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TDMS Study 99033-02 Pathology Tables

NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03
Route: DOSED FEED                                                                                                 Time: 11:36:07

                                                       FINAL #3/P53 MICE




       Facility:  BIORELIANCE

       Chemical CAS #:  22839-47-0

       Lock Date:  07/20/01

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All




































                                                              Page   1


NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 3| 7| 7|                             |            |
                                           | 8| 8| 8| 5| 8| 8| 5| 5| 8| 5| 5| 8| 6| 8| 8|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    VEHICLE                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1|                             |      L     |
    CONTROL                                | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Gallbladder                             | +  +  +  M  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |    1                 1        1  1                                       |      4  1.0|
      Pigmentation, Focal, Hemosiderin     |          3                                                               |      1  3.0|
      Tension Lipidosis                    |                      X                                                   |      1     |
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       | 1     1  1           1  1                 1                              |      6  1.0|
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Tongue                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page   2                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 3| 7| 7|                             |            |
                                           | 8| 8| 8| 5| 8| 8| 5| 5| 8| 5| 5| 8| 6| 8| 8|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    VEHICLE                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1|                             |      L     |
    CONTROL                                | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Blood Vessel                            | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Subcapsular, Hyperplasia, Focal      | 3  1  1     1  1     1  1  1  1  2     1  1                              |     12  1.3|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  M  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | M  M  M  M  +  M  M  +  M  +  +  M  M  +  +                              |   6        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | M  M  +  +  I  +  +  +  I  +  +  +  I  I  +                              |   9        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  M  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
      Ectopic Thymus                       |             1  1                                                         |      2  1.0|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Clitoral Gland                          | M  +  +  +  +  +  +  +  +  +  +  +  M  +  M                              |  12        |
                                           |__________________________________________________________________________|____________|
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Cyst                                 |                                        3                                 |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Endometrium, Hyperplasia, Cystic     | 1  1  2  2  2  1  1  2  1  1  1  2     1  2                              |     14  1.4|
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page   3                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 3| 7| 7|                             |            |
                                           | 8| 8| 8| 5| 8| 8| 5| 5| 8| 5| 5| 8| 6| 8| 8|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    VEHICLE                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1|                             |      L     |
    CONTROL                                | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM - cont                     |                                                                          |            |
   Vagina                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hyperplasia, Histiocytic             |                   2                                                      |      1  2.0|
      Hyperplasia, Lymphoid                |                               2     3                                    |      2  2.5|
      Hyperplasia                          |                2                                                         |      1  2.0|
      Hyperplasia, Plasma Cell             |                            1                                             |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hyperplasia, Lymphoid                |                               2                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | M  +  +  +  +  +  M  +  +  +  +  +  +  M  +                              |  12        |
      Hyperplasia, Lymphoid                |                               4                                          |      1  4.0|
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hematopoietic Cell Proliferation     | 2     1                             3                                    |      3  2.0|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | M  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
      Atrophy, Focal                       |                                  1                                       |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  M  M  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page   4                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 3| 7| 7|                             |            |
                                           | 8| 8| 8| 5| 8| 8| 5| 5| 8| 5| 5| 8| 6| 8| 8|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    VEHICLE                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1|                             |      L     |
    CONTROL                                | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 MUSCULOSKELETAL SYSTEM - cont             |                                                                          |            |
   Skeletal Muscle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Larynx                                  | M  M  M  +  M  M  M  +  M  +  I  I  +  M  M                              |   4        |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                               1                                          |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                                        1                                 |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Zymbal's Gland                          | M  M  +  M  +  +  +  +  +  +  +  +  +  +  +                              |  12        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Renal Tubule, Dilatation, Diffuse    |    2                                                                     |      1  2.0|
      Renal Tubule, Dilatation, Focal      | 1           1              1  2  1     1                                 |      6  1.2|
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page   5                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 3| 7| 7|                             |            |
                                           | 8| 8| 8| 5| 8| 8| 5| 5| 8| 5| 5| 8| 6| 8| 8|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    VEHICLE                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1|                             |      L     |
    CONTROL                                | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 URINARY SYSTEM - cont                     |                                                                          |            |
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  M  +  +                              |  14        |
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   6                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 9| 7| 7| 7| 7| 7|                             |            |
                                           | 8| 5| 8| 5| 8| 8| 8| 8| 8| 8| 5| 8| 8| 8| 8|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    3125 PPM                               | 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|                             |      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |       1                             1  1                                 |      3  1.0|
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Blood Vessel                            | +                                                                        |   1        |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Subcapsular, Hyperplasia, Focal      | 1  1  1  1  1  1     1  1     1  1  1  1  1                              |     13  1.0|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  M  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  M  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  13        |
      Ectopic Thymus                       |          1                                                               |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page   7                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 9| 7| 7| 7| 7| 7|                             |            |
                                           | 8| 5| 8| 5| 8| 8| 8| 8| 8| 8| 5| 8| 8| 8| 8|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    3125 PPM                               | 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|                             |      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM - cont                     |                                                                          |            |
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hydrometra                           |                                           2                              |      1  2.0|
      Endometrium, Hyperplasia, Cystic     | 1  2  2  2  2  2  1  2  2     1  2  2  1  1                              |     14  1.6|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  A  +  +  +  M  +                              |  13        |
      Hyperplasia                          |    2     2                                                               |      2  2.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
      Hyperplasia                          |                                        2                                 |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | +  +  +  +  +  +  +  +  M  M  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Depletion Cellular, Diffuse          |                            4                                             |      1  4.0|
      Hematopoietic Cell Proliferation     |       2        1     1           2                                       |      4  1.5|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  M  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                             Page   8                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 9| 7| 7| 7| 7| 7|                             |            |
                                           | 8| 5| 8| 5| 8| 8| 8| 8| 8| 8| 5| 8| 8| 8| 8|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    3125 PPM                               | 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|                             |      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                      1                                                   |      1  1.0|
      Renal Tubule, Dilatation, Diffuse    |                   2                    2                                 |      2  2.0|
      Renal Tubule, Dilatation, Focal      | 1     1        1     1  1                                                |      5  1.0|
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   9                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 1| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7|                             |            |
                                           | 8| 5| 8| 8| 5| 5| 8| 8| 5| 5| 8| 8| 5| 8| 8|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    6250 PPM                               | 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4|                             |      L     |
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                   1     1           1  1                                 |      4  1.0|
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  I  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  I  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  I  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  I  +  +  +  +                              |  14        |
      Hyperplasia, Focal                   |                      1                                                   |      1  1.0|
      Subcapsular, Hyperplasia, Focal      | 1  1  1  1  1  1  1     1  1     1  1  1  1                              |     13  1.0|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  I  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  +  +  +  I  +  M  +  +  +  +                              |  13        |
      Pars Intermedia, Hypertrophy         |                                        1                                 |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | M  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
      Ectopic Thymus                       |                                           1                              |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  I  +  +  +  +                              |  14        |
      Atrophy                              |                            3                                             |      1  3.0|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  10                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 1| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7|                             |            |
                                           | 8| 5| 8| 8| 5| 5| 8| 8| 5| 5| 8| 8| 5| 8| 8|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    6250 PPM                               | 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4|                             |      L     |
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM - cont                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Endometrium, Hyperplasia, Cystic     | 2  1  2  2  1  2  3  1  2  2  1  1  1  2  2                              |     15  1.7|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | M  +  +  +  +  +  +  M  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  M  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | +  +  +  +  +  +  +  +  +  +  M  +  M  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hematopoietic Cell Proliferation     |                                        2                                 |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  I  +  +  +  +                              |  14        |
      Hyperplasia                          |                                     2                                    |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  M  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Skin                                    |                         +                                                |   1        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    |                            +                                             |   1        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  I  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  11                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 1| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7|                             |            |
                                           | 8| 5| 8| 8| 5| 5| 8| 8| 5| 5| 8| 8| 5| 8| 8|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    6250 PPM                               | 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4|                             |      L     |
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 RESPIRATORY SYSTEM - cont                 |                                                                          |            |
   Lung                                    | +  +  +  +  +  +  +  +  +  +  I  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Renal Tubule, Dilatation, Diffuse    |    1           2           1                                             |      3  1.3|
      Renal Tubule, Dilatation, Focal      |                                        1  1                              |      2  1.0|
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  12                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 8| 8| 5| 5| 8| 8| 5| 5| 5| 8| 5| 8| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    12500                                  | 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6|                             |      L     |
    PPM                                    | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                1  1  1        1  1  1                                    |      6  1.0|
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hyperplasia, Focal                   |    1                                                                     |      1  1.0|
      Subcapsular, Hyperplasia, Focal      | 1  1  1  1  1  1  1  1  1  1  1  1  1  1  1                              |     15  1.0|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  I                              |  14        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  I  +  +  +  +  +  +  +  +  +                              |  14        |
      Ectopic Thymus                       |    1  1                             1                                    |      3  1.0|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  13                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 8| 8| 5| 5| 8| 8| 5| 5| 5| 8| 5| 8| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    12500                                  | 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6|                             |      L     |
    PPM                                    | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM - cont                     |                                                                          |            |
      Endometrium, Hyperplasia, Cystic     | 2  2  2  2  2  2     2  2  2  2  1  2  1  2                              |     14  1.9|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hyperplasia                          |       2                                                                  |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  M  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | +  M  +  +  +  +  +  +  +  M  +  M  +  +  +                              |  12        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hematopoietic Cell Proliferation     |             3                 1        1                                 |      3  1.7|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hyperplasia, Atypical, Focal         |    2                                                                     |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Medulla, Cyst Epithelial Inclusion   |                            1                                             |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  14                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 8| 8| 5| 5| 8| 8| 5| 5| 5| 8| 5| 8| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    12500                                  | 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6|                             |      L     |
    PPM                                    | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |       1                                                                  |      1  1.0|
      Renal Tubule, Dilatation, Focal      | 1  1  1        1                    1                                    |      5  1.0|
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  15                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 8| 8| 5| 8| 8| 5| 8| 5| 8| 8| 8| 8| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    25000                                  | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|                             |      L     |
    PPM                                    | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  |                               +                                          |   1        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |    1                 1     1  2                                          |      4  1.3|
      Hepatocyte, Necrosis, Focal          |                   1  1                                                   |      2  1.0|
      Hepatocyte, Vacuolization            |                                                                          |            |
          Cytoplasmic, Focal               |                               1                                          |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hyperplasia, Focal                   | 1                                                                        |      1  1.0|
      Subcapsular, Hyperplasia, Focal      | 1  1  1  1  1  1  1  1  1  1  1  1  1  1  1                              |     15  1.0|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  I  +  +  +  M  +  +  M  +  I  +  +  +  +                              |  11        |
      Pars Intermedia, Hypertrophy         |             1                    1                                       |      2  1.0|
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  I  +  +  +  +  +  +  +  M  +  +  +  +  +                              |  13        |
      Ectopic Thymus                       |                                     1                                    |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  16                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 8| 8| 5| 8| 8| 5| 8| 5| 8| 8| 8| 8| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    25000                                  | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|                             |      L     |
    PPM                                    | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Endometrium, Hyperplasia, Cystic     | 2  2  2  2  2  2  2  2  2  2  2  1  2  2  2                              |     15  1.9|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lymph Node                              |                               +                                          |   1        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Congestion                           |                         1                                                |      1  1.0|
      Hyperplasia                          |                         1                                                |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | +  +  +  +  +  I  +  +  M  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hematopoietic Cell Proliferation     |             1                                                            |      1  1.0|
      Pigmentation                         |                                     1                                    |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  M  +  M  +  M  +  +                              |  12        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  17                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 8| 8| 5| 8| 8| 5| 8| 5| 8| 8| 8| 8| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    25000                                  | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|                             |      L     |
    PPM                                    | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                                  1                                       |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Renal Tubule, Dilatation, Focal      |          1        1           1     1                                    |      4  1.0|
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  18                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 8| 5| 8| 8| 8| 8| 5| 5| 5| 8| 8| 5| 8| 8|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    50000                                  | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9|                             |      L     |
    PPM                                    | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  M  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Gallbladder                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                         1  2  1  1                                       |      4  1.3|
      Necrosis, Focal                      |          1                                                               |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |    2  1     2     1  2  2     2  2                                       |      8  1.8|
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Tongue                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                             Page  19                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 8| 5| 8| 8| 8| 8| 5| 5| 5| 8| 8| 5| 8| 8|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    50000                                  | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9|                             |      L     |
    PPM                                    | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Blood Vessel                            | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Subcapsular, Hyperplasia, Focal      | 1  1  1  1  1  1  1  1  1  1  1  2  1  1  1                              |     15  1.1|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | +  +  +  +  +  +  M  +  M  +  +  +  M  M  +                              |  11        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  M  +  +  +  +  +  +  +  +  +                              |  14        |
      Pars Intermedia, Hypertrophy         |             2                          2                                 |      2  2.0|
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Ectopic Thymus                       |                   1              1                                       |      2  1.0|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Endometrium, Hyperplasia, Cystic     | 2  1  2  1  2  2  2  2  2  2  2  1  2  3  2                              |     15  1.9|
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  20                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 8| 5| 8| 8| 8| 8| 5| 5| 5| 8| 8| 5| 8| 8|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    50000                                  | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9|                             |      L     |
    PPM                                    | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM - cont                     |                                                                          |            |
   Vagina                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  M  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hematopoietic Cell Proliferation     |                                           2                              |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hyperplasia                          | 3                                                                        |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Femur, Fibrous Osteodystrophy, Focal |                   2                                                      |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Skeletal Muscle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  21                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 8| 5| 8| 8| 8| 8| 5| 5| 5| 8| 8| 5| 8| 8|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) FEMALE                  | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                             |      A     |
    50000                                  | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9|                             |      L     |
    PPM                                    | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Larynx                                  | I  +  M  M  +  +  I  I  I  +  I  I  I  +  I                              |   5        |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                      1                                                   |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                            1  1     1                                    |      3  1.0|
      Inflammation, Chronic Active         | 2                                                                        |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Zymbal's Gland                          | +  +  +  +  +  +  M  +  +  M  +  +  M  M  +                              |  11        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Glomerulus, Hyalinization            |                   1                                                      |      1  1.0|
      Renal Tubule, Dilatation, Focal      |    1  1     1                       1                                    |      4  1.0|
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  22                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 1| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 5| 4| 4| 4| 4| 4| 5| 4| 2| 4| 4| 4| 4| 4|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |      A     |
    VEHICLE                                | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|                             |      L     |
    CONTROL                                | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Gallbladder                             | I  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |          1                       1                                       |      2  1.0|
      Inflammation, Chronic                |    1                                                                     |      1  1.0|
      Inflammation, Chronic Active         |                            1  1           1                              |      3  1.0|
      Necrosis, Focal                      |    1                                2                                    |      2  1.5|
      Hepatocyte, Periportal,              |                                                                          |            |
          Vacuolization Cytoplasmic,       |                                                                          |            |
          Diffuse                          |                                  1     1                                 |      2  1.0|
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       | 2  1     1        1  1        1  2     1  1                              |      9  1.2|
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  23                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 1| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 5| 4| 4| 4| 4| 4| 5| 4| 2| 4| 4| 4| 4| 4|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |      A     |
    VEHICLE                                | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|                             |      L     |
    CONTROL                                | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Tongue                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Blood Vessel                            | +  +  +  +  +  +  +  +  +  +  +  +  +  M  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Atrophy                              | 2  1  2  2  2  1  1  1  2     2  1  1  1  1                              |     14  1.4|
      Subcapsular, Hyperplasia, Focal      |             1                                                            |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | +  M  +  M  M  M  M  +  M  M  +  +  M  +  M                              |   6        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  +  I  +  +  I  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  24                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 1| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 5| 4| 4| 4| 4| 4| 5| 4| 2| 4| 4| 4| 4| 4|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |      A     |
    VEHICLE                                | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|                             |      L     |
    CONTROL                                | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM - cont                     |                                                                          |            |
   Preputial Gland                         | M  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Prostate                                | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Rete Testes, Inflammation, Focal     |                   3                                                      |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
      Hyperplasia, Plasma Cell             |                                           1                              |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | +  +  M  +  +  +  +  +  +  A  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Atrophy                              |                                     2     1                              |      2  1.5|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M                              |            |
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  25                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 1| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 5| 4| 4| 4| 4| 4| 5| 4| 2| 4| 4| 4| 4| 4|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |      A     |
    VEHICLE                                | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|                             |      L     |
    CONTROL                                | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 MUSCULOSKELETAL SYSTEM - cont             |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Skeletal Muscle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Peripheral Nerve                        |                                     +                                    |   1        |
      Vacuolization Cytoplasmic, Diffuse   |                                     1                                    |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Spinal Cord                             |                                     +                                    |   1        |
      Vacuolization Cytoplasmic, Focal     |                                     2                                    |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Larynx                                  | M  +  M  M  M  M  M  M  M  M  +  M  M  +  +                              |   4        |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
      Inflammation, Acute                  |             1                                                            |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |    1     1                    1     1     1                              |      5  1.0|
                                           |__________________________________________________________________________|____________|
   Zymbal's Gland                          | +  +  +  +  +  +  +  +  +  +  M  +  M  M  +                              |  12        |
 _____________________________________________________________________________________________________________________|            |
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                             Page  26                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 1| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 5| 4| 4| 4| 4| 4| 5| 4| 2| 4| 4| 4| 4| 4|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |      A     |
    VEHICLE                                | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|                             |      L     |
    CONTROL                                | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
      Nephropathy                          |       1        1     1                 1                                 |      4  1.0|
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +                              |  14        |
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  27                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 4| 4| 5| 4| 5| 4| 4| 5| 5| 4| 5| 5| 4| 4|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |      A     |
    3125 PPM                               | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4|                             |      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |    1     1                                                               |      2  1.0|
      Necrosis, Focal                      |             1                                                            |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         |                                        +                                 |   1        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                                        2                                 |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Atrophy                              | 1  2  1  1  1  1  1  2  1  1  1  1  1  1                                 |     14  1.1|
      Hypertrophy, Focal                   |    1                                                                     |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  I  +  +  I  +  +  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  28                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 4| 4| 5| 4| 5| 4| 4| 5| 5| 4| 5| 5| 4| 4|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |      A     |
    3125 PPM                               | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4|                             |      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM - cont                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Epithelium, Degeneration, Focal      |                                           3                              |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Mineralization, Focal                |                                           1                              |      1  1.0|
      Germinal Epithelium, Degeneration,   |                                                                          |            |
           Diffuse                         |                                        2                                 |      1  2.0|
      Germinal Epithelium, Degeneration,   |                                                                          |            |
           Focal                           |                                           1                              |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hyperplasia                          |                2                    2  1                                 |      3  1.7|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  M  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | +  +  +  +  +  +  +  +  M  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M                              |            |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  29                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 4| 4| 5| 4| 5| 4| 4| 5| 5| 4| 5| 5| 4| 4|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |      A     |
    3125 PPM                               | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4|                             |      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 NERVOUS SYSTEM - cont                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Ventricle, Hydrocephalus             |                            3                                             |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |    1                                                                     |      1  1.0|
      Renal Tubule, Dilatation, Diffuse    |                         1        1                                       |      2  1.0|
      Renal Tubule, Dilatation, Focal      | 1                                   1                                    |      2  1.0|
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  30                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 1| 7| 7| 1| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 4| 5| 4| 4| 5| 4| 0| 5| 4| 4| 5| 4| 4|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |      A     |
    6250 PPM                               | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5|                             |      L     |
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                   1           1  1     1                                 |      4  1.0|
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         |                         +                                                |   1        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Atrophy                              | 1     2  1  1     2  2     1  1  1  2  1  1                              |     12  1.3|
      Hypertrophy, Focal                   | 1                 1  1                                                   |      3  1.0|
      Subcapsular, Hyperplasia, Focal      |    1  1                                                                  |      2  1.0|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | I  I  +  +  +  +  I  +  I  +  +  +  +  +  +                              |  11        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  31                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 1| 7| 7| 1| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 4| 5| 4| 4| 5| 4| 0| 5| 4| 4| 5| 4| 4|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |      A     |
    6250 PPM                               | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5|                             |      L     |
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM - cont                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | I  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | +  +  M  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M                              |            |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hydrocephalus                        | 2                                                                        |      1  2.0|
      Corpus Callosum, Medulla,            |                                                                          |            |
          Vacuolization Cytoplasmic, Focal |                1                                                         |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  32                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 1| 7| 7| 1| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 4| 5| 4| 4| 5| 4| 0| 5| 4| 4| 5| 4| 4|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |      A     |
    6250 PPM                               | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5|                             |      L     |
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 RESPIRATORY SYSTEM - cont                 |                                                                          |            |
      Inflammation, Chronic Active, Diffuse|                         3                                                |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     |             +                                                            |   1        |
      Developmental Malformation           |             3                                                            |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  33                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 4| 4| 4| 4| 4| 4| 5| 4| 4| 4| 5| 4| 5| 4|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |      A     |
    12500                                  | 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7|                             |      L     |
    PPM                                    | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       | 1                                                                        |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Atrophy                              |       1  1  1  1  1  1     2  2  1  1  1  1                              |     12  1.2|
      Hypertrophy, Focal                   |                            1     1                                       |      2  1.0|
      Subcapsular, Hyperplasia, Focal      | 1                                                                        |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | M  +  M  I  I  I  +  +  I  I  +  +  +  +  +                              |   8        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  I                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  34                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 4| 4| 4| 4| 4| 4| 5| 4| 4| 4| 5| 4| 5| 4|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |      A     |
    12500                                  | 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7|                             |      L     |
    PPM                                    | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM - cont                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Germinal Epithelium, Degeneration,   |                                                                          |            |
           Focal                           |                                     2                                    |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lymph Node                              |                                     +                                    |   1        |
      Iliac, Pigmentation, Focal           |                                     1                                    |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hyperplasia                          |                            2           2                                 |      2  2.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  I  +  +  +  M  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | +  +  M  +  +  +  M  +  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Thymus                                  | M  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M                              |            |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  35                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 4| 4| 4| 4| 4| 4| 5| 4| 4| 4| 5| 4| 5| 4|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |      A     |
    12500                                  | 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7|                             |      L     |
    PPM                                    | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 RESPIRATORY SYSTEM - cont                 |                                                                          |            |
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |          1                                                               |      1  1.0|
      Renal Tubule, Dilatation, Diffuse    |                   1                                                      |      1  1.0|
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  36                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 4| 4| 9| 4| 5| 4| 4| 4| 5| 4| 5| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |      A     |
    25000                                  | 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8|                             |      L     |
    PPM                                    | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +                              |  14        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |       1                             1                                    |      2  1.0|
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         |                               +                                          |   1        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                               1                                          |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Atrophy                              | 2  1  1  1        1     1     1  1     3  1                              |     10  1.3|
      Hypertrophy, Focal                   |                                        1                                 |      1  1.0|
      Subcapsular, Hyperplasia, Focal      |                            1        1                                    |      2  1.0|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  M  +  I  A  +  +  I  +  +  +  +  +  I  +                              |  10        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  37                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 4| 4| 9| 4| 5| 4| 4| 4| 5| 4| 5| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |      A     |
    25000                                  | 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8|                             |      L     |
    PPM                                    | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM - cont                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Prostate                                | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Testes                                  | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  M  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  M  +  +  +  +  +  +  +  +  +  +                              |  14        |
      Hyperplasia                          |                            2                                             |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | +  +  +  +  M  +  +  M  +  +  +  +  M  +  +                              |  12        |
      Hyperplasia                          |                               1                                          |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +                              |  14        |
      Hematopoietic Cell Proliferation     |                      2                                                   |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Atrophy, Diffuse                     |             4                                                            |      1  4.0|
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M                              |            |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  38                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 4| 4| 9| 4| 5| 4| 4| 4| 5| 4| 5| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |      A     |
    25000                                  | 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8|                             |      L     |
    PPM                                    | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +                              |  14        |
      Perivascular, Infiltration Cellular, |                                                                          |            |
           Lymphocyte                      |                               1     1                                    |      2  1.0|
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +                              |  14        |
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  39                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 5| 4| 5| 1| 4| 5| 4| 4| 4| 4| 4| 4| 4|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|                             |      A     |
    50000                                  | 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|                             |      L     |
    PPM                                    | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Gallbladder                             | +  +  M  +  +  +  +  +  +  +  M  +  M  +  +                              |  12        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       | 1     1           1           1        1                                 |      5  1.0|
      Inflammation, Chronic Active         |                                           1                              |      1  1.0|
      Necrosis, Focal                      |          1                                                               |      1  1.0|
      Hepatocyte, Periportal,              |                                                                          |            |
          Vacuolization Cytoplasmic,       |                                                                          |            |
          Diffuse                          |                               3  2  2     1                              |      4  2.0|
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       | 1     1  1  1     1  1  1  1        1  1                                 |     10  1.0|
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  40                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 5| 4| 5| 1| 4| 5| 4| 4| 4| 4| 4| 4| 4|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|                             |      A     |
    50000                                  | 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|                             |      L     |
    PPM                                    | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Tongue                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Tooth                                   |       +                                                                  |   1        |
      Peridontal Tissue, Cyst              |       X                                                                  |      1     |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Blood Vessel                            | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Atrophy                              | 1     1  1  2     1  1  2  1  1  2  1  2  1                              |     13  1.3|
      Hypertrophy, Focal                   | 1                       1           1                                    |      3  1.0|
      Subcapsular, Hyperplasia, Focal      |                   1                                                      |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | +  +  +  M  +  M  +  +  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  I  I  +  +  +  +  +  +  M  +  +                              |  12        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  41                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 5| 4| 5| 1| 4| 5| 4| 4| 4| 4| 4| 4| 4|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|                             |      A     |
    50000                                  | 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|                             |      L     |
    PPM                                    | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM - cont                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Preputial Gland                         | +  +  +  +  +  +  +  M  +  M  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hyperplasia                          |                      2                                                   |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hyperplasia, Plasma Cell             |                                     2  1                                 |      2  1.5|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | +  M  +  +  +  +  +  +  +  +  +  M  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hematopoietic Cell Proliferation     |    3           3                                                         |      2  3.0|
      Hyperplasia, Lymphoid                |                         1                                                |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  I  +  +  +  +  +  +  +  +  +                              |  14        |
      Atrophy, Focal                       |    1                                3                                    |      2  2.0|
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M                              |            |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  42                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 5| 4| 5| 1| 4| 5| 4| 4| 4| 4| 4| 4| 4|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|                             |      A     |
    50000                                  | 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|                             |      L     |
    PPM                                    | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 INTEGUMENTARY SYSTEM - cont               |                                                                          |            |
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Skeletal Muscle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Medulla, Vacuolization Cytoplasmic,  |                                                                          |            |
           Focal                           |                1                                                         |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Larynx                                  | M  +  M  M  M  M  M  I  M  M  I  M  +  M  M                              |   2        |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |       1                                                                  |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                                     1                                    |      1  1.0|
      Inflammation, Chronic Active         |                         1                                                |      1  1.0|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  43                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: 26-39 WEEKS                         TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:36:07    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 5| 4| 5| 1| 4| 5| 4| 4| 4| 4| 4| 4| 4|                             |            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      O     |
   P53+/-(C57BL/6) MALE                    | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|                             |      A     |
    50000                                  | 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|                             |      L     |
    PPM                                    | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 SPECIAL SENSES SYSTEM - cont              |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Zymbal's Gland                          | +  M  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Nephropathy                          |                               1  1        1                              |      3  1.0|
      Renal Tubule, Dilatation, Diffuse    |          1                                                               |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | +  +  M  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  44                                                               
                                                                                                                                   
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