Endocrine System

Pituitary Gland

    Narrative
    <strong>Figure 1.</strong> A semithin section (0.5 micrometer thick) of a toluidine blue O-stained area of the adenohypophysis (anterior lobe of the pituitary gland). There is a thin capsule of connective tissue (1, arrow) surrounding loose cords of endocrine secretory cells (4), capillaries (2), and intercordal spaces (3, short arrows). 25x.
    Figure 1. A semithin section (0.5 micrometer thick) of a toluidine blue O-stained area of the adenohypophysis (anterior lobe of the pituitary gland). There is a thin capsule of connective tissue (1, arrow) surrounding loose cords of endocrine secretory cells (4), capillaries (2), and intercordal spaces (3, short arrows). 25x.
    <strong>Figure 2.</strong> A low-power electron micrograph of a capillary lined with a thin endothelium (1), intercellular spaces (2), an intercordal space (3) with connective tissue elements, a somatotroph cell with a single nucleus (4), and numerous secretory granules of similar sizes. Two precursor cells (5) with scant cytoplasm and rare secretory granules are present. 1900x.
    Figure 2. A low-power electron micrograph of a capillary lined with a thin endothelium (1), intercellular spaces (2), an intercordal space (3) with connective tissue elements, a somatotroph cell with a single nucleus (4), and numerous secretory granules of similar sizes. Two precursor cells (5) with scant cytoplasm and rare secretory granules are present. 1900x.
    <strong>Figure 3.</strong> A higher magnification view of the adenohypophysis. A probable somatotroph cell (1) is present with numerous fairly uniform-sized secretory granules (3) and a well-developed field of stacked rough endoplasmic reticulum (2, short arrows). A thyrotroph cell with many large round mitochondria (4) and small secretory granules is present (5, long arrows). An intercellular space (6) is shown, as well as a capillary (7) lined with a thin endothelium. Finally, a cell with highly variable secretory granule sizes is consistent with a gonadotroph cell (8). 4800x.
    Figure 3. A higher magnification view of the adenohypophysis. A probable somatotroph cell (1) is present with numerous fairly uniform-sized secretory granules (3) and a well-developed field of stacked rough endoplasmic reticulum (2, short arrows). A thyrotroph cell with many large round mitochondria (4) and small secretory granules is present (5, long arrows). An intercellular space (6) is shown, as well as a capillary (7) lined with a thin endothelium. Finally, a cell with highly variable secretory granule sizes is consistent with a gonadotroph cell (8). 4800x.
    <strong>Figure 4.</strong> Another higher magnification image showing a corticotrophic cell (1) with a cluster of small mitochondria (2) and a thin band of small secretory granules (3) around the periphery of the cell. An intercellular space (4) with some membranous debris is shown. A somatotroph cell is present with a round nucleus (5), stacks of rough endoplasmic reticulum (6), numerous secretory granules (7), and sparse mitochondria (8). 4800x.
    Figure 4. Another higher magnification image showing a corticotrophic cell (1) with a cluster of small mitochondria (2) and a thin band of small secretory granules (3) around the periphery of the cell. An intercellular space (4) with some membranous debris is shown. A somatotroph cell is present with a round nucleus (5), stacks of rough endoplasmic reticulum (6), numerous secretory granules (7), and sparse mitochondria (8). 4800x.

    AUTHOR:

    Michael Dykstra, Ph.D.
    Electron Microscopy Consulting
    Beaufort, NC

    EDITOR:

    Kathleen A. Szabo, DVM, MS
    Senior Veterinary Pathologist
    Charles River Laboratories, Inc.
    Frederick, MD