National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/3370

TDMS Study 05114-02 Pathology Tables

NTP           STATISTICAL ANALYSIS OF SURVIVAL DATA            REPORT: PEIRPT11 
LAB: Battelle Northwest                                        DATE: 09/15/94   
EXP: 05114            HEXACHLOROCYCLOPENTADIENE (HCCPD)        TIME: 07:15:07   
TEST: 02              CAGES FROM 0000 TO LAST CAGE             PAGE:   1        
TEST TYPE: CHRONIC          ROUTE: RESPIRATORY EXPOSURE WHOLE  NTP C#: 55607B   
CONT: N01ES2800301                                             CAS: 77-47-4     
PATHOLOGIST: E. GIDDENS                      HAILEY, JAMESR.                    
--------------------------------------------------------------------------------
                                                                                
                                                                                
                                                                                


REASONS FOR REMOVAL:                                                            
                                                                                
ALL                                                                             
                                                                                
                                                                                
REMOVAL DATE RANGE:                                                             


ALL                                                                             
                                                                                
TREATMENT GROUPS:                                                               
                                                                                
INCLUDE ALL                                                                     

NTP           STATISTICAL ANALYSIS OF SURVIVAL DATA            REPORT: PEIRPT11
LAB: Battelle Northwest                                        DATE: 09/15/94  
EXP: 05114            HEXACHLOROCYCLOPENTADIENE (HCCPD)        TIME: 07:15:07  
TEST: 02              CAGES FROM 0000 TO LAST CAGE                             
TEST TYPE: CHRONIC          ROUTE: RESPIRATORY EXPOSURE WHOLE  NTP C#: 55607B  
CONT: N01ES2800301                                             CAS: 77-47-4    
PATHOLOGIST: E. GIDDENS                      HAILEY, JAMESR.                   

                         HEXACHLOROCYCLOPENTADIENE (HCCPD)


                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                     MALE MICE
                       FIRST TERMINAL SACRIFICE AT 729 DAYS
                                                          


                          INDIVIDUAL SURVIVAL TIMES (DAYS)
 ==================================================================================
 |                                DOSE = CONTROL                                  |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 90     UNCENSORED DEATHS 14     CENSORED DEATHS 41   TERMINAL 35        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      264   464   554   607   626   627   643   648   649   681                 |
 |      682   689   711   717                                                     |
 |  CENSORED DEATHS                                                               |
 |      37    183   183   183   183   183   183   183   183   183                 |
 |      183   232   232   232   232   232   232   232   232   232                 |
 |      232   295   295   295   295   295   295   295   295   295                 |
 |      295   459   459   459   459   459   459   459   459   459                 |
 |      459                                                                       |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                                DOSE = 0.01 PPM                                 |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 60     UNCENSORED DEATHS 15     CENSORED DEATHS 12   TERMINAL 33        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      425   443   451   516   557   570   606   617   682   687                 |
 |      689   697   711   714   725                                               |
 |  CENSORED DEATHS                                                               |
 |      319   459   459   459   459   459   459   459   459   459                 |
 |      459   655                                                                 |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                                DOSE = 0.05 PPM                                 |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 60     UNCENSORED DEATHS 8      CENSORED DEATHS 10   TERMINAL 42        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      529   607   607   618   620   699   715   718                             |
 |  CENSORED DEATHS                                                               |
 |      459   459   459   459   459   459   459   459   459   459                 |
 |                                                                                |
 ==================================================================================

                         HEXACHLOROCYCLOPENTADIENE (HCCPD)


                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                     MALE MICE
                       FIRST TERMINAL SACRIFICE AT 729 DAYS
                                                          


                          INDIVIDUAL SURVIVAL TIMES (DAYS)
 ==================================================================================
 |                                DOSE = 0.2  PPM                                 |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 60     UNCENSORED DEATHS 16     CENSORED DEATHS 10   TERMINAL 34        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      393   435   460   481   514   522   542   555   617   642                 |
 |      664   683   707   715   717   726                                         |
 |  CENSORED DEATHS                                                               |
 |      459   459   459   459   459   459   459   459   459   459                 |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                          DOSE = 0.2  PPMSE33                                   |
 |                       ----------------------------                             |
 |                                                                                |
 |  TOTAL 80     UNCENSORED DEATHS 14     CENSORED DEATHS 31   TERMINAL 35        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      521   542   545   616   626   626   628   634   641   653                 |
 |      661   696   717   718                                                     |
 |  CENSORED DEATHS                                                               |
 |      220   232   232   232   232   232   232   232   232   232                 |
 |      232   295   295   295   295   295   295   295   295   295                 |
 |      295   459   459   459   459   459   459   459   459   459                 |
 |      459                                                                       |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                          DOSE = 0.2  PPMSE65                                   |
 |                       ----------------------------                             |
 |                                                                                |
 |  TOTAL 50     UNCENSORED DEATHS 16     CENSORED DEATHS 1    TERMINAL 33        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      255   410   452   499   526   557   618   622   623   647                 |
 |      653   654   656   671   704   706                                         |
 |  CENSORED DEATHS                                                               |
 |      323                                                                       |
 |                                                                                |
 ==================================================================================

                         HEXACHLOROCYCLOPENTADIENE (HCCPD)


                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                     MALE MICE
                       FIRST TERMINAL SACRIFICE AT 729 DAYS
                                                          


                          INDIVIDUAL SURVIVAL TIMES (DAYS)
 ==================================================================================
 |                          DOSE = 0.5  PPMSE26                                   |
 |                       ----------------------------                             |
 |                                                                                |
 |  TOTAL 90     UNCENSORED DEATHS 9      CENSORED DEATHS 40   TERMINAL 41        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      381   494   505   570   612   675   703   703   725                       |
 |  CENSORED DEATHS                                                               |
 |      183   183   183   183   183   183   183   183   183   183                 |
 |      232   232   232   232   232   232   232   232   232   232                 |
 |      295   295   295   295   295   295   295   295   295   295                 |
 |      458   458   458   458   458   458   458   458   458   458                 |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                          DOSE = 0.5  PPMSE42                                   |
 |                       ----------------------------                             |
 |                                                                                |
 |  TOTAL 70     UNCENSORED DEATHS 17     CENSORED DEATHS 20   TERMINAL 33        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      219   245   247   257   262   274   291   292   320   395                 |
 |      487   601   626   647   652   661   717                                   |
 |  CENSORED DEATHS                                                               |
 |      295   295   295   295   295   295   295   295   295   295                 |
 |      458   458   458   458   458   458   458   458   458   458                 |
 |                                                                                |
 ==================================================================================

                         HEXACHLOROCYCLOPENTADIENE (HCCPD)


                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                     MALE MICE
                       FIRST TERMINAL SACRIFICE AT 729 DAYS


 ==================================================================================
 |               KAPLAN-MEIER SURVIVAL PROBABILITY ESTIMATES (%)                  |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |    DOSE                               TIME (DAYS)                              |
 |                 90   180   270   365   425   485   545   605   665   729 (A)   |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |           |                                                                    |
 |CONTROL    |   100.0 100.0  98.6  98.6  98.6  96.5  96.5  94.4  82.1  71.9      |
 |           |                                                                    |
 |           |                                                                    |
 |0.01 PPM   |   100.0 100.0 100.0 100.0  98.3  94.9  92.9  88.7  84.6  69.8      |
 |           |                                                                    |
 |           |                                                                    |
 |0.05 PPM   |   100.0 100.0 100.0 100.0 100.0 100.0  98.0  98.0  90.0  84.0      |
 |           |                                                                    |
 |           |                                                                    |
 |0.2  PPM   |   100.0 100.0 100.0 100.0  98.3  92.6  86.6  84.6  78.5  68.5      |
 |           |                                                                    |
 |           |                                                                    |
 |0.2        |   100.0 100.0 100.0 100.0 100.0 100.0  93.9  93.9  77.6  71.4      |
 |PPMSE33    |                                                                    |
 |           |                                                                    |
 |0.2        |   100.0 100.0  98.0  98.0  96.0  93.9  89.8  87.8  73.5  67.4      |
 |PPMSE65    |                                                                    |
 |           |                                                                    |
 |0.5        |   100.0 100.0 100.0 100.0  98.3  98.3  94.3  92.3  90.3  82.3      |
 |PPMSE26    |                                                                    |
 |           |                                                                    |
 |0.5        |   100.0 100.0  92.9  86.9  85.2  85.2  83.0  80.9  72.4  70.3      |
 |PPMSE42    |                                                                    |
 |           |                                                                    |
 ==================================================================================

                         HEXACHLOROCYCLOPENTADIENE (HCCPD)


                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                     MALE MICE
                       FIRST TERMINAL SACRIFICE AT 729 DAYS


 ===============================================================================================================================
 |                                               SURVIVAL SUMMARY STATISTICS                                                   |
 |                                                                                                                             |
 |                                                                                                                             |
 |-----------------------------------------------------------------------------------------------------------------------------|
 |                                                                        DOSE                                                 |
 |                                                                                                                             |
 |                                CONTROL     0.01 PPM    0.05 PPM    0.2  PPM    0.2         0.2         0.5         0.5      |
 |                                                                                PPMSE33     PPMSE65     PPMSE26     PPMSE42  |
 |-----------------------------------------------------------------------------------------------------------------------------|
 |SURVIVAL AT END OF STUDY|       71.9%       69.8%       84.0%       68.5%       71.4%       67.4%       82.3%       70.3%    |
 |(KAPLAN-MEIER)          |                                                                                                    |
 |                        |                                                                                                    |
 |SIGNIFICANCE (B)        |       P=0.901     P=0.936     P=0.204N    P=0.794     P=1.000     P=0.652     P=0.311N    P=0.500  |
 |(LIFE TABLE)            |                                                                                                    |
 |                        |                                                                                                    |
 |MEAN DAY OF             |       611.6       606.0       639.1       585.8       630.3       578.3       596.4       423.1    |
 |NATURAL DEATHS (C)      |       (32.0)      (27.6)      (23.4)      (27.9)      (16.3)      (30.7)      (39.4)      (44.9)   |
 |(STANDARD ERROR)        |                                                                                                    |
 |                        |                                                                                                    |
 |MEAN LIFE SPAN (D)      |       508.9       645.2       672.0       645.8       555.2       672.7       521.5       554.0    |
 |(STANDARD ERROR)        |       (23.9)      (15.7)      (13.3)      (15.3)      (22.2)      (15.6)      (23.6)      (23.3)   |
 |                        |                                                                                                    |
 ===============================================================================================================================
                                    


       (A)  FIRST TERMINAL SACRIFICE
       (B)  THE FIRST ENTRY IS THE TREND TEST (TARONE, 1975) RESULT.
            SUBSEQUENT ENTRIES ARE THE RESULTS OF PAIRWISE TESTS
            (COX,1972).  NEGATIVE TRENDS ARE INDICATED BY "N".
       (C)  MEAN OF ALL UNCENSORED DEATHS PRIOR TO TERMINAL SACRIFICE
       (D)  MEAN OF ALL DEATHS (UNCENSORED, CENSORED, TERMINAL SACRIFICE)

                         HEXACHLOROCYCLOPENTADIENE (HCCPD)


                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                    FEMALE MICE
                       FIRST TERMINAL SACRIFICE AT 736 DAYS
                                                          


                          INDIVIDUAL SURVIVAL TIMES (DAYS)
 ==================================================================================
 |                                DOSE = CONTROL                                  |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 60     UNCENSORED DEATHS 18     CENSORED DEATHS 11   TERMINAL 31        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      249   396   483   520   584   589   598   620   629   633                 |
 |      634   657   660   672   687   691   693   716                             |
 |  CENSORED DEATHS                                                               |
 |      157   458   458   458   458   458   458   458   458   458                 |
 |      458                                                                       |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                                DOSE = 0.01 PPM                                 |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 60     UNCENSORED DEATHS 18     CENSORED DEATHS 10   TERMINAL 32        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      340   503   555   558   572   576   577   593   629   633                 |
 |      646   660   660   668   673   680   688   730                             |
 |  CENSORED DEATHS                                                               |
 |      458   458   458   458   458   458   458   458   458   458                 |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                                DOSE = 0.05 PPM                                 |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 60     UNCENSORED DEATHS 19     CENSORED DEATHS 11   TERMINAL 30        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      74    411   554   554   574   575   592   604   625   635                 |
 |      643   654   660   674   674   688   706   715   730                       |
 |  CENSORED DEATHS                                                               |
 |      458   458   458   458   458   458   458   458   458   458                 |
 |      718                                                                       |
 |                                                                                |
 ==================================================================================

                         HEXACHLOROCYCLOPENTADIENE (HCCPD)


                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                    FEMALE MICE
                       FIRST TERMINAL SACRIFICE AT 736 DAYS
                                                          


                          INDIVIDUAL SURVIVAL TIMES (DAYS)
 ==================================================================================
 |                                DOSE = 0.2   PPM                                |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 60     UNCENSORED DEATHS 28     CENSORED DEATHS 11   TERMINAL 21        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      400   418   466   496   498   500   516   519   528   546                 |
 |      554   570   606   610   610   611   614   618   619   621                 |
 |      673   674   690   695   696   724   730   730                             |
 |  CENSORED DEATHS                                                               |
 |      22    458   458   458   458   458   458   458   458   458                 |
 |      458                                                                       |
 |                                                                                |
 ==================================================================================

                         HEXACHLOROCYCLOPENTADIENE (HCCPD)


                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                    FEMALE MICE
                       FIRST TERMINAL SACRIFICE AT 736 DAYS


 ==================================================================================
 |               KAPLAN-MEIER SURVIVAL PROBABILITY ESTIMATES (%)                  |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |    DOSE                               TIME (DAYS)                              |
 |                 90   180   270   365   425   485   545   605   665   736 (A)   |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |           |                                                                    |
 |CONTROL    |   100.0 100.0  98.3  98.3  96.6  94.6  92.5  86.3  74.0  63.7      |
 |           |                                                                    |
 |           |                                                                    |
 |0.01 PPM   |   100.0 100.0 100.0  98.3  98.3  98.3  96.3  84.3  74.3  64.2      |
 |           |                                                                    |
 |           |                                                                    |
 |0.05 PPM   |    98.3  98.3  98.3  98.3  96.7  96.7  96.7  84.6  74.5  62.4      |
 |           |                                                                    |
 |           |                                                                    |
 |0.2   PPM  |   100.0 100.0 100.0 100.0  96.6  94.6  82.2  76.1  59.6  43.2      |
 |           |                                                                    |
 |           |                                                                    |
 ==================================================================================
 ==================================================================================
 |                           SURVIVAL SUMMARY STATISTICS                          |
 |                                                                                |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                                                  DOSE                          |
 |                                                                                |
 |                                CONTROL      0.01 PPM     0.05 PPM     0.2   PPM|
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |SURVIVAL AT END OF STUDY|       63.7%        64.2%        62.4%        43.2%    |
 |(KAPLAN-MEIER)          |                                                       |
 |                        |                                                       |
 |SIGNIFICANCE (B)        |       P=0.010      P=1.000N     P=1.000      P=0.053  |
 |(LIFE TABLE)            |                                                       |
 |                        |                                                       |
 |MEAN DAY OF             |       595.1        607.8        596.9        590.4    |
 |NATURAL DEATHS (C)      |       (27.9)       (20.9)       (33.7)       (17.5)   |
 |(STANDARD ERROR)        |                                                       |
 |                        |                                                       |
 |MEAN LIFE SPAN (D)      |       637.7        651.2        645.3        609.8    |
 |(STANDARD ERROR)        |       (17.8)       (14.8)       (17.1)       (18.1)   |
 |                        |                                                       |
 ==================================================================================
                                    


       (A)  FIRST TERMINAL SACRIFICE
       (B)  THE FIRST ENTRY IS THE TREND TEST (TARONE, 1975) RESULT.
            SUBSEQUENT ENTRIES ARE THE RESULTS OF PAIRWISE TESTS
            (COX,1972).  NEGATIVE TRENDS ARE INDICATED BY "N".
       (C)  MEAN OF ALL UNCENSORED DEATHS PRIOR TO TERMINAL SACRIFICE
       (D)  MEAN OF ALL DEATHS (UNCENSORED, CENSORED, TERMINAL SACRIFICE)

                                                 HEXACHLOROCYCLOPENTADIENE (HCCPD)
                                                    KAPLAN-MEIER SURVIVAL CURVES
                                                        CONTROL          = 1
                                                        0.01 PPM         = 2
                                                        0.05 PPM         = 3
                                                        0.2  PPM         = 4
                                                        0.2  PPMSE33     = 5
                                                        0.2  PPMSE65     = 6
                                                        0.5  PPMSE26     = 7
                                                        0.5  PPMSE42     = 8

------------------------------------------------------ SPECIES=MICE SEX=MALE -------------------------------------------------------

                                         Plot of SURVIVAL*WEEK.  Symbol is value of DOSE.

      |
      |
  100 + 1                                  8     61                  7 4   2               53
      |                                    8   8  1                  7 4   2 42   1   7
      |                                        886                       6                 53 5         3
      |                                         88                       6   42 241   7 7       1
   95 +                                                                         24  4    2      1       1
      |                                          88                             6      67     5   7     353
P     |                                           8 8                               4  6 2  6   2 7     17 1
E     |                                             8 8                                  4 4    2 2      5 5
R  90 +                                                                                     6   6        73        7  3
C     |                                               8    8                               4  4   2     2  1 1     7   7
E     |                                                                                         6         6           3  3
N     |                                                    8           8                      4 4       2 25511
T  85 +                                                                8             8          4         2         2
      |                                                                                                     55         7 37 3
S     |                                                                              8                 8  4  41     12    7 7
U     |                                                                                                8  68 565
R  80 +                                                                                                      465 4
V     |                                                                                                    8  8  4  422
I     |                                                                                                          5  115
V     |                                                                                                       88    41241
A  75 +                                                                                                        8 8    54 4
L     |                                                                                                        6  6     11
      |                                                                                                          8      282
      |                                                                                                           6    6 1  1
   70 +                                                                                                                  42 2
      |                                                                                                                   4 4
      |                                                                                                                6    6
      |
   65 +
      |
      --+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+--
        0         10         20         30         40         50         60         70         80         90         100        110

                                                                   WEEK

NOTE: 20 obs hidden.

                                                 HEXACHLOROCYCLOPENTADIENE (HCCPD)
                                                    KAPLAN-MEIER SURVIVAL CURVES
                                                        CONTROL          = 1
                                                        0.01 PPM         = 2
                                                        0.05 PPM         = 3
                                                        0.2   PPM        = 4

----------------------------------------------------- SPECIES=MICE SEX=FEMALE ------------------------------------------------------

                                         Plot of SURVIVAL*WEEK.  Symbol is value of DOSE.

      |
      |
  100 + 1           3                           1             2         4
      |             3                           1             2        1434       4 1  2        2
      |                                                                           4 1 4    1
      |                                                                               44   1    2 2 1
P  90 +                                                                                           221 1
E     |                                                                                4 4 4       2  113 1
R     |                                                                                    44         2 3 11
C     |                                                                                     4 4            11
E  80 +                                                                                       4 4           232
N     |                                                                                         4 4         1321 2
T     |                                                                                           4     44     1 113
      |                                                                                                           12 1
S  70 +                                                                                                            223
U     |                                                                                                             22 3 3
R     |                                                                                                  44          1   1  22
V     |                                                                                                                  1  31
I  60 +                                                                                                   4        4
V     |
A     |                                                                                                            4 4
L     |                                                                                                              44
   50 +                                                                                                               4   4
      |                                                                                                                   4 4
      |
      |                                                                                                                     44
   40 +
      |
      --+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+--
        0         10         20         30         40         50         60         70         80         90         100        110

                                                                   WEEK

NOTE: 42 obs hidden.
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.