National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/2940

TDMS Study 05134-02 Pathology Tables


                              INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)
                                                     1,3 BUTADIENE (BUTADIENE)


NTP Experiment-Test: 05134-02                                                                                     Report: PEIRPT05
Study Type: CHRONIC                                                                                               Date: 09/17/94
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59




       Facility:  Battelle Northwest

       Chemical CAS #:  106-99-0

       Lock Date:  None

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All
































a  Number of animals examined microscopically at site and number of animals with lesion

                                                             Page   1

NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        CONTROL      6.25 PPM     20 PPM       62.5 PPM     200 PPM      625 PPM         
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
  Animals Initially in Study                          70           70           70           70           70           90           
  Scheduled Sacrifice                                 20           20           20           20           20           10           
  Early Deaths                                                                                                                      
    Moribund                                          10           10           14           31           37           46           
    Dead                                               3            7           11            8           12           33           
    Accident                                                                     1                         1            1           
  Survivors                                                                                                                         
    Terminal Sacrifice                                37           33           24           11                                     
  Animals Examined Microscopically                    70           70           70           70           70           90           
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Gallbladder                                        (66)         (44)         (41)         (43)         (49)         (64)         
   Intestine Large, Cecum                             (68)         (46)         (46)         (47)         (57)         (78)         
      Histiocytic Sarcoma                                                                                               1 (1%)      
   Intestine Small, Duodenum                          (66)         (45)         (44)         (46)         (53)         (72)         
      Adenoma                                                       1 (2%)                                                          
      Carcinoma, Multiple                                                                     1 (2%)                                
      Histiocytic Sarcoma                                                                                  1 (2%)                   
      Leiomyosarcoma                                                                          1 (2%)                                
   Intestine Small, Ileum                             (68)         (46)         (43)         (47)         (55)         (75)         
      Carcinoma                                                     1 (2%)                                                          
      Leiomyosarcoma, Metastatic, Stomach                                                                  1 (2%)                   
   Intestine Small, Jejunum                           (67)         (45)         (44)         (47)         (54)         (70)         
      Carcinoma                                                     1 (2%)                                                          
      Histiocytic Sarcoma                                                                                  1 (2%)                   
      Leiomyosarcoma, Metastatic, Stomach                                                                  1 (2%)                   
   Liver                                              (69)         (52)         (51)         (61)         (61)         (90)         
      Adenocarcinoma, Metastatic, Mammary Gland                                                                         1 (1%)      
      Carcinoma, Metastatic, Parathyroid Gland                                   1 (2%)                                             
      Hemangiosarcoma                                  1 (1%)                                 1 (2%)                                
      Hemangiosarcoma, Multiple                                                                            1 (2%)       1 (1%)      
      Hemangiosarcoma, Metastatic, Heart                                                                  10 (16%)     20 (22%)     
      Hemangiosarcoma, Metastatic, Skin                                                       1 (2%)                    1 (1%)      
      Hepatocellular Carcinoma                         4 (6%)       5 (10%)      7 (14%)      9 (15%)      8 (13%)      1 (1%)      
      Hepatocellular Carcinoma, Multiple                            1 (2%)       1 (2%)                    1 (2%)                   
      Hepatocellular Adenoma                          11 (16%)     10 (19%)      8 (16%)     10 (16%)     10 (16%)      2 (2%)      
      Hepatocellular Adenoma, Multiple                 1 (1%)       1 (2%)       1 (2%)       5 (8%)       5 (8%)                   
      Histiocytic Sarcoma                              3 (4%)       2 (4%)       6 (12%)      3 (5%)       7 (11%)      4 (4%)      
      Leiomyosarcoma, Metastatic, Intestine Small                                             1 (2%)                                
      Leiomyosarcoma, Metastatic, Stomach                                                                  1 (2%)                   
      Sarcoma, Metastatic, Mammary Gland                                                                                1 (1%)      
                                                             Page   2                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        CONTROL      6.25 PPM     20 PPM       62.5 PPM     200 PPM      625 PPM         
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - cont                                                                                                            
      Squamous Cell Carcinoma, Metastatic, Stomach                                                                      2 (2%)      
   Mesentery                                          (12)         (13)         (16)         (9)          (15)         (6)          
      Histiocytic Sarcoma                                                                     1 (11%)                               
      Sarcoma, Metastatic, Mammary Gland                                                                                1 (17%)     
      Fat, Hemangiosarcoma, Metastatic, Heart                                                              2 (13%)      1 (17%)     
      Fat, Hemangiosarcoma, Metastatic, Spleen                                                                          1 (17%)     
      Fat, Histiocytic Sarcoma                                                   2 (13%)                   2 (13%)                  
      Fat, Leiomyosarcoma, Metastatic, Intestine                                                                                    
          Small                                                                               1 (11%)                               
      Fat, Leiomyosarcoma, Metastatic, Stomach                                                             1 (7%)                   
      Fat, Sarcoma, Metastatic, Skin                   1 (8%)                                 1 (11%)                               
      Fat, Sarcoma, Metastatic, Stomach                                                                                 1 (17%)     
      Fat, Sarcoma, Metastatic, Uterus                              1 (8%)                                                          
      Fat, Squamous Cell Carcinoma, Metastatic,                                                                                     
           Stomach                                                                                                      1 (17%)     
   Pancreas                                           (69)         (49)         (47)         (48)         (59)         (88)         
      Sarcoma, Metastatic, Mammary Gland                                                                                1 (1%)      
      Sarcoma, Metastatic, Skin                        1 (1%)                                                                       
      Acinus, Carcinoma, Metastatic, Ovary                                                    1 (2%)                                
      Acinus, Hemangioma                                                                      1 (2%)                                
      Acinus, Histiocytic Sarcoma                      2 (3%)                                 2 (4%)                                
      Acinus, Leiomyosarcoma, Metastatic,                                                                                           
          Intestine Small                                                                     1 (2%)                                
      Acinus, Leiomyosarcoma, Metastatic, Stomach                                                          1 (2%)                   
      Acinus, Sarcoma, Metastatic, Skin                                                       1 (2%)                                
      Acinus, Squamous Cell Carcinoma, Metastatic,                                                                                  
           Stomach                                                                                                      2 (2%)      
   Salivary Glands                                    (70)         (49)         (50)         (49)         (60)         (89)         
   Stomach, Forestomach                               (70)         (50)         (57)         (68)         (70)         (89)         
      Histiocytic Sarcoma                                                                     1 (1%)                                
      Leiomyoma                                        1 (1%)                                                                       
      Leiomyosarcoma                                                                                       1 (1%)                   
      Sarcoma                                                                                                           1 (1%)      
      Sarcoma, Metastatic, Mammary Gland                                                                                1 (1%)      
      Squamous Cell Carcinoma                                                    1 (2%)       1 (1%)       1 (1%)       7 (8%)      
      Squamous Cell Papilloma                                                    2 (4%)       2 (3%)       4 (6%)      15 (17%)     
      Squamous Cell Papilloma, Multiple                                                                    1 (1%)       1 (1%)      
   Stomach, Glandular                                 (69)         (49)         (58)         (68)         (68)         (86)         
      Histiocytic Sarcoma                              1 (1%)                                                           1 (1%)      
      Sarcoma, Metastatic, Mammary Gland                                                                                1 (1%)      
      Squamous Cell Carcinoma, Metastatic, Stomach                                                                      3 (3%)      
   Tongue                                                                                    (1)                                    
      Squamous Cell Carcinoma                                                                 1 (100%)                              
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                             Page   3                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        CONTROL      6.25 PPM     20 PPM       62.5 PPM     200 PPM      625 PPM         
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (70)         (50)         (50)         (59)         (70)         (90)         
      Adenocarcinoma, Metastatic, Lung                              1 (2%)                                                          
      Hemangiosarcoma                                                                         1 (2%)      22 (31%)     25 (28%)     
      Histiocytic Sarcoma                              1 (1%)                    2 (4%)       1 (2%)                    1 (1%)      
      Sarcoma, Metastatic, Mammary Gland                                                                                1 (1%)      
      Pericardium, Leiomyosarcoma, Metastatic,                                                                                      
           Uterus                                                                1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Gland, Cortex                              (70)         (49)         (48)         (49)         (60)         (88)         
      Adenocarcinoma, Metastatic, Mammary Gland                                                                         1 (1%)      
      Adenoma                                                                                              3 (5%)                   
      Hepatocellular Carcinoma, Metastatic, Liver                   1 (2%)                                                          
      Histiocytic Sarcoma                              2 (3%)       1 (2%)       1 (2%)                    3 (5%)       2 (2%)      
      Leiomyosarcoma, Metastatic, Stomach                                                                  1 (2%)                   
      Sarcoma, Metastatic, Skin                                                                            1 (2%)                   
      Sarcoma, Metastatic, Stomach                                                                                      1 (1%)      
      Capsule, Adenoma                                 1 (1%)                                                                       
   Adrenal Gland, Medulla                             (70)         (48)         (47)         (45)         (60)         (86)         
      Adenocarcinoma, Metastatic, Mammary Gland                                                                         1 (1%)      
      Histiocytic Sarcoma                              1 (1%)                                              1 (2%)                   
      Leiomyosarcoma, Metastatic, Stomach                                                                  1 (2%)                   
      Pheochromocytoma Malignant                                                 1 (2%)                                             
      Pheochromocytoma Benign                                                    1 (2%)                    2 (3%)                   
      Sarcoma, Metastatic, Skin                                                                            1 (2%)                   
   Islets, Pancreatic                                 (69)         (49)         (46)         (47)         (59)         (88)         
      Adenoma                                                                                 1 (2%)       2 (3%)                   
   Parathyroid Gland                                  (57)         (40)         (33)         (39)         (47)         (77)         
      Carcinoma                                                                  1 (3%)                                             
   Pituitary Gland                                    (70)         (49)         (48)         (47)         (58)         (80)         
      Histiocytic Sarcoma                              1 (1%)                                                                       
      Pars Distalis, Adenoma                           7 (10%)     12 (24%)      5 (10%)      4 (9%)                                
   Thyroid Gland                                      (69)         (49)         (49)         (49)         (59)         (88)         
      Carcinoma, Metastatic, Parathyroid Gland                                   1 (2%)                                             
      Follicular Cell, Adenoma                         1 (1%)       1 (2%)       1 (2%)       2 (4%)       2 (3%)       1 (1%)      
      Follicular Cell, Carcinoma                                    1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   Tissue NOS                                                                                (1)                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                             Page   4                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        CONTROL      6.25 PPM     20 PPM       62.5 PPM     200 PPM      625 PPM         
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Ovary                                              (69)         (59)         (58)         (70)         (70)         (89)         
      Adenoma                                          2 (3%)       4 (7%)       1 (2%)       3 (4%)       1 (1%)       1 (1%)      
      Carcinoma                                                                               1 (1%)                                
      Granular Cell Tumor Benign                                    1 (2%)                                                          
      Granulosa Cell Tumor Malignant                                                          3 (4%)       3 (4%)                   
      Granulosa Cell Tumor Benign                      1 (1%)                    1 (2%)       7 (10%)      9 (13%)      7 (8%)      
      Hemangioma                                       1 (1%)                                 2 (3%)                                
      Histiocytic Sarcoma                              2 (3%)                    2 (3%)       2 (3%)       3 (4%)       1 (1%)      
      Leiomyosarcoma, Metastatic, Intestine Small                                             1 (1%)                                
      Leiomyosarcoma, Metastatic, Stomach                                                                  1 (1%)                   
      Luteoma                                                                                 1 (1%)                                
      Mixed Tumor Benign                                                                      2 (3%)       6 (9%)       1 (1%)      
      Thecoma Benign                                   1 (1%)                                                                       
      Bilateral, Granulosa Cell Tumor Benign                                                               1 (1%)                   
   Uterus                                             (70)         (50)         (60)         (69)         (70)         (88)         
      Adenocarcinoma                                                1 (2%)                    2 (3%)       1 (1%)                   
      Hemangioma                                       1 (1%)       1 (2%)                                 1 (1%)                   
      Histiocytic Sarcoma                                                        2 (3%)       1 (1%)       1 (1%)       1 (1%)      
      Leiomyoma                                                                               1 (1%)                                
      Leiomyosarcoma                                                1 (2%)       1 (2%)                                             
      Polyp Stromal                                    4 (6%)       2 (4%)                    3 (4%)       1 (1%)       4 (5%)      
      Sarcoma                                                       1 (2%)                                                          
      Sarcoma Stromal                                                                         1 (1%)                                
      Cervix, Hemangiosarcoma                                                                 1 (1%)                                
   Vagina                                                                                    (3)                                    
      Hemangiosarcoma                                                                         1 (33%)                               
      Leiomyosarcoma                                                                          1 (33%)                               
      Squamous Cell Carcinoma                                                                 1 (33%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (70)         (49)         (48)         (59)         (70)         (89)         
      Hemangioma                                                    1 (2%)                                                          
      Hemangiosarcoma, Metastatic, Skin                                                       1 (2%)                                
      Hemangiosarcoma, Metastatic, Spleen              1 (1%)                                                                       
      Histiocytic Sarcoma                              1 (1%)                    1 (2%)       1 (2%)       4 (6%)       2 (2%)      
   Lymph Node                                         (70)         (50)         (50)         (60)         (69)         (89)         
      Histiocytic Sarcoma                                                                                               1 (1%)      
      Axillary, Sarcoma, Metastatic, Skin                                                                  1 (1%)                   
      Iliac, Histiocytic Sarcoma                                                 1 (2%)                                             
      Pancreatic, Histiocytic Sarcoma                                                                      1 (1%)                   
      Pancreatic, Leiomyosarcoma, Metastatic,                                                                                       
          Stomach                                                                                          1 (1%)                   
      Renal, Histiocytic Sarcoma                                    1 (2%)       2 (4%)                                             
   Lymph Node, Bronchial                              (58)         (42)         (42)         (41)         (46)         (62)         
                                                             Page   5                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        CONTROL      6.25 PPM     20 PPM       62.5 PPM     200 PPM      625 PPM         
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
HEMATOPOIETIC SYSTEM - cont                                                                                                         
      Adenocarcinoma, Metastatic, Lung                              1 (2%)                                 1 (2%)                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                                            1 (2%)       1 (2%)      
      Carcinoma, Metastatic, Ovary                                                            1 (2%)                                
      Hemangiosarcoma, Metastatic, Heart                                                      1 (2%)                                
      Histiocytic Sarcoma                              2 (3%)                    3 (7%)       1 (2%)       3 (7%)       1 (2%)      
      Sarcoma, Metastatic, Skin                                                               1 (2%)                                
   Lymph Node, Mandibular                             (53)         (42)         (46)         (49)         (54)         (64)         
      Adenocarcinoma, Metastatic, Mammary Gland                                                                         1 (2%)      
      Histiocytic Sarcoma                              2 (4%)       1 (2%)       3 (7%)                    2 (4%)                   
      Sarcoma, Metastatic, Skin                                                  1 (2%)                                             
   Lymph Node, Mediastinal                            (39)         (29)         (33)         (44)         (51)         (74)         
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                               1 (2%)       2 (4%)       2 (3%)      
      Carcinoma, Metastatic, Ovary                                                            1 (2%)                                
      Hepatocellular Carcinoma, Metastatic, Liver                                1 (3%)                                             
      Histiocytic Sarcoma                              2 (5%)       1 (3%)       1 (3%)       1 (2%)       3 (6%)       1 (1%)      
      Sarcoma, Metastatic, Mammary Gland                                                                                1 (1%)      
      Squamous Cell Carcinoma, Metastatic, Stomach                                                                      2 (3%)      
   Lymph Node, Mesenteric                             (69)         (46)         (48)         (58)         (66)         (81)         
      Carcinoma, Metastatic, Ovary                                                            1 (2%)                                
      Histiocytic Sarcoma                              2 (3%)       2 (4%)       5 (10%)      3 (5%)       6 (9%)       4 (5%)      
      Leiomyosarcoma, Metastatic, Intestine Small                                             1 (2%)                                
      Leiomyosarcoma, Metastatic, Stomach                                                                  1 (2%)                   
      Squamous Cell Carcinoma, Metastatic, Stomach                                                                      1 (1%)      
   Spleen                                             (69)         (49)         (48)         (58)         (70)         (88)         
      Hemangioma                                                    1 (2%)                                                          
      Hemangiosarcoma                                  2 (3%)       1 (2%)                                              1 (1%)      
      Histiocytic Sarcoma                              2 (3%)       2 (4%)       4 (8%)       3 (5%)       4 (6%)       4 (5%)      
   Thymus                                             (66)         (46)         (45)         (51)         (59)         (81)         
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                                                         1 (1%)      
      Histiocytic Sarcoma                              2 (3%)                                                           3 (4%)      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (69)         (46)         (49)         (61)         (58)         (87)         
      Adenoacanthoma                                                1 (2%)       2 (4%)       6 (10%)      4 (7%)                   
      Adenocarcinoma                                                2 (4%)       2 (4%)       6 (10%)     12 (21%)     13 (15%)     
      Adenocarcinoma, Multiple                                                                             1 (2%)                   
      Histiocytic Sarcoma                                                                                               1 (1%)      
      Mixed Tumor Malignant                                                                                             4 (5%)      
      Sarcoma                                                                                                           1 (1%)      
   Skin                                               (70)         (50)         (50)         (53)         (60)         (89)         
      Keratoacanthoma                                                                                                   1 (1%)      
      Leiomyosarcoma, Metastatic, Stomach                                                                  1 (2%)                   
                                                             Page   6                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        CONTROL      6.25 PPM     20 PPM       62.5 PPM     200 PPM      625 PPM         
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
INTEGUMENTARY SYSTEM - cont                                                                                                         
      Sebaceous Gland, Adenoma                                                                             1 (2%)                   
      Subcutaneous Tissue, Hemangioma                                            1 (2%)       1 (2%)                                
      Subcutaneous Tissue, Hemangiosarcoma             1 (1%)       2 (4%)       2 (4%)       1 (2%)       2 (3%)       2 (2%)      
      Subcutaneous Tissue, Hemangiosarcoma,                                                                                         
          Multiple                                                                            1 (2%)                                
      Subcutaneous Tissue, Hemangiosarcoma,                                                                                         
          Metastatic, Heart                                                                                             2 (2%)      
      Subcutaneous Tissue, Neurofibrosarcoma                                     1 (2%)                                 1 (1%)      
      Subcutaneous Tissue, Sarcoma                     1 (1%)       2 (4%)       2 (4%)       5 (9%)       3 (5%)       2 (2%)      
      Subcutaneous Tissue, Sarcoma, Multiple                                                                            1 (1%)      
      Subcutaneous Tissue, Squamous Cell Carcinoma,                                                                                 
           Metastatic, Stomach                                                                                          1 (1%)      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (70)         (50)         (50)         (50)         (60)         (90)         
      Adenocarcinoma, Metastatic, Harderian Gland                   1 (2%)                                                          
      Hemangiosarcoma, Metastatic, Skin                                                       1 (2%)                                
   Skeletal Muscle                                    (1)                       (1)          (1)          (2)          (2)          
      Adenocarcinoma, Metastatic, Lung                                           1 (100%)                                           
      Hemangiosarcoma, Metastatic, Spleen              1 (100%)                                                                     
      Hepatocellular Carcinoma, Metastatic, Liver                                1 (100%)                                           
      Diaphragm, Leiomyosarcoma, Metastatic,                                                                                        
          Intestine Small                                                                     1 (100%)                              
      Diaphragm, Sarcoma, Metastatic, Skin                                                                 1 (50%)                  
      Diaphragm, Sarcoma, Metastatic, Stomach                                                                           1 (50%)     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (70)         (50)         (50)         (50)         (60)         (90)         
      Hemangiosarcoma, Metastatic, Heart                                                                   2 (3%)       1 (1%)      
      Histiocytic Sarcoma                                           1 (2%)       2 (4%)       2 (4%)       4 (7%)       1 (1%)      
   Spinal Cord                                                                                            (1)          (9)          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (70)         (60)         (60)         (70)         (70)         (88)         
      Adenocarcinoma                                                1 (2%)       2 (3%)       2 (3%)                                
      Adenocarcinoma, Multiple                                      1 (2%)                                 1 (1%)                   
      Adenocarcinoma, Metastatic, Harderian Gland                   1 (2%)       1 (2%)                    1 (1%)                   
      Adenocarcinoma, Metastatic, Mammary Gland                     1 (2%)                    1 (1%)       3 (4%)       5 (6%)      
      Adenocarcinoma, Metastatic, Uterus                            1 (2%)                                                          
      Alveolar/Bronchiolar Adenoma                     4 (6%)      11 (18%)      9 (15%)     16 (23%)     13 (19%)     15 (17%)     
      Alveolar/Bronchiolar Adenoma, Multiple                                     3 (5%)       4 (6%)       5 (7%)       4 (5%)      
                                                             Page   7                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        CONTROL      6.25 PPM     20 PPM       62.5 PPM     200 PPM      625 PPM         
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
RESPIRATORY SYSTEM - cont                                                                                                           
      Alveolar/Bronchiolar Carcinoma                                3 (5%)      10 (17%)      5 (7%)       9 (13%)      5 (6%)      
      Alveolar/Bronchiolar Carcinoma, Multiple                                                2 (3%)      10 (14%)      3 (3%)      
      Carcinoma, Metastatic, Ovary                                                            1 (1%)                                
      Carcinoma, Metastatic, Parathyroid Gland                                   1 (2%)                                             
      Granulosa Cell Tumor Malignant, Metastatic,                                                                                   
           Ovary                                                                              1 (1%)       1 (1%)                   
      Hemangiosarcoma, Metastatic, Heart                                                                   6 (9%)      15 (17%)     
      Hemangiosarcoma, Metastatic, Skin                                                                                 1 (1%)      
      Hepatocellular Carcinoma, Metastatic, Liver                   1 (2%)       5 (8%)       2 (3%)       3 (4%)       1 (1%)      
      Histiocytic Sarcoma                              2 (3%)       2 (3%)       5 (8%)       3 (4%)       7 (10%)      4 (5%)      
      Mixed Tumor Malignant, Metastatic, Mammary                                                                                    
          Gland                                                                                                         3 (3%)      
      Pheochromocytoma Malignant, Metastatic,                                                                                       
          Adrenal Gland                                                          1 (2%)                                             
      Sarcoma, Metastatic, Skin                                                               1 (1%)       1 (1%)       1 (1%)      
      Squamous Cell Carcinoma, Metastatic, Stomach                                                                      1 (1%)      
      Fat, Mediastinum, Hemangioma                                               1 (2%)                                             
      Mediastinum, Adenocarcinoma, Metastatic, Lung                 1 (2%)       2 (3%)       1 (1%)                                
      Mediastinum, Alveolar/Bronchiolar Carcinoma,                                                                                  
           Metastatic, Lung                                                                   1 (1%)       2 (3%)       3 (3%)      
      Mediastinum, Hemangiosarcoma, Metastatic,                                                                                     
           Heart                                                                              1 (1%)       2 (3%)       1 (1%)      
      Mediastinum, Hemangiosarcoma, Metastatic,                                                                                     
           Skin                                                                  1 (2%)                                             
      Mediastinum, Hepatocellular Carcinoma,                                                                                        
          Metastatic, Liver                                                      1 (2%)                                             
      Mediastinum, Leiomyosarcoma, Metastatic,                                                                                      
           Uterus                                                                1 (2%)                                             
      Mediastinum, Mixed Tumor Malignant,                                                                                           
          Metastatic, Mammary Gland                                                                                     1 (1%)      
      Mediastinum, Sarcoma, Metastatic, Skin           1 (1%)                                                           1 (1%)      
      Mediastinum, Squamous Cell Carcinoma,                                                                                         
          Metastatic, Stomach                                                                                           1 (1%)      
   Nose                                               (70)         (49)         (48)         (50)         (60)         (89)         
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Harderian Gland                                    (64)         (50)         (49)         (59)         (60)         (73)         
      Adenocarcinoma                                                1 (2%)       1 (2%)                    1 (2%)                   
      Adenoma                                         10 (16%)     10 (20%)      7 (14%)     14 (24%)     21 (35%)     10 (14%)     
      Bilateral, Adenoma                                            1 (2%)                    2 (3%)       2 (3%)                   
   Zymbal's Gland                                                                                                      (2)          
      Adenoma                                                                                                           1 (50%)     
      Carcinoma                                                                                                         1 (50%)     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                             Page   8                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        CONTROL      6.25 PPM     20 PPM       62.5 PPM     200 PPM      625 PPM         
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (69)         (49)         (48)         (50)         (60)         (90)         
      Adenocarcinoma, Metastatic, Lung                              1 (2%)       1 (2%)                                             
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                  1 (2%)                                 1 (1%)      
      Carcinoma, Metastatic, Parathyroid Gland                                   1 (2%)                                             
      Hemangiosarcoma, Metastatic, Heart                                                                   1 (2%)       1 (1%)      
      Histiocytic Sarcoma                              2 (3%)       2 (4%)       5 (10%)      2 (4%)       5 (8%)       4 (4%)      
      Leiomyosarcoma, Metastatic, Stomach                                                                  1 (2%)                   
      Squamous Cell Carcinoma, Metastatic, Stomach                                                                      1 (1%)      
      Renal Tubule, Adenoma                                                                                2 (3%)                   
   Urinary Bladder                                    (67)         (46)         (48)         (46)         (59)         (85)         
      Histiocytic Sarcoma                                           1 (2%)                                 1 (2%)                   
      Squamous Cell Carcinoma, Metastatic, Stomach                                                                      1 (1%)      
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SYSTEMIC LESIONS                                                                                                                   
   Multiple Organs                                   *(70)        *(70)        *(70)        *(70)        *(70)        *(90)         
      Histiocytic Sarcoma                              3 (4%)       2 (3%)       7 (10%)      4 (6%)       7 (10%)      4 (4%)      
      Leukemia Granulocytic                                                                                1 (1%)                   
      Lymphoma Malignant                                                                      1 (1%)                    1 (1%)      
      Lymphoma Malignant Lymphocytic                   2 (3%)       3 (4%)       6 (9%)       3 (4%)       9 (13%)     32 (36%)     
      Lymphoma Malignant Mixed                         5 (7%)       9 (13%)      4 (6%)       2 (3%)       1 (1%)                   
      Lymphoma Malignant Undifferentiated Cell                                   1 (1%)       1 (1%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
     *   Number of animals with any tissue examined microscopically                                                                 
                                                             Page   9                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        CONTROL      6.25 PPM     20 PPM       62.5 PPM     200 PPM      625 PPM         
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
TUMOR SUMMARY                                                                                                                       
                                                                                                                                    
   Total Animals with Primary Neoplasms (b)            40          49          44          54          61          77               
     Total Primary Neoplasms                           65          97          93         146         191         169               
                                                                                                                                    
   Total Animals with Benign Neoplasms                 33          38          25          44          47          41               
     Total Benign Neoplasms                            46          57          41          81          92          63               
                                                                                                                                    
   Total Animals with Malignant Neoplasms              16          32          40          42          56          74               
     Total Malignant Neoplasms                         19          40          52          65          99         106               
                                                                                                                                    
   Total Animals with Metastatic Neoplasms              2           5          12          12          22          33               
     Total Metastatic Neoplasm                          5          11          23          27          54          94               
                                                                                                                                    
   Total Animals with Malignant Neoplasms                                                                                           
   Uncertain Primary Site                                                                                                           
                                                                                                                                    
   Total Animals with Neoplasms Uncertain-                                                                                          
   Benign or Malignant                                                                                                              
     Total Uncertain Neoplasms                                                                                                      
                                                                                                                                    
____________________________________________________________________________________________________________________________________
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Primary tumors: all tumors except metastatic tumors                                                                              
                                                             Page  10                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          CONTROL      6.25 PPM     20 PPM       62.5 PPM     200 PPM      200 PPM         
                                                                                                                    SE40            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
  Animals Initially in Study                          70           70           70           70           70           50           
  Scheduled Sacrifice                                 20           20           20           20           20                        
  Early Deaths                                                                                                                      
    Dead                                               6            5           11           12           23           12           
    Moribund                                           9            6           15           15           23           29           
  Survivors                                                                                                                         
    Terminal Sacrifice                                35           39           24           22            3            9           
    Dead                                                                                                   1                        
  Missing                                                                                     1                                     
  Animals Examined Microscopically                    70           70           70           70           70           50           
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Gallbladder                                        (64)         (47)         (39)         (36)         (42)         (40)         
   Intestine Large, Colon                             (70)         (47)         (45)         (46)         (55)         (44)         
      Mast Cell Tumor NOS, Metastatic, Bone Marrow                                                                      1 (2%)      
   Intestine Small, Duodenum                          (67)         (46)         (42)         (44)         (47)         (42)         
      Histiocytic Sarcoma                                                                     1 (2%)                                
   Intestine Small, Ileum                             (68)         (48)         (43)         (46)         (47)         (44)         
      Histiocytic Sarcoma                                                                                               1 (2%)      
   Intestine Small, Jejunum                           (68)         (46)         (43)         (44)         (43)         (43)         
      Carcinoma                                                     1 (2%)       1 (2%)       1 (2%)       2 (5%)                   
   Liver                                              (70)         (61)         (61)         (60)         (68)         (49)         
      Adenocarcinoma, Metastatic, Lung                                                                     1 (1%)                   
      Hemangioma                                                                              1 (2%)                    1 (2%)      
      Hemangiosarcoma                                               2 (3%)                                                          
      Hemangiosarcoma, Metastatic, Heart                                                      3 (5%)       9 (13%)      4 (8%)      
      Hepatocellular Carcinoma                         8 (11%)     13 (21%)     10 (16%)     11 (18%)     18 (26%)      9 (18%)     
      Hepatocellular Carcinoma, Multiple               3 (4%)       4 (7%)       6 (10%)      6 (10%)      9 (13%)      5 (10%)     
      Hepatocellular Adenoma                          17 (24%)     12 (20%)     16 (26%)     14 (23%)     22 (32%)     15 (31%)     
      Hepatocellular Adenoma, Multiple                 2 (3%)       1 (2%)       8 (13%)      5 (8%)       7 (10%)     12 (24%)     
      Hepatocholangiocarcinoma                         1 (1%)                                              1 (1%)                   
      Histiocytic Sarcoma                                                        4 (7%)       5 (8%)       7 (10%)      4 (8%)      
      Mast Cell Tumor NOS, Metastatic, Bone Marrow                                                         1 (1%)       2 (4%)      
      Sarcoma, Metastatic, Skin                        1 (1%)                                                                       
   Mesentery                                          (6)          (5)          (9)          (14)         (10)         (13)         
      Fat, Adenocarcinoma, Metastatic, Lung                                                                1 (10%)                  
      Fat, Hemangioma                                                                         2 (14%)                   2 (15%)     
      Fat, Hemangioma, Multiple                                                                                         1 (8%)      
      Fat, Hemangiosarcoma                                                                                              1 (8%)      
      Fat, Hemangiosarcoma, Metastatic, Heart                                                 1 (7%)                    2 (15%)     
      Fat, Hemangiosarcoma, Metastatic, Spleen                                                             1 (10%)                  
                                                             Page  11                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          CONTROL      6.25 PPM     20 PPM       62.5 PPM     200 PPM      200 PPM         
                                                                                                                    SE40            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - cont                                                                                                            
      Fat, Histiocytic Sarcoma                                                                1 (7%)       3 (30%)                  
      Fat, Mast Cell Tumor NOS, Metastatic, Bone                                                                                    
          Marrow                                                                                                        2 (15%)     
      Fat, Sarcoma, Metastatic, Skin                   1 (17%)                                                                      
   Pancreas                                           (70)         (49)         (49)         (58)         (66)         (46)         
      Acinus, Hemangiosarcoma, Metastatic, Heart                                 1 (2%)                                             
      Acinus, Histiocytic Sarcoma                                                                          1 (2%)       2 (4%)      
      Acinus, Sarcoma, Metastatic, Skin                1 (1%)                                                                       
      Duct, Adenoma, Multiple                                                                              1 (2%)                   
   Pharynx                                                                                   (1)          (1)          (1)          
      Palate, Mast Cell Tumor NOS, Metastatic,                                                                                      
           Bone Marrow                                                                                                  1 (100%)    
   Salivary Glands                                    (70)         (50)         (50)         (48)         (58)         (48)         
      Histiocytic Sarcoma                                                                     1 (2%)                                
   Stomach, Forestomach                               (70)         (50)         (60)         (60)         (68)         (48)         
      Mast Cell Tumor NOS, Metastatic, Bone Marrow     1 (1%)                                              1 (1%)       3 (6%)      
      Sarcoma, Metastatic, Skin                        1 (1%)                                                                       
      Squamous Cell Carcinoma                                                                              2 (3%)                   
      Squamous Cell Papilloma                          1 (1%)                                 1 (2%)       7 (10%)      2 (4%)      
      Squamous Cell Papilloma, Multiple                                                                                 1 (2%)      
   Stomach, Glandular                                 (70)         (48)         (60)         (60)         (65)         (44)         
      Mast Cell Tumor NOS, Metastatic, Bone Marrow                                                                      1 (2%)      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (70)         (50)         (60)         (58)         (68)         (50)         
      Adenocarcinoma, Metastatic, Lung                                                        2 (3%)       2 (3%)                   
      Hemangiosarcoma                                                            1 (2%)       5 (9%)      21 (31%)     15 (30%)     
      Hepatocellular Carcinoma, Metastatic, Liver                                1 (2%)                                             
      Histiocytic Sarcoma                                                        1 (2%)       1 (2%)       3 (4%)                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Gland, Cortex                              (70)         (49)         (50)         (47)         (58)         (45)         
      Adenoma                                          1 (1%)       4 (8%)                    1 (2%)                    1 (2%)      
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                                                         1 (2%)      
      Histiocytic Sarcoma                                                                     2 (4%)       1 (2%)                   
      Bilateral, Adenoma                               1 (1%)                                                                       
   Adrenal Gland, Medulla                             (70)         (48)         (47)         (44)         (54)         (45)         
      Pheochromocytoma Benign                                       1 (2%)                                                          
   Islets, Pancreatic                                 (70)         (49)         (49)         (58)         (65)         (46)         
      Adenoma                                                                    1 (2%)                                 1 (2%)      
   Pituitary Gland                                    (69)         (47)         (47)         (48)         (56)         (44)         
      Pars Distalis, Adenoma                           1 (1%)                                                                       
                                                             Page  12                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          CONTROL      6.25 PPM     20 PPM       62.5 PPM     200 PPM      200 PPM         
                                                                                                                    SE40            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ENDOCRINE SYSTEM - cont                                                                                                             
      Pars Distalis, Carcinoma                                                                1 (2%)                                
      Pars Intermedia, Adenoma                                                                             1 (2%)       1 (2%)      
   Thyroid Gland                                      (70)         (50)         (50)         (48)         (57)         (48)         
      Mast Cell Tumor NOS, Metastatic, Bone Marrow                                                                      1 (2%)      
      Follicular Cell, Adenoma                         2 (3%)       4 (8%)                    4 (8%)       2 (4%)       2 (4%)      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Epididymis                                         (70)         (50)         (50)         (48)         (59)         (49)         
      Hemangiosarcoma, Metastatic, Heart                                                                                1 (2%)      
   Preputial Gland                                    (8)          (2)          (4)          (9)          (6)          (8)          
      Carcinoma                                                                                            5 (83%)                  
      Hemangiosarcoma                                                                         1 (11%)                               
      Bilateral, Carcinoma                                                                                              1 (13%)     
   Prostate                                           (67)         (50)         (48)         (45)         (56)         (44)         
   Seminal Vesicle                                    (70)         (48)         (47)         (46)         (54)         (44)         
      Hemangioma                                                    1 (2%)                                                          
      Hemangiosarcoma, Metastatic, Spleen                           1 (2%)                                                          
   Testes                                             (70)         (50)         (51)         (48)         (69)         (50)         
      Hemangioma                                                                              1 (2%)                                
      Interstitial Cell, Adenoma                       1 (1%)                                 1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (70)         (50)         (50)         (58)         (69)         (50)         
      Histiocytic Sarcoma                                                        1 (2%)       2 (3%)       4 (6%)       3 (6%)      
      Mast Cell Tumor NOS                              1 (1%)                                              1 (1%)       3 (6%)      
   Lymph Node                                         (70)         (50)         (50)         (57)         (68)         (50)         
      Hemangioma                                       1 (1%)                                                                       
      Mast Cell Tumor NOS, Metastatic, Bone Marrow     1 (1%)                                                           1 (2%)      
      Iliac, Histiocytic Sarcoma                                                 1 (2%)       1 (2%)                                
      Pancreatic, Histiocytic Sarcoma                                                         1 (2%)                                
   Lymph Node, Bronchial                              (64)         (42)         (42)         (40)         (46)         (43)         
      Adenocarcinoma, Metastatic, Lung                                           1 (2%)       2 (5%)       2 (4%)                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                  1 (2%)                                             
      Hepatocholangiocarcinoma, Metastatic, Liver      1 (2%)                                                                       
      Histiocytic Sarcoma                                                        1 (2%)       2 (5%)       3 (7%)       2 (5%)      
   Lymph Node, Mandibular                             (55)         (39)         (38)         (37)         (53)         (42)         
      Histiocytic Sarcoma                                                        1 (3%)       1 (3%)       2 (4%)       2 (5%)      
                                                             Page  13                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          CONTROL      6.25 PPM     20 PPM       62.5 PPM     200 PPM      200 PPM         
                                                                                                                    SE40            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
HEMATOPOIETIC SYSTEM - cont                                                                                                         
      Mast Cell Tumor NOS, Metastatic, Bone Marrow     1 (2%)                                                           2 (5%)      
      Sarcoma, Metastatic, Skin                                                  1 (3%)                                             
   Lymph Node, Mediastinal                            (37)         (27)         (33)         (39)         (48)         (39)         
      Adenocarcinoma, Metastatic, Lung                                                        2 (5%)       1 (2%)                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                                            1 (2%)                   
      Hemangiosarcoma, Metastatic, Heart                                                                                1 (3%)      
      Hepatocholangiocarcinoma, Metastatic, Liver      1 (3%)                                                                       
      Histiocytic Sarcoma                                                        2 (6%)       1 (3%)       4 (8%)       1 (3%)      
      Mast Cell Tumor NOS, Metastatic, Bone Marrow                                                                      2 (5%)      
   Lymph Node, Mesenteric                             (68)         (47)         (47)         (55)         (65)         (44)         
      Hemangiosarcoma, Metastatic, Heart                                                                                1 (2%)      
      Histiocytic Sarcoma                                                        3 (6%)       4 (7%)       6 (9%)       4 (9%)      
      Mast Cell Tumor NOS, Metastatic, Bone Marrow                                                                      1 (2%)      
   Spleen                                             (70)         (50)         (51)         (58)         (66)         (47)         
      Hemangiosarcoma                                               1 (2%)       1 (2%)                    1 (2%)                   
      Hemangiosarcoma, Metastatic, Heart                                                                                1 (2%)      
      Histiocytic Sarcoma                                                        4 (8%)       4 (7%)       6 (9%)       4 (9%)      
      Mast Cell Tumor NOS, Metastatic, Bone Marrow                                                         1 (2%)       2 (4%)      
   Thymus                                             (66)         (43)         (42)         (51)         (55)         (41)         
      Adenocarcinoma, Metastatic, Lung                                                                     1 (2%)                   
      Histiocytic Sarcoma                                                        1 (2%)                    2 (4%)       1 (2%)      
      Mast Cell Tumor NOS, Metastatic, Bone Marrow                                                         1 (2%)       2 (5%)      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Skin                                               (70)         (50)         (51)         (49)         (60)         (50)         
      Mast Cell Tumor NOS, Metastatic, Bone Marrow                                                                      1 (2%)      
      Sebaceous Gland, Adenoma                                                                1 (2%)       1 (2%)                   
      Subcutaneous Tissue, Adenocarcinoma,                                                                                          
          Metastatic, Lung                                                                                 1 (2%)                   
      Subcutaneous Tissue, Hemangioma                                                                                   1 (2%)      
      Subcutaneous Tissue, Hemangiosarcoma                          1 (2%)       1 (2%)                    3 (5%)       1 (2%)      
      Subcutaneous Tissue, Hemangiosarcoma,                                                                                         
          Metastatic, Heart                                                                                             2 (4%)      
      Subcutaneous Tissue, Leiomyosarcoma                                                     1 (2%)                                
      Subcutaneous Tissue, Mast Cell Tumor NOS                                   1 (2%)                                             
      Subcutaneous Tissue, Mixed Tumor Malignant                                              1 (2%)                                
      Subcutaneous Tissue, Sarcoma                     1 (1%)                                                                       
      Subcutaneous Tissue, Head, Sarcoma                                         1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                             Page  14                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          CONTROL      6.25 PPM     20 PPM       62.5 PPM     200 PPM      200 PPM         
                                                                                                                    SE40            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Skeletal Muscle                                    (2)                                    (2)          (2)                       
      Adenocarcinoma, Metastatic, Lung                                                                     2 (100%)                 
      Hemangiosarcoma                                  1 (50%)                                1 (50%)                               
      Diaphragm, Adenocarcinoma, Metastatic, Lung                                             1 (50%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (70)         (50)         (50)         (48)         (60)         (50)         
      Carcinoma, Metastatic, Pituitary Gland                                                  1 (2%)                                
      Glioma Malignant                                                                                     1 (2%)                   
      Hemangiosarcoma, Metastatic, Heart                                         1 (2%)                                             
      Histiocytic Sarcoma                                                        1 (2%)       1 (2%)       5 (8%)       2 (4%)      
      Mast Cell Tumor NOS, Metastatic, Bone Marrow                                                                      1 (2%)      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (70)         (52)         (62)         (69)         (70)         (50)         
      Adenocarcinoma                                                             1 (2%)       2 (3%)       4 (6%)                   
      Adenocarcinoma, Multiple                                                   1 (2%)                                             
      Adenocarcinoma, Metastatic, Harderian Gland                   1 (2%)       1 (2%)       2 (3%)       2 (3%)       2 (4%)      
      Alveolar/Bronchiolar Adenoma                    14 (20%)     15 (29%)     11 (18%)     20 (29%)     18 (26%)     16 (32%)     
      Alveolar/Bronchiolar Adenoma, Multiple           4 (6%)       6 (12%)                   6 (9%)       8 (11%)      8 (16%)     
      Alveolar/Bronchiolar Carcinoma                   4 (6%)       6 (12%)      7 (11%)      9 (13%)     12 (17%)     13 (26%)     
      Alveolar/Bronchiolar Carcinoma, Multiple         2 (3%)                    2 (3%)       2 (3%)      12 (17%)      9 (18%)     
      Carcinoma, Metastatic, Liver                     1 (1%)                                                                       
      Hemangiosarcoma, Metastatic, Heart                                                      3 (4%)       8 (11%)      3 (6%)      
      Hemangiosarcoma, Metastatic, Skeletal Muscle                                            1 (1%)                                
      Hepatocellular Carcinoma, Metastatic                                                                 1 (1%)                   
      Hepatocellular Carcinoma, Metastatic, Liver      3 (4%)       3 (6%)       4 (6%)       3 (4%)       4 (6%)       2 (4%)      
      Hepatocholangiocarcinoma, Metastatic, Liver      1 (1%)                                                                       
      Histiocytic Sarcoma                                                        4 (6%)       4 (6%)       7 (10%)      4 (8%)      
      Leiomyosarcoma, Metastatic, Skin                                                        1 (1%)                                
      Mast Cell Tumor NOS, Metastatic, Bone Marrow                                                                      1 (2%)      
      Sarcoma, Metastatic, Skin                        1 (1%)                    1 (2%)                                             
      Mediastinum, Adenocarcinoma, Metastatic, Lung                                           2 (3%)       3 (4%)                   
      Mediastinum, Alveolar/Bronchiolar Carcinoma,                                                                                  
           Metastatic, Lung                                                                                1 (1%)                   
      Mediastinum, Hemangiosarcoma, Metastatic,                                                                                     
           Heart                                                                                           3 (4%)                   
      Mediastinum, Hepatocholangiocarcinoma,                                                                                        
          Metastatic, Liver                            1 (1%)                                                                       
      Mediastinum, Sarcoma, Metastatic, Skin                                     1 (2%)                                             
   Nose                                               (70)         (50)         (50)         (47)         (59)         (49)         
      Histiocytic Sarcoma                                                                                  1 (2%)                   
                                                             Page  15                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          CONTROL      6.25 PPM     20 PPM       62.5 PPM     200 PPM      200 PPM         
                                                                                                                    SE40            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
RESPIRATORY SYSTEM - cont                                                                                                           
      Leiomyosarcoma, Metastatic, Skin                                                        1 (2%)                                
      Mast Cell Tumor NOS, Metastatic, Bone Marrow                                                                      1 (2%)      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Harderian Gland                                    (63)         (59)         (60)         (57)         (61)         (48)         
      Adenocarcinoma                                                1 (2%)       1 (2%)       3 (5%)       2 (3%)       2 (4%)      
      Adenoma                                          6 (10%)      7 (12%)     10 (17%)     21 (37%)     26 (43%)     22 (46%)     
      Leiomyosarcoma, Metastatic, Skin                                                        1 (2%)                                
      Bilateral, Adenoma                                                                      2 (4%)       7 (11%)      4 (8%)      
   Zymbal's Gland                                     (2)                                                              (1)          
      Adenoma                                          1 (50%)                                                                      
      Carcinoma                                                                                                         1 (100%)    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (70)         (53)         (54)         (50)         (69)         (48)         
      Adenocarcinoma, Metastatic, Harderian Gland                                                          1 (1%)                   
      Adenocarcinoma, Metastatic, Lung                                                        2 (4%)       1 (1%)                   
      Hemangiosarcoma, Metastatic, Heart                                         1 (2%)       1 (2%)       2 (3%)       1 (2%)      
      Hemangiosarcoma, Metastatic, Mesentery                                                                            1 (2%)      
      Histiocytic Sarcoma                                                        1 (2%)       3 (6%)       6 (9%)       2 (4%)      
      Mast Cell Tumor NOS, Metastatic, Bone Marrow                                                         1 (1%)       1 (2%)      
      Renal Tubule, Adenocarcinoma                                                            1 (2%)                                
      Renal Tubule, Adenoma                                         1 (2%)                    2 (4%)       1 (1%)       4 (8%)      
   Urinary Bladder                                    (70)         (48)         (50)         (49)         (58)         (43)         
      Histiocytic Sarcoma                                                                                  1 (2%)                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SYSTEMIC LESIONS                                                                                                                   
   Multiple Organs                                   *(70)        *(70)        *(70)        *(70)        *(70)        *(50)         
      Histiocytic Sarcoma                                                        4 (6%)       5 (7%)       7 (10%)      5 (10%)     
      Lymphoma Malignant                                                                      1 (1%)                    1 (2%)      
      Lymphoma Malignant Histiocytic                                                          1 (1%)                                
      Lymphoma Malignant Lymphocytic                   2 (3%)                    2 (3%)       4 (6%)       2 (3%)       6 (12%)     
      Lymphoma Malignant Mixed                         2 (3%)       1 (1%)       2 (3%)                                 1 (2%)      
      Lymphoma Malignant Undifferentiated Cell                      1 (1%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
     *   Number of animals with any tissue examined microscopically                                                                 
                                                             Page  16                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          CONTROL      6.25 PPM     20 PPM       62.5 PPM     200 PPM      200 PPM         
                                                                                                                    SE40            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
TUMOR SUMMARY                                                                                                                       
                                                                                                                                    
   Total Animals with Primary Neoplasms (b)            51          42          52          54          58          49               
     Total Primary Neoplasms                           77          83          88         138         204         167               
                                                                                                                                    
   Total Animals with Benign Neoplasms                 42          36          41          45          52          41               
     Total Benign Neoplasms                            52          52          46          82         101          94               
                                                                                                                                    
   Total Animals with Malignant Neoplasms              20          27          34          43          50          43               
     Total Malignant Neoplasms                         24          31          41          56         102          70               
                                                                                                                                    
   Total Animals with Metastatic Neoplasms              6           5           9          13          20          14               
     Total Metastatic Neoplasm                         16           5          14          29          53          48               
                                                                                                                                    
   Total Animals with Malignant Neoplasms                                                                                           
   Uncertain Primary Site                                                                                                           
                                                                                                                                    
   Total Animals with Neoplasms Uncertain-                                                                                          
   Benign or Malignant                                  1                       1                       1           3               
     Total Uncertain Neoplasms                          1                       1                       1           3               
                                                                                                                                    
____________________________________________________________________________________________________________________________________
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Primary tumors: all tumors except metastatic tumors                                                                              
                                                             Page  17                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          312 PPM      625 PPM      625 PPM      625 PPM                                   
                                                   SE52                      SE13         SE 26                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
  Animals Initially in Study                          50           90           50           50                                     
  Scheduled Sacrifice                                              17                                                               
  Early Deaths                                                                                                                      
    Moribund                                          25           33           25           22                                     
    Dead                                              24           39           19           28                                     
    Accident                                                        1            1                                                  
  Survivors                                                                                                                         
    Terminal Sacrifice                                 1                         5                                                  
  Animals Examined Microscopically                    50           90           50           50                                     
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Gallbladder                                        (27)         (57)         (32)         (32)                                   
   Intestine Large, Cecum                             (41)         (72)         (39)         (42)                                   
      Histiocytic Sarcoma                                                                     1 (2%)                                
   Intestine Small, Duodenum                          (38)         (67)         (35)         (32)                                   
   Intestine Small, Ileum                             (40)         (68)         (37)         (40)                                   
      Histiocytic Sarcoma                              1 (3%)                                 1 (3%)                                
   Intestine Small, Jejunum                           (37)         (65)         (40)         (41)                                   
      Carcinoma                                                                  1 (3%)                                             
      Hemangiosarcoma, Metastatic, Heart               1 (3%)                                                                       
      Histiocytic Sarcoma                              1 (3%)                    1 (3%)       1 (2%)                                
   Liver                                              (50)         (89)         (49)         (50)                                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                        1 (2%)                                                                       
      Hemangiosarcoma                                  1 (2%)                    2 (4%)       1 (2%)                                
      Hemangiosarcoma, Metastatic, Heart              18 (36%)      3 (3%)       2 (4%)       5 (10%)                               
      Hepatoblastoma                                   1 (2%)                                                                       
      Hepatocellular Carcinoma                         8 (16%)      3 (3%)       7 (14%)      3 (6%)                                
      Hepatocellular Carcinoma, Multiple               2 (4%)       1 (1%)       7 (14%)      1 (2%)                                
      Hepatocellular Adenoma                          10 (20%)      6 (7%)      14 (29%)      7 (14%)                               
      Hepatocellular Adenoma, Multiple                 9 (18%)      3 (3%)       5 (10%)      4 (8%)                                
      Histiocytic Sarcoma                              5 (10%)      4 (4%)       2 (4%)       2 (4%)                                
      Mast Cell Tumor NOS, Metastatic, Bone Marrow     1 (2%)                                 1 (2%)                                
      Squamous Cell Carcinoma, Metastatic, Stomach     3 (6%)       1 (1%)                    1 (2%)                                
   Mesentery                                          (13)         (6)          (11)         (4)                                    
      Adenocarcinoma, Metastatic, Pancreas             1 (8%)                                                                       
      Squamous Cell Carcinoma, Metastatic, Stomach                  1 (17%)                                                         
      Fat, Hemangioma                                                            2 (18%)                                            
      Fat, Hemangiosarcoma                                                       1 (9%)                                             
      Fat, Hemangiosarcoma, Metastatic, Heart          7 (54%)      1 (17%)                                                         
      Fat, Histiocytic Sarcoma                                                                1 (25%)                               
                                                             Page  18                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          312 PPM      625 PPM      625 PPM      625 PPM                                   
                                                   SE52                      SE13         SE 26                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - cont                                                                                                            
      Fat, Squamous Cell Carcinoma, Metastatic,                                                                                     
           Stomach                                     2 (15%)      1 (17%)      2 (18%)      1 (25%)                               
   Pancreas                                           (49)         (89)         (46)         (49)                                   
      Acinus, Adenocarcinoma                           1 (2%)                                                                       
      Acinus, Hemangiosarcoma, Metastatic, Heart       1 (2%)                                                                       
      Acinus, Hemangiosarcoma, Metastatic, Liver                                              1 (2%)                                
      Acinus, Histiocytic Sarcoma                      1 (2%)       2 (2%)       1 (2%)       1 (2%)                                
      Acinus, Squamous Cell Carcinoma, Metastatic,                                                                                  
           Stomach                                     1 (2%)       1 (1%)                    1 (2%)                                
   Pharynx                                                         (2)                       (2)                                    
      Carcinoma, Metastatic, Zymbal's Gland                                                   1 (50%)                               
      Squamous Cell Carcinoma                                                                 1 (50%)                               
      Squamous Cell Papilloma                                       1 (50%)                                                         
   Salivary Glands                                    (48)         (86)         (50)         (50)                                   
      Histiocytic Sarcoma                                           1 (1%)                                                          
   Stomach, Forestomach                               (48)         (89)         (50)         (50)                                   
      Histiocytic Sarcoma                                           1 (1%)                                                          
      Mast Cell Tumor NOS, Metastatic, Bone Marrow                                            1 (2%)                                
      Squamous Cell Carcinoma                          5 (10%)      4 (4%)       4 (8%)       6 (12%)                               
      Squamous Cell Papilloma                          4 (8%)       4 (4%)       3 (6%)       4 (8%)                                
      Squamous Cell Papilloma, Multiple                                          1 (2%)                                             
   Stomach, Glandular                                 (47)         (83)         (44)         (47)                                   
      Histiocytic Sarcoma                                           1 (1%)                    1 (2%)                                
      Squamous Cell Carcinoma, Metastatic, Stomach     1 (2%)       2 (2%)       1 (2%)       1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (50)         (90)         (50)         (50)                                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                  1 (2%)                                             
      Carcinoma, Metastatic, Zymbal's Gland                                                   1 (2%)                                
      Hemangiosarcoma                                 33 (66%)      7 (8%)       7 (14%)     13 (26%)                               
      Histiocytic Sarcoma                              1 (2%)                                 1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Gland, Cortex                              (49)         (90)         (50)         (50)                                   
      Adenocarcinoma, Metastatic, Pancreas             1 (2%)                                                                       
      Bilateral, Adenoma                                                         1 (2%)                                             
   Adrenal Gland, Medulla                             (48)         (86)         (49)         (48)                                   
      Adenocarcinoma, Metastatic, Pancreas             1 (2%)                                                                       
      Histiocytic Sarcoma                                           1 (1%)                    1 (2%)                                
      Pheochromocytoma Benign                          1 (2%)       1 (1%)       2 (4%)                                             
   Islets, Pancreatic                                 (49)         (87)         (45)         (48)                                   
      Adenoma                                          1 (2%)                                                                       
                                                             Page  19                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          312 PPM      625 PPM      625 PPM      625 PPM                                   
                                                   SE52                      SE13         SE 26                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ENDOCRINE SYSTEM - cont                                                                                                             
   Parathyroid Gland                                  (44)         (69)         (33)         (37)                                   
   Pituitary Gland                                    (43)         (78)         (43)         (43)                                   
      Carcinoma, Metastatic, Zymbal's Gland                                                   1 (2%)                                
   Thyroid Gland                                      (49)         (86)         (50)         (49)                                   
      Follicular Cell, Adenoma                         1 (2%)                    2 (4%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Epididymis                                         (50)         (87)         (49)         (50)                                   
      Adenocarcinoma, Metastatic, Pancreas             1 (2%)                                                                       
      Histiocytic Sarcoma                                           1 (1%)                                                          
   Preputial Gland                                    (4)          (2)          (10)         (6)                                    
      Adenoma                                                                    1 (10%)                                            
      Carcinoma                                        3 (75%)                   3 (30%)      2 (33%)                               
      Bilateral, Carcinoma                             1 (25%)                   1 (10%)      1 (17%)                               
   Prostate                                           (48)         (83)         (48)         (47)                                   
   Seminal Vesicle                                    (47)         (84)         (42)         (47)                                   
      Hemangiosarcoma, Metastatic, Liver                                                      1 (2%)                                
      Histiocytic Sarcoma                                           1 (1%)                                                          
   Testes                                             (50)         (89)         (50)         (50)                                   
      Hemangioma                                                                 1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (49)         (90)         (48)         (49)                                   
      Histiocytic Sarcoma                              2 (4%)       4 (4%)       1 (2%)       1 (2%)                                
      Mast Cell Tumor NOS                              1 (2%)                                 1 (2%)                                
   Lymph Node                                         (50)         (90)         (49)         (50)                                   
      Histiocytic Sarcoma                                           1 (1%)                                                          
      Axillary, Hemangiosarcoma, Metastatic, Heart     1 (2%)                                                                       
      Inguinal, Squamous Cell Carcinoma,                                                                                            
          Metastatic, Stomach                                                                 1 (2%)                                
      Renal, Histiocytic Sarcoma                       1 (2%)                    1 (2%)                                             
      Renal, Squamous Cell Carcinoma, Metastatic,                                                                                   
           Stomach                                     1 (2%)                                                                       
   Lymph Node, Bronchial                              (37)         (53)         (40)         (41)                                   
      Adenocarcinoma, Metastatic, Lung                 1 (3%)                                                                       
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                  1 (3%)       1 (2%)                                
      Hemangiosarcoma, Metastatic, Heart               1 (3%)                                 1 (2%)                                
                                                             Page  20                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          312 PPM      625 PPM      625 PPM      625 PPM                                   
                                                   SE52                      SE13         SE 26                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
HEMATOPOIETIC SYSTEM - cont                                                                                                         
      Histiocytic Sarcoma                              3 (8%)                    1 (3%)       1 (2%)                                
      Squamous Cell Carcinoma, Metastatic, Stomach     1 (3%)       1 (2%)                                                          
   Lymph Node, Mandibular                             (31)         (70)         (39)         (43)                                   
      Histiocytic Sarcoma                                           2 (3%)                    2 (5%)                                
      Mast Cell Tumor NOS, Metastatic, Bone Marrow                                            1 (2%)                                
      Squamous Cell Carcinoma, Metastatic, Pharynx                                            1 (2%)                                
   Lymph Node, Mediastinal                            (41)         (68)         (39)         (44)                                   
      Adenocarcinoma, Metastatic, Lung                 1 (2%)                                                                       
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                  1 (3%)       1 (2%)                                
      Histiocytic Sarcoma                              4 (10%)      1 (1%)       1 (3%)       1 (2%)                                
      Squamous Cell Carcinoma, Metastatic, Stomach     2 (5%)       1 (1%)       2 (5%)       2 (5%)                                
   Lymph Node, Mesenteric                             (44)         (84)         (43)         (48)                                   
      Hemangiosarcoma, Metastatic, Heart               1 (2%)                                                                       
      Histiocytic Sarcoma                              6 (14%)      3 (4%)       2 (5%)       1 (2%)                                
      Squamous Cell Carcinoma, Metastatic, Stomach     3 (7%)       1 (1%)       1 (2%)                                             
   Spleen                                             (48)         (87)         (49)         (50)                                   
      Hemangiosarcoma                                                            1 (2%)                                             
      Hemangiosarcoma, Metastatic, Heart               3 (6%)                                                                       
      Histiocytic Sarcoma                              4 (8%)       4 (5%)       2 (4%)       2 (4%)                                
      Mast Cell Tumor NOS, Metastatic, Bone Marrow     1 (2%)                                 1 (2%)                                
      Osteosarcoma, Metastatic, Uncertain Primary                                                                                   
          Site                                                                                1 (2%)                                
      Squamous Cell Carcinoma, Metastatic, Stomach                                            1 (2%)                                
   Thymus                                             (37)         (78)         (37)         (40)                                   
      Histiocytic Sarcoma                              1 (3%)                    1 (3%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Skin                                               (49)         (90)         (50)         (50)                                   
      Sebaceous Gland, Adenoma                         2 (4%)                    2 (4%)                                             
      Subcutaneous Tissue, Hemangiosarcoma                                       1 (2%)                                             
      Subcutaneous Tissue, Hemangiosarcoma,                                                                                         
          Metastatic, Heart                            4 (8%)                                                                       
      Subcutaneous Tissue, Histiocytic Sarcoma                      1 (1%)                                                          
      Subcutaneous Tissue, Leiomyosarcoma                                                     1 (2%)                                
      Subcutaneous Tissue, Squamous Cell Carcinoma,                                                                                 
           Metastatic, Stomach                         1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (50)         (90)         (50)         (50)                                   
      Neuroblastoma Malignant, Metastatic, Brain                                 1 (2%)                                             
   Skeletal Muscle                                                 (1)          (2)          (2)                                    
      Abdominal, Squamous Cell Carcinoma,                                                                                           
                                                             Page  21                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          312 PPM      625 PPM      625 PPM      625 PPM                                   
                                                   SE52                      SE13         SE 26                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
MUSCULOSKELETAL SYSTEM - cont                                                                                                       
          Metastatic, Stomach                                                    1 (50%)                                            
      Diaphragm, Hemangiosarcoma, Metastatic, Liver                                           1 (50%)                               
      Diaphragm, Squamous Cell Carcinoma,                                                                                           
          Metastatic, Stomach                                       1 (100%)                  1 (50%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (50)         (90)         (50)         (50)                                   
      Adenocarcinoma, Metastatic, Harderian Gland                                1 (2%)                                             
      Hemangiosarcoma, Metastatic, Heart               5 (10%)                                                                      
      Histiocytic Sarcoma                              2 (4%)       2 (2%)       1 (2%)       1 (2%)                                
      Olfactory Lobe, Glioma Malignant                                           2 (4%)       1 (2%)                                
      Olfactory Lobe, Neuroblastoma Malignant                                    2 (4%)                                             
   Spinal Cord                                                     (12)         (1)          (2)                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (50)         (90)         (50)         (50)                                   
      Adenocarcinoma                                   1 (2%)       1 (1%)                                                          
      Adenocarcinoma, Multiple                                                   1 (2%)                                             
      Adenocarcinoma, Metastatic, Harderian Gland      2 (4%)                    3 (6%)                                             
      Alveolar/Bronchiolar Adenoma                    14 (28%)      6 (7%)      11 (22%)      7 (14%)                               
      Alveolar/Bronchiolar Adenoma, Multiple          12 (24%)      4 (4%)       6 (12%)      5 (10%)                               
      Alveolar/Bronchiolar Carcinoma                   9 (18%)      5 (6%)      12 (24%)      6 (12%)                               
      Alveolar/Bronchiolar Carcinoma, Multiple         6 (12%)                   5 (10%)      5 (10%)                               
      Carcinoma, Metastatic, Preputial Gland                                     1 (2%)                                             
      Carcinoma, Metastatic, Zymbal's Gland                                      1 (2%)       1 (2%)                                
      Hemangiosarcoma, Metastatic, Heart              18 (36%)      2 (2%)       1 (2%)       3 (6%)                                
      Hemangiosarcoma, Metastatic, Liver               1 (2%)                    1 (2%)       1 (2%)                                
      Hepatocellular Carcinoma, Metastatic, Liver      1 (2%)                    5 (10%)                                            
      Histiocytic Sarcoma                              5 (10%)      3 (3%)       2 (4%)       2 (4%)                                
      Neoplasm NOS, Metastatic, Uncertain Primary                                                                                   
          Site                                                      1 (1%)                                                          
      Osteosarcoma, Metastatic, Uncertain Primary                                                                                   
          Site                                                                                1 (2%)                                
      Squamous Cell Carcinoma, Metastatic, Stomach     1 (2%)       1 (1%)       1 (2%)       1 (2%)                                
      Mediastinum, Adenocarcinoma, Metastatic, Lung                              1 (2%)                                             
      Mediastinum, Alveolar/Bronchiolar Carcinoma,                                                                                  
           Metastatic, Lung                                                      1 (2%)       1 (2%)                                
      Mediastinum, Carcinoma, Metastatic, Zymbal's                                                                                  
          Gland                                                                               1 (2%)                                
      Mediastinum, Hemangiosarcoma, Metastatic,                                                                                     
           Heart                                       1 (2%)                                 1 (2%)                                
      Mediastinum, Histiocytic Sarcoma                                           1 (2%)                                             
   Nose                                               (49)         (88)         (50)         (50)                                   
                                                             Page  22                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          312 PPM      625 PPM      625 PPM      625 PPM                                   
                                                   SE52                      SE13         SE 26                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
RESPIRATORY SYSTEM - cont                                                                                                           
      Histiocytic Sarcoma                                           1 (1%)                                                          
      Neuroblastoma Malignant, Metastatic, Brain                                 1 (2%)                                             
   Trachea                                            (47)         (84)         (48)         (48)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Eye                                                (4)          (6)          (4)          (3)                                    
      Adenocarcinoma, Metastatic, Harderian Gland      1 (25%)                                                                      
   Harderian Gland                                    (48)         (53)         (42)         (36)                                   
      Adenocarcinoma                                   1 (2%)                    4 (10%)                                            
      Adenoma                                         20 (42%)      7 (13%)     13 (31%)     10 (28%)                               
      Bilateral, Adenocarcinoma                        1 (2%)                                                                       
      Bilateral, Adenoma                               8 (17%)      2 (4%)       7 (17%)      3 (8%)                                
   Zymbal's Gland                                                               (3)          (2)                                    
      Carcinoma                                                                  2 (67%)      2 (100%)                              
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (49)         (90)         (50)         (50)                                   
      Adenocarcinoma, Metastatic, Pancreas             1 (2%)                                                                       
      Adenoma                                          1 (2%)       1 (1%)                                                          
      Hemangiosarcoma, Metastatic, Heart               6 (12%)                   1 (2%)       1 (2%)                                
      Histiocytic Sarcoma                              4 (8%)       4 (4%)       2 (4%)       2 (4%)                                
      Mast Cell Tumor NOS, Metastatic, Bone Marrow                                            1 (2%)                                
      Osteosarcoma, Metastatic, Uncertain Primary                                                                                   
          Site                                                                                1 (2%)                                
      Squamous Cell Carcinoma, Metastatic, Stomach                  1 (1%)       1 (2%)       1 (2%)                                
      Renal Tubule, Adenoma                            2 (4%)                    1 (2%)       1 (2%)                                
   Urinary Bladder                                    (47)         (83)         (46)         (48)                                   
      Hemangioma                                                                 1 (2%)                                             
      Histiocytic Sarcoma                                           2 (2%)                                                          
      Squamous Cell Carcinoma, Metastatic, Stomach                  1 (1%)                                                          
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SYSTEMIC LESIONS                                                                                                                   
   Multiple Organs                                   *(50)        *(90)        *(50)        *(50)                                   
      Histiocytic Sarcoma                              7 (14%)      4 (4%)       2 (4%)       2 (4%)                                
      Lymphoma Malignant                               1 (2%)       1 (1%)                                                          
      Lymphoma Malignant Lymphocytic                   4 (8%)      52 (58%)     17 (34%)     30 (60%)                               
      Lymphoma Malignant Mixed                         3 (6%)       1 (1%)       5 (10%)      3 (6%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
     *   Number of animals with any tissue examined microscopically                                                                 
                                                             Page  23                                                               
NTP Experiment-Test: 05134-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     1,3 BUTADIENE (BUTADIENE)                                 Date: 09/17/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 11:36:59  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          312 PPM      625 PPM      625 PPM      625 PPM                                   
                                                   SE52                      SE13         SE 26                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
TUMOR SUMMARY                                                                                                                       
                                                                                                                                    
   Total Animals with Primary Neoplasms (b)            50          74          49          49                                       
     Total Primary Neoplasms                          174         114         160         120                                       
                                                                                                                                    
   Total Animals with Benign Neoplasms                 41          21          34          22                                       
     Total Benign Neoplasms                            85          35          73          41                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms              50          66          49          49                                       
     Total Malignant Neoplasms                         88          79          87          78                                       
                                                                                                                                    
   Total Animals with Metastatic Neoplasms             33           6          16          13                                       
     Total Metastatic Neoplasm                         98          20          32          43                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms                           1                       1                                       
   Uncertain Primary Site                                                                                                           
                                                                                                                                    
   Total Animals with Neoplasms Uncertain-                                                                                          
   Benign or Malignant                                  1                                   1                                       
     Total Uncertain Neoplasms                          1                                   1                                       
                                                                                                                                    
____________________________________________________________________________________________________________________________________
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Primary tumors: all tumors except metastatic tumors                                                                              
                                                             Page  24                                                               
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                             ----------              END OF REPORT             ----------                                           
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NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.