Reproductive System, Male

Testis, Seminiferous Tubule - Retention, Spermatid

Narrative

Comment:

Spermiation (the release of fully mature spermatids into the tubular lumen) normally occurs during stage VIII of the spermatogenic cycle in rats and mice. Numerous toxicants have been shown to disrupt spermiation, resulting in the continued presence of the mature spermatids (step 19 spermatids in the rat, step 17 in the mouse) at the luminal surface of stage IX-XI tubules (Figure 1 and Figure 2). The retained spermatids are then pulled down into the basal Sertoli cell cytoplasm, where they are phagocytized. Spermatid retention has been described following exposure to a number of chemicals, including boric acid, 2,5-hexanedione, and dibromoacetic acid. Spermiation is testosterone dependant, so spermatid retention also occurs with decreased intratesticular levels of testosterone. Although subtle, this finding is important to recognize and record since it usually results in reduced epididymal sperm count, sperm abnormalities, and potential effects on fertility. A few retained spermatids can often be seen at the lumen of stage IX tubules or phagocytized at the base of stage XII tubules, but they are generally very few in number and restricted to one or two tubules.

Recommendations:

Spermatid retention should be diagnosed and graded and should be discussed in the pathology narrative if the incidence and/or severity appears to be related to chemical administration. Bilateral involvement should be diagnosed when present.

References:

Bryant BH, Yamasak I H, Sandrof MA, Boekelheide K. 2008. Spermatid head retention as a marker of 2,5-hexanedione-induced testicular toxicity in the rat. Toxicol Pathol 36:552-559.

Abstract: http://www.ncbi.nlm.nih.gov/pubmed/18467684

Creasy DM. 2001. Pathogenesis of male reproductive toxicity. Toxicol Pathol 29:64-76.

Full Text: http://tpx.sagepub.com/content/29/1/64.full.pdf

Creasy D, Bube A, de Rijk E, Kandori H, Kuwahara M, Masson R, Nolte T, Reams R, Regan K, Rehm S, Rogerson P, Whitney K. (2012). Proliferative and nonproliferative lesions of the rat and mouse male reproductive system. Toxicol Pathol 40:40S-121S.

Abstract: https://doi.org/10.1177/0192623312454337

Ku WW, Chapin RE, Wine RN, Gladen BC. 1993. Testicular toxicity of boric acid BA: Relationship of dose to lesion development and recovery in the F344 rat. Reprod Toxicol 7:305-319.

Abstract: http://www.ncbi.nlm.nih.gov/pubmed/8400621

Linder RE, Klinefelter GR, Strader LF, Suarez JD, Roberts NL, Dyer CJ. 1994. Spermatotoxicity of dibromoacetic acid in rats after 14 daily exposures. Reprod Toxicol 8:251-259.

Abstract: http://www.ncbi.nlm.nih.gov/pubmed/8075514

Linder RE, Klinefelter GR, Strader LF, Veermachaneni DNR, Roberts NL, Suarez JD. 1997. Histopathologic changes in testes of rats exposed to dibromoacetic acid. Reprod Toxicol 11:47-56.

Abstract: http://www.ncbi.nlm.nih.gov/pubmed/9138633

All Images

Testis, Seminiferous tubule - Retention, Spermatid in a male Sprague-Dawley rat. Retention of elongated spermatids is present in a stage XI seminiferous tubule. (Photograph courtesy of Dr. D. Creasy.)

Testis, Seminiferous tubule - Retention, Spermatid in a male Sprague-Dawley rat. Retention of elongated spermatids in the basal region of a stage XII seminiferous tubule. (Photograph courtesy of Dr. D. Creasy.)

Testis - Normal in a male Sprague-Dawley rat. Control stage XI tubule for comparison with Figure 1. (Photograph courtesy of Dr. D. Creasy.)

Testis - Normal in a male Sprague-Dawley rat. Control stage XII tubule for comparison with Figure 2. (Photograph courtesy of Dr. D. Creasy.)