TDMS Study 88004-06 Pathology Tables
NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC DIVINYLBENZENE Date: 08/14/03
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 13:01:09
FINAL #1 MICE
Facility: Battelle Northwest
Chemical CAS #: 1321-74-0
Lock Date: 06/17/02
Cage Range: All
Reasons For Removal: All
Removal Date Range: All
Treatment Groups: Include All
a Number of animals examined microscopically at site and number of animals with lesion
Page 1
NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC DIVINYLBENZENE Date: 08/14/03
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 13:01:09
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 10 PPM 30 PPM 100 PPM
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 50 50 50 50
Early Deaths
Moribund Sacrifice 11 12 8 7
Natural Death 6 3 3 1
Accidently Killed 1
Survivors
Terminal Sacrifice 33 35 38 42
Animals Examined Microscopically 50 50 50 50
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Esophagus (50) (50) (50) (50)
Infiltration Cellular, Mixed Cell 1 (2%)
Gallbladder (45) (41) (36) (41)
Hemorrhage, Chronic 1 (2%)
Inflammation, Acute 1 (3%)
Inflammation, Chronic 1 (2%)
Intestine Large, Rectum (46) (48) (50) (50)
Artery, Inflammation, Chronic Active 1 (2%)
Liver (49) (50) (50) (50)
Angiectasis 1 (2%)
Basophilic Focus 4 (8%) 2 (4%) 2 (4%)
Clear Cell Focus 4 (8%) 2 (4%) 3 (6%)
Eosinophilic Focus 12 (24%) 8 (16%) 3 (6%) 4 (8%)
Fatty Change 2 (4%) 1 (2%)
Hematopoietic Cell Proliferation 1 (2%) 1 (2%) 1 (2%)
Infarct 1 (2%)
Inflammation, Acute 1 (2%)
Inflammation, Chronic 1 (2%)
Inflammation, Granulomatous 1 (2%) 1 (2%)
Mixed Cell Focus 1 (2%)
Necrosis 1 (2%) 3 (6%) 1 (2%)
Tension Lipidosis 1 (2%) 5 (10%) 2 (4%) 4 (8%)
Vacuolization Cytoplasmic, Focal 1 (2%)
Centrilobular, Hypertrophy 1 (2%)
Mesentery (17) (16) (4) (5)
Artery, Inflammation, Chronic Active 1 (6%)
Fat, Congestion 1 (6%)
Fat, Hemorrhage 1 (25%)
Fat, Necrosis 17 (100%) 15 (94%) 3 (75%) 5 (100%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 2
NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC DIVINYLBENZENE Date: 08/14/03
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 13:01:09
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 10 PPM 30 PPM 100 PPM
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Pancreas (48) (50) (50) (50)
Atrophy 2 (4%) 1 (2%)
Basophilic Focus 1 (2%)
Duct, Cyst 2 (4%)
Stomach, Forestomach (50) (50) (50) (50)
Hyperplasia, Squamous 3 (6%) 5 (10%) 1 (2%)
Inflammation, Chronic Active 1 (2%) 2 (4%)
Ulcer 1 (2%) 1 (2%)
Stomach, Glandular (49) (50) (49) (49)
Hemorrhage 1 (2%)
Necrosis 1 (2%)
Tooth (9) (13) (8) (15)
Incisor, Dysplasia 9 (100%) 13 (100%) 8 (100%) 15 (100%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Blood Vessel (1)
Aorta, Mineralization 1 (100%)
Heart (50) (50) (50) (50)
Cardiomyopathy 3 (6%) 6 (12%) 1 (2%) 1 (2%)
Infiltration Cellular, Polymorphonuclear 1 (2%)
Inflammation, Suppurative 1 (2%)
Mineralization 1 (2%) 1 (2%) 1 (2%)
Necrosis 1 (2%)
Thrombosis 1 (2%)
Artery, Inflammation, Chronic Active 2 (4%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (50) (50) (50) (50)
Hematopoietic Cell Proliferation 1 (2%)
Hyperplasia 1 (2%) 2 (4%) 5 (10%) 4 (8%)
Hypertrophy 3 (6%) 2 (4%) 8 (16%)
Adrenal Medulla (49) (50) (49) (50)
Hyperplasia 1 (2%) 1 (2%) 2 (4%)
Necrosis 1 (2%)
Islets, Pancreatic (48) (49) (49) (50)
Hyperplasia 1 (2%)
Pituitary Gland (47) (50) (49) (45)
Cyst 1 (2%)
Pars Distalis, Angiectasis 3 (6%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 3
NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC DIVINYLBENZENE Date: 08/14/03
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 13:01:09
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 10 PPM 30 PPM 100 PPM
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM - CONT
Pars Distalis, Hyperplasia 8 (17%) 16 (32%) 6 (12%) 5 (11%)
Thyroid Gland (49) (49) (50) (48)
C-Cell, Hyperplasia 1 (2%)
Follicular Cell, Hyperplasia 1 (2%) 2 (4%) 1 (2%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Ovary (48) (50) (49) (49)
Angiectasis 2 (4%) 3 (6%)
Cyst 9 (19%) 14 (28%) 10 (20%) 9 (18%)
Inflammation, Acute 1 (2%)
Mineralization 1 (2%)
Necrosis 1 (2%)
Thrombosis 1 (2%)
Uterus (49) (50) (50) (49)
Angiectasis 1 (2%) 2 (4%) 1 (2%)
Endometrium, Hyperplasia, Cystic 2 (4%) 8 (16%) 5 (10%) 8 (16%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Lymph Node (9) (3) (5) (2)
Ectasia 1 (11%)
Deep Cervical, Hyperplasia, Lymphoid 1 (11%)
Deep Cervical, Infiltration Cellular, Plasma
Cell 1 (50%)
Iliac, Angiectasis 1 (50%)
Iliac, Ectasia 1 (11%)
Lumbar, Angiectasis 1 (20%)
Renal, Angiectasis 1 (20%)
Lymph Node, Mesenteric (49) (50) (49) (49)
Angiectasis 1 (2%)
Ectasia 1 (2%)
Inflammation, Granulomatous 1 (2%)
Spleen (49) (50) (49) (49)
Hematopoietic Cell Proliferation 3 (6%) 3 (6%) 3 (6%) 1 (2%)
Inflammation, Acute 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 4
NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC DIVINYLBENZENE Date: 08/14/03
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 13:01:09
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 10 PPM 30 PPM 100 PPM
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM - CONT
Necrosis 1 (2%)
Sinusoid, Dilatation 1 (2%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Skin (50) (50) (50) (50)
Inflammation, Chronic Active 3 (6%) 2 (4%) 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
None
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (50) (50) (50) (50)
Degeneration, Focal 1 (2%)
Hemorrhage 1 (2%)
Meninges, Infiltration Cellular, Mononuclear
Cell 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Larynx (48) (50) (50) (49)
Hyperplasia, Squamous 1 (2%)
Inflammation, Suppurative 1 (2%) 1 (2%)
Lung (50) (50) (50) (49)
Hemorrhage 1 (2%)
Infiltration Cellular, Polymorphonuclear 1 (2%)
Inflammation, Acute 1 (2%)
Thrombosis 1 (2%)
Alveolar Epithelium, Hyperplasia 4 (8%) 3 (6%) 4 (8%) 8 (16%)
Alveolus, Granuloma 1 (2%)
Alveolus, Infiltration Cellular, Histiocyte 3 (6%) 6 (12%) 9 (18%) 17 (35%)
Bronchiole, Hyperplasia 1 (2%)
Bronchiole, Hyperplasia, Atypical 39 (78%) 45 (90%) 48 (98%)
Nose (50) (50) (50) (49)
Inflammation, Suppurative 1 (2%) 50 (100%) 49 (98%) 49 (100%)
Glands, Respiratory Epithelium, Metaplasia 3 (6%) 50 (100%) 50 (100%) 49 (100%)
Olfactory Epithelium, Atrophy 8 (16%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 5
NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC DIVINYLBENZENE Date: 08/14/03
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 13:01:09
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 10 PPM 30 PPM 100 PPM
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM - CONT
Olfactory Epithelium, Degeneration, Hyaline 2 (4%) 50 (100%) 40 (80%) 8 (16%)
Olfactory Epithelium, Respiratory Epithelium,
Metaplasia 50 (100%) 50 (100%) 49 (100%)
Trachea (49) (50) (50) (50)
Inflammation, Suppurative 1 (2%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Eye (50) (50) (50) (49)
Cataract 1 (2%) 2 (4%) 1 (2%)
Inflammation 1 (2%)
Phthisis Bulbi 1 (2%) 1 (2%)
Cornea, Hyperplasia, Squamous 1 (2%)
Cornea, Inflammation, Chronic Active 1 (2%) 3 (6%) 3 (6%)
Cornea, Inflammation, Suppurative 1 (2%)
Cornea, Mineralization 6 (12%)
Harderian Gland (50) (50) (50) (50)
Hyperplasia 1 (2%) 4 (8%) 2 (4%) 3 (6%)
Inflammation, Chronic Active 1 (2%)
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (49) (50) (50) (50)
Amyloid Deposition 1 (2%)
Infarct 1 (2%)
Inflammation, Suppurative 2 (4%) 2 (4%)
Metaplasia, Osseous 2 (4%) 3 (6%) 3 (6%) 1 (2%)
Mineralization 1 (2%)
Nephropathy 25 (51%) 31 (62%) 22 (44%) 17 (34%)
Artery, Inflammation, Chronic Active 1 (2%)
Urinary Bladder (49) (50) (50) (49)
Inflammation, Suppurative 1 (2%)
Artery, Inflammation, Chronic Active 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 6
NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC DIVINYLBENZENE Date: 08/14/03
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 13:01:09
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE CONTROL 10 PPM 30 PPM 100 PPM
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 50 50 50 50
Early Deaths
Moribund Sacrifice 7 6 5 4
Natural Death 2 6 3 3
Survivors
Terminal Sacrifice 41 38 42 43
Animals Examined Microscopically 50 50 50 50
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Liver (50) (50) (50) (50)
Angiectasis 1 (2%)
Basophilic Focus 3 (6%) 6 (12%) 7 (14%) 4 (8%)
Clear Cell Focus 9 (18%) 11 (22%) 6 (12%) 5 (10%)
Eosinophilic Focus 8 (16%) 7 (14%) 2 (4%)
Fatty Change 2 (4%) 1 (2%)
Infarct 2 (4%) 1 (2%)
Inflammation, Granulomatous 1 (2%)
Mineralization 1 (2%)
Mixed Cell Focus 2 (4%) 1 (2%) 2 (4%) 1 (2%)
Necrosis 1 (2%) 2 (4%) 2 (4%) 2 (4%)
Tension Lipidosis 2 (4%) 1 (2%) 2 (4%) 1 (2%)
Vacuolization Cytoplasmic, Focal 2 (4%)
Mesentery (11) (4) (4) (5)
Fat, Necrosis 10 (91%) 4 (100%) 4 (100%) 5 (100%)
Pancreas (49) (48) (50) (50)
Duct, Cyst 2 (4%)
Stomach, Forestomach (49) (50) (49) (50)
Hyperplasia, Squamous 1 (2%) 4 (8%) 2 (4%) 4 (8%)
Inflammation 1 (2%)
Inflammation, Acute 2 (4%) 1 (2%)
Inflammation, Chronic Active 1 (2%) 1 (2%) 3 (6%)
Mineralization 1 (2%)
Ulcer 2 (4%) 1 (2%) 1 (2%)
Stomach, Glandular (48) (48) (47) (49)
Inflammation, Acute 1 (2%)
Mineralization 1 (2%)
Necrosis 1 (2%) 1 (2%)
Tooth (41) (43) (41) (35)
Incisor, Dysplasia 41 (100%) 43 (100%) 41 (100%) 35 (100%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 7
NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC DIVINYLBENZENE Date: 08/14/03
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 13:01:09
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE CONTROL 10 PPM 30 PPM 100 PPM
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (50) (49) (50) (50)
Cardiomyopathy 14 (28%) 5 (10%) 5 (10%) 5 (10%)
Mineralization 1 (2%) 1 (2%) 1 (2%)
Artery, Inflammation, Chronic Active 1 (2%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (49) (49) (50) (50)
Hyperplasia 9 (18%) 10 (20%) 6 (12%) 5 (10%)
Hypertrophy 24 (49%) 25 (51%) 26 (52%) 24 (48%)
Adrenal Medulla (49) (49) (50) (50)
Hyperplasia 1 (2%) 2 (4%) 2 (4%)
Parathyroid Gland (35) (39) (36) (35)
Hyperplasia 1 (3%)
Pituitary Gland (48) (47) (47) (46)
Cyst 1 (2%)
Pars Distalis, Hyperplasia 2 (4%) 1 (2%) 2 (4%) 1 (2%)
Thyroid Gland (49) (49) (50) (50)
Follicular Cell, Hyperplasia 2 (4%) 1 (2%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Epididymis (50) (50) (50) (50)
Granuloma Sperm 1 (2%) 2 (4%)
Penis (1)
Inflammation, Acute 1 (100%)
Preputial Gland (50) (50) (50) (50)
Ectasia 1 (2%) 3 (6%) 2 (4%) 1 (2%)
Inflammation, Chronic Active 2 (4%)
Seminal Vesicle (49) (48) (50) (49)
Inflammation, Chronic 1 (2%)
Testes (50) (50) (50) (50)
Atrophy 2 (4%)
Mineralization 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 8
NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC DIVINYLBENZENE Date: 08/14/03
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 13:01:09
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE CONTROL 10 PPM 30 PPM 100 PPM
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM - CONT
Interstitial Cell, Hyperplasia 1 (2%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (50) (48) (50) (50)
Necrosis 1 (2%)
Spleen (49) (48) (50) (50)
Hematopoietic Cell Proliferation 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Skin (50) (50) (50) (50)
Cyst Epithelial Inclusion 1 (2%)
Inflammation, Chronic Active 3 (6%) 1 (2%) 2 (4%)
Inflammation, Granulomatous 1 (2%)
Subcutaneous Tissue, Cyst Epithelial
Inclusion 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
None
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (50) (50) (50) (50)
Necrosis, Focal 1 (2%)
Artery, Inflammation, Chronic Active 1 (2%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Larynx (50) (50) (50) (50)
Inflammation, Suppurative 1 (2%)
Lung (49) (49) (49) (49)
Hemorrhage 1 (2%) 1 (2%)
Mineralization 1 (2%)
Alveolar Epithelium, Hyperplasia 1 (2%) 5 (10%) 5 (10%) 7 (14%)
Alveolus, Infiltration Cellular, Histiocyte 2 (4%) 4 (8%) 5 (10%) 1 (2%)
Bronchiole, Hyperplasia 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 9
NTP Experiment-Test: 88004-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC DIVINYLBENZENE Date: 08/14/03
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 13:01:09
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE CONTROL 10 PPM 30 PPM 100 PPM
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM - CONT
Bronchiole, Hyperplasia, Atypical 38 (78%) 46 (94%) 46 (94%)
Bronchiole, Inflammation, Chronic Active 1 (2%)
Nose (50) (50) (49) (50)
Inflammation, Suppurative 3 (6%) 47 (94%) 49 (100%) 49 (98%)
Glands, Necrosis 1 (2%)
Glands, Respiratory Epithelium, Metaplasia 12 (24%) 50 (100%) 49 (100%) 50 (100%)
Olfactory Epithelium, Atrophy 14 (28%)
Olfactory Epithelium, Degeneration, Hyaline 5 (10%) 50 (100%) 48 (98%) 11 (22%)
Olfactory Epithelium, Respiratory Epithelium,
Metaplasia 1 (2%) 50 (100%) 49 (100%) 50 (100%)
Respiratory Epithelium, Metaplasia, Squamous 1 (2%)
Sinus, Foreign Body 1 (2%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Eye (49) (47) (47) (50)
Phthisis Bulbi 1 (2%) 1 (2%)
Cornea, Inflammation, Chronic Active 2 (4%) 1 (2%) 1 (2%)
Cornea, Mineralization 2 (4%)
Harderian Gland (50) (49) (50) (50)
Hyperplasia 2 (4%) 2 (4%) 3 (6%) 2 (4%)
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (50) (50) (50) (50)
Cyst 1 (2%) 2 (4%)
Infarct 1 (2%) 2 (4%) 1 (2%)
Inflammation, Suppurative 1 (2%)
Metaplasia, Osseous 7 (14%) 3 (6%) 5 (10%) 1 (2%)
Nephropathy 45 (90%) 43 (86%) 40 (80%) 34 (68%)
Capsule, Fibrosis 1 (2%)
Papilla, Necrosis 1 (2%)
Pelvis, Dilatation 1 (2%)
Renal Tubule, Hyperplasia 2 (4%) 1 (2%)
Renal Tubule, Necrosis 1 (2%)
Urinary Bladder (48) (48) (49) (49)
Transitional Epithelium, Hyperplasia 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 10
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