Skip to Main Navigation
Skip to Page Content
Share This:
https://ntp.niehs.nih.gov/go/4790

TDMS Study 88036-04 Pathology Tables

NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: Battelle Columbus                                       ANTHRAQUINONE                                     DATE: 12/11/98       
EXPERIMENT: 88036 TEST: 04                                                                                     TIME: 09:00:07       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                   PAGE:       1        
CONT: NO1-ES-25337                              ROUTE: DOSED FEED                                              NTP C#: C88036       
PATHOLOGIST: RYAN, M.                                                                                          CAS: 84-65-1         
------------------------------------------------------------------------------------------------------------------------------------
                                                                                                                                    
                                                        2 YEAR CHRONIC MICE                                                         
                                                                                                                                    

               REASONS FOR REMOVAL:    ALL                                                                                          
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
               REMOVAL DATE RANGE:     ALL                                                                                          
                                                                                                                                    
               TREATMENT GROUPS:       INCLUDE ALL                                                                                  
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08
LAB: Battelle Columbus                                       ANTHRAQUINONE                                     DATE: 12/11/98

EXPERIMENT: 88036 TEST: 04                                                                                     TIME: 09:00:07
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE
CONT: NO1-ES-25337                              ROUTE: DOSED FEED                                              NTP C#: C88036
PATHOLOGIST: RYAN, M.                                                                                          CAS: 84-65-1

Mice(B6C3F1)
-----------------------------
FOR ALL DOSES THE TUMOR RATES IN THE FOLLOWING TISSUES/ORGANS ARE
BASED ON NUMBER OF TISSUES EXAMINED.  IN OTHER TISSUES/ORGANS RATES
ARE BASED ON THE NUMBER OF ANIMALS NECROPSIED.
                                                 ---------------
                                                 Adrenal Cortex
                                                 Adrenal Medulla
                                                 Bone Marrow
                                                 Epididymis
                                                 Islets, Pancreatic
                                                 Kidney
                                                 Liver
                                                 Lung
                                                 Ovary
                                                 Pituitary Gland
                                                 Salivary Glands
                                                 Spleen
                                                 Testes
                                                 Thyroid Gland
                                                 Urinary Bladder
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08   
LAB: Battelle Columbus                                       ANTHRAQUINONE                                     DATE: 12/11/98     
EXPERIMENT: 88036 TEST: 04                                                                                     TIME: 09:00:07     
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                      
CONT: NO1-ES-25337                              ROUTE: DOSED FEED                                              NTP C#: C88036     
PATHOLOGIST: RYAN, M.                                                                                          CAS: 84-65-1       



SUMMARY OF STATISTICALLY SIGNIFICANT (P<=.05) RESULTS
IN THE ANALYSIS OF                  ANTHRAQUINONE
===============================================================

Male Mice
------------
Organ                                 Morphology
-----                                 ----------
Liver                                  Hepatoblastoma
                                       Hepatocellular Adenoma
                                       Hepatocellular Carcinoma
                                       Hepatocellular Carcinoma or Hepatoblastoma
                                       Hepatocellular Carcinoma or Hepatocellular Adenoma
                                       Hepatocellular Carcinoma, Hepatocellular Adenoma, or Hepatoblastoma
Thyroid Gland: Follicular Cell         Adenoma
                                       Carcinoma or Adenoma
All Organs                             Benign Tumors
                                       Malignant Tumors
                                       Malignant and Benign Tumors
===============================================================
Female Mice
------------
Organ                                  Morphology
-----                                  ----------
Liver                                  Hepatocellular Adenoma
                                       Hepatocellular Carcinoma
                                       Hepatocellular Carcinoma or Hepatoblastoma
                                       Hepatocellular Carcinoma or Hepatocellular Adenoma
                                       Hepatocellular Carcinoma, Hepatocellular Adenoma, or Hepatoblastoma
Thyroid Gland: Follicular Cell         Carcinoma
All Organs                             Benign Tumors
                                       Malignant and Benign Tumors
===============================================================
       Date:  12/11/98                           EXPERIMENT: 88036 TEST: 04                                            Page 1
                     Statistical Analysis of Primary Tumors in Mice(B6C3F1)        -                   ANTHRAQUINONE
                                         Terminal Sacrifice at 105 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Harderian Gland                                                                                                                  |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     3/50 (6%)    4/50 (8%)    4/50 (8%)    4/50 (8%)   |2/50 (4%)    3/50 (6%)    4/50 (8%)    1/50 (2%)    |
|POLY-3 ADJUSTED (b)|     6.1%         8.5%         8.4%         9.9%        |4.5%         6.4%         8.9%         2.2%         |
|TERMINAL (d)       |     1/45 (2%)    3/41 (7%)    3/43 (7%)    2/23 (9%)   |2/35 (6%)    3/42 (7%)    3/35 (9%)    1/42 (2%)    |
|FIRST INCIDENCE    |     550          662          662          647         |730 (T)      730 (T)      675          730 (T)      |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.199      P=0.460      P=0.480      P=0.264     |P=0.262N     P=0.583      P=0.338      P=0.436N     |
|POLY 3             |     P=0.375      P=0.480      P=0.486      P=0.398     |P=0.299N     P=0.518      P=0.339      P=0.491N     |
|POLY 1.5           |     P=0.429      P=0.492      P=0.495      P=0.447     |P=0.307N     P=0.512      P=0.341      P=0.498N     |
|POLY 6             |     P=0.307      P=0.465      P=0.479      P=0.334     |P=0.286N     P=0.527      P=0.335      P=0.481N     |
|LOGISTIC REGRESSION|     P=0.517      P=0.488      P=0.486      P=0.531     |P=0.286N     P=0.583      P=0.328      P=0.436N     |
|COCH-ARM / FISHERS |     P=0.492      P=0.500      P=0.500      P=0.500     |P=0.305N     P=0.500      P=0.339      P=0.500N     |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Harderian Gland                                                                                                                  |
|     Carcinoma or Adenoma                                                                                                        |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     3/50 (6%)    4/50 (8%)    4/50 (8%)    4/50 (8%)   |2/50 (4%)    4/50 (8%)    4/50 (8%)    1/50 (2%)    |
|POLY-3 ADJUSTED (b)|     6.1%         8.5%         8.4%         9.9%        |4.5%         8.6%         8.9%         2.2%         |
|TERMINAL (d)       |     1/45 (2%)    3/41 (7%)    3/43 (7%)    2/23 (9%)   |2/35 (6%)    4/42 (10%)   3/35 (9%)    1/42 (2%)    |
|FIRST INCIDENCE    |     550          662          662          647         |730 (T)      730 (T)      675          730 (T)      |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.199      P=0.460      P=0.480      P=0.264     |P=0.207N     P=0.424      P=0.338      P=0.436N     |
|POLY 3             |     P=0.375      P=0.480      P=0.486      P=0.398     |P=0.238N     P=0.356      P=0.339      P=0.491N     |
|POLY 1.5           |     P=0.429      P=0.492      P=0.495      P=0.447     |P=0.247N     P=0.350      P=0.341      P=0.498N     |
|POLY 6             |     P=0.307      P=0.465      P=0.479      P=0.334     |P=0.226N     P=0.365      P=0.335      P=0.481N     |
|LOGISTIC REGRESSION|     P=0.517      P=0.488      P=0.486      P=0.531     |P=0.227N     P=0.424      P=0.328      P=0.436N     |
|COCH-ARM / FISHERS |     P=0.492      P=0.500      P=0.500      P=0.500     |P=0.246N     P=0.339      P=0.339      P=0.500N     |
|=================================================================================================================================|
       Date:  12/11/98                           EXPERIMENT: 88036 TEST: 04                                            Page 2
                     Statistical Analysis of Primary Tumors in Mice(B6C3F1)       -                   ANTHRAQUINONE
                                         Terminal Sacrifice at 105 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Islets, Pancreatic                                                                                                               |
|     Carcinoma or Adenoma                                                                                                        |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     2/50 (4%)    0/50 (0%)    1/50 (2%)    0/42 (0%)   |0/50 (0%)    0/50 (0%)    0/50 (0%)    0/49 (0%)    |
|POLY-3 ADJUSTED (b)|     4.2%         0.0%         2.1%         0.0%        |0.0%         0.0%         0.0%         0.0%         |
|TERMINAL (d)       |     2/45 (4%)    0/41 (0%)    0/43 (0%)    0/23 (0%)   |0/35 (0%)    0/42 (0%)    0/35 (0%)    0/42 (0%)    |
|FIRST INCIDENCE    |     729 (T)      ---          727          ---         |---          ---          ---          ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.390N     P=0.259N     P=0.513N     P=0.395N    |(e)          (e)          (e)          (e)          |
|POLY 3             |     P=0.313N     P=0.243N     P=0.505N     P=0.306N    |(e)          (e)          (e)          (e)          |
|POLY 1.5           |     P=0.307N     P=0.239N     P=0.501N     P=0.291N    |(e)          (e)          (e)          (e)          |
|POLY 6             |     P=0.322N     P=0.248N     P=0.508N     P=0.330N    |(e)          (e)          (e)          (e)          |
|LOGISTIC REGRESSION|     P=0.379N     (e)          P=0.509N     (e)         |(e)          (e)          (e)          (e)          |
|COCH-ARM / FISHERS |     P=0.300N     P=0.247N     P=0.500N     P=0.293N    |(e)          (e)          (e)          (e)          |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney: Renal Tubule                                                                                                             |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     0/50 (0%)    1/50 (2%)    2/50 (4%)    0/47 (0%)   |0/49 (0%)    0/50 (0%)    0/49 (0%)    0/49 (0%)    |
|POLY-3 ADJUSTED (b)|     0.0%         2.1%         4.2%         0.0%        |0.0%         0.0%         0.0%         0.0%         |
|TERMINAL (d)       |     0/45 (0%)    1/41 (2%)    2/43 (5%)    0/23 (0%)   |0/35 (0%)    0/42 (0%)    0/35 (0%)    0/42 (0%)    |
|FIRST INCIDENCE    |     ---          729 (T)      729 (T)      ---         |---          ---          ---          ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.691N     P=0.481      P=0.229      (e)         |(e)          (e)          (e)          (e)          |
|POLY 3             |     P=0.600N     P=0.495      P=0.234      (e)         |(e)          (e)          (e)          (e)          |
|POLY 1.5           |     P=0.568N     P=0.498      P=0.237      (e)         |(e)          (e)          (e)          (e)          |
|POLY 6             |     P=0.643N     P=0.490      P=0.232      (e)         |(e)          (e)          (e)          (e)          |
|LOGISTIC REGRESSION|     (e)          P=0.481      P=0.229      (e)         |(e)          (e)          (e)          (e)          |
|COCH-ARM / FISHERS |     P=0.535N     P=0.500      P=0.247      (e)         |(e)          (e)          (e)          (e)          |
|=================================================================================================================================|
       Date:  12/11/98                           EXPERIMENT: 88036 TEST: 04                                            Page 3
                     Statistical Analysis of Primary Tumors in Mice(B6C3F1)       -                   ANTHRAQUINONE
                                         Terminal Sacrifice at 105 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatoblastoma                                                                                                              |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     1/50 (2%)    6/50 (12%)   11/50 (22%)  37/49 (76%) |0/49 (0%)    0/50 (0%)    0/50 (0%)    1/49 (2%)    |
|POLY-3 ADJUSTED (b)|     2.1%         12.8%        22.9%        79.2%       |0.0%         0.0%         0.0%         2.2%         |
|TERMINAL (d)       |     1/45 (2%)    5/41 (12%)   10/43 (23%)  15/23 (65%) |0/35 (0%)    0/42 (0%)    0/35 (0%)    1/42 (2%)    |
|FIRST INCIDENCE    |     729 (T)      658          481          490         |---          ---          ---          730 (T)      |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P<0.001 **   P=0.048 *    P=0.002 **   P<0.001 **  |P=0.234      (e)          (e)          P=0.536      |
|POLY 3             |     P<0.001 **   P=0.053      P=0.002 **   P<0.001 **  |P=0.214      (e)          (e)          P=0.504      |
|POLY 1.5           |     P<0.001 **   P=0.056      P=0.002 **   P<0.001 **  |P=0.212      (e)          (e)          P=0.501      |
|POLY 6             |     P<0.001 **   P=0.050      P=0.002 **   P<0.001 **  |P=0.217      (e)          (e)          P=0.510      |
|LOGISTIC REGRESSION|     P<0.001 **   P=0.056      P=0.003 **   P<0.001 **  |(e)          (e)          (e)          P=0.536      |
|COCH-ARM / FISHERS |     P<0.001 **   P=0.056      P=0.002 **   P<0.001 **  |P=0.213      (e)          (e)          P=0.500      |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Adenoma                                                                                                      |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     21/50 (42%)  32/50 (64%)  38/50 (76%)  41/49 (84%) |6/49 (12%)   28/50 (56%)  27/50 (54%)  40/49 (82%)  |
|POLY-3 ADJUSTED (b)|     43.4%        68.0%        79.7%        89.4%       |13.4%        59.5%        58.9%        87.2%        |
|TERMINAL (d)       |     19/45 (42%)  30/41 (73%)  36/43 (84%)  22/23 (96%) |4/35 (11%)   27/42 (64%)  23/35 (66%)  38/42 (91%)  |
|FIRST INCIDENCE    |     662          677          662          490         |519          611          626          549          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P<0.001 **   P=0.007 **   P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|POLY 3             |     P<0.001 **   P=0.011 *    P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|POLY 1.5           |     P<0.001 **   P=0.016 *    P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|POLY 6             |     P<0.001 **   P=0.007 **   P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|LOGISTIC REGRESSION|     P<0.001 **   P=0.008 **   P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|COCH-ARM / FISHERS |     P<0.001 **   P=0.022 *    P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|=================================================================================================================================|
       Date:  12/11/98                           EXPERIMENT: 88036 TEST: 04                                            Page 4
                     Statistical Analysis of Primary Tumors in Mice(B6C3F1)       -                   ANTHRAQUINONE
                                         Terminal Sacrifice at 105 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma                                                                                                    |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     8/50 (16%)   13/50 (26%)  17/50 (34%)  21/49 (43%) |2/49 (4%)    3/50 (6%)    8/50 (16%)   8/49 (16%)   |
|POLY-3 ADJUSTED (b)|     16.6%        27.1%        35.9%        49.8%       |4.5%         6.4%         17.4%        17.7%        |
|TERMINAL (d)       |     7/45 (16%)   11/41 (27%)  17/43 (40%)  14/23 (61%) |1/35 (3%)    3/42 (7%)    4/35 (11%)   7/42 (17%)   |
|FIRST INCIDENCE    |     702          464          729 (T)      456         |715          730 (T)      568          711          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P<0.001 **   P=0.120      P=0.024 *    P<0.001 **  |P=0.057      P=0.572      P=0.052      P=0.084      |
|POLY 3             |     P<0.001 **   P=0.160      P=0.026 *    P<0.001 **  |P=0.031 *    P=0.524      P=0.051      P=0.048 *    |
|POLY 1.5           |     P<0.001 **   P=0.167      P=0.029 *    P<0.001 **  |P=0.029 *    P=0.519      P=0.051      P=0.045 *    |
|POLY 6             |     P<0.001 **   P=0.150      P=0.024 *    P<0.001 **  |P=0.035 *    P=0.530      P=0.051      P=0.053      |
|LOGISTIC REGRESSION|     P<0.001 **   P=0.163      P=0.026 *    P<0.001 **  |P=0.031 *    P=0.541      P=0.051      P=0.058      |
|COCH-ARM / FISHERS |     P=0.004 **   P=0.163      P=0.032 *    P=0.003 **  |P=0.031 *    P=0.510      P=0.049 *    P=0.046 *    |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma or Hepatoblastoma                                                                                  |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     9/50 (18%)   18/50 (36%)  27/50 (54%)  45/49 (92%) |2/49 (4%)    3/50 (6%)    8/50 (16%)   8/49 (16%)   |
|POLY-3 ADJUSTED (b)|     18.7%        37.3%        56.2%        92.7%       |4.5%         6.4%         17.4%        17.7%        |
|TERMINAL (d)       |     8/45 (18%)   15/41 (37%)  26/43 (61%)  20/23 (87%) |1/35 (3%)    3/42 (7%)    4/35 (11%)   7/42 (17%)   |
|FIRST INCIDENCE    |     702          464          481          456         |715          730 (T)      568          711          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P<0.001 **   P=0.023 *    P<0.001 **   P<0.001 **  |P=0.057      P=0.572      P=0.052      P=0.084      |
|POLY 3             |     P<0.001 **   P=0.033 *    P<0.001 **   P<0.001 **  |P=0.031 *    P=0.524      P=0.051      P=0.048 *    |
|POLY 1.5           |     P<0.001 **   P=0.036 *    P<0.001 **   P<0.001 **  |P=0.029 *    P=0.519      P=0.051      P=0.045 *    |
|POLY 6             |     P<0.001 **   P=0.031 *    P<0.001 **   P<0.001 **  |P=0.035 *    P=0.530      P=0.051      P=0.053      |
|LOGISTIC REGRESSION|     P<0.001 **   P=0.036 *    P<0.001 **   P<0.001 **  |P=0.031 *    P=0.541      P=0.051      P=0.058      |
|COCH-ARM / FISHERS |     P<0.001 **   P=0.035 *    P<0.001 **   P<0.001 **  |P=0.031 *    P=0.510      P=0.049 *    P=0.046 *    |
|=================================================================================================================================|
       Date:  12/11/98                           EXPERIMENT: 88036 TEST: 04                                            Page 5
                     Statistical Analysis of Primary Tumors in Mice(B6C3F1)       -                   ANTHRAQUINONE
                                         Terminal Sacrifice at 105 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma or Hepatocellular Adenoma                                                                          |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     25/50 (50%)  34/50 (68%)  41/50 (82%)  46/49 (94%) |6/49 (12%)   30/50 (60%)  30/50 (60%)  41/49 (84%)  |
|POLY-3 ADJUSTED (b)|     51.7%        70.5%        86.0%        96.1%       |13.4%        63.8%        64.2%        89.3%        |
|TERMINAL (d)       |     23/45 (51%)  30/41 (73%)  39/43 (91%)  23/23 (100%)|4/35 (11%)   29/42 (69%)  24/35 (69%)  38/42 (91%)  |
|FIRST INCIDENCE    |     662          464          662          456         |519          611          568          549          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P<0.001 **   P=0.019 *    P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|POLY 3             |     P<0.001 **   P=0.043 *    P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|POLY 1.5           |     P<0.001 **   P=0.051      P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|POLY 6             |     P<0.001 **   P=0.034 *    P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|LOGISTIC REGRESSION|     P<0.001 **   P=0.043 *    P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|COCH-ARM / FISHERS |     P<0.001 **   P=0.052      P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma, Hepatocellular Adenoma,                                                                           |
|     or Hepatoblastoma                                                                                                           |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     26/50 (52%)  35/50 (70%)  43/50 (86%)  48/49 (98%) |6/49 (12%)   30/50 (60%)  30/50 (60%)  41/49 (84%)  |
|POLY-3 ADJUSTED (b)|     53.8%        72.2%        88.9%        98.9%       |13.4%        63.8%        64.2%        89.3%        |
|TERMINAL (d)       |     24/45 (53%)  30/41 (73%)  40/43 (93%)  23/23 (100%)|4/35 (11%)   29/42 (69%)  24/35 (69%)  38/42 (91%)  |
|FIRST INCIDENCE    |     662          464          481          456         |519          611          568          549          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P<0.001 **   P=0.020 *    P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|POLY 3             |     P<0.001 **   P=0.045 *    P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|POLY 1.5           |     P<0.001 **   P=0.051      P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|POLY 6             |     P<0.001 **   P=0.038 *    P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|LOGISTIC REGRESSION|     P<0.001 **   P=0.044 *    P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|COCH-ARM / FISHERS |     P<0.001 **   P=0.050      P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|=================================================================================================================================|
       Date:  12/11/98                           EXPERIMENT: 88036 TEST: 04                                            Page 6
                     Statistical Analysis of Primary Tumors in Mice(B6C3F1)       -                   ANTHRAQUINONE
                                         Terminal Sacrifice at 105 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Adenoma                                                                                                |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     11/50 (22%)  8/50 (16%)   6/50 (12%)   8/50 (16%)  |1/50 (2%)    1/50 (2%)    2/50 (4%)    3/49 (6%)    |
|POLY-3 ADJUSTED (b)|     22.7%        17.1%        12.7%        19.5%       |2.2%         2.1%         4.5%         6.7%         |
|TERMINAL (d)       |     8/45 (18%)   8/41 (20%)   6/43 (14%)   5/23 (22%)  |1/35 (3%)    1/42 (2%)    2/35 (6%)    3/42 (7%)    |
|FIRST INCIDENCE    |     662          729 (T)      729 (T)      490         |730 (T)      730 (T)      730 (T)      730 (T)      |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.241      P=0.390N     P=0.173N     P=0.362     |P=0.221      P=0.721N     P=0.500      P=0.372      |
|POLY 3             |     P=0.489N     P=0.335N     P=0.155N     P=0.454N    |P=0.179      P=0.752N     P=0.500      P=0.308      |
|POLY 1.5           |     P=0.431N     P=0.316N     P=0.146N     P=0.377N    |P=0.174      P=0.755N     P=0.502      P=0.300      |
|POLY 6             |     P=0.553      P=0.362N     P=0.163N     P=0.567N    |P=0.187      P=0.747N     P=0.496      P=0.320      |
|LOGISTIC REGRESSION|     P=0.513N     P=0.333N     P=0.148N     P=0.382N    |P=0.221      P=0.721N     P=0.500      P=0.372      |
|COCH-ARM / FISHERS |     P=0.367N     P=0.306N     P=0.143N     P=0.306N    |P=0.175      P=0.753N     P=0.500      P=0.301      |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Carcinoma                                                                                              |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     8/50 (16%)   12/50 (24%)  11/50 (22%)  4/50 (8%)   |3/50 (6%)    0/50 (0%)    0/50 (0%)    3/49 (6%)    |
|POLY-3 ADJUSTED (b)|     16.7%        25.4%        23.2%        10.1%       |6.6%         0.0%         0.0%         6.7%         |
|TERMINAL (d)       |     8/45 (18%)   11/41 (27%)  9/43 (21%)   4/23 (17%)  |2/35 (6%)    0/42 (0%)    0/35 (0%)    3/42 (7%)    |
|FIRST INCIDENCE    |     729 (T)      601          711          729 (T)     |613          ---          ---          730 (T)      |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.417N     P=0.165      P=0.274      P=0.616N    |P=0.335      P=0.102N     P=0.123N     P=0.589N     |
|POLY 3             |     P=0.138N     P=0.213      P=0.295      P=0.281N    |P=0.284      P=0.113N     P=0.120N     P=0.661      |
|POLY 1.5           |     P=0.092N     P=0.224      P=0.304      P=0.227N    |P=0.282      P=0.115N     P=0.119N     P=0.653      |
|POLY 6             |     P=0.225N     P=0.200      P=0.288      P=0.368N    |P=0.287      P=0.111N     P=0.123N     P=0.651N     |
|LOGISTIC REGRESSION|     P=0.256N     P=0.202      P=0.281      P=0.616N    |P=0.289      P=0.124N     P=0.121N     P=0.659      |
|COCH-ARM / FISHERS |     P=0.056N     P=0.227      P=0.306      P=0.178N    |P=0.289      P=0.121N     P=0.121N     P=0.651      |
|=================================================================================================================================|
       Date:  12/11/98                           EXPERIMENT: 88036 TEST: 04                                            Page 7
                     Statistical Analysis of Primary Tumors in Mice(B6C3F1)       -                   ANTHRAQUINONE
                                         Terminal Sacrifice at 105 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar                                                                      |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     17/50 (34%)  19/50 (38%)  17/50 (34%)  12/50 (24%) |4/50 (8%)    1/50 (2%)    2/50 (4%)    6/49 (12%)   |
|POLY-3 ADJUSTED (b)|     35.1%        40.2%        35.9%        29.2%       |8.8%         2.1%         4.5%         13.3%        |
|TERMINAL (d)       |     14/45 (31%)  18/41 (44%)  15/43 (35%)  9/23 (39%)  |3/35 (9%)    1/42 (2%)    2/35 (6%)    6/42 (14%)   |
|FIRST INCIDENCE    |     662          601          711          490         |613          730 (T)      730 (T)      730 (T)      |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.285      P=0.297      P=0.523      P=0.277     |P=0.133      P=0.141N     P=0.338N     P=0.477      |
|POLY 3             |     P=0.235N     P=0.379      P=0.554      P=0.356N    |P=0.089      P=0.169N     P=0.341N     P=0.368      |
|POLY 1.5           |     P=0.157N     P=0.407      P=0.575      P=0.264N    |P=0.087      P=0.172N     P=0.337N     P=0.358      |
|POLY 6             |     P=0.375N     P=0.344      P=0.533      P=0.505N    |P=0.094      P=0.165N     P=0.350N     P=0.385      |
|LOGISTIC REGRESSION|     P=0.343N     P=0.376      P=0.558      P=0.417N    |P=0.100      P=0.175N     P=0.337N     P=0.375      |
|COCH-ARM / FISHERS |     P=0.095N     P=0.418      P=0.583N     P=0.189N    |P=0.092      P=0.181N     P=0.339N     P=0.357      |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Ovary                                                                                                                            |
|     Cystadenoma                                                                                                                 |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |                                                        |2/45 (4%)    0/50 (0%)    2/49 (4%)    0/49 (0%)    |
|POLY-3 ADJUSTED (b)|                                                        |5.0%         0.0%         4.6%         0.0%         |
|TERMINAL (d)       |                                                        |2/33 (6%)    0/42 (0%)    2/34 (6%)    0/42 (0%)    |
|FIRST INCIDENCE    |                                                        |730 (T)      ---          730 (T)      ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |                                                        |P=0.248N     P=0.187N     P=0.685N     P=0.187N     |
|POLY 3             |                                                        |P=0.273N     P=0.205N     P=0.664N     P=0.212N     |
|POLY 1.5           |                                                        |P=0.275N     P=0.208N     P=0.661N     P=0.217N     |
|POLY 6             |                                                        |P=0.268N     P=0.202N     P=0.671N     P=0.206N     |
|LOGISTIC REGRESSION|                                                        |P=0.248N     (e)          P=0.685N     (e)          |
|COCH-ARM / FISHERS |                                                        |P=0.271N     P=0.222N     P=0.659N     P=0.226N     |
|=================================================================================================================================|
       Date:  12/11/98                           EXPERIMENT: 88036 TEST: 04                                            Page 8
                     Statistical Analysis of Primary Tumors in Mice(B6C3F1)       -                   ANTHRAQUINONE
                                         Terminal Sacrifice at 105 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Pituitary Gland: Pars Distalis or Unspecified Site                                                                               |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     0/48 (0%)    0/48 (0%)    1/47 (2%)    0/46 (0%)   |4/47 (9%)    7/50 (14%)   4/48 (8%)    6/46 (13%)   |
|POLY-3 ADJUSTED (b)|     0.0%         0.0%         2.2%         0.0%        |9.4%         15.0%        9.2%         14.1%        |
|TERMINAL (d)       |     0/43 (0%)    0/39 (0%)    1/41 (2%)    0/22 (0%)   |3/33 (9%)    6/42 (14%)   3/35 (9%)    5/39 (13%)   |
|FIRST INCIDENCE    |     ---          ---          729 (T)      ---         |717          703          626          549          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.797      (e)          P=0.490      (e)         |P=0.500      P=0.397      P=0.622N     P=0.461      |
|POLY 3             |     P=0.770      (e)          P=0.493      (e)         |P=0.428      P=0.318      P=0.632N     P=0.372      |
|POLY 1.5           |     P=0.796      (e)          P=0.496      (e)         |P=0.415      P=0.311      P=0.629N     P=0.359      |
|POLY 6             |     P=0.727      (e)          P=0.492      (e)         |P=0.451      P=0.328      P=0.637N     P=0.393      |
|LOGISTIC REGRESSION|     (e)          (e)          P=0.490      (e)         |P=0.415      P=0.340      P=0.639N     P=0.356      |
|COCH-ARM / FISHERS |     P=0.792N     (e)          P=0.495      (e)         |P=0.416      P=0.299      P=0.631N     P=0.356      |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin                                                                                                                             |
|     Fibroma, Fibrosarcoma, Sarcoma, Myxoma, Myxosarcoma,                                                                        |
|     or Fibrous Histiocytoma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     0/50 (0%)    0/50 (0%)    0/50 (0%)    0/50 (0%)   |2/50 (4%)    3/50 (6%)    2/50 (4%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     0.0%         0.0%         0.0%         0.0%        |4.4%         6.4%         4.4%         0.0%         |
|TERMINAL (d)       |     0/45 (0%)    0/41 (0%)    0/43 (0%)    0/23 (0%)   |0/35 (0%)    2/42 (5%)    1/35 (3%)    0/42 (0%)    |
|FIRST INCIDENCE    |     ---          ---          ---          ---         |617          648          627          ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     (e)          (e)          (e)          (e)         |P=0.110N     P=0.550      P=0.686      P=0.224N     |
|POLY 3             |     (e)          (e)          (e)          (e)         |P=0.117N     P=0.517      P=0.693      P=0.234N     |
|POLY 1.5           |     (e)          (e)          (e)          (e)         |P=0.120N     P=0.511      P=0.692N     P=0.237N     |
|POLY 6             |     (e)          (e)          (e)          (e)         |P=0.113N     P=0.524      P=0.689      P=0.229N     |
|LOGISTIC REGRESSION|     (e)          (e)          (e)          (e)         |P=0.113N     P=0.482      P=0.683      P=0.233N     |
|COCH-ARM / FISHERS |     (e)          (e)          (e)          (e)         |P=0.118N     P=0.500      P=0.691N     P=0.247N     |
|=================================================================================================================================|
       Date:  12/11/98                           EXPERIMENT: 88036 TEST: 04                                            Page 9
                     Statistical Analysis of Primary Tumors in Mice(B6C3F1)       -                   ANTHRAQUINONE
                                         Terminal Sacrifice at 105 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin                                                                                                                             |
|     Fibrosarcoma                                                                                                                |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     0/50 (0%)    0/50 (0%)    0/50 (0%)    0/50 (0%)   |2/50 (4%)    2/50 (4%)    1/50 (2%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     0.0%         0.0%         0.0%         0.0%        |4.4%         4.3%         2.2%         0.0%         |
|TERMINAL (d)       |     0/45 (0%)    0/41 (0%)    0/43 (0%)    0/23 (0%)   |0/35 (0%)    2/42 (5%)    0/35 (0%)    0/42 (0%)    |
|FIRST INCIDENCE    |     ---          ---          ---          ---         |617          730 (T)      627          ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     (e)          (e)          (e)          (e)         |P=0.135N     P=0.648N     P=0.507N     P=0.224N     |
|POLY 3             |     (e)          (e)          (e)          (e)         |P=0.142N     P=0.683N     P=0.501N     P=0.234N     |
|POLY 1.5           |     (e)          (e)          (e)          (e)         |P=0.144N     P=0.686N     P=0.499N     P=0.237N     |
|POLY 6             |     (e)          (e)          (e)          (e)         |P=0.139N     P=0.679N     P=0.505N     P=0.229N     |
|LOGISTIC REGRESSION|     (e)          (e)          (e)          (e)         |P=0.137N     P=0.693      P=0.529N     P=0.233N     |
|COCH-ARM / FISHERS |     (e)          (e)          (e)          (e)         |P=0.142N     P=0.691N     P=0.500N     P=0.247N     |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin                                                                                                                             |
|     Fibrosarcoma, Sarcoma, Myxosarcoma, or Fibrous Histiocytoma                                                                 |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     0/50 (0%)    0/50 (0%)    0/50 (0%)    0/50 (0%)   |2/50 (4%)    3/50 (6%)    2/50 (4%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     0.0%         0.0%         0.0%         0.0%        |4.4%         6.4%         4.4%         0.0%         |
|TERMINAL (d)       |     0/45 (0%)    0/41 (0%)    0/43 (0%)    0/23 (0%)   |0/35 (0%)    2/42 (5%)    1/35 (3%)    0/42 (0%)    |
|FIRST INCIDENCE    |     ---          ---          ---          ---         |617          648          627          ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     (e)          (e)          (e)          (e)         |P=0.110N     P=0.550      P=0.686      P=0.224N     |
|POLY 3             |     (e)          (e)          (e)          (e)         |P=0.117N     P=0.517      P=0.693      P=0.234N     |
|POLY 1.5           |     (e)          (e)          (e)          (e)         |P=0.120N     P=0.511      P=0.692N     P=0.237N     |
|POLY 6             |     (e)          (e)          (e)          (e)         |P=0.113N     P=0.524      P=0.689      P=0.229N     |
|LOGISTIC REGRESSION|     (e)          (e)          (e)          (e)         |P=0.113N     P=0.482      P=0.683      P=0.233N     |
|COCH-ARM / FISHERS |     (e)          (e)          (e)          (e)         |P=0.118N     P=0.500      P=0.691N     P=0.247N     |
|=================================================================================================================================|
       Date:  12/11/98                           EXPERIMENT: 88036 TEST: 04                                            Page 10
                     Statistical Analysis of Primary Tumors in Mice(B6C3F1)       -                   ANTHRAQUINONE
                                         Terminal Sacrifice at 105 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin                                                                                                                             |
|     Schwannoma Malignant                                                                                                        |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     0/50 (0%)    0/50 (0%)    2/50 (4%)    0/50 (0%)   |0/50 (0%)    0/50 (0%)    0/50 (0%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     0.0%         0.0%         4.2%         0.0%        |0.0%         0.0%         0.0%         0.0%         |
|TERMINAL (d)       |     0/45 (0%)    0/41 (0%)    1/43 (2%)    0/23 (0%)   |0/35 (0%)    0/42 (0%)    0/35 (0%)    0/42 (0%)    |
|FIRST INCIDENCE    |     ---          ---          453          ---         |---          ---          ---          ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.725      (e)          P=0.237      (e)         |(e)          (e)          (e)          (e)          |
|POLY 3             |     P=0.684      (e)          P=0.238      (e)         |(e)          (e)          (e)          (e)          |
|POLY 1.5           |     P=0.724      (e)          P=0.239      (e)         |(e)          (e)          (e)          (e)          |
|POLY 6             |     P=0.616      (e)          P=0.237      (e)         |(e)          (e)          (e)          (e)          |
|LOGISTIC REGRESSION|     P=0.688N     (e)          P=0.178      (e)         |(e)          (e)          (e)          (e)          |
|COCH-ARM / FISHERS |     P=0.695N     (e)          P=0.247      (e)         |(e)          (e)          (e)          (e)          |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Spleen                                                                                                                           |
|     Hemangiosarcoma                                                                                                             |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     2/50 (4%)    0/50 (0%)    0/49 (0%)    0/42 (0%)   |0/45 (0%)    1/49 (2%)    0/48 (0%)    0/48 (0%)    |
|POLY-3 ADJUSTED (b)|     4.2%         0.0%         0.0%         0.0%        |0.0%         2.2%         0.0%         0.0%         |
|TERMINAL (d)       |     1/45 (2%)    0/41 (0%)    0/43 (0%)    0/23 (0%)   |0/35 (0%)    0/42 (0%)    0/35 (0%)    0/42 (0%)    |
|FIRST INCIDENCE    |     710          ---          ---          ---         |---          611          ---          ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.322N     P=0.260N     P=0.247N     P=0.399N    |P=0.579N     P=0.513      (e)          (e)          |
|POLY 3             |     P=0.255N     P=0.244N     P=0.242N     P=0.310N    |P=0.577N     P=0.522      (e)          (e)          |
|POLY 1.5           |     P=0.259N     P=0.239N     P=0.241N     P=0.293N    |P=0.579N     P=0.521      (e)          (e)          |
|POLY 6             |     P=0.248N     P=0.249N     P=0.244N     P=0.335N    |P=0.574N     P=0.524      (e)          (e)          |
|LOGISTIC REGRESSION|     P=0.276N     P=0.240N     P=0.241N     P=0.323N    |P=0.529N     P=0.459      (e)          (e)          |
|COCH-ARM / FISHERS |     P=0.262N     P=0.247N     P=0.253N     P=0.293N    |P=0.581N     P=0.521      (e)          (e)          |
|=================================================================================================================================|
       Date:  12/11/98                           EXPERIMENT: 88036 TEST: 04                                            Page 11
                     Statistical Analysis of Primary Tumors in Mice(B6C3F1)       -                   ANTHRAQUINONE
                                         Terminal Sacrifice at 105 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Stomach, Forestomach                                                                                                             |
|     Squamous Cell Papilloma                                                                                                     |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     0/50 (0%)    0/50 (0%)    0/50 (0%)    0/50 (0%)   |3/50 (6%)    0/50 (0%)    0/50 (0%)    1/50 (2%)    |
|POLY-3 ADJUSTED (b)|     0.0%         0.0%         0.0%         0.0%        |6.7%         0.0%         0.0%         2.2%         |
|TERMINAL (d)       |     0/45 (0%)    0/41 (0%)    0/43 (0%)    0/23 (0%)   |3/35 (9%)    0/42 (0%)    0/35 (0%)    1/42 (2%)    |
|FIRST INCIDENCE    |     ---          ---          ---          ---         |730 (T)      ---          ---          730 (T)      |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     (e)          (e)          (e)          (e)         |P=0.409N     P=0.091N     P=0.121N     P=0.242N     |
|POLY 3             |     (e)          (e)          (e)          (e)         |P=0.450N     P=0.112N     P=0.119N     P=0.297N     |
|POLY 1.5           |     (e)          (e)          (e)          (e)         |P=0.449N     P=0.114N     P=0.118N     P=0.304N     |
|POLY 6             |     (e)          (e)          (e)          (e)         |P=0.450N     P=0.108N     P=0.121N     P=0.286N     |
|LOGISTIC REGRESSION|     (e)          (e)          (e)          (e)         |P=0.409N     (e)          (e)          P=0.242N     |
|COCH-ARM / FISHERS |     (e)          (e)          (e)          (e)         |P=0.443N     P=0.121N     P=0.121N     P=0.309N     |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Thyroid Gland: Follicular Cell                                                                                                   |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     0/50 (0%)    0/50 (0%)    2/49 (4%)    2/46 (4%)   |1/45 (2%)    1/48 (2%)    2/48 (4%)    2/48 (4%)    |
|POLY-3 ADJUSTED (b)|     0.0%         0.0%         4.3%         5.3%        |2.4%         2.2%         4.6%         4.5%         |
|TERMINAL (d)       |     0/45 (0%)    0/41 (0%)    2/43 (5%)    2/23 (9%)   |1/35 (3%)    1/42 (2%)    2/35 (6%)    2/42 (5%)    |
|FIRST INCIDENCE    |     ---          ---          729 (T)      729 (T)     |730 (T)      730 (T)      730 (T)      730 (T)      |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.031 *    (e)          P=0.229      P=0.107     |P=0.429      P=0.721N     P=0.500      P=0.564      |
|POLY 3             |     P=0.078      (e)          P=0.230      P=0.188     |P=0.393      P=0.741N     P=0.519      P=0.522      |
|POLY 1.5           |     P=0.091      (e)          P=0.233      P=0.203     |P=0.390      P=0.742N     P=0.523      P=0.519      |
|POLY 6             |     P=0.063      (e)          P=0.229      P=0.168     |P=0.399      P=0.739N     P=0.513      P=0.528      |
|LOGISTIC REGRESSION|     (e)          (e)          P=0.229      P=0.107     |P=0.429      P=0.721N     P=0.500      P=0.564      |
|COCH-ARM / FISHERS |     P=0.107      (e)          P=0.242      P=0.227     |P=0.397      P=0.736N     P=0.524      P=0.524      |
|=================================================================================================================================|
       Date:  12/11/98                           EXPERIMENT: 88036 TEST: 04                                            Page 12
                     Statistical Analysis of Primary Tumors in Mice(B6C3F1)       -                   ANTHRAQUINONE
                                         Terminal Sacrifice at 105 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Thyroid Gland: Follicular Cell                                                                                                   |
|     Carcinoma                                                                                                                   |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     0/50 (0%)    0/50 (0%)    0/49 (0%)    0/46 (0%)   |0/45 (0%)    0/48 (0%)    0/48 (0%)    2/48 (4%)    |
|POLY-3 ADJUSTED (b)|     0.0%         0.0%         0.0%         0.0%        |0.0%         0.0%         0.0%         4.5%         |
|TERMINAL (d)       |     0/45 (0%)    0/41 (0%)    0/43 (0%)    0/23 (0%)   |0/35 (0%)    0/42 (0%)    0/35 (0%)    2/42 (5%)    |
|FIRST INCIDENCE    |     ---          ---          ---          ---         |---          ---          ---          730 (T)      |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     (e)          (e)          (e)          (e)         |P=0.052      (e)          (e)          P=0.279      |
|POLY 3             |     (e)          (e)          (e)          (e)         |P=0.042 *    (e)          (e)          P=0.252      |
|POLY 1.5           |     (e)          (e)          (e)          (e)         |P=0.042 *    (e)          (e)          P=0.250      |
|POLY 6             |     (e)          (e)          (e)          (e)         |P=0.043 *    (e)          (e)          P=0.255      |
|LOGISTIC REGRESSION|     (e)          (e)          (e)          (e)         |(e)          (e)          (e)          P=0.279      |
|COCH-ARM / FISHERS |     (e)          (e)          (e)          (e)         |P=0.044 *    (e)          (e)          P=0.264      |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Thyroid Gland: Follicular Cell                                                                                                   |
|     Carcinoma or Adenoma                                                                                                        |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |                                                        |                                                    |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     0/50 (0%)    0/50 (0%)    2/49 (4%)    2/46 (4%)   |1/45 (2%)    1/48 (2%)    2/48 (4%)    4/48 (8%)    |
|POLY-3 ADJUSTED (b)|     0.0%         0.0%         4.3%         5.3%        |2.4%         2.2%         4.6%         9.1%         |
|TERMINAL (d)       |     0/45 (0%)    0/41 (0%)    2/43 (5%)    2/23 (9%)   |1/35 (3%)    1/42 (2%)    2/35 (6%)    4/42 (10%)   |
|FIRST INCIDENCE    |     ---          ---          729 (T)      729 (T)     |730 (T)      730 (T)      730 (T)      730 (T)      |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.031 *    (e)          P=0.229      P=0.107     |P=0.099      P=0.721N     P=0.500      P=0.238      |
|POLY 3             |     P=0.078      (e)          P=0.230      P=0.188     |P=0.078      P=0.741N     P=0.519      P=0.198      |
|POLY 1.5           |     P=0.091      (e)          P=0.233      P=0.203     |P=0.077      P=0.742N     P=0.523      P=0.196      |
|POLY 6             |     P=0.063      (e)          P=0.229      P=0.168     |P=0.080      P=0.739N     P=0.513      P=0.204      |
|LOGISTIC REGRESSION|     (e)          (e)          P=0.229      P=0.107     |P=0.099      P=0.721N     P=0.500      P=0.238      |
|COCH-ARM / FISHERS |     P=0.107      (e)          P=0.242      P=0.227     |P=0.080      P=0.736N     P=0.524      P=0.201      |
|=================================================================================================================================|
       Date:  12/11/98                           EXPERIMENT: 88036 TEST: 04                                            Page 13
                     Statistical Analysis of Primary Tumors in Mice(B6C3F1)       -                   ANTHRAQUINONE
                                         Terminal Sacrifice at 105 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Hemangiosarcoma                                                                                                             |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     2/50 (4%)    0/50 (0%)    1/50 (2%)    3/50 (6%)   |1/50 (2%)    1/50 (2%)    0/50 (0%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     4.2%         0.0%         2.1%         7.5%        |2.2%         2.1%         0.0%         0.0%         |
|TERMINAL (d)       |     1/45 (2%)    0/41 (0%)    1/43 (2%)    2/23 (9%)   |0/35 (0%)    0/42 (0%)    0/35 (0%)    0/42 (0%)    |
|FIRST INCIDENCE    |     710          ---          729 (T)      647         |717          611          ---          ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.053      P=0.260N     P=0.513N     P=0.240     |P=0.301N     P=0.747N     P=0.516N     P=0.480N     |
|POLY 3             |     P=0.151      P=0.244N     P=0.505N     P=0.418     |P=0.305N     P=0.750N     P=0.500N     P=0.495N     |
|POLY 1.5           |     P=0.166      P=0.239N     P=0.501N     P=0.458     |P=0.307N     P=0.754N     P=0.499N     P=0.499N     |
|POLY 6             |     P=0.133      P=0.249N     P=0.509N     P=0.364     |P=0.303N     P=0.745N     P=0.503N     P=0.490N     |
|LOGISTIC REGRESSION|     P=0.134      P=0.240N     P=0.504N     P=0.407     |P=0.290N     P=0.742      P=0.502N     P=0.495N     |
|COCH-ARM / FISHERS |     P=0.187      P=0.247N     P=0.500N     P=0.500     |P=0.305N     P=0.753N     P=0.500N     P=0.500N     |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Hemangiosarcoma or Hemangioma                                                                                               |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     2/50 (4%)    0/50 (0%)    1/50 (2%)    3/50 (6%)   |1/50 (2%)    2/50 (4%)    1/50 (2%)    0/50 (0%)    |
|POLY-3 ADJUSTED (b)|     4.2%         0.0%         2.1%         7.5%        |2.2%         4.3%         2.2%         0.0%         |
|TERMINAL (d)       |     1/45 (2%)    0/41 (0%)    1/43 (2%)    2/23 (9%)   |0/35 (0%)    1/42 (2%)    1/35 (3%)    0/42 (0%)    |
|FIRST INCIDENCE    |     710          ---          729 (T)      647         |717          611          730 (T)      ---          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.053      P=0.260N     P=0.513N     P=0.240     |P=0.222N     P=0.535      P=0.751      P=0.480N     |
|POLY 3             |     P=0.151      P=0.244N     P=0.505N     P=0.418     |P=0.231N     P=0.517      P=0.760      P=0.495N     |
|POLY 1.5           |     P=0.166      P=0.239N     P=0.501N     P=0.458     |P=0.235N     P=0.511      P=0.760N     P=0.499N     |
|POLY 6             |     P=0.133      P=0.249N     P=0.509N     P=0.364     |P=0.225N     P=0.525      P=0.757      P=0.490N     |
|LOGISTIC REGRESSION|     P=0.134      P=0.240N     P=0.504N     P=0.407     |P=0.234N     P=0.484      P=0.758      P=0.495N     |
|COCH-ARM / FISHERS |     P=0.187      P=0.247N     P=0.500N     P=0.500     |P=0.235N     P=0.500      P=0.753N     P=0.500N     |
|=================================================================================================================================|
       Date:  12/11/98                           EXPERIMENT: 88036 TEST: 04                                            Page 14
                     Statistical Analysis of Primary Tumors in Mice(B6C3F1)       -                   ANTHRAQUINONE
                                         Terminal Sacrifice at 105 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Histiocytic Sarcoma                                                                                                         |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     0/50 (0%)    1/50 (2%)    2/50 (4%)    1/50 (2%)   |2/50 (4%)    1/50 (2%)    2/50 (4%)    1/50 (2%)    |
|POLY-3 ADJUSTED (b)|     0.0%         2.1%         4.2%         2.5%        |4.4%         2.1%         4.4%         2.2%         |
|TERMINAL (d)       |     0/45 (0%)    1/41 (2%)    1/43 (2%)    0/23 (0%)   |0/35 (0%)    0/42 (0%)    0/35 (0%)    1/42 (2%)    |
|FIRST INCIDENCE    |     ---          729 (T)      685          623         |554          549          491          730 (T)      |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.347      P=0.481      P=0.232      P=0.448     |P=0.470N     P=0.497N     P=0.686      P=0.479N     |
|POLY 3             |     P=0.416      P=0.495      P=0.235      P=0.465     |P=0.482N     P=0.489N     P=0.692      P=0.499N     |
|POLY 1.5           |     P=0.456      P=0.498      P=0.237      P=0.482     |P=0.484N     P=0.492N     P=0.692N     P=0.502N     |
|POLY 6             |     P=0.359      P=0.490      P=0.234      P=0.441     |P=0.477N     P=0.484N     P=0.687      P=0.492N     |
|LOGISTIC REGRESSION|     P=0.514      P=0.481      P=0.237      P=0.510     |P=0.371N     P=0.652N     P=0.615      P=0.483N     |
|COCH-ARM / FISHERS |     P=0.500      P=0.500      P=0.247      P=0.500     |P=0.476N     P=0.500N     P=0.691N     P=0.500N     |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Lymphoma: Histiocytic, Lymphocytic, Mixed,                                                                        |
|     NOS, or Undifferentiated Cell Type                                                                                          |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     3/50 (6%)    3/50 (6%)    2/50 (4%)    0/50 (0%)   |14/50 (28%)  8/50 (16%)   8/50 (16%)   10/50 (20%)  |
|POLY-3 ADJUSTED (b)|     6.3%         6.4%         4.2%         0.0%        |30.6%        16.7%        17.4%        21.6%        |
|TERMINAL (d)       |     3/45 (7%)    3/41 (7%)    2/43 (5%)    0/23 (0%)   |11/35 (31%)  6/42 (14%)   5/35 (14%)   9/42 (21%)   |
|FIRST INCIDENCE    |     729 (T)      729 (T)      729 (T)      ---         |519          499          505          625          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P=0.171N     P=0.619      P=0.521N     P=0.262N    |P=0.313N     P=0.060N     P=0.128N     P=0.130N     |
|POLY 3             |     P=0.104N     P=0.650      P=0.506N     P=0.156N    |P=0.413N     P=0.089N     P=0.107N     P=0.229N     |
|POLY 1.5           |     P=0.089N     P=0.658      P=0.501N     P=0.137N    |P=0.419N     P=0.097N     P=0.107N     P=0.242N     |
|POLY 6             |     P=0.127N     P=0.640      P=0.510N     P=0.187N    |P=0.398N     P=0.081N     P=0.110N     P=0.208N     |
|LOGISTIC REGRESSION|     P=0.171N     P=0.619      P=0.521N     (e)         |P=0.402N     P=0.117N     P=0.113N     P=0.229N     |
|COCH-ARM / FISHERS |     P=0.074N     P=0.661N     P=0.500N     P=0.121N    |P=0.400N     P=0.114N     P=0.114N     P=0.241N     |
|=================================================================================================================================|
       Date:  12/11/98                           EXPERIMENT: 88036 TEST: 04                                            Page 15
                     Statistical Analysis of Primary Tumors in Mice(B6C3F1)       -                   ANTHRAQUINONE
                                         Terminal Sacrifice at 105 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Benign Tumors                                                                                                               |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     27/50 (54%)  34/50 (68%)  41/50 (82%)  43/50 (86%) |13/50 (26%)  31/50 (62%)  32/50 (64%)  40/50 (80%)  |
|POLY-3 ADJUSTED (b)|     55.1%        71.9%        86.0%        91.7%       |28.5%        65.7%        69.5%        85.7%        |
|TERMINAL (d)       |     23/45 (51%)  31/41 (76%)  38/43 (88%)  22/23 (96%) |10/35 (29%)  29/42 (69%)  27/35 (77%)  38/42 (91%)  |
|FIRST INCIDENCE    |     550          662          662          490         |519          611          626          549          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P<0.001 **   P=0.046 *    P=0.002 **   P<0.001 **  |P<0.001 **   P=0.003 **   P<0.001 **   P<0.001 **   |
|POLY 3             |     P<0.001 **   P=0.064      P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|POLY 1.5           |     P<0.001 **   P=0.087      P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|POLY 6             |     P<0.001 **   P=0.043 *    P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|LOGISTIC REGRESSION|     P<0.001 **   P=0.078      P<0.001 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|COCH-ARM / FISHERS |     P<0.001 **   P=0.109      P=0.002 **   P<0.001 **  |P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 **   |
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Tumors                                                                                                            |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     18/50 (36%)  28/50 (56%)  33/50 (66%)  45/50 (90%) |21/50 (42%)  17/50 (34%)  18/50 (36%)  25/50 (50%)  |
|POLY-3 ADJUSTED (b)|     37.4%        57.8%        67.3%        91.9%       |44.1%        34.6%        37.6%        52.2%        |
|TERMINAL (d)       |     16/45 (36%)  24/41 (59%)  28/43 (65%)  20/23 (87%) |13/35 (37%)  12/42 (29%)  9/35 (26%)   21/42 (50%)  |
|FIRST INCIDENCE    |     702          464          453          456         |519          499          491          186          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P<0.001 **   P=0.018 *    P=0.003 **   P<0.001 **  |P=0.232      P=0.158N     P=0.374N     P=0.521      |
|POLY 3             |     P<0.001 **   P=0.033 *    P=0.002 **   P<0.001 **  |P=0.099      P=0.227N     P=0.327N     P=0.281      |
|POLY 1.5           |     P<0.001 **   P=0.036 *    P=0.002 **   P<0.001 **  |P=0.100      P=0.238N     P=0.325N     P=0.274      |
|POLY 6             |     P<0.001 **   P=0.029 *    P=0.002 **   P<0.001 **  |P=0.100      P=0.215N     P=0.339N     P=0.291      |
|LOGISTIC REGRESSION|     P<0.001 **   P=0.033 *    P=0.003 **   P<0.001 **  |P=0.116      P=0.315N     P=0.351N     P=0.274      |
|COCH-ARM / FISHERS |     P<0.001 **   P=0.035 *    P=0.002 **   P<0.001 **  |P=0.110      P=0.268N     P=0.341N     P=0.274      |
|=================================================================================================================================|
       Date:  12/11/98                           EXPERIMENT: 88036 TEST: 04                                            Page 16
                     Statistical Analysis of Primary Tumors in Mice(B6C3F1)       -                   ANTHRAQUINONE
                                         Terminal Sacrifice at 105 weeks 
|=================================================================================================================================|
|                   |                                                        |                                                    |
|                   |                              Males                     |                       Females                      |
|Dose               |     0 PPM        833 PPM      2500 PPM     7500 PPM    |0 PPM        833 PPM      2500 PPM     7500 PPM     |
|                   |                                                        |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant and Benign Tumors                                                                                                 |
|                                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES        |     #            #            #            #           |#            #            #            #            |
|-----------        |                                                        |                                                    |
|OVERALL (a)        |     35/50 (70%)  38/50 (76%)  47/50 (94%)  48/50 (96%) |28/50 (56%)  38/50 (76%)  41/50 (82%)  44/50 (88%)  |
|POLY-3 ADJUSTED (b)|     71.4%        78.0%        95.3%        98.0%       |58.8%        77.2%        83.9%        91.5%        |
|TERMINAL (d)       |     31/45 (69%)  32/41 (78%)  41/43 (95%)  23/23 (100%)|19/35 (54%)  32/42 (76%)  29/35 (83%)  39/42 (93%)  |
|FIRST INCIDENCE    |     550          464          453          456         |519          499          491          186          |
|-------------------|                                                        |                                                    |
|STATISTICAL TESTS  |                                                        |                                                    |
|-------------------|                                                        |                                                    |
|LIFE TABLE         |     P<0.001 **   P=0.157      P=0.004 **   P<0.001 **  |P=0.056      P=0.238      P=0.021 *    P=0.038 *    |
|POLY 3             |     P<0.001 **   P=0.305      P<0.001 **   P<0.001 **  |P<0.001 **   P=0.039 *    P=0.004 **   P<0.001 **   |
|POLY 1.5           |     P<0.001 **   P=0.336      P<0.001 **   P<0.001 **  |P<0.001 **   P=0.035 *    P=0.004 **   P<0.001 **   |
|POLY 6             |     P<0.001 **   P=0.269      P<0.001 **   P<0.001 **  |P<0.001 **   P=0.043 *    P=0.004 **   P<0.001 **   |
|LOGISTIC REGRESSION|     P<0.001 **   P=0.322      P=0.002 **   P<0.001 **  |P<0.001 **   P=0.032 *    P=0.005 **   P<0.001 **   |
|COCH-ARM / FISHERS |     P<0.001 **   P=0.326      P=0.002 **   P<0.001 **  |P<0.001 **   P=0.028 *    P=0.004 **   P<0.001 **   |
|=================================================================================================================================|

(a)  Number of tumor-bearing animals /  number of animals examined at site.
(b)  Poly-3 adjusted lifetime tumor incidence.
(d)  Observed incidence at terminal kill.
(f)  Beneath the control incidence are the P-values associated with the trend 
test.  Beneath the dosed group incidence are the P-values corresponding to 
pairwise comparisons between the controls and that dosed group.  The life
table analysis regards tumors in animals dying prior to  terminal kill as
 being (directly or indirectly) the cause of death.
Logistic regression is an alternative
 method for analyzing the incidence of non-fatal tumors. The Cochran-Armitage
and Fishers exact tests compare directly the overall incidence rates
For all tests a negative trend is indicated by N
(e)  Value of Statistic cannot be computed.
(I)  Interim sacrifice
(T)  Terminal sacrifice
#   Tumor rates based on number of animals necropsied.
*   To the right of any statistical result, indicates significance at (P<=0.05).
**  To the right of any statistical result, indicates significance at (P<=0.01).