National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/40429

Background Review Documents: Review of In Vitro Endocrine Disruptor Screening Assays


These background review documents (BRDs) contain data, a proposed list of substances, and minimum procedural standards that were reviewed by an independent Expert Panel in May 2002. Please refer to the final report, ICCVAM Evaluation of In Vitro Test Methods For Detecting Potential Endocrine Disruptors: Estrogen Receptor and Androgen Receptor Binding and Transcriptional Activation Assays (NIH Publication No. 03-4503), and its Addendum, for the final ICCVAM recommended substances and minimum procedural standards.

Estrogen Receptor Binding

Final ER Binding BRD - NIH Publication No. 03-4504 
Please note: This is a large document (5.7 Mb) and may take some time to open. The individual sections provided below may download faster and provide improved accessibility.

Main Body

Appendix A - Methods for In Vitro ER Binding Assays

Appendix B - In Vitro ER Binding Assay Protocols

Appendix C - Chemical and Product Class Information for the Substances Tested in the In Vitro ER Binding Assays 

Appendix D - Substances Tested in the In Vitro ER Binding Assays

Appendix E - Assay Distribution of Substances Tested in In Vitro ER Binding Assays 

Appendix F - Median/Single RBA Values for Substances Tested in Two or More In Vitro ER Binding Assays 

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Estrogen Receptor Transcriptional Activation

Final ER TA BRD - NIH Publication No. 03-4505 
Please note: This is a large document (4.8 Mb) and may take some time to open.The individual sections provided below may download faster and provide improved accessibility.

Main Body

Appendix A - Methods for In Vitro ER TA Assays

Appendix B - In Vitro ER TA Assay Protocols

Appendix C - Chemical and Product Class Information for Substances Tested in ER TA Assays 

Appendix D - Substances Tested in the In Vitro ER TA Assays

Appendix E - Responses of Substances Tested in at Least Two Different Types of In Vitro ER TA Assays 

Appendix F - Substances Tested in Different In Vitro ER TA Assays by the Same Investigator 

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Androgen Receptor Binding

Final AR Binding BRD - NIH Publication No. 03-4506
Please note: This is a large document (3.8 Mb) and may take some time to open.

Appendix A - Methods for In Vitro AR Binding Assays

Appendix B - In Vitro AR Binding Assay Protocols

Appendix C - Chemical and Product Class Information for the Substances Tested in the In Vitro AR Binding Assays 

Appendix D - Substances Tested in the In Vitro AR Binding Assays

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Androgen Receptor Transcriptional Activation

Final AR TA BRD - NIH Publication No. 03-4507
Please note: This is a large document (4.4 Mb) and may take some time to open. The individual sections provided below may download faster and provide improved accessibility.

Main Body

Appendix A - Methods for In Vitro AR TA Assays

Appendix B - In Vitro AR TA Assay Protocols

Appendix C - Chemical and Product Class Information for Substances Tested in AR TA Assays 

Appendix D - Data for Substances Tested in the In Vitro AR TA Assays

Appendix E - Substances Tested in Two or More Selected In Vitro AR TA Assays

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