National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/2962

TDMS Study 05135-07 Pathology Tables

NTP                                         STATISTICAL ANALYSIS OF SURVIVAL DATA                              REPORT: PEIRPT11     
LAB: Battelle Northwest                                         ISOPRENE                                       DATE: 09/24/97       
EXPERIMENT: 05135 TEST: 07                                                                                     TIME: 10:01:53       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                   PAGE:       1        
CONT: NO1-ES-25335                              ROUTE: RESPIRATORY EXPOSURE WHOLE BODY                         NTP C#: 61825B       
PATHOLOGIST: GRUMBEIN, S.                                                                                      CAS: 78-79-5         
------------------------------------------------------------------------------------------------------------------------------------
                                                                                                                                    
                                                           2 YEAR CHRONIC                                                           
                                                                                                                                    

               REASONS FOR REMOVAL:    ALL                                                                                          
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
               REMOVAL DATE RANGE:     ALL                                                                                          
                                                                                                                                    
               TREATMENT GROUPS:       INCLUDE ALL                                                                                  
NTP                                         STATISTICAL ANALYSIS OF SURVIVAL DATA                              REPORT: PEIRPT11    
LAB: Battelle Northwest                                         ISOPRENE                                       DATE: 09/24/97      
EXPERIMENT: 05135 TEST: 07                                                                                     TIME: 10:01:53      
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                       
CONT: NO1-ES-25335                              ROUTE: RESPIRATORY EXPOSURE WHOLE BODY                         NTP C#: 61825B      
PATHOLOGIST: GRUMBEIN, S.                                                                                      CAS: 78-79-5        
                                     ISOPRENE

                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                     MALE RATS
                       FIRST TERMINAL SACRIFICE AT 733 DAYS
                                                          

                          INDIVIDUAL SURVIVAL TIMES (DAYS)
 ==================================================================================
 |                                DOSE = CONTROL                                  |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 50     UNCENSORED DEATHS 32     CENSORED DEATHS 0    TERMINAL 18        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      235   342   440   454   468   482   502   518   539   547                 |
 |      551   555   564   592   597   600   604   652   652   659                 |
 |      664   671   672   675   686   694   704   721   723   723                 |
 |      724   728                                                                 |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                                DOSE = 220 PPM                                  |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 50     UNCENSORED DEATHS 34     CENSORED DEATHS 0    TERMINAL 16        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      398   420   455   499   510   535   538   549   559   581                 |
 |      588   592   597   602   604   629   635   652   657   657                 |
 |      657   664   670   671   682   693   698   702   702   703                 |
 |      714   717   719   723                                                     |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                                DOSE = 700 PPM                                  |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 50     UNCENSORED DEATHS 35     CENSORED DEATHS 0    TERMINAL 15        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      454   454   469   510   518   521   537   541   547   555                 |
 |      566   566   576   580   591   594   621   655   664   667                 |
 |      667   668   670   671   681   683   684   686   692   695                 |
 |      706   712   722   727   728                                               |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 ==================================================================================
                                     ISOPRENE

                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                     MALE RATS
                       FIRST TERMINAL SACRIFICE AT 733 DAYS
                                                          

                          INDIVIDUAL SURVIVAL TIMES (DAYS)
 ==================================================================================
 |                                DOSE = 7000 PPM                                 |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 50     UNCENSORED DEATHS 35     CENSORED DEATHS 0    TERMINAL 15        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      26    222   361   478   517   548   560   566   581   581                 |
 |      588   588   611   612   618   621   622   630   664   664                 |
 |      667   667   668   680   681   685   686   691   708   709                 |
 |      721   721   723   727   727                                               |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 ==================================================================================
                                     ISOPRENE

                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                     MALE RATS
                       FIRST TERMINAL SACRIFICE AT 733 DAYS

 ==================================================================================
 |               KAPLAN-MEIER SURVIVAL PROBABILITY ESTIMATES (%)                  |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |    DOSE                               TIME (DAYS)                              |
 |                 90   180   270   365   425   485   545   605   665   733 (A)   |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |           |                                                                    |
 |CONTROL    |   100.0 100.0  98.0  96.0  96.0  88.0  82.0  66.0  58.0  36.0      |
 |           |                                                                    |
 |           |                                                                    |
 |220 PPM    |   100.0 100.0 100.0 100.0  96.0  94.0  86.0  70.0  56.0  32.0      |
 |           |                                                                    |
 |           |                                                                    |
 |700 PPM    |   100.0 100.0 100.0 100.0 100.0  94.0  84.0  68.0  62.0  30.0      |
 |           |                                                                    |
 |           |                                                                    |
 |7000 PPM   |    98.0  98.0  96.0  94.0  94.0  92.0  90.0  76.0  60.0  30.0      |
 |           |                                                                    |
 |           |                                                                    |
 ==================================================================================
 ==================================================================================
 |                           SURVIVAL SUMMARY STATISTICS                          |
 |                                                                                |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                                                  DOSE                          |
 |                                                                                |
 |                                CONTROL      220 PPM      700 PPM      7000 PPM |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |SURVIVAL AT END OF STUDY|       36.0%        32.0%        30.0%        30.0%    |
 |(KAPLAN-MEIER)          |                                                       |
 |                        |                                                       |
 |SIGNIFICANCE (B)        |       P=0.944      P=0.847      P=0.726      P=0.807  |
 |(LIFE TABLE)            |                                                       |
 |                        |                                                       |
 |MEAN DAY OF             |       591.8        616.8        616.5        603.4    |
 |NATURAL DEATHS (C)      |       (21.0)       (15.1)       (14.0)       (24.6)   |
 |(STANDARD ERROR)        |                                                       |
 |                        |                                                       |
 |MEAN LIFE SPAN (D)      |       642.6        654.0        651.5        642.3    |
 |(STANDARD ERROR)        |       (16.5)       (12.8)       (12.4)       (19.1)   |
 |                        |                                                       |
 ==================================================================================
                                    

       (A)  FIRST TERMINAL SACRIFICE
       (B)  THE FIRST ENTRY IS THE TREND TEST (TARONE, 1975) RESULT.
            SUBSEQUENT ENTRIES ARE THE RESULTS OF PAIRWISE TESTS
            (COX,1972).  NEGATIVE TRENDS ARE INDICATED BY "N".
       (C)  MEAN OF ALL UNCENSORED DEATHS PRIOR TO TERMINAL SACRIFICE
       (D)  MEAN OF ALL DEATHS (UNCENSORED, CENSORED, TERMINAL SACRIFICE)
                                     ISOPRENE

                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                    FEMALE RATS
                       FIRST TERMINAL SACRIFICE AT 734 DAYS
                                                          

                          INDIVIDUAL SURVIVAL TIMES (DAYS)
 ==================================================================================
 |                                DOSE = CONTROL                                  |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 50     UNCENSORED DEATHS 21     CENSORED DEATHS 0    TERMINAL 29        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      398   454   482   539   552   580   580   596   629   630                 |
 |      648   657   657   670   671   684   705   714   727   727                 |
 |      728                                                                       |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                                DOSE = 220 PPM                                  |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 50     UNCENSORED DEATHS 20     CENSORED DEATHS 0    TERMINAL 30        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      370   482   587   601   602   622   626   638   643   664                 |
 |      670   670   685   686   693   694   695   714   727   727                 |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                                DOSE = 700 PPM                                  |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 50     UNCENSORED DEATHS 22     CENSORED DEATHS 0    TERMINAL 28        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      111   427   508   510   539   547   566   580   581   604                 |
 |      611   617   638   672   699   708   710   710   713   721                 |
 |      723   727                                                                 |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 ==================================================================================
                                     ISOPRENE

                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                    FEMALE RATS
                       FIRST TERMINAL SACRIFICE AT 734 DAYS
                                                          

                          INDIVIDUAL SURVIVAL TIMES (DAYS)
 ==================================================================================
 |                                DOSE = 7000 PPM                                 |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 50     UNCENSORED DEATHS 23     CENSORED DEATHS 0    TERMINAL 27        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      114   415   432   464   482   490   497   594   594   621                 |
 |      625   630   652   653   664   664   681   681   709   713                 |
 |      713   714   727                                                           |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 ==================================================================================
                                     ISOPRENE

                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                    FEMALE RATS
                       FIRST TERMINAL SACRIFICE AT 734 DAYS

 ==================================================================================
 |               KAPLAN-MEIER SURVIVAL PROBABILITY ESTIMATES (%)                  |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |    DOSE                               TIME (DAYS)                              |
 |                 90   180   270   365   425   485   545   605   665   734 (A)   |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |           |                                                                    |
 |CONTROL    |   100.0 100.0 100.0 100.0  98.0  94.0  92.0  84.0  74.0  58.0      |
 |           |                                                                    |
 |           |                                                                    |
 |220 PPM    |   100.0 100.0 100.0 100.0  98.0  96.0  96.0  90.0  80.0  60.0      |
 |           |                                                                    |
 |           |                                                                    |
 |700 PPM    |   100.0  98.0  98.0  98.0  98.0  96.0  90.0  80.0  74.0  56.0      |
 |           |                                                                    |
 |           |                                                                    |
 |7000 PPM   |   100.0  98.0  98.0  98.0  96.0  90.0  86.0  82.0  68.0  54.0      |
 |           |                                                                    |
 |           |                                                                    |
 ==================================================================================
 ==================================================================================
 |                           SURVIVAL SUMMARY STATISTICS                          |
 |                                                                                |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                                                  DOSE                          |
 |                                                                                |
 |                                CONTROL      220 PPM      700 PPM      7000 PPM |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |SURVIVAL AT END OF STUDY|       58.0%        60.0%        56.0%        54.0%    |
 |(KAPLAN-MEIER)          |                                                       |
 |                        |                                                       |
 |SIGNIFICANCE (B)        |       P=0.589      P=0.889N     P=0.930      P=0.736  |
 |(LIFE TABLE)            |                                                       |
 |                        |                                                       |
 |MEAN DAY OF             |       620.4        639.8        601.0        588.2    |
 |NATURAL DEATHS (C)      |       (20.4)       (19.2)       (29.6)       (29.7)   |
 |(STANDARD ERROR)        |                                                       |
 |                        |                                                       |
 |MEAN LIFE SPAN (D)      |       686.3        696.3        675.5        666.9    |
 |(STANDARD ERROR)        |       (11.6)       (10.0)       (16.0)       (17.0)   |
 |                        |                                                       |
 ==================================================================================
                                    

       (A)  FIRST TERMINAL SACRIFICE
       (B)  THE FIRST ENTRY IS THE TREND TEST (TARONE, 1975) RESULT.
            SUBSEQUENT ENTRIES ARE THE RESULTS OF PAIRWISE TESTS
            (COX,1972).  NEGATIVE TRENDS ARE INDICATED BY "N".
       (C)  MEAN OF ALL UNCENSORED DEATHS PRIOR TO TERMINAL SACRIFICE
       (D)  MEAN OF ALL DEATHS (UNCENSORED, CENSORED, TERMINAL SACRIFICE)
                                                              ISOPRENE
                                                    KAPLAN-MEIER SURVIVAL CURVES
                                                        CONTROL          = 1
                                                        220 PPM          = 2
                                                        700 PPM          = 3
                                                        7000 PPM         = 4

------------------------------------------------------ SPECIES=RATS SEX=MALE -------------------------------------------------------

                                         Plot of SURVIVAL*WEEK.  Symbol is value of DOSE.

      |
  104 +
      |
      | 1   4                                1                         2        3
      |     4                              4 1                1        2  2
   96 +                                    4                  1  4        2  1  2 3
      |                                                          4           1  1 3 4  23
      |                                                                         1 1 4  223
      |                                                                           1 1   23 3 2 4
   88 +                                                                             1  1   3 3 44
      |                                                                                1 1   23244
      |                                                                                  1   13224 4
      |                                                                                      1 12  2
P  80 +                                                                                         33 22
E     |                                                                                        11   2 2
R     |                                                                                         11 34 22 4
C     |                                                                                          1    1
E  72 +                                                                                            3  11244
N     |                                                                                                 2  2
T     |                                                                                               311 322
      |                                                                                                 1 342  1
S  64 +                                                                                                    4   3 3
U     |                                                                                                        1 13
R     |                                                                                                          44
V     |                                                                                                        2 11
I  56 +                                                                                                          22
V     |                                                                                                           114
A     |                                                                                                           211
L     |                                                                                                             121
   48 +                                                                                                              211
      |                                                                                                             4421 1
      |                                                                                                             33  411
      |                                                                                                              33
   40 +                                                                                                               3224
      |                                                                                                                322
      |                                                                                                                 341 1
      |                                                                                                                  22
   32 +                                                                                                                   2 2
      |                                                                                                                   3 3
      |
      |
   24 +
      |
      --+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+--
        0         10         20         30         40         50         60         70         80         90         100        110

                                                                   WEEK

NOTE: 38 obs hidden.
                                                              ISOPRENE
                                                    KAPLAN-MEIER SURVIVAL CURVES
                                                        CONTROL          = 1
                                                        220 PPM          = 2
                                                        700 PPM          = 3
                                                        7000 PPM         = 4

----------------------------------------------------- SPECIES=RATS SEX=FEMALE ------------------------------------------------------

                                         Plot of SURVIVAL*WEEK.  Symbol is value of DOSE.

      |
      |
  104 +
      |
      | 1                 34                                      2    1
      |                   34                                      2    1  43    1   2
   96 +                                                                   434   1   1   3           2
      |                                                                     4     4 1        1      2  2
P     |                                                                           4 4   3    1 1
E     |                                                                             44       3 1   1   2  2
R  88 +                                                                              44        3 3        22
C     |                                                                               4          3 1  41   2 2
E     |                                                                                                1   1
N     |                                                                                            3  4 3 4  2   2
T  80 +                                                                                                 3341  1  22
      |                                                                                                  33   11
S     |                                                                                                   34 3 4  2 2
U     |                                                                                                      3 1  1
R  72 +                                                                                                        4 43 223
V     |                                                                                                           1 122 3
I     |                                                                                                          4  1  1
V     |                                                                                                               211
A  64 +                                                                                                             4   1 1
L     |                                                                                                                 33
      |                                                                                                                  32 2
      |                                                                                                                   1 1
   56 +                                                                                                                 4 3 3
      |                                                                                                                   4 4
      |
      |
   48 +
      |
      --+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+--
        0         10         20         30         40         50         60         70         80         90         100        110

                                                                   WEEK

NOTE: 17 obs hidden.
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.