    0/13 (0%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   ---          ---          ---          470       |   ---          ---          578          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.137        (e)          (e)        P=0.395    |  P=0.789        (e)        P=0.302        (e)      |
|INCIDENTAL TUMOR       |  P=0.467        (e)          (e)        P=0.906    |  P=0.950        (e)        P=0.965        (e)      |
|LOGISTIC REGRESSION    |  P=0.075        (e)          (e)        P=0.311    |  P=0.861        (e)        P=0.517        (e)      |
|COCHRAN-ARMITAGE       |  P=0.042 *                                         |  P=0.786N                                          |
|FISHER EXACT           |                 (e)          (e)        P=0.248    |                 (e)        P=0.500        (e)      |
|=================================================================================================================================|
|                                                                                                                                 |
|Uterus                                                                                                                           |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |                                                    |  0/60 (0%)    1/60 (2%)    0/60 (0%)    4/60 (7%)  |
|ADJUSTED (b)           |                                                    |   0.0%         7.7%         0.0%        25.0%      |
|INTERIM SACRIFICE 1 (c)|                                                    |  0/10 (0%)    0/10 (0%)    0/9 (0%)     1/5 (20%)  |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|                                                    |                                                    |
|INTERIM SACRIFICE 4 (c)|                                                    |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |                                                    |  0/41 (0%)    1/13 (8%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |                                                    |   ---          733 (T)      ---          461 (I)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |                                                    |  P<0.001 **   P=0.272        (e)        P=0.001 ** |
|INCIDENTAL TUMOR       |                                                    |  P=0.027 *    P=0.272        (e)        P=0.288    |
|LOGISTIC REGRESSION    |                                                    |  P=0.009 **   P=0.272        (e)        P=0.134    |
|COCHRAN-ARMITAGE       |                                                    |  P=0.011 *                                         |
|FISHER EXACT           |                                                    |               P=0.500        (e)        P=0.059    |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 02                                                 PAGE  13
                           STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1,2,3-TRICHLOROPROPANE
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Uterus                                                                                                                           |
|     Carcinoma                                                                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |                                                    |  0/60 (0%)    4/60 (7%)    3/60 (5%)    8/60 (13%) |
|ADJUSTED (b)           |                                                    |   0.0%        25.4%        25.3%        64.2%      |
|INTERIM SACRIFICE 1 (c)|                                                    |  0/10 (0%)    0/10 (0%)    0/9 (0%)     2/5 (40%)  |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          3/6 (50%)  |
|INTERIM SACRIFICE 3 (c)|                                                    |                                                    |
|INTERIM SACRIFICE 4 (c)|                                                    |  0/0          0/0          2/9 (22%)    0/0        |
|TERMINAL (d)           |                                                    |  0/41 (0%)    2/13 (15%)   0/0          0/0        |
|FIRST INCIDENCE (DAYS) |                                                    |   ---          698          582          461 (I)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |                                                    |  P<0.001 **   P=0.002 **   P=0.278      P<0.001 ** |
|INCIDENTAL TUMOR       |                                                    |  P=0.001 **   P=0.036 *    P=0.965      P=0.091    |
|LOGISTIC REGRESSION    |                                                    |  P<0.001 **   P=0.007 **   P=0.050 *    P=0.017 *  |
|COCHRAN-ARMITAGE       |                                                    |  P=0.006 **                                        |
|FISHER EXACT           |                                                    |               P=0.059      P=0.122      P=0.003 ** |
|=================================================================================================================================|
|                                                                                                                                 |
|Uterus                                                                                                                           |
|     Polyp Stromal                                                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |                                                    |  0/60 (0%)    2/60 (3%)    2/60 (3%)    7/60 (12%) |
|ADJUSTED (b)           |                                                    |   0.0%        11.2%         3.8%        28.6%      |
|INTERIM SACRIFICE 1 (c)|                                                    |  0/10 (0%)    0/10 (0%)    1/9 (11%)    1/5 (20%)  |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          1/6 (17%)  |
|INTERIM SACRIFICE 3 (c)|                                                    |                                                    |
|INTERIM SACRIFICE 4 (c)|                                                    |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |                                                    |  0/41 (0%)    1/13 (8%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |                                                    |   ---          643          312          379       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |                                                    |  P<0.001 **   P=0.083      P=0.228      P=0.006 ** |
|INCIDENTAL TUMOR       |                                                    |  P=0.033 *    P=0.260      P=0.300      P=0.251    |
|LOGISTIC REGRESSION    |                                                    |  P=0.023 *    P=0.165      P=0.378      P=0.074    |
|COCHRAN-ARMITAGE       |                                                    |  P=0.002 **                                        |
|FISHER EXACT           |                                                    |               P=0.248      P=0.248      P=0.006 ** |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 02                                                 PAGE  14
                           STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1,2,3-TRICHLOROPROPANE
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Hemangioma                                                                                                                  |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  1/60 (2%)    2/59 (3%)    1/60 (2%)    0/60 (0%)  |  1/60 (2%)    1/60 (2%)    1/60 (2%)    0/60 (0%)  |
|ADJUSTED (b)           |   2.4%         6.0%         9.1%         0.0%      |   2.4%         7.7%         8.3%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     0/6 (0%)     0/4 (0%)   |  0/10 (0%)    0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          1/10 (10%)   0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  1/42 (2%)    0/18 (0%)    0/0          0/0        |  1/41 (2%)    1/13 (8%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      541          623 (I)      ---       |   733 (T)      733 (T)      608          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.820N     P=0.344        (e)          (e)      |  P=0.589      P=0.488      P=0.235        (e)      |
|INCIDENTAL TUMOR       |  P=0.292N     P=0.649        (e)          (e)      |  P=0.862      P=0.488      P=0.965        (e)      |
|LOGISTIC REGRESSION    |  P=0.516N     P=0.481      P=0.513      P=0.153N   |  P=0.813      P=0.488      P=0.546        (e)      |
|COCHRAN-ARMITAGE       |  P=0.230N                                          |  P=0.323N                                          |
|FISHER EXACT           |               P=0.494      P=0.752N     P=0.500N   |               P=0.752N     P=0.752N     P=0.500N   |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Hemangiosarcoma                                                                                                             |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  4/60 (7%)    0/59 (0%)    0/60 (0%)    0/60 (0%)  |  0/60 (0%)    0/60 (0%)    1/60 (2%)    0/60 (0%)  |
|ADJUSTED (b)           |   8.9%         0.0%         0.0%         0.0%      |   0.0%         0.0%         7.7%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     0/6 (0%)     0/4 (0%)   |  0/10 (0%)    0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          0/10 (0%)    0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  2/42 (5%)    0/18 (0%)    0/0          0/0        |  0/41 (0%)    0/13 (0%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   533          ---          ---          ---       |   ---          ---          603          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.478N     P=0.142N     P=0.433N     P=0.740N   |  P=0.771        (e)        P=0.250        (e)      |
|INCIDENTAL TUMOR       |  P=0.083N     P=0.032N*    P=0.005N**   P=0.096N   |  P=0.950        (e)        P=0.965        (e)      |
|LOGISTIC REGRESSION    |  P=0.153N     P=0.068N     P=0.096N     P=0.153N   |  P=0.851        (e)        P=0.468        (e)      |
|COCHRAN-ARMITAGE       |  P=0.077N                                          |  P=0.786N                                          |
|FISHER EXACT           |               P=0.061N     P=0.059N     P=0.059N   |                 (e)        P=0.500        (e)      |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 02                                                 PAGE  15
                           STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1,2,3-TRICHLOROPROPANE
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Hemangiosarcoma or Hemangioma                                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  4/60 (7%)    2/59 (3%)    1/60 (2%)    0/60 (0%)  |  1/60 (2%)    1/60 (2%)    2/60 (3%)    0/60 (0%)  |
|ADJUSTED (b)           |   8.9%         6.0%         9.1%         0.0%      |   2.4%         7.7%        15.4%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     0/6 (0%)     0/4 (0%)   |  0/10 (0%)    0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          1/10 (10%)   0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  2/42 (5%)    0/18 (0%)    0/0          0/0        |  1/41 (2%)    1/13 (8%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   533          541          623 (I)      ---       |   733 (T)      733 (T)      603          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.450N     P=0.539N     P=0.433N     P=0.740N   |  P=0.092      P=0.488      P=0.032 *      (e)      |
|INCIDENTAL TUMOR       |  P=0.027N*    P=0.146N     P=0.005N**   P=0.096N   |  P=0.520      P=0.488      P=0.872        (e)      |
|LOGISTIC REGRESSION    |  P=0.078N     P=0.074N     P=0.018N*    P=0.153N   |  P=0.666      P=0.488      P=0.213        (e)      |
|COCHRAN-ARMITAGE       |  P=0.052N                                          |  P=0.333N                                          |
|FISHER EXACT           |               P=0.348N     P=0.182N     P=0.059N   |               P=0.752N     P=0.500      P=0.500N   |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Histiocytic Sarcoma                                                                                                         |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  1/60 (2%)    1/59 (2%)    1/60 (2%)    0/60 (0%)  |  2/60 (3%)    1/60 (2%)    0/60 (0%)    0/60 (0%)  |
|ADJUSTED (b)           |   2.1%         2.9%         3.3%         0.0%      |   4.4%         2.9%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     0/6 (0%)     0/4 (0%)   |  0/10 (0%)    0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          0/10 (0%)    0/0        |  0/0          0/0          0/9 (0%)     0/0        |
|TERMINAL (d)           |  0/42 (0%)    0/18 (0%)    0/0          0/0        |  1/41 (2%)    0/13 (0%)    0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   495          620          540          ---       |   480          600          ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.705N     P=0.730      P=0.685      P=0.665N   |  P=0.589N     P=0.661N     P=0.556N     P=0.669N   |
|INCIDENTAL TUMOR       |  P=0.099N     P=0.494N     P=0.355N     P=0.094N   |  P=0.101N     P=0.352N     P=0.102N     P=0.032N*  |
|LOGISTIC REGRESSION    |  P=0.252N     P=0.758N     P=0.692N     P=0.400N   |  P=0.197N     P=0.473N     P=0.227N     P=0.277N   |
|COCHRAN-ARMITAGE       |  P=0.321N                                          |  P=0.169N                                          |
|FISHER EXACT           |               P=0.748      P=0.752N     P=0.500N   |               P=0.500N     P=0.248N     P=0.248N   |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 02                                                 PAGE  16
                           STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1,2,3-TRICHLOROPROPANE
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Lymphoma And Histiocytic Sarcoma                                                                                  |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  6/60 (10%)   5/59 (8%)    1/60 (2%)    1/60 (2%)  | 17/60 (28%)   7/60 (12%)   3/60 (5%)    3/60 (5%)  |
|ADJUSTED (b)           |  13.5%        20.9%         3.3%         1.7%      |  38.4%        41.2%        26.7%        11.7%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     0/6 (0%)     0/4 (0%)   |  0/10 (0%)    0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          0/10 (0%)    0/0        |  0/0          0/0          2/9 (22%)    0/0        |
|TERMINAL (d)           |  4/42 (10%)   2/18 (11%)   0/0          0/0        | 14/41 (34%)   4/13 (31%)   0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   495          620          540          322       |   480          600          608          419       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.296      P=0.321      P=0.685      P=0.675    |  P=0.003 **   P=0.465      P=0.585      P=0.107    |
|INCIDENTAL TUMOR       |  P=0.279N     P=0.398N     P=0.355N     P=0.454N   |  P=0.629      P=0.222N     P=0.200N     P=0.286N   |
|LOGISTIC REGRESSION    |  P=0.254N     P=0.614      P=0.211N     P=0.168N   |  P=0.235      P=0.216N     P=0.588N     P=0.613N   |
|COCHRAN-ARMITAGE       |  P=0.033N*                                         |  P=0.002N**                                        |
|FISHER EXACT           |               P=0.512N     P=0.057N     P=0.057N   |               P=0.019N*    P<0.001N**   P<0.001N** |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Lymphoma: Histiocytic, Lymphocytic, Mixed,                                                                        |
|     NOS, or Undifferentiated Cell Type                                                                                          |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  5/60 (8%)    4/59 (7%)    0/60 (0%)    1/60 (2%)  | 15/60 (25%)   6/60 (10%)   3/60 (5%)    3/60 (5%)  |
|ADJUSTED (b)           |  11.6%        18.5%         0.0%         1.7%      |  34.8%        39.5%        26.7%        11.7%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     0/8 (0%)     0/6 (0%)     0/4 (0%)   |  0/10 (0%)    0/10 (0%)    0/9 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          0/6 (0%)   |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0          0/10 (0%)    0/0        |  0/0          0/0          2/9 (22%)    0/0        |
|TERMINAL (d)           |  4/42 (10%)   2/18 (11%)   0/0          0/0        | 13/41 (32%)   4/13 (31%)   0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   682          660          ---          322       |   691          705          608          419       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.155      P=0.318        (e)        P=0.503    |  P<0.001 **   P=0.426      P=0.235      P=0.036 *  |
|INCIDENTAL TUMOR       |  P=0.528      P=0.553N       (e)        P=0.638    |  P=0.189      P=0.361N     P=0.965      P=0.786    |
|LOGISTIC REGRESSION    |  P=0.670N     P=0.518      P=0.786N     P=0.517N   |  P=0.031 *    P=0.558N     P=0.297      P=0.511    |
|COCHRAN-ARMITAGE       |  P=0.062N                                          |  P=0.006N**                                        |
|FISHER EXACT           |               P=0.511N     P=0.029N*    P=0.103N   |               P=0.026N*    P=0.002N**   P=0.002N** |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 02                                                 PAGE  17
                           STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1,2,3-TRICHLOROPROPANE
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Benign Tumors                                                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 20/60 (33%)  49/59 (83%)  46/60 (77%)  50/60 (83%) | 20/60 (33%)  36/60 (60%)  40/60 (67%)  53/60 (88%) |
|ADJUSTED (b)           |  41.2%       100.0%       100.0%       100.0%      |  43.5%        96.8%        96.7%       100.0%      |
|INTERIM SACRIFICE 1 (c)|  1/8 (13%)    7/8 (88%)    4/6 (67%)    3/4 (75%)  |  3/10 (30%)   5/10 (50%)   9/9 (100%)   5/5 (100%) |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          6/6 (100%) |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          9/9 (100%) |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0         10/10 (100%)  0/0        |  0/0          0/0          9/9 (100%)   0/0        |
|TERMINAL (d)           | 14/42 (33%)  18/18 (100%)  0/0          0/0        | 16/41 (39%)  12/13 (92%)   0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   457 (I)      424          388          322       |   461 (I)      461 (I)      312          377       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P=0.004 **   P<0.001 **   P=0.018 *    P=0.002 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|LOGISTIC REGRESSION    |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P<0.001 **                                        |
|FISHER EXACT           |               P<0.001 **   P<0.001 **   P<0.001 ** |               P=0.003 **   P<0.001 **   P<0.001 ** |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Tumors                                                                                                            |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 15/60 (25%)  44/59 (75%)  56/60 (93%)  58/60 (97%) | 23/60 (38%)  48/60 (80%)  55/60 (92%)  58/60 (97%) |
|ADJUSTED (b)           |  31.8%        89.6%       100.0%        96.7%      |  48.5%        97.9%       100.0%       100.0%      |
|INTERIM SACRIFICE 1 (c)|  0/8 (0%)     1/8 (13%)    4/6 (67%)    4/4 (100%) |  0/10 (0%)    1/10 (10%)   6/9 (67%)    5/5 (100%) |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          6/6 (100%) |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          7/9 (78%)  |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0         10/10 (100%)  0/0        |  0/0          0/0          9/9 (100%)   0/0        |
|TERMINAL (d)           | 10/42 (24%)  13/18 (72%)   0/0          0/0        | 17/41 (41%)  12/13 (92%)   0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   495          424          385          322       |    68          414          312          295       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|LOGISTIC REGRESSION    |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P<0.001 **                                        |
|FISHER EXACT           |               P<0.001 **   P<0.001 **   P<0.001 ** |               P<0.001 **   P<0.001 **   P<0.001 ** |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 02                                                 PAGE  18
                           STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1,2,3-TRICHLOROPROPANE
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |   0 MG/KG      6 MG/KG      20 MG/KG     60 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant and Benign Tumors                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 30/60 (50%)  57/59 (97%)  59/60 (98%)  60/60 (100%)| 39/60 (65%)  54/60 (90%)  59/60 (98%)  60/60 (100%)|
|ADJUSTED (b)           |  58.7%       100.0%       100.0%       100.0%      |  76.2%       100.0%       100.0%       100.0%      |
|INTERIM SACRIFICE 1 (c)|  1/8 (13%)    7/8 (88%)    5/6 (83%)    4/4 (100%) |  3/10 (30%)   6/10 (60%)   9/9 (100%)   5/5 (100%) |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/0          0/0          0/0          6/6 (100%) |
|INTERIM SACRIFICE 3 (c)|  0/0          0/0          0/0          9/9 (100%) |                                                    |
|INTERIM SACRIFICE 4 (c)|  0/0          0/0         10/10 (100%)  0/0        |  0/0          0/0          9/9 (100%)   0/0        |
|TERMINAL (d)           | 21/42 (50%)  18/18 (100%)  0/0          0/0        | 29/41 (71%)  13/13 (100%)  0/0          0/0        |
|FIRST INCIDENCE (DAYS) |   457 (I)      424          385          322       |    68          414          312          295       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P<0.001 **   P<0.001 **   P=0.003 **   P<0.001 ** |  P<0.001 **   P=0.011 *    P<0.001 **   P<0.001 ** |
|LOGISTIC REGRESSION    |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P<0.001 **                                        |
|FISHER EXACT           |               P<0.001 **   P<0.001 **   P<0.001 ** |               P<0.001 **   P<0.001 **   P<0.001 ** |
|=================================================================================================================================|
 
 (a)  Number of tumor-bearing animals / number of animals examined at site.
 (b)  Kaplan-Meier estimated lifetime tumor incidence after adjustment for 
      intercurrent mortality.
 (c)  Observed incidence at interim kill.
          Male interim sacrifices(days):   457  554  623
          Female interim sacrifices(days): 461  511  620
 (d)  Observed incidence at terminal kill.
 (f)  Beneath the control incidence are the P-values associated with the trend 
      test.  Beneath the dosed group incidence are the P-values corresponding to 
      pairwise comparisons between the controls and that dosed group.  The life
      table analysis regards tumors in animals dying  prior to terminal kill as
      being (directly or indirectly) the cause of death.  The incidental tumor 
      test regards these lesions as non-fatal.  Logistic regression is an alternative
      method for analyzing the incidence of non-fatal tumors.  The Cochran-Armitage
      and Fisher's exact tests compare directly the overall incidence rates.  
      For all tests, a negative trend is indicated by "N".
 (e)  Value of Statistic cannot be computed. 
 (I)  Interim sacrifice
 (T)  Terminal sacrifice
  #   Tumor rates based on number of animals necropsied.
 
  *   To the right of any statistical result, indicates significance at (P<=0.05).
  **  To the right of any statistical result, indicates significance at (P<=0.01).

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