Kidney, Renal Tubule - Accumulation, Hyaline Droplet





comment:
Hyaline droplets are eosinophilic homogeneous cytoplasmic droplets that are normally present in the P2 segment of proximal tubule epithelia of young, mature male rats. The droplets represent alpha 2u-globulin sequestered in secondary lysosomes ( Figure 1




recommendation:
Since the presence of hyaline droplets is a normal occurrence in younger male rats, the diagnosis of hyaline droplet accumulation in male rats in subchronic studies should be reserved for those cases in which there is an increase in the number of hyaline droplets relative to controls. In female rats, and male and female mice, hyaline droplet accumulation should be diagnosed whenever hyaline droplets are present. Hyaline droplet accumulation should be graded whenever it is diagnosed. Other changes seen concurrently and in association with hyaline droplet accumulation (e.g., granular cast formation, degeneration/necrosis of epithelial cells) should be diagnosed separately. The suspected pathogenesis (e.g., increased alpha 2U-globulin, association with neoplasia) should be discussed in the pathology narrative.related links:
Kidney, Renal Tubule - Degenerationreferences:
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De Rijk EPCT, Ravesloot WTM, Wijnands Y, van Esch E. 2003. A fast histochemical staining method to identify hyaline droplets in the rat kidney. Toxicol Pathol 31:462-464. Full Text: http://tpx.sagepub.com/content/31/4/462.full.pdf
Greaves P. 2012. Histopathology of Preclinical Toxicity Studies, 4th ed. Elsevier, Amsterdam, 560. Abstract: http://www.sciencedirect.com/science/book/9780444538567
Hard GC. 2008. Some aids to histological recognition of hyaline droplet nephropathy in ninety-day toxicity studies. Toxicol Pathol 36:1014-1017. Full Text: http://tpx.sagepub.com/content/36/7/1014.full
Hard GC, Snowden RT. 1991. Hyaline droplet accumulation in rodent kidney proximal tubules: An association with histiocytic sarcoma. Toxicol Pathol 19:88-97. Abstract: http://www.ncbi.nlm.nih.gov/pubmed/1722903
Lehman-McKeeman LD, Caudil D. 1992. Biochemical basis for mouse resistance to hyaline droplet nephropathy: Lack of relevance of the alpha 2u-globulin protein superfamily in this male rat-specific syndrome. Toxicol Appl Pharmacol 112:214-221. Abstract: http://www.ncbi.nlm.nih.gov/pubmed/1371614
Luz A, Murray AB. 1991. Hyaline droplet accumulation in kidney proximal tubules of mice with histiocytic sarcoma. Toxicol Pathol 19:670-671.
Short BG, Burnett VL, Cox MG, Bus JS, Swenberg JA. 1987. Site-specific renal cytotoxicity and cell proliferation in male rats exposed to petroleum hydrocarbons. Lab Invest 57:564-577. Abstract: http://www.ncbi.nlm.nih.gov/pubmed/3682766
Swenberg JM, Short B, Strasser J, Charbonneau M. 1989. The comparative pathobiology of α2u-globulin nephropathy. Toxicol Appl Pharmacol 97:35-46. Abstract: http://www.sciencedirect.com/science/article/pii/0041008X89900537
Uwagawa S, Saito K, Nakayama A, Umihira M, Okuno Y. 1990. Comparison of hyaline droplets in rats with chronic progressive nephropathy and chemical-induced α2u-globulin nephropathy. J Toxicol Pathol 5:195-203.
Yamate J, Iwaki M, Nakatsuji S, Kuwamura M, Kotani T, Sakuma S. 1998. Lysozyme-containing renal tubular hyaline droplets in F344 rats bearing a rat fibrosarcoma-derived transplantable tumor. Toxicol Pathol 26:699-703. Abstract: http://www.ncbi.nlm.nih.gov/pubmed/9789960
Web page last updated on: October 28, 2014