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TDMS Study 88036-04 Pathology Tables

NTP Experiment-Test: 88036-04         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           ANTHRAQUINONE                                       Date: 05/27/99
Route: DOSED FEED                                                                                                 Time: 11:24:12

                                                    FINAL #2 CORE STUDY MICE




       Facility:  Battelle Columbus Laboratory

       Chemical CAS #:  84-65-1

       Lock Date:  07/01/97

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All


































a  Number of animals examined microscopically at site and number of animals with lesion

                                                              Page   1


NTP Experiment-Test: 88036-04         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           ANTHRAQUINONE                                       Date: 05/27/99  
Route: DOSED FEED                                                                                                 Time: 11:24:12  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE FEMALE                        0 PPM        833 PPM      2500 PPM     7500 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________

DISPOSITION SUMMARY                                                                                                                 


  Animals Initially In Study                          50           50           50           50                                     
  Early Deaths                                                                                                                      
    Natural Death                                      9            5            8            5                                     
    Moribund Sacrifice                                 6            3            4            2                                     
    Accidently Killed                                                            3                                                  
  Survivors                                                                                                                         
    Terminal Sacrifice                                35           42           35           42                                     
  Missing                                                                                     1                                     

  Animals Examined Microscopically                    50           50           50           49                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Esophagus                                          (50)         (49)         (50)         (49)                                   
      Inflammation, Chronic                            1 (2%)                                                                       
   Intestine Small, Duodenum                          (50)         (50)         (50)         (48)                                   
      Ulcer                                            1 (2%)                    1 (2%)                                             
   Liver                                              (49)         (50)         (50)         (49)                                   
      Basophilic Focus                                 1 (2%)       1 (2%)                    3 (6%)                                
      Clear Cell Focus                                 4 (8%)       1 (2%)       1 (2%)       3 (6%)                                
      Degeneration, Diffuse, Fatty                     1 (2%)       1 (2%)                                                          
      Degeneration, Fatty, Focal                       2 (4%)       3 (6%)       1 (2%)       9 (18%)                               
      Eosinophilic Focus                               6 (12%)     15 (30%)     11 (22%)     22 (45%)                               
      Fatty Change, Focal                              5 (10%)      2 (4%)       1 (2%)       3 (6%)                                
      Hematopoietic Cell Proliferation                 1 (2%)       1 (2%)       3 (6%)       1 (2%)                                
      Infiltration Cellular, Lymphocyte                                                       3 (6%)                                
      Mineralization, Focal                                                                   1 (2%)                                
      Mixed Cell Focus                                 4 (8%)       2 (4%)       2 (4%)       2 (4%)                                
      Necrosis, Focal                                  5 (10%)      3 (6%)       1 (2%)       1 (2%)                                
      Tension Lipidosis                                1 (2%)                                                                       
      Bile Duct, Cyst                                  3 (6%)       2 (4%)       2 (4%)                                             
      Bile Duct, Hyperplasia                                                     1 (2%)                                             
      Centrilobular, Atrophy                           1 (2%)                                                                       
      Centrilobular, Degeneration, Fatty               1 (2%)                                 1 (2%)                                
      Centrilobular, Hypertrophy                       1 (2%)      27 (54%)     22 (44%)     39 (80%)                               
      Hepatocyte, Erythrophagocytosis                  1 (2%)                                                                       
      Serosa, Inflammation, Chronic                    1 (2%)                                                                       
   Mesentery                                          (6)          (4)          (6)          (7)                                    
      Inflammation, Chronic                            1 (17%)                                                                      
      Inflammation, Chronic Active                                               1 (17%)                                            
      Artery, Thrombosis                               1 (17%)                                                                      
      Fat, Necrosis                                    4 (67%)      3 (75%)      2 (33%)      7 (100%)                              
      Lymphatic, Angiectasis                                                     1 (17%)                                            
   Pancreas                                           (50)         (50)         (50)         (49)                                   
      Hyperplasia, Focal                                            2 (4%)                                                          

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 88036-04         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           ANTHRAQUINONE                                       Date: 05/27/99  
Route: DOSED FEED                                                                                                 Time: 11:24:12  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE FEMALE                        0 PPM        833 PPM      2500 PPM     7500 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - CONT                                                                                                            
      Inflammation, Chronic                            1 (2%)                                                                       
      Acinus, Atrophy                                  2 (4%)       1 (2%)       2 (4%)       1 (2%)                                
      Duct, Cyst                                                                 1 (2%)       1 (2%)                                
   Stomach, Forestomach                               (50)         (50)         (50)         (49)                                   
      Inflammation, Suppurative                                                               1 (2%)                                
      Ulcer                                            4 (8%)       1 (2%)       1 (2%)       2 (4%)                                
      Epithelium, Hyperplasia, Focal                                1 (2%)       1 (2%)       1 (2%)                                
      Serosa, Inflammation, Chronic                    1 (2%)                                                                       
   Stomach, Glandular                                 (50)         (50)         (50)         (49)                                   
      Ulcer                                            1 (2%)                    1 (2%)                                             
      Epithelium, Hyperplasia                                       2 (4%)                                                          
      Epithelium, Hyperplasia, Focal                                                          1 (2%)                                
      Serosa, Inflammation, Chronic                    1 (2%)                                                                       
   Tooth                                              (1)                                                                           
      Peridontal Tissue, Inflammation,                                                                                              
          Granulomatous                                1 (100%)                                                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Blood Vessel                                       (50)         (50)         (49)         (49)                                   
      Aorta, Mineralization                            2 (4%)                    1 (2%)                                             
   Heart                                              (50)         (50)         (50)         (49)                                   
      Thrombosis                                                    1 (2%)                                                          
      Myocardium, Degeneration                         2 (4%)                    1 (2%)       1 (2%)                                
      Myocardium, Mineralization                       2 (4%)                                                                       
      Valve, Inflammation                                           1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (50)         (50)         (50)         (49)                                   
      Hyperplasia, Focal                                            1 (2%)       1 (2%)       2 (4%)                                
      Necrosis                                                      1 (2%)                                                          
   Adrenal Medulla                                    (50)         (50)         (49)         (49)                                   
      Hyperplasia, Focal                                                                      1 (2%)                                
   Islets, Pancreatic                                 (50)         (50)         (50)         (49)                                   
      Hyperplasia                                      6 (12%)     13 (26%)     10 (20%)     14 (29%)                               
   Pituitary Gland                                    (47)         (50)         (48)         (46)                                   
      Angiectasis                                      2 (4%)                    1 (2%)                                             
      Atrophy                                                       1 (2%)                                                          
      Cyst                                                                                    1 (2%)                                
      Pars Distalis, Hyperplasia, Focal                8 (17%)      4 (8%)      12 (25%)      6 (13%)                               
   Thyroid Gland                                      (45)         (48)         (48)         (48)                                   
      Inflammation, Acute, Focal                       1 (2%)                                                                       
      Follicle, Cyst                                   2 (4%)       1 (2%)                                                          
      Follicular Cell, Hyperplasia                    10 (22%)     14 (29%)     16 (33%)     15 (31%)                               

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 88036-04         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           ANTHRAQUINONE                                       Date: 05/27/99  
Route: DOSED FEED                                                                                                 Time: 11:24:12  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE FEMALE                        0 PPM        833 PPM      2500 PPM     7500 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   Peritoneum                                         (1)                                                                           
      Necrosis                                         1 (100%)                                                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Ovary                                              (45)         (50)         (49)         (49)                                   
      Angiectasis                                                                             2 (4%)                                
      Cyst                                             3 (7%)       4 (8%)       7 (14%)      5 (10%)                               
      Granulosa Cell, Hyperplasia                      1 (2%)                                                                       
   Uterus                                             (50)         (50)         (50)         (49)                                   
      Angiectasis                                      2 (4%)                    1 (2%)       1 (2%)                                
      Inflammation, Chronic                            1 (2%)                                                                       
      Inflammation, Suppurative                        1 (2%)                    1 (2%)                                             
      Thrombosis                                                                 1 (2%)                                             
      Endometrium, Hyperplasia, Cystic                35 (70%)     38 (76%)     23 (46%)     39 (80%)                               
   Vagina                                                          (1)                                                              
      Hemorrhage                                                    1 (100%)                                                        
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (50)         (50)         (50)         (49)                                   
      Myeloid Cell, Hyperplasia                                                               1 (2%)                                
   Lymph Node                                         (5)          (2)          (4)          (4)                                    
      Lumbar, Ectasia                                               1 (50%)                                                         
      Lumbar, Hemorrhage                                                         1 (25%)                                            
      Mediastinal, Inflammation, Chronic               1 (20%)                                                                      
   Lymph Node, Mandibular                             (48)         (46)         (48)         (49)                                   
      Hyperplasia, Lymphoid                                         1 (2%)                                                          
      Inflammation, Suppurative                        1 (2%)                                                                       
   Lymph Node, Mesenteric                             (48)         (50)         (49)         (48)                                   
      Ectasia                                                                                 1 (2%)                                
   Spleen                                             (45)         (49)         (48)         (48)                                   
      Hematopoietic Cell Proliferation                 9 (20%)     17 (35%)     17 (35%)     26 (54%)                               
      Pigmentation                                     1 (2%)                                                                       
      Capsule, Inflammation, Chronic                   1 (2%)                                                                       
      Lymphoid Follicle, Hyperplasia                   8 (18%)      4 (8%)       5 (10%)      6 (13%)                               
   Thymus                                             (44)         (47)         (46)         (44)                                   
      Hyperplasia, Lymphoid                            1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 88036-04         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           ANTHRAQUINONE                                       Date: 05/27/99  
Route: DOSED FEED                                                                                                 Time: 11:24:12  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE FEMALE                        0 PPM        833 PPM      2500 PPM     7500 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (48)         (48)         (48)         (49)                                   
      Inflammation, Suppurative                        1 (2%)                                                                       
   Skin                                               (50)         (50)         (50)         (49)                                   
      Hair Follicle, Atrophy, Focal                                              1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (50)         (50)         (50)         (49)                                   
      Fibrosis                                         2 (4%)       2 (4%)       1 (2%)       3 (6%)                                
   Skeletal Muscle                                    (3)          (2)          (2)          (1)                                    
      Hemorrhage, Acute                                1 (33%)                                                                      
      Inflammation, Chronic                            1 (33%)                                                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (50)         (50)         (50)         (49)                                   
      Hemorrhage                                                                              1 (2%)                                
      Hydrocephalus                                                              1 (2%)                                             
      Hypothalamus, Compression                                     1 (2%)       1 (2%)       1 (2%)                                
      Hypothalamus, Degeneration                       1 (2%)                                                                       
      Hypothalamus, Necrosis                                        1 (2%)                                                          
   Peripheral Nerve                                   (2)                       (1)                                                 
      Axon, Degeneration                               2 (100%)                                                                     
   Spinal Cord                                        (2)                       (1)                                                 
      Axon, Nerve, Degeneration                        1 (50%)                                                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (50)         (50)         (50)         (49)                                   
      Congestion                                                                 2 (4%)                                             
      Hematopoietic Cell Proliferation                                                        1 (2%)                                
      Hemorrhage                                                    2 (4%)       1 (2%)                                             
      Hemorrhage, Chronic, Focal                       1 (2%)                                                                       
      Inflammation, Chronic                                                      1 (2%)                                             
      Alveolar Epithelium, Hyperplasia, Focal                                    2 (4%)                                             
      Bronchus, Foreign Body                           1 (2%)                                                                       
      Interstitium, Inflammation, Chronic, Focal                                              1 (2%)                                
      Interstitium, Mineralization                     1 (2%)                                                                       
      Perivascular, Edema                                                        1 (2%)                                             
   Nose                                               (50)         (50)         (49)         (49)                                   
      Inflammation, Suppurative                        2 (4%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 88036-04         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           ANTHRAQUINONE                                       Date: 05/27/99  
Route: DOSED FEED                                                                                                 Time: 11:24:12  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE FEMALE                        0 PPM        833 PPM      2500 PPM     7500 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Eye                                                (2)          (3)          (1)          (1)                                    
      Degeneration                                     1 (50%)      1 (33%)      1 (100%)     1 (100%)                              
      Cornea, Inflammation, Chronic Active                          1 (33%)                                                         
      Lens, Cataract                                   1 (50%)                                                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (49)         (50)         (49)         (49)                                   
      Glomerulosclerosis                               1 (2%)                                                                       
      Infarct                                                       2 (4%)                                                          
      Inflammation, Suppurative                        1 (2%)       1 (2%)                                                          
      Nephropathy                                      4 (8%)       3 (6%)       3 (6%)       7 (14%)                               
      Pigmentation                                     1 (2%)                                                                       
      Pelvis, Inflammation, Chronic Active             1 (2%)                                                                       
      Renal Tubule, Cyst                                                                      1 (2%)                                
      Renal Tubule, Hyperplasia, Focal                                           1 (2%)       1 (2%)                                
   Urinary Bladder                                    (44)         (48)         (46)         (48)                                   
      Edema                                                                                   1 (2%)                                
      Transitional Epithelium, Inclusion Body                                                                                       
          Intracytoplasmic                                         40 (83%)     43 (93%)     46 (96%)                               
                                                                                                                                    

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 88036-04         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           ANTHRAQUINONE                                       Date: 05/27/99  
Route: DOSED FEED                                                                                                 Time: 11:24:12  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE MALE                          0 PPM        833 PPM      2500 PPM     7500 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________

DISPOSITION SUMMARY                                                                                                                 


  Animals Initially In Study                          50           50           50           50                                     
  Early Deaths                                                                                                                      
    Natural Death                                      2            6            4           19                                     
    Moribund Sacrifice                                 3            3            3            8                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                45           41           43           23                                     

  Animals Examined Microscopically                    50           50           50           50                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Gallbladder                                        (50)         (48)         (47)         (48)                                   
      Cyst                                                                       1 (2%)                                             
   Liver                                              (50)         (50)         (50)         (49)                                   
      Basophilic Focus                                              3 (6%)       1 (2%)                                             
      Clear Cell Focus                                14 (28%)     12 (24%)      9 (18%)      2 (4%)                                
      Degeneration, Fatty, Focal                                    7 (14%)      6 (12%)                                            
      Eosinophilic Focus                              14 (28%)     17 (34%)     24 (48%)     20 (41%)                               
      Fatty Change, Focal                              1 (2%)                                 1 (2%)                                
      Hematopoietic Cell Proliferation                              2 (4%)                    4 (8%)                                
      Infarct                                                                                 1 (2%)                                
      Inflammation, Granulomatous                                   1 (2%)                                                          
      Mineralization                                                                          2 (4%)                                
      Mixed Cell Focus                                 3 (6%)       2 (4%)       4 (8%)       1 (2%)                                
      Necrosis, Focal                                  2 (4%)       3 (6%)       3 (6%)       8 (16%)                               
      Bile Duct, Cyst                                  1 (2%)       2 (4%)                    1 (2%)                                
      Centrilobular, Degeneration, Fatty                                         1 (2%)                                             
      Centrilobular, Hypertrophy                      24 (48%)     34 (68%)     41 (82%)     33 (67%)                               
      Hepatocyte, Erythrophagocytosis                  1 (2%)       9 (18%)     13 (26%)      6 (12%)                               
   Mesentery                                          (2)          (2)          (1)          (5)                                    
      Fat, Necrosis                                    2 (100%)     2 (100%)                  5 (100%)                              
   Pancreas                                           (50)         (50)         (50)         (50)                                   
      Inflammation, Acute                                           1 (2%)                                                          
      Acinus, Atrophy                                  2 (4%)       1 (2%)                    1 (2%)                                
      Duct, Necrosis                                                1 (2%)                                                          
   Stomach, Forestomach                               (50)         (49)         (50)         (50)                                   
      Cyst                                                                       1 (2%)                                             
      Ulcer                                                         1 (2%)                    2 (4%)                                
      Epithelium, Hyperplasia, Focal                                                          2 (4%)                                
   Stomach, Glandular                                 (50)         (50)         (50)         (50)                                   
      Foreign Body                                                  1 (2%)                                                          
      Inflammation, Focal, Suppurative                              1 (2%)                                                          
      Metaplasia, Squamous                             1 (2%)                                                                       
      Mineralization                                                                          1 (2%)                                
      Ulcer                                            2 (4%)                                 1 (2%)                                

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 88036-04         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           ANTHRAQUINONE                                       Date: 05/27/99  
Route: DOSED FEED                                                                                                 Time: 11:24:12  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE MALE                          0 PPM        833 PPM      2500 PPM     7500 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - CONT                                                                                                            
      Epithelium, Hyperplasia, Focal                   1 (2%)                                                                       
   Tooth                                              (14)         (19)         (14)         (5)                                    
      Inflammation, Suppurative                                     1 (5%)       1 (7%)                                             
      Malformation                                    14 (100%)    19 (100%)    14 (100%)     5 (100%)                              
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (50)         (50)         (50)         (50)                                   
      Atrium, Thrombosis                               2 (4%)                                                                       
      Myocardium, Degeneration                         4 (8%)                    3 (6%)       5 (10%)                               
      Myocardium, Mineralization                                                 1 (2%)       1 (2%)                                
      Valve, Fibrosis                                  1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (50)         (50)         (50)         (50)                                   
      Hyperplasia, Focal                               8 (16%)      8 (16%)      8 (16%)      5 (10%)                               
      Subcapsular, Hyperplasia, Focal                  1 (2%)       1 (2%)       1 (2%)                                             
   Adrenal Medulla                                    (49)         (50)         (50)         (49)                                   
      Hyperplasia, Focal                               2 (4%)                                                                       
   Islets, Pancreatic                                 (50)         (50)         (50)         (42)                                   
      Hyperplasia                                     40 (80%)     40 (80%)     29 (58%)     17 (40%)                               
   Pituitary Gland                                    (48)         (48)         (47)         (46)                                   
      Pars Distalis, Hyperplasia, Focal                1 (2%)                    2 (4%)                                             
   Thyroid Gland                                      (50)         (50)         (49)         (46)                                   
      Follicular Cell, Hyperplasia                     7 (14%)     10 (20%)     15 (31%)     21 (46%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Epididymis                                         (50)         (50)         (50)         (50)                                   
      Granuloma Sperm                                                                         3 (6%)                                
      Hemorrhage                                                                 1 (2%)                                             
   Preputial Gland                                    (50)         (50)         (49)         (49)                                   
      Cyst                                            22 (44%)     25 (50%)     32 (65%)     22 (45%)                               
      Inflammation, Granulomatous                                   4 (8%)                    1 (2%)                                
      Inflammation, Suppurative                        7 (14%)      4 (8%)       4 (8%)       4 (8%)                                
   Prostate                                           (50)         (50)         (50)         (50)                                   
      Hyperplasia                                                   1 (2%)                                                          
   Seminal Vesicle                                    (50)         (50)         (50)         (50)                                   

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 88036-04         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           ANTHRAQUINONE                                       Date: 05/27/99  
Route: DOSED FEED                                                                                                 Time: 11:24:12  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE MALE                          0 PPM        833 PPM      2500 PPM     7500 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
GENITAL SYSTEM - CONT                                                                                                               
      Dilatation                                       1 (2%)                                                                       
      Inflammation, Chronic                            7 (14%)      3 (6%)       4 (8%)                                             
   Testes                                             (50)         (49)         (50)         (50)                                   
      Germinal Epithelium, Degeneration                                          2 (4%)       3 (6%)                                
      Germinal Epithelium, Mineralization              2 (4%)                    1 (2%)       1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Lymph Node                                         (1)          (1)          (3)                                                 
      Hyperplasia, Lymphoid                                         1 (100%)     1 (33%)                                            
   Lymph Node, Mesenteric                             (49)         (49)         (47)         (40)                                   
      Angiectasis                                                                             1 (3%)                                
      Erythrophagocytosis                                           1 (2%)                                                          
      Hyperplasia, Lymphoid                            1 (2%)                                                                       
   Spleen                                             (50)         (50)         (49)         (42)                                   
      Atrophy                                          1 (2%)       1 (2%)                                                          
      Hematopoietic Cell Proliferation                12 (24%)     14 (28%)     12 (24%)     30 (71%)                               
      Infiltration Cellular, Mast Cell                 1 (2%)                                                                       
      Pigmentation                                                               1 (2%)       1 (2%)                                
      Lymphoid Follicle, Atrophy                                                              1 (2%)                                
      Lymphoid Follicle, Hyperplasia                   4 (8%)                    1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Skin                                               (50)         (50)         (50)         (49)                                   
      Ulcer                                                         1 (2%)       1 (2%)                                             
      Conjunctiva, Inflammation, Chronic                                         1 (2%)                                             
      Prepuce, Hemorrhage                              1 (2%)                                                                       
      Subcutaneous Tissue, Edema                       1 (2%)       1 (2%)                                                          
      Subcutaneous Tissue, Inflammation, Chronic                                                                                    
          Active                                                                 1 (2%)                                             
      Subcutaneous Tissue, Inflammation,                                                                                            
          Suppurative                                  1 (2%)                                                                       
      Subcutaneous Tissue, Mineralization              1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 88036-04         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           ANTHRAQUINONE                                       Date: 05/27/99  
Route: DOSED FEED                                                                                                 Time: 11:24:12  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE MALE                          0 PPM        833 PPM      2500 PPM     7500 PPM                                  
                                                                                                                                    
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 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (50)         (50)         (50)         (50)                                   
      Meninges, Infiltration Cellular, Lymphocyte      1 (2%)                                                                       
   Peripheral Nerve                                                             (1)                                                 
      Axon, Degeneration                                                         1 (100%)                                           
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 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (50)         (50)         (50)         (50)                                   
      Inflammation, Chronic                                         1 (2%)                                                          
      Inflammation, Granulomatous                                                1 (2%)                                             
      Alveolar Epithelium, Hyperplasia, Focal          1 (2%)       1 (2%)       1 (2%)                                             
      Vein, Thrombosis                                              1 (2%)                                                          
   Nose                                               (50)         (50)         (50)         (50)                                   
      Inflammation, Suppurative                        2 (4%)       3 (6%)       1 (2%)       2 (4%)                                
   Trachea                                            (50)         (50)         (50)         (50)                                   
      Glands, Hyperplasia                                           1 (2%)                                                          
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 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Eye                                                (1)          (2)                                                              
      Degeneration                                                  1 (50%)                                                         
      Cornea, Inflammation, Chronic                    1 (100%)     1 (50%)                                                         
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 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (50)         (49)         (49)         (47)                                   
      Degeneration                                     1 (2%)                                                                       
      Infarct                                          1 (2%)                                                                       
      Metaplasia, Osseous                                                        1 (2%)                                             
      Necrosis                                                      1 (2%)                                                          
      Nephropathy                                     29 (58%)     25 (51%)     27 (55%)     18 (38%)                               
      Pigmentation                                                  2 (4%)       2 (4%)      18 (38%)                               
      Glomerulus, Inflammation, Chronic                1 (2%)                                                                       
      Pelvis, Inflammation, Suppurative                             1 (2%)                                                          
      Renal Tubule, Cyst                               2 (4%)                                                                       
      Renal Tubule, Hyperplasia, Focal                              1 (2%)       1 (2%)                                             
      Renal Tubule, Pigmentation, Lipofuscin                                                  1 (2%)                                
      Renal Tubule, Vacuolization Cytoplasmic                                                 1 (2%)                                
   Urinary Bladder                                    (50)         (49)         (49)         (45)                                   
      Calculus Gross Observation                                    1 (2%)                                                          
      Transitional Epithelium, Inclusion Body                                                                                       

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 88036-04         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           ANTHRAQUINONE                                       Date: 05/27/99  
Route: DOSED FEED                                                                                                 Time: 11:24:12  
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         B6C3F1 MICE MALE                          0 PPM        833 PPM      2500 PPM     7500 PPM                                  
                                                                                                                                    
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URINARY SYSTEM - CONT                                                                                                               
          Intracytoplasmic                                         46 (94%)     46 (94%)     42 (93%)                               
                                                                                                                                    

a  Number of animals examined microscopically at site and number of animals with lesion                                             
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