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TDMS Study 05135-07 Pathology Tables

NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08
LAB: Battelle Northwest                                         ISOPRENE                                       DATE: 10/01/97

EXPERIMENT: 05135 TEST: 07                                                                                     TIME: 13:24:18
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                   PAGE:
CONT: NO1-ES-25335                              ROUTE: RESPIRATORY EXPOSURE WHOLE BODY                         NTP C#: 61825B
PATHOLOGIST: GRUMBEIN, S.                                                                                      CAS: 78-79-5
                

Rats(FISCHER344)
-----------------------------
FOR ALL DOSES THE TUMOR RATES IN THE FOLLOWING TISSUES/ORGANS ARE BASED ON NUMBER OF TISSUES EXAMINED.
IN OTHER TISSUES/ORGANS, RATES ARE BASED ON NUMBER OF ANIMALS NECROPSIED.

                                                 ----------------
                                                 Kidney
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08   
LAB: Battelle Northwest                                         ISOPRENE                                       DATE: 10/01/97     
EXPERIMENT: 05135 TEST: 07                                                                                     TIME: 13:24:18     
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                   PAGE:              
CONT: NO1-ES-25335                              ROUTE: RESPIRATORY EXPOSURE WHOLE BODY                         NTP C#: 61825B     
PATHOLOGIST: GRUMBEIN, S.                                                                                      CAS: 78-79-5       



SUMMARY OF STATISTICALLY SIGNIFICANT (P<=.05) RESULTS
IN THE ANALYSIS OF                     ISOPRENE
===============================================================

Male Rats
------------
Organ                                 Morphology
-----                                 ----------
Kidney, Renal Tubule (Step Section)    Adenoma
                                       Carcinoma and Adenoma
                                       Hyperplasia
                                       Hyperplasia and Adenoma
                                       Hyperplasia, Carcinoma or Adenoma
Kidney, Renal Tubule (Step and Original Sections)
                                       Adenoma
                                       Carcinoma and Adenoma
                                       Hyperplasia
                                       Hyperplasia and Adenoma
                                       Hyperplasia, Carcinoma or Adenoma
Kidney: Renal Tubule                   Adenoma
                                       Carcinoma or Adenoma
                                       Hyperplasia
                                       Hyperplasia and Adenoma
                                       Hyperplasia, Carcinoma or Adenoma
       Date:  10/01/97                           EXPERIMENT: 05135 TEST: 07                                            Page 1
                     Statistical Analysis of Primary Tumors in Rats(FISCHER344)        -                      ISOPRENE
                                         Terminal Sacrifice at 104 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     CONTROL      220 PPM      700 PPM      7000 PPM    |CONTROL      220 PPM      700 PPM      7000 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney, Renal Tubule (Step Section)                                                                                              |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     2/50 (4%)    2/50 (4%)    6/50 (12%)   10/50 (20%) |0/50 (0%)    0/50 (0%)    0/50 (0%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     5.4%         5.3%         16.0%        26.1%       |0.0%         0.0%         0.0%         0.0%         |
|TERMINAL (d)       |     0/18 (0%)    1/16 (6%)    4/15 (27%)   4/15 (27%)  |0/29 (0%)    0/30 (0%)    0/28 (0%)    0/27 (0%)    |
|FIRST INCIDENCE    |     704          702          566          622         |---          ---          ---          ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.004 **   P=0.637      P=0.093      P=0.013 *   |(e)          (e)          (e)          (e)          |
|POLY 3             |     P=0.004 **   P=0.688N     P=0.134      P=0.014 *   |(e)          (e)          (e)          (e)          |
|POLY 1.5           |     P=0.004 **   P=0.687N     P=0.137      P=0.013 *   |(e)          (e)          (e)          (e)          |
|POLY 6             |     P=0.005 **   P=0.696      P=0.123      P=0.014 *   |(e)          (e)          (e)          (e)          |
|LOGISTIC REGRESSION|     P=0.004 **   P=0.684      P=0.118      P=0.014 *   |(e)          (e)          (e)          (e)          |
|COCH-ARM / FISHERS |     P=0.005 **   P=0.691N     P=0.134      P=0.014 *   |(e)          (e)          (e)          (e)          |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     CONTROL      220 PPM      700 PPM      7000 PPM    |CONTROL      220 PPM      700 PPM      7000 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney, Renal Tubule (Step Section)                                                                                              |
|     Carcinoma and Adenoma                                                                                                       |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     2/50 (4%)    2/50 (4%)    6/50 (12%)   10/50 (20%) |0/50 (0%)    0/50 (0%)    0/50 (0%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     5.4%         5.3%         16.0%        26.1%       |0.0%         0.0%         0.0%         0.0%         |
|TERMINAL (d)       |     0/18 (0%)    1/16 (6%)    4/15 (27%)   4/15 (27%)  |0/29 (0%)    0/30 (0%)    0/28 (0%)    0/27 (0%)    |
|FIRST INCIDENCE    |     704          702          566          622         |---          ---          ---          ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.004 **   P=0.637      P=0.093      P=0.013 *   |(e)          (e)          (e)          (e)          |
|POLY 3             |     P=0.004 **   P=0.688N     P=0.134      P=0.014 *   |(e)          (e)          (e)          (e)          |
|POLY 1.5           |     P=0.004 **   P=0.687N     P=0.137      P=0.013 *   |(e)          (e)          (e)          (e)          |
|POLY 6             |     P=0.005 **   P=0.696      P=0.123      P=0.014 *   |(e)          (e)          (e)          (e)          |
|LOGISTIC REGRESSION|     P=0.004 **   P=0.684      P=0.118      P=0.014 *   |(e)          (e)          (e)          (e)          |
|COCH-ARM / FISHERS |     P=0.005 **   P=0.691N     P=0.134      P=0.014 *   |(e)          (e)          (e)          (e)          |
|=================================================================================================================================|
       Date:  10/01/97                           EXPERIMENT: 05135 TEST: 07                                            Page 2
                     Statistical Analysis of Primary Tumors in Rats(FISCHER344)       -                      ISOPRENE
                                         Terminal Sacrifice at 104 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     CONTROL      220 PPM      700 PPM      7000 PPM    |CONTROL      220 PPM      700 PPM      7000 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney, Renal Tubule (Step Section)                                                                                              |
|     Hyperplasia                                                                                                                 |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     7/50 (14%)   5/50 (10%)   9/50 (18%)   15/50 (30%) |0/50 (0%)    0/50 (0%)    0/50 (0%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     18.7%        13.2%        23.6%        38.2%       |0.0%         0.0%         0.0%         0.0%         |
|TERMINAL (d)       |     4/18 (22%)   3/16 (19%)   3/15 (20%)   4/15 (27%)  |0/29 (0%)    0/30 (0%)    0/28 (0%)    0/27 (0%)    |
|FIRST INCIDENCE    |     539          657          566          611         |---          ---          ---          ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.009 **   P=0.446N     P=0.303      P=0.038 *   |(e)          (e)          (e)          (e)          |
|POLY 3             |     P=0.007 **   P=0.370N     P=0.401      P=0.045 *   |(e)          (e)          (e)          (e)          |
|POLY 1.5           |     P=0.007 **   P=0.367N     P=0.406      P=0.042 *   |(e)          (e)          (e)          (e)          |
|POLY 6             |     P=0.009 **   P=0.385N     P=0.383      P=0.048 *   |(e)          (e)          (e)          (e)          |
|LOGISTIC REGRESSION|     P=0.007 **   P=0.377N     P=0.378      P=0.043 *   |(e)          (e)          (e)          (e)          |
|COCH-ARM / FISHERS |     P=0.008 **   P=0.380N     P=0.393      P=0.045 *   |(e)          (e)          (e)          (e)          |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     CONTROL      220 PPM      700 PPM      7000 PPM    |CONTROL      220 PPM      700 PPM      7000 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney, Renal Tubule (Step Section)                                                                                              |
|     Hyperplasia and Adenoma                                                                                                     |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     8/50 (16%)   6/50 (12%)   13/50 (26%)  18/50 (36%) |0/50 (0%)    0/50 (0%)    0/50 (0%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     21.3%        15.9%        34.1%        45.4%       |0.0%         0.0%         0.0%         0.0%         |
|TERMINAL (d)       |     4/18 (22%)   4/16 (25%)   7/15 (47%)   6/15 (40%)  |0/29 (0%)    0/30 (0%)    0/28 (0%)    0/27 (0%)    |
|FIRST INCIDENCE    |     539          657          566          611         |---          ---          ---          ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.005 **   P=0.466N     P=0.097      P=0.017 *   |(e)          (e)          (e)          (e)          |
|POLY 3             |     P=0.004 **   P=0.378N     P=0.154      P=0.018 *   |(e)          (e)          (e)          (e)          |
|POLY 1.5           |     P=0.004 **   P=0.375N     P=0.164      P=0.017 *   |(e)          (e)          (e)          (e)          |
|POLY 6             |     P=0.005 **   P=0.398N     P=0.131      P=0.019 *   |(e)          (e)          (e)          (e)          |
|LOGISTIC REGRESSION|     P=0.004 **   P=0.387N     P=0.135      P=0.018 *   |(e)          (e)          (e)          (e)          |
|COCH-ARM / FISHERS |     P=0.005 **   P=0.387N     P=0.163      P=0.020 *   |(e)          (e)          (e)          (e)          |
|=================================================================================================================================|
       Date:  10/01/97                           EXPERIMENT: 05135 TEST: 07                                            Page 3
                     Statistical Analysis of Primary Tumors in Rats(FISCHER344)       -                      ISOPRENE
                                         Terminal Sacrifice at 104 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     CONTROL      220 PPM      700 PPM      7000 PPM    |CONTROL      220 PPM      700 PPM      7000 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney, Renal Tubule (Step Section)                                                                                              |
|     Hyperplasia, Carcinoma or Adenoma                                                                                           |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     8/50 (16%)   6/50 (12%)   13/50 (26%)  18/50 (36%) |0/50 (0%)    0/50 (0%)    0/50 (0%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     21.3%        15.9%        34.1%        45.4%       |0.0%         0.0%         0.0%         0.0%         |
|TERMINAL (d)       |     4/18 (22%)   4/16 (25%)   7/15 (47%)   6/15 (40%)  |0/29 (0%)    0/30 (0%)    0/28 (0%)    0/27 (0%)    |
|FIRST INCIDENCE    |     539          657          566          611         |---          ---          ---          ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.005 **   P=0.466N     P=0.097      P=0.017 *   |(e)          (e)          (e)          (e)          |
|POLY 3             |     P=0.004 **   P=0.378N     P=0.154      P=0.018 *   |(e)          (e)          (e)          (e)          |
|POLY 1.5           |     P=0.004 **   P=0.375N     P=0.164      P=0.017 *   |(e)          (e)          (e)          (e)          |
|POLY 6             |     P=0.005 **   P=0.398N     P=0.131      P=0.019 *   |(e)          (e)          (e)          (e)          |
|LOGISTIC REGRESSION|     P=0.004 **   P=0.387N     P=0.135      P=0.018 *   |(e)          (e)          (e)          (e)          |
|COCH-ARM / FISHERS |     P=0.005 **   P=0.387N     P=0.163      P=0.020 *   |(e)          (e)          (e)          (e)          |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     CONTROL      220 PPM      700 PPM      7000 PPM    |CONTROL      220 PPM      700 PPM      7000 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney, Renal Tubule (Step and Original Sections)                                                                                |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     2/50 (4%)    4/50 (8%)    8/50 (16%)   15/50 (30%) |0/50 (0%)    0/50 (0%)    1/50 (2%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     5.4%         10.5%        21.0%        38.5%       |0.0%         0.0%         2.4%         0.0%         |
|TERMINAL (d)       |     0/18 (0%)    1/16 (6%)    4/15 (27%)   5/15 (33%)  |0/29 (0%)    0/30 (0%)    1/28 (4%)    0/27 (0%)    |
|FIRST INCIDENCE    |     704          559          566          622         |---          ---          734 (T)      ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P<0.001 **   P=0.287      P=0.034 *    P<0.001 **  |P=0.754N     (e)          P=0.493      (e)          |
|POLY 3             |     P<0.001 **   P=0.352      P=0.047 *    P<0.001 **  |P=0.750N     (e)          P=0.496      (e)          |
|POLY 1.5           |     P<0.001 **   P=0.350      P=0.048 *    P<0.001 **  |P=0.747N     (e)          P=0.496      (e)          |
|POLY 6             |     P<0.001 **   P=0.347      P=0.043 *    P<0.001 **  |P=0.752N     (e)          P=0.496      (e)          |
|LOGISTIC REGRESSION|     P<0.001 **   P=0.342      P=0.045 *    P<0.001 **  |(e)          (e)          P=0.493      (e)          |
|COCH-ARM / FISHERS |     P<0.001 **   P=0.339      P=0.046 *    P<0.001 **  |P=0.740N     (e)          P=0.500      (e)          |
|=================================================================================================================================|
       Date:  10/01/97                           EXPERIMENT: 05135 TEST: 07                                            Page 4
                     Statistical Analysis of Primary Tumors in Rats(FISCHER344)       -                      ISOPRENE
                                         Terminal Sacrifice at 104 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     CONTROL      220 PPM      700 PPM      7000 PPM    |CONTROL      220 PPM      700 PPM      7000 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney, Renal Tubule (Step and Original Sections)                                                                                |
|     Carcinoma and Adenoma                                                                                                       |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     2/50 (4%)    4/50 (8%)    8/50 (16%)   15/50 (30%) |0/50 (0%)    0/50 (0%)    1/50 (2%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     5.4%         10.5%        21.0%        38.5%       |0.0%         0.0%         2.4%         0.0%         |
|TERMINAL (d)       |     0/18 (0%)    1/16 (6%)    4/15 (27%)   5/15 (33%)  |0/29 (0%)    0/30 (0%)    1/28 (4%)    0/27 (0%)    |
|FIRST INCIDENCE    |     704          559          566          622         |---          ---          734 (T)      ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P<0.001 **   P=0.287      P=0.034 *    P<0.001 **  |P=0.754N     (e)          P=0.493      (e)          |
|POLY 3             |     P<0.001 **   P=0.352      P=0.047 *    P<0.001 **  |P=0.750N     (e)          P=0.496      (e)          |
|POLY 1.5           |     P<0.001 **   P=0.350      P=0.048 *    P<0.001 **  |P=0.747N     (e)          P=0.496      (e)          |
|POLY 6             |     P<0.001 **   P=0.347      P=0.043 *    P<0.001 **  |P=0.752N     (e)          P=0.496      (e)          |
|LOGISTIC REGRESSION|     P<0.001 **   P=0.342      P=0.045 *    P<0.001 **  |(e)          (e)          P=0.493      (e)          |
|COCH-ARM / FISHERS |     P<0.001 **   P=0.339      P=0.046 *    P<0.001 **  |P=0.740N     (e)          P=0.500      (e)          |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     CONTROL      220 PPM      700 PPM      7000 PPM    |CONTROL      220 PPM      700 PPM      7000 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney, Renal Tubule (Step and Original Sections)                                                                                |
|     Hyperplasia                                                                                                                 |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     7/50 (14%)   6/50 (12%)   13/50 (26%)  18/50 (36%) |4/50 (8%)    0/50 (0%)    0/50 (0%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     18.7%        15.9%        33.2%        45.0%       |9.4%         0.0%         0.0%         0.0%         |
|TERMINAL (d)       |     4/18 (22%)   4/16 (25%)   4/15 (27%)   5/15 (33%)  |4/29 (14%)   0/30 (0%)    0/28 (0%)    0/27 (0%)    |
|FIRST INCIDENCE    |     539          657          518          548         |734 (T)      ---          ---          ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.004 **   P=0.572N     P=0.080      P=0.010 *   |P=0.218N     P=0.058N     P=0.066N     P=0.071N     |
|POLY 3             |     P=0.003 **   P=0.493N     P=0.113      P=0.009 **  |P=0.210N     P=0.056N     P=0.062N     P=0.066N     |
|POLY 1.5           |     P=0.002 **   P=0.489N     P=0.113      P=0.008 **  |P=0.206N     P=0.058N     P=0.062N     P=0.065N     |
|POLY 6             |     P=0.004 **   P=0.513N     P=0.108      P=0.010 *   |P=0.214N     P=0.054N     P=0.062N     P=0.066N     |
|LOGISTIC REGRESSION|     P=0.003 **   P=0.504N     P=0.101      P=0.010 *   |P=0.218N     (e)          (e)          (e)          |
|COCH-ARM / FISHERS |     P=0.003 **   P=0.500N     P=0.105      P=0.010 *   |P=0.204N     P=0.059N     P=0.059N     P=0.059N     |
|=================================================================================================================================|
       Date:  10/01/97                           EXPERIMENT: 05135 TEST: 07                                            Page 5
                     Statistical Analysis of Primary Tumors in Rats(FISCHER344)       -                      ISOPRENE
                                         Terminal Sacrifice at 104 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     CONTROL      220 PPM      700 PPM      7000 PPM    |CONTROL      220 PPM      700 PPM      7000 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney, Renal Tubule (Step and Original Sections)                                                                                |
|     Hyperplasia and Adenoma                                                                                                     |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     8/50 (16%)   9/50 (18%)   19/50 (38%)  22/50 (44%) |4/50 (8%)    0/50 (0%)    1/50 (2%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     21.3%        23.4%        47.6%        54.5%       |9.4%         0.0%         2.4%         0.0%         |
|TERMINAL (d)       |     4/18 (22%)   5/16 (31%)   8/15 (53%)   7/15 (47%)  |4/29 (14%)   0/30 (0%)    1/28 (4%)    0/27 (0%)    |
|FIRST INCIDENCE    |     539          559          518          548         |734 (T)      ---          734 (T)      ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.004 **   P=0.414      P=0.008 **   P=0.003 **  |P=0.187N     P=0.058N     P=0.187N     P=0.071N     |
|POLY 3             |     P=0.002 **   P=0.520      P=0.010 *    P<0.001 **  |P=0.184N     P=0.056N     P=0.184N     P=0.066N     |
|POLY 1.5           |     P=0.002 **   P=0.520      P=0.011 *    P<0.001 **  |P=0.180N     P=0.058N     P=0.184N     P=0.065N     |
|POLY 6             |     P=0.003 **   P=0.505      P=0.009 **   P=0.002 **  |P=0.188N     P=0.054N     P=0.183N     P=0.066N     |
|LOGISTIC REGRESSION|     P=0.002 **   P=0.506      P=0.010 *    P=0.002 **  |P=0.187N     (e)          P=0.187N     (e)          |
|COCH-ARM / FISHERS |     P=0.003 **   P=0.500      P=0.012 *    P=0.002 **  |P=0.173N     P=0.059N     P=0.181N     P=0.059N     |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     CONTROL      220 PPM      700 PPM      7000 PPM    |CONTROL      220 PPM      700 PPM      7000 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney, Renal Tubule (Step and Original Sections)                                                                                |
|     Hyperplasia, Carcinoma or Adenoma                                                                                           |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     8/50 (16%)   9/50 (18%)   19/50 (38%)  22/50 (44%) |4/50 (8%)    0/50 (0%)    1/50 (2%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     21.3%        23.4%        47.6%        54.5%       |9.4%         0.0%         2.4%         0.0%         |
|TERMINAL (d)       |     4/18 (22%)   5/16 (31%)   8/15 (53%)   7/15 (47%)  |4/29 (14%)   0/30 (0%)    1/28 (4%)    0/27 (0%)    |
|FIRST INCIDENCE    |     539          559          518          548         |734 (T)      ---          734 (T)      ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.004 **   P=0.414      P=0.008 **   P=0.003 **  |P=0.187N     P=0.058N     P=0.187N     P=0.071N     |
|POLY 3             |     P=0.002 **   P=0.520      P=0.010 *    P<0.001 **  |P=0.184N     P=0.056N     P=0.184N     P=0.066N     |
|POLY 1.5           |     P=0.002 **   P=0.520      P=0.011 *    P<0.001 **  |P=0.180N     P=0.058N     P=0.184N     P=0.065N     |
|POLY 6             |     P=0.003 **   P=0.505      P=0.009 **   P=0.002 **  |P=0.188N     P=0.054N     P=0.183N     P=0.066N     |
|LOGISTIC REGRESSION|     P=0.002 **   P=0.506      P=0.010 *    P=0.002 **  |P=0.187N     (e)          P=0.187N     (e)          |
|COCH-ARM / FISHERS |     P=0.003 **   P=0.500      P=0.012 *    P=0.002 **  |P=0.173N     P=0.059N     P=0.181N     P=0.059N     |
|=================================================================================================================================|
       Date:  10/01/97                           EXPERIMENT: 05135 TEST: 07                                            Page 6
                     Statistical Analysis of Primary Tumors in Rats(FISCHER344)       -                      ISOPRENE
                                         Terminal Sacrifice at 104 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     CONTROL      220 PPM      700 PPM      7000 PPM    |CONTROL      220 PPM      700 PPM      7000 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney: Renal Tubule                                                                                                             |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     0/50 (0%)    2/50 (4%)    2/50 (4%)    6/50 (12%)  |0/50 (0%)    0/49 (0%)    1/50 (2%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     0.0%         5.3%         5.3%         15.8%       |0.0%         0.0%         2.4%         0.0%         |
|TERMINAL (d)       |     0/18 (0%)    0/16 (0%)    0/15 (0%)    1/15 (7%)   |0/29 (0%)    0/30 (0%)    1/28 (4%)    0/27 (0%)    |
|FIRST INCIDENCE    |     ---          559          566          630         |---          ---          734 (T)      ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.012 *    P=0.223      P=0.235      P=0.015 *   |P=0.754N     (e)          P=0.493      (e)          |
|POLY 3             |     P=0.009 **   P=0.244      P=0.242      P=0.016 *   |P=0.749N     (e)          P=0.496      (e)          |
|POLY 1.5           |     P=0.009 **   P=0.243      P=0.242      P=0.015 *   |P=0.746N     (e)          P=0.496      (e)          |
|POLY 6             |     P=0.009 **   P=0.242      P=0.238      P=0.016 *   |P=0.751N     (e)          P=0.496      (e)          |
|LOGISTIC REGRESSION|     P=0.010 *    P=0.234      P=0.231      P=0.017 *   |(e)          (e)          P=0.493      (e)          |
|COCH-ARM / FISHERS |     P=0.010 *    P=0.247      P=0.247      P=0.013 *   |P=0.739N     (e)          P=0.500      (e)          |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     CONTROL      220 PPM      700 PPM      7000 PPM    |CONTROL      220 PPM      700 PPM      7000 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney: Renal Tubule                                                                                                             |
|     Carcinoma or Adenoma                                                                                                        |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     0/50 (0%)    2/50 (4%)    2/50 (4%)    6/50 (12%)  |0/50 (0%)    0/49 (0%)    1/50 (2%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     0.0%         5.3%         5.3%         15.8%       |0.0%         0.0%         2.4%         0.0%         |
|TERMINAL (d)       |     0/18 (0%)    0/16 (0%)    0/15 (0%)    1/15 (7%)   |0/29 (0%)    0/30 (0%)    1/28 (4%)    0/27 (0%)    |
|FIRST INCIDENCE    |     ---          559          566          630         |---          ---          734 (T)      ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.012 *    P=0.223      P=0.235      P=0.015 *   |P=0.754N     (e)          P=0.493      (e)          |
|POLY 3             |     P=0.009 **   P=0.244      P=0.242      P=0.016 *   |P=0.749N     (e)          P=0.496      (e)          |
|POLY 1.5           |     P=0.009 **   P=0.243      P=0.242      P=0.015 *   |P=0.746N     (e)          P=0.496      (e)          |
|POLY 6             |     P=0.009 **   P=0.242      P=0.238      P=0.016 *   |P=0.751N     (e)          P=0.496      (e)          |
|LOGISTIC REGRESSION|     P=0.010 *    P=0.234      P=0.231      P=0.017 *   |(e)          (e)          P=0.493      (e)          |
|COCH-ARM / FISHERS |     P=0.010 *    P=0.247      P=0.247      P=0.013 *   |P=0.739N     (e)          P=0.500      (e)          |
|=================================================================================================================================|
       Date:  10/01/97                           EXPERIMENT: 05135 TEST: 07                                            Page 7
                     Statistical Analysis of Primary Tumors in Rats(FISCHER344)       -                      ISOPRENE
                                         Terminal Sacrifice at 104 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     CONTROL      220 PPM      700 PPM      7000 PPM    |CONTROL      220 PPM      700 PPM      7000 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney: Renal Tubule                                                                                                             |
|     Hyperplasia                                                                                                                 |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     0/50 (0%)    2/50 (4%)    6/50 (12%)   8/50 (16%)  |4/50 (8%)    0/49 (0%)    0/50 (0%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     0.0%         5.3%         15.6%        20.8%       |9.4%         0.0%         0.0%         0.0%         |
|TERMINAL (d)       |     0/18 (0%)    1/16 (6%)    2/15 (13%)   2/15 (13%)  |4/29 (14%)   0/30 (0%)    0/28 (0%)    0/27 (0%)    |
|FIRST INCIDENCE    |     ---          702          518          548         |734 (T)      ---          ---          ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.012 *    P=0.222      P=0.018 *    P=0.005 **  |P=0.218N     P=0.058N     P=0.066N     P=0.071N     |
|POLY 3             |     P=0.010 *    P=0.241      P=0.017 *    P=0.004 **  |P=0.210N     P=0.057N     P=0.062N     P=0.066N     |
|POLY 1.5           |     P=0.009 **   P=0.242      P=0.017 *    P=0.004 **  |P=0.206N     P=0.060N     P=0.062N     P=0.065N     |
|POLY 6             |     P=0.010 *    P=0.237      P=0.016 *    P=0.004 **  |P=0.215N     P=0.055N     P=0.062N     P=0.066N     |
|LOGISTIC REGRESSION|     P=0.010 *    P=0.226      P=0.018 *    P=0.005 **  |P=0.218N     (e)          (e)          (e)          |
|COCH-ARM / FISHERS |     P=0.010 *    P=0.247      P=0.013 *    P=0.003 **  |P=0.202N     P=0.061N     P=0.059N     P=0.059N     |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     CONTROL      220 PPM      700 PPM      7000 PPM    |CONTROL      220 PPM      700 PPM      7000 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney: Renal Tubule                                                                                                             |
|     Hyperplasia and Adenoma                                                                                                     |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     0/50 (0%)    4/50 (8%)    8/50 (16%)   12/50 (24%) |4/50 (8%)    0/49 (0%)    1/50 (2%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     0.0%         10.5%        20.4%        30.8%       |9.4%         0.0%         2.4%         0.0%         |
|TERMINAL (d)       |     0/18 (0%)    1/16 (6%)    2/15 (13%)   3/15 (20%)  |4/29 (14%)   0/30 (0%)    1/28 (4%)    0/27 (0%)    |
|FIRST INCIDENCE    |     ---          559          518          548         |734 (T)      ---          734 (T)      ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.002 **   P=0.057      P=0.006 **   P<0.001 **  |P=0.187N     P=0.058N     P=0.187N     P=0.071N     |
|POLY 3             |     P<0.001 **   P=0.064      P=0.004 **   P<0.001 **  |P=0.184N     P=0.057N     P=0.184N     P=0.066N     |
|POLY 1.5           |     P<0.001 **   P=0.064      P=0.004 **   P<0.001 **  |P=0.179N     P=0.060N     P=0.184N     P=0.065N     |
|POLY 6             |     P<0.001 **   P=0.062      P=0.004 **   P<0.001 **  |P=0.188N     P=0.055N     P=0.183N     P=0.066N     |
|LOGISTIC REGRESSION|     P<0.001 **   P=0.065      P=0.005 **   P<0.001 **  |P=0.187N     (e)          P=0.187N     (e)          |
|COCH-ARM / FISHERS |     P<0.001 **   P=0.059      P=0.003 **   P<0.001 **  |P=0.172N     P=0.061N     P=0.181N     P=0.059N     |
|=================================================================================================================================|
       Date:  10/01/97                           EXPERIMENT: 05135 TEST: 07                                            Page 8
                     Statistical Analysis of Primary Tumors in Rats(FISCHER344)       -                      ISOPRENE
                                         Terminal Sacrifice at 104 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     CONTROL      220 PPM      700 PPM      7000 PPM    |CONTROL      220 PPM      700 PPM      7000 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney: Renal Tubule                                                                                                             |
|     Hyperplasia, Carcinoma or Adenoma                                                                                           |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     0/50 (0%)    4/50 (8%)    8/50 (16%)   12/50 (24%) |4/50 (8%)    0/49 (0%)    1/50 (2%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     0.0%         10.5%        20.4%        30.8%       |9.4%         0.0%         2.4%         0.0%         |
|TERMINAL (d)       |     0/18 (0%)    1/16 (6%)    2/15 (13%)   3/15 (20%)  |4/29 (14%)   0/30 (0%)    1/28 (4%)    0/27 (0%)    |
|FIRST INCIDENCE    |     ---          559          518          548         |734 (T)      ---          734 (T)      ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.002 **   P=0.057      P=0.006 **   P<0.001 **  |P=0.187N     P=0.058N     P=0.187N     P=0.071N     |
|POLY 3             |     P<0.001 **   P=0.064      P=0.004 **   P<0.001 **  |P=0.184N     P=0.057N     P=0.184N     P=0.066N     |
|POLY 1.5           |     P<0.001 **   P=0.064      P=0.004 **   P<0.001 **  |P=0.179N     P=0.060N     P=0.184N     P=0.065N     |
|POLY 6             |     P<0.001 **   P=0.062      P=0.004 **   P<0.001 **  |P=0.188N     P=0.055N     P=0.183N     P=0.066N     |
|LOGISTIC REGRESSION|     P<0.001 **   P=0.065      P=0.005 **   P<0.001 **  |P=0.187N     (e)          P=0.187N     (e)          |
|COCH-ARM / FISHERS |     P<0.001 **   P=0.059      P=0.003 **   P<0.001 **  |P=0.172N     P=0.061N     P=0.181N     P=0.059N     |
|=================================================================================================================================|

(a)  Number of tumor-bearing animals /  number of animals examined at site.
(b)  Poly-3 adjusted lifetime tumor incidence.
(d)  Observed incidence at terminal kill.
(f)  Beneath the control incidence are the P-values associated with the trend 
test.  Beneath the dosed group incidence are the P-values corresponding to 
pairwise comparisons between the controls and that dosed group.  The life
table analysis regards tumors in animals dying prior to  terminal kill as
 being (directly or indirectly) the cause of death.
Logistic regression is an alternative
 method for analyzing the incidence of non-fatal tumors. The Cochran-Armitage
and Fishers exact tests compare directly the overall incidence rates
For all tests a negative trend is indicated by N
(e)  Value of Statistic cannot be computed.
(I)  Interim sacrifice
(T)  Terminal sacrifice
#   Tumor rates based on number of animals necropsied.
*   To the right of any statistical result, indicates significance at (P<=0.05).
**  To the right of any statistical result, indicates significance at (P<=0.01).
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.